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the role of magnesium, pyrophosphate, and their complexes as substrates and activators of the vacuolar h+-pumping inorganic pyrophosphatase (studies using ligand protection from covalent inhibitors).inhibitors preferentially and covalently reactive with cysteine, arginine, histidine, and carboxyl-containing residues were inhibitory to the plant vacuolar h+-transporting inorganic pyrophosphatase (h+-ppase) from vigna radiata (mung bean) and beta vulgaris (red beet), but hydrophobic compounds and those reactive with tyrosine and lysine were less effective. inhibition by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, phenylglyoxal, and n-ethylmaleimide was decreased in the presence of mg2+ or ...199612226285
role of the plasma membrane h+-atpase in k+ transport.the role of the plant plasma membrane h+-atpase in k+ uptake was examined using red beet (beta vulgaris l.) plasma membrane vesicles and a partially purified preparation of the red beet plasma membrane h+-atpase reconstituted in proteoliposomes and planar bilayers. for plasma membrane vesicles, atp-dependent k+ efflux was only partially inhibited by 100 [mu]m vanadate or 10 [mu]m carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. however, full inhibition of atp-dependent k+ efflux by these reag ...199612226357
insect control and dosage effects in transgenic canola containing a synthetic bacillus thuringiensis cryiac gene.zygotic hypocotyls of canola (brassica napus l.) cv oscar, cv westar, and the breeding line uga188-20b were transformed with a truncated synthetic bacillus thuringiensis insecticidal crystal protein gene (bt cryiac) under the control of the cauliflower mosaic virus 35s promoter using agrobacterium tumefaciens-mediated transformation. fifty-seven independently transformed lines were produced, containing 1 to 12 copies of the transgenes. a range of cry expressors was produced from 0 to 0.4% cry as ...199612226379
identification and characterization of an inducible nad(p)h dehydrogenase from red beetroot mitochondria.exogenous nadh oxidation of mitochondria isolated from red beetroots (beta vulgaris l.) increased dramatically upon slicing and aging the tissue. anion-exchange chromatography of soluble fractions derived by sonication from fresh and aged beetroot mitochondria yielded three nadh dehydrogenase activity peaks. the third peak from aged beetroot mitochondria was separated into two activities by blue-affinity chromatography. one of these (the unbound peak) readily oxidized dihydrolipoamide, whereas t ...199612226415
stomatal guard cells are totipotent.it has been successfully demonstrated, using epidermis explants of sugar beet (beta vulgaris l.), that stomatal guard cells retain full totipotent capacity. despite having one of the highest degrees of morphological adaptation and a unique physiological specialization, it is possible to induce a re-expression of full (embryogenic) genetic potential in these cells in situ by reversing their highly differentiated nature to produce regenerated plants via a callus stage. the importance of these find ...199612226426
[medical-biological evaluation of genetically-modified sugar beet line 77 (chemical composition and toxicologico-biochemical studies)].the rats were fed with the genetically modified sugar beet line 77 (monsanto ko, usa) 10 g/rat/day for 1 month. their blood, urea and liver were investigated to measure total protein and glucose levels, aminotransferase and alkaline phosphatase activities, ph, creatinine level as well as hepatic enzyme activity of the i and ii phases of xenobiotic metabolism and whole and non-sedimentated lysosomal enzyme activities and activity of antioxidant system.200212227013
[medico-genetic evaluation of sugar sand, obtained from genetically-modified sugar beet line 77 (toxicologico-biochemical studies)].the rats were fed with the suger from the genetically modified suger beet line 77 (monsanto ko, usa) 3 g/rat/day for 3 months. their blood, urea and liver were investigated to measure total protein and glucose levels, aminotransferase and alkaline phosphatase activities, ph, creatinine level as well as hepatic enzyme activity of the i and ii phases of henobiotic metabolism and whole and non-sedimentated lysosomal enzyme activities and activity of antioxidant system.200212227014
purification, characterization, and submitochondrial localization of a 58-kilodalton nad(p)h dehydrogenase.an nadh dehydrogenase activity from red beet (beta vulgaris l.) root mitochondria was purified to a 58-kd protein doublet. an immunologically related dehydrogenase was partially purified from maize (zea mays l. b73) mitochondria to a 58-kd protein doublet, a 45-kd protein, and a few other less prevalent proteins. polyclonal antibodies prepared against the 58-kd protein of red beet roots were found to immunoprecipitate the nad(p)h dehydrogenase activity. the antibodies cross-reacted to similar pr ...199512228370
characteristics of sucrose transport and sucrose-induced h+ transport on the tonoplast of red beet (beta vulgaris l.) storage tissue.sucrose-induced changes of the energization state of the red beet root (beta vulgaris l. ssp. conditiva) vacuolar membrane were observed with the fluorescent dyes 6-chloro-9-{[4-(diethylamino)- 1-methylbutyl]-amino}-2-methoxyacridine dihydrochloride, as a ph monitor, and 9-amino-6-chloro-2-methoxyacridine (acma). consequently, transient acidification of the surrounding suspension medium could be measured with a ph electrode. this signal was specific for suc and was not seen for sorbitol, mannito ...199512228372
carbon, nitrogen, and nutrient interactions in beta vulgaris l. as influenced by nitrogen source, no3- versus nh4+sugar beets (beta vulgaris l. cv f58-554h1) were grown hydroponically in a 16-h light, 8-h dark period (photosynthetic photon flux density of 0.5 mmol m-2 s-1) for 4 weeks from sowing in half-strength hoagland nutrient solution containing 7.5 mm nitrate. half of the plants were then transferred to 7.5 mm ammonium n; the rest remained in solution with 7.5 mm nitrate n. upon transfer from nitrate to ammonium, the total n concentration decreased sharply in the fibrous roots and petiole/midribs and ...199512228383
leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet (iv. changes with time following increased supply of phosphate to low-phosphate plants).changes in photosynthesis, carbon partitioning, and growth following resupply of orthophosphate (pi) to moderately p-deficient plants (low-p) were determined for sugar beets (beta vulgaris l. cv f58-554h1) cultured hydroponically in growth chambers. one set of plants was supplied with 1.0 mm pi in half-strength hoagland solution (control plants), and a second set (low-p plants) was supplied with 0.05 mm pi. at the end of 2 weeks, the low-p plants were resupplied with 1.0 mm pi. low-p plants rapi ...199512228438
computer-assisted identification of protoplasts responsible for rare division events reveals guard-cell totipotency.with the use of a computer-controlled microscope system to assist in the positioning and rapid relocation of large numbers of cultured cells, we were able to identify those protoplasts with the capacity to divide within a highly recalcitrant culture in which only a tiny fraction of the total population proceeds to produce viable microcalli. in the cultures used, comprising beta vulgaris l. (sugar beet) leaf protoplasts, it was confirmed that these cells can be recognized solely on the basis of m ...199512228442
