Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| in vivo incorporation of drosophila h2a histone into mammalian chromatin. | hybrid prokaryotic/eukaryotic expression vectors have been used to introduce drosophila histone genes into cv-1 african green monkey tissue culture cells. transfection of cv-1 cells with drosophila genes under the control of insect dna promoter sequences results in low level expression of histone genes. on the other hand, when the drosophila h2a gene is juxtaposed downstream from the long terminal repeat sequence of rous sarcoma virus (rsv) expression of the insect gene is considerably more effi ... | 1983 | 6406983 |
| antibodies to a defined region of pp60src neutralize the tyrosine-specific kinase activity. | site-specific antibodies to pp60src, the transforming protein of rous sarcoma virus (rsv), have been prepared by immunizing rabbits with a chemically synthesized pentadecapeptide corresponding to residues 498-512 (cys-trp-arg-lys-asp-pro-glu-glu-arg-pro-thr-phe-lys-tyr-leu) as deduced from the nucleotide sequence of the prague c src gene. antibodies specific for the synthetic peptide were purified from immune sera by affinity chromatography on peptide-bound sepharose and characterized by a numbe ... | 1983 | 6411728 |
| a tumor promoter stimulates phosphorylation on tyrosine. | the tumor promoter 12-o-tetradecanoyl-phorbol-13-acetate is mitogenic for normal chicken embryo fibroblasts and also causes these cells to express transiently many properties of cells transformed by rous sarcoma virus. since some mitogenic hormones stimulate a tyrosine-specific protein kinase activity, and since the transforming protein of rsv is a tyrosine-specific protein kinase, we have examined whether tpa also stimulates protein phosphorylation on tyrosine. we report here that tpa treatment ... | 1983 | 6414472 |
| the effect of the tumor promoter, phorbol myristate acetate (pma), on hyaluronic acid (ha) synthesis by chicken embryo fibroblasts. | treatment of chicken embryo fibroblasts (cef) with the tumor promoter, phorbol myristate acetate (pma), resulted in a rapid increase in their ability to synthesize the glycosaminoglycan, hyaluronic acid (ha), whereas the parent compound, phorbol, had no effect. cef cultures incubated with pma (100 ng/ml) for 6 h resulted in a 15-fold increase in ha synthetase activity compared with phorbol-treated control cultures. the incorporation of [3h]acetate into ha and chemical determination of this polym ... | 1983 | 6414833 |
| pp60c-src kinase is in chick and human embryonic tissues. | the normal cellular protein pp60c-src is a tyrosine-specific protein kinase that is homologous to the transforming protein of rous sarcoma virus (rsv) but its function is unknown. the expression of pp60c-src in chick and human embryonic tissues was monitored by the immune complex protein kinase assay, western transfer analysis, and immunocytochemical staining at the light microscope level. pp60c-src kinase was expressed in the head and trunk regions of the chick embryo at all stages of developme ... | 1984 | 6428952 |
| direct evidence that oncogenic tyrosine kinases and cyclic amp-dependent protein kinase have homologous atp-binding sites. | p60src, the transforming protein of rous sarcoma virus (rsv), is a protein kinase that has a strict specificity for tyrosine. the phosphorylation of cellular proteins by p60src (ref. 4) results in transformation. recently, barker and dayhoff discovered that residues 259-485 of p60src have 22% sequence identity with residues 33-258 of the catalytic subunit of cyclic amp-dependent protein kinase, an enzyme that has a specificity for serine. because it was necessary to introduce eight gaps to align ... | 1984 | 6431300 |
| characterization of a transformation-sensitive protein in the extracellular matrix of chicken embryo fibroblasts. | the extracellular matrix of cultured chicken embryo fibroblasts undergoes a number of modifications during the early stages of oncogenic transformation. one alteration is increased production of a small protein (mr approximately 21,000) which is transiently deposited in the matrix by transforming cells infected with la24, a temperature-sensitive mutant of rous sarcoma virus (rsv) (blenis, j., and hawkes, s.p. (1983) proc. natl. acad. sci. u.s.a. 80, 770-774). this protein is a major component of ... | 1984 | 6432799 |
| respiratory viruses and mycoplasma pneumoniae infections at the time of the acute exacerbation of chronic otitis media. | the present study was undertaken to ascertain whether or not patients with chronic otitis media are infected with viruses or mycoplasma at the time of sudden increase in otorrhea. from 26 patients with acute exacerbation of chronic otitis media, sera were collected at the time of sudden increase in otorrhea and three to four weeks later. these paired sera were examined for antibody titer to respiratory viruses (21 species) and mycoplasma pneumoniae. of them, influenza b virus and rsv infections ... | 1984 | 6442134 |
| long-term prospective study in children after respiratory syncytial virus infection. | we have prospectively evaluated for the past 8 years 29 children who were hospitalized during infancy with acute lower respiratory tract illness caused by respiratory syncytial virus (rsv). no differences in the prevalence of a family history of atopy or breast-feeding in these infants compared with controls were noted. however, a history of parental smoking was significantly associated with hospital admission for rsv lower respiratory tract disease. evidence of atopy, as defined by serum ige le ... | 1984 | 6470859 |
| changes in ganglioside metabolism during in vitro differentiation of quail embryo myoblasts. | the metabolism of gangliosides was studied during the in vitro differentiation of both normal quail myoblasts and myoblasts which have been transformed by a temperature-sensitive mutant of rous sarcoma virus (rsv). these transformed cells can be maintained undifferentiated if incubated at 35 degrees c, but they will differentiate when shifted to 41 degrees c. (d. montarras and m. y. fiszman (1983) j. biol. chem. 258, 3882-3888). the analysis of [14c]glucosamine-labeled gangliosides by two-dimens ... | 1984 | 6479448 |
| interaction of polymorphonuclear leukocytes and viruses in humans: adherence of polymorphonuclear leukocytes to respiratory syncytial virus-infected cells. | the nature of neutrophil-respiratory syncytial virus (rsv) interaction was investigated by assessing factors that influence neutrophil adherence to rsv-infected tissue culture monolayers. the adherence of neutrophils to infected cells was directly proportional to the degree of rsv replication as evidenced by infectious virus production, cytopathological changes, or viral antigen appearance. sixty-one percent of the neutrophils adhered to the rsv-infected cells as compared with 52.7% on noninfect ... | 1984 | 6481852 |
| a comparison of three vaccines against respiratory syncytial virus in calves. | an inactivated vaccine against respiratory syncytial virus (rsv) was compared with two live vaccines. the inactivated (gc) vaccine consisted of glutaraldehyde-fixed bovine nasal mucosa cells persistently infected with rsv and emulsified with oil adjuvant. the live vaccines were a modified virus (mv) derived from a bovine strain of rsv and a temperature-sensitive mutant (ts-1) derived from a human strain. the gc vaccine was inoculated subcutaneously into 12 calves and the live vaccines intramuscu ... | 1984 | 6501875 |
