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evidence for the covalent linkage of carbohydrate polymers to a glycoprotein from streptococcus sanguis.the platelet aggregation-associated protein (paap) from streptococcus sanguis contains 39% carbohydrate in rhamnose-rich polysaccharides. synthesized by cultured protoplasts, these polysaccharides were shown to be covalently linked to this cell wall protein using specific inhibitors of glycosylation, glycosidase treatment, amino acid and carbohydrate analyses of prepared minimal glycopeptides and isolated protein, and nmr spectroscopy. to our knowledge, this is the first direct proof of an n-asp ...19938226911
migration of a streptococcus sanguis strain through the root dentinal tubules.the persistent presence of bacteria in the root canal system often leads to the failure of treatment. the aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. bovine dentin was inoculated with a strain of streptococcus sanguis, nctc 7863; samples were taken at 10, 20, and 28 days, and bacterial penetration of the tubules was demonstrated using two techniques, scanning electron microscopy and light microscopy after brown and brenn staining. the results sho ...19938228750
serum immunoglobulin g antibodies to porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum and streptococcus sanguis during experimental gingivitis in young adults.twenty-eight young, healthy adults completed an experimental gingivitis study in which blood and clinical recordings were obtained at baseline; after a 4-week period of thorough oral hygiene; after a subsequent 3-week period of plaque accumulation; and after another 2 weeks of thorough oral hygiene. serum immunoglobulin g antibodies against whole cells of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum and streptococcus sanguis were determined using enzyme-linked immunos ...19938233568
simulated human serum profiles of one daily dose of ceftriaxone plus netilmicin in treatment of experimental streptococcal endocarditis.we performed experiments in rats aimed at determining whether a combination of ceftriaxone (cro) and netilmicin (net), by using once-daily administration in rats, which simulated profiles of drug in human serum, was more effective than either agent alone in the treatment of endocarditis caused by viridans group streptococci. a programmable infusion pump system enabled the production of profiles of cro in serum that simulate those found in humans after the intravenous administration of 2 g. the s ...19938239615
the effect of glass-ionomer cement on carious dentine: an in vivo study.the effect of a glass-ionomer cement sealant on carious dentine was investigated clinically and bacteriologically. the study group consisted of 24 molar teeth, with small clinical visible occlusal dentine lesions, in 13 children (aged 7-18 years). twenty of these molars were filled and sealed with a glass-ionomer cement (fuji ionomer type iii) and 4 with a resin sealant (delton). from each molar, two dentine samples were collected aseptically with a time interval of 7 months. the first sample wa ...19938242680
evaluation of the capacity of oral streptococci to produce hydrogen peroxide.the capacity of 11 strains of oral streptococcal species (streptococcus sanguis, s. oralis, s. mitis and s. sobrinus) to produce hydrogen peroxide (h2o2) was studied in vitro. detection of this property in solid media, particularly with trypticase soy agar-benzidine-peroxidase, was more sensitive than in liquid media. the addition of carbohydrates (arabinose, xylose, mannose, sorbose and lactose), sorbitol and saccharine to buffered trypticase soy broth increased h2o2 production in s. oralis nct ...19938246261
alpha-hemolytic streptococcal bacteremia: a review of 203 episodes during 1980-1991.this is a review of 203 episodes of alpha-hemolytic streptococcal bacteremia during a 12-year period (1980-91) in a large community teaching hospital in the usa. there were 87 male and 116 female patients, with ages ranging from newborn infants to a 96-year-old; 118 patients (58.1%) were 60 years or older. 31 patients had endocarditis, 93 had bacteremia, and 79 had polymicrobial bacteremia. streptococcus sanguis, s. mitis, s. salivarius and s. intermedius accounted for 80% of the strains. some s ...19938248738
production and characterization of monoclonal antibodies against bacterial lectin of eikenella corrodens.a lectin-like substance (ecls) was purified from the eikenella corrodens 1073 cell and monoclonal antibodies were produced against it to confirm the role of ecls in adhesive properties of e. corrodens such as hemagglutination and coaggregation with oral bacteria. four hybridoma clones were selected. two of the antibodies were of the igg1 isotype and the others were of the igg2b isotype. these monoclonal antibodies inhibited both the hemagglutination of e. corrodens and the coaggregation with act ...19938254457
phenotypic characterization of oral streptococci by classical methods.the phenotypic characteristics of 215 strains of oral streptococci were compared on the basis of the results of sorbitol, raffinose and trehalose fermentation, ammonium from arginine production, aesculin hydrolysis, h2o2 production, susceptibility to 2 u bacitracin, and growth in 4% nacl solution. eleven different species were identified. among the most noteworthy findings were the low number of h2o2-producing strains of streptococcus sobrinus, the effective discrimination between streptococcus ...19938264436
coaggregation of oral lactobacilli with streptococci from the oral cavity.the ability of oral lactobacilli to coaggregate with streptococci and actinomycetes was investigated. of the 7 species of lactobacilli studied, only two were capable of coaggregation and the coaggregation was restricted to streptococci. lactobacillus salivarius strains (2/4) coaggregated with streptococcus salivarius, streptococcus gordonii, streptococcus crista and tufted streptococcus sanguis ii strains. lactobacillus fermentum (2/3) coaggregated with s. gordonii and s. sanguis. the coaggregat ...19938265207
antimicrobial activity of cytotoxic drugs may influence isolation of bacteria and fungi from blood cultures.the potential antimicrobial activity of cyclophosphamide, vincristine, and adriamycin against gram positive and negative bacteria and candida albicans was examined. the time taken for different microbial inocula to turn a simulated blood culture positive in the presence of different concentrations of these drugs was measured. doxorubicin retarded the growth of staphylococcus aureus, staphylococcus epidermidis, and streptococcus sanguis in a concentration dependent manner. cyclophosphamide and vi ...19938282838
role of serum antibodies to streptococci in patients with iga nephropathy.serum antibodies to whole cells of streptococci and serum immune complexes were investigated in patients with iga nephropathy accompanied by tonsillitis and in healthy adult controls by enzyme-linked immunosorbent assay (elisa). serum iga levels to streptococcus salivarius, s. sanguis i and s. pneumoniae were higher in patients with iga nephropathy than in healthy controls. the levels of igg to s. pyogenes t12, s. gordonii and s. sanguis ii in patients with iga nephropathy were also higher than ...19938285043
