Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibacterial activity of seminalplasmin, a basic protein from bovine seminal plasma. | seminalplasmin, a 6,000 dalton antimicrobial protein present in bovine seminal plasma, is shown to inhibit growth and/or rna synthesis in several bacterial species. in only one strain out of twenty one belonging to fourteen species, did both rna synthesis and growth appear to be resistant to seminalplasmin. the antibacterial activity of seminalplasmin, in the case of e. coli, was also studied as a function of its concentration and of time; the minimal concentration of the protein required for 10 ... | 1986 | 2423852 |
| pseudoaneurysm of the left ventricle as a complication of septic embolism. | a case report of pseudoaneurysm (pa) of the left ventricle due to acute myocardial infarction (ami) caused by septic embolism from the infected aortic valve is presented. the patient was operated upon with resection of the left ventricular wall and insertion of a bioprosthesis in the aortic ostium. because of reoccurrence of pa 4 weeks postoperatively due to a pinhole defect in the left ventricular wall near the suture-line, reoperation was performed. the patient was discharged home and at follo ... | 1986 | 2429398 |
| [the capacity of anaerobically grown bacteria to exchange the 2h+ of the cell for the k+ of the medium and to maintain a high k+ distribution between cell and medium]. | the n,n'-dicyclohexylcarbodiimide sensitive exchange of 2h+ of a cell for k+ of medium stable to ph, k+ activity and temperature changes has been discovered in anaerobically grown gram-negative escherichia coli, salmonella typhimurium. s. enteritidis, proteus mirabilis, p. vulgaris, anaerobic gram-positive bacteria streptococcus faecalis, lactobacillus salivarius, l. lactis in the presence of exogenic energy source. this exchange in gram-negative bacteria is operating only at increase of medium ... | 1986 | 2434147 |
| effect of histological decalcifying agents on number and stainability of gram-positive bacteria. | in tests of the effects of restorative materials on dental pulp, it is important that one evaluate bacterial contamination, and this is usually done histologically. preceding the usual paraffin-embedding of hard-tissue specimens for microscopical investigations, decalcification is performed. to study the influence of decalcifying agents (nitric acid, formic acid, and ethylenediamine tetraacetic acid) on the number and gram-stainability of bacteria, we used a model system consisting of suspension ... | 1987 | 2440925 |
| sodium/proton antiporter in streptococcus faecalis. | streptococcus faecalis, like other bacteria, accumulates potassium ions and expels sodium ions. this paper is concerned with the pathway of sodium extrusion. earlier studies (d.l. heefner and f.m. harold, proc. natl. acad. sci. usa 79:2798-2802, 1982) showed that sodium extrusion is effected by a primary, atp-linked sodium pump. i report here that cells grown under conditions in which sodium atpase is not induced can still expel sodium ions. this finding suggested the existence of an alternate p ... | 1987 | 2442134 |
| studies on streptococcus faecalis enterotoxin. | streptococcus faecalis has been reported to cause food poisoning. six strains of s. faecalis were tested for sherman's criteria. these strains were non-hemolytic, dnase+ and ent+. the enterotoxin was purified on sephadex g-200 column and maximum activity was observed at 37 c and ph 7.0. enterotoxin treated with trypsin and papain elicited very poor response to fluid accumulation. the sensitivity of all the strains against different antibiotics was determined. strain 53 m was treated with acridin ... | 1987 | 2442976 |
| bacterial lysis of fibrin seal in vitro. | we performed an in vitro study to determine whether certain bacteria may lyse a commercially available fibrin seal (beriplast, behringwerke, marburg, frg). fibrinogen solution was mixed with actively growing bacterial cultures, and thrombin was added. during 20 days of incubation at 37 degrees c complete lysis was observed with a number of different bacteria, however, at different rates. complete lysis within 1-5 days was observed for the following species (number of strains in parentheses): str ... | 1988 | 2456934 |
| the influence of penicillin and quinaldofur on the growth of staphylococcus epidermidis and streptococcus faecalis in milk. | the influence of penicillin and quinaldofur on the growth of staphylococcus epidermidis and streptococcus faecalis in milk depends on aerobic conditions and the quality of milk. | 1987 | 2457295 |
| active potassium extrusion regulated by intracellular ph in streptococcus faecalis. | potassium extrusion in bacteria is thought to play a role in the regulation of the cytoplasmic ph; in several organisms, it has been ascribed to secondary antiport of k+ for protons. streptococcus faecalis exhibited a distinctive pattern: potassium extrusion occurred only when the cytoplasmic ph was alkaline and required the generation of atp. the key observation is that glycolyzing cells suspended in an alkaline medium extruded k+, even against a k+ concentration gradient, provided the medium c ... | 1988 | 2459118 |
| ion-conducting channels in a gram-positive bacterium. | the patch-clamp technique was used to obtain information on the existence and properties of ion channels in giant protoplasts obtained from the gram-positive bacterium streptococcus faecalis. the membrane proved to contain a pore with numerous conductance states, ranging from 10 ps to several nanosiemens. application of a slight pressure differential across the membrane resulted in the activation of the channel. the pressure sensitivity points to a relationship between this channel and one recen ... | 1988 | 2461319 |
| extrachromosomal control of antibiotic resistance, dnase activity and enterotoxin production in streptococcus faecalis. | 1988 | 2470242 | |
| effect of peptide as-48 on enterococcus faecalis subsp. liquefaciens s-47. | the enterococcal peptide as-48 exerts a concentration-dependent bactericidal effect on enterococcus faecalis subsp. liquefaciens s-47; cell rescue by cardiolipin and trypsin can be effected only in the first few minutes after antibiotic addition. gramicidin-exposed cells are protected from killing by as-48. long-term and pulse incorporation of radiolabeled substrates into trichloroacetic acid-precipitable material, o2 consumption, and the ability to maintain intracellular potassium levels are im ... | 1989 | 2473710 |
| proteinuria and haematuria as predictors of schistosomiasis in children. | examination of urine samples from 922 children from epe and surrounding communities in south-western nigeria indicated a 13% prevalence of schistosoma haematobium infection. children, 10-14 years of age, accounted for 65% of the disease prevalence. approximately 79% of the study population was negative for proteinuria, while 52.6% of children with 30 mg% proteinuria were positive for s. haematobium infection. however, 96% and 100% of all children who, respectively, had 30 mg% and 100 mg% protein ... | 1989 | 2475060 |
