Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a case of disseminated intravascular coagulation caused by brucella melitensis. | although the haematological abnormalities were common accompaniments of brucellosis, overt disseminated intravascular coagulopathy was reported only in a case at the literature. we report here a case of brucella melitensis sepsis which showed an acute onset with clinical and hematological findings disseminated intravascular coagulopathy | 2008 | 17562127 |
| the three-dimensional crystal structure of the prpf protein of shewanella oneidensis complexed with trans-aconitate: insights into its biological function. | in bacteria, the dehydration of 2-methylcitrate to yield 2-methylaconitate in the 2-methylcitric acid cycle is catalyzed by a cofactor-less (prpd) enzyme or by an aconitase-like (acnd) enzyme. bacteria that use acnd also require the function of the prpf protein, whose function was previously unknown. to gain insights into the function of prpf, the three-dimensional crystal structure of the prpf protein from the bacterium shewanella oneidensis was solved at 2.0 a resolution. the protein fold of p ... | 2007 | 17567742 |
| validation of a competitive elisa for diagnosis of brucella melitensis infection in sheep and goats. | a competitive enzyme-linked immunosorbent assay (celisa) was validated for the serodiagnosis of brucella melitensis infection in small ruminants using 2108 positive and 2154 negative reference sera from sheep and goats. the optimum cut-off values, offering the highest diagnostic sensitivity (dsn) and diagnostic specificity (dsp), determined by receiver operating characteristic analysis, were at 23.6%, 21.8% and 25.0% inhibition of the conjugate control for sheep, goats and both species, respecti ... | 2008 | 17574884 |
| in vitro activity of eight fluoroquinolones against clinical isolates of brucella melitensis. | since fluoroquinolones have generated much interest because of their excellent in vitro and in vivo activity, their pharmacokinetic propertiesm oral dosing and intracellular penetration, we tested the susceptibility of 146 recent clinical isolated of brucella melitensis agaist eight 4-quinolone drugs and five conventional drugs. the drugs tested included ciprofloxacin, norfloxacin, temafloxacin, sparfloxacin, fleroxacin, pefloxacin, lomefloxacin, ci-960, tetracycline, gentamicin, streptomycin ri ... | 1993 | 17587989 |
| ci-990 (pd 131112): a new quinolone prodrug. | in vitro susceptibility of 1021 strains of recent clinical isolates was determined against the new fluoroquinolone ci-990 (pd 131112) and compared with ci-960, ciprofloxacin, fleroxacin, lomefloxacin, norfloxacin, perfloxacin, sparfloxacin, and temafloxacin. the minimum inhibitory concentrations (mic) of ci-990 in mg/l required for >90% isolates were 0.03-0.5 for members of enterobacteriaceae, 0.12-2.0 for pseudomonas aeruginosa, 0.03-1.0 for aeromonas hydrophilia, 1.0-2.0 for acinetobacter calc ... | 1993 | 17588022 |
| congenital brucellosis: a rare cause of respiratory distress in neonates. | brucellosis represents a rare cause of neonatal infection. in this article we report a very unusual case of congenital infection due to brucella melitensis in a term neonate presenting after birth with severe respiratory distress and radiological manifestations (lobar consolidation and diffuse interstitial infiltrations) compatible with pulmonary involvement. the neonate was successfully treated with trimethoprim-sulfamethoxazole, rifampicin, and gentamicin. | 2007 | 17597440 |
| a dna vaccine coding for the chimera blsomp31 induced a better degree of protection against b. ovis and a similar degree of protection against b. melitensis than rev.1 vaccination. | in the present study, we reported an attempt to improve the immunogenicity and protective capacity of the chimera blsomp31 using a different antigen delivery: dna vaccination. vaccination of balb/c mice with the dna vaccine coding for the chimera blsomp31 (pciblsomp31) provided the best protection level against brucella ovis, which was significantly higher than the given by the co-delivery of both plasmids coding for the whole proteins (pcdnabls+pciomp31) and even higher than the control vaccine ... | 2007 | 17600596 |
| seroprevalence of and risk factors for brucellosis of goats in herds of michoacan, mexico. | our objectives were to estimate the seroprevalence of brucella melitensis, and to identify some risk factors associated with goat seropositivity in michoacan, mexico. blood samples were collected from 5114 animals from 79 herds. sera were tested for antibodies against b. melitensis using the rose bengal plate test and the complement-fixation test. information regarding the herds and each animal sampled were recorded through a personal interview at the farm. we used random-effects multivariable l ... | 2007 | 17604858 |
| hepatic abscess: a rare manifestation of brucellosis in children. | hepatic abscess caused by brucellosis is extremely rare in children. we report the case of a 5-year-old girl in whom an abscess of the liver developed during an episode of acute brucellosis. to our knowledge, this is the second reported case of hepatic abscess caused by brucellosis in a child. | 2008 | 17618456 |
| molecular epidemiological and antibiotic susceptibility characterization of brucella isolates from humans in sicily, italy. | brucellosis is a serious problem in sicily. brucella melitensis was identified as the species most frequently isolated in humans in italy. no data, however, are available about the molecular epidemiological characterization of brucella isolates from humans. we have conducted this study to molecularly characterize clinical isolates of brucella spp. and to evaluate their antimicrobial susceptibilities. twenty brucella isolates were studied. differential growth characteristics and dna polymorphisms ... | 2007 | 17634297 |
| valve replacement for brucella endocarditis: two case reports. | we report two cases of successful treatment of brucella endocarditis. both of them were treated with antibiotics and aortic valve replacement after brucellosis was diagnosed. in one of these cases emergency operation was required. our observations suggest that a combined surgical and medical treatment is the best option for the management of this disease. b. endocarditis should be operated after improvement of clinical status but emergency cardiac surgery may be required if heart failure develop ... | 2008 | 17651833 |