chelate root filling cements: biological properties.the purpose of this study was to test in vivo and in vitro the toxicity and the antibacterial activity of an experimental chelate cement (hn cement) using zinc oxide-eugenol cement as a reference. after subcutaneous injection of the spatulated hn cement paste in rats, it induced markedly less tissue injuries than did the zinc oxide-eugenol cement. in toxicity tests using cultures of human fibroblasts, the hn cement was found to be less toxic than the reference cement. bacteriologically, blood ag ...19938289021
grouping of oral streptococcal species using fourier-transform infrared spectroscopy in comparison with classical microbiological identification.the grouping and identification made by fourier-transform infrared spectroscopy (ft-ir) of 40 oral streptococcal strains was compared with their known taxonomic positions. grouping was obtained by cluster analysis on the spectral distances between the first derivative spectra of the strains. spectra were divided into five spectral 'windows' and cluster analysis was either on either a combination of all five windows or on a selected window. the oral streptococci were divided into two main cluster ...19938297255
isomalto-oligosaccharide-containing lipoteichoic acid of streptococcus sanguis. basic structure.the lipoteichoic acid of streptococcus sanguis dsm 20567 and of dsm 20068 was isolated by phenol/water extraction and hydrophobic-interaction chromatography. the preparations from both strains have an identical structure: a 1,3-linked poly(glycerophosphate) chain phosphodiester-linked to glc-(alpha 1-2)glc(alpha 1-3)acyl2gro as the lipid anchor. the chain is substituted with d-alanine ester and glycosyl residues which comprise mono-, di-, tri- and tetra-alpha-d-glucopyranosyl residues with (1-6) ...19938319683
isomalto-oligosaccharide-containing lipoteichoic acid of streptococcus sanguis. microheterogeneity and distribution of chain substituents.the lipoteichoic acid of streptococcus sanguis dsm 20567 contains a poly(glycerophosphate) chain, with 49% of the glycerophosphate residues being substituted with d-alanine ester, 35% with alpha-d-glucopyranosyl and alpha-isomalto-oligosaccharide residues. analysis of molecular species by affinity chromatography on concanavalin a showed all chains to be substituted and alanine ester and glycosyl residues to be present on the same rather than on separate chains. molecular species varied in the le ...19938319684
[treatment with vancomycin of experimental endocarditis caused by streptococcus sanguis ii resistant to penicillin].streptococcus viridans continues to be the most frequent causal agent of infective endocarditis. treatment has become more complicated due to the increase in resistance to penicillin and cephalosporins. in order to study the possible efficacy of vancomycin, this antibiotic was investigated in rabbits as a monotherapy and in association with gentamicin. the effects were compared with a control group and a group given classical penicillin-gentamicin treatment.19938324022
in vitro antimicrobial activity of calcium hydroxide cements on streptococcus sanguis nctc 7864.several studies have questioned the antimicrobial role of calcium hydroxide cements. this study assessed the antimicrobial activity of four commercially available calcium hydroxide cements on streptococcus sanguis nctc 7864 using inoculated mueller-hinton growth medium. growth curves from optical density analysis, ph measurements, and viability counts were compared over a 24 h period. significant differences occurred (p < 0.05) between ph measurements but not the growth curves or the viability c ...19938330932
a critical appraisal of positive cooperativity in oral streptococcal adhesion: scatchard analyses of adhesion data versus analyses of the spatial arrangement of adhering bacteria.positive cooperativity is a mechanism proposed to account for the adhesion of bacteria to surfaces. in this paper, two methods that both claim to assess experimentally cooperative phenomena, viz. scatchard analysis of adhesion data (using adhesion to vials) and analysis of the spatial arrangement of adhering cells (using a flow chamber), were compared and critically evaluated. three oral strains were used and the substrata involved were glass (hydrophilic) and silicone-coated glass (hydrophobic) ...19938336110
mechanisms of platelet aggregation by streptococcus sanguis, a causative organism in infective endocarditis.the ability of certain strains of streptococcus sanguis to aggregate human platelets in vitro may be related to their virulence in the pathogenesis of infective endocarditis. we have studied the mechanisms of aggregation of human platelets by s. sanguis strain nctc 7863. platelet aggregation follows incubation of s. sanguis cells with platelet-rich plasma from normal, healthy adults, after a lag of 7-19 min. platelet aggregation was accompanied by 5-hydroxytryptamine release and thromboxane b2 p ...19938338784
differential expression of salivary mucin bacterial aggregating activity with caries status.1. the low and high mol. wt mucin forms were isolated from saliva of caries-resistant (cr) and caries-susceptible (cs) individuals, and assessed for their bacterial aggregating potential towards s. mutans and s. sanguis, the common cariogenic microorganisms encountered in the oral cavity. 2. the high mol. wt mucin from both groups of subjects exhibited similar protein and carbohydrate content, but the level of covalently bound fatty acids was significantly lower in the cr group. the mucin from c ...19938344449
characterization of allelic replacement in streptococcus parasanguis: transformation and homologous recombination in a 'nontransformable' streptococcus.we have obtained transformants of streptococcus parasanguis fw213 containing allelic replacements in several chromosomal loci. transformation occurred following electroporation with nonreplicating plasmids carrying two antibiotic-resistance-encoding genes, one of which is inserted into dna homologous to the chromosomal target. in contrast with other streptococci, s. parasanguis fw213 is not transformed by linear dna. mutations in nonreplicating plasmid dna preferentially replaced their homologue ...19938344531
transmission of mutans streptococci to infants following short term application of an iodine-naf solution to mothers' dentition.in a randomized clinical trial, six applications of an i2-naf or a placebo solution were administered to the mothers' dentition at the time of tooth emergence of her infant, to evaluate its effect on transmission of mutans streptococci (ms) to the infant. ms and other bacterial levels were periodically monitored in 48 mother-infant pairs until the child's third birthday. there were significant reductions in maternal salivary ms (p = 0.04), lactobacilli (p = 0.04), total streptococci (p = 0.002), ...19938348786
