Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| chemical composition, antibacterial and antifungal activities of flowerhead and root essential oils of santolina chamaecyparissus l., growing wild in tunisia. | the antimicrobial properties of essential oil from various santolina species have not been investigated enough in the previous studies dealing with the biological activities of medicinal plants. in tunisia, santolina chamaecyparissus l. (asteraceae) is the only santolina species recorded and is used as vermifuge and emmenagogue. the chemical composition, antibacterial and antifungal properties of essential oils from the flowerheads and roots of spontaneous s. chamaecyparissus growing in tunisia ... | 2017 | 28490960 |
| evaluation of the synergistic effect of tomatidine with several antibiotics against standard and clinical isolates of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. | antibiotic resistance is an important problem in antibiotic treatment of infections, particularly in hospitals. tomatidine is a plant secondary metabolite with antimicrobial and antifungal effects. this study examined the possible synergistic effect tomatidine with several antibiotics against standard and clinical strains of staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and escherichia coli. after determining the minimum inhibitory concentrations (mics) of antibiotics and ... | 2017 | 28496482 |
| antimicrobial resistance patterns in women with positive urine culture: does menopausal status make a significant difference? | aim. urinary tract infection (uti) is considered one of the most common bacterial infections in women. the aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated uti. methods. urinary samples (n = 4453) collected from women with uti were analyzed in terms of uropathogens present. these were considered as positive if bacterial growth wa ... | 2017 | 28497048 |
| etiological and resistance profile of bacteria involved in urinary tract infections in young children. | background. the objective of this study was to identify the bacteria most frequently responsible for urinary tract infection (uti) in the population of under-2-year-olds in our geographic area and to evaluate the activity of antibiotics widely used for uti treatment during a 4-year study period. materials and methods. a retrospective analysis was conducted of data on the identification and susceptibility of microorganisms isolated in urine samples from children under 2 years of age. results. a t ... | 2017 | 28497052 |
| inhibition of clostridium difficile in mice using a mixture of potential probiotic strains enterococcus faecalis nm815, e. faecalis nm915, and e. faecium nm1015: novel candidates to control c. difficile infection (cdi). | this study is aimed at the isolation, identification, and characterization of potential probiotic strains capable of inhibiting clostridium difficile in vitro and in vivo. twenty isolates were isolated from infant fecal samples and screened against c. difficile using their cell-free supernatant. only three isolates showed maximum inhibition from 56.05 to 60.60%, thus they were characterized for probiotic properties and safety. the results obtained approved their tolerance to the gastrointestinal ... | 2017 | 28497217 |
| an iterative glycosyltransferase ents catalyzes transfer and extension of o- and s-linked monosaccharide in enterocin 96. | glycosyltransferases are essential tools for in vitro-glycoengineering. bacteria harbor an unexplored variety of protein glycosyltransferases. here, we describe a peptide glycosyltransferase (ents) encoded by orf0417 of enterococcus faecalis tx0104. ents di-glycosylates linear peptide of enterocin 96- a known antibacterial, in vitro. it is capable of transferring as well as extending the glycan onto the peptide in an iterative sequential dissociative manner. it can catalyze multiple linkages: gl ... | 2017 | 28498962 |
| occurrence of ampicillin-resistant enterococcus faecium carrying esp gene in pet animals: an upcoming threat for pet lovers. | this study was carried out to investigate oral colonisation by enterococcus faecalis and enterococcus faecium in pet dogs and cats, with special reference to antibiotic resistance. | 2017 | 28499907 |
| metabolic impact of nutrient starvation in mevalonate-producing escherichia coli. | the aim of this work was to enhance mevalonate yield from glucose in escherichia coli by essential nutrient starvations and to reveal these effects on the central carbon metabolism. stationary phase culture without essential nutrients such as nitrogen, sulfur, and magnesium was evaluated using an engineered e. coli introducing mvae and mvas genes from enterococcus faecalis. sulfur starvation resulted in the highest mevalonate yield of 0.61c-molc-mol(-1) from glucose. the metabolic impacts of nut ... | 2017 | 28501379 |
| chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae). | we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ... | 2017 | 28504841 |
| empyema thoracis caused by an optra-positive and linezolid-intermediate enterococcus faecalis strain. | 2017 | 28506787 | |
| microbiological analysis of acute infections of the nail fold on the basis of bait thread test. | an acute infection of the nail fold, called paronychia, is a common clinical problem. the basis for the implementation of the treatment is the result of microbiological examination. due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. | 2017 | 28507488 |
| application of hyperosmotic nanoemulsions in wound healing: partial thickness injury model in swine. | objective: in this work, we introduce a novel hyperosmotic nanoemulsion (hne) topical agent for use in wound healing. these topical emulsion complexes combine a lipophilic thymol nanoemulsion with a hyperosmotic saccharide matrix. this combination has been previously shown to possess synergistic antimicrobial activity against a host of common and drug-resistant pathogens in vitro. approach: in this study, we present additional data to assess the safety and efficacy of these emulsions in a partia ... | 2017 | 28507786 |