| aerosol infection of balb/c mice with brucella melitensis and brucella abortus and protective efficacy against aerosol challenge. | brucellosis is a zoonotic disease with a worldwide distribution that can be transmitted via intentional or accidental aerosol exposure. in order to engineer superior vaccine strains against brucella species for use in animals as well as in humans, the possibility of challenge infection via aerosol needs to be considered to properly evaluate vaccine efficacy. in this study, we assessed the use of an aerosol chamber to infect deep lung tissue of mice to elicit systemic infections with either bruce ... | 2007 | 17664263 |
| brucellar epididymoorchitis - report of five cases. | we report here 5 bacteriologically proven cases of brucellar epididymoorchitis. four cases presented with unilateral epididymoorchitis and with bilateral presentation in one case. blood culture grew brucella melitensis in all 5 cases. b.melitensis was isolated in testicular aspirate of 4 patients. brucella agglutinins were demonstrated in testicular aspirate of 4 patients and semen of 2 patients. to our knowledge this is the first report of bacteriologically proven cases of brucellar epididymoor ... | 2001 | 17664835 |
| actual antibiotic resistance pattern of brucella melitensis in central anatolia. an update from an endemic region. | to test in vitro susceptibilities of brucella melitensis (b. melitensis) blood isolates obtained from an endemic region, by broth microdilution susceptibility test. | 2007 | 17676210 |
| characterisation of human outbreaks of brucellosis and sporadic cases by the use of hyper-variable octameric oligonucleotide fingerprint (hoof) variable number tandem repeats. | hyper-variable octameric oligonucleotide fingerprints (hoofs) enable typing of brucella spp. by targeting the 8-bp tandem repeat in eight loci that vary in number (variable number tandem repeats; vntrs). brucella is one of the most important zoonotic pathogens, because of its public health and economic consequences. to assess the role of hoofs as epidemiological markers for brucella melitensis, which is the main species involved in human brucellosis in spain, 87 sporadic and outbreak isolates we ... | 2007 | 17686139 |
| partial protection against brucella infection in mice by immunization with nonpathogenic alphaproteobacteria. | previous findings indicate that brucella antigens and those from nonpathogenic alphaproteobacteria (npap) are cross-recognized by the immune system. we hypothesized that immunization with npap would protect mice from brucella infection. mice were immunized subcutaneously with heat-killed ochrobactrum anthropi, sinorhizobium meliloti, mesorhizobium loti, agrobacterium tumefaciens, or brucella melitensis h38 (standard positive control) before intravenous challenge with brucella abortus 2308. cross ... | 2007 | 17715332 |
| blue-light-activated histidine kinases: two-component sensors in bacteria. | histidine kinases, used for environmental sensing by bacterial two-component systems, are involved in regulation of bacterial gene expression, chemotaxis, phototaxis, and virulence. flavin-containing domains function as light-sensory modules in plant and algal phototropins and in fungal blue-light receptors. we have discovered that the prokaryotes brucella melitensis, brucella abortus, erythrobacter litoralis, and pseudomonas syringae contain light-activated histidine kinases that bind a flavin ... | 2007 | 17717187 |
| toxic-febrile neurobrucellosis, clinical findings and outcome of treatment of four cases based on our experience. | central nervous system (cns) involvement is uncommon in brucellosis. neurological complications of brucellosis may be divided into 2 major groups: 1) those related to the acute-febrile state that occurs in acute disease (toxic-febrile neurobrucellosis), and 2) those related to actual invasion and localization of the pathogen in the cns (classical neurobrucellosis). in this paper, we present 4 cases of toxic-febrile neurobrucellosis and discuss clinical findings and outcome of treatment in these ... | 2007 | 17852920 |
| brucella peritonitis and leucocytoclastic vasculitis due to brucella melitensis. | brucellosis is a multisystemic disease that rarely leads to a fatal outcome. while reticuloendothelial system organs are mostly affected, peritonitis and posthepatitic cirrhosis are also complications of brucellosis, though they are very rare. brucella spp. can also trigger immunological reactions. we report a case of brucellosis with peritonitis, renal failure and leucocytoclastic vasculitis caused by brucella melitensis, which led to a fatal outcome. brucellosis should be considered in the dif ... | 2007 | 17874004 |
| the bmei0216 gene of brucella melitensis is required for internalization in hela cells. | brucella is an intracellular facultative bacterium able to survive and multiply in professional and non-professional phagocytes. however, its adhesion and invasion mechanisms have not been elucidated yet. in this work, we assess the interruption of a bmei0216 gene of brucella melitensis, by using hela epithelial cells and murine macrophages for invasion and replication assays. the mutation did not affect survival or multiplication within macrophages. likewise, invasion assays with hela cells rev ... | 2008 | 17881185 |
| brucellosis in a returned traveler and his wife: probable person-to-person transmission of brucella melitensis. | 2007 | 17883467 | |
| an aerosolized brucella spp. challenge model for laboratory animals. | to characterize the optimal aerosol dosage of brucella abortus strain 2308 (s2308) and b. melitensis (s16m) in a laboratory animal model of brucellosis, dosages of 10(3)-10(10) colony forming units (cfu) were nebulized to mice. although tissue weights were minimally influenced, total cfu per tissues increased beginning at 10(6)-10(7) cfu dosages, with 10(9) cfu appearing to be an optimal dosage for s16m or s2308 aerosol delivery. at 12 weeks after vaccination with 10(7) cfu of b. abortus strain ... | 2007 | 17894637 |
| review of clinical and laboratory features of human brucellosis. | infection with brucella spp. continues to pose a human health risk globally despite strides in eradicating the disease from domestic animals. brucellosis has been an emerging disease since the discovery of brucella melitensis by sir david bruce in 1887. although many countries have eradicated b. abortus from cattle, in some areas b. melitensis and b. suis have emerged as causes of this infection in cattle, leading to human infections. currently b. melitensis remains the principal cause of human ... | 2007 | 17901634 |
