Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| dissemination of blacmy-2 among escherichia coli isolates from food animals, retail ground meats, and humans in southern taiwan. | twenty-six escherichia coli isolates recovered from food animal feces and retail ground meats and 14 urinary e. coli isolates from outpatients were shown to carry bla(cmy-2). similar cmy-2-encoding plasmids were found among seven human and three ground-pork isolates. these data indicate the community spread of bla(cmy-2) in southern taiwan. | 2004 | 15047542 |
| receptor binding domain of escherichia coli f18 fimbrial adhesin fedf can be both efficiently secreted and surface displayed in a functional form in lactococcus lactis. | adherence of f18 fimbrial escherichia coli to porcine intestinal epithelial cells is mediated by the adhesin (fedf) of f18 fimbriae. in a previous study, we demonstrated the specificity of the amino acid residues between 60 and 109 as the receptor binding domain of fedf. in this study, different expression, secretion, and anchoring systems for the receptor binding domain of the fedf adhesin in lactococcus lactis were evaluated. two partially overlapping receptor binding domains (42 and 62 amino ... | 2004 | 15066797 |
| reduction of escherichia coli o157:h7 and salmonella enterica serovar enteritidis in chicken manure by larvae of the black soldier fly. | green fluorescent protein-labeled escherichia coli o157:h7 and salmonella enterica serovar enteritidis were inoculated at 10(7) cfu/g into cow, hog, or chicken manure. ten- or 11-day-old soldier fly larvae (hermetia illucens l.) (7 to 10 g) were added to the manure and held at 23, 27, or 32 degrees c for 3 to 6 days. soldier fly larvae accelerated inactivation of e. coli o157:h7 in chicken manure but had no effect in cow manure and enhanced survival in hog manure. the initial ph values of the ho ... | 2004 | 15083719 |
| endotoxin pretreatment modifies peristalsis and attenuates the antipropulsive action of adrenoceptor agonists in the guinea-pig small intestine. | the action of endotoxin to alter gastrointestinal motility in vivo may reflect a direct effect on the gut or result from vascular and other systemic manifestations of this sepsis model. here we examined whether in vivo pretreatment of guinea-pigs with endotoxin modifies peristalsis in the isolated gut and influences the antipropulsive action of adrenoceptor agonists. distension-induced peristalsis was recorded in fluid-perfused segments of the small intestine taken from animals pretreated intrap ... | 2004 | 15086875 |
| actinobacillus pleuropneumoniae metalloprotease: cloning and in vivo expression. | the complete amino acid and nucleotide sequence of a secreted metalloprotease produced by actinobacillus pleuropneumoniae serotype 1 is reported. a clone showing proteolytic activity in cell-free culture media was selected from a genomic library of a. pleuropneumoniae serotype 1 in puc 19. the sequence obtained contained an open reading frame encoding a protein with 869 amino acids. this protein was identified as a zinc neutral-metalloprotease belonging to the aminopeptidase family, with a predi ... | 2004 | 15109723 |
| genotyping of fimbrial adhesins in escherichia coli strains isolated from slovak piglets suffering from diarrhea. | one-hundred sixty escherichia coli isolates obtained from piglets with diarrhea from different parts of slovakia were examined for the presence of genes coding for f4, f5, f6 and f41 fimbrial adhesins, and hemolytic activity. according to polymerase chain reaction tests 74 (46%) e. coli isolates were positive for primers that detected genes coding for fimbrial adhesins. of these 74 isolates, 64 were positive for genes encoding for f4+, four for f5+, five for f6+, and one for both f41+ and f5+ ad ... | 2004 | 15114867 |
| enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement. | the inclusion of phytase in monogastric animal feed has the benefit of hydrolyzing indigestible plant phytate (myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate) to provide poultry and swine with dietary phosphorus. an ideal phytase supplement should have a high temperature tolerance, allowing it to survive the feed pelleting process, a high specific activity at low phs, and adequate gastric performance. for this study, the performance of a bacterial phytase was optimized by the use of gene ... | 2004 | 15128565 |
| gemifloxacin: a new fluoroquinolone. | gemifloxacin is a dual targeted fluoroquinolone with potent in vitro activity against gram-positive, -negative and atypical human pathogens--pathogens considered to be important causes of community-acquired respiratory tract infections. gemifloxacin demonstrates impressive minimal inhibitory concentrations (mic 90 ) values against clinical isolates of streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, chlamydia pneumoniae and legionella spp., with mic 90 values reported to ... | 2004 | 15155113 |
| molecular cloning of haemoglobin-binding protein hgba in the outer membrane of actinobacillus pleuropneumoniae. | from the porcine pathogen actinobacillus pleuropneumoniae cultivated in iron-deficient or haem-deficient media, haemoglobin (hb)-agarose affinity purification was exploited to isolate an outer-membrane protein of approximately 105 kda, designated hgba. internal peptide sequences of purified hgba were used to design oligonucleotide primers for pcr amplification, yielding amplicons that showed partial sequences with homology to hgba of pasteurella multocida. upon screening two genomic libraries of ... | 2004 | 15184559 |
| relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of enterotoxigenic escherichia coli that produces multiple enterotoxins. | enterotoxigenic escherichia coli (etec) strains that produce multiple enterotoxins are important causes of severe dehydrating diarrhea in human beings and animals, but the relative importance of these enterotoxins in the pathogenesis is poorly understood. gnotobiotic piglets were used to study the importance of heat-labile enterotoxin (lt) in infection with an etec strain that produces multiple enterotoxins. lt(-) (deltaeltab) and complemented mutants of an f4(+) lt(+) stb(+) east1(+) etec strai ... | 2004 | 15213135 |
| nutritional value of a corn containing a glutamate dehydrogenase gene for growing pigs. | eight female pic line 42 pigs (initial bw = 47.5 +/- 1.8 kg) were used in a two-period switchback design (n = 4 per treatment per period) to evaluate the nutritional difference between a genetically modified corn and a similar nontransgenic corn. the genetically altered corn (gdha+) contained a glutamate dehydrogenase gene isolated from escherichia coli. the non-transgenic corn was the same variety lacking the transgenic cassette, grown at the same two locations. pigs were surgically fitted with ... | 2004 | 15216996 |
| prevalence estimation and risk factors for escherichia coli o157 on dutch dairy farms. | to estimate the prevalence of escherichia coli o157 on dutch dairy herds, faecal samples were collected once from 678 randomly selected dairy farms in the period october 1996-december 2000. samples were cultured for e. coli o157. thirty-eight isolates were tested for virulence genes (eae, vt1 and vt2). a questionnaire about farm characteristics was taken from the farm manager, resulting in variables that could be analysed to identify and quantify factors associated with presence of e. coli o157. ... | 2004 | 15219969 |