characterization of the red beet plasma membrane h+-atpase reconstituted in a planar bilayer system.the transport activity of the red beet (beta vulgaris l.) plasma membrane h+-atpase was examined following reconstitution into a planar bilayer membrane. fusion of partially purified plasma membrane h+-atpase with the bilayer membrane was accomplished by perfusion of proteoliposomes against the bilayer under hypoosmotic conditions. following incorporation into the bilayer, an atp-dependent current was measured that demonstrated properties consistent with those of the plasma membrane h+-atpase. c ...199512228483
end-product control of carbon metabolism in culture-grown sugar beet plants (molecular and physiological evidence on accelerated leaf development and enhanced gene expression).sugar beet (beta vulgaris l.) seedlings were grown on media containing 90 to 300 mm sucrose or glucose. compared to controls, sugar-grown plants had higher growth rate, photosynthesis, and leaf sugar levels. the steady-state level of transcripts increased significantly for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) (rbcs) and the cytosolic fructose-1,6-bisphosphatase and moderately for the rubisco large subunit (rbcl). the transcript level of sucrose phosphate ...199512228569
purification and characterization of a novel aminopeptidase, plastidial alanine-aminopeptidase, from the cotyledons of etiolated sugar beet seedlings.during prolonged dark growth of sugar beet (beta vulgaris l.) seedlings, etioplasts, rapidly after the proplastid-etioplast transition, undergo a degenerative process characterized by ultrastructural modifications, protein loss, and the decrease of carotenoid and chlorophyll accumulation upon illumination. two plastidial aminopeptidase activities were identified as early markers of this degenerative process (a. el amrani, i. couee, j.-p. carde, j.-p. gaudillere, p. raymond [1994] plant physiolog ...199512228582
purification and light-dependent molecular modulation of the cytosolic fructose-1,6-bisphosphatase in sugarbeet leaves.cytosolic fructose-1,6-bisphosphatase (fbpase) was purified 472-fold from sugarbeet (beta vulgaris l.) leaves by ammonium sulfate fractionation, anion-exchange chromatography (deae sepharose), cation-exchange chromatography (s-sepharose), gel filtration (sephacryl s-300), and hydrophobic interaction chromatography (phenyl sepharose). the dissociated polypeptide (molecular mass of 37 kd) was used to generate polyclonal antibodies. western blot analysis revealed a single band that was identified a ...199312231665
investigation of the calcium-transporting atpases at the endoplasmic reticulum and plasma membrane of red beet (beta vulgaris).calcium-transporting atpases were compared in endoplasmic reticulum (er)- and plasma membrane-enriched fractions of red beet (beta vulgaris l.) storage tissue by measuring 45ca uptake and calcium-dependent phosphoenzyme formation. the plasma membrane fraction was prepared by aqueous two-phase partitioning of a microsomal fraction and collecting the upper phase. the er-enriched fraction was obtained by submitting a sucrose-gradient er-enriched fraction to aqueous two-phase partitioning and collec ...199312231844
oxidation of external nad(p)h by mitochondria from taproots and tissue cultures of sugar beet (beta vulgaris).the present study compares the exogenous nad(p)h oxidation and the membrane potential ([delta][psi]) generated in mitochondria isolated from different tissues of an important agricultural crop, sugar beet (beta vulgaris}. we observed that mitochondria from taproots, cold-stored taproots, and in vitro-grown tissue cultures contain a functional nadh dehydrogenase, whereas only those isolated from tissue cultures displayed a functional nad(p)h dehydrogenase. it is interesting that the nadh-dependen ...199312231847
immunological evidence for the existence of a carrier protein for sucrose transport in tonoplast vesicles from red beet (beta vulgaris l.) root storage tissue.monoclonal antibodies were raised in mice against a highly purified tonoplast fraction from isolated red beet (beta vulgaris l. ssp. conditiva) root vacuoles. positive hybridoma clones and sub-clones were identified by prescreening using an enzyme-linked immunosorbent assay (elisa) and by postscreening using a functional assay. this functional assay consisted of testing the impact of hybridoma supernatants and antibody-containing ascites fluids on basal and atp-stimulated sugar uptake in vacuole ...199312231863
calcium retrieval from vacuolar pools (characterization of a vacuolar calcium channel).voltage patch-clamp experiments at the whole-vacuole and single-channel levels were employed to study the retrieval of ca2+ from vacuoles into the cytoplasm in sugar beet cell (beta vulgaris l.) suspension cultures. channels allowing the movement of ca2+ out of the vacuole were identified at physiological conditions of ph, vacuolar membrane potential, and vacuole/cytoplasm ca2+ concentrations. the operation of the channel was voltage dependent and inositol-1,4,5-triphosphate insensitive and disp ...199312231891
aminomethylenediphosphonate: a potent type-specific inhibitor of both plant and phototrophic bacterial h+-pyrophosphatases.the suitability of different pyrophosphate (ppi) analogs as inhibitors of the vacuolar h+-translocating inorganic pyrophosphatase (v-ppase; ec 3.6.1.1) of tonoplast vesicles isolated from etiolated hypocotyls of vigna radiata was investigated. five 1,1-diphosphonates and imidodiphosphate were tested for their effects on substrate hydrolysis by the v-ppase at a substrate concentration corresponding to the km of the enzyme. the order of inhibitory potency (apparent inhibition constants, kiapp valu ...199412232069
a study of the effect of inhibitors of the animal sarcoplasmic/endoplasmic reticulum-type calcium pumps on the primary ca2+-atpases of red beet.the inhibitor sensitivity of the endoplasmic reticulum (er) and plasma membrane (pm) calcium pumps of red beet (beta vulgaris l.) were studied by measuring the atp-driven accumulation of 45ca2+ into isolated membrane vesicles. both transporters were strongly inhibited by 50 [mu]mol m-3 erythrosin b, but only by 50% in the presence of 100 mmol m-3 vanadate. a number of inhibitors considered to be specific for the sarcoplasmic reticulum (sr)/er-type calcium pump in animal cells were used to furthe ...199412232168
nitrogen source regulation of growth and photosynthesis in beta vulgaris l.sugar beets (beta vulgaris l. cv f58-554h1) were grown hydroponically in a 16-h light, 8-h dark period at a photosynthetic photon flux density of 0.5 mmol m-2 s-1 for 4 weeks in half-hoagland culture solution containing only nitrate-nitrogen. half of the plants were then transferred to half-hoagland solution with ammonium-nitrogen (7.35mm), while the other half continued on 7.5 mm nitrate. growth analysis was carried out by sampling the plants at 3-d intervals over a period of 21 d. compared to ...199412232273
characterization of blue-green fluorescence in the mesophyll of sugar beet (beta vulgaris l.) leaves affected by iron deficiency.the mesophyll of sugar beet (beta vulgaris l.) leaves emits red (chlorophyll a) fluorescence and blue-green fluorescence when excited with ultraviolet light. the intensity of blue-green fluorescence was increased in mesophylls affected by iron deficiency. this increase was large and progressive. it was concomitant with a decrease of photosynthetic pigments per unit of leaf area. most of the increase in blue-green fluorescence can be explained by the decrease of the screening of ultraviolet light ...199412232310
