Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| microvasculature of the buffalo (bubalus bubalis) choroid plexuses: structural, histochemical, and immunocytochemical study. | the choroid plexuses (cps) in mammals produce the cerebrospinal fluid (csf). in the literature, the morphology of cps and the process that regulates the production of csf are virtually nonexistent for domestic ruminants. thus this study has two aims: 1. to investigate the morpho-structure of the buffalo cp microvasculature utilizing light microscopy (lm), scanning electron microscopy (sem) and transmission electron microscopy (tem) techniques, and 2. to investigate the relationship between the b ... | 2011 | 21181712 |
| expression of pluripotency genes in buffalo (bubalus bubalis) amniotic fluid cells. | recent findings suggest that mammalian amniotic fluid (af) is a source of multipotent stem cells (scs), which can be used in regenerative medicine and assisted reproduction. we report the isolation, culture and characterization of amniotic fluid-derived cells from pregnant water buffalo uterus. these undifferentiated af cells expanded without feeder layer over a period of 100 days up to passages 20 and the expression of alkaline phosphatase (ap), oct-4, nanog and sox-2, gapdh and β-actin could b ... | 2010 | 21198969 |
| a global view of 54,001 single nucleotide polymorphisms (snps) on the illumina bovinesnp50 beadchip and their transferability to water buffalo. | the illumina bovinesnp50 beadchip features 54,001 informative single nucleotide polymorphisms (snps) that uniformly span the entire bovine genome. among them, 52,255 snps have locations assigned in the current genome assembly (btau_4.0), including 19,294 (37%) intragenic snps (i.e., located within genes) and 32,961 (63%) intergenic snps (i.e., located between genes). while the snps represented on the illumina bovine50k beadchip are evenly distributed along each bovine chromosome, there are over ... | 2010 | 21209788 |
| impact of pre-ovulatory follicle diameter on plasma estradiol, subsequent luteal profiles and conception rate in buffalo (bubalus bubalis). | the present study was designed to investigate the impact of pre-ovulatory follicle (pof) diameter on the day of estrus on plasma estradiol concentration, subsequent luteal profile (corpus luteum, cl, diameter and plasma progesterone concentration) and conception rate in buffaloes. twenty-eight buffaloes were synchronized with synthetic analogue of prostaglandin f(2α) (pgf(2α)) administered 11 days apart. transrectal ultrasonography and jugular vein blood sampling was carried out on the day of es ... | 2010 | 21215536 |
| green laser irradiation effects on buffalo semen. | the overall objective of this paper is to develop a more sensitive and less costly technique of laser irradiation of spermatozoa at certain wavelengths and exposure times suitable for improvement of buffalo semen quality. a 532 nm continuous wave (cw) dpss laser light has been used to irradiate buffalo semen for different time intervals. three semen pools from three different bulls (bubalus bubalis) were used in the experiment, each pool was divided into six groups : control (not irradiated), an ... | 2011 | 21220155 |
| chronological reorganization of microtubules, actin microfilaments, and chromatin during the first cell cycle in swamp buffalo (bubalus bubalis) embryos. | this paper aimed to study the dynamics of early embryonic development, in terms of redistribution of cytoskeleton (microtubules, actin microfilaments) and chromatin configurations during the first cell cycle in swamp buffalo embryos. oocytes were matured and fertilized in vitro, and they were fixed at various time points after ivf. at 6 h after ivf, 44.4% matured oocytes were penetrated by spermatozoa. partial zp digestion, however, did not improve fertilization rate compared to control (p > .05 ... | 2010 | 21234419 |
| sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings. | in this study, we compared the morphology of sarcocystis sinensis and sarcocystis hominis, and assessed the infectiousness of s. sinensis for human volunteers. the cysts of s. sinensis were from water buffalo (bubalus bubalis) and those of s. hominis were from cattle (bos taurus). transmission electron microscopy of s. sinensis cysts revealed that the cyst wall had leaning, finger-like protrusions measuring 1.44-5.08μm in length and without invaginations on the tip surface of the protrusions. in ... | 2010 | 21236581 |
| standardisation of an indirect enzyme-linked immunosorbent assay for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis) in italy. | an indirect enzyme-linked immunosorbent assay (elisa) was evaluated for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis linnaeus, 1758). the test accuracy was evaluated on milk samples from the campania region in italy. a total of 100 negative samples were collected from 10 officially brucellosis-free herds in salerno province, while 30 positive samples were collected from 3 herds in caserta province, where animals gave positive results to the official tests and ... | 2011 | 21240852 |
| effects of heavy metals and pesticides on buffalo (bubalus bubalis) spermatozoa functions in vitro. | industrial toxic metals, pollutants and bio-accumulative pesticides interfere with the male reproductive functions in farm animals. frozen-thawed semen samples were incubated with heavy metals (cadmium and lead) and pesticides (chlorpyrifos and endosulfan) of different concentrations (0, 0.005, 0.05, 0.02, 0.1, 0.5, 1.0, 2.0 and 4.0 μg/ml) for 1 h, and various spermatozoa functional parameters and in vitro fertilization rates were assessed. any significant effect was assessed by comparing the 1 ... | 2011 | 21241381 |
| free amino acids profile and quantities of 'sirt', 'bohca' and 'sekerpare' pastirma, dry cured meat products. | pastirma is a dry-cured and semi-fermented meat product, produced from whole beef or water buffalo muscles. sixteen to twenty different types of pastirma can be produced from a carcass, and each pastirma type has a different name, shape and quality characteristics. the objective of this study was to determine the free amino acid (faa) composition, ph and moisture values of 'sirt', 'bohca' and 'sekerpare' pastirma. | 2011 | 21254071 |
| ultrasonographic and endocrine aspects of follicle deviation, and acquisition of ovulatory capacity in buffalo (bubalus bubalis) heifers. | the objectives of this study were to determine the interval from ovulation to deviation and the diameter of the dominant (df) and largest subordinate (sf) follicles at deviation in buffalo (bubalus bubalis) heifers. two methods of evaluation (observed vs. calculated) were used. fsh and lh profiles encompassing follicle deviation (experiment 1), and the follicular diameter when the df acquired ovulatory capacity (experiment 2) were also determined. the time of deviation and the diameter of the df ... | 2010 | 21256685 |
| clinicopathological and ultrasonographic findings in 40 water buffaloes (bubalus bubalis) with traumatic pericarditis. | forty buffaloes with traumatic pericarditis were examined to characterise the ultrasonographic findings in buffaloes with traumatic pericarditis, determine the extent of the lesions and assess the prognosis. the most noticeable clinical presentations were presternal oedema (73 per cent) and jugular and mammary vein distension (88 per cent). laboratory findings included neutrophilic leucocytosis, elevated total protein concentration, hypoalbuminaemia, hypergammaglobulinaemia and increased concent ... | 2010 | 21262631 |