| aggregation of lactobacilli and bifidobacteria with escherichia coli o157. | a total of 5 bifidobacterium spp. isolated from pig and children' feces and 6 lactobacillus spp. from chicken feces were examined for expression of aggregation, cell surface hydrophobicity (csh) and adherence to intestinal mucin. co-aggregation activity was seen in 3 strains of auto-aggregative bifidobacteria and 4 auto-aggregative strains of lactobacillus spp. with 2 enterohemorrhagic e. coli (o157). csh correlated with lactobacillus auto-aggregating activity and adherence to mucin but the corr ... | 2004 | 15227785 |
| pulmonary net release of tissue-type plasminogen activator during porcine primary and secondary acute lung injury. | tissue-type plasminogen activator (tpa) is a key mediator of fibrinolysis. matching of pulmonary perfusion and ventilation is a critical denominator of oxygenation in acute lung injury (ali). this study investigates pulmonary venoarterial plasma tpa gradients in association with acute ali induced by bronchoalveolar lavage (bal) and endotoxinemia (etx). | 2004 | 15242428 |
| dissemination amongst humans and food products of animal origin of a salmonella typhimurium clone expressing an integron-borne oxa-30 beta-lactamase. | characterization of the molecular basis for beta-lactam resistance and evaluation of the clonal relatedness among nine isolates of multidrug-resistant salmonella typhimurium recovered from seven clinical human samples and two pork end products. | 2004 | 15243023 |
| inhibitory activities of colicins against escherichia coli strains responsible for postweaning diarrhea and edema disease in swine. | the efficacies of colicins e1 and n against escherichia coli strains responsible for postweaning diarrhea and edema disease, two of the most prevalent disease problems for pigs in the united states, were determined in vitro. these proteins may provide an environmentally sound means for the prevention of these infections in swine. | 2004 | 15273129 |
| pharmacokinetics of amoxicillin after oral administration in recently weaned piglets with experimentally induced escherichia coli subtype o149:f4 diarrhea. | to measure the effect of escherichia coli subtype o149:f4-induced diarrhea on the pharmacokinetics of orally administered amoxicillin in affected piglets relative to that of uninfected piglets. | 2004 | 15281660 |
| characterisation of attaching-effacing escherichia coli isolated from animals at slaughter in england and wales. | escherichia coli isolates were recovered from faecal samples taken from cattle, sheep and pigs at slaughter in england and wales. isolates (n = 1227) selected at random from this collection were each hybridised in colony dot-blot experiments with an eae gene probe that presumptively identified attaching-effacing e. coli (aeec). of the 99 (8.1%) eae positive isolates 72 were of ovine origin, 24 were of bovine origin and three of porcine origin. none were typed as o157:h7 whereas 78 were assigned ... | 2004 | 15288926 |
| sample size, library composition, and genotypic diversity among natural populations of escherichia coli from different animals influence accuracy of determining sources of fecal pollution. | a horizontal, fluorophore-enhanced, repetitive extragenic palindromic-pcr (rep-pcr) dna fingerprinting technique (hferp) was developed and evaluated as a means to differentiate human from animal sources of escherichia coli. box a1r primers and pcr were used to generate 2,466 rep-pcr and 1,531 hferp dna fingerprints from e. coli strains isolated from fecal material from known human and 12 animal sources: dogs, cats, horses, deer, geese, ducks, chickens, turkeys, cows, pigs, goats, and sheep. hfer ... | 2004 | 15294775 |
| increased site 1 affinity improves biopotency of porcine growth hormone. evidence against diffusion dependent receptor dimerization. | based on phage display optimization studies with human growth hormone (gh), it is thought that the biopotency of gh cannot be increased. this is proposed to be a result of the affinity of the first receptor for hormone far exceeding that which is required to trap the hormone long enough to allow diffusion of the second receptor to form the ternary complex, which initiates signaling. we report here that despite similar site 1 kinetics to the hgh/hgh receptor interaction, the potency of porcine gh ... | 2004 | 15297460 |
| antibiotic resistance among enterotoxigenic escherichia coli from piglets and calves with diarrhea. | in vitro resistance to 8 antimicrobials among enterotoxigenic escherichia coli from piglets and calves over a 13-year period was evaluated. least resistance occurred against ceftiofur for all, followed by apramycin and gentamicin for porcine and florfenicol for bovine isolates. no significant differences were found between the first 8 and last 5 years. | 2004 | 15317393 |
| mycoplasma hyopneumoniae p65 surface lipoprotein is a lipolytic enzyme with a preference for shorter-chain fatty acids. | mycoplasma hyopneumoniae is the most significant bacterial pathogen of the respiratory tract of swine. p65 is an immunodominant surface lipoprotein of m. hyopneumoniae that is specifically recognized during disease. analysis of the translated amino acid sequence of the gene encoding p65 revealed similarity to the gdsl family of lipolytic enzymes. to examine the lipolytic activity of p65, the gene was cloned and expressed in escherichia coli after truncation of the prokaryotic lipoprotein signal ... | 2004 | 15317784 |
| biological activity, membrane-targeting modification, and crystallization of soluble human decay accelerating factor expressed in e. coli. | decay-accelerating factor (daf, cd55) is a glycophosphatidyl inositol-anchored glycoprotein that regulates the activity of c3 and c5 convertases. in addition to understanding the mechanism of complement inhibition by daf through structural studies, there is also an interest in the possible therapeutic potential of the molecule. in this report we describe the cloning, expression in escherichia coli, isolation and membrane-targeting modification of the four short consensus repeat domains of solubl ... | 2004 | 15322283 |
| crystal structures of unbound and aminooxyacetate-bound escherichia coli gamma-aminobutyrate aminotransferase. | the x-ray crystal structures of escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. the enzyme crystallizes from ammonium sulfate solutions in the p3(2)21 space group with a tetramer in the asymmetric unit. diffraction data were collected to 2.4 a resolution for the unliganded enzyme and 1.9 a resolution for the aminooxyacetate complex. the overall structure of the enzyme is similar to those of other aminotransferase subgroup ii ... | 2004 | 15323550 |
| influence of animal origin and lineage on survival of escherichia coli o157:h7 strains in strong and weak acid challenges. | twenty-five strains of escherichia coli o157:h7 isolated from humans, cattle, and pigs were maintained in hcl (ph 2.5) and in a volatile fatty acid (vfa) mixture (ph 4.0) for up to 6 h at 37 degrees c to assess their ability to survive in acidic conditions that simulate those of the stomach and ileum, respectively. in hcl, the average group survival of bovine strains was significantly higher than that of porcine and human strains, whereas in vfas, porcine strains were significantly more resistan ... | 2004 | 15330520 |