| the inner mitochondrial membrane contains ion-conducting channels similar to those found in bacteria. | patch-clamp experiments were performed on rat liver mitochondria inner membranes. application of voltage gradients of either polarity revealed the presence of several different conductances, ranging up to 1.3 ns in symmetrical 150 mm kcl. evidence is presented that at least those higher than 0.3 ns are substates of the highest conductance channel. increasing matrix-side-positive (unphysiological) transmembrane voltage gradients favored the switch of the 1.3 ns channel to operation in lower condu ... | 1989 | 2480918 |
| dna sequence analysis of bacterial toxic heavy metal resistances. | bacterial plasmids have genes that confer highly specific resistances to as, bi, cd, cu, cr, hg, pb, te, zn, and other toxic heavy metals. for each toxic cation or anion, generally a different resistance system exists, and these systems may be "linked" together on multiple resistance plasmids. for cd2+, aso2-, aso4(3)-, hg2+, and organomercurials, dna sequence analysis has supplemented direct physiological and biochemical experiments to produce sophisticated understanding. the cada atpase of s. ... | 1989 | 2484581 |
| [production of non-volatile amines by strains of enterococci]. | during the last few years there has been great interest concerning the problems related with the presence of pressor amines in different kinds of cheese, specially since the "cheese syndrome" was made evident. since the capacity of the bacterial groups involved in cheese manufacture to produce these amines has not yet been clarified, it was decided to study the production capacity of tyramine, tryptamine and histamine of enterococci strains probably used in the starter cultures. forty-one entero ... | 1989 | 2486213 |
| [activity of cefpirome against bacteria isolated from hospitalized patients]. | the activity of cefpirome (hr 810) was evaluated against 247 strains isolated from patients developing their infections while in a hospital in buenos aires. its activity against gram negative bacilli was compared with ceftriaxone, ceftazidime, cephalotin, piperacillin, amikacin, gentamicin and norfloxacin. in terms of mic50 and mic90 (mg/l) it was as follows: klebsiella pneumoniae: less than 0.125, less than 2.0; pseudomonas aeruginosa: less than 8, less than 16; escherichia coli: less than 0.01 ... | 1989 | 2487748 |
| effectiveness of single daily intraperitoneal administration of aztreonam and cefuroxime in the treatment of peritonitis in continuous ambulatory peritoneal dialysis (capd). | to discover if the management of peritonitis in continuous ambulatory peritoneal dialysis (capd) may be effectively simplified by single daily i.p. administration of aztreonam (a) and cefuroxime (c), 10 adult capd patients (pts) with peritonitis were trained to start the following treatment procedure: a) sterile collection of dialysate effluent for cultures; b) 4 rapid in-and-out exchanges with antibiotic free dialysate; c) addition of 2 g c and 2 g a to a 2-l exchange for 6-h dwell time (the sa ... | 1989 | 2488380 |
| [comparative study of the microbiological components in urinary infection (uti)]. | hundred-six-fifty-one samples of urine obtained from external (460 samples) and hospitalized (191 samples) patients with suspected urinary tract infections (uti) were analysed for bacterial flora isolation, identification of microorganisms and evaluation of their sensitivity to antimicrobial agents. 89 and 215 bacterial strains were identified in samples obtained respectively from external and hospitalized patients. e. coli was the dominant cause of community acquired uti (68.59%), while the cau ... | 1989 | 2488916 |
| [transfer of bacterial drug resistance factor in artificial dental plaque]. | transfer of drug resistance factor was examined using so-called artificial dental plaque. clinically isolated specimens of enterococcus faecalis showing various kind of drug resistance were used as the donors. streptococcus mutans bht strain was used as the recipient and the plaque former. s. mutans formed artificial dental plaque on the surface of glass sticks after 7 to 10 day cultivation in brain heart infusion medium containing 5% sucrose. each strain of e. faecalis was added to the plaque c ... | 1989 | 2489304 |
| [chemiluminescence response and phagocytic activity of murine polymorphonuclear leukocytes to various species of oral bacteria]. | polymorphonuclear leukocytes (pmn) are predominant cells in the gingival crevice and saliva, and may play an important role in oral bacteria. murine peritoneal pmn was used and stimulated with 9 genera, 17 species of oral bacteria, including cariogenic and periodontal pathogens. the pmn response to the bacteria was measured by the luminol mediated chemiluminescence (cl) response and phagocytic activities, and the activities of lysozyme in the reaction medium after the cl response were also measu ... | 1989 | 2489336 |
| hemagglutination activities of group b, c, d, and g streptococci: demonstration of novel sugar-specific cell-binding activities in streptococcus suis. | a total of 378 streptococcal isolates of lancefield groups b, c, d and g were tested for their ability to hemagglutinate untreated, sialidase-treated, and endo-beta-galactosidase-treated human erythrocytes. of the 43 strains showing positive hemagglutination, 9 were inhibitable with neutral monosaccharides. four strains were inhibited with galactose and n-acetylgalactosamine, whereas five were inhibited with galactose only. a third, sialic acid-specific adhesion activity was suggested for two ad ... | 1989 | 2492258 |
| effects of interleukin-1, lipopolysaccharide, and streptococci on procoagulant activity of cultured human cardiac valve endothelial and stromal cells. | fibrin is the primary constituent of the vegetation in infective endocarditis, and tissue factor expression is a major mechanism of coagulation activation on infected valves. to determine which cells may participate in coagulation activation in this setting, expression of procoagulant activity (pca; shown to be tissue factor) was studied in cultured endothelial and stromal cells derived from human cardiac valves. endothelial cells had negligible pca (99 +/- 50 mu/10(5) cells, mean +/- 1 standard ... | 1989 | 2492262 |
| ofloxacin susceptibility testing quality control parameters for microdilution and disk diffusion, and confirmation of disk diffusion interpretive criteria. | the susceptibilities of 221 clinical isolates to ofloxacin were tested simultaneously by broth microdilution and disk diffusion methods with commercially prepared 5-micrograms ofloxacin disks. the acceptability of the following previously proposed zone diameter breakpoints was confirmed: greater than or equal to 16 mm, susceptible; 13 to 15 mm, intermediate; less than or equal to 12 mm, resistant. on the basis of a multilaboratory collaborative study, the following are proposed as acceptable ofl ... | 1989 | 2492304 |