in vitro study of the penetration of three bacterial strains into root dentine.the purpose of this study was to assess in vitro migration of the following three bacterial species into dentinal tubules: streptococcus sanguis, actinomyces naeslundii and prevotella intermedia. twenty-seven bovine incisors were randomly divided into three groups. only the root dentin was used for the experiment. within each group, the nine incisors were sectioned into 36 dentin blocks that were incubated with one of the tested bacterial suspensions. twelve samples were taken after 10, 20, and ...19938351128
[a case of infective endocarditis in which a new vegetation appeared on a different site during chemotherapy].42-year-old man, who had been febrile for about a month, was admitted to our hospital. laboratory testing showed leukocytosis and high titer of crp. streptococcus sanguis ii was detected in his blood culture. according to the echocardiogram, he had a vegetation on the anterior mitral leaflet, so he was diagnosed as having infective endocarditis. antibiotic susceptibility test using the disc method showed (3+) response to penicillin g. after intravenous administration of 20 million units of penic ...19938351441
a simple method for monitoring mutans streptococci in margins of restorations.plaque material collected from margins of restorations using a sharp toothpick was streaked across one and the same plastic strip from a commercially available test kit and inoculated in a liquid medium containing bacitracin. the reproducibility of the method was good. the method is simple, inexpensive and suitable for the chairside demonstration of the colonization of mutans streptococci in localized areas of the mouth, such as margins of restorations.19938354746
bacteria reactive to plaque-toxin-neutralizing monoclonal antibodies are related to the severity of gingivitis at the sampled site.certain monoclonal antibodies (mabs) neutralize the inhibition of hl60 cell growth by bacterial plaque extracts. the aim of this study was to enumerate and identify the bacteria reactive to these mabs at sites where periodontal disease was measured by gingival index, pocket depth and attachment loss. the bacterial colonies were grown on blood agar and the colonies counted directly and after immunoblotting. sites that bled on probing (n = 15) had a mean of 3.35% mab-positive colonies (sem = 0.52% ...19938355987
unexpectedly high levels of some presumably protective secretory immunoglobulin a antibodies to dental plaque bacteria in salivas of both caries-resistant and caries-susceptible subjects.the role of salivary antibodies in protection against cariogenic bacteria is actually a matter of debate. correlation between caries experience and naturally occurring antibodies was extensively investigated. comparison of salivary antibodies from 21 caries-resistant and 22 caries-susceptible subjects was carried out by using a new quantitative method. secretory immunoglobulin a (s-iga) antibodies to streptococcus sobrinus and streptococcus sanguis cells were detected in all salivas and at simil ...19938359882
[a case of brain abscess due to streptococcus sanguis in association with multiple pulmonary arteriovenous fistulas].a case is reported here of brain abscess due to streptococcus sanguis in association with multiple pulmonary arteriovenous fistulas. a 19-year-old male who had been diagnosed in 1988 as pulmonary arteriovenous fistulas was admitted to our hospital for repeated epistaxis, headache, and vomiting. a brain abscess which was diagnosed using various roentogenologic examinations, worsened despite conservative therapy. additional surgical drainage was performed; s. sanguis was isolated from the drainage ...19938360524
cytotoxic and antibacterial effects of orthodontic appliances.the cytotoxic and antibacterial effects of orthodontic appliances were assessed. metallic devices used in orthodontics, such as molar bands, brackets, and archwires were tested by the agar overlay cytotoxicity test with mouse fibroblast cells. the same devices were tested for antibacterial effect with streptococcus mutans and s. sanguis. the multicomponent devices, which are bonded with silver- and copper-based brazing alloys, were more cytotoxic than the single-component devices, probably becau ...19938362202
identity of viridans streptococci isolated from cases of infective endocarditis.the oral streptococci have undergone considerable taxonomic revision in recent years but there is still little information concerning associations between the newly defined species and disease. this study examined the identities of 47 strains of oral streptococci collected from 42 confirmed cases of infective endocarditis. by means of recently described physiological schemes, the most common species identified were streptococcus sanguis sensu stricto (31.9%), s. oralis (29.8%) and s. gordonii (1 ...19938366515
effect of thrombocytopenia on the early course of streptococcal endocarditis.although platelets are a major factor in the pathogenesis of endocarditis, it is unclear if these cells promote or limit disease progression. to address this issue, the effects of thrombocytopenia on the early course of endovascular infection were examined. aortic valve endocarditis was produced in rabbits by using streptococcus sanguis m99. thrombocytopenia was induced by intravenous administration of antiplatelet serum. compared with controls (infected rabbits given nonimmune serum), thrombocy ...19938376837
the effects of triclosan and zinc citrate, alone and in combination, on a community of oral bacteria grown in vitro.a mixed-culture chemostat system has been used as a more stringent laboratory system for evaluation of the antimicrobial effects of triclosan and zinc citrate. the inhibitors were added alone, and in combination, as a pulse (a high initial inhibitor concentration which decreased with time) or as a dose (concentration of inhibitor increased with time) to give maximum concentrations of 34.5 mumol/l triclosan and 39.8 mumol/l zinc citrate. when dosed, triclosan inhibited a. viscosus and all five gr ...19938380287
nucleotide sequence analysis of the termini and chromosomal locus involved in site-specific integration of the streptococcal conjugative transposon tn5252.the 47-kb, broad-host-range, streptococcal conjugative transposon tn5252 is capable of site-specific integration into the pneumococcal chromosome. we present the nucleotide sequence of the terminal regions of the transposon and its target site in the pneumococcal genome. no inverted repeats were found at the termini of the transposon. a 72-bp region of the target was present on either side following the insertion of tn5252 and appeared to serve as a signal for its integration and excision. the d ...19938386725
the wall associated lipoteichoic acid of streptococcus sanguis.a competitive elisa is described for the measurement of lipoteichoic acid. the assay was used to determine the wall associated lipoteichoic acid of streptococcus sanguis which was found to represent only 2-4% of the phenol extractable content. extracellular lipoteichoic acid was detected even after exhaustive cell washing. this material was not the result of de novo synthesis because membrane de-polarization had no effect on the amount detected. since extracellular lipoteichoic acid interfered w ...19938386915