| redox modulation of tau and microtubule-associated protein-2 by the glutathione/glutaredoxin reductase system. | alterations in the redox status of proteins have been implicated in the pathology of several neurodegenerative diseases including alzheimer's and parkinson's. we report that peroxynitrite and h2o2-induced disulfides in the porcine brain microtubule-associated proteins tau and microtubule-associated protein-2 are substrates for the glutaredoxin reductase system composed of glutathione reductase, human or escherichia coli glutaredoxin, reduced glutathione, and nadph. oxidation and reduction of cys ... | 2004 | 15351709 |
| effect of antimicrobial agents on the production and release of shiga toxin by enterotoxaemic escherichia coli isolates from pigs. | edema disease (ed) of pigs is an enterotoxaemic disease caused by enterotoxaemic escherichia coli (eteec) infection. antimicrobial therapy for pigs with ed is controversial because it may induce death of sickish piglets. in this study, we investigated the effects in vitro of 7 antimicrobial agents, ampicillin, gentamicin, colistin, bicozamycin, fosfomycin, sulfamethoxazole-trimethoprim and enrofloxacin, on the release and production of shiga toxin (stx) 2e by eteec strains. we found that more st ... | 2004 | 15353838 |
| molecular characterization of extraintestinal pathogenic escherichia coli (expec) pathogenicity islands in f165-positive e. coli strain from a diseased animal. | septicemic escherichia coli 4787 (o115: k-: h51: f165) of porcine origin possess gene clusters related to extraintestinal e. coli fimbrial adhesins. this strain produces two fimbriae: f165(1) and f165(2). f165(1) (prs-like) belongs to the p fimbrial family, encoded by foo operon and f165(2) is a f1c-like encoded by fot operon. data from this study suggest that these two operons are part of two pais. pai i(4787) includes a region of 20 kb, which not only harbors the foo operon but also contains a ... | 2004 | 15358417 |
| fimbrial subunit protein faeg expressed in transgenic tobacco inhibits the binding of f4ac enterotoxigenic escherichia coli to porcine enterocytes. | plants offer a promising alternative for the production of foreign proteins for pharmaceutical purposes in tissues that are consumed as food and/or feed. our long-term strategy is to develop edible vaccines against piglet diarrhoea caused by enterotoxigenic escherichia coli (f4 etec) in feed plants. in this work, we isolated a gene, faeg, encoding for a major f4ac fimbrial subunit protein. our goal was to test whether the faeg protein, when isolated from its fimbrial background and produced in a ... | 2004 | 15359606 |
| specific interaction between the classical swine fever virus ns5b protein and the viral genome. | the ns5b protein of the classical swine fever virus (csfv) is the rna-dependent rna polymerase of the virus and is able to catalyze the viral genome replication. the 3' untranslated region is most likely involved in regulation of the pestivirus genome replication. however, little is known about the interaction between the csfv ns5b protein and the viral genome. we used different rna templates derived from the plus-strand viral genome, or the minus-strand viral genome and the csfv ns5b protein ob ... | 2004 | 15373834 |
| a european survey of antimicrobial susceptibility among zoonotic and commensal bacteria isolated from food-producing animals. | to study antimicrobial resistance in zoonotic bacteria isolated from food animals in different countries using uniform methodology. | 2004 | 15375107 |
| prevalence of serogroups and virulence factors of escherichia coli strains isolated from pigs with postweaning diarrhoea in eastern china. | this study was undertaken to determine the present distribution of serogroups, hemolytic activity and virulence factors among escherichia coli strains isolated from pigs with postweaning diarrhoea from eight provinces in eastern china. two hundred and fifteen e. coli isolates were serogrouped with o-antisera, investigated for hemolytic activity, assessed for f4, f5, f6, f18 and f41 fimbrial antigens by monoclonal antibodies and detected for genes of enterotoxins and shiga-toxin-two-variant (stx2 ... | 2004 | 15381261 |
| long polar fimbriae contribute to colonization by escherichia coli o157:h7 in vivo. | the contribution of long polar fimbriae to intestinal colonization by escherichia coli o157:h7 was evaluated in sheep, conventional pigs, and gnotobiotic piglets. e. coli o157:h7 strains with lpfa1 and lpfa2 mutated were recovered in significantly lower numbers and caused fewer attachment and effacement lesions than the parent strain. | 2004 | 15385526 |
| gene cloning and expression of an alkaline serine protease with dehairing function from bacillus pumilus. | a new gene (named ap gene) encoding an alkaline serine protease with dehairing function was cloned from bacillus pumilus un-31-c-42 and its nucleotide sequence was determined. the expression of ap gene was induced with iptg in escherichia coli after the mature protease region was cloned into pet15b and sds-page showed expressed product clearly, but no alkaline protease activity was detected. in order to express the ap gene in b. subtilis, a recombinant expression plasmid was constructed which co ... | 2004 | 15386098 |
| addition of pearl barley to a rice-based diet for newly weaned piglets increases the viscosity of the intestinal contents, reduces starch digestibility and exacerbates post-weaning colibacillosis. | the purposes of the present study were to investigate the effects of feeding a cereal grain containing nsp on body growth and the intestinal microenvironment of recently weaned pigs, and to examine resultant associations with pathogenic escherichia coli in the intestinal tract. in expt 1, pearl barley, a grain rich in soluble nsp, was incorporated (250, 500 or 750 g/kg diet) into a low-fibre control diet based on cooked white rice and fed for 7-10 d following weaning. consumption of pearl barley ... | 2004 | 15469645 |
| field evaluation of the effect of a probiotic-containing bacillus licheniformis and bacillus subtilis spores on the health status, performance, and carcass quality of grower and finisher pigs. | the aim of the study was to assess the efficacy of bioplus 2b, a probiotic containing bacillus licheniformis and b. subtilis spores, on the health status and productivity of pigs, during weaning, growing and finishing stages of growth. on a commercial farrow-to-finish farm, five experimental groups were formed, each of 54 weaned piglets. the pigs of the first group (double controls) received normal feed with no probiotic and the pigs of the second group (untreated controls) received bioplus 2b o ... | 2004 | 15485567 |
| [cloning and expression of pituitary prolactin gene in ailuropoda melanoleuca]. | the giant panda (ailuropoda melanoleuca) is an endangered species and indigenous to china. it has been proposed that it has a highly specialized reproductive pattern with low fecundity, but little is known about its basic reproductive biology at molecular level. in this study,the pituitary prolactin (prl) cdna of giant panda was amplified by rt-pcr from pituitary total rna and then cloned, sequenced and submitted to genbank (genbank accession no. ay161285). the sequence analysis revealed that th ... | 2004 | 15493147 |