| the effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model. | to investigate the effect of sodium hypochlorite concentration and needle extension on removal of enterococcus faecalis biofilm, sixty root canal models were 3d printed. biofilms were grown on the apical 3 mm of the canal for 10 days. irrigation for 60s with 9 ml of either 5.25% or 2.5% naocl or water was performed using a needle inserted either 3 or 2 mm from the canal terminus and imaged using fluorescence microscopy and residual biofilm percentages were calculated using imaging software. the ... | 2017 | 28508523 |
| antimicrobial, anticancer, and antioxidant compounds from premna resinosa growing in saudi arabia. | premna resinosa (hochst.) schauer (lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. | 2017 | 28508699 |
| anti-inflammatory and regenerative effects of albanian propolis in experimental vital amputations. | this study evaluated the effects of albanian propolis on the inflamed pulpal tissue after pulpotomy in piglets. in five piglets, two teeth each were infected using special pathogenic microbial flora that was prepared in advance in order to cause inflammation of the pulp. pulpotomy was performed in the maxillary and mandibular central and lateral incisors. microbial flora pathogenesis prepared from the section of infected teeth, containing staphylococcus aureus, streptococcus viridans, enterococc ... | 2016 | 28509506 |
| development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens. | to develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (rpa) assays for detection of common bacterial urinary tract infection (uti) pathogens. | 2017 | 28510991 |
| rapid identification of vancomycin resistant enterococcus faecalis clinical isolates using a sugar fermentation method. | vancomycin resistant enterococci (vre) can be found all over the world. thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. | 2017 | 28511382 |
| comparative evaluation of three herbal solutions on the disinfection of guttapercha cones: an in vitro study. | guttapercha (gp) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. supplementary decontamination of gp cones is critical. as they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. | 2017 | 28511502 |
| comparative antimicrobial efficacy of eucalyptus galbie and myrtus communis l. extracts, chlorhexidine and sodium hypochlorite against enterococcus faecalis. | the aim of this study was to evaluate the antimicrobial effect of eucalyptusgalbie and myrtus communis l. methanolic extracts, chlorhexidine (chx) and sodium hypochlorite (naocl) on enterococcus faecalis (e. faecalis) as the predominant species isolated from infected root canals. | 2017 | 28512487 |
| comparison of simplistic biofilm models for evaluating irrigating solutions. | many innovations have recently been implemented in the field of endodontics, often based on a variety of in-vitro/ex-vivo test setups. it was the goal of this research to compare different biofilm models for evaluating the effectiveness of rinsing solutions. | 2017 | 28512652 |
| water and air ozone treatment as an alternative sanitizing technology. | we investigated the effectiveness of ozone (aqueous and gaseous) treatment as an alternative sanitizing technology to common conventional disinfectants in reducing the microbial contamination of both water and air. | 2017 | 28515631 |
| mechanisms of peptide sex pheromone regulation of conjugation in enterococcus faecalis. | in many gram positive bacteria, horizontal transfer and virulence are regulated by peptide-mediated cell-cell signaling. the heptapeptide ccf10 (c) activates conjugative transfer of the enterococcus faecalis plasmid pcf10, whereas the icf10 (i) peptide inhibits transfer. both peptides bind to the same domain of the master transcription regulator prgx, a repressor of transcription of the prgq operon encoding conjugation genes. we show that repression of prgq by prgx tetramers requires formation o ... | 2017 | 28523739 |
| occurrence of enterococcus faecalis and enterococcus faecium in various clinical infections: detection of their drug resistance and virulence determinants. | the aim of this study was to characterize virulence determinants and antibiotic resistance profiles in enterococci obtained from various clinical sources in the northwest of iran. a total of 160 enterococcal clinical isolates from various wards of university teaching hospitals were collected and specified by biochemical test, from september 2014 to july 2015. identification of enterococci was confirmed by multiplex pcr in the genus and species level. antibiotic resistance properties and virulenc ... | 2017 | 28525287 |
| effect of gnp functionalisation and multiple n-methylation of β-amyloid residue (32-37) on gram-positive bacterium. | in the previous report, the authors showed the gold nanoparticle (gnp) functionalised multiple n-methylated fragments of the residue (32-37) of beta (β)-amyloid protein (1-42), cggiglmvg and cgggggiglmvg toward disruption of β-amyloid (1-42), the predominant component of senile plaques. herein the in vitro antimicrobial activities of both normal and multiple n-methylated sequences of cggiglmvg and cgggggiglmvg were screened and it was found that all the eight sequences including four (non-functi ... | 2017 | 28530185 |
| the bacteriophage ef-p29 efficiently protects against lethal vancomycin-resistant enterococcus faecalis and alleviates gut microbiota imbalance in a murine bacteremia model. | enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. treating e. faecalis infections has become increasingly difficult because of the prevalence of multidrug-resistant e. faecalis strains. because bacteriophages show specificity for their bacterial hosts, there has been a growth in interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infect ... | 2017 | 28536572 |
| emergence of vana enterococcus faecium in denmark, 2005-15. | to describe the changing epidemiology of vancomycin-resistant enterococcus faecium and enterococcus faecalis in clinical samples in denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical e. faecium isolates from 2015. | 2017 | 28541565 |
