Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| mimotope identification from monoclonal antibodies of burkholderia pseudomallei using random peptide phage libraries. | this study used random peptide libraries, displayed by bacteriophage t7 and m13, to identify mimotopes from four monoclonal antibodies (mabs) specific to burkholderia pseudomallei. bound phages, selected from fourth-round panning with each mab, were tested for binding specificity with each mab using elisa, before being further amplified and checked for phage peptide sequence using pcr and dna sequencing. overall, 75 of 90 phages (83.3%) were elisa-positive with each mab. mimotopes from all 75 ph ... | 2008 | 19121687 |
| soil physicochemical properties related to the presence of burkholderia pseudomallei. | the incidence of burkholderia pseudomallei in the soil from north-east thailand is estimated to be 20-fold higher than that from central thailand, and is associated with a 10-fold higher incidence of melioidosis in the region than in central thailand. this study investigated the presence of b. pseudomallei in relation to the physicochemical properties of soil from khon kaen province, north-east thailand. thirteen districts (54.2%) were positive for b. pseudomallei. from a selected district, b. p ... | 2008 | 19121688 |
| development of aptamers specific for potential diagnostic targets in burkholderia pseudomallei. | improved diagnostic reagents would be of considerable benefit in enhancing the specificity and sensitivity of rapid assays for burkholderia pseudomallei, the causative agent of melioidosis. the purpose of this work is to develop aptamers, high affinity rna-based molecular recognition molecules, which could be used as reagents for identification of the whole organism in assays of biological samples. data are presented demonstrating the purification of recombinant b. pseudomallei secreted or surfa ... | 2008 | 19121689 |
| in silico analysis of potential diagnostic targets from burkholderia pseudomallei. | administration of appropriate therapeutic regimes for infections arising from pathogenic species of burkholderia is critically dependent upon rapid and accurate diagnoses. the purpose of this work is to establish a bioinformatic pipeline to assess protein sequences for their potential as diagnostic targets for the detection of burkholderia species. data are presented showing both a bioinformatic methodology for prediction of surface-associated and secreted proteins and its application to a test ... | 2008 | 19121691 |
| seropositivity to burkholderia pseudomallei does not reflect the development of cell-mediated immunity. | cell-mediated immunity to burkholderia pseudomallei, the causative agent of melioidosis, provides protection from disease progression. an indirect haemagglutination assay was used to detect antibodies to b. pseudomallei in 1500 healthy donors in an endemic region of australia. lymphocyte proliferation, activation and cytokine expression to b. pseudomallei antigen were determined in eight donors who were seropositive and in eight age- and sex-matched controls. in north queensland, 2.5% of the pop ... | 2008 | 19121692 |
| burkholderia pseudomallei enhances maturation of bone marrow-derived dendritic cells. | t-cell activation is essential for protection against burkholderia pseudomallei infection. using bone marrow-derived dendritic cells (bmdc) isolated from partially resistant c57bl/6 and susceptible balb/c mice, the degree of bmdc activation in the presence of b. pseudomallei was investigated. maturation, cytokine production and internalization of b. pseudomallei by bmdc was assessed in response to infection with a highly virulent and a low-virulent clinical isolate. maturation was determined by ... | 2008 | 19121693 |
| activation of human monocyte-derived dendritic cells by burkholderia pseudomallei does not require binding to the c-type lectin dc-sign. | dendritic cells (dcs) are essential in regulating adaptive immunity. dc-sign (dc-specific icam-grabbing nonintegrin) is a c-type lectin receptor that is expressed mainly by dcs. accumulating evidence supports that certain pathogens target dc-sign to escape host immunity. to investigate a possible role of dc-sign in burkholderia pseudomallei infection, we initially screened its dc-sign binding activity by an elisa method utilizing a dc-sign-fc chimeric protein and found that all of the b. pseudom ... | 2008 | 19121694 |
| the effect of different burkholderia pseudomallei isolates of varying levels of virulence on toll-like-receptor expression. | the purpose of this investigation was to ascertain the degree of toll-like-receptor (tlr) activation by burkholderia pseudomallei isolates with varying levels of virulence 2 h post infection. standard antibiotic protection assays were performed on raw 264.7 macrophages and peritoneal exudate cells (pec) challenged with b. pseudomallei. real-time pcr (rt-pcr) was performed to determine tlr2, tlr4, tlr5 and tlr9 expression. internalization and killing of bacteria were determined 2h post infection. ... | 2008 | 19121695 |
| induction of protective immunity against burkholderia pseudomallei using attenuated mutants with defects in the intracellular life cycle. | melioidosis is a severe infectious disease caused by the gram-negative rod burkholderia pseudomallei. there is currently no vaccine available. we recently generated and characterized several highly attenuated transposon mutants with defects in the intracellular life cycle of b. pseudomallei. in the present study we examined the protective effects of six of these mutants: four harbouring knockouts in genes involved in several biosynthetic pathways (purn(-), purm(-), hisf(-), pabb(-)); a putative ... | 2008 | 19121696 |
| tbk1 does not play a role in the control of in vitro burkholderia pseudomallei growth. | burkholderia pseudomallei, the causative agent of melioidosis, is an important intracellular pathogen in tropical regions. tank-binding kinase (tbk1), part of the pathway that induces transcription of type i interferon genes, has been demonstrated to play an important role in controlling intracellular bacterial infections. to investigate the role of tbk1 in protecting against b. pseudomallei we developed tbk1-deficient cell lines by using shrna for transient knockdown of the tbk1 gene in hela an ... | 2008 | 19121697 |
| generation of murine bone marrow derived macrophages in a standardised serum-free cell culture system. | murine bone marrow derived macrophages (bmm) are valuable tools to investigate macrophage functions such as cytokine production and bactericidal activities from different strains of mice. in most studies bmm are generated and characterised using cell culture systems with fetal calf serum (fcs) as an essential supplement. since serum contains varying amounts of undefined components influencing the maturation and polarisation process of bmm there is a need for a more standardised methodology. the ... | 2009 | 19133267 |
