Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| blood collected on filter paper for wildlife serology: evaluating storage and temperature challenges of field collections. | filter-paper (fp) blood sampling can facilitate wildlife research and expand disease surveillance. previous work indicated that nobuto fp samples from caribou and reindeer (rangifer tarandus subspecies) had comparable sensitivity and specificity to serum samples (≥ 80% for both) in competitive enzyme-linked immunosorbent assays (celisas) for brucella spp., neospora caninum, and west nile virus. the same sensitivity and specificity criteria were met in indirect elisas for brucella spp., bovine he ... | 2014 | 24499329 |
| mortality of live export cattle on long-haul voyages: pathologic changes and pathogens. | the cause of death in 215 cattle on 20 long-haul live export voyages from australia to the middle east, russia, and china was investigated between 2010 and 2012 using gross, histologic, and/or molecular pathology techniques. a quantitative reverse transcription polymerase chain reaction (qrt-pcr) assay was used to detect nucleic acids from viruses and bacteria known to be associated with respiratory disease in cattle: bovine coronavirus (betacoronavirus 1), bovine herpesvirus 1, bovine viral dia ... | 2014 | 24518275 |
| three viruses of the bovine respiratory disease complex apply different strategies to initiate infection. | bovine respiratory disease complex (brdc) is the major cause of serious respiratory tract infections in calves. the disease is multifactorial, with either stress or reduced immunity allowing several pathogens to emerge. we investigated the susceptibility of bovine airway epithelial cells (baec) to infection by the three major viruses associated with the brdc: bovine respiratory syncytial virus (brsv), bovine herpesvirus type 1 (bhv-1) and bovine parainfluenza virus type 3 (bpiv3). for this purpo ... | 2014 | 24548739 |
| population-based incidence of severe acute respiratory virus infections among children aged <5 years in rural bangladesh, june-october 2010. | better understanding the etiology-specific incidence of severe acute respiratory infections (saris) in resource-poor, rural settings will help further develop and prioritize prevention strategies. to address this gap in knowledge, we conducted a longitudinal study to estimate the incidence of saris among children in rural bangladesh. | 2014 | 24587163 |
| viral etiology of influenza-like illnesses in huizhou, china, from 2011 to 2013. | little information is available on the etiology and prevalence of viruses other than influenza viruses causing influenza-like illnesses (ilis) in china. this study was conducted for simultaneous detection and identification of 14 respiratory viruses in huizhou using real-time pcr. in total, viruses were detected in 48.66 % of ili patient samples, in which influenza virus (19.98 %) was the most commonly detected, followed by rhinovirus (7.46 %), human coronaviruses (3.63 %), human metapneumovirus ... | 2014 | 24610554 |
| infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex. | bovine respiratory syncytial virus (brsv), bovine parainfluenza virus type 3 (bpiv3) and bovine herpesvirus type 1 (bhv-1) are important pathogens associated with the bovine respiratory disease complex (brdc). non-bovine ruminants such as goats may also be infected and serve as a virus reservoir to be considered in the development of control strategies. to evaluate the susceptibility of caprine airway epithelial cells to infection by viruses of brdc, we established a culture system for different ... | 2014 | 24612951 |
| respiratory virus infections among children in south china. | acute respiratory tract infection is an important cause of morbidity and mortality with a worldwide disease burden. this study aimed to determine the prevalence and clinical characteristics of children with viral-induced acute respiratory tract infection, in southern china. nasopharyngeal aspirate samples from 1,980 pediatric patients with suspected acute respiratory tract infection, and 82 samples from healthy subject controls were collected for routine examination at the second affiliated hosp ... | 2014 | 24619492 |
| pathogenesis of a genotype c strain of bovine parainfluenza virus type 3 infection in albino guinea pigs. | bovine parainfluenza virus type 3 (bpiv3) is one of the most important of the known viral respiratory tract agents of both young and adult cattle and widespread among cattle around the world. up to present, three genotypes a, b and c of bpiv3 have been described on the basis of genetic and phylogenetic analysis and only limited studies on the pathogenesis of the genotype a of bpiv3 infection in calves and laboratory animals have been performed. the report about experimental infections of the gen ... | 2014 | 24681303 |