| antimicrobial resistance status of enterococcus from australian cattle populations at slaughter. | antimicrobial agents are used in cattle production systems for the prevention and control of bacterial associated diseases. a consequence of their use is the potential development of antimicrobial resistance (amr). enterococcus faecium and enterococcus faecalis that are resistant to antimicrobials are of increased concern to public health officials throughout the world as they may compromise the ability of various treatment regimens to control disease and infection in human medicine. australia i ... | 2017 | 28542602 |
| influence of different wild-garlic (allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. | as there are no previous studies of the european wild-garlic (allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. | 2017 | 28543032 |
| identification of enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity. | nitroreductases, nad(p)h dependent flavoenzymes, are found in most of bacterial species. even if enterococcus faecalis strains seems to present such activity because of their sensitivity to nitrofurans, no enzyme has been described. nitroreductases were separated of others reductases due to their capacity to reduce nitro compounds. they are further classified based on their preference in cofactor: nadh and/or nadph. however, recently, azoreductases have been studied for their strong activity on ... | 2017 | 28545445 |
| comparative evaluation of micron- and nano-sized intracanal medicaments on penetration and fracture resistance of root dentin - an in vitro study. | intracanal medicaments play a vital role in disinfection of root canal system. the aim of this study was to evaluate intratubular penetration and fracture resistance of roots filled with micron- and nano-sized intracanal medicaments: calcium hydroxide (ch), nanocalcium hydroxide (nch), chitosan (cs) and nanochitosan (ncs). their antibacterial effect on e. faecalis was tested using agar diffusion method. nch and ncs were prepared by precipitation method and ionic crosslinking respectively. nch an ... | 2017 | 28545965 |
| a novel polytetrafluoroethylene-channel model, which simulates low levels of culturable bacteria in buildup biofilm after repeated endoscope reprocessing. | clinical studies have shown variable culture results from flexible endoscope channels possibly because of low levels of bacteria that are difficult to extract. the aim of this study was to develop a simulated-use buildup biofilm (bbf) model that mimics low levels of viable bacteria after repeated rounds of aldehyde fixation and accumulation. | 2017 | 28551023 |
| structure and dimerization of ireb, a negative regulator of cephalosporin resistance in enterococcus faecalis. | enterococcus faecalis, a leading cause of hospital-acquired infections, exhibits intrinsic resistance to most cephalosporins, which are antibiotics in the beta-lactam family that target cell-wall biosynthesis. a comprehensive understanding of the underlying genetic and biochemical mechanisms of cephalosporin resistance in e. faecalis is lacking. we previously determined that a transmembrane serine/threonine kinase (irek) and its cognate phosphatase (irep) reciprocally regulate cephalosporin resi ... | 2017 | 28551334 |
| collaborative research on puerperal infections in bangladesh. | bangladesh is considered as a high-risk country for emerging infectious diseases because of its high population density, poverty, and unhygienic conditions. although control efforts have primarily been focused on major infectious diseases such as diarrheal diseases, tuberculosis, malaria, and hiv infection, the prevalence and impact of many local or minor infectious diseases are still unclarified in this country. in this review, we present our recent experience and outcomes of collaborative rese ... | 2017 | 28552890 |
| human bile reduces antimicrobial activity of selected antibiotics against enterococcus faecalis and escherichia coli in vitro. | it has been known from previous studies that body fluids, such as cerebrospinal fluid, lung surfactant, and urine, have a strong impact on the bacterial killing of many anti-infective agents. however, the influence of human bile on the antimicrobial activity of antibiotics is widely unknown. human bile was obtained and pooled from 11 patients undergoing cholecystectomy. after sterilization of the bile fluid by gamma irradiation, its effect on bacterial killing was investigated for linezolid (lzd ... | 2017 | 28559254 |
| antimicrobial and anti-biofilm properties of polypropylene meshes coated with metal-containing dlc thin films. | a promising strategy to reduce nosocomial infections related to prosthetic meshes is the prevention of microbial colonization. to this aim, prosthetic meshes coated with antimicrobial thin films are proposed. commercial polypropylene meshes were coated with metal-containing diamond-like carbon (me-dlc) thin films by the magnetron sputtering technique. several dissimilar metals (silver, cobalt, indium, tungsten, tin, aluminum, chromium, zinc, manganese, tantalum, and titanium) were tested and com ... | 2017 | 28560581 |
| virulence factors associated with enterococcus faecalis infective endocarditis: a mini review. | the enterococci are accountable for up to 20% of all cases of infective endocarditis, with enterococcus faecalis being the primary causative isolate. infective endocarditis is a life-threatening infection of the endocardium that results in the formation of vegetations. based on a literature review, this paper provides an overview of the virulence factors associated with e. faecalis infective endocarditis. furthermore, it reports the effects of active or passive immunization against some of these ... | 2017 | 28567146 |
| effects of diode laser, gaseous ozone, and medical dressings on enterococcus faecalis biofilms in the root canal ex vivo. | the objective was to compare the antibacterial effects of adjunctive disinfection using diode laser and gaseous ozone compared to the medical dressings calcium hydroxide (ca(oh)2) and chlorhexidine gel (chx-gel) on enterococcus faecalis biofilms in human root canals ex vivo. root canals of 180 human extracted teeth were infected by e. faecalis and divided into 3 main groups (g): g1, control; g2, instrumentation and irrigation using 0.9% nacl; g3, instrumentation and irrigation using 1% naocl. in ... | 2017 | 28567421 |
