Publications

The acid-tolerant L-arabinose isomerase from the mesophilic Shewanella sp. ANA-3 is highly active at low temperatures.	ABSTRACT:	2011	22074172
identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis candidates.	to describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ...	2012	22074685
enhanced performance and mechanism study of microbial electrolysis cells using fe nanoparticle-decorated anodes.	anode properties are critical for the performance of microbial electrolysis cells (mecs). in the present study, fe nanoparticle-modified graphite disks were used as anodes to investigate the effects of nanoparticles on the performance of shewanella oneidensis mr-1 in mecs. results demonstrated that the average current densities produced with fe nanoparticle-decorated anodes up to 5.89-fold higher than plain graphite anodes. whole genome microarray analysis of the gene expression showed that gene ...	2011	22080340
E622, a miniature, virulence-associated mobile element.	Miniature inverted terminal repeat elements (MITEs) are nonautonomous mobile elements that have a significant impact on bacterial evolution. Here we characterize E622, a 611-bp virulence-associated MITE from Pseudomonas syringae, which contains no coding region but has almost perfect 168-bp inverted repeats. Using an antibiotic coupling assay, we show that E622 is transposable and can mobilize an antibiotic resistance gene contained between its borders. Its predicted parent element, designated T ...	2012	22081398
the metj regulon in gammaproteobacteria determined by comparative genomics methods.	whole-genome sequencing of bacteria has proceeded at an exponential pace but annotation validation has lagged behind. for instance, the metj regulon, which controls methionine biosynthesis and transport, has been studied almost exclusively in e. coli and salmonella, but homologs of metj exist in a variety of other species. these include some that are pathogenic (e.g. yersinia) and some that are important for environmental remediation (e.g. shewanella) but many of which have not been extensively ...	2011	22082356
Roles of siderophore in manganese-oxide reduction by Shewanella oneidensis MR-1.	Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study constructed a random transposon-insertion mutant library of MR-1 and screened it for isolating mutants that were deficient in metal reduction. Examination of approximately 5000 mutant ...	2012	22092340
High frequency of glucose-utilizing mutants in Shewanella oneidensis MR-1.	Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however suggests the ability to use glucose. Here, we demonstrate that during initial glucose exposure, S. oneidensis MR-1 quickly and frequently gains the ability to utilize glucose as a sole carbon source, in contrast to wild-type S. oneidensis, which cannot immediately use glucose as a sole carbon substrate. High-performance liquid chr ...	2011	22092702
Detection of biological uranium reduction using magnetic resonance.	The conversion of soluble uranyl ions (UO?22+) by bacterial reduction to sparingly soluble uraninite (UO(2(s)) ) is being studied as a way of immobilizing subsurface uranium contamination. Under anaerobic conditions, several known types of bacteria including iron and sulfate reducing bacteria have been shown to reduce U (VI) to U (IV). Experiments using a suspension of uraninite (UO(2(s)) ) particles produced by Shewanella putrefaciens CN32 bacteria show a dependence of both longitudinal (T(1) ) ...	2011	22095467
Recurrent Ochrobactrum anthropi and Shewanella putrefaciens bloodstream infection complicating hemodialysis.	Bloodstream infections (BSIs) are common in hemodialysis, especially when the access is a catheter. These infections are more commonly gram-positive bacteria or gram-negative bacilli and on some occasions, fungi. Ochrobactrum anthropi and Shewanella putrefaciens are ubiquitous hydrophilic gram-negative bacilli. There have been three cases of O.?anthropi BSI reported in hemodialysis patients (one from the United States and two from Vienna) and two cases of S.?putrefaciens BSI in hemodialysis pati ...	2011	22099546
resin-based investigation of acyl carrier protein interaction networks in escherichia coli.	protein-protein interactions play an integral role in metabolic regulation. elucidation of these networks is complicated by the changing identity of the proteins themselves. here we demonstrate a resin-based technique that leverages the unique tools for acyl carrier protein (acp) modification with non-hydrolyzable linkages. acps from escherichia coli and shewanella oneidensis mr-1 are bound to affigel-15 with varying acyl groups attached and introduced to proteomic samples. isolation of these bi ...	2011	22104437