kinetics analysis of the plasma membrane sucrose-h+ symporter from sugar beet (beta vulgaris l.) leaves.the kinetics behavior of the h+-sucrose (suc) symporter was investigated in plasma membrane vesicles from sugar beet (beta vulgaris l.) leaves by analyzing the effect of external and internal ph (pho and phi, respectively) on suc uptake. the apparent km for suc uptake increased 18-fold as the pho increased from 5.5 to 7.5. over this same pho range, the apparent vmax for suc uptake remained constant. the effects of phi in the presence or absence of internal suc were exclusively restricted to chan ...199412232379
comparison of the stereospecificity and immunoreactivity of nadh-ferricyanide reductases in plant membranes.the substrate stereospecificity of nadh-ferricyanide reductase activities in the inner mitochondrial membrane and peroxisomal membrane of potato (solanum tuberosum l.) tubers, spinach (spinacea oleracea l.) leaf plasma membrane, and red beetroot (beta vulgaris l.) tonoplast were all specific for the [beta]-hydrogen of nadh, whereas the reductases in wheat root (triticum aestivum l.) endoplasmic reticulum and potato tuber outer mitochondrial membrane were both [alpha]-hydrogen specific. in all is ...199412232391
modifications of etioplasts in cotyledons during prolonged dark growth of sugar beet seedlings (identification of etiolation-related plastidial aminopeptidase activities).we studied the effects of prolonged dark growth on proplastids and etioplasts in cotyledons of sugar beet (beta vulgaris l.) seedlings. differentiation of proplastids into etioplasts occurred between d 4 and d 6 after imbibition, with the typical characteristics of increased synthesis of plastidial proteins, protein and carotenoid accumulation, size increase, development of plastid membranes and of the prolamellar body, and increase of the greening capacity. however, this situation of efficient ...199412232431
biosynthesis and radical scavenging activity of betalains during the cultivation of red beet (beta vulgaris) hairy root cultures.betalains biosynthesis and antiradical scavenging activity were investigated during cultivation of four hairy root cultures of beta vulgaris, obtained from different cultivars (bordo, egyptian, detroit 2 and detroit dark red). the best producer of betalains was a hairy root culture from beta vulgaris cv. detroit dark red (13.27 mg/g dry weight total pigment production). the ethanol extract, derived from roots of the same culture grown for 15 days under submerged conditions, showed a high antirad ...200912240990
calcium-activated k+ channels and calcium-induced calcium release by slow vacuolar ion channels in guard cell vacuoles implicated in the control of stomatal closure.stomatal closing requires the efflux of k+ from the large vacuolar organelle into the cytosol and across the plasma membrane of guard cells. more than 90% of the k+ released from guard cells during stomatal closure originates from the guard cell vacuole. however, the corresponding molecular mechanisms for the release of k+ from guard cell vacuoles have remained unknown. rises in the cytoplasmic ca2+ concentration have been shown to trigger ion efflux from guard cells, resulting in stomatal closu ...199412244253
identification and characterization of high-affinity binding sites for inositol trisphosphate in red beet.inositol 1,4,5-trisphosphate (insp3) is thought to play a primary role in intracellular ca2+ mobilization during signal transduction in plant cells. although insp3-elicited ca2+ release across the vacuolar membrane has been demonstrated in a variety of species, little is known of the properties of the putative insp3 receptor. using a 3h-insp3 ligand-displacement assay with detergent-solubilized microsomes from the storage root of red beet, we determined that insp3 binds specifically to a single ...199312271091
supplemental betaine and peroxide-treated feather meal for finishing cattle.these studies evaluated the effects of betaine, provided either as feed-grade betaine or as concentrated separator by-product (csb; desugared beet molasses), on performance and carcass characteristics of finishing cattle. in exp. 1, 175 steers (410 kg initial bw) were fed a finishing diet based on steam-flaked and dry-rolled corn, and treatments included 10.5 and 21 g/d feed-grade betaine and 250 and 500 g/d csb (supplying 15.5 and 31 g/d of betaine, respectively). steers fed feed-grade betaine ...200212349999
the provision of drinking water to veal calves for welfare purposes.growth performance, behavior, physiology, forestomach development, abomasal lesions, and meat quality of veal calves fed a milk-replacer diet (no water) were compared to those obtained from calves fed the same diet and provided with increasing amounts of drinking water (water). two groups of 69 polish friesian calves, balanced according to initial bw, were assigned to two water treatments in a 3 x 2 x 2 factorial arrangement that provided solid feed in addition to the milk-replacer diet (no soli ...200212350013
identification and characterization of a nacl-inducible vacuolar na+/h+ antiporter in beta vulgaris.we have cloned, by rt-pcr and the use of degenerate oligonucleotide primers, a na+/h+ antiporter from beta vulgaris that is homologous to nhx1 of arabidopsis thaliana and is a member of the family of recently cloned plant nhx-genes. this antiporter, bvnhx1, partially complements the salt-sensitive phenotype of a deltaena1-4deltanhx1 yeast strain. antibodies were raised against a central portion of the bvnhx1 open reading frame that was predicted from the cloned cdna. this antiporter was found to ...200212354197
subcellular localization and isoenzyme pattern of peroxidase and polyphenol oxidase in beet root (beta vulgaris l.).the two enzymes involved in enzymatic browning reactions, polyphenol oxidase (ppo) and peroxidase (po), have been partially purified and extracted from different fractions of beet root. ppo is mainly located in the membrane fraction, and it was also found in the soluble fraction. in both cases ppo was in its latent state. however, po activity was higher in the soluble fraction than in the membrane fraction. nevertheless, the highest values of specific activity for po were obtained from the solub ...200212358490
phyllobacterium myrsinacearum (subjective synonym phyllobacterium rubiacearum) emend.the taxonomic relationships between phyllobacterium myrsinacearum lmg 2t2t, phyllobacterium rubiacearum lmg 1t1t and two phyllobacterium strains representing a collection of isolates from sugar-beet roots were investigated by means of fatty acid analysis and dna-dna hybridization. the strains showed very similar fatty acid compositions and more than 96% dna reassociation to each other. on the basis of these results and available data from the literature, it is proposed that p. rubiacearum be cla ...200212361292
interaction between long-distance transport factor and hsp70-related movement protein of beet yellows virus.systemic spread of viruses in plants involves local movement from cell to cell and long-distance transport through the vascular system. the cell-to-cell movement of the beet yellows virus (byv) is mediated by a movement protein that is an hsp70 homolog (hsp70h). this protein is required for the assembly of movement-competent virions that incorporate hsp70h. by using the yeast two-hybrid system, in vitro coimmunoprecipitation, and in planta coexpression approaches, we show here that the hsp70h in ...200212368343