| cellular immune response to infection with respiratory syncytial virus and influence of breast-feeding on the response. | virus-specific lymphocyte transformation (ltf) activity in vitro was examined in 78 infants with various forms of illness due to respiratory syncytial virus (rsv) infection. in 73 subjects with lower respiratory tract disease, significant ltf activity was often detected within one week after onset of initial symptoms, and responses characteristic for each clinical form were observed in the subsequent rise. thus, mean activity in subjects with tracheobronchitis increased gradually, with the maxim ... | 1984 | 6512509 |
| prevalence of antibodies to respiratory syncytial virus in various populations in israel. | a preliminary study on the prevalence of antibodies to respiratory syncytial virus (rsv) in individuals of various age-groups was carried out. in healthy infants aged 6 to 12 months, the prevalence was 27%. it increased to 37 and 54% in children aged 1 to 2 and 2 to 4 years, respectively. the prevalence reached 78% in healthy individuals who were more than 20 years old, including pregnant women. significant levels of specific antibodies were detected in 59% of children aged 6 to 12 months who we ... | 1984 | 6519945 |
| an outbreak of respiratory syncytial virus pneumonia in a nursing home for the elderly. | an outbreak of pneumonia and febrile respiratory illness took place in a los angeles county nursing home for the elderly in february and march of 1979. forty of 101 (40%) residents were affected. twenty-two (55%) had pneumonia and eight (20%) died. the outbreak extended over a period of 6 weeks. serological evidence implicated respiratory syncytial virus (rsv) as the causative agent. three patients had fourfold or greater rises in the titre of antibody to rsv, one had high titres in paired sampl ... | 1984 | 6527041 |
| the development of respiratory syncytial virus-specific immune complexes in nasopharyngeal secretions following natural infection. | sequential samples of nasopharyngeal secretions (nps) from 61 infants and children with respiratory syncytial virus (rsv) infection were examined for the presence of rsv-specific immune complexes in order to determine their possible role in recovery from or pathogenesis of rsv infection. immune complexes in nps were identified by the raji cell assay using indirect immunofluorescence and rsv antigen in these complexes was also detected by indirect immunofluorescence. rsv-specific immune complexes ... | 1984 | 6527374 |
| a preliminary serological survey of viral antibodies in peruvian sheep. | this study reports the sero-prevalence of viral infections in sheep in peru. serum samples were collected from 34 mature healthy rams located in 3 different geographic regions of the country (north, central and south). the sera were tested for antibodies to the following viruses: respiratory syncytial virus (rsv); parainfluenza 3 (pi-3) virus; bovine viral diarrhea/border disease (bvd/bd) virus; bovine herpesvirus 1 (bhv-1); bluetongue (bt) virus; ovine progressive pneumoniae (opp) virus; bovine ... | 1984 | 6528458 |
| characterization of the human peripheral blood effector cells mediating antibody-dependent cell-mediated cytotoxicity against respiratory syncytial virus. | peripheral blood lymphocytes were separated into several subpopulations and evaluated for their ability to mediate antibody-dependent cell-mediated cytotoxicity (adcc) against respiratory syncytial virus (rsv)-infected hela cells. using erythrocyte rosetting methods, nylon wool filtration, and cytolysis with okt-3 monoclonal antibody, two lymphocyte subpopulations were shown to mediate rsv-adcc; non-t, non-b, and igg-fc receptor-bearing lymphocytes and e-rosetting cells with iggfc receptors (t g ... | 1983 | 6603313 |
| early release of the density-dependent inhibition of phosphate uptake and atp synthesis after src gene expression in chick embryo fibroblasts. | our results showed that the expression of the src gene in chick embryo fibroblasts (cef) released the density-dependent inhibition (ddi) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). with increasing cell density, phosphate metabolism decreased by 58% in normal cef and, in contrast, increased by 20% in rous sarcoma virus (rsv)-transformed cef. the same change in the ddi was observed in cef infected by ny68 (a ts mutant for transformation of rsv) and ma ... | 1984 | 6609830 |
| giant cell pneumonia due to respiratory syncytial virus. occurrence in severe combined immunodeficiency syndrome. | a 6-month-old male infant with a severe combined immunodeficiency syndrome was hospitalized for progressive respiratory distress. examination during hospitalization disclosed widespread pulmonary infiltrates that did not respond to intensive therapy. the patient died eight days after admission. autopsy disclosed pneumocystis carinii pneumonia and widespread giant cell pneumonia. respiratory syncytial virus (rsv) was grown from a lung specimen obtained at autopsy. specific immunofluorescent stain ... | 1984 | 6611144 |
| a study of respiratory infections in the elderly to assess the role of respiratory syncytial virus. | a prospective study was performed from 1 december 1981 to 31 may 1982 in two departments of geriatric medicine in edinburgh. this yielded 159 cases of acute respiratory tract infection (rti). twelve of these were undoubtedly associated with respiratory syncytial virus (rsv), 14 with influenza a and 18 with influenza b (as established in each case by a fourfold or greater increase in antibody titre). eighty-five of the 159 patients with rti and rsv titres of 32 or greater. their significance is d ... | 1983 | 6663083 |
| [regulatory proteins of the cytoskeleton system and their changes associated with carcinogenesis]. | microfilaments are known to participate in several cellular functions. proteins which cross-link, sever or cap f-actin filaments, and those inhibit polymerization of g-actin may regulate intracellular distribution, length and amount of f-actin to cope with wide varieties of cellular functions of microfilaments. actinogelin, a calcium-regulated crosslinking protein of f-actin, was discovered in and purified from ehrlich tumor cells. native molecule of the protein was found to be consisted of two ... | 1983 | 6683952 |
| bovine respiratory syncytial virus infections in young dairy cattle: clinical and haematological findings. | clinical signs and haematological findings during the acute and convalescent phases of an infection with bovine respiratory syncytial virus (rsv) were studied under field conditions. the study was carried out in 139 cattle less than 16 months of age in 16 herds with serologically proven bovine rsv infections. blood was collected for serological and haematological examination. repeated clinical examinations were carried out until 35 days after appearance of disease. signs of general disease such ... | 1984 | 6702077 |
| respiratory syncytial virus-related apnea in infants. demographics and outcome. | medical records of 261 hospitalized patients less than 1 year old with documented respiratory syncytial virus (rsv) infection were reviewed to determine the incidence of rsv-associated apnea and the accompanying risk of subsequent apnea or death. apnea in association with rsv infection occurred in 18% of the infants. premature birth and a young postnatal age were risk factors for development of apnea with rsv disease. apnea of prematurity appeared to be a significant risk factor for rsv apnea de ... | 1984 | 6702769 |