Fabrication of macroporous chitosan scaffolds doped with carbon nanotubes and their characterization in microbial fuel cell operation.	Chitosan (CHIT) scaffolds doped with multi-walled carbon nanotubes (CNT) were fabricated and evaluated for their utility as a microbial fuel cell (MFC) anodic material. High resolution microscopy verified the ability of Shewanella oneidensis MR-1 to directly colonize CHIT-CNT scaffolds. Cross-linking agents 1-ethyl-3-[3-dimethylaminopropyl] carbodimide hydrochloride (EDC), glutaraldehyde and glyoxal were independently studied for their ability to strengthen the CHIT-CNT matrix without disrupting ...	2011	22113017
isolation of shewanella algae from rectal swabs of patients with bloody diarrhoea.	shewanella algae is an emerging bacteria rarely implicated as a human pathogen. it was infrequently recovered from clinical specimens probably because of inadequate processing of non-fermenting oxidase-positive gram-negative bacilli. we report here isolation of s. algae in pure culture and mixed with e. coli from two cases of acute gastroenteritis with bloody mucous containing diarrhea occurring at the same time. as this organism is not a normal flora of the gut, the possible source of infection ...	2011	22120808
Comparative bioelectricity production from various wastewaters in microbial fuel cells using mixed cultures and a pure strain of Shewanella oneidensis.	Current and power density from four wastewaters, agriculture (AWW), domestic (DWW), paper (PWW), and food/dairy (FDWW), were comparatively evaluated in combination with three inocula: wastewater endogenous microbes (MFC1), Shewanella oneidensis MR-1 (MFC2), and wastewater endogenous microbes with MR-1 (MFC3) in single chamber microbial fuel cells (MFC). Using AWW (0.011mA/cm(2); 0.0013mW/cm(2)) and DWW (0.017mA/cm(2); 0.0036mW/cm(2)), MFC2 was the best candidate providing the maximum current, wh ...	2012	22123299
identification of novel sweet protein for nutritional applications.	the prevalence of obesity and diabetes has increased exponentially in recent years around the globe, especially in india. sweet proteins have the potential to substitute the sugars, by acting as natural, good and low calorie sweeteners. they also do not trigger a demand for insulin in diabetic patients unlike sucrose. in humans, the sweet taste perception is mainly due to taste-specific g protein-coupled heterodimeric receptors t1r2-t1r3. these receptors recognize diverse natural and synthetic s ...	2011	22125379
evidence-based annotation of gene function in shewanella oneidensis mr-1 using genome-wide fitness profiling across 121 conditions.	most genes in bacteria are experimentally uncharacterized and cannot be annotated with a specific function. given the great diversity of bacteria and the ease of genome sequencing, high-throughput approaches to identify gene function experimentally are needed. here, we use pools of tagged transposon mutants in the metal-reducing bacterium shewanella oneidensis mr-1 to probe the mutant fitness of 3,355 genes in 121 diverse conditions including different growth substrates, alternative electron acc ...	2011	22125499
The Response of Shewanella oneidensis MR-1 to Cr(III) Toxicity Differs from that to Cr(VI).	Chromium is a contaminant of concern that is found in drinking water in its soluble, hexavalent form [Cr(VI)] and that is known to be toxic to eukaryotes and prokaryotes. Trivalent chromium [Cr(III)] is thought to be largely harmless due to its low solubility and inability to enter cells. Previous work has suggested that Cr(III) may also be toxic to microorganisms but the mechanism remained elusive. In this work, we probe the toxicity of Cr(III) to Shewanella oneidensis MR-1, a bacterium able to ...	2011	22125549
specificity of motor components in the dual flagellar system of shewanella putrefaciens cn-32.	bacterial flagellar motors are intricate nanomachines in which the stator units and rotor component flim may be dynamically exchanged during function. similar to other bacterial species, the gammaproteobacterium shewanella putrefaciens cn-32 possesses a complete secondary flagellar system along with a corresponding stator unit. expression of the secondary system occurs during planktonic growth in complex media and leads to the formation of a subpopulation with one or more additional flagella at ...	2011	22151089