| comparison of levels and duration of detection of antibodies to bovine viral diarrhea virus 1, bovine viral diarrhea virus 2, bovine respiratory syncytial virus, bovine herpesvirus 1, and bovine parainfluenza virus 3 in calves fed maternal colostrum or a colostrum-replacement product. | colostrum-replacement products are an alternative to provide passive immunity to neonatal calves; however, their ability to provide adequate levels of antibodies recognizing respiratory viruses has not been described. the objective of this study was to compare the serum levels of igg at 2 d of age and the duration of detection of antibodies to bovine viral diarrhea virus 1 (bvdv-1), bovine viral diarrhea virus 2 (bvdv-2), bovine respiratory syncytial virus (brsv), bovine herpesvirus 1 (bhv-1), a ... | 2014 | 24688168 |
| [serological survey of antibodies against viral diseases of public health interest in llamas (lama glama) from jujuy province, argentina]. | llama population from argentina is mainly concentrated in the andean puna, jujuy. llamas represent an important economic resource for the andean communities. the aim of this study was to investigate the prevalence of antibodies against viral antigens associated to viral diseases of economic impact (neonatal diarrhea, reproductive and respiratory syndromes). a total of 349 serum samples from adult llamas were analyzed. the obtained antibody prevalence was 100 % for rotavirus a and 70 % for bovine ... | 2014 | 24721276 |
| intranasal delivery of nanoparticles encapsulating bpi3v proteins induces an early humoral immune response in mice. | vaccine adjuvants are typically designed to stimulate both systemic and mucosal immune responses. polymeric nanoparticles have been used as adjuvants in the development of vaccines against a number of viral pathogens and tested in laboratory animals. the objective of the study was to assess if synthetic bovine parainfluenza virus type-3 (bpi3v) peptide motifs and solubilised bpi3v proteins encapsulated in poly (dl-lactic-co-glycolide) (plga) nanoparticles (nps) induce specific humoral immune res ... | 2014 | 24726021 |
| [viral etiology in children with acute lower respiratory tract infections plus platelet disorders in changsha, china: an analysis of 255 cases]. | to investigate the viral etiology in hospitalized children with acute lower respiratory tract infections (alrti) plus platelet disorders. | 2014 | 24750840 |
| respiratory viruses in exacerbations of non-cystic fibrosis bronchiectasis in children. | respiratory viral infections precipitate exacerbations of chronic respiratory diseases such as asthma and chronic obstructive pulmonary disease though similar data in non-cystic fibrosis (cf) bronchiectasis are missing. our study aimed to determine the point prevalence of viruses associated with exacerbations and evaluate clinical and investigational differences between virus-positive and -negative exacerbations in children with bronchiectasis. | 2014 | 24819370 |
| the p value of hpiv3-mediated autophagy inhibition. | autophagy, a vesicular pathway involving the lysosomal degradation of cytosolic contents, is frequently hijacked by intracellular pathogens to optimize their infectivity. in this issue of cell host & microbe, ding et al. (2014) show that human parainfluenza virus exploits autophagy by targeting snares and preventing the snap29-stx17 interaction, an otherwise crucial event in autophagosome-lysosome fusion. | 2014 | 24832445 |
| phosphoprotein of human parainfluenza virus type 3 blocks autophagosome-lysosome fusion to increase virus production. | autophagy is a multistep process in which cytoplasmic components, including invading pathogens, are captured by autophagosomes that subsequently fuse with degradative lysosomes. negative-strand rna viruses, including paramyxoviruses, have been shown to alter autophagy, but the molecular mechanisms remain largely unknown. we demonstrate that human parainfluenza virus type 3 (hpiv3) induces incomplete autophagy by blocking autophagosome-lysosome fusion, resulting in increased virus production. the ... | 2014 | 24832451 |
| wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 for generation and characterization of monoclonal antibody. | human parainfluenza virus 3 (hpiv3) commonly causes respiratory disorders in infants and young children. monoclonal antibodies (mabs) have been produced to several components of hpiv3 and commercially available. however, the utility of these antibodies for several immunological and proteomic assays for understanding the nature of hpiv3 infection remain to be characterized. herein, we report the development and characterization of mabs against hemagglutinin-neuraminidase (hn) of hpiv3. a recombin ... | 2014 | 24860558 |
| concordance between rt-pcr-based detection of respiratory viruses from nasal swabs collected for viral testing and nasopharyngeal swabs collected for bacterial testing. | epidemiologic studies of respiratory infections frequently rely on separate sample collections for the detection of bacteria and viruses. the requirement for two specimens presents cost, logistical, and acceptability challenges. | 2014 | 24875136 |