| melioidosis risk in a tropical industrial environment. | an investigation into the risk of occupationally acquired melioidosis at a mine site in northern australia found that 45 (13%) of 345 staff had serologic evidence of exposure and 14 (4%) had recent exposure to burkholderia pseudomallei or closely related bacteria. there was only one culture-confirmed case of melioidosis in mine staff during the study period. the lack of overt infection directly attributable to work activities despite detectable b. pseudomallei on site, the absence of an associat ... | 2009 | 19141844 |
| facile construction of unmarked deletion mutants in burkholderia pseudomallei using sacb counter-selection in sucrose-resistant and sucrose-sensitive isolates. | burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. here we report unmarked allele-replacement mutagenesis using efficient sacb counter-selection. despite being genotypically sacb(+), most commonly used b. pseudomallei strains are sucrose-resistant and efficient sacb counter-selection is demonstrated in both resistant and sensitive strains. | 2009 | 19150470 |
| on the trail of disease in southeast asia. | 2009 | 19153251 | |
| melioidosis and tuberculosis: dual pathogens in a neck abscess. | melioidosis is an infectious disease caused by a saprophytic bacterium, burkholderia pseudomallei. it is endemic to southeast asia and northern australia. it may manifest as a pulmonary lesion, osteomyelitis, abscesses in soft tissue and various organs, or as septicaemia. | 2009 | 19154649 |
| landscape changes influence the occurrence of the melioidosis bacterium burkholderia pseudomallei in soil in northern australia. | the soil-dwelling saprophyte bacterium burkholderia pseudomallei is the cause of melioidosis, a severe disease of humans and animals in southeast asia and northern australia. despite the detection of b. pseudomallei in various soil and water samples from endemic areas, the environmental habitat of b. pseudomallei remains unclear. | 2009 | 19156200 |
| in vitro interactions between cotrimoxazole and doxycycline in burkholderia pseudomallei: how important is this combination in maintenance therapy of melioidosis? | 2009 | 19172079 | |
| protection from pneumonic infection with burkholderia species by inhalational immunotherapy. | burkholderia mallei and b. pseudomallei are important human pathogens and cause the diseases glanders and melioidosis, respectively. both organisms are highly infectious when inhaled and are inherently resistant to many antimicrobials, thus making it difficult to treat pneumonic burkholderia infections. we investigated whether it was possible to achieve rapid protection against inhaled burkholderia infection by using inhaled immunotherapy. for this purpose, cationic liposome dna complexes (cldc) ... | 2009 | 19179415 |
| caspase-1 mediates resistance in murine melioidosis. | the gram-negative rod burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease which is endemic in tropical and subtropical areas. the bacterium multiplies intracellularly within the cytosol, induces the formation of actin tails, and can spread directly from cell to cell. recently, it has been shown that b. pseudomallei can induce caspase-1-dependent cell death in macrophages. the aim of the present study was to further elucidate the role of caspase-1 during b ... | 2009 | 19179418 |
| melioidosis after brief exposure: a serologic survey in us marines. | melioidosis is endemic to southeast asia. the incidence of infection in visitors is not well known, especially for short visits. thirteen (38%) of 34 previously unexposed us marines had positive serology after 2 weeks in thailand, and one developed acute disseminated disease. asymptomatic infection with burkholderia pseudomallei may be common, even from brief exposures. | 2009 | 19190209 |
| melioidosis masquerading as community-acquired pneumonia: a case report demonstrating efficacy of intrapleural fibrinolytic therapy. | burkholderia pseudomallei, a soil saprophyte typically associated with the highly fatal disease melioidosis in southeast asia, is an increasing global concern as worldwide travel proliferates in our increasingly cosmopolitan society. emergence of this disease in locations where it was not previously described underscores the importance of understanding the basic microbiology, pathogenesis, and epidemiology of infectious diseases on a global scale rather than the myopic view of the usual microbio ... | 2009 | 19192137 |
| inactivation of virulent burkholderia pseudomallei by sunlight. | the goal of this study was to determine the sensitivity of virulent burkholderia pseudomallei to natural sunlight. we describe solar dosimetry calibrated to integrate radiation between 295 and 305 nm and an exposure system that minimizes thermal effects on bacterial cells. burkholderia pseudomallei cells were either exposed to sunlight in uv transparent dishes or maintained in the dark covered by opaque foil. the cells maintained in the dark remained at constant levels for the duration of all ex ... | 2009 | 19192198 |
| identification of melioidosis outbreak by multilocus variable number tandem repeat analysis. | endemic melioidosis is caused by genetically diverse burkholderia pseudomallei strains. however, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. b. pseudomallei is designated a group b bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) can identify genetically related isolates, but results take several days to obtain. we develo ... | 2009 | 19193259 |
| genetics and function of the capsules of burkholderia pseudomallei and their potential as therapeutic targets. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal disease that is endemic to southeast asia and northern australia. the clinical manifestations of melioidosis may range from an acute pneumonia or acute septicemia, to chronic and latent infections. b. pseudomallei is inherently resistant to a number of antibiotics, and even with aggressive antibiotic therapy, the mortality rate remains high, and the incidence of relapse is common. the resistance of this organism to a number ... | 2009 | 19200030 |
| experimental acute respiratory burkholderia pseudomallei infection in balb/c mice. | burkholderia pseudomallei is the causative agent of melioidosis, which is considered a potential deliberate release agent. the objective of this study was to establish and characterise a relevant, acute respiratory burkholderia pseudomallei infection in balb/c mice. mice were infected with 100 b. pseudomallei strain bri bacteria by the aerosol route (approximately 20 median lethal doses). bacterial counts within lung, liver, spleen, brain, kidney and blood over 5 days were determined and histopa ... | 2009 | 19200247 |