| adverse biochemical and physiological effects of prostacyclin in experimental brain oedema. | prostacyclin (pgi2) and its stable analogues are known to reduce capillary hydraulic permeability. this study explores the biochemical and physiological effects of i.v. infusion of low-dose pgi2 in an experimental model of vasogenic brain oedema. | 2004 | 15504195 |
| tumor necrosis factor-alpha, interleukin-6, serum amyloid a, haptoglobin, and cortisol concentrations in sows following intramammary inoculation of escherichia coli. | to determine whether concentrations of proinflammatory cytokines, acute-phase proteins, and cortisol differ at parturition among 3 categories of sows (noninoculated, clinically affected and nonaffected following intramammary inoculation with escherichia coll). | 2004 | 15524332 |
| virulence characteristics of escherichia coli isolates obtained from broiler breeders with salpingitis. | thirty isolates of escherichia coli from broiler breeders with salpingitis were studied. using the slide agglutination test, the isolates were found to belong to serogroups o1, o2, o5, o36, o45, o53 and o78. pathogenicity for day-old chicks was determined by air sac inoculation and isolates were categorized as having high, intermediate or low virulence. growth on iron starvation medium was observed together with aerobactin production. based on the results of in vitro adherence tests, attachment ... | 2005 | 15563950 |
| possible structure and function of the extra c-terminal sequence of pyruvate kinase from bacillus stearothermophilus. | the pyruvate kinases from genus bacillus and a few other bacteria have an extra c-terminal sequence with a phosphoenolpyruvate binding motif composed of about 110 amino acids. to elucidate the possible structure and function of this sequence, the enzyme lacking the sequence was prepared and characterized. the n-terminal sequences of the peptides, which were found only in the lysylendopeptidase digest of the wild enzyme and not in that of the truncated enzyme, were determined. all the determined ... | 2004 | 15625316 |
| cholera toxin improves the f4(k88)-specific immune response following oral immunization of pigs with recombinant faeg. | oral immunization of both humans and animals with non-replicating soluble antigens often results in the induction of oral tolerance. however, receptor-dependent uptake of orally administered soluble antigens can lead to the induction of an antigen-specific immune response. indeed, oral immunization of pigs with recombinant faeg (rfaeg), the adhesin of the f4(k88) fimbriae of enterotoxigenic escherichia coli (etec), induces an f4-specific humoral and cellular immune response. this response is acc ... | 2005 | 15626459 |
| antibacterial effects of the cu(ii)-exchanged montmorillonite on escherichia coli k88 and salmonella choleraesuis. | the aim of this research was to determine the antibacterial properties and mechanisms of cu(ii)-exchanged montmorillonite (mmt-cu) in vitro. escherichia coli atcc k88 and salmonella choleraesuis atcc 50020 were chosen as indicators of intestinal tract pathogenic bacteria in weanling pigs. the antibacterial activity of mmt-cu and mmt were evaluated by determining the minimum inhibitory concentrations (mics) using two-fold serial dilutions in mh broth, and the amount of cu2+ released into the brot ... | 2004 | 15627522 |
| [hazard evaluation of livestock-derived, verotoxin-producing escherichia coli by enterohemolysin production assay and eaea gene detection]. | we examined enterohemolysin (ehly) production, and detected the hlya gene and the eaea gene for the intestinal mucosal adherence factor intimin in 131 strains of human-derived verotoxin-producing escherichia coli (vtec) and 140 strains of livestock (cattle and swine) -derived vtec to evaluate their hazards to humans. the hlya gene was confirmed in 98.5% of human-derived, in 50.5% of cattle-derived, and in 10.3% of swine-derived vtec strains. ehly-positive rates were 96.2-97.7%, 45.9-55.0%, and 1 ... | 2004 | 15628530 |
| attaching-effacing bacteria in animals. | enteric bacteria with a demonstrable or potential ability to form attaching-effacing lesions, so-called attaching-effacing (ae) bacteria, have been found in the intestinal tracts of a wide variety of warm-blooded animal species, including man. in some host species, for example cattle, pigs, rabbits and human beings, attaching-effacing escherichia coli (aeec) have an established role as enteropathogens. in other host species, ae bacteria are of less certain significance. with continuing advances ... | 2005 | 15629476 |
| conformation and activity of delta-lysin and its analogs. | delta-lysin is a 26-residue hemolytic peptide secreted by staphylococcus aureus. unlike the bee venom peptide melittin, delta-lysin does not exhibit antibacterial activity. we have synthesized delta-lysin and several analogs wherein the n-terminal residues of the toxin were sequentially deleted. the toxin has three aspartic acids, four lysines and no prolines. analogs were also generated in which all the aspartic acids were replaced with lysines. a proline residue was introduced in the native se ... | 2005 | 15629533 |
| occurrence and behavior of enterobacteriaceae and enterococci in mediterranean dry sausages during ripening in a pilot-scale chamber. | the occurrence and evolution of enterobacteriaceae, coliforms, escherichia coli, and enterococci in 252 samples of six types of mediterranean dry-ripened sausages during maturation was investigated. the changes of the ph values were also recorded. samples were analyzed in three steps of the ripening process: fresh product, first drying stage, and finished product. in all six sausages, enterobacteriaceae, coliforms, and e. coli counts were characterized by high initial concentrations that signifi ... | 2004 | 15633693 |
| antibody responses of pigs to defined erns fragments after infection with classical swine fever virus. | antibody responses of pigs to defined erns fragments, after classical swine fever virus (csfv) infection, were studied by using an enzyme-linked immunosorbent assay (elisa). selection of various e(rns) fragments was based on an immunodominant erns region encompassing three overlapping antigenic regions, amino acids 65 to 145 (erns(aa)65-145) (ar1), 84 to 160 (erns(aa)84-160) (ar2), and 109 to 220 (erns(aa)109-220) (ar3), identified earlier by our group (m. lin, e. trottier, j. pasick, and m. sab ... | 2005 | 15643004 |
| antimicrobial activity of five essential oils against origin strains of the enterobacteriaceae family. | an in vitro assay measuring the antimicrobial activity of essential oils of coridothymus capitatus (spanish origanum), satureja montana, thymus mastichina (spanish origanum majorana), thymus zygis (spanish variety of thymus vulgaris) and origanum vulgare has been carried out against poultry origin strains of escherichia coli, salmonella enteritidis and salmonella essen, and pig origin strains of enterotoxigenic e. coli (etec), salmonella choleraesuis and salmonella typhimurium. using the broth m ... | 2005 | 15676008 |