| etiology of acute bronchiolitis and the relationship with meteorological conditions in hospitalized infants in china. | to investigate the prevalence of common viruses and mycoplasma pneumoniae (mp) in hospitalized infants with acute bronchiolitis and study the relationship between bronchiolitis and meteorological conditions. | 2014 | 24961189 |
| human parainfluenza virus infection in thai children with lower respiratory tract infection from 2010 to 2013. | human parainfluenza virus (hpiv) is a common cause of upper and lower respiratory illness in infants and young children. in order to classify the hpiv isolates circulating in the central part of thailand, 650 samples obtained from the lower respiratory tract of patients from two hospital pediatric wards during 2010 to 2013, were analyzed for the presence and types of hpivs by multiplex semi-nested pcr of hemagglutinin-neuraminidase (hn) gene. the results showed that 4.8% of the samples were posi ... | 2014 | 24974645 |
| parainfluenza virus type 3 ab in allogeneic hematopoietic cell transplant recipients: factors influencing post-transplant ab titers and associated outcomes. | parainfluenza virus type 3 (piv-3) can cause severe respiratory illness among hematopoietic cell transplantation (hct) recipients. factors associated with piv-3-specific ab level, and the association between piv-3 ab levels and clinical outcomes in hct recipients who acquire piv-3 infection, are unknown. we evaluated piv-3-specific hemagglutination inhibition ab levels and clinical outcomes among 172 patients with piv-3 infection following hct. in a multivariable linear regression model, high po ... | 2014 | 24978141 |
| viral etiology and seasonality of influenza-like illness in gabon, march 2010 to june 2011. | surveillance of influenza-like illness (ili) in central africa began only recently, and few data are therefore available on the circulation of influenza virus and other respiratory viruses. in gabon, a central african country, we established a surveillance network in four major towns in order to analyze cases of ili among patients who visited health centers between march 2010 and june 2011, and to determine the viral etiology. | 2014 | 25000832 |
| microorganisms in respiratory tract of patients diagnosed with atypical pneumonia: results of a research based on the use of reverse transcription polymerase chain reaction (rt-pcr) dna microarray method and enzyme-linked immunosorbent assay. | numerous molecular-based tests were applied for the laboratory-based diagnosis of viruses. in this cross-sectional case control study, in addition to bacteria, we aimed to determine respiratory viruses using, for the first time in our country, the reverse transcription pcr dna microarray method, and we also aimed to evaluate its diagnostic performance. | 2014 | 25016709 |
| patterns of detection of respiratory viruses in nasal swabs from calves in ireland: a retrospective study. | a retrospective analysis was conducted to investigate the prevalence and seasonality of bovine viral diarrhoea virus (bvdv), bovine coronavirus (bocv), bovine herpesvirus-1 (bohv-1), bovine respiratory syncytical virus (brsv) and parainfluenza virus-3 (pi3v) in calves (aged three months and below) in ireland. results from real-time pcr testing, including cycle threshold values, conducted on nasal swabs (single or pooled) submitted from 1364 respiratory disease outbreaks between january 1, 2008 a ... | 2014 | 25037889 |
| rational design of human metapneumovirus live attenuated vaccine candidates by inhibiting viral mrna cap methyltransferase. | the paramyxoviruses human respiratory syncytial virus (hrsv), human metapneumovirus (hmpv), and human parainfluenza virus type 3 (hpiv3) are responsible for the majority of pediatric respiratory diseases and inflict significant economic loss, health care costs, and emotional burdens. despite major efforts, there are no vaccines available for these viruses. the conserved region vi (cr vi) of the large (l) polymerase proteins of paramyxoviruses catalyzes methyltransferase (mtase) activities that t ... | 2014 | 25056882 |
| methyltransferase-defective avian metapneumovirus vaccines provide complete protection against challenge with the homologous colorado strain and the heterologous minnesota strain. | avian metapneumovirus (ampv), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. since its discovery in the 1970s, ampv has been recognized as an economically important pathogen in the poultry industry worldwide. the conserved region vi (cr vi) of the large (l) polymerase proteins of paramyxoviruses catalyzes methyltransferase (mtase) activities that typically methylate viral ... | 2014 | 25122790 |