| analysis of active components of rhinoceros, water buffalo and yak horns using two-dimensional electrophoresis and ethnopharmacological evaluation. | cornu rhinoceri asiatici (rhinoceros horn, rh), cornu bubali (water buffalo horn, wbh), and cornu bovis grunniens (yak horn, yh) are traditional chinese medicine (tcm), and have been used in china for thousands of years. in this study, ethnopharmacological experiments were used to evaluate and verify the traditional efficacies of horns. area under curve (auc) was used to quantify the pharmacological efficacy strength of three horns. two-dimensional electrophoresis (2-de) was used to analyze the ... | 2011 | 21268260 |
| extended cytogenetic maps of sheep chromosome 1 and their cattle and river buffalo homoeologues: comparison with the oar1 rh map and human chromosomes 2, 3, 21 and 1q. | cytogenetic maps are useful tools for several applications, such as the physical anchoring of linkage and rh maps or genome sequence contigs to specific chromosome regions or the analysis of chromosome rearrangements. recently, a detailed rh map was reported in oar1. in the present study, we selected 38 markers equally distributed in this rh map for identification of ovine genomic dna clones within the ovine bac library chori-243 using the virtual sheep genome browser and performed fish mapping ... | 2011 | 21282943 |
| production of interspecies handmade cloned embryos by nuclear transfer of cattle, goat and rat fibroblasts to buffalo (bubalus bubalis) oocytes. | the possibility of producing interspecies handmade cloned (ihmc) embryos by nuclear transfer from donor cells of cattle, goat and rat using buffalo oocytes as recipient cytoplasts was explored. zona-free buffalo oocytes were enucleated by protrusion cone-guided bisection with a microblade. after electrofusion with somatic cells, reconstructed oocytes were activated by calcimycin a23187, treated with 6-dimethylaminopurine and were cultured in k-rvcl-50® medium for 8 days. although the cleavage ra ... | 2011 | 21288667 |
| long term effect of ovum pick-up in buffalo species. | the aim of this study was to evaluate the effect of an ovum pick-up (opu) treatment carried out for 9 months in buffalo (bubalus bubalis) species. eight pluriparous non-lactating buffalo cows underwent opu for 9 months. recovered cumulus enclosed oocytes (cocs) were classified and cocs suitable for in vitro embryo production (ivep) were in vitro matured (ivm), fertilized (ivf) and cultured (ivc) to the blastocyst (bl) stage. animals were monitored for a total period of 270 days, but at the summe ... | 2011 | 21306842 |
| dual promoter lentiviral vector generates transgenic mice expressing e2-csfv glycoprotein in their milk, but impairs early identification of transgenic embryos. | lentiviral vectors containing the green fluorescent protein gene have been successfully used to select transgenic embryos before transfer to a surrogate mother. however, there are apparently no reports regarding early detection of transgenic embryos using a lentiviral vector carrying an additional transcription unit for tissue-specific expression of a valuable protein. in this study, two hiv-based lentiviral vectors were constructed. the first one contained the green fluorescent protein (gfp) co ... | 2011 | 21316751 |
| assessment of buffalo (bubalus bubalis) sperm dna fragmentation using a sperm chromatin dispersion test. | chromosomal fragmentations or damage in sperm dna has considerable value in determination of semen quality. however, rapid and/or simple method to assess sperm dna integrity in buffalo has apparently not been reported. in the present study, scd was used for the first time in buffalo bulls for assessment of sperm dna fragmentation. a modified scd protocol, under bright field microscope was developed and validated by comparison with other routine tests which can be used for processing of samples. ... | 2011 | 21382103 |
| biochemical and hormonal composition of follicular cysts in water buffalo (bubalus bubalis). | the objective of this study was to examine the follicular fluid biochemical and hormonal changes associated with ovarian follicular cysts in buffalo. follicular fluid was aspirated from eight cysts and eight preovulatory follicles, and subjected to biochemical and hormonal analyses. cysts were characterized by a greater (p<0.01) concentration of nitric oxide and lesser concentrations of ascorbic acid and glucose than that of preovulatory follicles (p<0.01 and p<0.05, respectively). furthermore, ... | 2011 | 21392901 |
| assignment of genes to chromosome 4 of the river buffalo with a panel of buffalo-hamster hybrid cells. | summary: to identify the river buffalo chromosome carrying the genes coding for gapd, tpi1, and ldhb, karyotypic examination was carried out on 14 buffalo-hamster hybrid clones previously tested for presence of this syntenic group. in cattle, this group (u3) has been assigned to chromosome 5, which is assumed to be homologous to the long arm of buffalo chromosome 4. chromosome 4 was present in all five clones expressing the three enzymes, and absent in all seven negative clones, indicating that ... | 1993 | 21395717 |
| gene mapping in the river buffalo (bubalus bubalis l.): five syntenic groups. | summary: forty-five somatic buffalo-hamster hybrid clones were analysed for the presence or absence of pcr products of 10 primers. five syntenic groups were identified: cga-d9s1; cd18-d1s4; oxt-prnp; ldlr-d7s3; bspn-d14s2. these same syntenic groups were reported to be syntenic in cattle and were assigned to u2 (bta 9), u10 (bta 1), u11 (bta 13), u22 (bta 7), and u24 (bta 14), respectively. based on chromosomal homology between cattle and river buffalo chromosomes, these syntenic groups are expe ... | 1997 | 21395805 |
| use of molecular markers for the identification of river buffalo chromosomes: chromosome one. | a standard karyotype for the river buffalo has recently been established. the largest five chromosomes are biarmed and, based on the banding homology between cattle and buffalo chromosomes, were suggested to originate from the fusion of cattle acrocentric chromosomes. the origin of buffalo chromosome 1 is controversial due to the difficulty in differentiating between the small acrocentric cattle chromosomes. using molecular markers assigned to cattle chromosomes, synteny between cd18, a marker f ... | 1997 | 21395839 |
| a rapid improved method for sexing embryo of water buffalo. | the objective of the experiment of this paper is to develop and improve in the sexing method for preimplantation embryos of water buffalo (bubalus bubalis) using loop-mediated isothermal amplification (lamp) reaction. embryo sexing has been recognized to control effectively the sex of offspring in the embryo transfer industry. a rapid and simple detection system was established by adding ethidium bromide (eb) or 5 ++l of cuso4 (3m) to the product of lamp reaction. the result of these additions a ... | 2011 | 21396688 |
| evaluation of mammalian and intermediate host surveillance methods for detecting schistosomiasis reemergence in southwest china. | schistosomiasis has reemerged in china, threatening schistosomiasis elimination efforts. surveillance methods that can identify locations where schistosomiasis has reemerged are needed to prevent the further spread of infections. | 2011 | 21408127 |
| improvement in the diagnosis of brucella abortus infections in naturally infected water buffaloes (bubalus bubalis) using an elisa with a protein-g-based indicator system. | brucellosis caused by brucella abortus in domestic water buffaloes (bubalus bubalis) raised under the traditional system of husbandry in northern india was diagnosed using an enzyme-linked immunosorbent assays (elisa) with a protein-g-based indicator system (protein-g elisa). a total of 1,551 animals that are positive (n = 61), negative (n = 243), and suspected (n = 1,247) for brucellosis were examined. rose bengal test (rbt) was used to predict the disease, and accordingly, animals were dichoto ... | 2011 | 21442153 |