| microencapsulation for the gastric passage and controlled intestinal release of immunoglobulin y. | avian immunoglobulin (ig) y is a promising alternative for the treatment and prevention of enteric infections, and has shown to be effective against a number of gastrointestinal pathogens, including escherichia coli, salmonella, and rotavirus. however, its application is limited by its sensitivity to human gastrointestinal conditions. here, we report on the enteric coating of igy-containing granules with a ph-sensitive methacrylic acid copolymer. a 35% (w/w) polymer coating was found to protect ... | 2004 | 15680164 |
| effect of zinc pretreatment on pulmonary endothelial cells in vitro and pulmonary function in a porcine model of endotoxemia. | during endotoxemia, the systemic inflammatory response often leads to severe pulmonary damages. destruction of endothelial cells, interstitial edema, and interstitial alveolitis depress pulmonary circulation and raise extravascular lung water and intrapulmonary shunt. as protective effects of zinc are described in vitro as well as in vivo, this study investigates its impact on septic porcine pulmonary endothelial monolayers as well as on the pulmonary function of endotoxemic pigs. | 2005 | 15680386 |
| structural model of human gad65: prediction and interpretation of biochemical and immunogenic features. | the 65 kda human isoform of glutamate decarboxylase, gad65, plays a central role in neurotransmission in higher vertebrates and is a typical autoantigen in several human autoimmune diseases, such as insulin-dependent diabetes mellitus (iddm), stiff-man syndrome and autoimmune polyendocrine syndrome type i. in autoimmune diabetes, an attack of inflammatory cells to endocrine pancreatic beta-cells leads to their complete destruction, eventually resulting in the inability to produce sufficient insu ... | 2005 | 15690345 |
| effect of glutamine and spray-dried plasma on growth performance, small intestinal morphology, and immune responses of escherichia coli k88+-challenged weaned pigs. | forty weaned barrows (5.32 +/- 0.3 kg bw) at 17 +/- 2 d of age were used to investigate the effects of feeding glutamine and spray-dried plasma on the growth performance, small intestinal morphology, and immune responses of escherichia coli k88-challenged pigs. pigs were allotted to four treatments including: 1) nonchallenged control (nonc); 2) challenged control (chac); 3) 7% (as-fed basis) spray-dried plasma (sdp); and 4) 2% (as-fed basis) glutamine (gln). on d 11 after weaning, all pigs were ... | 2005 | 15705760 |
| serological diagnosis of enzootic pneumonia of swine by a double-sandwich enzyme-linked immunosorbent assay using a monoclonal antibody and recombinant antigen (p46) of mycoplasma hyopneumoniae. | to facilitate the control of enzootic pneumonia (ep) of swine caused by mycoplasma hyopneumoniae, the complement fixation (cf) test has been used for the detection of m. hyopneumoniae antibodies. however, the cf test is a cumbersome and time-consuming technique and cross-reactivity are major drawbacks associated with this method. to circumvent these drawbacks, we have developed a double-sandwich enzyme-linked immunosorbent assay (elisa), consisting of purified monoclonal antibody (mab) against t ... | 2004 | 15708823 |
| escherichia coli o157:h7 does not require intimin to persist in pigs. | 2005 | 15731090 | |
| investigation of domestic animals and pets as a reservoir for intimin- (eae) gene positive escherichia coli types. | domestic animals belonging to seven different species (cattle, sheep, dogs, cats, pigs, chicken and goats) were investigated as natural reservoirs for attaching and effacing escherichia coli (aeec). for this, 2165 e. coli strains from faeces of 803 animals were examined for the presence of the intimin -(eae) gene as a characteristic of aeec strains. ten percent of the animals were found to excrete aeec, most frequently found in sheep (19.2%) and pigs (17.6), followed by cattle (10.4%), dogs (7.2 ... | 2005 | 15737477 |
| analyses of livestock production, waste storage, and pathogen levels and prevalences in farm manures. | survey results describing the levels and prevalences of zoonotic agents in 1,549 livestock waste samples were analyzed for significance with livestock husbandry and farm waste management practices. statistical analyses of survey data showed that livestock groups containing calves of <3 months of age, piglets, or lambs had higher prevalences and levels of campylobacter spp. and escherichia coli o157 in their wastes. younger calves that were still receiving milk, however, had significantly lower l ... | 2005 | 15746323 |
| antimicrobial-resistant and extraintestinal pathogenic escherichia coli in retail foods. | extraintestinal escherichia coli infections are associated with specialized extraintestinal pathogenic e. coli (expec) strains and, increasingly, with antimicrobial resistance. the food supply may disseminate expec and antimicrobial-resistant e. coli. | 2005 | 15747237 |
| identification of the outer-membrane protein pagc required for the serum resistance phenotype in salmonella enterica serovar choleraesuis. | serum resistance is a crucial virulence factor for the development of systemic infections, including bacteraemia, by many pathogenic bacteria. salmonella enterica serovar choleraesuis is an important enteric pathogen that causes serious systemic infections in swine and humans. here, it was found that, when introduced into escherichia coli, a recombinant plasmid carrying the pagc gene from a plasmid-based genomic library of s. enterica serovar choleraesuis conferred a high-level resistance to the ... | 2005 | 15758232 |
| different effects of surfactant and inhaled nitric oxide in modulation of inflammatory injury in ventilated piglet lungs. | septic acute lung injury (ali) causes high morbidity and mortality in intensive care service as a result of biotrauma and dysfunction in the lungs and other organ systems. we hypothesized that surfactant and/or inhaled nitric oxide (ino) may have different effects in modulation of inflammatory injury in septic ali. twenty-four healthy, 6-9 kg piglets were anesthetized, and intraperitoneally injected with escherichia coli, followed by a low tidal volume ventilation until sepsis and ali developed ... | 2005 | 15777614 |
| effects of ifn-alpha on the inflammatory response of swine leukocytes to bacterial endotoxin. | because low-dose interferon-alpha (ifn-alpha) treatment had proved effective in several models of chronic inflammation and autoimmune disease, a possible role of ifn-alpha in modulating the response of swine leukocytes to bacterial endotoxin was investigated in this study. exposure of swine peripheral blood mononuclear cells (pbmc) to low concentrations of human ifn-alpha caused a strong, dose-dependent decrease in cd14 expression, the lowest level being observed at 5 u/ml ifn-alpha. this result ... | 2005 | 15812246 |
| prevalence of verotoxin-producing escherichia coli (vtec) 0157 in swedish dairy herds. | a prevalence study of verotoxin-producing escherichia coli o157 (vtec o157) was performed in 371 randomly selected dairy herds distributed throughout sweden. faecal and manure samples were collected and analysed by immunomagnetic separation and culturing. data were recorded for each herd regarding herd size, age of sampled animals and whether, in addition to cattle, the farm kept other animals. vtec o157 was isolated from 33 (8.9%) of the 371 investigated herds. the prevalence was higher (23.3%) ... | 2005 | 15816162 |