| formulation and evaluation of sol-gel drug delivery system for intracanal ph sensitive controlled delivery of chlorhexidine. | persistence of viable micro-organisms even after thorough chemomechanical debridement has been cited as a major cause for endodontic failure. chlorhexidine is a drug, which has shown marked efficacy against enterococcus faecalis and candida spp., which are mostly accounted for endodontic failure and it has demonstrated high degree of substantivity to dentin by adsorption. another issue with chlorhexidine and other intracanal medicaments is the excessive or premature peaking of the drug leading t ... | 2017 | 28571266 |
| comparative evaluation of different modes of laser assisted endodontics in primary teeth: an in vitro study. | the presence of bacteria in root canals has been considered to be responsible for endodontic treatment failure, even in case of primary teeth. the use of lasers can be a valuable addition in removing bacterial load in areas where traditional methods may fail to succeed. methods like direct laser irradiation of canals, laser activated irrigation (lai) with sodium hypochlorite (naocl) and photodynamic therapy (pdt) seem to be a promising alternative for disinfection. | 2017 | 28571279 |
| phytochemical characterisation and bioprospection for antibacterial and antioxidant activities of lippia alba brown ex britton & wilson (verbenaceae). | ethanol extract and fractions obtained from fresh and dry aerial parts of lippia alba were examined in order to determine their phytochemical composition, antioxidant capacity and antibacterial activities. the ethanol extracts and fractions exhibited an antioxidant effect by the dpph assay, especially samples of fresh plant. hplc analysis of the ethyl acetate fractions identified the presence of phenolic acids and flavonoids. the ethanol extract and fractions showed activity against reference an ... | 2017 | 28571495 |
| detection of the optra gene in a clinical st16 enterococcus faecalis isolate in denmark. | 2017 | 28572038 | |
| bactericidal activity and synergy studies of peptide ap-cect7121 against multi-resistant bacteria isolated from human and animal soft tissue infections. | ap-cect7121 is an antimicrobial peptide, produced by enterococcus faecalis cect7121, with bactericidal activity against gram-positive bacteria. the aim of this study was to evaluate the bactericidal activity of ap-cect7121, alone and with gentamicin, against multi-resistant bacteria isolated from human and animals with soft tissue infections. during the period 2014-2015, bacterial strains producing human and animal soft tissue infections were studied. samples from patients attended at a general ... | 2017 | 28573492 |
| analysis of the incidence and risk factors of male urinary tract infection following urodynamic study. | the purpose of this study was to investigate the incidence and risk factors of male urinary tract infection (uti) after urodynamic study (uds). a total of 854 consecutive male patients, who underwent uds at peking union medical college hospital from january 2010 to march 2016, were recruited in this study. two to four weeks before the examination, urinalysis with bacterial culture was performed. patients with negative results were selected for uds. immediately before the examination, urinalysis ... | 2017 | 28577157 |
| genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance enterococcus faecalis isolated from blood culture. | enterococcus spp. is the common of intestinal micro flora in humans but nowadays this gram-positive bacterium causes the variety of nosocomial infections. resistance to antibiotic and also, presence of different virulence genes in the enterococcus spp. can change it to problematic microorganisms in the health care centers. the aim of this study was determined the genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance enterococcus faecalis isolated from b ... | 2017 | 28578090 |
| antibacterial properties of chitosan nanoparticles and propolis associated with calcium hydroxide against single- and multispecies biofilms: an in vitro and in situ study. | the aim of this study was to evaluate the efficacy of chitosan nanoparticles (cnps) and ethanolic propolis extract (epe) incorporated into a calcium hydroxide paste (ca[oh]2) to kill bacterial biofilms. | 2017 | 28578886 |
| the role of reactive oxygen species in the antimicrobial activity of pyochelin. | the increase in prevalence of antimicrobial-resistant bacteria (arb) is currently a serious threat, thus there is a need for new antimicrobial compounds to combat infections caused by these arb. an antimicrobial-producing bacterium, burkholderia paludis was recently isolated and was able to produce a type of siderophore with antimicrobial properties, later identified as pyochelin. the chelating ability of pyochelin has been well-characterized but not for its antimicrobial characteristics. it was ... | 2017 | 28580180 |
| can the enterococcus faecalis identified in the root canals of primary teeth be a cause of failure of endodontic treatment? | this study investigated the presence of enterococcus faecalis in primary teeth with primary root canal infections and related to the possible failure of pulpectomy outcome after 36 months. | 2017 | 28580816 |
| implementation of the new virtuo blood culture system: evaluation and comparison to the 3d system using simulated blood cultures. | to evaluate the performances of the newly approved bact/alert virtuo blood culture system for the recovery of bloodstream pathogens and compare it to the bact/alert 3d system. | 2017 | 28583022 |
| susceptibility pattern of enterococci at tertiary care hospital. | the study was aimed to characterize enterococci from various clinical specimens, to determine the antimicrobial susceptibility pattern, and to explore the association between virulence factors and antimicrobial resistance. a total of 283 clinical enterococcal isolates were speciated and subjected to antimicrobial susceptibility testing. virulence factors (hemolysin, gelatinase, and biofilm production) were detected phenotypically. of the 283 enterococci isolated, 12 species were identified; pred ... | 2017 | 28584459 |