| purification and characterization of gp90, one of the envelope glycoproteins of respiratory syncytial virus. | the large glycoprotein, gp90, of respiratory syncytial virus (rsv) was purified by affinity chromatography using a monoclonal antibody. hyperimmune rabbit antiserum directed specifically to the purified gp90 neutralized rsv to high titre but did not inhibit fusion of previously infected cells. 125i-labelled gp90 bound rapidly to hep-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum. | 1984 | 6707612 |
| infection rates of respiratory syncytial virus in pediatric patients attending phra mongkutklao hospital, bangkok. | respiratory syncytial virus (rsv) and other pathogens were isolated from nasopharyngeal secretions from 200 pediatric patients attending the out patient department of phra mongkutklao hospital with symptoms of upper respiratory tract infections. their sera were also taken for determination of class specific immunoglobulin antibody titers. the positive isolation rates were 36% for rsv, 5.5% for adenovirus 1.5% for herpes simplex virus (hsv), and 4% for staphylococcus aureus. one to 5.5% of these ... | 1984 | 6740381 |
| virus-induced complement activation and neutrophil-mediated cytotoxicity against respiratory syncytial virus (rsv). | complement-dependent neutrophil-mediated cytotoxicity (cdnc) was determined by specific release of 51-chromium (51cr) from respiratory syncytial virus infected hep2 cells in a microcytotoxicity assay. there was significant release of 51cr from rsv infected cells as compared to uninfected cells in the presence of complement (c) and neutrophils (pmn). the degree of cytotoxicity was dependent upon the concentration of c used in the assay. such cytotoxicity was effectively abolished after heat-inact ... | 1984 | 6744659 |
| diagnosis of respiratory syncytial virus infection in children: comparison of viral antigen detection and serology. | direct detection of viral antigen in nasopharyngeal secretion by radioimmunoassay was compared with serology by igg antibody enzyme immunoassay for diagnostic efficacy in 77 children with clinically suspected respiratory syncytial virus (rsv) infections. antigen detection gave a positive diagnosis in 26 of 33 (79%) children in whom rsv infection was diagnosed by any of the two methods. the diagnostic efficacy of antigen detection was dependent upon the interval after onset at which specimens wer ... | 1984 | 6747612 |
| immunoglobulin class-specific antibody response in respiratory syncytial virus infection measured by enzyme immunoassay. | immunoglobulin class-specific enzyme immunoassay (eia) was used for determination of antibody responses in sera collected from 26 children with acute primary respiratory syncytial virus (rsv) infections. all 26 patients had igg antibody responses with a significant titer increase in 24 (92%); an igm antibody response was detected in 19 of the 26 (73%). from patients aged 6 months or less only 5 of 8 produced detectable igm antibodies, whereas all patients aged 1-2 years did so. igm antibodies ap ... | 1984 | 6747613 |
| enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description. | an indirect enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (rsv) antigens was developed, using commercially available antisera. horse anti-rsv and calf antiserum to bovine rsv were used as capture and detector antibodies, respectively. the assay could detect as few as 50 pfu of unpurified rsv per ml in infected cell culture supernatant fluids and as little as 10 ng of affinity-purified rsv antigen per ml. no cross-reactions were observed with heterologous virus ty ... | 1982 | 6749894 |
| respiratory syncytial virus: its transmission in the hospital environment. | respiratory syncytial virus (rsv) over the past two decades has been recognized as the most important cause of lower respiratory tract disease in infants and young children. recently, it has also been identified as a major nosocomial hazard on pediatric wards. the potential for rsv to spread on such wards is underlined by several singular characteristics of rsv. it arrives in yearly epidemics and is highly contagious in all age groups. immunity is of short duration, allowing repeated infections ... | 1982 | 6758370 |
| atopy does not predispose to rsv bronchiolitis or postbronchiolitic wheezing. | twenty-six 8-year-old children who had had respiratory syncytial virus (rsv) bronchiolitis in infancy and their paired controls underwent skin and blood tests to assess the role of immunodeficiency and atopy in the pathogenesis of rsv bronchiolitis and the wheezing that may follow it. there was no difference between patients and controls in prevalence of atopy; positive results of prick tests to common antigens; eosinophil counts; yeast opsonisation defect; c2 deficiency; igg, iga, igm, and ige ... | 1981 | 6788213 |
| respiratory infections in cystic fibrosis patients caused by virus, chlamydia and mycoplasma--possible synergism with pseudomonas aeruginosa. | 116 cystic fibrosis patients were observed, by monthly examinations over an eight-month period, to investigate the importance of non-bacterial respiratory infections (nbi) in exacerbations of the respiratory disease. sputum was examined for bacteria, and serum investigated for antibody response against virus, mycoplasma and chlamydia and for antibodies against pseudomonas aeruginosa. during this period each patient had, on an average, 2.9 exacerbations of which 76% were associated with bacteria, ... | 1981 | 6798822 |
| synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins. | all the known avian sarcoma viruses have associated protein kinase activities that phosphorylate tyrosine residues of their target proteins. a decapeptide fragment of pp60src of rous sarcoma virus (rsv), residues 415-424, and an analog of that sequence have been chemically synthesized by solid-phase methods. the two decapeptides were not phosphorylated by pp60src of rsv, p90 of y73 avian sarcoma virus, or p140 of fujinami sarcoma virus. however, both peptides were able to inhibit competitively t ... | 1981 | 6801651 |
| the expression in eukaryotes of a tyrosine kinase which is reactive with pp60v-src antibodies. | all specimens of eumetazoa and parazoa, ranging from mammals, birds, teleosts, sharks, lampreys, amphioxus, insects, down to sponges showed the pp60c-src associated kinase activity, indicating that c-src, which is the cellular homologue of the oncogene v-src of rous sarcoma virus (rsv) is probably present in all multicellular animals. protozoa and plants did not show pp60c-src kinase activity. the degree of c-src expression depends on the taxonomic rank of the eumetazoa tested, and is organ-spec ... | 1982 | 6819971 |
| identification of the virus-specific proteins of respiratory syncytial virus temperature-sensitive mutants by immunoprecipitation. | the proteins of long strain rsv and three temperature-sensitive (ts) mutants of the a2 strain were compared by pulse labeling virus-infected cells with [35s]methionine and [3h]glucosamine followed by analysis of the cell lysates by polyacrylamide gel electrophoresis. at the permissive temperature (30 degrees) proteins ranging in molecular weight from 24,000 to 50,000 (vp24, vp27, vp33, vp44) could be identified. immunoprecipitation of viral lysates by immune rabbit serum demonstrated antigenic s ... | 1983 | 6828465 |
| presumed respiratory syncytial virus pneumonia in three immunocompromised adults. | three cases of presumed respiratory syncytial virus (rsv) pneumonia in immunocompromised adults are described. two patients had symptoms of cough, fever, and malaise, following completion of a course of combination chemotherapy for the treatment of acute lymphoblastic leukemia. the third patient, a juvenile onset diabetic, developed similar symptoms while hospitalized for severe hyperglycemia. chest roentgenograms showed lower lobe infiltrates in both leukemic patients and a bilateral non-conflu ... | 1983 | 6846393 |