| uptake of ciprofloxacin and ofloxacin by 2 brucella strains and their fluoroquinolone-resistant variants under different conditions. an in vitro study. | the existence of an efflux-mediated mechanism involved in the drug accumulation process of the 2 resistant brucella strains was investigated; 2 susceptible brucella strains, brucella melitensis biovar abortus 2308 (s2308) and afbortus 3196 (s3196) strains, were the reference strains cultured on trypticase soy agar (tsa) medium and incubated to gradient concentrations of ciprofloxacin and ofloxacin antibiotics. the uptake level of 2 antibacterial agents in 2 susceptible and their fluoroquinolones ... | 2007 | 17920802 |
| myocardial noncompaction presenting with brucella endocarditis. | noncompaction of ventricular myocardium is a unique congenital cardiomyopathy with its own clinical presentation and course. it is more frequently associated with complications of congestive heart failure, thromboembolism and malignant ventricular arrhythmias; however, concomitance of valvular pathologies or infective endocarditis with noncompaction of ventricular myocardium has seldom been reported. we describe herein the first case of left ventricular myocardial noncompaction presenting with b ... | 2009 | 17931725 |
| [isolation of brucella melitensis from ejaculate culture of a brucellosis patient with epididymoorchitis]. | brucellosis is a significant public health problem particularly in developing countries. people are frequently infected through milk, milk products, urine and pregnancy material of animals with brucellosis. epididymoorchitis is the most frequent genitourinary complication of brucellosis and is often unilateral. in this report, a 35 years old male patient who was diagnosed as epididymoorchitis based on clinical presentation, laboratory findings and imaging techniques, has been presented. brucella ... | 2007 | 17933260 |
| cytotoxicity in macrophages infected with rough brucella mutants is type iv secretion system dependent. | smooth brucella spp. inhibit macrophage apoptosis, whereas rough brucella mutants induce macrophage oncotic and necrotic cell death. however, the mechanisms and genes responsible for brucella cytotoxicity have not been identified. in the current study, a random mutagenesis approach was used to create a mutant bank consisting of 11,354 mutants by mariner transposon mutagenesis using brucella melitensis rough mutant 16m delta manba as the parental strain. subsequent screening identified 56 mutants ... | 2008 | 17938217 |
| brucellosis of the lung: case report and review of the literature. | human brucellosis is a worldwide re-emerging zoonosis. however, its histological appearance has only been occasionally described. we report the case of a young girl who had been suffering from a spontaneous fracture of the eighth thoracic vertebra at the age of 7. at the age of 15, x-ray showed a translucence of the seventh and ninth thoracic vertebra, and additionally, a bi-lateral episcleritis was detected. three months later, she was admitted to the hospital because of perspiration at night a ... | 2008 | 17952458 |
| comparison of seven commercial dna extraction kits for the recovery of brucella dna from spiked human serum samples using real-time pcr. | we compared the relative recovery of extraction of bacterial dna from serum using seven commercial kits (ultraclean dna bloodspin kit, puregene dna purification system, wizard genomic dna purification kit, high pure pcr template preparation kit, gfx genomic blood dna purification kit, nucleospin tissue kit, and qiaamp dna blood mini kit). human serum samples were spiked with known concentrations of brucella melitensis rev 1; the dna was extracted and tested in genus-specific lightcycler polymera ... | 2008 | 17973130 |
| rapid diagnosis of human brucellosis by quantitative real-time pcr: a case report of brucellar spondylitis. | blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time pcr to detect brucella melitensis. the high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods. | 2008 | 17977995 |
| a brucellosis case presenting with mass formation suggestive for tumor in soft tissue. | we report here a 70-year-old female patient who was diagnosed with brucellosis and presented with mass formation resembling a tumor. the mass was protuberant, 10 cm from the skin surface with a diameter of 15 cm, located at the inferior-lateral region of the left scapula. brucella melitensis was yielded from culture of mass fluid. the patient responded to ceftriaxone, rifampin and doxycycline therapy and recovered without any sequela at the end of surgery and 3 months of medical treatment. | 2007 | 17984747 |
| water-borne outbreak of brucella melitensis infection. | 1939 | 18015070 | |
| a study of an epidemic of brucellosis due to brucella melitensis. | 1940 | 18015286 | |
| bacteremia is as unpredictable as clinical manifestations in human brucellosis. | because of the suboptimal recovery rate of brucellae from blood, it has been proposed that cultures of bone marrow, liver tissue, and lymph nodes may improve the recovery rate of the organism. data in support of these recommendations are limited and not clearly convincing, especially that of bone marrow culture. the main purpose of this work was to evaluate the roles of blood, bone marrow, liver, and lymph node cultures in the diagnosis of human brucellosis. | 2008 | 18023384 |
| brucella bacteremia in patients with acute leukemia: a case series. | abstract: | 2007 | 18036218 |
| specific detection and differentiation of ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. by a multi-primer pcr that targets the reca gene. | ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. consequently, difficulties in their identification and differentiation have been reported. in this study, a sensitive reca gene-based multi-primer single-target pcr (mp-st-pcr) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. the specificity of the assa ... | 2008 | 18065669 |
| mutant prevention concentrations of tetracycline, rifampicin and ciprofloxacin for brucellae melitensis. | 2007 | 18073161 | |