| effect of straw size and thawing time on quality of cryopreserved buffalo (bubalus bubalis) semen. | this study was designed to compare the effect of straw size (0.25 vs. 0.5 ml) and thawing time (30 vs. 60 sec) on the quality of cryopreserved buffalo bull semen. sperm motility, plasma membrane integrity and viability were higher (p = 0.05) in 0.25 ml than 0.5 ml straw, thawed at 37°c either for 30 or 60 sec. in conclusion, cryopreservation of buffalo semen in 0.25 ml straw resulted in a higher post-thaw quality. | 2011 | 21455280 |
| fluorescence in situ hybridization mapping of six loci containing genes involved in the dioxin metabolism of domestic bovids. | six loci containing genes involved in the dioxin metabolism (arnt, ahr, cyp1a1, cyp1a2, cyp1b1 and ahrr) were assigned, for the first time, to cattle (bos taurus, 2n = 60, bta), river buffalo (bubalus bubalis, 2n = 50, bbu), sheep (ovis aries, 2n = 54, oar) and goat (capra hircus, 2n = 60, chi) chromosomes by comparative fish-mapping and r-banding using bovine bac-clones. the following chromosome locations were found: arnt to bta3q21, bbu6q21, oar1p21 and chi3q21, ahr to bta4q15, bbu8q15, oar4q1 ... | 2011 | 21465154 |
| brucellosis in domestic water buffalo (bubalus bubalis) of trinidad and tobago with comparative epidemiology to cattle. | the water buffalo is an important domestic animal worldwide, and the local buffalypso variety was developed in trinidad to have improved beef qualities. brucellosis was diagnosed in trinidad and tobago during 1998 in both cattle and domestic water buffalo (bubalus bubalis) populations. brucellosis in the latter species is caused by infection with brucella abortus, similar to bovine brucellosis. control of brucellosis is of paramount importance to preservation of the genetic diversity of these an ... | 2011 | 21479842 |
| effects of various plant protein sources in high-quality feed block on feed intake, rumen fermentation, and microbial population in swamp buffalo. | this study was designed to determine effect of various plant protein sources in high-quality feed block (hqfb) on feed intake, rumen fermentation, and microbial population in swamp buffalo. four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly assigned according to a 4 × 4 latin square design. four kinds of plant protein sources (coarse rice bran (crb), cassava hay (ch), phaseolus calcaratus hay, and mulberry hay (mh)) were mixed in the hqfb. hqfbs were allowed to be licked at fr ... | 2011 | 21479843 |
| pregnancy rates following ai with sexed semen in mediterranean italian buffalo heifers (bubalus bubalis). | the use of sexed semen in farm animal production and genetic improvement has been shown to be feasible with variable degree of efficiency in a number of species, and proved to be economically viable in cattle. in the last two decades, various newly developed reproductive technologies applicable in buffaloes have mushroomed. recently, following the birth of the first buffalo calves using ai with sexed semen, commercial interest to exploit sexing of semen in this species too is aroused. in order t ... | 2011 | 21497388 |
| sarcocystis dubeyi (huong and uggla, 1999) infection in water buffaloes ( bubalus bubalis ) from egypt. | abstract water buffaloes ( bubalus bubalis ) are intermediate hosts for 4 species of sarcocystis , i.e., sarcocystis fusiformis and sarcocystis buffalonis with cats as definitive hosts; sarcocystis levinei with dogs as definitive hosts; and sarcocystis dubeyi with an unknown definitive host but thought to be zoonotic. currently, the latter species has been identified with certainty only from vietnam. in the present study, sarcocysts of s. dubeyi are reported in 11 (30%) of 35 egyptian water buff ... | 2010 | 21506768 |
| seroprevalence of neospora caninum in dairy buffaloes in lahore district, pakistan. | abstract seroprevalence of neospora caninum in dairy buffaloes ( bubalus bubalis ) was assessed in the lahore district of punjab province, pakistan. the study revealed an overall prevalence of 54.7% for n. caninum antibodies determined through a competitive enzyme-linked immunosorbent assay performed on randomly collected serum samples. the highest prevalence was observed in buffaloes >3-5 yr of age (64.1%), followed by 57.9% for 5 to 6 yr olds, and 55.8% in 1-yr-old neonates, with high probabil ... | 2011 | 21506867 |
| the "dark side" of β-lactoglobulin: unedited structural features suggest unexpected functions. | the in-depth characterization of water buffalo (wb) whey proteins based on chromatographic and mass spectrometric techniques revealed unexpected structural co- and post-translational modifications for β-lactoglobulin (β-lg). the residues lys(47) and lys(69) of β-lg were found to be lactosylated early, at the time of milking. thiol groups of β-lg underwent a dynamic sulfhydryl/disulfide exchange that is probably essential in accomplishing specific physiological requirements in which proteins may ... | 2011 | 21507408 |
| methanogen diversity in the rumen of indian surti buffalo (bubalus bubalis), assessed by 16s rdna analysis. | the methanogenic communities in buffalo rumen were characterized using a culture-independent approach of a pooled sample of rumen fluid from three adult surti buffaloes. buffalo rumen is likely to include species of various methanogens, so 16s rdna sequences were amplified and cloned from the sample. a total of 171 clones were sequenced to examine 16s rdna sequence similarity. about 52.63% sequences (90 clones) had ⩾90% similarity, whereas, 46.78% of the sequences (81 clones) were 75-89% similar ... | 2011 | 21507441 |
| characterization of sarcocystis fusiformis based on sequencing and pcr-rflp in water buffalo (bubalus bubalis) in iran. | four sarcocystis species, i.e., sarcocystis fusiformis and sarcocystis buffalonis with cats as definitive hosts, sarcocystis levinei with dogs as definitive host, and sarcocystis dubeyi with unknown definitive host, have previously been described from water buffalo (bubalus bubalis). the aim of the present study was genetic characterization of the causative agent(s) of water buffalo sarcocystosis in khuzestan province, western iran. rflp-pcr and partial sequence analysis of 18s rdna gene were us ... | 2011 | 21526403 |
| characterization of {beta}-lactoglobulin from buffalo (bubalus bubalis) colostrum and its possible interaction with erythrocyte lipocalin-interacting-membrane-receptor (limr). | lipocalins form a widespread class of proteins involved in the transport of weakly soluble vitamins, hormones or hydrophobic molecules. β-lactoglobulin (blg-col), a major lipocalin present in whey was purified and characterized from buffalo colostrum. the molecular weight of blg-col as determined by lc-esi ms was 18.257 kda and the peptide mass fingerprint of the purified protein revealed 67% sequence homology to buffalo milk β-lactoglobulin. the n-terminal-iivtq and lc-esi-cid/etd ms/ms analyse ... | 2011 | 21546361 |
| production of cloned and transgenic embryos using buffalo (bubalus bubalis) embryonic stem cell-like cells isolated from in vitro fertilized and cloned blastocysts. | abstract here, we report the isolation and characterization of embryonic stem (es) cell-like cells from cloned blastocysts, generated using fibroblasts derived from an adult buffalo (baf). these nuclear transfer embryonic stem cell-like cells (nt-es) grew in well-defined and dome-shaped colonies. the expression pattern of pluripotency marker genes was similar in both nt-es and in vitro fertilization (ivf) embryo-derived embryonic stem cell-like cells (f-es). upon spontaneous differentiation via ... | 2011 | 21548826 |