| terminal sialic acid linkages determine different cell infectivities of human parainfluenza virus type 1 and type 3. | human parainfluenza virus type 1 (hpiv1) and type 3 (hpiv3) initiate infection by sialic acid binding. here, we investigated sialic acid linkage specificities for binding and infection of hpiv1 and hpiv3 by using sialic acid linkage-modified cells treated with sialidases or sialyltransferases. the hpiv1 is bound to only α2,3-linked sialic acid residues, whereas hpiv3 is bound to α2,6-linked sialic acid residues in addition to α2,3-linked sialic acid residues in human red blood cells. α2,3 linkag ... | 2014 | 25146600 |
| respiratory virus is a real pathogen in immunocompetent community-acquired pneumonia: comparing to influenza like illness and volunteer controls. | viral pathogens were more commonly reported than previously estimated in community-acquired pneumonia (cap) patients. however, the real role of virus was still controversial. | 2014 | 25178477 |
| a leucine residue in the c terminus of human parainfluenza virus type 3 matrix protein is essential for efficient virus-like particle and virion release. | paramyxovirus particles, like other enveloped virus particles, are formed by budding from membranes of infected cells, and matrix (m) proteins are critical for this process. to identify the m protein important for this process, we have characterized the budding of the human parainfluenza virus type 3 (hpiv3) m protein. our results showed that expression of the hpiv3 m protein alone is sufficient to initiate the release of virus-like particles (vlps). electron microscopy analysis confirmed that v ... | 2014 | 25187547 |
| circulating clinical strains of human parainfluenza virus reveal viral entry requirements for in vivo infection. | human parainfluenza viruses (hpivs) cause widespread respiratory infections, with no vaccines or effective treatments. we show that the molecular determinants for hpiv3 growth in vitro are fundamentally different from those required in vivo and that these differences impact inhibitor susceptibility. hpiv infects its target cells by coordinated action of the hemagglutinin-neuraminidase receptor-binding protein (hn) and the fusion envelope glycoprotein (f), which together comprise the molecular fu ... | 2014 | 25210187 |
| a novel parainfluenza virus type 3 (piv3) identified from goat herds with respiratory diseases in eastern china. | parainfluenza virus type 3 (piv3) is one of the most important viral respiratory pathogens for humans and for many animals, but goat infection has been rarely reported. starting in aug 2013, goats in the jiangsu and anhui provinces of eastern china suffered severe respiratory diseases. in order to identify the causative agent, numerous related pathogens were tested with rt-pcr or pcr. a unique piv3 strain was detected in most of the clinical nasal swabs or serum samples. the virus was isolated o ... | 2014 | 25236986 |
| structure-guided discovery of potent and dual-acting human parainfluenza virus haemagglutinin-neuraminidase inhibitors. | human parainfluenza viruses (hpivs) cause upper and lower respiratory tract disease in children that results in a significant number of hospitalizations and impacts health systems worldwide. to date, neither antiviral drugs nor vaccines are approved for clinical use against parainfluenza virus, which reinforces the urgent need for new therapeutic discovery strategies. here we use a multidisciplinary approach to develop potent inhibitors that target a structural feature within the hpiv type 3 hae ... | 2014 | 25327774 |
| serologic, trace element, and fecal parasite survey of free-ranging, female mule deer (odocoileus hemionus) in eastern washington, usa. | blood and fecal samples collected from 97 free-ranging mule deer (odocoileus hemionus), from four distinct herds during the spring of 2000 or 2001 in eastern washington, us, were tested for exposure to selected pathogens, concentrations of trace elements, and presence of parasites in feces. antibodies were detected to the following: leptospira interrogans serovar bratislava (4%), leptospira interrogans serovar canicola (1%), leptospira interrogans serovar grippotyphosa (13%), bovine viral diarrh ... | 2015 | 25380366 |
| virus profile in children with acute respiratory infections with various severities in beijing, china. | acute respiratory infection (ari) is one of the most common infectious diseases in infants and young children globally. this study aimed to determine the virus profile in children with ari presenting with different severities. | 2014 | 25382323 |
| associations between co-detected respiratory viruses in children with acute respiratory infections. | viruses are the major etiological agents of acute respiratory infections (aris) in young children. although respiratory virus co-detections are common, analysis of combinations of co-detected viruses has never been conducted in japan. nineteen respiratory viruses or subtypes were surveyed using multiplex real-time pcr on 1,044 pediatric (patient age < 6 years) ari specimens collected in osaka city, japan between january 2010 and december 2011. in total, 891 specimens (85.3%) were virus positive ... | 2014 | 25410563 |