| virb12 is a serological marker of brucella infection in experimental and natural hosts. | the brucella species type iv secretion system, encoded by the virb1-12 locus, is required for intracellular replication and persistent infection in vivo. the requirement of virb proteins for infection suggests that they are expressed in vivo and may therefore represent serological markers of infection. to test this idea, we purified recombinant virb1, virb5, virb11, and virb12 and tested for their recognition by antibodies in sera from experimentally infected mice and goats by using an indirect ... | 2008 | 18077620 |
| splenic puncture: diagnostic accuracy and safety in infectious diseases. | 2008 | 18082440 | |
| aureomycin therapy in human brucellosis due to brucella melitensis. | 1948 | 18105995 | |
| design and implementation of a database for brucella melitensis genome annotation. | the genome sequences of three brucella biovars and of some species close to brucella sp. have become available, leading to new relationship analysis. moreover, the automatic genome annotation of the pathogenic bacteria brucella melitensis has been manually corrected by a consortium of experts, leading to 899 modifications of start sites predictions among the 3198 open reading frames (orfs) examined. this new annotation, coupled with the results of automatic annotation tools of the complete genom ... | 2008 | 18160234 |
| [evaluation of diagnostic value of brucellacapt test in brucellosis]. | although standard tube agglutination (sta) test is the most preferred method for the serodiagnosis of brucellosis, it may yield false negative results because of its incompetence in detecting the blocking (non-agglutinating) antibodies. blocking antibodies can be detected by coombs' test (anti-human globulin test), which is generally not performed in laboratories routinely since it is a time consuming and laborious method. recently more practical brucellacapt (immunocapture-agglutination) test i ... | 2007 | 18173079 |
| brucellosis in cases of end-stage renal disease. | patients with brucellosis frequently present with joint and bone pain. however, brucellosis may be overlooked in patients with end-stage renal disease (esrd) who undergo dialysis since amyloidosis due to renal osteodystrophy and beta-2 microglobulinaemia may cause bone pain as well. only four cases of end-stage renal failure accompanied by brucellosis have been reported in the literature. we evaluated clinical and laboratory characteristics and organ involvement of seven brucellosis patients wit ... | 2008 | 18178600 |
| comparison of culture and pcr for the detection of brucella melitensis in blood and lymphoid tissues of serologically positive and negative slaughtered sheep. | to compare the culture and pcr methods for detection of brucella melitensis in blood and lymphoid tissue samples obtained from slaughtered sheep (n = 162) testing positive/negative in serological tests (rose bengal test and serum agglutination test). | 2008 | 18179446 |
| comparison of pcr assay and bacteriological culture method for the detection of brucella melitensis in stomach content samples of aborted sheep fetuses. | the aim of this study was to evaluate the polymerase chain reaction (pcr) assay for detection of brucella melitensis in stomach content samples of aborted sheep fetuses and to compare its performance with bacteriological culture method. it was also aimed to determine the agreement between pcr and rose bengal plate test (rbpt). materials were collected from aborted sheep from 109 different sheep flocks in the region of van during the lambing seasons of 2004-2005 and 2005-2006. stomach contents fr ... | 2007 | 18181360 |
| the many faces of human-to-human transmission of brucellosis: congenital infection and outbreak of nosocomial disease related to an unrecognized clinical case. | because person-to-person transmission of brucellosis is exceptional, physicians who care for patients with this disease are not considered to be at increased risk. a woman in her 24th week of pregnancy who had received a diagnosis of placenta previa presented to the hospital with massive vaginal bleeding and hypovolemic shock, requiring performance of an emergency cesarean delivery. two physicians who assisted the surgical delivery developed culture-proven brucella melitensis infection. the orga ... | 2007 | 18190307 |
| brucella glomerulonephritis resulting in end-stage renal disease: a case report and a brief review of the literature. | brucella glomerulonephritis is a rare condition with only a few reported cases. we review the literature, and describe a 24-year-old female who presented with edema and proteinuria. blood grew brucella melitensis. renal biopsy showed diffuse proliferative glomerulonephritis. the patient progressed to end-stage renal disease despite antibiotic and steroid therapy. | 2008 | 18196469 |
| non-imported brucellosis outbreak from unpasteurized raw milk in moroccan immigrants in spain. | nine cases of brucellosis were identified in the city of elche (comunidad valenciana, spain) in two families of moroccan immigrants. all of the patients had drunk unpasteurized raw milk from goats. brucella melitensis biovar 3 was identified in clinical specimens. preventive measures for brucellosis should be implemented among immigrant populations in spain. | 2008 | 18205974 |
| an unusual presentation of brucellar septic arthritis involving the knee joint with extraarticular hardware: a case report. | brucellosis is a zoonotic infection with a wide spectrum of clinical presentations. we present a rare case of brucellosis with osteoarticular involvement complicated by a femoral intramedullary nail protruding the suprapatellar pouch. although brucella melitensis is a rare cause of brucellar septic arthritis, it should not be excluded from the list of suspected organisms in endemic areas for the disease. | 2008 | 18207411 |
| brucellar spondylodiscitis: case report. | brucellosis is a common zoonosis which still remains a major health problem in certain parts of the world. osteoarticular involvement is the most frequent complication of brucellosis, in which the diagnosis of brucellar spondylodiscitis is often difficult since the clinical presentation may be obscured by many other conditions. herein, we report an uncommon case of spondylodiscitis due to brucella in a male who presented with abdominal pain. the diagnosis was established by positron emission tom ... | 2007 | 18229463 |
| brucellar arthritis of the knee: a case report with delayed diagnosis. | brucellar septic arthritis has become extremely rare in western developed countries since bovine brucellosis has been successfully eradicated. nevertheless, we should remain vigilant for this disease, as brucellosis is still endemic in many parts of the world. the authors present the rare manifestation of a brucellar septic arthritis of the knee joint, associated with a lytic lesion of the proximal tibia. the diagnosis of brucellosis was delayed by 11 months because of the microbiological and hi ... | 2007 | 18260497 |
| identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis. | vitamin b(12), the antipernicious anemia factor, is the cyano derivative of adenosylcobalamin, which is one of nature's most complex coenzymes. adenosylcobalamin is made along one of two similar yet distinct metabolic pathways, which are referred to as the aerobic and anaerobic routes. the aerobic pathway for cobalamin biosynthesis proceeds via cobalt insertion into a ring-contracted macrocycle, which is closely followed by adenosylation of the cobalt ion. an important prerequisite for adenosyla ... | 2008 | 18263579 |
| use of mlva-16 typing to trace the source of a laboratory-acquired brucella infection. | 2008 | 18289724 | |
| brucellar meningitis in an infant--evidence for human breast milk transmission. | brucellar meningitis is relatively uncommon, especially in children younger than 1 year. we present another case of meningitis due to brucella melitensis biotype 1 in a 11-month-old infant transmitted by breast milk. this is the first report of successful isolation of b. melitensis from the breast milk in the literature. babies of infected nursing mother should be monitored closely for evidence of infection since the breast milk is the source of transmission of brucellosis. it would be prudent t ... | 2008 | 18292100 |
| putative quorum-sensing regulator blxr of brucella melitensis regulates virulence factors including the type iv secretion system and flagella. | brucella melitensis is an intracellular pathogen that establishes a replicative niche within macrophages. while the intracellular lifestyle of brucella is poorly understood and few virulence factors have been identified, components of a quorum-sensing pathway in brucella have recently been identified. the luxr-type regulatory protein, vjbr, and an n-acylhomoserine lactone signaling molecule are both involved in regulating expression of the virb-encoded type iv secretion system. we have identifie ... | 2008 | 18310341 |
| subversion of toll-like receptor signaling by a unique family of bacterial toll/interleukin-1 receptor domain-containing proteins. | pathogenic microbes have evolved sophisticated molecular strategies to subvert host defenses. here we show that virulent bacteria interfere directly with toll-like receptor (tlr) function by secreting inhibitory homologs of the toll/interleukin-1 receptor (tir) domain. genes encoding tir domain containing-proteins (tcps) were identified in escherichia coli cft073 (tcpc) and brucella melitensis (tcpb). we found that tcpc is common in the most virulent uropathogenic e. coli strains and promotes ba ... | 2008 | 18327267 |
| unusual manifestation of brucellosis: liver abscess and pancytopenia caused by brucella melitensis. | brucellosis is a zoonosis with a worldwide distribution, and still endemic in greece. although hepatic involvement is common, cholecystitis and hepatic abscesses represent rare complications of the disease. hematological manifestations of brucellosis include various abnormalities such as anemia and leucopenia, but the disease rarely presents with pancytopenia. we present a case of liver abscess and pancytopenia caused by brucella melitensis in a 54-year-old worker who consumed unpasteurized dair ... | 2008 | 18334880 |
| brucellosis in camels (camelus dromedarius) in darfur, western sudan. | in a field outbreak of brucellosis in 21 camels mixed with cattle, sheep and goats, five camels, three of which showed clinical signs, were serologically positive. in a subsequent abattoir survey of apparently healthy camels, six animals were seropositive, albeit with titres that tended to be lower than those found in the field outbreak. of the six seropositive slaughtered camels, five were shown to have lymph nodes (prescapular and supramammary) infected with brucellae (brucella melitensis biov ... | 2008 | 18346482 |
| brucella melitensis b115-based complement fixation test to detect antibodies induced by brucella rough strains. | to assess the efficiency of a brucella melitensis b115 rough strain, naturally devoid of anticomplementary activity, used as antigen in a complement fixation test (cft) to detect antibodies induced by brucella strains with rough phenotype, such as brucella abortus rb51, brucella ovis and brucella canis. | 2008 | 18355234 |
| improved performance of brucella melitensis native hapten over brucella abortus ops tracer on goat antibody detection by the fluorescence polarization assay. | the current method for goat brucellosis diagnosis is based on the world organization for animal health (oie) using the screening card test (ct), with antigen at 8% (ct8) or 3% (ct3) of cell concentrations, and the confirmatory complement fixation test (cft). however, these tests do not differentiate antibodies induced by vaccination from those derived from field infections by brucella species or other bacterial agents; in places like mexico, where the prevalence of brucellosis and the vaccinatio ... | 2008 | 18359093 |
| panniculitis as the initial manifestation of brucellosis: a case report. | here, we describe a 38-year-old male with an abrupt manifestation acute panniculitis as unusual presentation of brucellosis. brucellosis is a reemerging disease in turkey, and the disease is primarily transmitted from farm animals to humans. farmers and shepherds are the major risk groups for brucellosis in anatolia. brucellosis may involve almost all systems and organs, including the skin, and may mimic a wide range of illness and syndromes. although the cutaneous manifestation of brucellosis i ... | 2008 | 18360123 |
| immunization with a single dose of a microencapsulated brucella melitensis mutant enhances protection against wild-type challenge. | the development of safe and efficacious immunization systems to prevent brucellosis is needed to overcome the disadvantages of the currently licensed vaccine strains that restrict their use in humans. alginate microspheres coated with a protein of the parasite fasciola hepatica (vitelline protein b [vpb]) and containing live brucella melitensis attenuated mutant vjbr::tn5 (bmeii1116) were evaluated for vaccine efficacy and immunogenicity in mice. a single immunization dose in balb/c mice with th ... | 2008 | 18362129 |