| substitutes for endangered medicinal animal horns and shells exposed by antithrombotic and anticoagulation effects. | cornu saigae tataricae (antelope horn), manis squama (pangolin scale), cornu cervi pantotrichum (velvet antler) and cornu bovis grunniens (yak horn) are valuable medicinal animal horns and shells (mahs). as the major source of biological agents and ethnodrugs, mahs show pretty good bioactivities. however, with the increased demand for mahs, some of the medicinal resources are endangered, and there has been a concomitant increase in the prevalence of adulterated or impostor mahs. it is of great s ... | 2011 | 21549826 |
| effect of cytoplasmic volume on developmental competence of buffalo (bubalus bubalis) embryos produced through hand-made cloning. | abstract this study examined the effects of cytoplasmic volume on the developmental competence of hand-made cloned buffalo embryos. two different cell types, that is, buffalo fetal fibroblast (bff) and buffalo embryonic stem (es) cell-like cells were taken as donor cell and fused with one, two, or three demicytoplasts to generate embryos with decreased, normal (control), and increased cytoplasmic volume. using bff as a nuclear donor, the cleavage rate was similar in all the groups (p > 0.05), bu ... | 2011 | 21563942 |
| isolation of glucosidase and phospholipase positive bacillus circulans on aloa medium. | aim: to report the growth of glucosidase and phospholipase positive bacteria on agar listeria according to ottaviani and agosti (aloa) different from listeria monocytogenes, listeria ivanovii and bacillus cereus. methods and results: raw water-buffalo milk was analysed according to en iso 11290. streaking of fraser broth on aloa resulted in green colonies with an opaque halo after 48 h at 30°c. colonies were transferred onto tryptone soya yeast extract agar and showed cultural characteristics ... | 2011 | 21575023 |
| effect of low density lipoproteins in extender on freezability and fertility of buffalo (bubalus bubalis) bull semen. | this study was designed to determine whether low-density lipoporoteins (ldls) extracted from egg yolk in extender improve the freezability and fertility of buffalo bull semen. semen from three nili-ravi buffalo bulls was diluted at 37 °c with tris-citric acid extender (50 × 10(6) motile spermatozoa ml(-1)) containing ldls 2.5%, 5%, 10%, and 15% extracted from egg yolk and extender containing 20% egg yolk was kept as control. diluted semen was cooled to 4 °c in 2 h, equilibrated at 4 °c for 4 h, ... | 2011 | 21601914 |
| molecular characterization and prevalence of cystic echinococcosis in slaughtered water buffaloes in turkey. | the present study was carried out between march 2006 and june 2010. during the study nine abattoirs were visited and 166 water buffalo internal organs were examined in black sea region of turkey. it was found that 10.24% buffaloes were infected with cystic echinococcosis (ce). the rate of ce found as 3.77% in males and 21.66% in females, 37.93% in older and 4.38% in young animals. the degree of prevalence according to age and sex was statistically significant (p<0.05). ce was observed 29.41% onl ... | 2011 | 21601988 |
| genetic parameters for milk, fat and protein yields in murrah buffaloes (bubalus bubalis artiodactyla, bovidae). | the objective of the present study was to estimate genetic parameters for test-day milk, fat and protein yields and 305-day-yields in murrah buffaloes. 4,757 complete lactations of murrah buffaloes were analyzed. co-variance components were estimated by the restricted maximum likelihood method. the models included additive direct genetic and permanent environmental effects as random effects, and the fixed effects of contemporary group, milking number and age of the cow at calving as linear and q ... | 2010 | 21637608 |
| microisolation and microcloning of bovine x-chromosomes for identification of sorted buffalo (bubalus bubalis) spermatozoa. | flow-cytometry sorting technology has been successfully used to separate the x- and y-chromosome bearing spermatozoa for production of sex-preselected buffalo. however, an independent technique should be employed to validate the sorting accuracy. in the present study, x-chromosomes of bovine were micro-dissected from the metaphase spreads by using glass needles. then x-chromosomes were then amplified by pcr and labelled with cy3-dutp for use as a probe in hybridization of the unsorted and sorted ... | 2011 | 21640528 |
| attenuation of luteolytic response following fish meal supplementation in dairy buffaloes (bubalus bubalis). | luteolysis of corpus luteum, due to un-inhibited pgf(2α) secretion, has been reported to be a cause of early embryonic mortality in dairy animals. the objective of this study was to determine the effects of fish meal (fm) supplementation on the uterine secretion of pgf(2α) and hence establish its supplementation as an antiluteolytic strategy in dairy buffaloes. five cycling murrah buffaloes were supplemented with 250g fm daily for 55 days in addition to their routine feed and seven buffaloes wer ... | 2011 | 21641133 |
| follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in water buffalo (bubalus bubalis). | the objective of this study was to examine the follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in buffaloes. ovaries were collected from 56 acyclic and 95 cyclic buffaloes at slaughter, surface follicle number was counted and follicles were classified into small (5.0-6.9 mm), medium (7.0-9.9 mm), and large (≥10.0 mm) size categories based on their diameter. follicular fluid was aspirated and assayed for nitric oxide, ascor ... | 2011 | 21643661 |
| characterization of enterotoxigenic e. coli (etec), shiga-toxin producing e. coli (stec) and necrotoxigenic e. coli (ntec) isolated from diarrhoeic mediterranean water buffalo calves (bubalus bubalis). | two hundred and twenty escherichia coli isolates from 314 mediterranean water buffalo calves less than 4weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors lt, st, stx1, stx2, haemolysins, intimin, cnf1, cnf2, cdt-i, cdt-ii, cdt-iii, cdt-iv, and f17-related fimbriae (f17a, f17b, f17c, f17d). the prevalence of etec, stec and ntec were 1.8%, 6.8% and 20.9%, respectively. the etec isolates were all lt-positive and st-negative. th ... | 2011 | 21658736 |
| molecular prevalence of different genotypes of theileria orientalis detected from cattle and water buffaloes in thailand. | abstract here, we report on an epidemiological study regarding the molecular prevalence of different genotypes of theileria orientalis present among domestic cattle and water buffalo populations bred in thailand. a phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein (mpsp) revealed the presence of 5 genotypes (types 1, 3, 5, 7, and n-3) in cattle and 7 genotypes (types 1, 3, 4, 5, 7, n-2, and n-3) in water buffaloes. types 4, 7, and n-3 of t. orientalis w ... | 2011 | 21671722 |
| effect of cryopreservation on apoptotic-like events and its relationship with cryocapacitation of buffalo (bubalus bubalis) sperm. | this study investigated the apoptosis-like events associated with cryopreservation process and their relationship with cryocapacitation in buffalo (bubalus bubalis) sperm. a total of 49 semen ejaculates from seven bulls were studied for structural changes in sperm following cryopreservation. apoptotic changes were detected by assays specific for translocation of phosphatidylserine (ps) to the cell surface, alterations in membrane permeability and mitochondrial membrane potential (mmp), and dna i ... | 2011 | 21676035 |
| clinical, hematological, and biochemical findings of uterine torsion in buffaloes (bubalus bubalis). | the aim of this study was to investigate uterine torsion in buffaloes, examine factors influencing the outcome of the disease, and to characterize the related alterations in blood constituents. a total of 126 buffaloes with uterine torsion were examined for stage of gestation, duration, degree, site and direction of torsion, as well as the location of the pregnant horn. methods of correction were documented along with dam and calf survival. blood samples were obtained from 20 buffaloes with uter ... | 2011 | 21676564 |