| kirbac1.1: it's an inward rectifying potassium channel. | kirbac1.1 is a prokaryotic homologue of eukaryotic inward rectifier potassium (kir) channels. the crystal structure of kirbac1.1 and related kirbac3.1 have now been used extensively to generate in silico models of eukaryotic kir channels, but functional analysis has been limited to (86)rb(+) flux experiments and bacteria or yeast complementation screens, and no voltage clamp analysis has been available. we have expressed pure full-length his-tagged kirbac1.1 protein in escherichia coli and obtai ... | 2009 | 19204189 |
| a bacterial type iii effector family uses the papain-like hydrolytic activity to arrest the host cell cycle. | pathogenic bacteria deliver effector proteins into host cells through the type iii secretion apparatus to modulate the host function. we identify a family of proteins, homologous to the type iii effector cif from enteropathogenic escherichia coli, in pathogens including yersinia, photorhabdus, and burkholderia that contain functional type iii secretion systems. like cif, this family of proteins is capable of arresting the host cell cycle at g(2)/m. structure of one of the family members, cif hom ... | 2009 | 19225106 |
| a role for carbapenem in the treatment of melioidosis in developing countries? | 2009 | 19231268 | |
| burkholderia pseudomallei recovered in an hiv-positive individual. | 2008 | 19265236 | |
| [fluoroquinolones: antimicrobial activity and chemotherapeutic efficacy with respect to various pathogens in highly dangerous diseases]. | comparative in vitro and in vivo efficacy of new fluoroquinolones with respect to pathogens of tularemia, glanders, melioidosis and anthrax was estimated. it was shown that the strains of the tularemia, glanders, melioidosis and anthrax pathogens were in vitro highly susceptible to the new agents. the experiments on laboratory animals demonstrated that pefloxacin and sparfloxacin had extremely broad spectra and were of special value in emergency prophylaxis of tularemia, glanders, melioidosis an ... | 2007 | 19275053 |
| immunity to burkholderia pseudomallei. | largely due to its recognition as a biological threat agent, current knowledge on melioidosis, caused by the gram-negative bacterium burkholderia pseudomallei, has increased tremendously over the last years. this review summarizes current understanding on the molecular characterization of b. pseudomallei and the immunology of melioidosis. | 2009 | 19276877 |
| simple sequence repeat (ssr)-based gene diversity in burkholderia pseudomallei and burkholderia mallei. | pathogens burkholderia pseudomallei (bp) and burkholderia mallei (bm) contain a large number (> 12,000) of simple sequence repeats (ssrs). to study the extent to which these features have contributed to the diversification of genes, we have conducted comparative studies with nineteen genomes of these bacteria. we found 210 genes with characteristic types of ssr variations. ssrs with nonamer repeat units were the most abundant, followed by hexamers and trimers. amino acids with smaller and nonpol ... | 2009 | 19277507 |
| comparison of routine bench and molecular diagnostic methods in identification of burkholderia pseudomallei. | this study compared the identification of burkholderia pseudomallei with that of related organisms. bench tests and latex agglutination were compared with molecular identification. using bench tests and latex agglutination alone, 100% (30/30) of b. pseudomallei isolates were correctly identified. amoxicillin-clavulanate susceptibility testing was also a good and simple discriminatory test. | 2009 | 19279182 |
| mechanistic insight into the initiation step of the reaction of burkholderia pseudomallei catalase-peroxidase with peroxyacetic acid. | the reaction of the catalase-peroxidase of burkholderia pseudomallei with peroxyacetic acid has been analyzed using stopped-flow spectrophotometry. two well-defined species were observed, the first defined by an increase in intensity and narrowing of the soret band at 407 nm and a 10-nm shift of the charge transfer band from 635 to 625 nm. these features are consistent with a ferric spectrum with a greater proportion of sixth-coordination character and are assigned to an fe(iii)-peroxyacetic aci ... | 2009 | 19290552 |
| variations in ceftazidime and amoxicillin-clavulanate susceptibilities within a clonal infection of burkholderia pseudomallei. | a patient with a clonal infection of burkholderia pseudomallei had subpopulations with ceftazidime and amoxicillin-clavulanate susceptibilities that differed among the clinical specimens. resistance was associated with a novel cys69tyr substitution in the ambler class a beta-lactamase. susceptibility testing of multiple colony variants from different sites should be performed for patients with culture-confirmed melioidosis. | 2009 | 19297597 |
| phenotypic characteristics and pathogenic ability across distinct morphotypes of burkholderia pseudomallei dt. | burkholderia pseudomallei dt is unusual as it exhibits six distinct colony morphotypes. types iii and v show stronger motility, whereas type vi exhibits the highest levels of bacterial association with peritoneal exudate cells. although the bacterial loads in the organs are not significantly different for infections by the six distinct morphotypes, higher mortality (100% and 89%, respectively) and larger areas of abnormal liver debris (20.6% and 22.4%, respectively) are found with types i- and i ... | 2009 | 19302529 |
| cycle inhibiting factors (cifs) are a growing family of functional cyclomodulins present in invertebrate and mammal bacterial pathogens. | the cycle inhibiting factor (cif) produced by enteropathogenic and enterohemorrhagic escherichia coli was the first cyclomodulin to be identified that is injected into host cells via the type iii secretion machinery. cif provokes cytopathic effects characterized by g(1) and g(2) cell cycle arrests, accumulation of the cyclin-dependent kinase inhibitors (ckis) p21(waf1/cip1) and p27(kip1) and formation of actin stress fibres. the x-ray crystal structure of cif revealed it to be a divergent member ... | 2009 | 19308257 |
| expression and purification of 30 kilodalton protein antigen of ara- burkholderia pseudomallei. | the 30 kda protein of b. pseudomallei is found in virulent ara- but not avirulent ara+ strain. the gene was cloned in escherichia coli jm105 employing piniii-c2 vector. the open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. arginine was the most abundant amino acid in the protein, having a pi of 12.65. nucleotide sequence of the gene was 96% identical to b. pseudomallei 1710b chromosome ii sequence cp000125.1, encoding an oxidoreductase of the short chain dehyd ... | 2009 | 19323014 |