| brucella infection in total knee arthroplasty. case report and revision of the literature. | brucella infection in arthroplasties is a rare event. an unspecific clinical symptomatology is associated with unclear radiographic peri-prosthetic signs of bone halisteresis. only a positive anamnesis, combined with an antibiogram of the joint liquid and a high serum antibody titre, can lead to a definitive diagnosis. we report a case of brucella melitensis infection in a total knee arthroplasty implanted 2 years earlier. with the absence of radiological signs of prosthetic loosening and thanks ... | 2008 | 18369535 |
| [consider brucellosis if the patient suffers of protracted fever! visiting middle east a high-risk factor]. | 2008 | 18376709 | |
| use of the brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay. | the performance of the fluorescence polarization assay (fpa) using the recently described brucella melitensis native hapten and the brucella abortus o-polysaccharide tracer was evaluated and compared with those of the world organization for animal health tests related to indirect and competitive enzyme-linked immunosorbent assays as classification variables for goat sera obtained from a high-prevalence area where vaccination was performed; test series were also evaluated to increase the final sp ... | 2008 | 18385457 |
| [clinical manifestations, complications and treatment of brucellosis: 45-patient study]. | the aim of this study was to evaluate the clinical, laboratory findings and therapeutic features of patients with brucellosis. the diagnosis was made by clinical findings, automated blood culture, serology (rose bengal plate agglutination test, standard tube agglutination (wright) and immunofluorerescence). the susceptibility of 13 strains was tested in vitro. the base sequence was determined for four strains. forty-five cases were collected (31 acute and 14 sub-acute). contamination was digesti ... | 2009 | 18387752 |
| in vitro activity of tigecycline, tetracycline and fluoroquinolones against brucella melitensis. | brucellosis is a major zoonotic disease of global importance. forty years after active drug therapy was defined, there is no optimum antibiotic therapy for brucellosis due to relatively high rates of relapse and treatment failure. the aim of this study was to assess the in vitro susceptibility of 60 brucella melitensis isolates obtained from blood and fluids to tigecycline, tetracycline and three quinolones (ciprofloxacin, levofloxacin, moxifloxacin). the minimum inhibitory concentrations (mics) ... | 2008 | 18390405 |
| severe pharyngitis in stockbreeders: an unusual presentation of brucellosis. | abstract brucellosis is a known occupational hazard for shepherds, abattoir workers, veterinarians, dairy industry professionals and personnel in microbiological laboratories. any organ may be affected by brucella species but to date, severe manifestations in the pharynx have never been reported as the prevailing features of brucellosis. we report two cases in stockbreeders who presented with high-grade fever and severe exudative pharyngitis accompanied by severe odynophagia in the first and wit ... | 2008 | 18397911 |
| a study on the use of male animal models for developing a live vaccine for brucellosis. | to study the safety of brucella melitensis wr201, a live vaccine candidate, we compared the course of infection of this strain with that of virulent 16m in male balb/c mice. at various times after oral immunization with strains wr201 or 16m, lungs, liver, spleen, testis, epididymis, inguinal and cervical lymph nodes were removed. tissues were divided for microbiologic culture and histopathological examination. wr201 infection in male balb/c mice had lower intensity and shorter duration than infe ... | 2008 | 18405337 |
| [ticks bite in foresters]. | the objective of the study is evalutation of the risk for ticks strings on foresters. the sample constituted by 325 foresters belong to messina province as been submitted to medical examination venous tests. whole sample had to answer to a questionnaire to consider. the prevalence of systemic and skin reactions and we have dose immunoglobulines versus brucella melitensis, rickettsie conorii e borrelia burgdorferi. the results showed that the 19% has declared past stings of tick, and 4.9% reporte ... | 2007 | 18409975 |
| ocular manifestations associated with brucellosis: a 26-year experience in peru. | brucellosis has unusual clinical manifestations. ocular involvement caused by brucellosis remains poorly recognized in areas in which brucellosis is endemic. | 2008 | 18419433 |
| immunopathological responses and kinetics of brucella melitensis rev 1 infection after subcutaneous or conjunctival vaccination in rams. | the innocuousness of the brucella melitensis rev 1 live attenuated vaccine strain has never been fully assessed in rams. the immunopathological responses and the kinetics and distribution of the infection induced by this strain were determined after subcutaneous or conjunctival vaccination in both young (3-4 months old) and adult (12 months old) rams. at regular intervals after vaccination the animals were bled for serological studies, and slaughtered for both pathological and bacteriological ex ... | 2008 | 18423950 |
| ultraviolet c lethal effect on brucella melitensis. | the gram-negative bacteria brucella melitensis was investigated to evaluate its susceptibility to uvc radiation at 254 nm. at an intensity of 18.7 mw/cm2 of uvc, the time required for inactivation of b. melitensis was 240 seconds in both dark and light, whereas it was 120 seconds and 240 seconds in dark and light respectively at an intensity of 19.5 mw/cm2. the results indicate that vaccinal strain of b. melitensis (rev.1) is more sensitive to uvc than wild b. melitensis strain. | 2008 | 18437841 |
| [short communication: investigation of in vitro antibiotic susceptibility of brucella melitensis]. | the aim of this study was to investigate the in vitro susceptibilities of brucella melitensis clinical isolates against antibiotics used in conventional treatment regimens and additionally against quinolones. a total of 65 b. melitensis strains isolated from blood and bone marrow specimens of patients who were admitted to an university hospital localized in a region endemic for brucellosis. minimum inhibitory concentration values (mic50 and mic90) of trimethoprim-sulphamethoxazole (tmp-smx), rif ... | 2008 | 18444570 |