| genotyping of the k-casein and β-lactoglobulin genes in chinese holstein, jersey and water buffalo by pcr-rflp. | 2011 | 21677408 | |
| development and evaluation of real-time pcr assay for the detection of babesia orientalis in water buffalo (bubalus bubalis, linnaeus, 1758). | abstract babesia orientalis is the causative agent of babesiosis in water buffalo (bubalus babalis, linnaeus, 1758). in this study, a taqman real-time pcr assay was developed for quantitative detection of b. orientalis in water buffalo. hybridization probe and oligonucleotide primers were designed based on the v4 region of 18s rrna gene. detection limit was determined at 2 parasites. blood samples were collected from experimentally infected water buffalo, as well as from 180 field samples, which ... | 2011 | 21711103 |
| rsai repetitive dna in buffalo bubalus bubalis representing retrotransposons, conserved in bovids, are part of the functional genes. | abstract: | 2011 | 21718551 |
| constant transmission of mitochondrial dna in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm. | although interspecies/intergeneric somatic cell nuclear transfer (iscnt) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iscnt embryos has been identified as an impediment to implementation for agricultural production. to investigate the nuclear-mitochondrial interactions on the developmental potential of iscnt embryos, we analyzed the mtdna copy numbers in iscnt embryos reconstructed with water buffalo (swamp type ... | 2011 | 21729201 |
| behaviour of listeria monocytogenes in packaged water buffalo mozzarella cheese. | aims:ôçé the aim of the study was to evaluate the behaviour of listeria monocytogenes in the conditioning liquid of packaged water buffalo mozzarella cheese (wbmc). methods and results:ôçé the conditioning liquid was contaminated with l.-ámonocytogenes, and the contaminated samples were stored at four different storage temperatures: 5 and 10-¦c for 22ôçâdays; 20-¦c for 9ôçâdays; 20-¦c for 3ôçâdays and then at 5-¦c for 6ôçâdays. the results showed that l.-ámonocytogenes concentration decreased wh ... | 2011 | 21740447 |
| genetic differentiation of water buffalo (bubalus bubalis) populations in china, nepal and south-east asia: inferences on the region of domestication of the swamp buffalo. | data from three published studies of genetic variation at 18 microsatellite loci in water buffalo populations in china (18 swamp type, two river type), nepal (one wild, one domestic river, one hybrid) and south-east asia (eight swamp, three river) were combined so as to gain a broader understanding of genetic relationships among the populations and their demographic history. mean numbers of alleles and expected heterozygosities were significantly different among populations. estimates of ? (a me ... | 2011 | 21749419 |
| polymorphism and gene organization of water buffalo mhc-dqb genes show homology to the bola dqb region. | in cattle (bos taurus), there is evidence of more than 50 alleles of bola-dqb (bovine lymphocyte antigen dqb) that are distributed across at least five dqb loci, making this region one of the most complex in the bola gene family. in this study, dqb alleles were analysed for the water buffalo (bubalus bubalis), another economically important bovine species. twelve alleles for bubu-dqb (bubalis bubalis dqb) were determined by nucleotide sequence analysis. a phylogenetic analysis revealed numerous ... | 2011 | 21749420 |
| genetic diversity of mitochondrial cytochrome b gene in chinese native buffalo. | the origins of the domestic water buffalo remain contentious. to better understand the origins of chinese water buffalo, we sequenced the complete mitochondrial cytochrome b (mt-cyb) gene from 270 individuals representing 13 chinese domestic swamp buffalo populations. we found genetic evidence of introgression of river buffalo into chinese swamp buffalo herds. swamp buffalo haplotypes can be divided into two highly divergent lineages (a and b), suggesting that chinese native swamp buffalo have t ... | 2011 | 21749426 |
| maturation competence of swamp buffalo oocytes obtained by ovum pick-up and from slaughterhouse ovaries. | this study was designed with the final goal of improving in vitro embryo production in the thai swamp buffalo (bubalus bubalis carabensis). oocytes were collected by ovum pick-up (opu) from six non-lactating multiparous swamp buffalo twice per week for 10 consecutive sessions followed by once-weekly collection for 10 consecutive sessions without hormone stimulation. in addition, oocytes were collected from slaughterhouse ovaries that were classified as follows: ovaries from non-pregnant cows wit ... | 2011 | 21749483 |
| effects of recipient oocyte age and interval from fusion to activation on development of buffalo (bubalus bubalis) nuclear transfer embryos derived from fetal fibroblasts. | the objective was to investigate the effect of recipient oocyte age and the interval from activation to fusion on developmental competence of buffalo nuclear transfer (nt) embryos. buffalo oocytes matured in vitro for 22 h were enucleated by micromanipulation under the spindle view system, and a fetal fibroblast (pretreated with 0.1 µg/ml aphidicolin for 24 h, followed by culture for 48 h in 0.5% fetal bovine serum) was introduced into the enucleated oocyte, followed by electrofusion. both oocyt ... | 2011 | 21752448 |
| enhancing reproductive performance in domestic dairy water buffalo (bubalus bubalis). | the purpose of the review is to describe the factors that affect fertility in domestic water buffalo (bubalus bubalis) and the techniques that enable an improvement in reproductive performance. on italian and latin american farms where natural mating is practiced and bulls are always present in the herd, the inter-calving interval is approximately 400 days and the culling rate is lower than 15%. the buffalo has a tendency for seasonal reproductive activity. reproduction is favoured when there is ... | 2010 | 21755690 |
| sequence characterization of s100a8 gene reveals structural differences of protein and transcriptional factor binding sites in water buffalo and yak. | the present study was undertaken to characterize the structure of s100a8 gene and its promoter in water buffalo and yak. sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete s100a8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. blast analysis of coding dna sequences (cds) of s100a8 gene revealed 95% homology of buffalo sequence with cattle, 85% with pig and horse, 83% with dog, 72-73% with murines, and around 79% with ... | 2011 | 21774620 |
| somatotropin-mediated gene expression profiling of differentially displayed ests during lactation in indian buffalo (bubalus bubalis). | the study of bovine mammary gland functional genomics requires appropriate cdna library collections to access gene expression patterns from different developmental and physiological stages. the present study was undertaken with the objective to identify candidate genes involved in the process of increased milk synthesis following 0, 48 and 96 h of recombinant bovine somatotropin (rbst) treatment to surti buffalo (bubalus bubalis) through differential display reverse transcriptase pcr (ddrt-pcr). ... | 2011 | 21774858 |
| in vitro immune competence of buffaloes (bubalus bubalis) of different production potential: effect of heat stress and cortisol. | twelve healthy lactating murrah buffaloes of similar parity (3rd) between 90 and 120 days of lactation, selected from the herd of national dairy research institute (karnal, india) and maintained at managemental practices as followed at the institute they were included in this experiment. the animals were divided into two groups based on their production level in previous lactation. the average milk production level of group 1 and ii was 9.3 and 6?lit/day, respectively. blood was collected from t ... | 2011 | 21776354 |