| construction and characterization of an unmarked aroc deletion mutant of burkholderia pseudomallei strain a2. | using a sucrose counter-selection strategy, we have constructed an unmarked aroc deletion mutant of b. pseudomallei strain a2 in order to investigate the possibility of an effective live-attenuated vaccine. the aroc deletion in the resultant mutant, designated a2deltaaroc, was confirmed by pcr, southern hybridization and failure of the mutant to grow in a defined medium without aromatic compounds. compared to the parental wild type strain, a2deltaaroc was highly attenuated for virulence followin ... | 2009 | 19323044 |
| validation of ten new polymorphic tandem repeat loci and application to the mlva typing of burkholderia pseudomallei isolates collected in singapore from 1988 to 2004. | multiple locus variable number of tandem repeat (vntr) analysis (mlva) has been shown to be very promising for the typing of burkholderia pseudomallei and mallei. the currently available set of loci requires high resolution allele size measurement due to short repeat units. the present work was aimed at expanding the available set of vntr loci, and generating data from a collection of 102 b. pseudomallei strains isolated in singapore between 1988 and 2004 including few additional strains of vari ... | 2009 | 19327380 |
| altered secretome of burkholderia pseudomallei induced by salt stress. | burkholderia pseudomallei is a saprophyte found in soil and water. it is a difficult microorganism to kill and can survive in these environments for many years. mechanisms for its adaptive response to environmental changes remain largely unknown. we performed a proteomics study to examine alterations in secreted proteins (secretome) under a salt stress (with 150 mm nacl) compared to the normal cultured condition in lb broth. the culture supernatants were filtrated and precipitated with 50% ethan ... | 2009 | 19336033 |
| phenotypic and functional characterization of human memory t cell responses to burkholderia pseudomallei. | infection with the gram-negative bacterium burkholderia pseudomallei is an important cause of community-acquired lethal sepsis in endemic regions in southeast asia and northern australia and is increasingly reported in other tropical areas. in animal models, production of interferon-gamma (ifn-gamma) is critical for resistance, but in humans the characteristics of ifn-gamma production and the bacterial antigens that are recognized by the cell-mediated immune response have not been defined. | 2009 | 19352426 |
| identification and characterization of rpos regulon and rpos-dependent promoters in burkholderia pseudomallei. | rpos subunit of rna polymerase is a bacterial alternative sigma factor and major regulator important for response to a variety of stress conditions. however, rpos-dependent genes in burkholderia pseudomallei remained undefined. we identified the rpos regulon of b. pseudomallei using a proteomics approach, which revealed 70 differentially expressed proteins between rpos(+) and rpos(-) strains. the rpos-dependent genes were then classified into 14 functional categories, most of which were related ... | 2009 | 19364128 |
| in vitro activity of doripenem against burkholderia pseudomallei. | the mic(50) and mic(90) values of doripenem, determined by etest, for 110 isolates of burkholderia pseudomallei were 0.5 and 0.75 microg/ml, respectively. there were significant correlations between mics determined by etest and mics determined by agar dilution, mics determined by etest and inhibition zone size, and mics determined by agar dilution and inhibition zone size. | 2009 | 19364859 |
| dna microarray-based detection and identification of burkholderia mallei, burkholderia pseudomallei and burkholderia spp. | we developed a rapid oligonucleotide microarray assay based on genetic markers for the accurate identification and differentiation of burkholderia (b.) mallei and burkholderia pseudomallei, the agents of glanders and melioidosis, respectively. these two agents were clearly identified using at least 4 independent genetic markers including 16s rrna gene, flic, motb and also by novel species-specific target genes, identified by in silico sequence analysis. specific hybridization signal profiles all ... | 2009 | 19366627 |
| life on the inside: the intracellular lifestyle of cytosolic bacteria. | bacterial pathogens exploit a huge range of niches within their hosts. many pathogens can invade non-phagocytic cells and survive within a membrane-bound compartment. however, only a small number of bacteria, including listeria monocytogenes, shigella flexneri, burkholderia pseudomallei, francisella tularensis and rickettsia spp., can gain access to and proliferate within the host cell cytosol. here, we discuss the mechanisms by which these cytosolic pathogens escape into the cytosol, obtain nut ... | 2009 | 19369949 |
| quorum-sensing control of antibiotic synthesis in burkholderia thailandensis. | the genome of burkholderia thailandensis codes for several luxr-luxi quorum-sensing systems. we used b. thailandensis quorum-sensing deletion mutants and recombinant escherichia coli to determine the nature of the signals produced by one of the systems, btar2-btai2, and to show that this system controls genes required for the synthesis of an antibiotic. btai2 is an acyl-homoserine lactone (acyl-hsl) synthase that produces two hydroxylated acyl-hsls, n-3-hydroxy-decanoyl-hsl (3ohc(10)-hsl) and n- ... | 2009 | 19376863 |
| engineering of tellurite-resistant genetic tools for single-copy chromosomal analysis of burkholderia spp. and characterization of the burkholderia thailandensis betba operon. | there are few appropriate single-copy genetic tools for most burkholderia species, and the high level of antibiotic resistance in this genus further complicates the development of genetic tools. in addition, the utilization of resistance genes for clinically important antibiotics is prohibited for the bioterrorism agents burkholderia pseudomallei and burkholderia mallei, necessitating the development of additional nonantibiotic-based genetic tools. three single-copy systems devoid of antibiotic ... | 2009 | 19376905 |
| differential intracellular fate of burkholderia pseudomallei 844 and burkholderia thailandensis ue5 in human monocyte-derived dendritic cells and macrophages. | burkholderia pseudomallei (bp) is a category b biothreat organism that causes a potentially fatal disease in humans and animals, namely melioidosis. burkholderia thailandensis (bt) is another naturally occurring species that is very closely related to bp. however, despite this closely related genotype, bt is considered avirulent as it does not cause the disease. in the present study, we compared the growth kinetics of b. pseudomallei strain 844 (bp-844) in human monocyte-derived dendritic cells ... | 2009 | 19397822 |
| clinical features that affect indirect-hemagglutination-assay responses to burkholderia pseudomallei. | melioidosis, a disease endemic to northern australia and southeast asia, is caused by the soil saprophyte burkholderia pseudomallei. the indirect hemagglutination assay (iha) is the most frequently used serological test to help confirm exposure to the causative organism. however, despite culture-confirmed disease, patients often have a negative iha result at presentation and occasionally fail to seroconvert in serial testing. we retrospectively examined results for all patients with culture-conf ... | 2009 | 19403784 |