| 2,6-difluorobenzamide inhibitors of bacterial cell division protein ftsz: design, synthesis, and structure-activity relationships. | a wide variety of drug-resistant microorganisms are continuously emerging, restricting the therapeutic options for common bacterial infections. antimicrobial agents that were originally potent are now no longer helpful, due to their weak or null activity toward these antibiotic-resistant bacteria. in addition, none of the recently approved antibiotics affect innovative targets, resulting in a need for novel drugs with innovative antibacterial mechanisms of action. the essential cell division pro ... | 2017 | 28586174 |
| evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms. | scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus). | 2017 | 28587870 |
| an entirely solid phase peptide synthesis-based strategy for synthesis of gelatinase biosynthesis-activating pheromone (gbap) analogue libraries: investigating the structure-activity relationships of the enterococcus faecalis quorum sensing signal. | the development of an entirely solid-phase peptide synthesis (spps)-based synthesis of the quorum sensing signal gelatinase biosynthesis-activating pheromone (gbap) from enterococcus faecalis is reported. the method was used to prepare three libraries of analogues to investigate the structure-activity relationships (sars) of the gbap signal. the sar studies revealed new characteristics of the gbap signal and uncovered the most potent quorum sensing activator in e. faecalis known to date. | 2017 | 28590764 |
| a loop-mediated isothermal amplification (lamp) assay for the rapid detection of enterococcus spp. in water. | faecal pollution of water and the resulting potential presence of human enteric pathogens is a predominant threat to public health. microbiological water quality can be assessed by the detection of standard faecal indicator bacteria (sfib) such as e. coli or certain enterococcus species. in recent years, isothermal amplification methods have become a useful alternative to polymerase chain reaction (pcr), allowing molecular diagnostics with simple or no instrumentation. in this study, a novel scr ... | 2017 | 28591662 |
| antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi. | lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. the main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. caloplaca pusilla, protoparmeliopsis muralis and xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone ex ... | 2017 | 28592323 |
| antibiotic resistance genes and antibiotic susceptibility of oral enterococcus faecalis isolates compared to isolates from hospitalized patients and food. | enterococcus faecalis, a commensal of the intestinal tract of humans and animals is of great significance as leading opportunistic pathogen, and also prevalent in oral diseases, such as endodontic infections, as well as the healthy oral cavity. to investigate the potential of oral e. faecalis to constitute a reservoir of antibiotic resistance, isolates from supragingival plaque/saliva and from endodontic infections were screened regarding their resistance to selected antibiotics in comparison to ... | 2017 | 28601926 |
| a new epipolythiodioxopiperazine with antibacterial and cytotoxic activities from the endophytic fungus chaetomium sp. m336. | a new compound 6-formamide-chetomin (1) together with chetomin (2) was isolated from solid fermentation products of the endophytic fungus chaetomium sp. m336, which were elucidated by extensive spectroscopic analyses, including 1d and 2d nmr, and hr-esi-ms experiments. the bioassay result showed that compound 1 had strong antibacterial activity against escherichia coli, staphylococcus aureus, salmonella typhimurium atcc 6539 and enterococcus faecalis with minimum inhibitory concentration (mic) v ... | 2017 | 28602097 |
| patterns of multi-drug resistant bacteria at first culture from patients admitted to a third level university hospital in calabria from 2011 to 2014: implications for empirical therapy and infection control. | surveillance of antimicrobial drug resistance is fundamental to guide empirical treatment. however, the european antimicrobial resistance surveillance network provides a general picture, which might not be applicable to clinical settings that are excluded from this survey. we evaluated resistance patterns of eskape isolates over a four-year period in a third level university hospital in the province of catanzaro (southern italy). in this retrospective study, we evaluated the frequency of eskape ... | 2017 | 28603227 |
| molecular epidemiology and antibiotic resistance patterns of enterococcus faecalis isolates from hospitals in tehran. | enterococcus faecalis is one of the most important causative agents of nosocomial infections. control and prevention of these infections require appropriate epidemiological knowledge. this study investigated the correlation between molecular characteristics and drug resistance of e. faecalis isolates from local hospitals. a total of 125 isolates of enterococcus faecalis from two hospitals in tehran were identified by using culture and biochemical method. an antibiotic resistance assay was carrie ... | 2017 | 28603229 |
| a pilot-scale microwave technology for sludge sanitization and drying. | large volumes of sludge are produced from onsite sanitation systems in densely populated areas (e.g. slums and emergency settlements) and wastewater treatment facilities that contain high amounts of pathogens. there is a need for technological options which can effectively treat the rapidly accumulating sludge under these conditions. this study explored a pilot-scale microwave (mw) based reactor as a possible alternative for rapid sludge treatment. the reactor performance was examined by conduct ... | 2017 | 28605862 |
| bacterial sepsis in neonates: single centre study in a neonatal intensive care unit in bosnia and herzegovina. | the aim of the study was to evaluate the incidence, mortality, risk factors, aetiology and the susceptibility to antibiotics of the bacteria responsible for sepsis. | 2017 | 28605923 |