agglutination of streptococcus mutans serotype c cells but inhibition of porphyromonas gingivalis autoaggregation by human lactoferrin.the ability of various forms of human lactoferrin (lf) to agglutinate oral streptococcus mutans, strep. sobrinus, strep. rattus, strep. sanguis, porphyromonas gingivalis and actinobacillus actinomycetemcomitans cells was studied spectrophotometrically. fe3+ saturated lf was unable to agglutinate these bacteria, whereas iron-free lf (apo lf) effectively agglutinated strep. mutans cells but not the other bacteria. the efficiency and rate of agglutination of strep. mutans were somewhat lower with a ...19938387771
longitudinal study of relations between human salivary antimicrobial proteins and measures of dental plaque accumulation and composition.many studies have attempted to relate levels of antimicrobial proteins in saliva to oral health; results have been inconsistent, and one reason might be inconsistency of measures of plaque and saliva within subjects. this study investigated associations between plaque and salivary variables in longitudinal data. whole saliva, and 8-h plaque pooled from buccal first permanent molars, was obtained from 32 dental students on tuesdays from 3:00-6:00 p.m. over 4 weeks. salivary flow rate was determin ...19938392324
the comparative activity of azithromycin, macrolides and amoxycillin against streptococci in experimental infections.since serious sequelae may follow streptococcal infections, eradication is viewed as necessary for successful therapy. studies were therefore conducted to compare the effectiveness of azithromycin with other macrolide antibiotics and amoxycillin to eliminate these organisms in experimental localized infections. in a streptococcus pneumoniae lung infection induced by transtracheal challenge, the pathogen was not recovered after therapy with azithromycin (ed50 7.9 mg/kg), while clarithromycin was ...19938396094
involvement of alpha 2-adrenoreceptors and g proteins in the modulation of platelet secretion in response to streptococcus sanguis.in the presence of plasma, human platelets secrete the contents of their dense granules and then aggregate in response to certain strains of streptococcus sanguis. after 2 to 5 min of incubation with streptococci, platelets from fast-responding donors will begin to aggregate. slow responders aggregate after a longer delay. platelets may secrete after a short (fast responder) or long (slow responder) delay because of differences in the basal levels or responses to potentiating catecholamines. to ...19938397001
purification and characterization of chimeric human iga1 and iga2 expressed in cos and chinese hamster ovary cells.ag-specific chimeric human iga molecules, of the two human subclasses, iga1 and iga2, have been expressed in two mammalian cell systems. analysis of the secreted iga molecules, purified in milligram quantities from stable chinese hamster ovary transfectants by ag affinity chromatography, has allowed a direct comparison of the biologic properties of the two subclasses. hplc gel filtration analysis revealed that in both subclasses, the iga molecules associate predominantly into dimers. the monomer ...19938409433
distribution and isolation frequency of eight streptococcal species in saliva from predentate and dentate children and adults.the isolation frequency and distribution of eight recently defined streptococcal species have been investigated in the saliva of adults and that of both predentate and dentate children. the effects of frequency of sugar intake and tooth presence on the distribution of streptococcal species were also analyzed. saliva samples were collected from 121 subjects divided into three study groups: (a) 56 predentate children (predentate group), (b) 37 dentate children (dentate group), and (c) 28 adults (a ...19938418104
saliva-binding protein (ssab) from streptococcus sanguis 12 is a lipoprotein.two lipoprotein consensus sequences (leu-x-x-cys) are found in the presumptive signal peptide region (positions 12 to 15 and 17 to 20) of saliva-binding protein (ssab) from streptococcus sanguis 12. three analogs of ssab containing cys-->gly mutations were constructed by site-directed mutagenesis of psa2, the recombinant plasmid expressing ssab. [3h]palmitate was incorporated into ssab only when the native cys-20 residue was present. these data show that ssab is a lipoprotein and that cys-20 is ...19938419308
home antibiotic therapy for streptococcal endocarditis: a call for a controlled trial. 19938420289
the streptococcus sanguis platelet aggregation-associated protein. identification and characterization of the minimal platelet-interactive domain.streptococcus sanguis expresses a cell wall-bound protein that induces the activation and aggregation of platelets. this platelet aggregation-associated protein (paap) contains a collagen-like, platelet-interactive domain within a 23-kda protein fragment. to isolate the minimal platelet-interactive domain, p23 paap was digested with collagenase, and the digest chromatographed to isolate fractions with activity inhibitory to s. sanguis-induced platelet aggregation. the active fraction was then di ...19938420939
the dental status and attitudes of patients at risk from infective endocarditis.infective endocarditis represents one of the few potentially fatal infections that may be caused in the patient by a dentist. efforts to reduce the incidence of this disease usually take the form of appropriate antibiotic cover prior to dental treatment, together with the establishment and maintenance of good oral health. this paper is a report of a survey on the dental health and awareness of 81 'at-risk' patients attending a cardiology outpatient clinic. it was found that whilst the patients w ...19938422317
acute valvular obstruction from streptococcal endocarditis. 19938427158
interfering alpha-streptococci as a protection against recurrent streptococcal tonsillitis in children.recurrent streptococcal tonsillitis/pharyngitis is a great problem, especially in certain epidemiological situations. patients treated with antibiotics often have a disturbed normal throat flora and may lack, e.g., alpha-streptococci known in vitro to have an interfering activity against group a streptococci. thirty-one patients with recurrent streptococcal tonsillitis were given antibiotics for 10 days. at the end of this treatment they were sprayed in their mouths with four selected alpha-stre ...19938436458
biosynthesis and characterization of phosphatidylglycerophosphoglycerol, a possible intermediate in lipoteichoic acid biosynthesis in streptococcus sanguis.a membrane enzyme preparation from streptococcus sanguis was shown to convert sn-[14c]glycerol 3-phosphate and cdp-diacylglycerol (or deoxycdp-diacylglycerol) into a series of progressively higher-molecular-weight [14c]oligophosphoglycerophospholipids in vitro. the first oligophosphoglycerophospholipid to accumulate (termed lipid-1) was purified to homogeneity; chemical analysis, gas-liquid chromatography and chemical degradation studies indicated the most likely structure to be phosphatidylglyc ...19938443240