| [a case of disseminated melioidosis in a migrant worker from thailand]. | with globalization, foreign patients are frequently encountered at the clinical practice in korea. as the number of migrant workers from southeast asia has been notably rising since the late 1990's, unfamiliar tropical infectious diseases that they bring out, may give great challenges to the routine clinical practice in korea. melioidosis is a community-acquired infection caused by a gram-negative bacillus, burkholderia pseudomallei. it has been endemic in southeast asia and northern australia, ... | 2009 | 19411781 |
| melioidotic prostatic abscess in pahang. | melioidosis is caused by burkholderia pseudomallei, a gram-negative bacillus. melioidosis can affect many organs, including the prostate. however, prostatic abscess due to melioidosis is uncommon. we describe five cases of melioidosis with prostatic abscess. four of five patients had diabetes mellitus and had more than one organ involvement. the diagnosis of prostatic abscess in our patients was only made with computed tomography of the abdomen and pelvis. none of our patients underwent surgical ... | 2009 | 19421682 |
| primary ocular melioidosis due to a single genotype of burkholderia pseudomallei in two cats from arnhem land in the northern territory of australia. | melioidosis was diagnosed in two domestic crossbred cats presented for unilateral ocular disease. one patient was born and bred in nhulunbuy, arnhem land, while the other had moved there 6 months previously from townsville, queensland. both patients were presented with sudden onset of a 'red eye' and blepharospasm, which progressed to an enlarged, painful, firm globe with loss of pupillary light reflexes and vision. an obvious primary focus of infection outside the eye was not detected in either ... | 2009 | 19428280 |
| the rho gtpase inactivation domain in vibrio cholerae martx toxin has a circularly permuted papain-like thiol protease fold. | a rho gtpase inactivation domain (rid) has been discovered in the multifunctional, autoprocessing rtx toxin rtxa from vibrio cholerae. the rid domain causes actin depolymerization and rounding of host cells through inactivation of the small rho gtpases rho, rac, and cdc42. with only a few toxin proteins containing rid domains in the current sequence database, the structure and molecular mechanisms of this domain are unknown. using comparative sequence and structural analyses, we report homology ... | 2009 | 19434753 |
| crystal structures of cif from bacterial pathogens photorhabdus luminescens and burkholderia pseudomallei. | a pre-requisite for bacterial pathogenesis is the successful interaction of a pathogen with a host. one mechanism used by a broad range of gram negative bacterial pathogens is to deliver effector proteins directly into host cells through a dedicated type iii secretion system where they modulate host cell function. the cycle inhibiting factor (cif) family of effector proteins, identified in a growing number of pathogens that harbour functional type iii secretion systems and have a wide host range ... | 2009 | 19440549 |
| global optimal eburst analysis of multilocus typing data using a graphic matroid approach. | multilocus sequence typing (mlst) is a frequently used typing method for the analysis of the clonal relationships among strains of several clinically relevant microbial species. mlst is based on the sequence of housekeeping genes that result in each strain having a distinct numerical allelic profile, which is abbreviated to a unique identifier: the sequence type (st). the relatedness between two strains can then be inferred by the differences between allelic profiles. for a more comprehensive an ... | 2009 | 19450271 |
| burkholderia pseudomallei tropism and the melioidosis road map. | 2009 | 19456227 | |
| nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for burkholderia pseudomallei in murine melioidosis. | burkholderia pseudomallei, the causative agent of melioidosis, is generally considered to be acquired via inhalation of dust or water droplets from the environment. in this study, we show that infection of the nasal mucosa is potentially an important portal of entry in melioidosis. | 2009 | 19456230 |
| [development of a multiplex pcr-suspension array for simultaneous detection of five bioterrorism bacteria]. | to develop a rapid, high-throughput screening method of gene suspension array technique to simultaneously detect five bioterrorism bacteria: bacillus anthracis, francisella tularensis, yersinia pestis, brucella spp. and burkholderia pseudomallei. | 2009 | 19462919 |
| comparison of four selective media for the isolation of burkholderia mallei and burkholderia pseudomallei. | currently there are no commercially available selective media indicated for the isolation of burkholderia mallei and burkholderia pseudomallei. ashdown's agar, a custom selective medium for isolation of b. pseudomallei, is well described in the literature but unavailable commercially. three commercially available media, burkholderia cepacia selective agar (bcsa), oxidative-fermentative-polymyxin b-bacitracin-lactose (ofpbl) agar, and pseudomonas cepacia (pc) agar are recommended for isolation of ... | 2009 | 19478269 |
| positive serologic test results for burkholderia pseudomallei in asymptomatic persons. | 2009 | 19478278 | |
| protective efficacy of heat-inactivated b. thailandensis, b. mallei or b. pseudomallei against experimental melioidosis and glanders. | burkholderia pseudomallei and burkholderia mallei are gram-negative bacilli that are the causative agents of melioidosis and glanders, respectively. both humans and animals are susceptible to both diseases. there is currently no vaccine available for the prevention of disease. we report the protective efficacy of heat-inactivated burkholderia thailandensis, b. mallei or b. pseudomallei cells as vaccines against murine melioidosis and glanders. immunisation with heat-inactivated b. pseudomallei c ... | 2009 | 19490962 |
| comparative analysis of extracellular enzymes and virulence exhibited by burkholderia pseudomallei from different sources. | to evaluate the potential role of extracellular proteins in the pathogenicity and virulence of burkholderia pseudomallei, the activities of several enzymes in the culture filtrates of nine clinical and six environmental isolates were investigated in vitro and in vivo in icr strain of mice. the production of protease, phosphatase, phospholipase c, superoxide dismutase, catalase and peroxidase were detected in the culture filtrates of all the 15 isolates at different time points of growth 4-24h. o ... | 2009 | 19524661 |