| synergism and mechanism of subinhibitory concentration of streptomycin on streptococcus faecalis. | the influence of various incubation conditions on synergism with cell wall-active agents combined with streptomycin was studied in a streptococcus faecalis strain. it could be shown that both a synergistic increased killing effect and a synergistic prolongation of post-antibiotic effect (pae) were not dependent on an active process, as they occurred both in an anaerobic atmosphere and when an electron transport inhibitor (sodium azide) was added to the media. moreover, it was demonstrated that i ... | 1989 | 2492428 |
| further studies on post-antibiotic effect and synergism in two strains of streptococcus faecalis. | the differences in the synergistic potential for either killing effect or increase in duration of post-antibiotic effect (pae) seen with various beta-lactam-aminoglycoside combinations in two strains of streptococcus faecalis were further studied to examine this relationship in more detail. it was seen that a strain which produced a synergistic increase in recovery period (pae greater than or equal to 0.5 h), comparing the beta-lactam antibiotics alone and when combined with streptomycin, showed ... | 1989 | 2492429 |
| intra-abdominal sepsis after hepatic resection. | one hundred and thirty hepatic resections performed over an 8-year period were reviewed for evidence of postoperative intra-abdominal sepsis. of 126 patients who survived for more than 24 hours after operation, 36 developed culture positive intra-abdominal collections (28.6%). significant independent variables associated with the development of intra-abdominal sepsis were diagnoses of trauma or cholangiocarcinoma, and the need for reoperation to control hemorrhage during the postoperative period ... | 1989 | 2493775 |
| endocarditis due to streptococcus faecalis with high-level resistance to gentamicin. | recently, reports of streptococcus faecalis with resistance to high levels of gentamicin have appeared in the literature, although there have been no previous reports of endocarditis due to such organisms. in this article, two cases of endocarditis--both due to streptococcus faecalis with high-level gentamicin resistance and both presenting in an atypical manner--are described. the data suggest that a high level of suspicion regarding a diagnosis of endocarditis must be maintained in such cases ... | 1989 | 2495566 |
| characterization of the human neutrophil response to sex pheromones from streptococcus faecalis. | synthetic analogs of sex pheromones from streptococcus faecalis and related pheromone inhibitors have been assayed for their possible effects on human neutrophil leukocyte activation. these sex pheromones are hydrophobic peptides that have regulatory roles in bacterial mating behavior leading to intercellular plasmid transfer. five of the seven peptides tested were chemotactic for neutrophils in the 10(-5) to 10(-6) m concentration range. exposure of neutrophils to these same peptides induced po ... | 1989 | 2495725 |
| stimulating effect of streptococcus faecalis on phagocytosis in gnotobiotic piglets. | the concentration of active phagocytes in peripheral blood remained in germfree pigs up to the age of one year approximately at the same level as found at the age of 7 d and did not exceed 0.3 x 10(9)/l of blood, whereas a steady increase was established in conventional pigs. monoassociation of gnotobiotic piglets with streptococcus faecalis increased during 24 h the concentration of circulating granulocytes and the concentration of active phagocytes. an even more pronounced effect was obtained ... | 1989 | 2496013 |
| in vitro activities of quinolones against enterococci resistant to penicillin-aminoglycoside synergy. | the mics and mbcs of ci-934, ciprofloxacin, difloxacin (a-56619), a-56620, norfloxacin, enoxacin, amifloxacin, and coumermycin were determined for 43 clinical isolates of enterococcus faecalis known to be resistant to penicillin-aminoglycoside synergy. results were compared with those obtained for 37 synergy-susceptible e. faecalis and 22 enterococcus faecium strains. although no substantial differences in quinolone activities were observed between synergy-resistant and -susceptible e. faecalis ... | 1989 | 2496659 |
| in vitro activity of ampicillin/sulbactam against enterococci determined by the time-kill method. | time-kill studies demonstrated that at clinically achievable serum concentrations, ampicillin/sulbactam was equivalent in activity to ampicillin alone against non-beta-lactamase producing isolates of enterococcus faecalis and enterococcus faecium. sulbactam possessed no antibacterial activity against these organisms. it is not yet known if the activity of ampicillin will be increased with the addition of sulbactam when tested against beta-lactamase-producing enterococci. | 1989 | 2496949 |
| polymicrobial septicemia with clostridium difficile in acute diverticulitis. | a case is reported of a patient without previous gastrointestinal symptoms, who presented with polymicrobial septicemia caused by escherichia coli, enterococcus faecalis, clostridium difficile and bacteroides vulgatus. septicemia occurred during acute diverticulitis. a strain of clostridium difficile, which was of the same serogroup c as the blood culture isolate and also produced toxin, was recovered from the stools, but the pathogenic role of this organism in the gastrointestinal symptomatolog ... | 1989 | 2497002 |
| streptococcus faecalis arthritis. | septic arthritis due to streptococcus faecalis is rare. we describe a case in which synovial biopsy was required for diagnosis and a new longterm antibiotic combination using ciprofloxacin and amoxicillin was successful in treating the infection. comparison is made with previous reported cases and the usual poor outcome in this condition. | 1989 | 2497255 |
| phosphoenolpyruvate:sugar phosphotransferase system of bacillus subtilis: nucleotide sequence of ptsx, ptsh and the 5'-end of ptsi and evidence for a ptshi operon. | the nucleotide sequence of a 1689bp fragment of the bacillus subtilis locus containing ptsx (a crr-like gene), ptsh (coding for hpr), and the 5'-end of ptsi (coding for enzyme i) was determined. the deduced amino acid sequences of ptsh and the n-terminal part of ptsi were compared to those of streptococcus faecalis and escherichia coli. transcription fusion demonstrated that ptshi constitutes an operon. an open reading frame overlapping the main part of ptsh in the opposite sense was shown to be ... | 1989 | 2497294 |
| partial nucleotide sequence of the pts operon in salmonella typhimurium: comparative analyses in five bacterial genera. | the nucleotide sequence of a salmonella typhimurium dna segment of 549 base pairs which encompasses the operator-promoter of the pts operon, the entirety of the ptsh gene, encoding hpr of the phosphotransferase system (pts), the first 29 nucleotides of the ptsi gene, encoding enzyme i of the pts, and the intercistronic region between the ptsh and ptsi genes was determined and compared with the corresponding sequence from escherichia coli (de reuse et al., 1985). the two sequences showed 91% over ... | 1989 | 2497295 |