| signal biosynthesis inhibition with ambuic acid as a strategy to target antibiotic-resistant infections. | there has been major interest by the scientific community in antivirulence approaches against bacterial infections. however, partly due to a lack of viable lead compounds, antivirulence therapeutics have yet to reach the clinic. here we investigate the development of an antivirulence lead targeting quorum sensing signal biosynthesis, a process that is conserved in gram-positive bacterial pathogens. some preliminary studies suggest that the small molecule ambuic acid is a signal biosynthesis inhi ... | 2017 | 28607020 |
| in vitro evaluation of antimicrobial activity of alimentary canal extracts from the red palm weevil, rhynchophorus ferrugineus olivier larvae. | the invasive red palm weevil, rhynchophorus ferrugineus olivier (coleoptera: curculionidae), is considered one of the world's most devastating insect pests to palm trees. it was observed that larvae of this pest are able to inhibit microbial growth on the rearing media when they start feeding and this observation has led us to study the effect of red palm weevils on various microbial species. the antimicrobial effect of extracts from different parts of the alimentary canal on gram positive bacte ... | 2017 | 28612029 |
| cytotoxic and antimicrobial activities of cantharellus cibarius fr. (cantarellaceae). | antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of cantharellus cibarius were tested. broth microdilution assay was performed against 10 bacterial strains (staphylococcus aureus, s. epidermidis, micrococcus luteus, bacillus subtilis, enterococcus feacalis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella abony), with emphasis on helicobacter pylori. methanol extract was the most active against h. pylori strains wit ... | 2017 | 28613963 |
| triterpenoid saponins and other glycosides from the stems and bark of jaffrea xerocarpa and their biological activity. | six previously undescribed triterpenoid saponins and two previously undescribed norlupane triterpenes were isolated with five known saponins, three known lupane derivatives, 17,20-didehydro-20-deoxyjujubogenin, rutin, (±) 3α-o-β-d-glucopyranosyl-lyoniresinol, (±) 4-o-β-d-glucopyranosyl-maesopsin, three phenol glycosides, and uridine from the stems and bark of jaffrea xerocarpa (baill.) h. c. hopkins & pillon (= basionym alphitonia xerocarpus baill.) (rhamnaceae), an endemic tree of new caledonia ... | 2017 | 28614728 |
| characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications. | the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ... | 2017 | 28617525 |
| evaluation of antimicrobial activity of association of chlorhexidine to photosensitizer used in photodynamic therapy in root canals infected by enterococcus faecalis. | the aim of the present study was to evaluate, in vitro, the influence of the addition of chlorhexidine to photosensitiser in the antimicrobial activity of photodynamic therapy in root canals infected by enterococcus faecalis. | 2017 | 28619615 |
| draft genome sequences of six enterococcus faecalis strains isolated from malaysian clinical and environmental origins. | enterococcus faecalis is known to cause a variety of nosocomial infections, including urinary tract infections. antibiotic resistance and virulence properties in this species are of public concern. the draft genome sequences of six e. faecalis strains isolated from clinical and environmental sources in malaysia are presented here. | 2017 | 28619812 |
| infectious crystalline keratopathy after descemet’s stripping endothelial keratoplasty. | a 68-year-old woman presented with infectious crystalline keratopathy 4 months after she underwent a combined phacoemulsification and descemet's stripping endothelial keratoplasty for fuch's endothelial dystrophy in her left eye. after 5 months of topical moxifloxacin 1%, the infiltrate responded well but had not completely resolved, with the resulting endothelial failure requiring a penetrating keratoplasty 9 months after the initial operation. microbiology identified enterococcus faecalis with ... | 2017 | 28619977 |
| photodvnamic therapy in endodontics. | commonly used irrigants do not always eradicate the entire microbial flora in infected root canals. therefore, several other strategies, such as photodynamic therapy (pdt) have been developed. photoactivated disinfection is based on the interaction of a photosensitive antibacterial agent and a light source. it uses a nontoxic dye named photosensitizer (ps) and low-intensity visible light. in oxygen presentation, these combine to produce some cytotoxic species. the ps molecules attach to bacteria ... | 2017 | 28621288 |
| spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary. | surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time. | 2017 | 28621642 |
| design, synthesis, and evaluation of novel tyrosine-based dna gyrase b inhibitors. | the discovery and synthesis of new tyrosine-based inhibitors of dna gyrase b (gyrb), which target its atpase subunit, is reported. twenty-four compounds were synthesized and evaluated for activity against dna gyrase and dna topoisomerase iv. the antibacterial properties of selected gyrb inhibitors were demonstrated by their activity against staphylococcus aureus and enterococcus faecalis in the low micromolar range. the most promising compounds, 8a and 13e, inhibited escherichia coli and s. aure ... | 2017 | 28621824 |
| bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation against biofilms of enterococcus faecalis isolate from canal of root-filled teeth with periapical lesions. | this study was to evaluate the bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation (er:yag + naocl) on biofilms of enterococcus faecalis clinical isolate. | 2017 | 28622484 |
| molecular identification of multiple antibiotic resistant fish pathogenic enterococcus faecalis and their control by medicinal herbs. | the opportunistic fish pathogen, enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. the objectives of this study were to (i) identify e. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of e. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. a total of 48 isolates were phenotypically identified as enterococcus species ... | 2017 | 28623336 |
| high level bacterial contamination of secondary school students' mobile phones. | while contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones. | 2017 | 28626737 |