| respiratory syncytial virus infection in young hospitalized children. identification of risk patients and prevention of nosocomial spread by rapid diagnosis. | detection of rsv by immunofluorescence was used for rapid confirmation of the diagnosis in 89 infants during an epidemic. this made it possible to plan the care of the patients and use the personnel in an optimal way in order to maintain nosocomial infection at a low level. somatic and environmental risk factors connected with serious disease were also identified. the course of the disease was more severe in children with chronic illnesses or those who had had perinatal complications. allergy wa ... | 1983 | 6858684 |
| cell-mediated immune response to respiratory syncytial virus infection in owl monkeys. | the suitability of owl monkeys as experimental models to study the cellular immune response to respiratory syncytial virus (rsv) infection was examined. seronegative owl monkeys inoculated intranasally with rsv shed large quantities of virus and developed clinically evident upper respiratory disease. rsv infected monkeys had significant lymphoproliferative responses to rsv antigen by 4 weeks post-infection. in contrast, no positive blastogenic responses were elicited during the acute phase of il ... | 1983 | 6883807 |
| prevention of infections with respiratory syncytial virus: the hopes and hurdles ahead. | control of infections due to respiratory syncytial virus (rsv) by immunization poses special problems. first, the peak period of serious illness due to rsv is during the first few months of life, and thus a vaccine would have to be administered during the neonatal period. second, we understand little of the pathogenesis of and immunity to rsv disease in newborns, and an immune reaction may even play a role in the development of the lower respiratory tract disease seen in infancy. third, immunity ... | 1980 | 6997967 |
| [critical considerations on the laboratory research in infections from respiratory syncytial virus (rsv) (author's transl)]]. | 1980 | 7008714 | |
| respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay. | a simplified enzyme-linked immunosorbent assay (elisa) which utilized commercially available reagents was developed for respiratory syncytial virus (rsv)-specific immunoglobulin g. an analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in rsv antibodies. the elisa was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 rsv-infected adults. the elisa correlated ... | 1980 | 7012179 |
| comparison of fluorescent-antibody, neutralizing-antibody, and complement-enhanced neutralizing-antibody assays for detection of serum antibody to respiratory syncytial virus. | a comparison of three assays for the detection of serum antibody to respiratory syncytial virus (rsv) was carried out on 47 serum samples obtained sequentially from infants and young children with rsv infection. neutralizing-antibody (na) activity was determined by a semimicromethod of plaque reduction. complement-enhanced na activity was determined by the addition of guinea pig complement to na assays. rsv antibody responses in immunoglobulin g, immunoglobulin m, and immunoglobulin a classes we ... | 1981 | 7016915 |
| clinical and laboratory diagnosis of respiratory syncytial virus infection. | infection with rsv is one of the most common respiratory viral infections in childhood, particularly in infants under 1 year of age. the disease is characterized by epidemic proportions of infection which occur regularly in all parts of the world in the winter months. the spectrum of the clinical disease is quite variable, and the infection may present as bronchiolitis, pneumonia, croup, or exacerbation of pre-existing reactive airway disease. recent evidence has suggested that rsv infection may ... | 1981 | 7018832 |
| diagnosis of respiratory syncytial virus infection in the bovine respiratory tract by immunofluorescence. | direct staining of nasopharyngeal smears with hyperimmune bovine serum raised against respiratory syncytial virus (rsv) and conjugated with fluorescein isothyocyanate was compared with virus isolation from 14 experimentally infected animals. twenty-four out of 107 specimens examined were positive by the fluorescent antibody (fa) method and 21 out of 107 by virus isolation. most of the fa positives (81 per cent) were detected after the ninth day of infection whereas 80 per cent of virus isolation ... | 1981 | 7027594 |
| serum migration-inhibitory activity in infants with respiratory syncytial virus bronchiolitis. | using an unidirectional leucocyte migration-inhibition (lmi) assay, we investigated the migration-inhibitory activity (lif) in the sera of 25 infants who developed bronchiolitis following respiratory syncytial virus (rsv) infection. blood samples for serum-lif activity detection were obtained from patients the day after admission and two weeks later. the lmi assay revealed serum-lif activity in 17 (68%) blood samples taken during the acute phase of the disease. in contrast, no inhibition was fou ... | 1981 | 7030363 |
| production of antiserum to respiratory syncytial virus polypeptides: application in enzyme-linked immunosorbent assay. | by use of crossed immunoelectrophoresis techniques, respiratory syncytial (rs) virus-specific precipitates were produced between rs virus cellular antigen [solubilized in tris(hydroxymethyl)aminomethane-glycine buffer, ph 9] and antiserum raised in rabbits against semipurified rs virus. when these precipitates were employed as antigens for further immunizations in rabbits, antibodies (anti-rsv-precip.i) were produced which reacted with only one rs virus antigen when tested by the crossed immunoe ... | 1981 | 7031083 |
| parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. | a live respiratory syncytial virus (rsv) vaccine administered parenterally was evaluated for efficacy in a double-blind, placebo-controlled field trial in 510 children six to 47 months of age. among the 233 recipients of live rsv vaccine, 68 of 98 initially seronegative children (as determined by enzyme-linked immunosorbent assay) developed antibody to rsv; 25 of the 30 initially seronegative children who did not develop antibody were less than 12 months old, which suggested that very low levels ... | 1982 | 7037983 |
| maternal antibody and respiratory syncytial virus infection in infancy. | one hundred newborn infants were studied prospectively for 1 year for evidence of infection with respiratory syncytial virus (rsv). the indirect membrane fluorescence technique was used to determine specific antibody in sera. infection was shown in 29 cases. in 31 infants exposed to an rsv epidemic season, there was no evidence of infection. maternal antenatal sera were also tested, and wide range of igg antibody to rsv was found. mean titre of maternal igg antibody to rsv was significantly high ... | 1981 | 7038043 |
| experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: immunofluorescent and electron microscopic studies. | colostrum-deprived lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. multiple tissues were examined by immunofluorescence to localize viral and bacterial antigens, and lungs were examined by electron microscopy for cytopathologic changes. using immunofluorescence, viral antigen was detected only in the respiratory tract, mainly in the bronchial and bronchiolar epithelium and in the alveolar wall. lesser amounts of vir ... | 1982 | 7046531 |