Aggrandizing power output from Shewanella oneidensis MR-1 microbial fuel cells using calcium chloride.	There are several interconnected metabolic pathways in bacteria essential for the conversion of carbon electron sources directly into electrical currents using microbial fuel cells (MFCs). This study establishes a direct exogenous method to increase power output from a Shewanella oneidensis MR-1 containing MFC by adding calcium chloride to the culture medium. The current output from each CaCl(2) concentration tested revealed that the addition of CaCl(2) to 1400µM increased the current density by ...	2012	22154401
Shewanella putrefaciens, a rare cause of splenic abscess.	Splenic abscess is uncommon and is still associated with significant morbidity and mortality. Gram-negative bacilli are the most commonly isolated organisms, followed by Gram-positive cocci. However, the predominant organisms found depend on the geographic location. Shewanella putrefaciens is a Gram-negative non-fermentative oxidative bacillus found in the environment. Infection usually manifests with a number of clinical syndromes, most commonly as skin or soft tissue infections, typically in p ...	2011	22154677
Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.	Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...	2012	22168454
dissimilatory reduction of extracellular electron acceptors in anaerobic respiration.	an extension of the respiratory chain to the cell surface is necessary to reduce extracellular electron acceptors like ferric iron or manganese oxides. in the last years more and more compounds were revealed to be reduced at the surface of the outer membrane of gram-negative bacteria and the list does not seem to have an end so far. shewanella as well as geobacter strains are model organisms to discover the biochemistry that enables the dissimilatory reduction of extracellular electron acceptors ...	2011	22179232
The spoilage of air-packaged broiler meat during storage at normal and fluctuating storage temperatures.	Bacterial diversity and the major flora present on air-packaged broiler meat during storage at normal (4°C) and fluctuating storage temperatures (0-4°C and 4-10°C) were investigated using culture-dependent and culture-independent approaches. Culture-dependent analysis revealed that the growth of microflora was retarded when broiler meat was stored at lower temperatures (0-4°C). Denaturing gradient gel electrophoresis profiles showed that Staphylococcus spp., Pseudomonas spp., Acinetobacter spp., ...	2012	22184446
glygly-cterm and rhombosortase: a c-terminal protein processing signal in a many-to-one pairing with a rhomboid family intramembrane serine protease.	the rhomboid family of serine proteases occurs in all domains of life. its members contain at least six hydrophobic membrane-spanning helices, with an active site serine located deep within the hydrophobic interior of the plasma membrane. the model member glpg from escherichia coli is heavily studied through engineered mutant forms, varied model substrates, and multiple x-ray crystal studies, yet its relationship to endogenous substrates is not well understood. here we describe an apparent membr ...	2011	22194940
Effects of disinfectant and biofilm on the corrosion of cast iron pipes in a reclaimed water distribution system.	The effects of disinfection and biofilm on the corrosion of cast iron pipe in a model reclaimed water distribution system were studied using annular reactors (ARs). The corrosion scales formed under different conditions were characterized by X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), and scanning electron microscopy (SEM), while the bacterial characteristics of biofilm on the surface were determined using several molecular methods. The corrosion scales from the ARs with chlor ...	2011	22209261
A biogeochemical framework for bioremediation of plutonium(V) in the subsurface environment.	Accidental release of plutonium (Pu) from storage facilities in the subsurface environment is a concern for the safety of human beings and the environment. Given the complexity of the subsurface environment and multivalent state of Pu, we developed a quantitative biogeochemical framework for bioremediation of Pu(V)O(2) (+) in the subsurface environment. We implemented the framework in the biogeochemical model CCBATCH by expanding its chemical equilibrium for aqueou ...	2012	22209805