| the prevalence of shiga toxin-producing escherichia coli in domestic animals and food in serbia. | faecal samples of 2660 domestic animals from 116 farms and 956 samples of food were examined for the presence of shiga toxin-producing escherichia coli (stec). stec was recovered from 126 (15.3%) cattle, 135 (11.3%) pigs, 135 (66.8%) sheep, 31 (73.8%) goats, 4 (1%) chicken, and 15 (1.6%) food samples. of all stec isolates, 21.5, 25.8 and 15% produced enterohaemolysin, alpha-haemolysin, and aerobactin respectively, 1.6% displayed localized adherence (la) to hep-2 cells, 27.6% were sorbitol negati ... | 2005 | 15816163 |
| biochemical intestinal parameters in germ-free minipigs and rats and in ex-germ-free minipigs and rats monoassociated with escherichia coli. | intestinal contents of newborn and young germ-free minipigs and germ-free rats were investigated for the following biochemical parameters - conversion of cholesterol to coprostanol, degradation of beta-aspartylglycine, level of tryptic activity, formation of urobilinogen and the profile of short-chain fatty acids. additionally, germ-free minipigs and germ-free rats were monoassociated with non-pathogenic strains of escherichia coli and were investigated for the same biochemical parameters. the c ... | 2005 | 15836440 |
| investigation and application of methods for enumerating heterotrophs and escherichia coli in the air within piggery sheds. | to investigate methods for the recovery of airborne bacteria within pig sheds and to then use the appropriate methods to determine the levels of heterotrophs and escherichia coli in the air within sheds. | 2005 | 15836483 |
| oxygen extraction in pigs subjected to low-dose infusion of endotoxin after major abdominal surgery. | sepsis may impair o(2) extraction due to blood flow redistribution or decreased utilization of the available oxygen. | 2005 | 15836675 |
| [high level secretion expression of poifnalpha in pichia pastoris]. | the porcine alpha interferon gene was inserted into the pichia pastoris expression vector of ppiczalphaa which contains aox i promoter and alpha-factor signal sequence. the recombinant plasmid was transformed into host cell e.coli jm109 and then was extracted for analysis of restriction enzymes. it was confirmed that heterogeneous gene spliced into vector ppiczalphaa was ifnalpha gene. the recombinant plasmid of ppiczalphaa-ifnalpha was linearnized by sac i and transformed into km71 by electropo ... | 2005 | 15843348 |
| novel preharvest strategies involving the use of experimental chlorate preparations and nitro-based compounds to prevent colonization of food-producing animals by foodborne pathogens. | foodborne diseases caused by enterohemorrhagic escherichia coli, salmonella, and campylobacter species are of public health and economic significance. shedding of these pathogens during production and slaughter are risks for contamination of products for human consumption. consequently, strategies are sought to prevent or reduce the carriage of these pathogens in food animals before slaughter. experimental products containing chlorate salts have been proven efficacious in reducing concentrations ... | 2005 | 15844824 |
| experimental enteric colibacillosis in piglets. | eleven of 20 piglets inoculated orally with escherichia coli:0101:ku460(a):nm and one of 18 piglets inoculated orally with e. coli:08:k87, k88a, b:h19 developed enteric colibacillosis. successful reproduction of the disease was associated with recovery of a dense growth in a nearly pure culture of the inoculated strain from the feces. piglets which remained clinically normal had none, or very few, of the inoculated strain in their feces. seventeen control piglets inoculated orally with the non-e ... | 1968 | 15846900 |
| expression of porcine lactoferrin by using recombinant baculovirus in silkworm, bombyx mori l., and its purification and characterization. | lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. in this study, we exploited the silkworm, bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (plf) gene to produce the recombinant plf (rplf). around 205 mug of rplf was purified from a single silkworm pupa infected by the virus and the rplf was proved to be biologically active. this method established in our study will pave the way for efficient industrial production ... | 2005 | 15856222 |
| two-cistronic expression plasmids for high-level gene expression in escherichia coli preventing translational initiation inhibition caused by the intramolecular local secondary structure of mrna. | two-cistronic expression plasmids for the wild-type solubilized domain of porcine nadph-cytochrome p450 reductase (pscpr) gene in escherichia coli were systematically constructed using a solubilized domain of porcine cytochrome b5 gene (psb5 gene) or a derivative of it as the first cistron to examine their utility for second gene expression preventing the translational inhibition caused by the intramolecular local secondary structure of mrna at the ribosome-binding site (rbs). the mrnas from the ... | 2005 | 15858177 |
| oral immunization of sows: anti-k88 antibodies in serum and milk of the sow and in serum of the piglets. | pregnant sows were immunized by oral application of live e. coli. the effect of immunization was demonstrated by measuring the titers of anti-k88 antibodies in sow serum, colostrum and milk as well as in piglet sera using enzyme-linked immunosorbent assays. the isotype involved in anti-k88 reactivity was found to be iga. by comparing iga-titers in colostrum and milk, the local production of this ig-class in the mammary gland is suggested. | 1982 | 15861583 |
| characterization of the type i secretion system of the rtx toxin apxii in "actinobacillus porcitonsillarum". | strains of actinobacillus porcitonsillarum are regularly isolated from the tonsils of healthy pigs. a. porcitonsillarum is non pathogenic but phenotypically it strongly resembles the pathogenic species actinobacillus pleuropneumoniae, thereby interfering with the diagnosis of the latter. a. porcitonsillarum is hemolytic but unlike a. pleuropneumoniae, it contains only apxii genes and not apxi or apxiii genes. in contrast to the truncated apxii operon of a. pleuropneumoniae, which lacks the type ... | 2005 | 15863281 |
| characterization of shiga toxin-producing escherichia coli isolated from aquatic environments. | this study reports the phenotypic and genotypic characterization of 144 shiga toxin-producing escherichia coli (stec) strains isolated from urban sewage and animal wastewaters using a shiga toxin 2 gene variant (stx(2))-specific dna colony hybridization method. all the strains were classified as e. coli and belonged to 34 different serotypes, some of which had not been previously reported to carry the stx(2) genes (o8:h31, o89:h19, o166:h21 and o181:h20). five stx(2) subtypes (stx(2), stx(2c), s ... | 2005 | 15869962 |
| construction of a novel shigella live-vector strain co-expressing cs3 and ltb/stm of enterotoxigenic e.coli. | to construct and evaluate a polyvalent recombinant vaccine strain shigella flexneri 2a t32 against enterotoxigenic e.coli (etec). | 2005 | 15948247 |