fecal inoculum can be used to determine the rate and extent of in vitro fermentation of dietary fiber sources across three lemur species that differ in dietary profile: varecia variegata, eulemur fulvus and hapalemur griseus.to estimate fermentative capacity among lemur species, four fiber substrates were tested across three species, eulemur fulvus, hapalemur griseus and varecia variegata. the substrates, cellulose, beet pulp, citrus pulp and citrus pectin, ranged in composition from completely insoluble fiber (if) to completely soluble fiber (sf), respectively. the lemurs consumed a nutritionally complete biscuit formulated for primates [85 g/100 g diet dry matter (dm)] and locally available produce (15 g/100 g die ...200212368398
the beta-1,4-endogalactanase a gene from aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions.the aspergillus nigerbeta-1,4-endogalactanase encoding gene (gala) was cloned and characterized. the expression of gala in a. niger was only detected in the presence of sugar beet pectin, d-galacturonic acid and l-arabinose, suggesting that gala is coregulated with both the pectinolytic genes as well as the arabinanolytic genes. the corresponding enzyme, endogalactanase a (gala), contains both active site residues identified previously for the pseudomonas fluorescensbeta-1,4-endogalactanase. the ...200212383257
expression profiling of pectinolytic genes from aspergillus niger.the expression of 26 pectinolytic genes from aspergillus niger was studied in a wild type strain and a crea derepressed strain, under 16 different growth conditions, to obtain an expression profile for each gene. these expression profiles were then submitted to cluster analysis to identify subsets of genes with similar expression profiles. with the exception of the feruloyl esterase encoding genes, all genes were expressed in the presence of d-galacturonic acid, polygalacturonate, and/or sugar b ...200212387863
measuring agricultural sustainability in terms of efficiency: the case of dutch sugar beet growers.sustainability embraces socio-economic and bio-ecological dimensions or attributes. this paper presents a conceptual framework for quantifying sustainability on the basis of efficiency theory commonly used in economics. the conceptual model is implemented using data envelopment analysis (dea). sustainability is measured for a sample of dutch sugar beet growers. the average technical efficiency was only 50%. a positive correlation was found between technical efficiency and sustainable efficiency. ...200212395583
evaluation of atriplex lines for selenium accumulation, salt tolerance and suitability for a key agricultural insect pest.thirty atriplex lines were examined for potential habitat improvement and phytoremediation of selenium (se) contaminated sites. studies were conducted to determine the biomass production, se accumulation, and resistance of each line to the beet armyworm, spodoptera exigua, an agriculturally important insect. plants were tested using three salinity treatments: (1) control, no se; (2) nacl and cacl2 salts and 1 mg l(-1) se (12.7 microm) added as sodium selenate; and (3) iso-osmotic to treatment 2 ...200212395860
effect of molassed sugar beet pulp on nutrient utilisation and metabolic parameters during exercise.the aim of this study was to investigate the effect of partly replacing oats with molassed sugar beet pulp in a traditional hay/oat diet on nutrient utilisation and metabolic parameters in exercising horses. in a change-over experiment, 4 standardbred geldings were fed a hay and oat-based diet (oat diet) and a hay and oat-based diet where oats was partially replaced with molassed sugar beet pulp (msbp diet). each experimental period was 21 days during which total collection of faeces and urine w ...200212405658
phase ii enzyme-inducing and antioxidant activities of beetroot (beta vulgaris l.) extracts from phenotypes of different pigmentation.free-radical scavenging, reducing, and phase ii enzyme-inducing activities of aqueous and 5% aqueous ethanol extracts of freeze-dried root tissue of four beet (beta vulgaris l.) strains (red, white, orange, and high-pigment (red) phenotypes) were determined. aqueous and ethanolic tissue extracts of the regular and high-pigment red phenotypes were most capable of inhibiting metmyoglobin/h(2)o(2)-mediated oxidation of 2-2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (abts) and 2,2'-azobis-(2- ...200212405765
plant-cell bioreactors with simultaneous electropermeabilization and electrophoresis.experimental investigations on using low-level electric currents and voltages to extract, transport, and collect intracellular secondary metabolites from plant cells while maintaining their viabilities were conducted focusing on the production of: (1) ionic betalains, mainly negatively-charged betanin, from beta vulgaris cells, and (2) ionic alkaloids, particularly positively-charged ajmalicine and yohimbine, from catharanthus roseus cells. three versions of tubular membrane reactors in which el ...200312413782
characterisation of pectin subunits released by an optimised combination of enzymes.pectins from sugar beet, lime and apple were degraded by a rhamnogalacturonan hydrolase associated or not with pectin methylesterases and side chain degrading enzymes (galactanase and arabinanase). the composition of the enzymatic mixture was optimised by following the reaction by viscosimetric means. the reaction products were fractionated by ion exchange chromatography. treatment with all the enzymes released four fractions: (1). 227-247 mg/g of initial pectins and corresponded to neutral suga ...200212423972
the beet soilborne pomovirus in belgium and relationship with rhizomania.the objective of this study was to determine the extend of the beet soilborne pomovirus (bsbv) and the beet virus q in sugar beet fields in belgium. during the 2000 sugar beet growing season, more than 80 fields located in belgium were investigated for the presence of the beet necrotic yellow vein benyvirus (bnyvv), the bsbv and polymyxa betae, the plasmodiophorid vector of both viruses. fields investigated were identified either using previous recorded data or by visual identification of yellow ...200112425019
analysis of nematode-responsive promoters in sugar beet hairy roots.one of the strategies to make crops resistant to the beet cyst nematode heterodera schachtii is the destruction of the feeding site or syncytium. this can be achieved by local expression of the cytotoxic barnase gene under control of a nematode-inducible plant promoter that is active in the syncytium. expression of barnase outside the feeding site has to be neutralized by its inhibitor barstar driven from a constitutive promoter that is downregulated in the syncytium. several promoters that are ...200112425082
alternate energy-dependent pathways for the vacuolar uptake of glucose and glutathione conjugates.through the development and application of a liquid chromatography-mass spectrometry-based procedure for measuring the transport of complex organic molecules by vacuolar membrane vesicles in vitro, it is shown that the mechanism of uptake of sulfonylurea herbicides is determined by the ligand, glucose, or glutathione, to which the herbicide is conjugated. atp-dependent accumulation of glucosylated chlorsulfuron by vacuolar membrane vesicles purified from red beet (beta vulgaris) storage root app ...200212428021
a non-modular type b feruloyl esterase from neurospora crassa exhibits concentration-dependent substrate inhibition.feruloyl esterases, a subclass of the carboxylic acid esterases (ec 3.1.1.1), are able to hydrolyse the ester bond between the hydroxycinnamic acids and sugars present in the plant cell wall. the enzymes have been classified as type a or type b, based on their substrate specificity for aromatic moieties. we show that neurospora crassa has the ability to produce multiple ferulic acid esterase activities depending upon the length of fermentation with either sugar beet pulp or wheat bran substrates ...200312435269