| prostatic abscess due to burkholderia pseudomallei: 81 cases from a 19-year prospective melioidosis study. | we review the epidemiology, clinical presentation, management and outcome of prostatic abscess secondary to burkholderia pseudomallei infection in the northern territory, australia. we highlight the frequency of prostatic abscess occurrence in melioidosis, and formulate recommendations for its detection and management. | 2009 | 19524969 |
| distinct role of specific tryptophans in facilitating electron transfer or as [fe(iv)=o trp(*)] intermediates in the peroxidase reaction of bulkholderia pseudomallei catalase-peroxidase: a multifrequency epr spectroscopy investigation. | we have characterized the reactive intermediates of the peroxidase-like reaction of bulkholderia pseudomallei katg using multifrequency epr spectroscopy. the aim was to investigate the putative role of tryptophanyl radicals as alternative intermediates to the [fe(iv)=o por(*+)] species or as short-lived species involved in superexchange-coupled pathways between redox cofactors. three distinct sites for the formation of radical intermediates, trp330, trp139 and trp153, were identified using singl ... | 2009 | 19530730 |
| prevalence of burkholderia pseudomallei in guangxi, china. | melioidosis, an infectious disease caused by the gram-negative bacterium burkholderia pseudomallei, is now recognized as an important public health problem in southeast asia and tropical northern australia. although b. pseudomallei has been detected in various water and soil samples in southeast china, the enviromental distribution of b. pseudomallei in china is unclear. in the winter months of 2007, 154 and 130 soil and water samples, respectively, were collected from several locations in guang ... | 2010 | 19538822 |
| variability of burkholderia pseudomallei strain sensitivities to chlorine disinfection. | burkholderia pseudomallei is a select agent and the causative agent of melioidosis. variations in previously reported chlorine and monochloramine concentration time (ct) values for disinfection of this organism make decisions regarding the appropriate levels of chlorine in water treatment systems difficult. this study identified the variation in ct values for 2-, 3-, and 4-log(10) reductions of eight environmental and clinical isolates of b. pseudomallei in phosphate-buffered water. the greatest ... | 2009 | 19542324 |
| differential gene expression profiles of lung epithelial cells exposed to burkholderia pseudomallei and burkholderia thailandensis during the initial phase of infection. | burkholderia pseudomallei is the causative agent of melioidosis, and its infection usually affects patients' lungs. the organism is a facultative intracellular gram-negative bacillus commonly found in soil and water in endemic tropical regions. another closely related burkholderia species found in soil and water is b. thailandensis. this bacterium is a non-pathogenic environmental saprophyte. b. pseudomallei is considerably more efficient than b. thailandensis in host cell invasion and adherence ... | 2009 | 19548631 |
| comparative in vivo and in vitro analyses of putative virulence factors of burkholderia pseudomallei using lipopolysaccharide, capsule and flagellin mutants. | burkholderia pseudomallei is a gram-negative bacillus that is the causative agent of melioidosis. we evaluated host-pathogen interaction at different levels using three separate b. pseudomallei mutants generated by insertional inactivation. one of these mutants is defective in the production of the polysaccharide side chains associated with lipopolysaccharide; one does not produce the capsular polysaccharide with the structure -3)-2-o-acetyl-6-deoxy-beta-d-manno-heptopyranose-(1-; and the third ... | 2009 | 19549172 |
| a genetic programming approach for burkholderia pseudomallei diagnostic pattern discovery. | motivation: finding diagnostic patterns for fighting diseases like burkholderia pseudomallei using biomarkers involves two key issues. first, exhausting all subsets of testable biomarkers (antigens in this context) to find a best one is computationally infeasible. therefore, a proper optimization approach like evolutionary computation should be investigated. second, a properly selected function of the antigens as the diagnostic pattern which is commonly unknown is a key to the diagnostic accurac ... | 2009 | 19561021 |
| in vitro susceptibility of burkholderia pseudomallei to antimicrobial peptides. | burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many antibiotics, resulting in high mortality rates of 19% in australia and even 50% in thailand. antimicrobial peptides (amps) possess potent broad-spectrum bactericidal activities and are regarded as promising therapeutic alternatives in the fight against resistant microorganisms. moreover, these peptides may also affect inflammation, immune activation and wound healing. in this study, the in vitro act ... | 2009 | 19577435 |
| the public health implications of melioidosis. | melioidosis, which is caused by the bacterium burkholderia pseudomallei, is a potentially fatal tropical infection, little known outside its main endemic zone of southeast asia and northern australia. though it has received more attention in recent years on account of its claimed suitability as a biological weapon agent, the principal threat from melioidosis is a result of naturally occurring events. occasional case clusters, sporadic cases outside the known endemic zone and infections in unusua ... | 2009 | 19578632 |
| neonatal melioidosis: a case report from india. | melioidosis, caused by burkholderia pseudomallei , is an infectious disease of major public health importance in southeast asia and australia. we report, for the first time from the indian subcontinent, a case of melioidosis in a neonate, its clinical presentation, microbiological diagnosis, possible mode of transmission and outcome. a pre-term female baby developed respiratory distress soon after birth. the child was febrile, had tachypnea, grunting, normal heart rate with a low pulse volume an ... | 2009 | 19584512 |
| pathogenesis of burkholderia pseudomallei and burkholderia mallei. | burkholderia pseudomallei and mallei are biological agents of military significance. there has been significant research in recent years to develop medical countermeasures for these organisms. this review summarizes work which details aspects of the pathogenesis of b. pseudomallei and mallei and discusses key scientific questions and directions for future research. | 2009 | 19585782 |
| dosing regimens of cotrimoxazole (trimethoprim-sulfamethoxazole) for melioidosis. | melioidosis is an infectious disease with a propensity for relapse, despite prolonged antibiotic eradication therapy for 12 to 20 weeks. a pharmacokinetic (pk) simulation study was performed to determine the optimal dosing of cotrimoxazole (trimethoprim-sulfamethoxazole [tmp-smx]) used in current eradication regimens in thailand and australia. data for bioavailability, protein binding, and coefficients of absorption and elimination were taken from published literature. apparent volumes of distri ... | 2009 | 19620336 |