| escherichia coli o157 prevalence in dutch poultry, pig finishing and veal herds and risk factors in dutch veal herds. | in the period october 1996 through december 2000, a total of 7163 pooled faecal samples of laying hen and broiler flocks, finishing-pig herds and veal herds were examined for the presence of salmonella spp., campylobacter spp. and verocytotoxin-producing escherichia coli o157 as part of a national monitoring programme in the netherlands. isolates were tested for eae and vt genes. risk factors for dutch veal herds were quantified. for all herd/flock types, faecal samples were cultured for e. coli ... | 2005 | 15951037 |
| declines of zoonotic agents in liquid livestock wastes stored in batches on-farm. | to measure the decline rates of zoonotic agents introduced into liquid livestock wastes in on-farm storage tanks. | 2005 | 15960665 |
| using national microbiological data to set meaningful performance criteria for slaughter and dressing of animals at australian export abattoirs. | slaughter establishments in australia that export meat to the usa are required by the controlling authority, the australian quarantine and inspection service (aqis), to test carcases under the escherichia coli and salmonella monitoring (esam) program and to use statistical process control techniques to ensure meat is produced hygienically. however, analysing the esam database for e. coli using standard statistical techniques proved difficult because of inter-plant variability and because the vas ... | 2005 | 15964649 |
| in vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus type ii without nuclear localization signal. | we expressed firstly the capsid protein gene defecting the nuclear localization signal (nls) of porcine circovirus type ii (pcv2) in escherichia coli as a fusion protein with glutathione s-transferase (rgst-dcap protein). the purified rgst-dcap protein and the recombinant nls-defected cap protein of pcv2 (rdcap protein) from the purified rgst-dcap protein reacted specifically with swine antiserum to pcv2. furthermore, the obtained monoclonal antibodies (mabs) to rdcap protein were shown to bind ... | 2005 | 15964652 |
| flexibility of prolyl oligopeptidase: molecular dynamics and molecular framework analysis of the potential substrate pathways. | the flexibility of prolyl oligopeptidase has been investigated using molecular dynamics (md) and molecular framework approaches to delineate the route of the substrate to the active site. the selectivity of the enzyme is mediated by a seven-bladed beta-propeller that in the crystal structure does not indicate the possible passage for the substrate to the catalytic center. its open topology however, could allow the blades to move apart and let the substrate into the large central cavity. flexibil ... | 2005 | 15971204 |
| inhibition of bacterial adherence on the surface of stents and bacterial growth in bile by bismuth dimercaprol. | bacterial infection and biofilm formation on the surface of biliary stents is believed to be one of the main factors in stent occlusion. this study explored the role of the new reagent, bismuth dimercaprol, in preventing bacterial adherence and bacterial biofilm formation on the surface of biliary stents. sterile porcine bile preparations, infected separately with escherichia coli, klebsiella pneumoniae, enterobacter, and enterococcus, were used as the perfusion media in an in vitro perfusion sy ... | 2005 | 15986852 |
| a novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo. | prebiotics are nondigestible food ingredients that encourage proliferation of selected groups of the colonic microflora, thereby altering the composition toward a more beneficial community. in the present study, the prebiotic potential of a novel galactooligosaccharide (gos) mixture, produced by the activity of galactosyltransferases from bifidobacterium bifidum 41171 on lactose, was assessed in vitro and in a parallel continuous randomized pig trial. in situ fluorescent hybridization with 16s r ... | 2005 | 15987856 |
| de novo rna synthesis and homology modeling of the classical swine fever virus rna polymerase. | classical swine fever virus (csfv) non-structural protein 5b (ns5b) encodes an rna-dependent rna polymerase (rdrp), a key enzyme which initiates rna replication by a de novo mechanism without a primer and is a potential target for anti-virus therapy. we expressed the ns5b protein in escherichia coli. the rgtp can stimulate de novo initiation of rna synthesis and mutation of the gdd motif to gly-asp-asp (gaa) abolishes the rna synthesis. to better understand the mechanism of viral rna synthesis i ... | 2005 | 16022897 |
| decline of zoonotic agents in livestock waste and bedding heaps. | to measure the rates of decline of zoonotic agents introduced into heaps of spent bedding and faecal wastes generated by commercially farmed livestock and managed in a similar way to that of a working farm. | 2005 | 16033467 |
| impact of dried skim milk in production diets on lactobacillus and pathogenic bacterial shedding in growing-finishing swine. | to determine the possible effects of inclusion of dried skim milk (dsm) in swine diets on indigenous lactobacillus spp. and escherichia coli, and its potential for controlling pathogen shedding and affect animal growth in growing-finishing swine. | 2005 | 16033472 |
| assessment of the fitness impacts on escherichia coli of acquisition of antibiotic resistance genes encoded by different types of genetic element. | little is known of the fitness cost that antibiotic resistance exerts on wild-type bacteria, especially in their natural environments. we therefore examined the fitness costs that several antibiotic resistance elements imposed on a wild-type escherichia coli isolate, both in the laboratory and in a pig gut colonization model. | 2005 | 16040624 |
| characterization of two major groups of diarrheagenic escherichia coli o26 strains which are globally spread in human patients and domestic animals of different species. | twenty-three escherichia coli o26 strains from humans, cattle, sheep, pigs and chicken were investigated for virulence markers and for genetic similarity by pulsed field gel electrophoresis and multi locus sequence typing. two groups of genetically closely related o26 strains were defined. one group is formed by enteropathogenic (epec) and enterohemorrhagic (ehec) e. coli strains, which do not ferment rhamnose and dulcitol and most of these carry a plasmid encoding enterohemolysin. the other gro ... | 2005 | 16046083 |
| microbial quality of runoff following land application of cattle manure and swine slurry. | concentrations of human health-related microorganisms in runoff from agricultural plots (0.75 m x 2 m) treated with fresh and aged cattle manure, swine slurry and no manure (control) were determined. three consecutive simulated rainfall events, producing 35 mm rainfall and separated by 24 h, were carried out for each plot. fecal indicator (escherichia coli, enterococci, clostridium perfringens and coliphage) loads released in rainfall runoff from plots treated with fresh cattle manure, aged catt ... | 2005 | 16075941 |
| effects of nitric oxide in 5-hydroxytryptamine-, cholera toxin-, enterotoxigenic escherichia coli- and salmonella typhimurium-induced secretion in the porcine small intestine. | the effects of nitric oxide (no) in the secretory response to the endogenous secretagogue 5-hydroxytryptamine (5-ht), the enterotoxins heat-labile enterotoxigenic escherichia coli (etec) toxin (lt) and cholera toxin (ct), and various cultures of etec and salmonella serotype typhimurium in the porcine small intestine (sus scrofa) were investigated. in anaesthetized pigs, jejunal tied-off loops were instilled with 5-ht, lt, ct, various cultures of etec or s. typhimurium. pigs were given intravenou ... | 2005 | 16098780 |