| in vivo changes in antioxidant system and protective role of selenium in chlorpyrifos-induced subchronic toxicity in bubalus bubalis. | chlorpyrifos, an organophosphate, is one of the widely used insecticides for control of pests in various agricultural and animal husbandry operations. the objective of the present investigation was to assess the effect of subchronic exposure of chlorpyrifos on the antioxidant status of buffalo calves and to perceive the role of selenium in cases of chlorpyrifos toxicity. chlorpyrifos at a dose rate of 0.05mg/kg per day for 20 consecutive weeks, significantly elevated the enzymic activity of glut ... | 2008 | 21783886 |
| presence of nitric oxide synthase immunoreactivity and mrna in buffalo (bubalus bubalis) oocytes and embryos. | this study examined the presence of immunoreactivity and mrna for different nitric oxide synthase (nos) isoforms in immature and in vitro matured oocytes and in embryos at two-, four- and eight-cell, and morula and blastocyst stages in buffalo. oocytes obtained from slaughterhouse buffalo ovaries were subjected to in vitro maturation in tcm-199 + 10% fbs + 5 µg/ml pfsh + 1 µg/ml estradiol-17ß + 0.81 mm sodium pyruvate + 10% buffalo follicular fluid + 50 µg/ml gentamycin sulphate for 24 h in a co ... | 2011 | 21793945 |
| comparative evaluation of halothane anaesthesia in medetomidine-butorphanol and midazolam-butorphanol premedicated water buffaloes (bubalus bubalis). | six clinically healthy male water buffaloes (bubalus bubalis) 2-3 years of age and weighing 290-325 kg were used for 2 different treatments (h1 and h2). the animals of group h1 were premedicated with medetomidine (2.5 g/kg,i.v.) and butorphanol (0.05 mg/kg, i.v.), while in group h2 midazolam (0.25 mg/kg) and butorphanol (0.05 mg/kg) were used intravenously. induction of anaesthesia was achieved by 5% thiopental sodium in h1 (3.85 +/- 0.63 mg/kg) and h2 (6.96 +/- 0.45 mg/kg) groups. the anaesthes ... | 2011 | 21826832 |
| changes in biochemical composition of follicular fluid during reproductive acyclicity in water buffalo (bubalus bubalis). | this study describes the changes in biochemical composition of follicular fluid during reproductive acyclicity in buffalo. a total of 73 pairs of ovaries collected from 26 reproductively acyclic and 47 reproductively cyclic buffaloes were used in the investigation. ovarian follicles were classified into small (5.0-6.9mm), medium (7.0-9.9mm) and large (ôëñ10.0mm) sized categories depending upon their diameter. follicular fluid was aspirated, processed and assayed for glucose, cholesterol, total p ... | 2011 | 21840653 |
| hormone variations in serum and milk of buffaloes (bubalus bubalis) as potential indicators of treatment with recombinant bovine somatotropin. | recombinant bovine somatotropin (rbst) is used to increase milk yield in cows, but it has been forbidden in some countries and in the eu. however, rbst misuse represents a concern in both bovine and buffalo dairy production. a number of studies on rbst treatment have been performed on bovines, but there are few data on buffaloes. in this study, we treated eight lactating buffaloes with biweekly injections of a slow-release formulation of rbst, for five cycles of administration, and analysed tota ... | 2011 | 21843393 |
| domestic livestock resources of turkey : water buffalo. | water buffalo are an ancient component of turkey's domestic livestock resources. commonly referred to as the anatolian buffalo the animal is part of the mediterranean group which includes syrian, egyptian and southeast european animals. once quite numerous, there have been drastic reductions in their numbers since the 1970s due to intensification of dairy activities, agricultural mechanization and changing consumer preferences. the main areas of distribution are in northwest turkey in the marmar ... | 2011 | 21870064 |
| Ovum pick-up and in vitro embryo production (OPU-IVEP) in Mediterranean Italian buffalo performed in different seasons. | This study was designed to evaluate the effect of season on in vivo oocyte recovery and embryo production in Mediterranean Italian buffalo (Bubalus bubalis). For this purpose repeated transvaginal ultrasound-guided ovum pick up (OPU) was conducted twice a week throughout autumn, mid-winter (transitional period) and spring-summer. The number and size of follicles was determined before puncture. The recovered oocytes were first classified in morphological categories and then used for in vitro embr ... | 2012 | 21872310 |
| DGAT1, GH, GHR, PRL and PRLR Polymorphism in Water Buffalo (Bubalus bubalis). | The polymorphism of several genes has been shown to affect the milk composition traits in dairy cattle, including DGAT1-exon8 K232A, GH-intron3 MspI, GH-exon5 AluI, GHR-exon8 F279Y, PRL-exon3 RsaI and PRLR-exon3 S18N. However, the polymorphism and effects of these genes on the milk traits of water buffalo are still unclear. In this study, four DNA pooling samples from Murrah, Nili-ravi, Murrah-Nili-Swamp crossbreed and Chinese swamp buffalo were constructed, respectively, and polymorphism of the ... | 2011 | 21883511 |
| methyl coenzyme m reductase (mcra) gene based phylogenetic analysis of methanogens population in murrah buffaloes (bubalus bubalis). | the aim of the present study was to decipher the diversity of methanogens in rumen of murrah buffaloes so that effective strategies can be made in order to mitigate methane emission from these methanogens. in the present study diversity of rumen methanogens in murrah buffaloes (bubalus bubalis) from north india was evaluated by using mcr-a gene library obtained from the pooled pcr product from four animals and by using mega4 software. a total of 104 clones were examined, revealing 26 different m ... | 2011 | 21887637 |
| Clinical and haematological study on water buffaloes (Bubalus bubalis) and crossbred cattle naturally infected with Theileria annulata in Sharkia province, Egypt. | This study was conducted to investigate the clinical and haematological findings in water buffaloes and crossbred cattle naturally infected with Theileria annulata with special reference to the clinical picture of tropical theileriosis in Egyptian buffaloes. A total 50 field cases of buffaloes and cattle was clinically and laboratory investigated from March to June 2008. Forty-four buffaloes and cattle out of 50 were naturally infected with T. annulata and showed typical signs of infection. Six ... | 2011 | 21890072 |
| influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (bubalus bubalis) preantral follicles. | effect of sodium nitroprusside (snp), a nitric oxide (no) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (pfs) was investigated. pfs (200~250 µm) were isolated by micro-dissection and cultured in 0 (control), 10(-3), 10(-5), 10(-7), and 10(-9) m snp. to examine the reversible effect of snp, pfs were cultured with 10(-5) m snp + 1 mm n(ω)-nitro-l-arginine methyl ester (l-name) or 1.0 µg hemoglobin (hb). the results showed that greater concentrat ... | 2011 | 21897099 |
| buffalo (bubalus bubalis) epiphyseal proteins give protection from arsenic and fluoride-induced adverse changes in acetylcholinesterase activity in rats. | the objective of this study was to determine the effect of fluoride (f) and arsenic (as) on the activity of acetylcholinesterase (ache), a critically important nervous system enzyme, and to test the protective role of buffalo epiphyseal (pineal) proteins (bep) in rats. arsenic (20 mg/kg bw, intraperitoneally) and f (150 ppm, perorally) were exposed, and bep was administered intraperitoneally (100 μ g/kg bw) along with f and as to rats for 7 days. as and f exposure significantly (p < 0.05) increa ... | 2011 | 21898718 |