| [streptococcus bovis septicemia and colonic cancer]. | the authors report the case of a patient who developed a streptococcus bovis septicemia in the post-operative course of a surgical cure of an incarcerated umbilical hernia. there were no other complaints. a barium enema showed a malignant tumor of the descending colon. there were hepatic metastases. no complementary treatment was undertaken. the patient died shortly after. in the literature colonic proliferative lesions in association with this germ is reported in a total of 36% of proliferative ... | 1989 | 2497613 |
| inducible, transferable resistance to vancomycin in enterococcus faecalis a256. | a strain of enterococcus faecalis (a256) was isolated from the urine of a patient with urinary sepsis and was found to exhibit susceptibilities (micrograms per milliliter) to various glycopeptides as follows: vancomycin, 256; teicoplanin, 16; 62208, 512; 62211, 4; and 62476, 16. as judged by growth rates before and after exposure to sub-mics of glycopeptides, vancomycin and 62476 induced self-resistance, 62208 and 62211 induced slight self-resistance, and teicoplanin did not induce self-resistan ... | 1989 | 2497704 |
| susceptibility and bactericidal activity studies of four beta-lactamase-producing enterococci. | beta-lactamase-producing (bla+) enterococci are rare but have been reported from several areas. we report another bla+ enterococcus with high-level gentamicin resistance. susceptibility and bactericidal activity studies of four bla+ enterococci against potential alternative antibiotics, including ampicillin-sulbactam, daptomycin, teicoplanin, and vancomycin, are presented. | 1989 | 2497706 |
| role of adherence in pathogenesis of enterococcus faecalis urinary tract infection and endocarditis. | enterococcus faecalis strains isolated from urinary tract infections (utis) and endocarditis were analyzed for their ability to adhere to urinary tract epithelial cells (ecs) and girardi heart (gh) and human embryonic kidney (hek) cell cultures. uti isolates adhered to urinary tract ecs more efficiently than to the cultured cells, at the same time showing the least affinity for gh cells. in contrast, endocarditis isolates adhered to gh cell cultures more readily than to urinary tract ecs. moreov ... | 1989 | 2498209 |
| synthesis of novel 5-fluoro analogues of norfloxacin and ciprofloxacin. | a series of polyfluoro-3-quinolonecarboxylic acids have been synthesized and their in vitro antibacterial activity evaluated. the desired 7-(substituted amino) derivatives were prepared from the 5,6,7,8-tetrafluoroquinolone acids. conversely, amine displacement occurred primarily at the 5-position when the ester was used. structure-activity studies indicated that the antibacterial activity was greatest when the n-1 substituent was cyclopropyl and the 7-substituent was 4-methyl-1-piperazinyl. all ... | 1989 | 2498519 |
| [factors of protracted and recurrent course of chronic erosions of the stomach]. | a study was made of the disease pathogenesis in 58 patients with recurrent chronic erosions of the gastric mucosa. it has been established that an important role in the relapses of the pathological process is played by pathological microflora, disturbances in humoral immunity and local microcirculation, and long existence of the zone of fibrinoid necrosis. | 1989 | 2499074 |
| purification and amino acid composition of peptide antibiotic as-48 produced by streptococcus (enterococcus) faecalis subsp. liquefaciens s-48. | peptide antibiotic as-48 was purified to homogeneity by ion-exchange chromatography, gel filtration chromatography, and reversed-phase liquid chromatography. the purified fraction was active against gram-positive and gram-negative bacteria. as-48 is a basic protein with an isoelectric point of ca. 10.5 and a molecular mass of 7.4 kilodaltons. its inhibitory activity was markedly affected by sodium dodecyl sulfate and cardiolipin but not by neuraminidase, pectinase, beta-glucosidase, or beta-gluc ... | 1989 | 2499249 |
| modification of homogeneous resistance in a methicillin-resistant strain of staphylococcus aureus by acquisition of a beta-lactamase encoding plasmid. | nearly all clinical isolates of methicillin-resistant staphylococcus aureus (mrsa) are heterogeneously resistant and produce beta-lactamases which generally are plasmid mediated. in order to study the role of beta-lactamase plasmids in the expression of methicillin resistance, a beta-lactamase plasmid from enterococcus faecalis hh22 (pbem10) was transferred into a homogeneously resistant, beta-lactamase-negative strain of mrsa (musc284). by single disc diffusion testing at 42 degrees c, beta-lac ... | 1989 | 2499563 |
| in-vitro and in-vivo activity of cefpirome (hr 810) against methicillin-susceptible and -resistant staphylococcus aureus and streptococcus faecalis. | with cefpirome (hr 810) the mics for both methicillin-resistant staphylococcus aureus (mrsa) and for streptococcus faecalis were 8.0 mg/l. at 4 x mic, cefpirome killed mrsa as rapidly as did vancomycin while for enterococci, cefpirome, vancomycin or ampicillin alone were not bactericidal. however, all agents were bactericidal when combined with 2 mg/l of gentamicin, although gentamicin combined with cefpirome showed a smaller decrease in cfu/ml than the combination with ampicillin or vancomycin. ... | 1989 | 2499565 |
| binding of fibronectin and albumin to enterococcus (streptococcus) faecalis. | binding of human fibronectin (fn) and albumin to enterococcus (streptococcus) faecalis was investigated. scatchard analysis showed that the cells could bind a maximum of 1300 molecules of fn with an association constant of 4.8 x 10(6) m-1. binding did not appear to involve lipoteichoic acid. heating the cells or pretreatment with protease or periodate reduced the binding, suggesting the involvement of protein and/or carbohydrate-containing components as the surface receptor. the cells could bind ... | 1989 | 2499743 |
| study on pathogenesis of enterococcus faecalis in urinary tract. | the rate of isolation of enterococcus faecalis as the causative bacterium of complicated urinary tract infections has been increasing. however, the pathogenicity of this bacterium in the urinary tract has not been clarified. thus, the authors investigated how e. faecalis is pathogenic to the urinary tract, using mice with experimental urinary tract infection. the investigation revealed that this bacterium when sufficiently numerous can be directly pathogenic. the bacterium can be pathogenic indi ... | 1989 | 2499973 |
| [reactivity of human lymphocytes to yeast-like and mold fungi]. | the capacity of the allergens of mold fungi (rhizopus nigricans, mucor pusillus, alternaria tenuis, cladosporum herbarum, fusarium oxysporum) and yeast-like fungi (candida albicans) to induce the production of lymphokins by human lymphocytes was studied. all these preparations were active in reactions with lymphocytes obtained from adult donors, but did not activate lymphocytes of newborns (obtained from umbilical blood). in equal doses (10 micrograms/ml) c. albicans allergen was more active tha ... | 1989 | 2500001 |