| viral respiratory infections among hajj pilgrims in 2013. | middle east respiratory syndrome coronavirus (mers-cov) has emerged in the arabian gulf region, with its epicentre in saudi arabia, the host of the 'hajj' which is the world's the largest mass gathering. transmission of mers-cov at such an event could lead to its rapid worldwide dissemination. therefore, we studied the frequency of viruses causing influenza-like illnesses (ili) among participants in a randomised controlled trial at the hajj 2013. we recruited 1038 pilgrims from saudi arabia, aus ... | 2014 | 25413828 |
| complete genome sequence of the first isolate of genotype c bovine parainfluenza virus type 3 in japan. | bovine parainfluenza virus type 3 (bpiv3) isolates are classified into three genotypes (bpiv3a to -c). here, we report the complete genome sequence of the bpiv3c isolate for the first time in japan. our results indicate that new primer sets will be required to detect all genotypes of bpiv3 strains. | 2014 | 25428970 |
| estimation of nasal shedding and seroprevalence of organisms known to be associated with bovine respiratory disease in australian live export cattle. | the prevalence of organisms known to be associated with bovine respiratory disease (brd) was investigated in cattle prior to export. a quantitative reverse transcription polymerase chain reaction assay was used to detect nucleic acids from the following viruses and bacteria in nasal swab samples: bovine coronavirus (bocv; betacoronavirus 1), bovine herpesvirus 1 (bohv-1), bovine viral diarrhea virus 1 (bvdv-1), bovine respiratory syncytial virus (brsv), bovine parainfluenza virus 3 (bpiv-3), his ... | 2015 | 25525134 |
| infection of the upper respiratory tract of hamsters by the bovine parainfluenza virus type 3 bn-1 strain expressing enhanced green fluorescent protein. | bovine parainfluenza virus type 3 (bpiv3) is an important pathogen associated with bovine respiratory disease complex (brdc). we have generated a recombinant bpiv3 expressing enhanced green fluorescent protein (rbpiv3-egfp) based on the bn-1 strain isolated in japan. after intranasal infection of hamsters with rbpiv3-egfp, egfp fluorescence was detected in the upper respiratory tract including the nasal turbinates, pharynx, larynx, and trachea. in the nasal turbinates, rbpiv3-egfp attained high ... | 2015 | 25543964 |
| the aberrant gene-end transcription signal of the matrix m gene of human parainfluenza virus type 3 downregulates fusion f protein expression and the f-specific antibody response in vivo. | human parainfluenza virus type 3 (hpiv3), a paramyxovirus, is a major viral cause of severe lower respiratory tract disease in infants and children. the gene-end (ge) transcription signal of the hpiv3 matrix (m) protein gene is identical to those of the nucleoprotein and phosphoprotein genes except that it contains an apparent 8-nucleotide insert. this was associated with an increased synthesis of a readthrough transcript of the m gene and the downstream fusion (f) protein gene. we hypothesized ... | 2015 | 25589643 |
| hemagglutinin-neuraminidase gene sequence-based reclassification of human parainfluenza virus 3 variants. | the most comprehensive phylogenetic classification of human parainfluenza virus 3 (hpiv-3) was recently developed [plos one 2012;7:e43893]. this classification included three distinct clusters (a, b and c) with subdivision of cluster c into four subclusters (c1-4). in the present report, the classification of hpiv-3 was refined by inclusion of 27 overlooked beside newly characterized saudi variants. the new phylogram was developed and included the same clusters described before, in which cluster ... | 2015 | 25592955 |
| heparin octasaccharide decoy liposomes inhibit replication of multiple viruses. | heparan sulfate (hs) is a ubiquitous glycosaminoglycan that serves as a cellular attachment site for a number of significant human pathogens, including respiratory syncytial virus (rsv), human parainfluenza virus 3 (hpiv3), and herpes simplex virus (hsv). decoy receptors can target pathogens by binding to the receptor pocket on viral attachment proteins, acting as 'molecular sinks' and preventing the pathogen from binding to susceptible host cells. decoy receptors functionalized with hs could bi ... | 2015 | 25637710 |
| the catalytic mechanism of human parainfluenza virus type 3 haemagglutinin-neuraminidase revealed. | human parainfluenza virus type 3 (hpiv-3) is one of the leading causes for lower respiratory tract disease in children, with neither an approved antiviral drug nor vaccine available to date. understanding the catalytic mechanism of human parainfluenza virus haemagglutinin-neuraminidase (hn) protein is key to the design of specific inhibitors against this virus. herein, we used (1) h nmr spectroscopy, x-ray crystallography, and virological assays to study the catalytic mechanism of the hn enzyme ... | 2015 | 25676091 |