protection by beverages, fruits, vegetables, herbs, and flavonoids against genotoxicity of 2-acetylaminofluorene and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) in metabolically competent v79 cells.chinese hamster lung fibroblasts, genetically engineered for the expression of rat cytochrome p450 dependent monooxygenase 1a2 and rat sulfotransferase 1c1 (v79-rcyp1a2-rsult1c1 cells), were utilized to check for possible protective effects of beverages of plant origin, fruits, vegetables, and spices against genotoxicity induced by 2-acetylaminofluorene (aaf) or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip). antigenotoxic activities of juices from spinach and red beets against aaf could ...200212438004
the effects of chard (beta vulgaris l. var. cicla) extract on the kidney tissue, serum urea and creatinine levels of diabetic rats.the aim of this work was to investigate the effects of chard (beta vulgaris l. var. cicla) extract on serum urea and creatinine concentrations and on kidney tissue in normal and streptozotocin-diabetic rats. the extract was administered to rats at a dose of 2 g/kg every day for 28 days, 14 days after animals were made diabetic. on day 42, kidney tissue and blood samples were examined. significant degenerative changes in kidney tissue of diabetic rats were observed, but in the group given chard e ...200212458482
mapping the probability of exceeding critical thresholds for cadmium concentrations in soils in the netherlands.the probability of exceeding critical thresholds of cd concentrations in the soil was mapped at a national scale. the critical thresholds in soil were based on food quality criteria for cd in crops or in organs of cattle (bos taurus), and were calculated by inverting a regression model for the cd concentration in the crop, with the cd concentration in soil, soil organic matter (som) content, clay content, and ph as predictors. the probability of exceeding the critical threshold for cd in soil pe ...200212469837
the complete sequence of the rice (oryza sativa l.) mitochondrial genome: frequent dna sequence acquisition and loss during the evolution of flowering plants.the entire mitochondrial genome of rice (oryza sativa l.), a monocot plant, has been sequenced. it was found to comprise 490,520 bp, with an average g+c content of 43.8%. three rrna genes, 17 trna genes and five pseudo trna sequences were identified. in addition, eleven ribosomal protein genes and two pseudo ribosomal protein genes were found, which are homologous to 13 of the 16 genes for ribosomal proteins in the mitochondrial genome of the liverwort (marchantia polymorpha). a greater degree o ...200212471441
construction of a 'unigene' cdna clone set by oligonucleotide fingerprinting allows access to 25 000 potential sugar beet genes.access to the complete gene inventory of an organism is crucial to understanding physiological processes like development, differentiation, pathogenesis, or adaptation to the environment. transcripts from many active genes are present at low copy numbers. therefore, procedures that rely on random est sequencing or on normalisation and subtraction methods have to produce massively redundant data to get access to low-abundance genes. here, we present an improved oligonucleotide fingerprinting (ofp ...200212472698
swiss chard: a salad crop for the space program.salad greens will be among the first crops grown on lunar or planetary space stations. swiss chard (beta vulgaris l.) is an important candidate salad crop because it is high yielding and rich in vitamins and minerals. five swiss chard cultivars were grown in the greenhouse under two light levels for 13 weeks to compare cumulative yields from weekly harvests, mineral composition, and to evaluate sensory attributes as a salad green. the varieties large white ribbed (lwr) and lucullus (luc) were th ...200212481809
modulation of cd8+ intraepithelial lymphocyte distribution by dietary fiber in the rat large intestine.we studied whether ingestion of dietary fiber modifies the distribution of intraepithelial lymphocytes (iel) in a physiological condition. male wkah rats were fed diets either with fiber (sugar beet fiber or crystalline cellulose, 100 g/kg diet each) or without fiber for 3 weeks. the number of cd8(+), cd4(+), and nkr-p1(+) iel per epithelial layer in the crypt section of the cecum, proximal colon, and distal colon were scored by immunohistochemical staining. we found that the proportion of cd8(+ ...200212486212
the complete nucleotide sequence of beet black scorch virus (bbsv), a new member of the genus necrovirus.the complete nucleotide sequence of beet black scorch virus (bbsv) was determined. the bbsv genome is composed of 3641 nucleotides and has similar organization with tobacco necrosis virus d of 61% nucleotide identity. the 5'-proximal open reading frame (orf) encodes a putative 23 kda protein and a 82 kda protein by reading-through of an amber termination codon. three small orfs located in the center of the genome may encode for a 4.2 kda protein and two 7 kda proteins. the 3'-proximal orf encode ...200212491108
the mobile bag technique as a method for determining the degradation of four botanically diverse fibrous feedstuffs in the small intestine and total digestive tract of ponies.an in sacco mobile bag technique was used to determine the rate and extent of disappearance of unmolassed sugar-beet pulp, soyabean hulls, hay cubes and an oat hull-naked oats mixture (67:33, w/w) in the foregut and total digestive tract of ponies. ponies were administered naso-gastrically polyester mesh bags containing 350 mg feed, in a latin square design. bags were collected at the ileo-caecal junction (small intestine bag, sib) and in the faeces (faecal bag, fb) and their residues analysed f ...200212493095
effects of chard (beta vulgaris l. var. cicla) extract on oxidative injury in the aorta and heart of streptozotocin-diabetic rats.in diabetes mellitus, increased free radical formation raises the incidence of atherosclerosis and cardiovascular diseases. regardless of the type of diabetes, the objective of the therapy is to achieve normoglycemia and to prevent or delay the complications. chard (beta vulgaris l. var. cicla) is used as a hypoglycemic agent by diabetic patients in turkey. the aim of this study was to investigate the effect of feeding chard on diabetes-induced free radical-mediated injury in rat aorta and heart ...200212511111
a host-specific bacteria-to-plant signal molecule (nod factor) enhances germination and early growth of diverse crop plants.lipo-chitooligosaccharides (lcos), or nod factors, are host-specific bacteria-to-plant signal molecules essential for the establishment of a successful n(2)-fixing legume-rhizobia symbiosis. at submicromolar concentrations nod factors induce physiological changes in host and non-host plants. here we show that the nod factor nod bj v(c18:1,mefuc) of bradyrhizobium japonicum 532c enhances germination of a variety of economically important plants belonging to diverse botanical families: zea mays, o ...200312520335
the companion cell-specific arabidopsis disaccharide carrier atsuc2 is expressed in nematode-induced syncytia.cyst nematodes induce a metabolically highly active syncytial cell complex in host roots. the syncytia are symplastically isolated. because they form a strong sink, assimilates must be imported via the apoplast, thus suggesting that specific membrane-bound sugar transport proteins are expressed and activated. to identify possible candidate genes, transgenic arabidopsis plants expressing different reporter genes under the control of different promoters from arabidopsis sugar transporter genes wer ...200312529515