| 19-deformyl-4'-deoxydesmycosin (tmc-016): synthesis and biological properties of a unique 16-membered macrolide antibiotic. | 19-deformyl-4'-deoxydesmycosin was synthesized by the following synthetic route: 19-deformylation of desmycosin, 3,2',4''-tri-o-trimethylsilylation, 4'-o-sulfonylation, 4'-iodination, reductive deiodination and 3,2',4''-tri-o-detrimethylsilylation. deformylation of the aldehyde group at the c-19 position was achieved by two different methods: a) a simple one-step deformylation using wilkinson's catalyst ((ph3p)3rhcl). b) reductive decarboxylation of the 19-carboxyl derivative following naclo2 ox ... | 1989 | 2500413 |
| purification and characterization of human neutrophil peptide 4, a novel member of the defensin family. | primary (azurophil) granules of neutrophils contain proteins which play a major role in the killing and digestion of bacteria in the phagolysosome. we have isolated and characterized a novel antimicrobial peptide from the azurophil granule fraction of discontinuous percoll gradients. we have named this peptide human neutrophil peptide 4 (hnp-4) based on its structural similarity to a group of antimicrobial polypeptides known as defensins (hnp 1-3). using size exclusion and reverse-phase high per ... | 1989 | 2500436 |
| oral hygiene and the prevalence of oral group d streptococci in medically-physically compromised and periodontal disease patients. | the relationship between oral hygiene and oral group d streptococcal carrier status was examined in two groups of dental clinic patients. eighteen medically-physically compromised and 20 periodontitis patients were selected for study. oral hygiene status was assessed and the prevalence of oral group d streptococci was determined by sampling the oral cavity with a vigorous 15 second rinse with 5 ml peptone-saline solution. three ml of each sample was cultured at 40 degrees c in 30 ml of sf broth ... | 1989 | 2500512 |
| monoadduct forming photochemical reagents for labeling nucleic acids for hybridization. | we describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. these compounds were used to label nucleic acids in the presence of lysed cell constituents. the resulting labelled nucleic acids show hybridization to a genus specific probe for e. coli. the relative comparison of sensitivity indicates that a polyamine linker is better than a polyethylene oxide linker between the biotin and angelicin moieties. | 1989 | 2500642 |
| physiological studies on the growth and utilization of sugars by listeria species. | experiments, relevant to growth in milk, were done to delineate the aerobic and anaerobic growth of listeria species on selected sugars in several media. all species grew on glucose aerobically, forming lactic acid and (or) acetic acid. anaerobically, only lactic acid was formed; cell yields were 80% of those obtained aerobically. when incubated aerobically, small amounts (1.5 microns/ml) of isovaleric acid, 2-hydroxyisovaleric acid, and trace amounts of isobutyric acid were formed. these produc ... | 1989 | 2501014 |
| false resistance of streptococcus faecalis to enoxacin by disc diffusion susceptibility tests. | 1989 | 2501266 | |
| inducible, transferable resistance to vancomycin in enterococcus faecium, d399. | enterococcus faecium d399 was isolated from the blood and peritoneal abscess of a patient with intraabdominal sepsis. the patient had not been treated with vancomycin, but the strain was found to be resistant with a mic of 1000 mg/l. resistance was inducible and transferable (probably by conjugation) to jh2-2, and correlated with induction of synthesis of a 39 kda protein. this mechanism appears to be identical to that previously described for e. faecalis a256, suggesting that dissemination of t ... | 1989 | 2501270 |
| prevalence and susceptibility of highly gentamicin resistant enterococcus faecalis in a south london teaching hospital. | combinations of aminoglycosides plus ampicillin usually are necessary for the satisfactory management of serious enterococcus faecalis sepsis. a study in a south london hospital demonstrated that 7% of all e. faecalis, and 30% of those from blood cultures, were highly gentamicin resistant. addition of gentamicin confers no benefit to the treatment of these organisms with ampicillin. we looked at the susceptibility pattern of these organisms in vitro and concluded that ampicillin plus ciprofloxac ... | 1989 | 2501271 |
| the non-flavin redox center of the streptococcal nadh peroxidase. i. thiol reactivity and redox behavior in the presence of urea. | unlike the 2-electron-reduced (eh2) forms of the flavoprotein disulfide reductases and mercuric reductase, the native eh2 form of the streptococcal nadh peroxidase is quite refractile toward chemical modification with thiol-specific reagents. in the presence of 1.3 m urea, however, the single thiol of the reduced enzyme reacts with phenylmercuric acetate with a t1/2 of 3 min. this modification abolishes the charge-transfer absorbance band at 540 nm and inactivates the enzyme; the latter effect i ... | 1989 | 2501302 |
| the non-flavin redox center of the streptococcal nadh peroxidase. ii. evidence for a stabilized cysteine-sulfenic acid. | incubation of the streptococcal nadh peroxidase with 5-thio-2-nitrobenzoate under anaerobic denaturing conditions leads to the rapid incorporation of 1 eq/fad of the aromatic thiol. addition of dithiothreitol to the resulting conjugate, following ultrafiltration, demonstrates that a mixed disulfide has been formed. analysis of the denatured nadh peroxidase by iso-electric focusing reveals the presence of two predominant species differing in isoelectric point by approximately 0.1 units. preincuba ... | 1989 | 2501303 |
| sensibility assay for topical agents. a new method. | we present a new laboratory method to test the sensibility of clinically isolated strains to topical agents. it is a method of dilution of the whole cream in a solid medium. the cream is weighted, suspended in sterile water, and maintained at 45 degrees c. then a volume of that suspension is added to the agar, maintained at 45 degrees c also. the mixture is agitated and plated. afterwards 25 strains are inoculated by a multinoculator (cetin, bs. as., argentina). after incubation (18 hours at 37 ... | 1989 | 2501310 |
| microbiological quality and public health significance of ice-cream. | fifty samples of ice-cream were subjected to microbiological examination. of these 72% had total viable count over 10(6)/g while 66% had coliform count between 10(2)-10(3)/g. the micro-organisms isolated were escherichia coli (46%), enterobacter aerogenes (34%), staphylococcus aureus (26%), proteus species (16%), streptococcus faecalis (12%), citrobacter species (10%) and bacillus cereus (4%). the results showed that commercially prepared ice-cream sold in the market was not satisfactory for hum ... | 1989 | 2501524 |