| Congenital hydranencephaly and cerebellar hypoplasia in water buffalo in southern Brazil. | Seven cases of hydranencephaly and cerebellar hypoplasia in a water buffalo herd are described. The herd of 133 females was divided for mating into 4 groups and mated with different bulls. The disease was observed in the offspring of 5 cows from only 1 group over a period of 6 years. These cows had all mated with the same bull during that period. All affected calves were unable to suckle, showed intention tremors, involuntary movements of the ears, depression, and blindness. Some calves were rec ... | 2011 | 21908300 |
| baccharis megapotamica var. weirii poisoning in water buffalo (bubalus bubalis). | an outbreak of an acute disease in buffalo (bubalus bubalis) caused by the ingestion of baccharis megapotamica var. weirii occurred in the southern region of brazil. ten out of 50 buffalo died 24-48 hr after being introduced into a pasture containing abundant amounts of the plant. factors influencing the ingestion of the plant and consequent toxicosis included hunger, stress caused by shipment, and unfamiliarity with the plant. clinical signs included serous ocular discharge, incoordination, mil ... | 2011 | 21908301 |
| expression of nitric oxide synthase isoforms in different stages of buffalo (bubalus bubalis) ovarian follicles: effect of nitric oxide on in vitro development of preantral follicle. | the present study was designed to investigate the expression of nitric oxide synthase (nos) isoforms in buffalo ovarian preantral (pfs), antral (afs) and ovulatory (ofs) follicles (experiment 1); effect of no on in vitro survival and growth of pfs (experiment 2) and nos activity in immature oocytes by nadph-diaphorase test (experiment 3). in experiment 1, nos isoforms (neuronal, inducible and endothelial) were localized immunohistochemically; mrna and protein expression was analyzed by semi-quan ... | 2011 | 21924465 |
| effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of handmade cloned buffalo (bubalus bubalis) embryos. | the aim of the present study was to investigate the effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of handmade cloned (hmc) and parthenogenetically activated (pa) buffalo embryos. scriptaid treatment was given for 10 h immediately after reconstruction of hmc and pa embryos. treatment of hmc embryos with 500 or 1000 nmol/l scriptaid significantly increased (p<0.01) the cleavage rate (91.3±2.8 and 91.9±2.0%, respectively) than that of controls (80.7±2.4%). bl ... | 2012 | 21924478 |
| a comparative study on mimosine, 3,4-dihydroxy pyridone (3,4-dhp) and 2,3-dihydroxy pyridone (2,3-dhp), purine derivatives (pd) excretion in the urine, thyroid hormone and blood metabolites profiles of thai swamp buffalo (bubalus bubalis) and murrah buffalo (bubalus bubalis). | four thai swamp buffaloes (sb) and four murrah buffaloes (mb) fed a based diet of fresh ruzi grass (bachiaria ruziziensis) with an increased proportion of fresh leucaena leaves. intake of nutrients in animals increased when ruzi grass was mixed with leucaena. digestibility of nutrients were the highest in sb and mb fed diets containing 25% and 50% of leucaena, respectively, and nitrogen (n) balances in both animal breeds were varied among diets. the regression equation coefficient of mimosine + ... | 2011 | 21928129 |
| effect of selenium, zinc, and copper supplementation on blood metabolic profile in male buffalo (bubalus bubalis) calves. | twenty male buffalo calves (15 months, 200.2 ± 9.75) were divided into four groups of five animals in each and fed diets without (t1) or supplemented with 0.3 ppm selenium (se) + 40 ppm zinc (zn) (t2), 0.3 ppm se + 40 ppm zn + 10 ppm copper (cu) (t3), and 40 ppm zn + 10 ppm cu (t4) for 120 days, during which blood samples were collected on days 0, 40, 80, and 120. concentrations of glucose, total protein, albumin, globulin, urea, uric acid, and creatinine were similar in all the four groups. the ... | 2011 | 21947859 |
| Metagenomic analysis of Surti buffalo (Bubalus bubalis) rumen: a preliminary study. | The complex microbiome of the rumen functions as an effective system for the conversion of plant cell wall biomass to microbial proteins, short chain fatty acids and gases. In this study, metagenomic approaches were used to study the microbial populations and metabolic potential of the microbial community. DNA was extracted from Surti Buffalo rumen samples (four treatments diet) and sequenced separately using a 454 GS FLX Titanium system. We used comparative metagenomics to examine metabolic pot ... | 2011 | 21947953 |
| Molecular mining of alleles in water buffalo Bubalus bubalis and characterization of the TSPY1 and COL6A1 genes. | Minisatellites are an integral part of eukaryotic genomes and show variation in the complexity of their organization. Besides their presence in non-coding regions, a small fraction of them are part of the transcriptome, possibly participating in gene regulation, expression and silencing. We studied the minisatellite (TGG)(n) tagged transcriptome in the water buffalo Bubalus bubalis across various tissues and the spermatozoa, and characterized the genes TSPY1 and COL6A1 discovered in the process. | 2011 | 21949806 |
| translocation of active mitochondria during buffalo (bubalus bubalis) oocytes in vitro maturation, fertilization and preimplantation embryo development. | mitochondria are energy-supplying organelles, whose distribution and functional integrity are necessary for cell survival and development. in this study, the mitochondrial distribution pattern and activity during buffalo oocyte in vitro maturation, fertilization and preimplantation embryo development were revealed using a fluorescent dye and confocal laser scanning microscopy. distribution of active mitochondria changed during buffalo oocyte in vitro maturation. active mitochondria were transfer ... | 2011 | 21950622 |
| Semi-quantitative RT-PCR analysis of fat metabolism genes in mammary tissue of lactating and non-lactating water buffalo (Bubalus bubalis). | Understanding the mechanism of milk fat synthesis and secretion is important for dairy industry, as the nature of the cream fraction influences the manufacturing properties and organoleptic qualities of milk and dairy products. So, there is a need to understand the mechanism of milk fat synthesis and to elucidate the key genes regulating milk fat synthesis by studying the expression of genes involved in milk fat synthesis. Present manuscript reports the expression of genes involved in milk fat s ... | 2011 | 21965031 |
| In search of the genetic footprints of Sumerians: a survey of Y-chromosome and mtDNA variation in the Marsh Arabs of Iraq. | For millennia, the southern part of the Mesopotamia has been a wetland region generated by the Tigris and Euphrates rivers before flowing into the Gulf. This area has been occupied by human communities since ancient times and the present-day inhabitants, the Marsh Arabs, are considered the population with the strongest link to ancient Sumerians. Popular tradition, however, considers the Marsh Arabs as a foreign group, of unknown origin, which arrived in the marshlands when the rearing of water b ... | 2011 | 21970613 |
| Isolation, culturing and characterization of feeder-independent amniotic fluid stem cells in buffalo (Bubalus bubalis). | Heterogeneous amniotic fluid contains various cell types. The aim of this study was to characterize and differentiate some of the key stemness attributes of the amniotic fluid-derived cells in buffalo (Bubalus bubalis). The amniotic fluid (AF) cells were cultured without feeder cells, in DMEM containing 15% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin/ampicillin, 1% vitamin solution, and 1% l-glutamine in 5% CO(2) in humidified air at 38.5±0.5°C. After 6days of culture different ... | 2011 | 21982462 |