| appearance of complement components and immunoglobulins on nasopharyngeal epithelial cells following naturally acquired infection with respiratory syncytial virus. | nasopharyngeal epithelial cells (npec) were collected from 144 infants and children with respiratory syncytial virus (rsv) infection, and were analyzed by fluorescent antibody techniques for the presence of cell-bound complement (c'3), iga, igg, and igm class of immunoglobulins (ig), and respiratory syncytial virus antigen. viral antigen was present on the surface of npec in 100% of samples obtained in the first 3 days or illness. the percentage of patients positive for rsv antigen declined stea ... | 1982 | 7069409 |
| respiratory syncytial viral infection in infants with congenital heart disease. | occasional reports have suggested that infants with congenital heart disease may have an increased risk of severe illness from respiratory syncytial virus (rsv) infection. we prospectively studied 699 infants hospitalized during the winters of 1976 through 1980, when rsv was prevalent in the community; 229 of these infants had proved rsv infections acquired either before admission or during hospitalization; 27 had both congenital heart disease and rsv infection, and 46 had congenital heart disea ... | 1982 | 7088112 |
| experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: pathologic studies. | nineteen 4-week-old, colostrum-deprived lambs were transtracheally inoculated with respiratory syncytial virus (rsv), pasteurella haemolytica biotype a serotype 1, or rsv and p haemolytica. pneumonic lesions were more frequent, more extensive, and more severe in lambs inoculated with rsv and p haemolytica than in lambs inoculated with either agent alone. lesions were seen in 2 of 4 lambs inoculated with p haemolytica alone, in 3 of 4 lambs inoculated with rsv alone, and in 11 of 11 lambs inocula ... | 1982 | 7091824 |
| experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: clinical and microbiologic studies. | four-week-old lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. when given in combination, rsv administration preceded p haemolytica by 3 or 5 days. lambs inoculated with p haemolytica or rsv developed a mild respiratory tract disease accompanied by a transient pyrexia in a few lambs. by 24 hours after inoculation of bacteria, all lambs inoculated with rsv and p haemolytica were listless, reluctant to move, and exhibi ... | 1982 | 7091825 |
| administration of a highly attenuated, live respiratory syncytial virus vaccine to adults and children. | a highly attenuated respiratory syncytial virus (rsv) experimental vaccine, rsv ts-2, was sequentially evaluated in adults, seropositive children, and finally, fully susceptible seronegative children. the vaccine was administered intranasally in doses ranging from 10(5.2) to 10(6.3) pfu/ml. in both adults and children, the vaccine proved to be poorly infectious. although poor infectivity would not have been predicted from tissue culture studies of rsv ts-2 growth, the human experience closely pa ... | 1982 | 7107009 |
| the possible role of respiratory syncytial virus and pasteurella spp in calf respiratory disease. | in a natural outbreak of respiratory disease during 1976, 31 of 43 calves showed moderately severe clinical signs which included pyrexia (maximum 42 degrees c) and tachypnoea. during the outbreak infection by respiratory syncytial virus (rsv) was demonstrated by isolation from nasopharyngeal swabs or by serology. pasteurella haemolytica or p multocida were isolated from the blood of four and five calves respectively. in the month before disease 65 per cent of the calves showed significant antibo ... | 1980 | 7210428 |
| respiratory syncytial virus infections in oslo 1972--1978. i. virological and epidemiological studies. | respiratory syncytial virus (rsv) infection was diagnosed in 464 children admitted to hospital with acute respiratory disease from autumn 1972 to spring 1978. the virological surveillance was approximately 70%. as a rule, rsv infections occurred in distinct outbreaks in late autumn and winter together with a marked increase in the number of children admitted to hospital with acute lower respiratory tract disease. the incidence in children less than 1 year of age was about 10/1000 children/year. ... | 1980 | 7211356 |
| respiratory syncytial virus infections in oslo 1972--1978. ii. clinical and laboratory studies. | patient records from 422 children admitted to hospital during 6 winter outbreaks of rsv infection were examined retrospectively. bronchiolitis (255 patients) and pneumonia (98 patients) were the most common diagnoses. otitis media (76 patients) occurred as a complication to these conditions. children at risk for contracting severe illness caused by rsv were infants below the age of one year, more often boys than girls. of the children older than two years, 32% had already been diagnosed as asthm ... | 1980 | 7211357 |
| nosocomial respiratory syncytial viral infections. should gowns and masks be used? | the efficacy of infection control procedures utilizing gowns and masks in the control of nosocomial respiratory syncytial virus (rsv) infections was evaluated by comparing the rate of nosocomial rsv infections in infants and ward personnel during two sequential periods when gowns and masks were used (period 1) and not used (period 2). all patients (162) and staff (36) on our infants' ward were examined for signs of respiratory infection and had nasal washes obtained for viral isolation every two ... | 1981 | 7234784 |
| [immunological studies of the relation between respiratory syncytial virus (rsv) and bronchiolitis]. | 1981 | 7242476 | |
| legionnaires' disease combined with erythema multiforme in a 3-year-old boy. | a case of legionnaires' disease (ld) is described in a 3-year-old boy. he had fulminant disease with typical signs like bilateral pneumonia, gastrointestinal symptoms, and somnolence indicating involvement of the central nervous system. there was no premorbidity. an outstanding development was erythema multiforme, which has never previously been described in ld. the basic disease was caused by legionella pneumophila. this is evidenced by specific serum igm at admission and a subsequent significa ... | 1981 | 7246139 |
| [prospects of respiratory syncytial virus vaccines]. | the latest vaccines for respiratory syncytial virus (rsv) are reviewed. a ts-1 variant is being studied in the attempt to complete the attenuation of the vaccine strain. further investigations of live rsv vaccine administered parenterally indicate that a target population over 6 months of age could benefit soon of this preparation. | 1980 | 7247482 |
| effect of respiratory syncytial virus and virus-antibody complexes on the oxidative metabolism of human neutrophils. | the effect of respiratory syncytial virus (rsv) or mixtures of rsv and its specific antibody on the oxidative metabolic activity of human polymorphonuclear leukocytes was studied by the technique of luminol-dependent chemiluminescence. peripheral blood neutrophils obtained from normal healthy donors were used. rsv alone failed to induce any chemiluminescent response by the neutrophils. however, mixtures of rsv and rsv antibody-positive serum regularly elicited significant neutrophil chemilumines ... | 1981 | 7251140 |