| diagnostic and therapeutic challenges of childhood brucellosis in a nonendemic country. | we sought [corrected] to define the current epidemiology, clinical manifestations, and course of childhood brucellosis in the united states. | 2008 | 18450861 |
| an eternal microbe: brucella dna load persists for years after clinical cure. | despite the continuing high incidence of brucellosis, vague aspects of pathophysiology, diagnosis, and treatment continue to exist, particularly with regard to the ability of brucella species to survive inside the host. | 2008 | 18462106 |
| risk factors associated with camel brucellosis in jordan. | during the period between february, 2004 and december, 2006, a cross-sectional study was performed to investigate some epidemiological aspects related to camel brucellosis in jordan. four hundred twelve camel sera from 37 herds were randomly collected and analyzed using rose bengal plate test and complement fixation test. a structured pre-tested questionnaire was administered to collect information on camel herd health and management. a multivariable logistic regression model was constructed to ... | 2008 | 18484121 |
| cold agglutinin-associated hemolytic anemia due to brucellosis: first case report. | 2008 | 18512765 | |
| intracellular rescuing of a b. melitensis 16m virb mutant by co-infection with a wild type strain. | brucella is a broad-range, facultative intracellular pathogen that can survive and replicate in an endoplasmic reticulum (er)-derived replication niche by preventing fusion of its membrane-bound compartment with late endosomes and lysosomes. this vacuolar hijacking was demonstrated to be dependent on the type iv secretion system virb but no secreted effectors have been identified yet. a virb mutant is unable to reach its er-derived replicative niche and does not multiply intracellularly. in this ... | 2008 | 18547782 |
| [laboratory-acquired brucellosis]. | brucellosis is a rare disease in denmark. we describe one case of laboratory-acquired brucellosis from an index patient to a laboratory technician following exposure to an infected blood culture in a clinical microbiology laboratory. | 2008 | 18565306 |
| in vitro-selected resistance to fluoroquinolones in two brucella strains associated with mutational changes in gyra. | in the present study, brucella melitensis biovar abortus 2308 and brucella abortus 3196 biotype 5 reference strains, which are susceptible to fluoroquinolones, became in vitro-resistant to fluoroquinolones by culture in trypticase soy agar. the quinolone resistance-determining regions (qrdrs) of the gyra and parc genes of the two reference strains were analysed by polymerase chain reaction sequencing analysis to obtain the wild-type sequence. these sequences were then compared with the correspon ... | 2008 | 18583100 |
| [response to the treatment of brucellosis among children. evaluation with huddleson reaction and pcr]. | brucellosis poses a significant public health problem and requires meticulous diagnosis; the outcome has frequent relapses even when the treatment is appropriate. | 2007 | 18593545 |
| genomic island 2 of brucella melitensis is a major virulence determinant: functional analyses of genomic islands. | brucella genomic islands (gis) share similarities in their genomic organization to pathogenicity islands from other bacteria and are likely acquired by lateral gene transfer. here, we report the identification of a gi that is important for the pathogenicity of brucella melitensis. the deletion of gi-1, gi-5, or gi-6 did not affect bacterial growth in macrophages as well as their virulence in interferon regulatory factor 1-deficient (irf-1(-/-)) mice, suggesting that these islands do not contribu ... | 2008 | 18641138 |
| brucellosis vaccines: assessment of brucella melitensis lipopolysaccharide rough mutants defective in core and o-polysaccharide synthesis and export. | the brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. in endemic areas, vaccination is the only effective way to control this disease. brucella melitensis rev 1 is a vaccine effective against the brucellosis of sheep and goat caused by b. melitensis, the commonest source of human infection. however, rev 1 carries a smooth lipopolysaccharide with an o-polysaccharide that elicits antibodies interfering in serodiagnosis, a major problem in ... | 2008 | 18648644 |
| brucella: a mr "hide" converted into dr jekyll. | dr david bruce (1855-1931) first identified the causative agent of brucellosis as a small gram-negative alpha-proteobacterium, which was later on called brucella melitensis in his honor by meyer and shaw. nowadays, four strains exhibit pathogenicity in humans with b. melitensis being the least host specific and also the most infectious for humans. the other strains are brucella suis and brucella abortus and more recently human cases being infected with brucella cetaceae have been reported. why s ... | 2008 | 18664389 |
| novel identification and differentiation of brucella melitensis, b. abortus, b. suis, b. ovis, b. canis, and b. neotomae suitable for both conventional and real-time pcr systems. | we describe the development of a novel pcr assay for the rapid detection of members of the brucella genus, and the differentiation between six recognized brucella species. the assay has proven to be highly specific with the additional advantage of being suitable for use with both conventional and real-time pcr. | 2008 | 18675856 |
| evaluation of brucella melitensis b115 as rough-phenotype vaccine against b. melitensis and b. ovis infections. | brucella melitensis strain rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. because of its smooth phenotype, however, it induces antibodies directed to the o-polysaccharide (o-ps) of the lipopolysaccharide (lps), thus unabling to distinguish between vaccinated and infected animals. it has been speculated that alternative vaccines could be live, attenuated brucella rough strains, which are devoid of the o-ps. b. melitensis b115 is a natural, attenuated, rough strain. ... | 2008 | 18675869 |
| rifampicin resistance phenotyping of brucella melitensis by rpob gene analysis in clinical isolates. | r rifampicin resistance of brucella melitensis by rpob gene analysis has not yet been performed in turkey, where brucellosis is endemic. in this study, we investigated the efficacy of e-test and single nucleotide polymorphism (snp) analysis of the b. melitensis rpob gene, for the detection of mutations conferring rifampicin resistance, by sequencing 21 human b. melitensis strains from the southeast and marmara regions of turkey. on clsi slow-growing bacteria standards, all isolates were sensitiv ... | 2008 | 18676221 |