| burkholderia pseudomallei isocitrate lyase is a persistence factor in pulmonary melioidosis: implications for the development of isocitrate lyase inhibitors as novel antimicrobials. | burkholderia pseudomallei, the causative agent of melioidosis, has often been called the great "mimicker," and clinical disease due to this organism may include acute, chronic, and latent pulmonary infections. interestingly, chronic pulmonary melioidosis is often mistaken for tuberculosis, and this can have significant consequences, as the treatments for these two infections are radically different. the recurrent misdiagnosis of melioidosis for tuberculosis has caused many to speculate that thes ... | 2009 | 19620343 |
| mutational analysis of burkholderia thailandensis quorum sensing and self-aggregation. | acyl-homoserine lactone (acyl-hsl) quorum-sensing signaling is common to many proteobacteria. acyl-hsls are synthesized by the luxi family of synthases, and the signal response is mediated by members of the luxr family of transcriptional regulators. burkholderia thailandensis is a member of a closely related cluster of three species, including the animal pathogens burkholderia mallei and burkholderia pseudomallei. members of this group have similar luxi and luxr homologs, and these genes contrib ... | 2009 | 19648250 |
| glyphosate resistance as a novel select-agent-compliant, non-antibiotic-selectable marker in chromosomal mutagenesis of the essential genes asd and dapb of burkholderia pseudomallei. | genetic manipulation of the category b select agents burkholderia pseudomallei and burkholderia mallei has been stifled due to the lack of compliant selectable markers. hence, there is a need for additional select-agent-compliant selectable markers. we engineered a selectable marker based on the gat gene (encoding glyphosate acetyltransferase), which confers resistance to the common herbicide glyphosate (gs). to show the ability of gs to inhibit bacterial growth, we determined the effective conc ... | 2009 | 19648360 |
| burkholderia pseudomallei is genetically diverse in agricultural land in northeast thailand. | the soil-dwelling gram-negative bacterium burkholderia pseudomallei is the cause of melioidosis. extreme structuring of genotype and genotypic frequency has been demonstrated for b. pseudomallei in uncultivated land, but its distribution and genetic diversity in agricultural land where most human infections are probably acquired is not well defined. | 2009 | 19652701 |
| immunogenic burkholderia pseudomallei outer membrane proteins as potential candidate vaccine targets. | burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. there is no vaccine towards the bacterium available in the market, and the efficacy of many of the bacterium's surface and secreted proteins are currently being evaluated as vaccine candidates. | 2009 | 19654871 |
| a burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens. | understanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. to provide insight into this area, we fabricated a protein microarray containing 1,205 burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, and identified 170 reactive antigens. this subset of antigens was printed on a smaller array and probed with a collection of 747 individual sera derived from 10 patient groups including melioidosis ... | 2009 | 19666533 |
| a diagnosis of burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. | melioidosis is an infectious disease caused by the gram-negative bacterium burkholderia pseudomallei. interest in the molecular identification of b. pseudomallei has increased after its classification as a category b agent by the us centers for disease control and prevention. the present article reports a diagnosis of b. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. the results obtained show that direct detection of the 16-23s spacer sequence in br ... | 2009 | 19679240 |
| structural and biological diversity of lipopolysaccharides from burkholderia pseudomallei and burkholderia thailandensis. | burkholderia pseudomallei, the etiological agent of melioidosis, is a facultative intracellular pathogen. as b. pseudomallei is a gram-negative bacterium, its outer membrane contains lipopolysaccharide (lps) molecules, which have been shown to have low-level immunological activities in vitro. in this study, the biological activities of b. pseudomallei lps were compared to those of burkholderia thailandensis lps, and it was found that both murine and human macrophages produced levels of tumor nec ... | 2009 | 19692625 |
| versatile dual-technology system for markerless allele replacement in burkholderia pseudomallei. | burkholderia pseudomallei is the etiologic agent of melioidosis, a rare but serious tropical disease. in the united states, genetic research with this select agent bacterium is strictly regulated. although several select agent compliant methods have been developed for allelic replacement, all of them suffer from some drawbacks, such as a need for specific host backgrounds or use of minimal media. here we describe a versatile select agent compliant allele replacement system for b. pseudomallei ba ... | 2009 | 19700544 |
| identification of burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody. | the genus burkholderia includes many bacteria that cause serious human infections. as is the case with other gram-negative bacteria, burkholderia species produce lps, which is an abundant component of the bacterial cell surface. burkholderia cepacia complex (bcc) bacteria (which include at least 17 separate species) produce lps structures that are quite different. in an attempt to determine the degree of lps epitope variation among bcc species, a mab was produced, designated 5d8, specific for th ... | 2010 | 19729457 |
| the molecular and cellular basis of pathogenesis in melioidosis: how does burkholderia pseudomallei cause disease? | melioidosis, a febrile illness with disease states ranging from acute pneumonia or septicaemia to chronic abscesses, was first documented by whitmore & krishnaswami (1912). the causative agent, burkholderia pseudomallei, was subsequently identified as a motile, gram-negative bacillus, which is principally an environmental saprophyte. melioidosis has become an increasingly important disease in endemic areas such as northern thailand and australia (currie et al., 2000). this health burden, plus th ... | 2009 | 19732156 |
| melioidosis in south-eastern queensland. | 2009 | 19740059 | |