mixed continuous cultures of streptococcus mutans with streptococcus sanguis or with streptococcus oralis as a model to study the ecological effects of the lactoperoxidase system.mixed continuous cultures of streptococcus species were obtained, using complex carbohydrate (mucin) as a source of nutrients, to study the ecological effects of oxygen and the lactoperoxidase system. s. mutans nctc 10449 was unable to grow as a pure culture on mucin, but attained a significant population size in the presence of s. oralis and s. sanguis strains. the cell densities of the anaerobic mixed cultures decreased when oxygen was supplied, and s. mutans was more suppressed by oxygen than ...19938448770
ph changes in human dental plaque from lactose and milk before and after adaptation.ph changes in human dental plaque in vivo from lactose and milk have been studied in four separate mouthrinse experiments. (1) ph was measured in plaque samples of 12 subjects before and after 6 weeks of frequent daily mouthrinsing with 10% lactose. the ph decreases were significantly more pronounced after the adaptation period than before (p < 0.001), 4 of the 12 subjects showing about the same low plaque ph from lactose as from glucose. (2) similar to the previous experiment, frequent daily mo ...19938448773
synergistic activity of ceftriaxone combined with netilmicin administered once daily for treatment of experimental streptococcal endocarditis.we have conducted experiments to determine if one daily injection of netilmicin (net) would be synergistic with the broad-spectrum cephalosporin ceftriaxone (cro) in the treatment of experimentally induced endocarditis. rats with catheter-induced aortic vegetations were infected intravenously with 3 x 10(7) cfu of a beta-lactam-sensitive strain of streptococcus sanguis or a beta-lactam-resistant strain of streptococcus mitis. treatment with the antibiotics alone (cro, 10 mg/kg of body weight eve ...19938452350
infective endocarditis after transesophageal echocardiography. 19938462170
coaggregation: specific adherence among human oral plaque bacteria.nearly all human oral bacteria exhibit coaggregation, cell-to-cell recognition of genetically distinct cell types. clumps or coaggregates composed of the two kinds of cells are formed immediately upon mixing two partner cell types. members of all 18 genera tested exhibit lactose-reversible coaggregation. many of these interactions appear to be mediated by a lectin on one cell type that interacts with a complementary carbohydrate receptor on the other cell type. a lactose-sensitive adhesin has be ...19938462782
determination by heteronuclear nmr spectroscopy of the complete structure of the cell wall polysaccharide of streptococcus sanguis strain k103.although complete structures of complex polysaccharides have traditionally been determined by chemical degradative methods, a number of recent developments in instrumentation have greatly facilitated this task. we illustrate the application of several of these methods in a determination of the complete covalent structure of the polysaccharide from streptococcus sanguis k103, which is composed of an octasaccharide repeating subunit linked by phosphodiester bonds. carbohydrate analysis by hpae-pad ...19938470820
infectious crystalline keratopathy. the role of bacterial exopolysaccharide.infectious crystalline keratopathy is a unique pauci-inflammatory infection of the cornea most commonly due to viridans type streptococci. we investigated the hypothesis that production of exopolysaccharide by streptococci, a property that can be induced by growth conditions, may contribute to the pathogenesis of infectious crystalline keratopathy by suppressing the ocular immune response.19938470987
targeting and delivery of bactericide to adsorbed oral bacteria by use of proteoliposomes.proteoliposomes having surface-bound succinylated concanavalin a (s-cona) have been prepared from a range of phospholipid mixtures by sonication (suv) and reverse phase evaporation (rev) covering a range of size (weight-average diameter (dw)) from approx. 35 to 310 nm and weight-average number of protein molecules per liposomes (pw) from approx. 50 to 3000. the targeting of the proteoliposomes to adsorbed biofilms of the bacteria streptococcus sanguis and streptococcus mutans has been assessed f ...19938476919
role of superantigens in human disease.superantigens include bacterial products (mainly of streptococci and staphylococci) that stimulate t cells to proliferate nonspecifically through interaction with class ii major histocompatibility complex products on antigen-presenting cells and then with variable regions on the beta chain of the t cell receptor complex. they include pyrogenic toxins (streptococcal scarlet fever toxins of serotypes a, b, and c, toxic shock syndrome toxin 1, and staphylococcal enterotoxin serotypes a, b, cn, d, e ...19938486972
defence case known as 'heart'. 19938494514
formation of alpha-(1-->6), alpha-(1-->3), and alpha-(1-->2) glycosidic linkages by dextransucrase from streptococcus sanguis in acceptor-dependent reactions.dextransucrase from streptococcus sanguis 10558 was found to synthesize alpha-(1-->6), alpha-(1-->3), and alpha-(1-->2) linkages during an acceptor-dependent glucosyl transfer reaction. normally, new glucosyl residues are added at c-6 of monosaccharide acceptors. however, sugars blocked at c-6 also can serve as good acceptors. the disaccharide and trisaccharide products formed when methyl 6-bromo-6-deoxy-alpha-d-glucopyranoside was used as acceptor were isolated and characterized. both were foun ...19938495440
xylitol and the bactericidal effect of chlorhexidine and fluoride on streptococcus mutans and streptococcus sanguis.the present study was made to investigate the effect of xylitol on the bactericidal and bacteriostatic action of chlorohexidine diacetate (chx) and sodium fluoride (f) in atcc strains of streptococcus mutans and s. sanguis. standardized bacterial cell suspensions were used in tests for bactericidal effect and for inhibition of growth and sucrose fermentation. the results showed no interference of xylitol with the antibacterial effect of chx and f combinations. xylitol did not show any additive e ...19938498160
oral streptococcal colonization of infants.the objective of this study was to determine the prevalence and proportions of different streptococcal species among the streptococcal flora during infancy. a total of 60 oral samples were collected by oral swabbing of the buccal mucosa and alveolar ridges of 18 infants before tooth eruption and from buccal and lingual surfaces of teeth after tooth eruption. a total of 549 isolates on mitis salivarius agar were speciated, principally by recently revised biochemical criteria of kilian et al. stre ...19938510978
dental plaque development on defined streptococcal surfaces.coaggregations between bacterial species have been widely studied in vitro but not in the mouth. a new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. chips were covered with streptococcus oralis atcc 10557, which coaggregated with many oral species, or streptococcus gordonii s7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. there were no di ...19938510979