adaptation to the digestion of nutrients of a starch diet or a non-starch polysaccharide diet in group-housed pregnant sows.a trial was conducted with twenty group-housed pregnant sows to study the adaptation in nutrient digestibility to a starch-rich diet or a diet with a high level of fermentable non-starch polysaccharides (nsp) during a time period of 6 weeks. the starch-rich diet was primarily composed of wheat, peas and tapioca, whereas soya bean hulls and sugar beet pulp, which both are highly fermentable nsp sources, were used to formulate the nsp-rich diet. the starch-rich diet and the nsp-rich diet were form ...200212534834
the ability of betaine to reduce the irritating effects of detergents assessed visually, histologically and by bioengineering methods.a novel approach for reducing the undesired irritating properties of detergents on skin might be offered by betaine, which is a natural product derived from the sugar beet. the aim of the study was to explore the ability of betaine to reduce the irritating effects of two surfactants, sodium lauryl sulphate (sls) and cocoamidopropylbetaine (capb). for evaluation of changes in skin reactions visual scoring, electrical impedance, transepidermal water loss and histology were used.200312535285
expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (beta vulgaris l.).three genes from ralstonia eutropha necessary for poly(3-hydroxybutyrate) (phb) synthesis were introduced into the hairy roots of sugar beet. transformation of a vector construct harbouring the phb genes, each fused to the coding region of the pea ribulose-bisphosphate carboxylase plastid targeting sequence, resulted in 20 transgenic hairy-root clones, producing up to 55 mg high molecular phb/g dry weight, as identified by gas chromatography, gel permeation chromatography and hplc. accumulation ...200312536258
effects of dietary protein and fermentable fiber on nitrogen excretion patterns and plasma urea in grower pigs.effects of dietary protein concentration (high, 18.5; low, 15.7%) and fermentable fiber (control; soyhulls, sh; and sugar beet pulp, sbp) on n excretion patterns and plasma urea were tested in a 2 x 3 factorial arrangement. the objectives were: 1) to determine if reduced dietary protein together with fermentable fiber would reduce urinary n excretion further than a single diet manipulation, 2) to determine if effects of diet manipulations were similar between pigs with restricted and free access ...200212542166
chemoprevention of dmba-induced uv-b promoted, nor-1-induced tpa promoted skin carcinogenesis, and den-induced phenobarbital promoted liver tumors in mice by extract of beetroot.our previous studies identified the extract of beta vulgaris (beetroot), commercially also known as betanin, as a potent cancer chemopreventive agent in both in vitro epstein-barr early antigen activation assay and in an in vivo two-stage mouse lung and skin carcinogenesis. to explore this issue further, we have now investigated its cancer chemopreventive potentials in three different chemical carcinogen initiation-promotion experimental tumor models in mice. following tumor initiation with 390 ...200312543062
regulation of the fast vacuolar channel by cytosolic and vacuolar potassium.at resting cytosolic ca(2)(+), passive k(+) conductance of a higher plant tonoplast is likely dominated by fast vacuolar (fv) channels. this patch-clamp study describes k(+)-sensing behavior of fv channels in beta vulgaris taproot vacuoles. variation of k(+) between 10 and 400 mm had little effect on the fv channel conductance, but a pronounced one on the open probability. shift of the voltage dependence by cytosolic k(+) could be explained by screening of the negative surface charge with a dens ...200312547779
the 64-kilodalton capsid protein homolog of beet yellows virus is required for assembly of virion tails.the filamentous virion of the closterovirus beet yellows virus (byv) consists of a long body formed by the major capsid protein (cp) and a short tail composed of the minor capsid protein (cpm) and the virus-encoded hsp70 homolog. by using nano-liquid chromatography-tandem mass spectrometry and biochemical analyses, we show here that the byv 64-kda protein (p64) is the fourth integral component of byv virions. the n-terminal domain of p64 is exposed at the virion surface and is accessible to anti ...012551975
potassium-selective channel in the red beet vacuolar membrane.in higher plants the vacuolar k(+)-selective (vk) channel was identified solely in guard cells. this patch-clamp study describes a 40 ps homologue of the vk channel in beta vulgaris taproot vacuoles. this voltage-independent channel is activated by submicromolar ca(2+), and is ideally selective for k(+) over cl(-) and na(+).200312554709
feed intake of young double-muscled bulls fed on grass and supplemented with sugar-beet pulp.daily dry matter intake in young growing double-muscled bulls, fed indoors on grass, was estimated based on forty-four intake data from 28 animals, ageing at least five months and weighing up to 400 kg live weight. intake was measured during five consecutive days using one of eighteen cuts of grass. fresh meadow grass (mainly lolium perenne) was fed ad libitum and two kg dried sugar-beet pulp was offered per animal and per day. animal live weight averaged 278 +/- 82 kg and mean total daily dry m ...200212556046
effect of diet and gnrh administration on post-partum ovarian cyclicity in autumn-lambing ewes.using autumn-lambing ewes, this study investigated (i) the effects of diet on gonadotrophin secretion and responsiveness of the hypothalamic-pituitary-ovarian axis to exogenous gnrh during the early post-partum period; and (ii) whether ovulation prior to completion of uterine involution results in an increased incidence of aberrant ovarian cycles. thirty-two ewes rearing 1.9+/-0.12 lambs were equally allocated to two dietary treatments at lambing (22 october +/-0.2 day). diets comprised ad libit ...200312559721
quantitative determination of geosmin in red beets (beta vulgaris l.) using headspace solid-phase microextraction.an improved analytical method for the determination of geosmin in red beets was developed using headspace solid-phase microextraction (hspme). volatiles of beet juice were extracted in headspace for 2 h using a polydimethylsiloxane/divinylbenzene fiber, thermally desorbed from the fiber, and analyzed by gas chromatography. the hspme method was determined to be suitable for geosmin analysis as evidenced by high relative recovery (99.2%), low relative standard deviation (7.48%), and reasonable det ...200312568566
biosynthetic origin of geosmin in red beets (beta vulgaris l.).geosmin provides the characteristic but sometimes undesirable "earthy" flavor to red table beets. to date, it is not known whether geosmin is a byproduct of beet metabolism or synthesized by soil-borne microorganisms and taken up by the beets during maturation. analysis of mature beet roots revealed that peels contained 6 times the amount of geosmin compared to the bodies and cores. sterilized beet seeds were aseptically grown in a basal medium prior to analysis for the presence of geosmin. usin ...200312568567
quantitative relationships between induced jasmonic acid levels and volatile emission in zea mays during spodoptera exigua herbivory.jasmonic acid (ja) has long been hypothesized to be an important regulator of insect-induced volatile emission; however, current models are based primarily on circumstantial evidence derived from pharmacological studies. using beet armyworm caterpillars (baw: spodoptera exigua) and intact corn seedlings, we examine this hypothesis by measuring both the time-course of insect-induced ja levels and the relationships between endogenous ja levels, ethylene, indole and sesquiterpenes. in separate morn ...200312569409