| antibiotic proteins of human polymorphonuclear leukocytes. | nine polypeptide peaks with antibiotic activity were resolved from human polymorphonuclear leukocyte azurophil granule membranes. all but 1 of the 12 constituent polypeptides were identified by n-terminal sequence analysis. near quantitative recovery of protein and activity permitted an assessment of the contribution of each species to the overall respiratory-burst-independent antimicrobial capacity of the cell. three uncharacterized polypeptides were discovered, including two broad-spectrum ant ... | 1989 | 2501794 |
| bactericidal and bacteriolytic action of peptide antibiotic as-48 against gram-positive and gram-negative bacteria and other organisms. | a purified peptide antibiotic as-48 from streptococcus faecalis spp liquiefaciens s-48 exerted a bactericidal mode of action against most gram-positive and many gram-negative bacteria tested. in many gram-positive bacteria and the two myxococcus species assayed, a bacteriolytic effect, as a consequence of primary lesions, was also observed. in general, the gram-negative bacteria were more resistant to as-48. escherichia coli protoplasts showed increased sensitivity and those of a resistant yeast ... | 1989 | 2501837 |
| postantibiotic effect of penicillin plus gentamicin versus enterococcus faecalis in vitro and in vivo. | a persistent suppression of bacterial growth following a brief exposure to an antibiotic (postantibiotic effect [( pae]) has been described for a variety of antibiotics and microorganisms. if a pae is present in vivo, antibiotic levels in tissue at the site of infection may decrease below the mic without bacterial regrowth in the latter portion of the dosing interval. in the present studies, a pae was sought in vitro and in vivo for penicillin g plus gentamicin versus enterococcus faecalis. the ... | 1989 | 2502064 |
| effect of blood product medium supplements on the activity of cefotaxime and other cephalosporins against enterococcus faecalis. | the activities of cefotaxime and other aminothiazoyl oxime cephalosporins against enterococcus faecalis were enhanced by addition of 5% sheep blood to mueller-hinton agar. this effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and it was specific to the syn-configuration of the oxime moiety. enhancement of cefotaxime activity was demonstrable against approximately 50% of 86 clinical isolates and could only be shown a ... | 1989 | 2502357 |
| bacteriological study of choledochal bile in patients with common bile duct stones, with or without acute suppurative cholangitis. | acute suppurative cholangitis is a serious complication in extrahepatic biliary tract obstruction. bacteriological studies have shown that in these patients the bile cultures usually present several kinds of bacteria, especially gram-negative aerobic bacteria such as e. coli and klebsiella sp. the mortality rate in our patients with common bile duct stones and acute suppurative cholangitis is enhanced 5- to 10-fold. the aim of the present study was to analyse the bacterial properties of choledoc ... | 1989 | 2502489 |
| antibacterials. synthesis and structure-activity studies of 3-aryl-2-oxooxazolidines. 1. the "b" group. | the synthesis and structure/activity studies of the effect of varying the "b" group in a series of oxazolidinone antibacterials (i) are described. two synthetic routes were used: (1) alkylation of aniline with glycidol followed by dialkyl carbonate heterocyclization to afford i (a = h, b = oh), whose arene ring was further elaborated by using electrophilic aromatic substitution methodology; (2) cycloaddition of substituted aryl isocyanates with epoxides to give a and b with a variety of values. ... | 1989 | 2502627 |
| (3-amino-2-oxoalkyl)phosphonic acids and their analogues as novel inhibitors of d-alanine:d-alanine ligase. | the dipeptide d-alanyl-d-alanine is an essential precursor of bacterial peptidoglycan; thus, blocking its formation is a possible target for the design of novel antibacterial agents. the synthesis of this dipeptide by bacterial d-alanine:d-alanine ligase requires atp. in analogy with glutamine synthetase, we hypothesized a mechanism for this enzyme involving the intermediacy of d-alanyl phosphate. several (3-amino-2-oxoalkyl)phosphonic acids and their analogues have been synthesized as possible ... | 1989 | 2502630 |
| enhancement of survival from murine polymicrobial peritonitis with increased abdominal abscess formation. | muramyl dipeptide (mdp), a purified synthetic immune adjuvant, has been shown to increase murine intraabdominal abscess formation in a monomicrobial model using bacteroides fragilis. this effect required live bacteria and was abolished by appropriate antibiotics. a polymicrobial model of peritonitis and abdominal abscess formation using streptococcus fecalis, escherichia coli, and b. fragilis was initially used to determine mortality rates at various concentrations and obtain an appropriate ld50 ... | 1989 | 2502684 |
| bacteremia caused by a vancomycin-resistant enterococcus. | 1989 | 2502769 | |
| species-specific interpretive breakpoints for ciprofloxacin disk diffusion susceptibility testing. | when ciprofloxacin was introduced on the swedish market the recommended interpretive standards for disk diffusion susceptibility testing using a 10 microgram disk were: sensitive greater than or equal to 24 mm (mic less than or equal to 1 mg/l) and resistant less than 18 mm (mic greater than 4 mg/l), except for enterococci where s greater than or equal to 18 mm and r less than 14 mm were recommended. a quality control study revealed that the accuracy of the routine susceptibility testing of cipr ... | 1989 | 2502836 |
| [the sequence of specific immune reactions at the biliary level after oral vaccination]. | the appearance and the progression of the specific immunoreactions at biliary levels (agglutinant titre, immunoenzimatic titration of igagm, iga, igg) in rabbits treated per os from 1 to 10 times, daily or bi-weekly, with 20 and 40 milliard of inactivated bacterial antigens (e. coli, proteus vulgaris, staph. aureus, str. faecalis) have been studied. the appearance of the specific biliary antibodies has been very precocious (only one day after the first administration) and the values increased pr ... | 1989 | 2503788 |
| endocarditis due to streptomycin-susceptible enterococcus faecalis with high-level gentamicin resistance. | a case of community-acquired endocarditis caused by enterococcus (streptococcus) faecalis with high-level resistance to gentamicin sulfate but not to streptomycin sulfate is described. killing curves performed using achievable serum levels showed synergistic killing when streptomycin but not gentamicin, tobramycin, or amikacin was combined with penicillin g sodium or vancomycin hydrochloride. combination therapy with vancomycin and streptomycin resulted in cure. serum bactericidal levels indicat ... | 1989 | 2504123 |
| infective endocarditis in crohn's disease. | infective endocarditis developed in a patient with crohn's disease. this case highlights a hazard of immunosuppressive treatment in inflammatory bowel disease. | 1989 | 2504264 |