| bronchomammary axis in the immune response to respiratory syncytial virus. | the products of lactation from 26 nursing mothers were sequentially examined over several months for the presence or appearance of antibodies directed against respiratory syncytial virus. antiviral igm and igg were rarely identified in either colostrum or milk. rsv-specific iga was found in 75% (18/24) of specimens of colostrum; 40% (6/15) and 59% (4/7) of milk samples obtained at three and six months still contained specific iga antibody. the latter increase was felt to represent boosting of ex ... | 1981 | 7252673 |
| activation of complement by cells infected with respiratory syncytial virus. | the ability of respiratory syncytial virus (rsv)-infected he(p)-2 cells in culture to activate complement was investigated. after incubation of cells with various complement sources and buffer, binding of c3b to surfaces of infected cells was demonstrated by immunofluorescence with a double-staining technique. nonsyncytial and syncytial (i.e., fused, multinucleated) cells were separately enumerated. also, lysis of rsv-infected cells was assessed by lactic dehydrogenase release. in this system on ... | 1981 | 7263071 |
| secretory antibody response to respiratory syncytial virus infection. | the secretory antibody response to primary and secondary infection with respiratory syncytial virus (rsv) in igg, igm and 11s iga immunoglobulin classes was determined using an indirect immunofluorescence antibody technique. nasopharyngeal secretions were collected after naturally acquired primary or secondary infection with rsv. immunoglobulin a responses to rsv were significantly greater in infants older than 6 months of age at the time of infection than in younger infants. reinfection with rs ... | 1981 | 7294005 |
| correlation of proliferation of lung epithelium with intramuscular sensitization and complement-fixing antibody to respiratory syncytial virus in the golden hamster. | intramuscular sensitization of hamsters with several forms of respiratory syncytial virus (rsv) caused proliferation of lung epithelium. in contrast, intranasal injection of live virus rarely resulted in this phenomenon. a correlation existed between proliferation of lung epithelium and presence of complement-fixing antibody, but not between lung disease and delayed skin reactions. complement-fixing antibody to rsv was found to be independent of the influence of the thymus. | 1981 | 7337963 |
| possible transmission by fomites of respiratory syncytial virus. | to test whether nosocomial spread of respiratory syncytial virus (rsv) could occur through contact with environmental surfaces contaminated by rsv-infected nasal secretions, survival in the environment of rsv isolated from media, pooled adult secretions, and secretions from hospitalized infants was examined. rsv in freshly obtained infant secretions was recovered from countertops for up to 6 hr, from rubber gloves for up to 1 1/2 hr, from cloth gowns and paper tissue for 30--45 min, and from ski ... | 1980 | 7365274 |
| the antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. | serum antibody responses to primary and secondary infections with respiratory syncytial virus were determined in 67 hospitalized infants. responses in igg, igm, and iga immunoglobulin fractions were assayed using an indirect immunofluorescent technique. infection was confirmed by identification of rsv antigen in nasopharyngeal secretions using indirect immunofluorescence, and by recovery of the virus in tissue culture. igm specific antibody response was commonly observed within a few days of ons ... | 1980 | 7365579 |
| morphogenesis and structure of caprine respiratory syncytial virus. | cell cultures inoculated with caprine respiratory syncytial virus (rsv) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. small syncytia were seen after 36 hours in culture. after 48 hours in culture, syncytia were large and numerous and pleomorphic cytoplasmic inclusions were seen. these inclusions were more pronounced and numerous later in the infection cycle. indirect immunofluorescence revealed a diffuse to granular cytop ... | 1980 | 7416970 |
| the appearance of cell-bound ige in respiratory-tract epithelium after respiratory-syncytial-virus infection. | we studied the appearance of ige in the respiratory tract in 42 infants and young children with various forms of respiratory illness after infection by respiratory syncytial virus (rsv). ige was bound to exfoliated nasopharyngeal epithelial cells in most patients with rsv infection during the acute phase of infection, regardless of the form of illness. however, the continued presence of cell-bound ige was more common in patients with rsv-induced bronchiolitis or asthma than in patients with mild ... | 1980 | 7421946 |
| [association between viral infection and pleuropericarditis: study of a case list of pleurisy and pericarditis]. | the authors report a study on a group of 47 patients affected by serositis (pleuritis, pericarditis, pleuro-pericarditis) of suspected viral origin. two serum samples were taken from each patient at different times and were tested with many viral antigens by the complement fixation technique. the discussion is carried out on the lines followed by the majority of people in giving importance to their viral etiology and the viruses involved as agents of serositis are reported. in the present study ... | 1980 | 7459017 |
| role of t-lymphocyte subsets in recovery from respiratory syncytial virus infection in calves. | the role of t-cell subsets in respiratory syncytial virus (rsv) infection was investigated by using monoclonal antibodies (mabs) to selectively deplete gnotobiotic calves of cd4+, cd8+, or wc1+ gamma delta t-cell receptor+ lymphocytes. injection of these mabs produced specific reductions of the target cell populations in the circulation and tissues. ten days after rsv infection, immunoglobulin m (igm), igg1, and iga antibodies were detected in sera and lung washings from control calves. depletio ... | 1995 | 7474075 |
| a leucine triplet repeat sequence (lxx)4 in p6gag is important for vpr incorporation into human immunodeficiency virus type 1 particles. | incorporation of vpr into human immunodeficiency virus type 1 (hiv-1) virions is mediated by the gag protein, independently of other viral components. we have coexpressed vpr and gag constructs in a vaccinia virus expression system in order to map the region of gag involved in vpr packaging. deletion of the carboxyl-terminal p6 region of gag impaired the ability of gag to package vpr. to confirm the role of p6 in vpr packaging, rous sarcoma virus (rsv)-hiv chimeras containing hiv-1 p6 were const ... | 1995 | 7474102 |
| update: respiratory syncytial virus activity--united states, 1995-96 season. | respiratory syncytial virus (rsv), a common cause of winter outbreaks of acute respiratory disease, is associated each year with an estimated 90,000 hospitalizations and 4500 deaths from lower respiratory tract disease in both infants and young children in the united states (1). outbreaks occur annually throughout the united states, and community activity usually peaks within 1 month of the national peak in january or february (figure 1) (2). rsv activity in the united states is monitored by the ... | 1995 | 7476847 |
| development of cellular resistance to pp60v-src kinase-induced cell death. | the v-src gene of rous sarcoma virus (rsv) encodes pp60v-src, a tyrosine kinase that can initiate cellular transformation. high levels of v-src gene expression can either be cytotoxic or the cause of altered expression of cellular genes. examination of cytotoxic thresholds is difficult because cells expressing high levels of a cytotoxic oncogene will die. to evaluate quantitatively the cytotoxicity of pp60v-src on growth, we amplified two different v-src genes, under the control of the human hsp ... | 1995 | 7478570 |