| acute infection by conjunctival route with brucella melitensis induces igg+ cells and ifn-gamma producing cells in peripheral and mucosal lymph nodes in sheep. | the early distribution of brucella melitensis and the immune response induced in lymphoid tissues and lymph nodes (ln) draining the upper respiratory tract were analysed in sheep. an experimental acute infection was performed by inoculating the sheep with the virulent h38 strain of b. melitensis by the conjunctival route. the infection was rapidly controlled at the site of inoculation but resulted in a local and systemic dissemination of brucellae mainly in the pharyngeal tonsil, local and perip ... | 2008 | 18761416 |
| the putative penicillin-binding proteins 1 and 2 are important for viability, growth and cell morphology of brucella melitensis. | the penicillin-binding proteins (pbps) are enzymes that regulate the assembly of the peptidoglycan layer of the bacterial cell wall. the genome of brucella melitensis strain 16m possesses seven pbp genes: three in pbp-1 family (designated as 1a, 1b, and 1c); one in pbp-2 family; and three in pbp-6 family (designated as 6a, 6b, and 6c). we investigated the importance of pbp-1 and pbp-2 genes to viability, cell morphology and infectivity of b. melitensis. a recombinant b. melitensis strain (design ... | 2009 | 18809265 |
| comparison of heat shock response in brucella abortus and brucella melitensis. | heat shock protein (hsp) is highly conserved, that serves a wide range of function in protein folding and transport. it protect from various type of stress including heat shocks. however, it is well known that the virulence of b. melitensis is more than b. abortus, but there is not any strong evidence to verify it. for this purpose, in refer to potent antigenicity of hsps in various infectious as well as some hsp molecules act as potent activator of macrophage (danger signal), we hypothesized th ... | 2008 | 18817188 |
| antibacterial activity of the fruits of iranian torilis leptophylla against some clinical pathogens. | the aim of this study was to examine the antimicrobial activity of the methanolic extract of torilis leptophylla was tested on eleven bacteria (bacillus anthracis, bacillus subtilis, bacillus pumilus, staphylococcus aureus, bacillus licheniformis, brucella melitensis, escherichia coli, salmonella typhi, proteus mirabilis, bordetella bronshiseptica and pseudomonas aeruginosa). tested extract was effective against all bacteria but not b. subtilis. consequently, the ethanolic extract had antibacter ... | 2008 | 18819541 |
| [brucellosis spondilitis in a ten year boy]. | brucellosis is acute infectious disease classified as zoonosis, which can leave humans with chronic damages and disability. it can appear as sporadic cases or a smaller epidemic anywhere in the world but it is more common in some areas and there it presents significant public health issue. clinical picture is very variegated. a long period of latency is possible, unnoticed asymptomatic start or forms with mild clinical picture up to the forms with per acute septic-toxic shock and possible lethal ... | 2008 | 18822949 |
| rare red rash. | 2008 | 18823855 | |
| evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human brucella isolates in a region of brucellosis endemicity. | brucellosis remains an important anthropozoonosis worldwide. brucella species are genetically homogeneous, and thus, the typing of brucella species for epidemiological purposes by conventional molecular typing methods has remained elusive. although many methods could segregate isolates into the phylogenetically recognized taxa, limited within-species genetic diversity has been identified. recently, multilocus variable-number tandem-repeat analysis (mlva) was found to have a high degree of resolu ... | 2008 | 18923007 |
| repeatability and pattern recognition of bacterial fatty acid profiles generated by direct mass spectrometric analysis of in situ thermal hydrolysis/methylation of whole cells. | direct ci mass spectrometry profiling of fatty acid methyl esters (fames) from in situ thermal hydrolysis/methylation (thm) of whole bacterial cells with tetramethylammonium hydroxide (tmah) has been demonstrated as a potential method for real time and fieldable detection/identification of microorganisms. bacillus anthracis (ames), yersinia pestis (nair. kenya), vibrio cholerae (e1 tor), brucella melitensis (abortus wild) and francisella tularensis (lvs vaccine) were profiled by this method duri ... | 2003 | 18968943 |
| case records of the massachusetts general hospital. case 34-2008. a 58-year-old woman with neck pain and fever. | 2008 | 18971496 | |
| evaluation of different serological techniques in laboratory diagnosis of brucellosis. | diagnosis of brucellosis is vital for early institution of proper therapy as untreated cases may progress to chronic stage. though the demonstration of the causative agent in blood is considered as the most conclusive test in the diagnosis of brucellosis, isolation of brucella organism by blood culture is relatively low. hence a number of sensitive and rapid serological tests have been introduced for the diagnosis of brucellosis. in the present study, an attempt was made to compare the efficacie ... | 2008 | 18975512 |
| [a rare complication of brucellosis: mitral valve endocarditis]. | early diagnosis of brucella endocarditis is of paramount importance because of its fatal consequences. the most commonly affected localization is the aortic valve, while mitral valve involvement is rare. a 44-year-old male patient with a history of rheumatic heart disease presented with fever, fatigue, and back pain. three consecutive blood cultures revealed growth of brucella melitensis. on transthoracic echocardiography, mitral valve area was 1.5 cm2 and there was mild mitral regurgitation. tr ... | 2008 | 18984985 |
| immunohistochemical detection of brucella melitensis antigens in cases of naturally occurring abortions in sheep. | brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. the present study was carried out to determine, by immunohistochemistry, the presence of b. melitensis antigens in 110 naturally occurring aborted sheep fetuses. sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect brucella antigens. brucella melitensis antigens were detected in 33 of 110 fetuses (30%). in the 33 positive c ... | 2008 | 18987234 |