an analysis of the subgingival microflora in randomly selected subjects.the aim of this study was to describe the presence of some microbial species in the subgingival microflora of a randomly selected subject sample. a further objective was to analyze the association between some microbiological characteristics and the periodontal conditions of the subjects. a total of 171 individuals aged 30-65 years were included. a pooled subgingival plaque sample was obtained from the mesial aspect of the 6 ramfjord teeth by the use of the paper-point method. the samples were p ...19938510980
acid production by human strains of streptococcus mutans and streptococcus sobrinus.acid production by washed suspensions of human strains of streptococcus mutans (n = 18) and streptococcus sobrinus (n = 12) was measured. the strains were isolated from infants and adolescents with varying caries experience. some of these strains and two laboratory strains (1 s. mutans and 1 s. sobrinus) had been tested in an earlier study for their cariogenicity in hamsters. further, 3 streptococcus sanguis strains and 1 s. sobrinus laboratory strain were included. acid production was determine ...19958521443
detection of binding of denatured salivary alpha-amylase to streptococcus sanguis.native alpha-amylase, either solubilized, or immobilized and tested with an overlay immunotechnique, was bound in a species-specific manner to streptococcus mitis and to one of the streptococcus gordonii strains. however, only insignificant amounts of alpha-amylase were bound to streptococcus sanguis and all other strains tested. when alpha-amylase was denatured before immobilization, streptococcus sanguis bound strongly to the protein while binding of other strains was insignificant.19958526808
arginine deiminase system and acid adaptation of oral streptococci.streptococcus rattus fa-1 and streptococcus sanguis nctc 10904 underwent phenotypic acid adaptation in batch cultures toward the end of sugar-fueled growth after the culture ph had dropped to triggering values. the bacteria could be derepressed or induced for arginine deiminase independently of acid adaptation, and arginolysis afforded protection against acid killing over and above that of acid adaptation.19958534118
influence of monocytes and antibiotic treatment on tissue factor activity of endocardial vegetations in rabbits infected with streptococcus sanguis.a main feature in the pathogenesis of bacterial endocarditis is the activation of the coagulation system via the extrinsic pathway, resulting in the formation of infected endocardial vegetations. earlier studies gave indirect evidence that monocytes play an important role in the procoagulant response during the course of the disease. in this study, we assessed the role of monocytes more directly. we compared weights and tissue factor activities (tfa) of endocardial vegetations of normal rabbits ...19968550190
prb1, prb2, and prb4 coded polymorphisms among human salivary concanavalin-a binding, ii-1, and po proline-rich proteins.six closely linked prp (proline-rich protein) genes code for many salivary prps that show frequent length and null variants. from determined protein sequences and dna sequence analysis of variant alleles, we here report the coding and molecular basis for con (concanavalin a-binding) and po (parotid "o") protein polymorphisms. the con1 glycoprotein is encoded in exon 3 of a prb2 allele (prb2l con1+) with a potential n-linked glycosylation site. because of a probable gene conversion encompassing > ...19968554050
adherence of microorganisms to rat salivary pellicles.numerous studies, using models to mimic the formation of the acquired pellicle, have concentrated on human saliva-coated hydroxyapatite; in contrast, although the rat is frequently used as an animal model, the information concerning the formation of pellicles from rat saliva is sparse. we compared the ability of streptococcus and actinomyces species to adhere to hydroxyapatite coated by rat saliva (rsha) and human saliva (sha). we also explored the influence of glucan synthesized in situ on the ...19958556756
viridans streptococci isolated from the bloodstream. relevance of species identification.over a 4-year-period, 104 isolates belonging to the viridans streptococci were recovered from the blood and identified to the species according to beighton and co-workers. streptococcus oralis was the species most frequently recovered from patients in the hematology unit [29 of 39 (74%)]. streptococcus mitis ranked second [seven of 39 (18%)]. both species were associated with oromucositis. isolates presently identified as s. oralis are mainly those previously identified as s. mitis or streptococ ...19958565415
degradation and fermentation of fructo-oligosaccharides by oral streptococci.fructo-oligosaccharides (fos) are claimed to have a positive effect on the intestinal flora. they are being used in functional foods in japan and europe. this group have tested the degradation of two commercial fos preparations by oral streptococci in order to predict the cariogenicity of these products. both preparations could be fermented to some extent by the species of oral streptococci tested. the enzymes necessary for the degradation of fos were inducible. each strain showed a specific deg ...19958567492
adherence, accumulation, and cell division of a natural adherent bacterial population.developing dental bacterial plaques formed in vivo on enamel surfaces were examined in specimens from 18 adult volunteers during the first day of plaque formation. an intraoral model placing enamel pieces onto teeth was used to study bacterial plaque populations developing naturally to various cell densities per square millimeter of surface area of the enamel (w. f. liljemark, c. g. bloomquist, c. l. bandt, b. l. philstrom, j. e. hinrichs, and l. f. wolff, oral microbiol. immunol. 8:5-15, 1993). ...19968576054
characterization of streptococcus sanguis isolated from patients with behçet's disease.the dna homology and cell wall sugar constituents of eight streptococcus sanguis(-like) strains, three isolated from the patients with behçet's disease (bd114-23, bd113-20, bd118-1), two from patients with kawasaki disease (mcls-1, mcls-2), and three type and reference strains of atcc (atcc10556t: s. sanguis, atcc10557: s. oralis, and atcc10558t: s. gordonii) were analyzed. strains bd114-23 and bd118-1 showed high dna homology to atcc10556t, and their cell wall constituents were identical. conve ...19958577288
cross-reactivity between human sialyl lewis(x) oligosaccharide and common causative oral bacteria of infective endocarditis.the expression of sialy-lewis(x) (sle(x); neu5ac alpha 2-3 gal beta 1-4) (fuc alpha 1-3) glcnac-r) on oral bacteria producing infective endocarditis was determined by a whole-cell enzyme linked immunosorbent assay and an immunoelectron microscopy using the well-characterized anti-sle(x) monoclonal antibody snh-3 (mab snh-3; igm class). mab snh-3 reacted strongly with whole cells of oral bacteria: streptococcus sanguis, streptococcus mutans, streptococcus mitis, streptococcus salivarius, streptoc ...19958589666