| intraperitoneal penetration of cefpirome. | twenty-six patients undergoing elective gastrointestinal surgery received a 1 g intravenous dose of cefpirome before operation. serum and peritoneal fluid samples, obtained 0.5-7.6 h following administration, were assayed for cefpirome by a microbiological assay. the serum half-life of cefpirome was 2.1 h. the mean concentration of cefpirome in peritoneal fluid 0-2 h after administration was 44.4 micrograms/ml. the half-life of cefpirome in peritoneal fluid was 2 h, with mean concentrations of l ... | 1989 | 2504599 |
| growth of gram-positive and gram-negative bacteria in platelet concentrates. | in 1986 the allowable platelet storage time was reduced from 7 to 5 days because of a recent increase in septic deaths associated with platelet transfusion. in this study, the growth curves of two gram-positive and two gram-negative organisms in platelets stored for 7 days in clx and pl-732 bags were evaluated. platelets in clx bags were inoculated with 10(1), 10(2), and 10(3) organisms and 10(2) organisms were introduced into pl-732 bags. test organisms were inoculated into trypticase soy broth ... | 1989 | 2505412 |
| effects of treatment of lambs with a probiotic, containing lactic acid bacteria. | 1989 | 2505427 | |
| identification of aggregation substances of enterococcus faecalis cells after induction by sex pheromones. an immunological and ultrastructural investigation. | the sex pheromone system of enterococcus faecalis is responsible for the clumping response of a plasmid carrying donor strain with a corresponding plasmid free recipient strain due to the production of sex pheromones by the recipient strain. the clumping response is mediated by a surface material (called aggregation substance) which is synthesized upon addition of sex pheromones to the cultures. here we show that after induction a dense layer of "hairlike" structures is formed on the cell wall o ... | 1989 | 2505719 |
| localization of aggregation substances of enterococcus faecalis after induction by sex pheromones. an ultrastructural comparison using immuno labelling, transmission and high resolution scanning electron microscopic techniques. | the distribution of sex pheromone induced aggregation substance was studied on the cell surface of various enterococcus faecalis strains. in the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. using direct and indirect immunogold technique, transmission electron microscopy and high resolution scanning electron microscopy we investigated the appearance and distribution of the aggregation substance. the "hairs" increase in number with incr ... | 1989 | 2505720 |
| high-level vancomycin-resistant enterococci causing hospital infections. | nosocomial infection or colonization due to enterococci with high-level resistance to vancomycin (minimal inhibitory concentrations [mics] between 64 and greater than 2000 mg/l) has occurred in 41 patients with renal disease. these vancomycin-resistant enterococci were cultured from many sources including blood. all but one strain contained one or more plasmids ranging in molecular weight from 1.0 to 40 megadaltons (mda). vancomycin resistance was transferable by conjugation to a susceptible rec ... | 1989 | 2506070 |
| the prevalence of high-level aminoglycoside resistance among enterococci isolated from blood cultures during 1980-1988. | two hundred and eighty-one strains of enterococci isolated from blood cultures of 281 consecutive patients during 1980-1988 and 108 strains of enterococci isolated from miscellaneous clinical materials during 1988 were studied for high-level resistance (mic greater than 2000 mg/l) to amikacin, kanamycin, streptomycin, gentamicin, tobramycin and netilmicin. before 1985 there was no enterococcal strain with high-level resistance to amikacin, gentamicin, tobramycin or netilmicin, but 14% were resis ... | 1989 | 2506165 |
| [the effect of roxithromycin on the aerobic fecal flora in children]. | to evaluate the effect of roxithromycin on aerobic flora in 14 children treated orally (5 mg/kg/day during 5 to 7 days) quantitative stool cultures were performed using selective media. enterobacteriaceae (eb), streptococci d (str d), staphylococcus aureus, candida were counted at days 0, 2 and 5 days, and 2 days after treatment. the mic of each strain was determined for roxithromycin, erythromycin and spiramycin. ten infants in a nursery had already received antibiotics. a moderate albeit signi ... | 1989 | 2506510 |
| [antibacterial activity of pefloxacin in the urine during the 7 days following a single 800 mg oral dose]. | antibacterial activity of pefloxacin was studied in the urine after a single 800 mg oral dose in ten healthy female volunteers. urine was collected in 9 periods: 0-6 h, 6-12 h, 12-24 h, 24-28 h, 48-72 h, 72-96 h, 96-120 h, 120-144 h, 144-168 h. pefloxacin concentrations were assayed in all samples by a microbiological method and by hplc. urine antibacterial activity was determined towards five bacterial strains isolated in urine: 2 e. coli strains, one sensitive and the other resistant to nalidi ... | 1989 | 2506514 |
| [antibacterial activity of lomefloxacin in the urine during the 4 days following a single 400 mg oral dose]. | antibacterial activity of lomefloxacin was studied in the urine after single dose of 400 mg in ten healthy female volunteers. urine was collected in 7 periods: 0-3 h, 3-6 h, 6-12 h, 12-24 h, 24-48 h, 48-72 h, 72-96 h. lomefloxacin concentration were assayed in all samples by microbiological method. urine antibacterial activity was determined towards five strains isolated in urine: 2 e. coli strains one sensitive and the other resistant to nalidixic acid (nal-a), 1 klebsiella pneumoniae resistant ... | 1989 | 2506515 |
| infected charcot spine following spinal cord injury. | 1989 | 2506651 | |
| treatment of experimental endocarditis caused by a beta-lactamase-producing strain of enterococcus faecalis with high-level resistance to gentamicin. | several antimicrobial regimens were evaluated in the treatment of experimental enterococcal endocarditis due to a beta-lactamase-producing, highly gentamicin-resistant strain of enterococcus faecalis. ampicillin alone cleared bacteremia in the majority of rats and reduced titers of bacteria within vegetations (6.84 versus 8.80 log10 cfu/g in controls) but did not sterilize valves. ampicillin-sulbactam combinations, vancomycin, daptomycin, and imipenem each reduced residual bacterial titers withi ... | 1989 | 2506803 |
| comparative in vitro activity of sm7338, a new carbapenem antimicrobial agent. | the comparative in vitro activity of sm7338 was tested against 670 routine clinical isolates and 130 cefoperazone-resistant isolates of bacteria by agar dilution methods. sm7338 was at least as active as imipenem against gram-negative organisms but was slightly less active against gram-positive organisms. sm7338 was particularly active against members of the family enterobacteriaceae, with mics for 90% of strains of less than or equal to 0.125 micrograms/ml for all species tested. differences in ... | 1989 | 2508543 |