| role for the burkholderia pseudomallei capsular polysaccharide encoded by the wcb operon in acute disseminated melioidosis. | the capsular polysaccharide of burkholderia pseudomallei is an essential virulence determinant that is required for protection from host serum cidal activity and opsonophagocytosis. in this study, the immune response directed against a b. pseudomallei capsule mutant (jw270) was investigated in an acute respiratory murine model. jw270 was significantly attenuated in this model ( approximately 2 logs) to levels resembling those of avirulent burkholderia thailandensis. at lethal doses, jw270 coloni ... | 2009 | 19752033 |
| molecular basis of rare aminoglycoside susceptibility and pathogenesis of burkholderia pseudomallei clinical isolates from thailand. | burkholderia pseudomallei is intrinsically resistant to aminoglycosides and macrolides, mostly due to amrab-opra efflux pump expression. we investigated the molecular mechanisms of aminoglycoside susceptibility exhibited by thai strains 708a, 2188a, and 3799a. | 2009 | 19771149 |
| proceedings from the 5th world melioidosis congress. khon kaen, thailand. november 21-23, 2007. | 2008 | 19787859 | |
| diagnosis of endotoxemia with gram-negative bacteremia is bacterial species dependent: a meta-analysis of clinical studies. | endotoxemia is undetectable for up to 60% of cases of bacteremia caused by gram-negative (gn) species, a discordance attributed to the limitations of the limulus assay for endotoxemia. the lipid a structure of the endotoxin molecule is critical for the sensing of gn bacteria by the host immune system although not so for sensing by the limulus assay. the lipid a structure of commensal enterobacteriaceae is hexa-acyl, whereas non-enterobacteriaceae have a broader range of structures. by using a pr ... | 2009 | 19794055 |
| toll-like receptor signaling in airborne burkholderia thailandensis infection. | melioidosis is a tropical disease endemic in southeast asia and northern australia caused by the gram-negative soil saprophyte burkholderia pseudomallei. although infection is often systemic, the lung is frequently involved. b. thailandensis is a closely related organism that at high doses causes lethal pneumonia in mice. we examined the role of toll-like receptors (tlrs), essential components of innate immunity, in vitro and in vivo during murine b. thailandensis pneumonia. tlr2, tlr4, and tlr5 ... | 2009 | 19797072 |
| innate immune responses of pulmonary epithelial cells to burkholderia pseudomallei infection. | burkholderia pseudomallei, a facultative intracellular pathogen, causes systemic infection in humans with high mortality especially when infection occurs through an infectious aerosol. previous studies indicated that the epithelial cells in the lung are an active participant in host immunity. in this study, we aimed to investigate the innate immune responses of lung epithelial cells against b. pseudomallei. | 2009 | 19806192 |
| use of a low-dose steroid as an adjunct in the treatment, in mice, of severe sepsis caused by burkholderia pseudomallei. | human melioidosis caused by burkholderia pseudomallei is a severe septic disease that is associated with high mortality, even under appropriate antibiotic treatment. the therapeutic effects of low-dose hydrocortisone plus ceftazidime, and of ceftazidime alone, have recently been investigated in the treatment of acute, severe sepsis caused by b. pseudomallei, both in normal balb/c mice and in balb/c mice with streptozotocin-induced diabetes. the mice were infected and then treated intravenously, ... | 2009 | 19825285 |
| pip(2)-binding site in kir channels: definition by multiscale biomolecular simulations. | phosphatidylinositol bisphosphate (pip(2)) is an activator of mammalian inwardly rectifying potassium (kir) channels. multiscale simulations, via a sequential combination of coarse-grained and atomistic molecular dynamics, enabled exploration of the interactions of pip(2) molecules within the inner leaflet of a lipid bilayer membrane with possible binding sites on kir channels. three kir channel structures were investigated: x-ray structures of kirbac1.1 and of a kir3.1-kirbac1.3 chimera and a h ... | 2009 | 19839652 |
| identifying translation initiation sites in prokaryotes using support vector machine. | motivation: gene identification in genomes has been a fundamental and long-standing task in bioinformatics and computational biology. many computational methods have been developed to predict genes in prokaryote genomes by identifying translation initiation site (tis) in transcript data. however, the pseudo-tiss at the genome level make these methods suffer from a high number of false positive predictions. in addition, most of the existing tools use an unsupervised learning framework, whose pred ... | 2010 | 19840808 |
| lack of correlation of burkholderia pseudomallei quantities in blood, urine, sputum and pus. | we evaluated the correlation of burkholderia pseudomallei quantities in blood versus urine, sputum or pus. correlations between bacterial counts in blood and other samples were not found. it is likely that an initial seeding event to extracellular organs is followed by independent growth of b. pseudomallei, and that bacteria in the urine were not passively filtered from the bloodstream. | 2009 | 19842414 |
| pathema: a clade-specific bioinformatics resource center for pathogen research. | pathema (http://pathema.jcvi.org) is one of the eight bioinformatics resource centers (brcs) funded by the national institute of allergy and infectious disease (niaid) designed to serve as a core resource for the bio-defense and infectious disease research community. pathema strives to support basic research and accelerate scientific progress for understanding, detecting, diagnosing and treating an established set of six target niaid category a-c pathogens: category a priority pathogens; bacillu ... | 2010 | 19843611 |
| the clinical profile and predictors of mortality in patients with melioidosis in south india. | melioidosis is an underdiagnosed and underreported disease in india with protean clinical manifestations. mortality in this study population was 17%. the predominant risk factor for melioidosis was diabetes mellitus. multifocal disease was present in 66% and pulmonary involvement in 61% of patients. in a country like india where the conditions are conducive for endemic melioidosis and due to the clinical similarity of melioidosis to diseases like tuberculosis, it is essential for clinicians to h ... | 2010 | 19850605 |
| pahang melioidosis registry. | melioidosis has a high annual incidence and mortality rate in pahang, malaysia. we initiated the first melioidosis registry in the country on 1st july 2005 to improve the management of melioidosis in the state. continuous medical education on melioidosis was carried out in all hospitals in the state to highlight the magnitude of the disease and to educate the doctors on the treatment of the disease. all culture confirmed cases were registered and analysed. during the one-year study period from 1 ... | 2009 | 19852316 |