enhanced activity of the combination of penicillin g and gentamicin against penicillin-resistant viridans group streptococci.we evaluated the effects of the combination of penicillin g and gentamicin against 10 penicillin-resistant bacteremic isolates of viridans group streptococci for which the mics of penicillin were 4 to 64 micrograms/ml. in time-kill studies, the combination resulted in more killing of eight isolates for which the mics of penicillin were from 8 to 64 micrograms/ml than any of the antimicrobial agents tested alone. in general, clearly enhanced antimicrobial activity was observed with the combinatio ...19958593029
insertional inactivation of binding determinants of streptococcus crista cc5a using tn916.intermicrobial binding plays an important role in the ecology of the oral cavity because it represents one mechanism by which specific bacteria colonize dental plaque. the formation of "corncobs", a morphologically distinct microbial unit composed of streptococcus crista and fusobacterium nucleatum, is a highly specific binding interaction that depends on the presence of polar tufts of fimbriae on the streptococci. we have used a genetic approach to examine the role of streptococcal cell surface ...19958602334
modulation of glycosidase and protease activities by chemostat growth conditions in an endocarditis strain of streptococcus sanguis.the effects of growth conditions on the properties of the endocarditis-producing oral bacterium streptococcus sanguis fss2 were studied. this strain produces a variety of proteases and glycosidases, including a thrombin-like activity that is a potential virulence factor for endocarditis. cultures were grown with limiting glucose or galactose in chemostats over a range of dilution rates and ph levels, and the following activities were measured at ph 7.5: thrombin-like, hageman factor-like, n-acet ...19958602341
simultaneous monitoring of intracellular ph and proton excretion during glycolysis by streptococcus mutans and streptococcus sanguis: effect of low ph and fluoride.a system was developed by which 2',7'-bis(carboxyethyl)-4 or 5-carboxyfluorescein could be used to monitor intracellular ph at the same time that proton excretion was being measured. streptococcal cells were loaded with the dye, and after the addition of glucose protons were excreted and the intracellular ph increased quickly and remained higher than the extracellular ph of 7.0. the excretion of protons stopped and the intracellular ph returned to the original level when glucose was depleted. th ...19958602343
lipoteichoic acid and interleukin 1 stimulate synergistically production of hepatocyte growth factor (scatter factor) in human gingival fibroblasts in culture.lipoteichoic acids (lta) from various gram-positive bacteria, including oral streptococci such as streptococcus sanguis, enhanced the production of hepatocyte growth factor (hgf) (scatter factor) by human gingival fibroblasts in culture, whereas lipopolysaccharides (lps) from various gram-negative bacteria did not. in contrast, lps induced interleukin 1 activity in human gingival epithelial cells in culture, while lta had little effect. lta and recombinant human interleukin 1 alpha enhanced syne ...19968606111
studies into the microbial spectrum of apical periodontitis.this study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. a total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. bacteria were identified by applying the following techniques: culturing on various media, gram-staining and using commercially available biochemical test strips. in addition, prevotella intermedia and porphyromonas endodontalis were d ...19958626206
overproduction and characterization of the stsi restriction endonuclease.the stsi restriction endonuclease (r-stsi), a class-iis restriction endonuclease, found in streptococcus sanguis 54, is a heteroschizomer of r-foki, which recognizes 5'-ggatg-3'. to overproduce r-stsi in escherichia coli, the coding region of r-stsi was joined to the tac promoter of an expression vector, pkk223-3. by introduction of the plasmid into e. coli ut481 cells expressing the fokim gene, r-stsi activity was overproduced, from which r-stsi was purified homogeneously. we compared the prope ...19968635752
a host-vector system for heterologous gene expression in streptococcus gordonii.we have developed a host-vector system for heterologous expression in streptococcus gordonii (sg) challis (formerly streptococcus sanguis), a commensal bacterium of the human oral cavity. the system is based on (i) integration of plasmid insertion vectors into the chromosome of specially engineered recipient hosts, and (ii) the use of the m6-protein-encoding gene (emm6) as a partner for construction of translational gene fusions. m6 is a streptococcal surface protein already proven useful as a f ...19968635755
resistance of streptococcus sanguis biofilms to antimicrobial agents.bacteria living in biofilms as dental plaque on tooth surfaces are generally more resistant to antimicrobial agents than bacteria in batch culture normally used for in vitro susceptibility testing. in order to compare the resistance of free-living and surface-grown oral bacteria, the mic of streptococcus sanguis 804 and atcc 10556 to amoxicillin, doxycycline and chlorhexidine was determined by a broth dilution method. subsequently, s. sanguis biofilms established in an in vitro flow model were p ...19968645467
co-adhesion of oral microbial pairs under flow in the presence of saliva and lactose.co-aggregation (interactions between two suspended micro-organisms) between oral microbial pairs has been studied extensively and is believed to be an important factor in dental plaque formation. however, co-adhesion (interactions between suspended and already-adhering micro-organisms) may well be equally important. the aim of this paper was to determine the influence of saliva and lactose on the co-adhesion of streptococci (s. oralis 34 and s. sanguis pk1889) to actinomyces (a. naeslundii t14v- ...19968655779
the inhibiting effect of aqueous azadirachta indica (neem) extract upon bacterial properties influencing in vitro plaque formation.the purpose of this investigation was to examine the inhibitory effects of aqueous extracts derived from the bark-containing sticks (neem stick) of azadirachta indica upon bacterial aggregation, growth, adhesion to hydroxyapatite, and production of insoluble glucan, which may affect in vitro plaque formation. neem stick extracts were screened for minimal bacterial growth inhibition (mic) against a panel of streptococci by means of a broth dilution assay. initial bacterial attachment was quantifi ...19968655780
identification and classification of species within the streptococcus sanguis group.a biochemical identification scheme has been produced that allows for the differentiation of nine distinct species within the streptococcus sanguis group. the species are s. sanguis, s. gordonii, s. crista, s. oralis, s. parasanguis, s. defectivus, s. adjacens and s. mitis, which have been previously described, and a new species, s. australis; in addition two distinct subspecies, s. oralis subsp. corona and s. oralis subsp. mitior, have been identified. dna-dna hybridization confirmed the separa ...19968670030