| purification and characterisation of tyrosine decarboxylase and aromatic-l-amino-acid decarboxylase. | microbial tyrosine decarboxylase (ec 4.1.1.25) and mammalian aromatic-l-amino-acid decarboxylase (ec 4.1.1.28) catalyse the formation of tyramine from l-tyrosine. these enzymes were characterised after isolation to purity by methods including fast polymer liquid chromatography (fplc). tyrosine decarboxylase was isolated from streptococcus faecalis by fplc anion exchange chromatography (11-times purification; 72% recovery; 23.2 u/mg protein). fplc on phenyl-superose resulted in purification to 11 ... | 1989 | 2508758 |
| [in-vitro activity of enoxacin: a multicenter study]. | the in vitro activity of enoxacin was tested in 14 german microbiological centers shortly after the introduction of the drug in germany. 2748 unselected clinical isolates including 15 bacterial species were analysed using microtiter plates. the mic90-values were as follows: staphylococcus aureus 4 mg/l, enterococcus faecalis 16 mg/l, enterobacteriaceae 0.5 mg/l, pseudomonas aeruginosa 8 mg/l. there is good correlation between these results and those of former investigations. it is known that qui ... | 1989 | 2509373 |
| diagnosing endocarditis with the cloned 112 kda antigen of enterococcus faecalis. | a new indirect elisa is presented for the diagnosis of enterococcal endocarditis. it is based on the cloning of enterococcus faecalis dna into lambda gt11. the library was screened by antisera from three cases of e. faecalis endocarditis. three positive clones were found, all of which cross-reacted with the 112 kda antigen of e. faecalis. one of these was taken and lysogenised into e. coli y1089 to produce a fusion protein of 125 kda. this iptg dependent protein was purified by affinity chromato ... | 1989 | 2509565 |
| enterococcus faecalis resistance to drugs: recent trends and development of high-level resistance to aminoglycosides. | 1989 | 2509642 | |
| in vitro and in vivo antibacterial activities of bo-1341, a new antipseudomonal cephalosporin. | bo-1341, a new antipseudomonal semisynthetic cephalosporin, was evaluated for in vitro and in vivo antibacterial activities in comparison with ceftazidime, cefotaxime, and cefoperazone. the in vitro activity of bo-1341 was generally superior or comparable to the activities of the reference antibiotics against clinical isolates of the family enterobacteriaceae. bo-1341 was highly active against pseudomonas aeruginosa (mic for 90% of the strains tested, 1.56 micrograms/ml), pseudomonas maltophilia ... | 1989 | 2510590 |
| the streptococcal flavoprotein nadh oxidase. i. evidence linking nadh oxidase and nadh peroxidase cysteinyl redox centers. | the fad-containing nadh oxidase from streptococcus faecalis 10c1, which catalyzes the four-electron reduction of o2----2h2o, has been purified by an improved procedure for analyses of its structural and redox properties. the enzyme is apparently a dimer of two identical subunits, each containing 1 mol of fad. dithionite reduction of the enzyme proceeds in two distinct phases corresponding to approximately 0.5 and 1.1 eq/fad, respectively. thiol assays of the nadh oxidase, reduced anaerobically w ... | 1989 | 2511195 |
| the streptococcal flavoprotein nadh oxidase. ii. interactions of pyridine nucleotides with reduced and oxidized enzyme forms. | anaerobic addition of 0.5 eq of nadh/fad to the streptococcal nadh oxidase produces a redox form spectrally similar to that obtained with 0.5 eq of dithionite/fad. the second phase of the titration, however, in addition to reducing the flavin with 1 eq of nadh/fad, leads to the appearance of a long-wavelength absorbance band centered at 725 nm. reductive titrations of the enzyme with 3-acetylpyridine-adenine dinucleotide, which has a redox potential 72 mv more positive than that of nadh, yield a ... | 1989 | 2511196 |
| bacterial meningitis in parturients after epidural anesthesia. | 1989 | 2511785 | |
| postantibiotic effects of imipenem, norfloxacin, and amikacin in vitro and in vivo. | the postantibiotic effects (paes) of imipenem and norfloxacin were tested against staphylococcus aureus, streptococcus (enterococcus) faecalis, escherichia coli, and pseudomonas aeruginosa. amikacin was tested against the same bacteria except streptococcus faecalis. for in vitro tests, a viable count-washing method was used, and for in vivo tests, the thread technique in normal mice was used. all three drugs produced paes of 1.1 to 3.8 h in vitro and 1.4 to 4.3 h in vivo against the pathogens te ... | 1989 | 2511798 |
| pyogenic thyroiditis due to branchial pouch sinus. | suppurative thyroiditis due to branchial pouch sinus is a rare disease, affecting children and young adults. it presents as an abscess in the lower lateral neck, usually on the left side. suppurative thyroiditis can be suggested by ultrasound, radionuclide scintigraphy or ct scan, but the diagnosis can only be established by barium swallow showing the sinus from the apex of the pyriform sinus to the perithyroid region. complete surgical excision of the sinus is essential. two patients are presen ... | 1989 | 2512269 |
| crystallization and preliminary x-ray diffraction study of the flavoprotein nadh peroxidase from streptococcus faecalis 10c1. | nadh peroxidase from streptococcus faecalis 10c1 has been crystallized from ammonium sulfate solutions using the hanging drop vapor diffusion method. depending on ph, the crystals grew in the orthorhombic space group i222 or one of its subgroups p222 or p2(1)2(1)2 (or one of its two permutations). in both cases the unit cell axes are a = 76.6 a, b = 132.9 a, and c = 145.7 a. there are two monomers/asymmetric unit in the body-centered crystal form and four in the primitive one. the enzyme is cata ... | 1989 | 2512289 |
| serological identification of streptococcus salivarius subsp. thermophilus. | streptococcus salivarius subsp. thermophilus (str. thermophilus) has no group-specific antigen. hcl extracts of 551 strains of streptococci isolated from cheese or yogurt (123 str. thermophilus and 428 enterococcus faecalis - ent. faecium spp.) were examined with three type-specific antisera prepared against representatives of str. thermophilus and also with lancefield group d antisera. all typical (80) or atypical (43) strains of str. thermophilus reacted with at least one type-specific antiser ... | 1989 | 2512335 |
| susceptibility of enterococci and epidemiology of enterococcal infection in the 1980s. | 1989 | 2514108 | |
| gentamicin-resistant enterococci and endocarditis. | we report a case of endocarditis associated with a highly gentamicin-resistant enterococcus that failed to respond to conventional antibiotic combination therapy. clinical resolution was achieved following cardiac surgery and antimicrobial treatment dictated by in vitro testing of the patient's organism. laboratories should test blood culture isolates of enterococci for high level gentamicin resistance. management of such cases requires individual assessment and extensive laboratory investigatio ... | 1989 | 2514420 |