| cold-passaged, temperature-sensitive mutants of human respiratory syncytial virus (rsv) are highly attenuated, immunogenic, and protective in seronegative chimpanzees, even when rsv antibodies are infused shortly before immunization. | a cold-passaged (cp) temperature-sensitive (ts) rsv mutant, designated rsv cpts-530, which possesses host-range mutations acquired during 52 passages at low temperature in bovine tissue culture and one or more ts mutations induced by chemical mutagenesis (shut-off temperature 39 degrees c) was found previously to be tenfold restricted in its replication in mice as compared to wild-type virus and stable genetically in nude mice. in the current study, we introduced additional attenuating mutations ... | 1995 | 7483808 |
| evaluation of the antiviral activity of n-(phosphonoacetyl)-l-aspartate against paramyxoviruses in tissue culture and against respiratory syncytial virus in cotton rats. | n-(phosphonoacetyl)-l-aspartate (pala), a potent inhibitor of l-aspartic acid transcarbamoylase, was evaluated for cytotoxicity and antiviral activity against three different paramyxoviruses in tissue culture, and for antiviral efficacy and toxicity in vivo using a cotton rat-respiratory syncytial virus (rsv) model. significant in vitro cytotoxicity was observed in proliferating cultures of hep-2 (ic50 = 250 micrograms/ml) and vero cells (ic50 = 32 micrograms/ml), but was less evident in culture ... | 1995 | 7486959 |
| bovine respiratory syncytial virus antibodies in non-bovine species. | to study the role of non-bovine species in the epidemiology of bovine respiratory syncytial virus (rsv) infections, sera obtained from 9 non-bovine animal species and from humans were examined for bovine rsv specific antibodies. sera were mainly from animals and humans which had been in contact with cattle. forty sera of each species were tested in an rsv specific whole virus elisa as well as in a peptide based elisa, that was developed to measure antibodies specific for bovine rsv. antibodies d ... | 1995 | 7487487 |
| serological response to respiratory syncytial virus infection in pediatric patients with a comparison to immunofluorescence and virus isolation. | the serological response to respiratory syncytial virus (rsv) in 125 pediatric patients hospitalized with acute lower respiratory infection was investigated by enzyme linked immunosorbent assay (elisa) for specific immunoglobulin (ig) a, igg, and igm and complement fixation (cf) test. by elisa, a 4-fold rise in igg titre in paired sera was most commonly found, followed by a rise in iga and igm titres. investigation by elisa and cf leads to the suggestion that major cf activity against rsv antige ... | 1995 | 7488342 |
| human atrial natriuretic peptide gene delivery reduces blood pressure in hypertensive rats. | chronic infusion of atrial natriuretic peptide (anp) has been shown to cause natriuresis, diuresis, and hypotension in rats and humans. we explored the effect of a continuous supply of anp by somatic anp delivery on genetically hypertensive rats. a dna construct containing the human anp gene fused to the rous sarcoma virus 3'-long terminal repeat (rsv-ltr) was injected intravenously into spontaneously hypertensive rats (shr) through the tail vein. expression of human anp in shr was identified in ... | 1995 | 7490138 |
| phosphorylation of ser232 directly regulates the transcriptional activity of the p protein of human respiratory syncytial virus: phosphorylation of ser237 may play an accessory role. | the phosphoprotein p of human respiratory syncytial virus (rsv) was expressed in eukaryotic cells in phosphorylated form. site-directed mutagenesis of the recombinant protein established ser232 as the major site of phosphorylation in vivo. phosphorylation of bacterially made p protein in vitro by purified casein kinase ii (ckii) resulted in the phosphorylation of ser237, whereas mainly ser232 was phosphorylated by a crude cell extract. the p kinase activity in the cell extract exhibited properti ... | 1995 | 7491765 |
| safety and immunogenicity of the pfp vaccine against respiratory syncytial virus (rsv): the western blot assay aids in distinguishing immune responses of the pfp vaccine from rsv infection. | pfp-1 vaccine was evaluated in a randomized, controlled study in 47 rsv seropositive children. trivalent inactivated influenza virus (tiv) vaccine was the control. vaccine reactions were monitored, and bloods were obtained before vaccination, 4 weeks after vaccination, and at the end of the rsv season. respiratory illnesses were evaluated during the outbreak. neutralizing antibody (nt ab) assay to rsv, igg elisa to rsv proteins and a western blot assay were performed. acute reactions with the pf ... | 1995 | 7491817 |
| respiratory syncytial virus--associated lower respiratory diseases in hospitalised pre-school children in ibadan. | an analysis of the features of lower respiratory diseases (lrd) associated with respiratory syncytial virus (rsv) in 21 children aged 1-23 months, indicated that the clinical features did not differ appreciably from those described for lrd due to other infective agents. it was however, remarkable that none of the children was grossly malnourished, neither was there any death. the final respiratory diagnoses were bronchiolitis alone (6 cases), bronchopneumonia (6 cases), empyema/pleural effusion ... | 1995 | 7495200 |
| respiratory syncytial virus in infants and children. | respiratory syncytial virus (rsv) is a frequent cause of respiratory illness in infants and children, affecting almost 100% of children by the age of 3 years. mild rsv infection usually involves only the upper respiratory tract, while the lower respiratory tract is involved in severe disease. this article addresses the epidemiology of rsv infection, transmission of the disease, predisposing conditions, clinical course, diagnosis, and management. the majority of children can be managed on an outp ... | 1995 | 7501313 |
| alteration of cytokine secretion and cytotoxicity of murine alveolar macrophages after in vitro infection with respiratory syncytial virus (rsv). | 1995 | 7502897 | |
| ribozyme cleaves rex/tax mrna and inhibits bovine leukemia virus expression. | bovine leukemia virus (blv) encodes at least two regulatory proteins, rex and tax. tax, the transactivating protein, stimulates the long terminal repeat to promote viral transcription and may be involved in tumorigenesis. rex is involved in the transition from early expression of regulatory proteins to later expression of viral structural proteins. we have targeted ribozymes against the mrna encoding rex and tax. the ribozymes consist of the hammer-head catalytic motif flanked by antisense seque ... | 1993 | 7504287 |
| enhanced pulmonary pathology associated with the use of formalin-inactivated respiratory syncytial virus vaccine in cotton rats is not a unique viral phenomenon. | the specificity of viral antigens in the formalin-inactivated, alum-precipitated respiratory syncytial virus (fi-rsv) vaccine in augmenting the pulmonary inflammatory response was evaluated. cotton rats were immunized with a fi-rsv vaccine derived from vero cells, a monkey cell line, or hep-2 cells, a human cell line. the fi-rsv/vero and the fi-rsv/hep-2 vaccines were prepared similarly to the original lot-100 fi-rsv vaccine that was associated with enhanced disease in the mid-1960s field trials ... | 1993 | 7508665 |