production of cyclic lipopeptides by pseudomonas fluorescens strains in bulk soil and in the sugar beet rhizosphere.the production of cyclic lipopeptides (clps) with antifungal and biosurfactant properties by pseudomonas fluorescens strains was investigated in bulk soil and in the sugar beet rhizosphere. purified clps (viscosinamide, tensin, and amphisin) were first shown to remain highly stable and extractable (90%) when applied (ca. 5 microg g(-1)) to sterile soil, whereas all three compounds were degraded over 1 to 3 weeks in nonsterile soil. when a whole-cell inoculum of p. fluorescens strain dr54 contain ...200312571005
surface motility in pseudomonas sp. dss73 is required for efficient biological containment of the root-pathogenic microfungi rhizoctonia solani and pythium ultimum.pseudomonas sp. dss73 was isolated from the rhizoplane of sugar beet seedlings. this strain exhibits antagonism towards the root-pathogenic microfungi pythium ultimum and rhizoctonia solani. production of the cyclic lipopeptide amphisin in combination with expression of flagella enables the growing bacterial culture to move readily over the surface of laboratory media. amphisin is a new member of a group of dual-functioning compounds such as tensin, viscosin and viscosinamid that display both bi ...200312576578
the use of rapd analysis of sugar-beet mitochondrial dna in search of cms genes. 201612577632
using seed purity data to estimate an average pollen mediated gene flow from crops to wild relatives.gene flow from crops to wild related species has been recently under focus in risk-assessment studies of the ecological consequences of growing transgenic crops. however, experimental studies addressing this question are usually temporally or spatially limited. indirect population-structure approaches can provide more global estimates of gene flow, but their assumptions appear inappropriate in an agricultural context. in an attempt to help the committees providing advice on the release of transg ...200212579439
high-resolution mapping of the bolting gene b of sugar beet.sugar beet ( beta vulgaris l.) is a biennial species. shoot elongation (bolting) starts after a period of low temperature. the dominant allele of locus b causes early bolting without cold treatment. this allele is abundant in wild beets whereas cultivated beets carry the recessive allele. fifteen aflp markers, tightly linked to the bolting locus, have been identified using bulked segregant analysis. the f(2)-population consisted of 2,134 individuals derived after selfing a single f(1)-plant ( bb ...200212582567
mapping qtls for sucrose content, yield and quality in a sugar beet population fingerprinted by est-related markers.seventy five expressed sequence tags (ests) that are associated with functions in carbohydrate and nitrogen metabolism were genotyped in 108 plants of an f2 population of sugar beet ( beta vulgaris l.) segregating for sugar quality and yield parameters. supplemented by known rflp and aflp markers, the resulting map spans 446 cm of the 758-mbp genome of sugar beet. f3 test-cross plants were analysed for corrected sugar yield, beet yield, ion balance and the content of sugar, amino nitrogen, potas ...200212582619
crop-weed interactions in the beta vulgaris complex at a local scale: allelic diversity and gene flow within sugar beet fields.crop-wild hybrids and weed beets are the main source of agronomic concern for sugar beet production all over europe. in order to understand the dynamics of crop-wild interactions and the evolution of weediness in beta vulgaris, we investigated genetic features of bolting individuals occurring at a local scale, i.e. within two sugar beet fields of the french northern area of sugar beet production. by analysing ploidy level, mitochondrial dna and microsatellite polymorphism, the genetic diversity ...200212582675
the sugar beet mitochondrial nad4 gene: an intron loss and its phylogenetic implication in the caryophyllales.the sugar beet mitochondrial gene for subunit iv of nadh dehydrogenase ( nad4) has been characterized. unlike the corresponding genes in wheat and turnip, sugar beet nad4 lacks the second intron ( nad4-i2). northern-blot analysis demonstrates transcription of the gene. a total of 19 rna editing sites were identified in the sugar beet nad4 transcripts; interestingly, there is no editing in the region which flanks the lost intron. this observation is in favour of intron loss via homologous recombi ...200212582688
leader proteinase of beet yellows virus functions in long-distance transport.the 66-kda leader proteinase (l-pro) of the beet yellows virus (byv) possesses a nonconserved n-terminal domain and a conserved, papain-like c-terminal domain. previous work revealed that the n-terminal domain functions in rna amplification, whereas the c-terminal domain is required for autoproteolysis. alanine-scanning mutagenesis was applied to complete the functional analysis of l-pro throughout the virus life cycle. this analysis indicated that the c-terminal domain of l-pro, in addition to ...200312584307
multitasking in replication is common among geminiviruses.geminiviruses package single-stranded circular dna and replicate via double-stranded dna intermediates. during the past decade, increasing evidence has led to the general acceptance that their replication follows a rolling-circle replication mechanism like bacteriophages with single-stranded dna. in a recent study, we showed that this is also true for abutilon mosaic geminivirus (abmv), but that this particular virus may also use a recombination-dependent replication (rdr) route in analogy to t4 ...200312584322
effects of point mutations in the major capsid protein of beet western yellows virus on capsid formation, virus accumulation, and aphid transmission.point mutations were introduced into the major capsid protein (p3) of cloned infectious cdna of the polerovirus beet western yellows virus (bwyv) by manipulation of cloned infectious cdna. seven mutations targeted sites on the s domain predicted to lie on the capsid surface. an eighth mutation eliminated two arginine residues in the r domain, which is thought to extend into the capsid interior. the effects of the mutations on virus capsid formation, virus accumulation in protoplasts and plants, ...200312584348
identification and exploitation of novel disease resistance genes in sugar beet.sugar beet is a valuable arable crop providing a large proportion of world sugar supplies. it is susceptible to a range of fungal and viral diseases, to most of which there is inadequate genetic resistance. control instead relies largely on the use of pesticides. to increase the sustainability of sugar-beet cultivation, strong and durable genetic resistance is urgently needed. this review describes an iacr-broom's barn programme devoted to evaluating beta germplasm for novel resistance to major ...200312587876
variability of cathodic peroxidases in sugar beet (beta vulgaris l.) cultivars.eighteen varieties of sugar beet (beta vulgaris l.), originating from various european countries, were compared in respect of peroxidase variability level. they were cultivated in the same experimental plot. the cultivars differed in ploidy level: one variety was tetraploid, three were diploid and 14 varieties were triploid. the cathodic peroxidase system is controlled by four independent genes, of which only one is polymorphic. consequently, the varieties were characterised by frequencies of 3 ...200312590181