| inactivation of bacterial biofilms by visible light-activated unmodified zno nanorods. | various zinc oxide (zno) nanostructures are widely used for photocatalytic antibacterial applications. since zno possess a wide bandgap, it is believed that only uv light may efficiently assist bacterial inactivation and diverse crystal lattice modifications should be applied in order to narrow the bandgap for efficient visible light absorption. in this work we show that even unmodified zno nanorods grown by aqueous chemical growth technique were found to possess intrinsic defects which can be a ... | 2017 | 28628486 |
| antibacterial activity of crude extracts of some south african medicinal plants against multidrug resistant etiological agents of diarrhoea. | this study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the eastern cape province, south africa. | 2017 | 28629407 |
| telavancin: a novel semisynthetic lipoglycopeptide agent to counter the challenge of resistant gram-positive pathogens. | telavancin (td-6424), a semisynthetic lipoglycopeptide vancomycin-derivative, is a novel antimicrobial agent developed by theravance for overcoming resistant gram-positive bacterial infections, specifically methicillin-resistant staphylococcus aureus (mrsa). the us food and drug administration (usfda) had approved telavancin in 2009 for the treatment of complicated skin and skin structure infections (csssis) caused by gram-positive bacteria, including mrsa (s. aureus, streptococcus agalactiae, s ... | 2017 | 28634536 |
| the curious case of achromobacter eurydice, a gram-variable pleomorphic bacterium associated with european foulbrood disease in honeybees. | honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. bacterial pathogens in particular lead to destructive infections of the brood. european foulbrood is caused by the bacterium melissococcus plutonius in combination with several other gram-positive bacteria (achromobacter eurydice, bacillus pumilus, brevibacillus laterosporus, enterococcus faecalis, paenibacillus alvei, paenibacillus dendritiformis) involved as secondary invaders following the in ... | 2017 | 28634639 |
| antibacterial activity of different root canal sealers against enterococcus faecalis. | the aim of the present study was to compare in vitro the antimicrobial activity of different root canal sealers against enterococcus faecalis, prior and subsequent to setting. | 2017 | 28638549 |
| comparative evaluation of anti microbial effects of triple antibiotic paste and amox and its derivatives against e. faecalis: an in vitro study. | enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. triple antibiotic paste has stood the test of time as a proven antibiotic combination against e. faecalis. however, problems with this include staining of teeth and standardization of the preparation. thus, the search for better alternatives and better preparation techniques is still on. | 2017 | 28638559 |
| phytochemical screening, antimicrobial and cytotoxicity studies of ethanol leaf extract of aphaniasenegalensis (sapindaceae). | aphania senegalensis (sapindaceae) is commonly used in senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections. the aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens. | 2017 | 28638876 |
| 1,1ꞌ-carbonyldiimidazole (cdi) mediated facile synthesis, structural characterization, antimicrobial activity and in-silico studies of coumarin-3-carboxamide derivatives. | despite of the availability of variety of antibacterial agents, re-emergance of pathogenic bacteria is still a serious medical concern. so, identification of new, safer, and selective antibacterial agents is the key interest in the medicinal chemistry research. | 2017 | 28641525 |
| the effect of gelatinase production of enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants. | objectives: the aim of this study was to evaluate whether the gelatinase production ability of enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. materials and methods: standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine (chx), group 3: naocl + saline + chx, group 4: naocl + edta + naocl, group 5: qmix. after incubati ... | 2016 | 28642925 |
| exploiting crispr-cas to manipulate enterococcus faecalis populations. | crispr-cas provides a barrier to horizontal gene transfer in prokaryotes. it was previously observed that functional crispr-cas systems are absent from multidrug-resistant (mdr) enterococcus faecalis, which only possess an orphan crispr locus, termed crispr2, lacking cas genes. here, we investigate how the interplay between crispr-cas genome defense and antibiotic selection for mobile genetic elements shapes in vitro e. faecalis populations. we demonstrate that crispr2 can be reactivated for gen ... | 2017 | 28644125 |
| methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate. | the aim of this study was to develop and to characterize a methacrylate-based root canal sealer containing chlorhexidine (chx) and α-tricalcium phosphate (α-tcp). experimental dual cure methacrylate-based sealer was produced containing 0, 2.5, or 5 wt% of chx and 0, 25, or 50 wt% of α-tcp. experimental sealers were evaluated based on flow, film thickness, radiopacity, degree of conversion (dc), degradation in water, ph and antibacterial activity. flow ranged from 15.09 ± 0.11 to 17.47 ± 0.42 mm. ... | 2017 | 28644564 |
| phtβ-promoted mobilization of non-conjugative resistance plasmids from enterococcus faecium to enterococcus faecalis. | to analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, prum17i48 and plag (formerly named pdo1-like), from enterococcus faecium 17i48 to enterococcus faecalis jh2-2. | 2017 | 28645197 |
| antibacterial effect of genetically-engineered bacteriophage ϕef11/ϕfl1c(δ36)p(nisa) on dentin infected with antibiotic-resistant enterococcus faecalis. | enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. e. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. different e. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic ... | 2017 | 28646693 |