| rous sarcoma virus-transformed cells develop peculiar adhesive structures along the cell periphery. | alteration of the cell/substratum adhesive structures of rat fibroblasts (3y1 cells) upon transformation by rous sarcoma virus (rsv) was investigated by immunofluorescence microscopy. in serum-containing culture medium, 3y1 cells developed focal adhesions as their main adhesive structures, while by1 cells expressed peculiar close contacts along the cell periphery with the vitronectin receptor integrin, in addition to podosomes. these peripheral close contacts are referred to as the peripheral ad ... | 1993 | 7510299 |
| preparation of a monoclonal antibody and expression of its antigen associated with myogenic differentiation on spontaneous and artificial myotubes derived from avian myoblasts. | quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus (ts-rsv) proliferate and do not differentiate at 35.5 degrees c, the permissive temperature for the virus, whereas their myoblast differentiation proceeds at 41.0 degrees c, a non-permissive temperature. in this experimental system, myogenic differentiation is controlled by src gene products. using qm-rsv cells as an antigen, a monoclonal antibody, mb-n3, was prepared. expression of mb-n3 antigen was found to i ... | 1993 | 7513264 |
| respiratory syncytial virus nucleocapsid protein (n) expressed in insect cells forms nucleocapsid-like structures. | the gene coding for the n protein of rsv strain long has been cloned and sequenced. it was introduced behind the polyhedrin promoter of the shuttle vector pvl941 and baculoviruses containing the n gene were constructed by homologous recombination. infection of spodoptera frugiperda 9 cells resulted in the production of large amounts of a protein similar in size and antigenicity to the authentic n protein. the baculovirus expressed n protein was concentrated in the cytoplasm of the insect cells a ... | 1994 | 7513931 |
| interleukin-8, interleukin-6, and soluble tumour necrosis factor receptor type i release from a human pulmonary epithelial cell line (a549) exposed to respiratory syncytial virus. | the release of interleukin-8 (il-8), interleukin-6 (il-6) and the soluble forms of the tumour necrosis factor receptor (stnf-r) from human pulmonary type ii-like epithelial cells (a549) after respiratory syncytial virus (rsv) infection was analysed. rsv infection alone induced a time- and rsv dose-dependent il-8 and il-6 release from a549 cells. furthermore, the soluble form of the tnf-ri was also secreted in a time- and rsv dose-dependent fashion. the soluble tnf-rii was not detected in the cel ... | 1994 | 7519169 |
| direct in vivo gene transfer and expression in malignant cells using adenovirus vectors. | to evaluate the ability of replication-deficient, recombinant adenovirus vectors to transfer genes to human tumor cells in vivo, adenovirus vectors containing the escherichia coli lacz (ad.rsv beta gal) gene (coding for beta-galactosidase; used as a cell marker for gene transfer) or the human alpha 1-antitrypsin (ad-alpha 1at) cdna (used as an example of a secreted protein) were administered intraperitoneally to nude mice with human malignant mesothelioma cell (h-meso-1) malignant ascites. preli ... | 1994 | 7519451 |
| potassium single-channel properties in normal and rous sarcoma virus-transformed chicken embryo fibroblasts. | ion channels in normal and rous sarcoma virus (rsv)-transformed chicken embryo fibroblasts (cefs) were examined by using the patch-clamp technique. three different types of ion channels were observed with single-channel conductances in symmetrical 140 mm kcl (with frequencies of occurrence in parentheses) of 186 ps (70%), 110 ps (10%), and 65 ps (20%), which are identical in normal and rsv-transformed cefs. the total channel density in both cell types is about 0.13 per micron2. all three types o ... | 1994 | 7520157 |
| respiratory syncytial virus infections in malnourished children. | the prevalence of respiratory syncytial virus (rsv) infection among severely malnourished children was studied at the university of benin teaching hospital, benin city, nigeria at a time when the infection was known to be prevalent in the community. nasopharyngeal washings were obtained from subjects on admission and thereafter every 4 days until discharge. rsv was detected by elisa technique. of 20 well nourished children who served as controls, 11 were elisa-positive for rsv (55%). eight (16%) ... | 1994 | 7521627 |
| linear antigenic and immunogenic regions of human respiratory syncytial virus n protein. | three linear antigenic regions on the n protein from human respiratory syncytial virus (rsv) subgroup a (strain a2) were identified by using peptides which reacted in elisa with sera from humans with recent or previous rsv infection. the determinants were localized within three hydrophilic regions of the protein: thr11 to gly30 (n3 peptide), ser231 to ala250 (n25 peptide) and thr371 to leu391 (n39 peptide). the site represented by the n39 peptide reacted with four subgroup a-specific mabs. there ... | 1995 | 7531217 |
| preparation of respiratory syncytial virus subgroup a and b antigens for enzyme immunoassay antibody detection. | a simplified method was described for purification of respiratory syncytial virus (rsv) subgroup a and b aimed to be used as antigens in enzyme immunoassay (eia). the titer of each rsv subgroup and the amount of protein was determined from the visible band in 45% sucrose gradient. the quality of prepared rsv subgroup antigens for eia was described in terms of the achievable final titer, the amount of protein, and eia criss-cross titration. the rsv subgroup a and b antigens, diluted as 1:100 (low ... | 1994 | 7532051 |
| [some characteristics of circulation and respiratory viruses in the country]. | the 17-year observation of changes in the etiological pattern of acute respiratory diseases (ard) in the country has demonstrated that different types of adenoviruses are prevalent in the circulation on the whole territory. adenoviral infection was found to prevail among non-influenza infections in the ukraine, rsv infection in the north of the russian federation and in the far east; piconaviral infections caused by echo and coxsackievirus b in the urals and byelarus republic. however, some year ... | 1994 | 7532499 |
| molecular epidemiology of respiratory syncytial virus: a review of the use of reverse transcription-polymerase chain reaction in the analysis of genetic variability. | respiratory syncytial virus (rsv) is the major viral cause of lower respiratory tract disease (bronchiolitis and pneumonia) in babies and infants. infections with the virus occur as annual winter epidemics in temperate climates, placing considerable pressure on the provision of hospital beds. the virus is unusual in that it can reinfect individuals and it can infect babies despite the presence of maternal antibody. rsv has a negative sense nonsegmented rna genome and as such is liable to high le ... | 1995 | 7541747 |
| antigenic analysis of the f protein of the bovine respiratory syncytial virus: identification of two distinct antigenic sites involved in fusion inhibition. | from two independent fusions, fifteen mabs directed to the f protein of the bovine respiratory syncytial virus (brsv) were characterized by radio-immunoprecipitation assays. competition binding assays among these mabs identified two distinct antigenic sites (a and b) and one overlapping site (ab). all of the mabs specific to epitopes belonging to site a neutralized the infectivity of the virus in vitro and recognized human and bovine rsv strains. only two out of the five mabs directed to epitope ... | 1995 | 7541983 |