| host immune status influences the development of attaching and effacing lesions in weaned pigs. | attaching and effacing escherichia coli (aeec) has been associated with naturally occurring attaching and effacing (a/e) lesions in weaned pigs, and although a/e lesions have been experimentally reproduced in newborn piglets, such lesions have been much more difficult to induce in older conventional pigs. hence, the aim of this study was to examine the effect of oral administration of dexamethasone on the development of a/e lesions in weaned pigs challenged with a porcine enteropathogenic e. col ... | 2005 | 16113267 |
| interaction of enteropathogenic and shiga toxin-producing escherichia coli and porcine intestinal mucosa: role of intimin and tir in adherence. | the ileal in vitro organ culture (ivoc) model using tissues originating from colostrum-deprived newborn piglets has proven to be an effective way to study the attaching and effacing (a/e) phenotype of porcine enteropathogenic escherichia coli (epec) ex vivo. the aim of this study was to investigate the role of intimin subtype and tir in the adherence of epec and shiga-toxin-producing e. coli (stec), isolated from different animal species, to porcine intestinal ivoc. moreover, the role of intimin ... | 2005 | 16113321 |
| identification of putative adhesin genes in shigatoxigenic escherichia coli isolated from different sources. | shiga toxin-producing escherichia coli (stec) is an important pathogen responsible for severe human intestinal and systemic infections. the bacterial factors required for colonization of the hosts are still not well defined. in this study, the prevalence of seven putative adhesive genes that are not encoded in the locus of enterocyte effacement (lee) in 74 stec strains isolated from humans (n=39), food (n=6), cattle (n=11), and pigs (n=18) was investigated by pcr. in addition, shiga toxin (stx) ... | 2005 | 16125342 |
| comparison of faecal and optimal growth conditions on in vitro pharmacodynamic activity of marbofloxacin against escherichia coli. | the objective of the study was to compare the in vitro activity of marbofloxacin against escherichia coli (e. coli) strains with differing marbofloxacin susceptibility levels under optimal growth conditions and under condition mimicking faecal environment in time-kill kinetic studies. under optimal growth conditions, marbofloxacin exerted a bactericidal concentration-dependent activity against all e. coli strains with bactericidal concentrations equal to 1 or 4 times mic. under faecal growth con ... | 2006 | 16126240 |
| structural aspects and biological properties of the cathelicidin pmap-36. | pmap-36 is a cathelicidin-derived host defence peptide originally deduced by a transcript from pig bone marrow rna. the expression of the propeptide in leukocytes, and the structure, antimicrobial activity, and mechanism of action of the mature peptide were investigated. the proform is stored as a dimeric precursor of 38 kda formed by a dimerization site at its c-terminal cysteine residue; it is likely that the mature peptide is dimeric when released. monomeric and dimeric forms of pmap-36 were ... | 2005 | 16128809 |
| effects of perfluorohexan vapor on gas exchange, respiratory mechanics, and lung histology in pigs with lung injury after endotoxin infusion. | inhaled perfluorohexan vapor has been shown to improve gas exchange and pulmonary mechanics in oleic acid- and ventilator-induced lung injury. however, in the clinical setting, lung injury frequently occurs in the context of systemic inflammation and consecutive lung injury, which may be induced experimentally by intravenous administration of endotoxin. the authors studied whether vaporized perfluorohexan is efficacious during endotoxin-induced lung injury in domestic pigs. | 2005 | 16129984 |
| inheritance of the f4ab, f4ac and f4ad e. coli receptors in swine and examination of four candidate genes for f4acr. | susceptibility to enterotoxigenic escherichia coli with fimbriae f4ac is dominantly inherited in the pig. a three-generation pedigree was created to refine the position of f4acr on chromosome 13 comprising 202 pigs: eight parents, 18 f1 and 176 f2 pigs. the 17-point analysis indicates that f4acr lies between sw207 and s0283. recombinant offspring specify that the most probable order is sw207-s0075-f4acr-sw225-s0283. we observed six phenotypes for the three fimbrial variants f4ab, f4ac and f4ad. ... | 2005 | 16130451 |
| changes in patterns of antimicrobial susceptibility and class 1 integron carriage among escherichia coli isolates. | the worldwide use of antimicrobials in different fields has created enormous pressure for the selection of resistance among opportunistic bacterial pathogen. one hundred four e. coli isolates were collected and identified from swine with diarrhea in korea during the period of 2002. the isolates showed highly resistant to streptomycin (99.0%), tetracycline (97.1%), neomycin (91.3%) and carbenicillin (84.6%) in antimicrobial susceptibility test. moreover, all of the isolates showed multiple antimi ... | 2005 | 16131822 |
| macrolides and lincomycin susceptibility of mycoplasma hyorhinis and variable mutation of domain ii and v in 23s ribosomal rna. | a total of 151 strains of mycoplasma hyorhinis isolated from porcine lung lesions (weaned pigs, n=71, and finishers, n=80) were investigated for their in vitro susceptibility to 10 antimicrobial agents. thirty-one strains (28 from weaned pigs and 3 from finishers) showed resistance to 16-membered macrolide antibiotics and lincomycin. the prevalence of the 16-membered macrolide-resistant m. hyorhinis strain in weaned pigs from japanese herds has approximately quadrupled in the past 10 years. seve ... | 2005 | 16141666 |
| nationwide microbiological baseline data collected by sponge sampling during 1997 and 1998 for cattle, swine, turkeys, and geese. | during 1997 and 1998, the u.s. food safety and inspection service completed nationwide microbiological baseline studies on four separate categories of livestock and poultry. data were collected by sponge-sampling techniques. these studies were designed to provide nationwide estimates of the prevalence of salmonella and prevalence and concentrations of escherichia coli in cattle (n = 1,881), swine (n = 2,127), turkeys (n = 1,396), and geese (n = 102) in establishments under federal inspection. sa ... | 2005 | 16161683 |
| expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic b subunit of escherichia coli heat-labile enterotoxin in tobacco plants. | the pentameric b subunit of escherichia coli heat-labile enterotoxin (ltb) can be used as an efficient mucosal carrier of either immunogenic or tolerogenic t-cell epitopes. co-delivery of therapeutic proteins with carrier proteins could increase the effectiveness of the antigen. this paper reports the ability of transgenic tobacco plants to express a fusion protein consisting of the synthetic ltb and a synthetic neutralizing epitope of porcine epidemic diarrhea virus (pedv), causing an enteric d ... | 2006 | 16174563 |