| [mitral valve endocarditis diagnosed after panophthalmitis with enterococcus faecalis]. | an 86-year-old man underwent cataract surgery on the left eye, but suddenly he lost his vision three days after surgery. topical treatments for panophthalmitis were started according to international guidelines. later, a broad-range polymerase chain reaction showed enterococcus faecalis in corpus vitreum, and iv antiobiotic treatment was initiated. to exclude endocarditis an echocardiography was performed, and this showed mitral regurgitation with a small mobile vegetation. uncertainty concernin ... | 2017 | 28648168 |
| altered antibacterial activity of curcumin in the presence of serum albumin, plasma and whole blood. | antibacterial effect is one of the major therapeutic activities of plant-derived curcumin. this work evaluated the effect of serum albumin, human plasma, and whole blood on the in vitro activity of curcumin against eight clinical bacterial isolates by standard broth microdilution and plate-counting methods. toxicological effects of curcumin towards human red blood cells (rbcs) and peripheral blood mononuclear cells (pbmcs) were also investigated. curcumin exhibited weak activity against gram-neg ... | 2017 | 28649069 |
| comparison of the antimicrobial efficacy of calcium hydroxide and photodynamic therapy against enterococcus faecalis and candida albicans in teeth with periapical lesions; an in vivo study. | introduction: elimination of pathological microflora of root canal systems is a major goal in endodontic treatment. this study aimed to compare the antimicrobial efficacy of calcium hydroxide as an intracanal medication and antibacterial photodynamic therapy (apdt) against enterococcus faecalis and candida albicans in teeth with periapical (pa) lesions. methods: this in vivo study was conducted on 20 patients with single-rooted mandibular premolar with previously failed endodontic treatment. thi ... | 2017 | 28652899 |
| urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients. | background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ... | 2017 | 28663538 |
| screening of aqueous extracts of medicinal herbs for antimicrobial activity against oral bacteria. | dental caries is considered to be a preventable disease, and various antimicrobial agents have been developed for the prevention of dental diseases; however, many bacteria show resistance to existing agents. in this study, 14 medicinal herbs were evaluated for antimicrobial activity against five common oral bacteria as a screen for potential candidates for the development of natural antibiotics. | 2013 | 28664049 |
| monitoring in vitro antibacterial efficacy of 26 indian spices against multidrug resistant urinary tract infecting bacteria. | to screen methanolic extracts of 26 commonly used indian spices against nine species of uropathogenic bacteria (enterococcus faecalis, staphylococcus aureus, acinetobacter baumannii, citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa), isolated from clinical samples of a tertiary care hospital for antibacterial activity. | 2014 | 28664089 |
| microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure. | the quantification of ten microorganisms at the root ends and in the surrounding periradicular lesions was performed. thirty 3 mm samples root ends and 30 samples of the surrounding chronic periapical infection were collected during apical microsurgery. samples were triturated, and the bacterial dna was obtained. the bacterial quantification was performed by using the sybr green system. at least one microorganism was detected in all patients. in both the root end and periapical samples, fusobact ... | 2017 | 28666877 |
| determining the optimal dose of 1940-nm thulium fiber laser for assisting the endodontic treatment. | insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. the optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm thulium fiber laser, were firstly investiga ... | 2017 | 28669067 |
| antibiotic activity of emerimicin iv isolated from emericellopsis minima from talcahuano bay, chile. | due to the increasing emergence of resistance of bacterial pathogens to current antibiotics, we have examined the marine fungi present in sea sediments obtained 200 m offshore to discover new antibacterial compounds active against multidrug-resistant bacteria. one strain, identified as emericellopsis minima, was isolated from sediments of talcahuano bay (chile). from the liquid culture of e. minima, we isolated emerimicin iv, a unique fungal peptaibol that exhibited antibacterial activity. the s ... | 2017 | 28669240 |
| prevalence and genetic diversity of enterococcus faecalis isolates from mineral water and spring water in china. | enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. the aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of e. faecalis from mineral water and spring water in china. of 314 water samples collected from january 2013 to january 2014, 48 samples (15.3%) were contami ... | 2017 | 28670302 |
| carriage of multidrug resistant enterococcus faecium and enterococcus faecalis among apparently healthy humans. | enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. recently, interest in two major species, e. faecium and e. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. this study was aimed at determining the prevalence of e. faecium and e. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics. | 2017 | 28670644 |
| stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells. | in enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. to better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (hcr) methodology for simultaneous quantification of multiple e. faecalis transcripts at the single cell level. we present direct evidence for variability in the ... | 2017 | 28671948 |
| induction of cryptic and bioactive metabolites through natural dietary components in an endophytic fungus colletotrichum gloeosporioides (penz.) sacc. | grape skin and turmeric extracts having the major components resveratrol and curcumin, respectively, were used for the induction of cryptic and bioactive metabolites in an endophytic fungus colletotrichum gloeosporioides isolated from syzygium cumini. the increase in total amount of crude compounds in grape skin and turmeric extract treated cultures was 272.48 and 174.32%, respectively, compared to the untreated control. among six human pathogenic bacteria tested, the maximum inhibitory activity ... | 2017 | 28674526 |