| assessment of intracellular ca(2+) , camp and 1,2-diacylglycerol in cryopreserved buffalo (bubalus bubalis) spermatozoa on supplementation of taurine and trehalose in the extender. | in mammalian spermatozoa, intracellular calcium plays a major role in sperm functions like motility and capacitation. cryopreservation-induced modifications to sperm membrane result in an influx of intracellular calcium affecting calcium-dependent intracellular signalling pathways. intracellular calcium activates adenyl cyclase to produce camp that activates phospholipase a(2) (pla(2) ) and phospholipase c (plc) generating lysophosphatidyl choline, 1,2-diacylglycerol (dag) and ip(3) , acting as ... | 2011 | 21988572 |
| common occurrence of a unique cryptosporidium ryanae variant in zebu cattle and water buffaloes in the buffer zone of the chitwan national park, nepal. | there are very few studies on the diversity and public health significance of cryptosporidium species in zebu cattle and water buffaloes in developing countries. in this study, pcr-restriction fragment length polymorphism and dna sequence analyses of the small-subunit (ssu) rrna gene were used to genotype cryptosporidium specimens from 12 zebu cattle calves, 16 water buffalo calves, and four swamp deer (cervus duvaucelii) collected from the buffer zone of the chitwan national park, nepal. all cr ... | 2011 | 21996006 |
| Morphological characteristics of Echinococcus granulosus derived from buffalo in Iran. | SUMMARYCystic echinococcosis is a significant parasitic disease in Iran, where a variety of animals act as intermediate hosts. In this study, 25 isolates of Echinococcus granulosus obtained from water buffalo from various parts of Iran were characterized on the basis of the morphology of the metacestode and the adult worm. The characteristics of protoscoleces from the different studied areas were nearly similar. They showed 2 rows of alternating large and small hooks and their shapes were smooth ... | 2011 | 21996421 |
| molecular modeling and docking characterization of dectin-1 (pamp) receptor of bubalus bubalis. | dectin-1, is a type ii transmembrane receptor protein which contains a single extracellular ctld (c-type lectin domain), stalk, transmembrane domain and an itam (immunoreceptor tyrosine-based activation motifs) in its cytoplasmic tail. dectin-1 has the ability to recognize fungal β-glucans, which are carbohydrate pamps found predominantly in fungal cell walls. the recognition of fungal β-glucans by dectin-1 helps in a variety of cellular responses, like host protection, such as fungal uptake and ... | 2011 | 22015804 |
| Comparative Quality Assessment of Buffalo (Bubalus bubalis) Semen Chilled (5°C) in Egg Yolk- and Soya Milk-Based Extenders. | Egg yolk-Tris is most commonly used semen extender; however, its use involves hygienic risk, interference with fertility and poor microscopic examination. Therefore, replacement of egg yolk with a plant-based component with protective effects on spermatozoa would be advantageous. In present study, we observed effect of soya milk-based extenders on dilution and liquid preservation of Murrah buffalo bull semen at 5°C up to 72 h in comparison with conventional egg yolk-Tris extender (Ext.1). In exp ... | 2011 | 22017209 |
| characterization of pou5f1 (oct4) gene and its promoter in buffalo esc-like cells identifies multiple transcription start sites and expression of four pseudogenes. | in the present study, we cloned and characterized the buffalo (bubalus bubalis) oct4 ortholog expressed in embryonic stem cell (esc) like cells and its promoter region. the 5'- and 3'-race experiments were conducted to analyze the transcription initiation site and regulatory regions. the comparative analysis of buffalo oct4 promoter with other mammalian orthologs revealed high conservation. among the regulatory regions highest similarity was observed between buffalo, bovine and sheep. interestin ... | 2012 | 22019428 |
| Optimization of Culture Conditions to Support Long-Term Self-Renewal of Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells. | Abstract A culture system capable of sustaining self-renewal of buffalo embryonic stem (ES) cell-like cells in an undifferentiated state over a long period of time was developed. Inner cell masses were seeded on KO-DMEM + 15% KO-serum replacer on buffalo fetal fibroblast feeder layer. Supplementation of culture medium with 5?ng/mL FGF-2 and 1000?IU/mL mLIF gave the highest (p<0.05) rate of primary colony formation. The ES cell-like cells' colony survival rate and increase in colony size were h ... | 2011 | 22029416 |
| Short communication: Translational efficiency of casein transcripts in Mediterranean river buffalo. | Buffalo milk is characterized by the presence of all 4 casein fractions (a(S1), ß, a(S2), and ?) encoded by the 4 tightly linked autosomal genes (CSN1S1, CSN2, CSN1S2, and CSN3, respectively). In the present paper, we report for the first time a quantitative characterization of buffalo casein transcripts and show that the 4 genes are not transcribed and translated with the same efficiency. In particular, the analysis of individual milk samples obtained from 9 Mediterranean river buffaloes showed ... | 2011 | 22032393 |
| identification of no induced and capacitation associated tyrosine phosphoproteins in buffalo (bubalus bubalis) spermatozoa. | to acquire the fertilizing competence, spermatozoa must undergo a cascade of physiological and biochemical changes collectively defined as capacitation. compelling evidence signifies that the global increase in protein tyrosine phosphorylation is the driving factor for capacitation. in our laboratory, we previously demonstrated that nitric oxide (no) induces capacitation in buffalo sperm and is associated with an increase in protein tyrosine phosphorylation. the aim of the present study is to id ... | 2011 | 22035659 |
| molecular mining of ggaa tagged transcripts and their expression in water buffalo bubalus bubalis. | repeat sequences are involved in regulation of gene expression both at the transcriptional and translational level. in the mammalian genomes, tri- and tetranucleotide repeats like ata, aata, ggaa and gaaa have been associated with diseases. in silico analysis of (ggaa)(5) distribution across the species showed maximum number of this repeat in the mouse transcriptome compared to that in other species. following this, we conducted minisatellite associated sequence amplification (masa) to explore t ... | 2012 | 22037482 |
| Multilocus phylogeographical analysis of Trypanosoma (Megatrypanum) genotypes from sympatric cattle and water buffalo populations supports evolutionary host constraint and close phylogenetic relationships with genotypes found in other ruminants. | Species of the subgenus Trypanosoma (Megatrypanum) have been reported in cattle and other domestic and wild ruminants worldwide. A previous study in Brazil found at least four genotypes infecting cattle (Bos taurus), but only one in water buffalo (Bubalus bubalis). However, the small number of isolates examined from buffalo, all inhabiting nearby areas, has precluded evaluation of their diversity, host associations and geographical structure. To address these questions, we evaluated the genetic ... | 2011 | 22051399 |
| fatty acid composition of water buffalo meat. | the fatty acid composition of intramuscular lipids of longissimus dorsi (ld), psoas major (pm), biceps femoris (bf), semitendinosus (st) muscles and liver of water buffalo male calves was determined by capillary gas-liquid chromatography. the content of total lipids in the ld muscle was found to be maximum, followed by pm, bf and st in decreasing order (1·03, 0·99, 0·66 and 0·55g/100g of fresh muscle). liver contained 2·65 g of total lipids per 100 g of fresh tissue. following the anatomical loc ... | 1986 | 22054932 |