a method for evaluating the cariogenicity of oral bacteria using radio-labelled synthetic hydroxyapatite.radioactive hydroxyapatite was synthesized using 45ca to evaluate the cariogenicity of oral streptococci. discs prepared from it were suspended in media containing sucrose, then inoculated with streptococcus mutans or streptococcus sanguis. the radioactivity in the supernatant fluid was measured at specified time intervals. released 45ca in the supernatant fluids markedly increased in both species during the experimental period, while strep. mutans showed much higher decalcification than strep. ...19968672277
evaluation of the automicrobic system for the identification of streptococcus mutans.the performance of the automicrobic system with the vitek gram-positive identification card (biomérieux, france) in identifying strains of streptococcus mutans was studied. of 160 strains assayed, 72.5% were confirmed to be streptococcus mutans; the remainder were identified as other species of streptocci (streptococcus bovis, streptococcus uberis, streptococcus anginosus, streptococcus sanguis i and ii, streptococcus intermedius, and streptococcus constellatus).19958681990
eliminating effects of an air purifier on infectants during dental procedure.blood and drill dust from dental plaque microorganisms, teeth, and filling materials can cause environmental pollution in the dental clinic. currently, as a preventive measure against air pollution from a patient's mouth during dental treatment, dust-collecting aspirators such as an extra-oral vacuum aspirator (eova) are coming into general use. we tested the eliminating effects by the eova with the plaque solution aerosol and the aerosol from drilling a tooth by examining the distribution of fl ...19958689739
identification and characterization of the genes of enterococcus faecalis plasmid pcf10 involved in replication and in negative control of pheromone-inducible conjugation.the prgb gene of the enterococcus faecalis pheromone-inducible conjugative plasmid pcf10 encodes the surface protein asc10. this protein mediates cell aggregation and its expression results in high-frequency transfer of the plasmid from donor to recipient. to identify the minimum region necessary for negative regulation of prgb expression, target plasmids were constructed containing a recently identified positive control region and a prgb::lacz transcriptional fusion; expression of prgb in cells ...19968693026
endophthalmitis after filtering surgery with mitomycin.to identify the incidence, causative organisms, and clinical outcomes of eyes with bleb-associated endophthalmitis after glaucoma filtering procedures with adjunctive mitomycin.19968694729
enhanced bactericidal activity of arm and hammer dental care.to compare and contrast antibacterial activities of a baking soda-containing dentifrice, arm and hammer dental care (ahdc) with two fluoride dentifrices without baking soda (crest and colgate).19958695008
oral bacterial attachment to titanium surfaces: a scanning electron microscopy study.despite the wide use of dental implants, the understanding of the mechanism(s) of bacterial attachment to implant surfaces and of the factors that affect such attachment is limited. in this study, the attachment of oral bacteria--including streptococcus sanguis, actinomyces viscosus, and porphyromonas gingivalis--to titanium (ti) discs with different surface morphology (smooth, grooved, or rough) was examined by scanning electron microscopy (sem). the most bacterial attachment was observed on th ...19958699514
the effect of oral commensal bacteria on candidal adhesion to denture acrylic surfaces. an in vitro study.the effect of four different species of oral bacteria (streptococci (x 2); escherichia; porphyromonas) on the adhesion of candida albicans and candida krusei to denture acrylic surfaces was studied using a previously described in vitro adhesion assay. clear acrylic strips immersed in known concentrations of bacterial suspensions and incubated for 45 min to 1 h, at 37 degrees c, were transferred to yeast suspensions of known concentrations and incubated for a similar period, and the adherent yeas ...19968703439
killing of streptococcus sanguis in biofilms using a light-activated antimicrobial agent.the aim of this study was to determine whether streptococcus sanguis, when in a biofilm, could be killed using a light-activated antimicrobial agent. biofilms were grown on hydroxyapatite, irradiated with up to 12.2 j of light from a gallium aluminium arsenide laser in the presence of aluminium disulphonated phthalocyanine (alpcs2) and survivors enumerated. no significant decrease in the viable count was found when either the alpcs2 or the laser light was used alone. there was a light dose relat ...19968707751
purification of nadh: hypothiocyanite oxidoreductase in streptococcus sanguis.nadh: hypothiocyanite oxidoreductase (nhor) activity, found in some oral streptococci, is postulated to protect these microorganisms against salivary peroxidase-produced hypothiocyanite. nhor, however, has not been purified so far. the purification of nhor from crude extracts of streptococcus sanguis nctc 7863 strain (by ultrafiltration and anion-exchange chromatography) revealed one fraction of 125 +/- kda. however, sds-page electrophoresis provided a single protein of 21.1 +/- 1.2 kda. this la ...19968733891
some effects of fluoride on the iga protease of the oral bacterium streptococcus sanguis. 19968736970
effect of titanium on selected oral bacterial species in vitro.titanium granules were tested for their antibacterial effect on strains of streptococcus sanguis, streptococcus mitis, actinomyces naeslundii, haemophilus parainfluenzae, fusobacterium spp. and prevotella intermedia in comparison with amalgam and two of its components, copper and tin. glass beads were used as controls. the number of viable bacteria was estimated in samples exposed to the various materials for 1, 3, 6 and 24 h, respectively, and the viable counts were related to the baseline valu ...19958747674
influence of lipoteichoic acid structure on recognition by the macrophage scavenger receptor.lipoteichoic acids (ltas) belong to the immunostimulatory class of molecules of gram-positive bacteria (gpb). previous investigations showed that the macrophage scavenger receptor (sr), a glycosylated trimeric transmembrane protein, binds directly to many gpb, possibly via lta. sr binding to other ligands is dependent upon the spatial characteristics of the repeating negative charge of the ligand. we therefore investigated sr recognition of lta species with various charge densities and distribut ...19968757870
[adherent mechanism of the mutans of actinomyces viscosus t14v].three mutans of actionmyces viscous t14v lacking type1 (5951), type 2 (5519) and both types of fimbriae (147) were selected in this study. we compared the ability of their adsorption to the test-tube wall, the result of the cellular coaggregation between these mutant strains and streptococcus sanguis 34, streptococcus sanguis black bova, streptococcus mutans ingbritte, streptococcus sobrinus 6715 and analysed the chemical characterization of cell walls of the three mutans. we found that the spec ...19958762533
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