removal of methylmercury and tributyltin (tbt) using marine microorganisms.	two marine species of bacteria were isolated that are capable of degrading organometallic contaminants: pseudomonas balearica, which decomposes methylmercury; and shewanella putrefaciens, which decomposes tributyltin. p. balearica decomposed 97% of methylmercury (20.0 μg/l) into inorganic mercury after 3 h, while s. putrefaciens decomposed 88% of tributyltin (55.3 μg sn/l) in real wastewater after 36 h. these data indicate that the two bacteria efficiently decomposed the targeted substances and ...	2012	22212416
shewanella algae bacteremia in a preterm newborn.		2012	22217974
microbial carbon capture cell using cyanobacteria for simultaneous power generation, carbon dioxide sequestration and wastewater treatment.	microbial carbon capture cells (mccs) were constructed with cyanobacteria growing in a photo biocathode in dual-chambered flat plate mediator-less mfcs separated by an anion exchange membrane from the anode compartment containing shewanella putrefaciens. the performance of the mcc with anabaena sparged with co(2)-air mixture was compared with that of a conventional cathode sparged with air only. the power densities achieved were 57.8mw/m(2) for anabaena sparged with a co(2)-air mixture, 39.2mw/m ...	2011	22221988
transcriptome analysis of pellicle formation of shewanella oneidensis.	although the pellicle is one of the major growth modes of microorganisms, the metabolic features of pellicle cells and the determinative factors for pellicle formation are largely unknown. in recent years, biofilm development of shewanella oneidensis, an important model organism for bioremediation studies, has been extensively studied. in this paper, a transcriptional profiling of pellicle cells relative to planktonic cells indicated that cells in pellicles were more metabolically active than th ...	2012	22228442
role of polar and nonpolar residues at the active site for ppiase activity of fkbp22 from shewanella sp. sib1.	fkbp22 from the psychotropic bacterium shewanella sp. sib1 is a homodimeric protein with peptidyl prolyl cis-trans isomerase (ppiase) activity. according to a tertiary model, several nonpolar residues including trp157 and phe197 form a substrate-binding cavity, and asp137 and arg142, which form a salt bridge, are located at the edge of this cavity. to analyze the role of these residues, nine single (d137a, r142a, w157a/f/y, f197a/l/y/w) and one double (d137a/r142a) mutant proteins of sib1 fkbp22 ...	2012	22244380
coastal seawater bacteria harbor a large reservoir of plasmid-mediated quinolone resistance determinants in jiaozhou bay, china.	diversity and prevalence of plasmid-mediated quinolone resistance determinants were investigated in environmental bacteria isolated from surface seawater of jiaozhou bay, china. five qnr gene alleles were identified in 34 isolates by pcr amplification, including qnra3 gene in a shewanella algae isolate, qnrb9 gene in a citrobacter freundii isolate, qnrd gene in 22 proteus vulgaris isolates, qnrs1 gene in 1 enterobacter sp. and 4 klebsiella spp. isolates, and qnrs2 gene in 1 pseudomonas sp. and 4 ...	2012	22252223
involvement of cytochrome c cyma in the anaerobic metabolism of rdx by shewanella oneidensis mr-1.	hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) is a cyclic nitramine explosive commonly used for military applications that is responsible for severe soil and groundwater contamination. in this study, shewanella oneidensis mr-1 was shown to efficiently degrade rdx anaerobically (3.5 µmol·h(-1)·(g protein)(-1)) via two initial routes: (1) sequential n-no(2) reductions to the corresponding nitroso (n-no) derivatives (94% of initial rdx degradation) and (2) denitration followed by ring cleavage. t ...	2012	22260206
adenylylation control by intra- or intermolecular active-site obstruction in fic proteins.	fic proteins that are defined by the ubiquitous fic (filamentation induced by cyclic amp) domain are known to catalyse adenylylation (also called ampylation); that is, the transfer of amp onto a target protein. in mammalian cells, adenylylation of small gtpases through fic proteins injected by pathogenic bacteria can cause collapse of the actin cytoskeleton and cell death. it is unknown how this potentially deleterious adenylylation activity is regulated in the widespread fic proteins that are f ...	2012	22266942