effects of dietary fermentable carbohydrates on behavior and heat production in group-housed sows.the effects of dietary nonstarch polysaccharides (nsp) on behavior and heat production in group-housed sows were studied. twelve groups of six nonpregnant sows were fed one of four experimental diets that were similar in composition except for starch and nsp contents. exchanging sugar beet pulp silage (sbps) for tapioca created the difference in dietary starch and nsp ratio. on a dry matter (dm) basis, diets contained 0, 10, 20, or 30% sbps. sows were group-housed. intake of fermentable nsp (fns ...200312597389
[the influence of vegetable biologically active food additives on men's health].the young people of draft age often have breach of a the dietary, that guite often is accompanied by decrease resistance and adaptation of opportunities. application vegetative bas to food (the beet with selderej) separately or especially together with liquid biphidiumbacterin gives good therapeutic effect.200212599995
arabidopsis gene expression changes during cyst nematode parasitism revealed by statistical analyses of microarray expression profiles.with the availability of microarray technology, the expression profiles of thousands of genes can be monitored simultaneously to help determine the mechanisms of these biological processes. we conducted affymetrix genechip microarray analyses of the arabidopsis-cyst nematode interaction and employed a statistical procedure to analyze the resultant data, which allowed us to identify significant gene expression changes. quantitative real-time rt-pcr assays were used to confirm the microarray analy ...200312609032
distillery effluent effect on seed germination, early seedling growth and pigment content of sugarbeet (beta vulgaris linn. var. mezzanau-poly).bioassay studies were carried out to assess the toxicity of distillery effluent on seed germination, seedling growth and pigment contents. higher concentrations (> 5%) of effluent were found to be toxic but however, can be used for irrigational purpose after proper dilution.200212617322
[topical pectins for the treatment of burn wounds].the results of the burned wounds treatment with 1-2% solution of apple or beet pectins were analysed. the control results were examined at the same patients with symmetrical wounds treated by chlorhexidine, levomecol, furacillin liniment. clinical evaluation demonstrated good tolerability of the treatment regime, absence of side effects and complications, inhibition of inflammation, acceleration of epithelisation of the burns ii-iiia class, allowed to diminish the preliminary period before autod ...200212619514
a novel approach to the use of genetically modified herbicide tolerant crops for environmental benefit.the proposed introduction of genetically modified herbicide tolerant (gmht) crops, with claims of improved weed control, has prompted fears about possible environmental impacts of their widespread adoption, particularly on arable weeds, insects and associated farmland birds. in response to this, we have developed a novel weed-management system for gmht sugar beet, based on band spraying, which exploits the flexibility offered by the broad-spectrum partner herbicides. here, we show the results fr ...200312639311
suppressor of rna silencing encoded by beet yellows virus.using an agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the beet yellows virus for proteins with rna silencing suppressor activity. among eight proteins tested, only a 21-kda protein (p21) was able to suppress double-stranded (ds) rna-induced silencing of the green fluorescent protein (gfp) mrna. restoration of gfp expression by p21 under these conditions had no apparent effect on accumulation of the small interfering rnas. in addition, p21 elevated the transient expre ...200312642093
providence virus: a new member of the tetraviridae that infects cultured insect cells.we identified a new member of the tetraviridae, providence virus (prv), persistently infecting a midgut cell line derived from the corn earworm (helicoverpa zea). virus purified from these cells also productively infected a h. zea fat body cell line, and a cell line from whole embryos of the beet armyworm, spodoptera exigua. prv is thus the first tetravirus shown to replicate in cell culture. prv virions are isometric particles composed of two structural proteins (60 and 7.4 kda) that encapsidat ...200312642108
effects of adding fiber sources to reduced-crude protein, amino acid-supplemented diets on nitrogen excretion, growth performance, and carcass traits of finishing pigs.two experiments were conducted to evaluate the effects of adding fiber sources to reduced-crude protein (cp), amino acid-supplemented diets on n excretion, growth performance, and carcass traits of growing-finishing pigs. in exp. 1, six sets of four littermate barrows (initial weight = 36.3 kg) were allotted randomly to four dietary treatments to determine n balance and slurry composition. dietary treatments were: 1) fortified corn-soybean meal, control, 2) as fortified corn-soybean meal with cp ...200312643494
detection of sugar syrups in apple juice by delta(2)h per thousand and delta(13)c per thousand analysis of hexamethylenetetramine prepared from fructose.an improved procedure for determining (13)c and (2)h isotope ratios, using gas chromatography-isotope ratio mass spectrometry (gc-irms), has been developed for identifying the addition of low cost commercial sugar syrups to apple juices and related products. isotopic techniques are commonly used to identify the addition of low cost sugars to fruit juices and are difficult to circumvent as it is not economically viable to change the isotopic ratios of the sugars. the procedure utilizes the deriva ...200312643633
synergistic interactions between volicitin, jasmonic acid and ethylene mediate insect-induced volatile emission in zea mays.plants display differential responses following mechanical damage and insect herbivory. both caterpillar attack and the application of caterpillar oral secretions (os) to wounded leaves stimulates volatile emission above mechanical damage alone. volicitin (n-17-hydroxylinolenoyl-l-glutamine), present in beet armyworm (baw, spodoptera exigua) os, is a powerful elicitor of volatiles in excised maize seedlings (zea mays cv. delprim). we consider some of the mechanistic differences between wounding ...200312654041
antisense inhibition of mitochondrial pyruvate dehydrogenase e1alpha subunit in anther tapetum causes male sterility.we hypothesized that cytoplasmic male sterility (cms) in sugar beet may be the consequence of mitochondrial dysfunctions affecting normal anther development. to test the hypothesis, we attempted to mimic the sugar beet cms phenotype by inhibiting the expression of mitochondrial pyruvate dehydrogenase (pdh), which is essential for the operation of the tricarboxylic acid (tca) cycle. screening with a cdna library of sugar beet flower buds allowed the identification of two pdh e1alpha subunit genes ...200312662309
mechanisms of cr(iii) and cr(vi) removal from aqueous solutions by sugar beet pulp.the removal of three- and hexavalent chromium from aqueous solutions using sugar beet pulp as biosorbent substratewas performed. the kinetics of cr(iii) and cr(vi) removal were studied at 20.0 +/- 0.5 degrees c and under various experimental conditions. the cr(iii) ions were adsorbed onto the biosorbent by ion-exchange mechanism with ca2+ cations. the influence of solution ph was found to greatly affect the adsorption efficiency of cr(vi). cr(vi) removal was largely involved in a reduction mecha ...200312666794
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