| electron tomography imaging of surface glycoproteins on human parainfluenza virus 3: association of receptor binding and fusion proteins before receptor engagement. | in order to deliver their genetic material to host cells during infection, enveloped viruses use specialized proteins on their surfaces that bind cellular receptors and induce fusion of the viral and host membranes. in paramyxoviruses, a diverse family of single-stranded rna (ssrna) viruses, including several important respiratory pathogens, such as parainfluenza viruses, the attachment and fusion machinery is composed of two separate proteins: a receptor binding protein (hemagglutinin-neuramini ... | 2015 | 25691596 |
| association of bovine respiratory disease or vaccination with serologic response in dairy heifer calves up to three months of age. | to investigate the association of bovine respiratory disease (brd) or vaccination with serologic response in calves. | 2015 | 25710760 |
| human parainfluenza virus type 3 (hpiv3) induces production of ifnγ and rantes in human nasal epithelial cells (hnecs). | human parainfluenza virus type 3 (hpiv3), while infecting lower airway epithelial cells induces pneumonia and bronchiolitis in infants and children, and may lead to asthma exacerbations in children and adults. respiratory viruses invading the airway epithelium activate innate immune response and induce inflammatory cytokine release contributing to the pathophysiology of upper and lower airway disorders. however, the effects of hpiv3 infection on nasal epithelial cells have not been well defined. ... | 2015 | 25722655 |
| cross-protection study of a mannheimia haemolytica serotype 1 vaccine against acute pasteurellosis in lambs induced by a serotype 2 strain. | ovine pneumonia is an economic important disease worldwide for the sheep industry. multiple serotypes (s) of mannheimia haemolytica are involved in the disease and s2 and s1 are the most frequent isolates associated with lung lesions in sheep. vaccines based on some m. haemolytica s2 strains have been shown to have poor immunogenicity. the objective of this study was to determine the cross-protection effect of an s1 strain based vaccine, bovilis mh, in sheep against an experimental challenge wit ... | 2015 | 25843945 |
| amino acid substitutions contributing to α2,6-sialic acid linkage binding specificity of human parainfluenza virus type 3 hemagglutinin-neuraminidase. | human parainfluenza virus type 3 (hpiv3) recognizes both α2,3- and α2,6-linked sialic acids, whereas human parainfluenza virus type 1 (hpiv1) recognizes only α2,3-linked sialic acids. to identify amino acid residues that confer α2,6-linked sialic acid recognition of hpiv3, amino acid residues in or neighboring the sialic acid binding pocket of the hpiv3 hemagglutinin-neuraminidase (hn) glycoprotein were substituted for the corresponding residues of hpiv1 hn. hemadsorption assay with sialyl linka ... | 2015 | 25871520 |
| respiratory viruses and torque teno virus in adults with acute respiratory infections. | to define the molecular epidemiology of respiratory viral infections in adult patients. | 2015 | 25890989 |
| mixed infection of peste des petits ruminants virus (pprv) and other respiratory viruses in dromedary camels in sudan, an abattoir study. | this study was intended to determine the role played by peste des petits ruminants (ppr) in causing respiratory infections in camels and its association with other respiratory viruses. a total of 474 lung specimens showing pneumonia were collected from clinically healthy camels in slaughterhouses at five different areas in sudan. using immunocapture elisa (icelisa), 214 specimens (45.1 %) were found to be positive for ppr antigen. the highest prevalence was found in central sudan (59.9 %) then n ... | 2015 | 25904508 |
| identification of three antigen epitopes on the nucleocapsid protein of the genotype c of bovine parainfluenza virus type 3. | bovine parainfluenza virus type 3 (bpiv3) is an important respiratory tract pathogen for both young and adult cattle. so far, three genotypes a, b and c of bpiv3 have been described on the basis of genetic and phylogenetic analysis. but fine mapping of epitopes of bpiv3 is scant and the antigenic variations among the three genotypes of bpiv3 have not been reported. nucleocapsid protein (np) is the most abundant protein in the virion and highly conserved in bpiv3, which is crucial for the inducti ... | 2015 | 25960335 |
| etiological role of ortho- and paramyxoviruses in acute respiratory tract infections among children aged < 4 years in bulgaria. | influenza viruses (family orthomyxoviridae); respiratory-syncytial virus (rsv), human metapneumovirus (hmpv), and parainfluenza viruses (hpiv) type 1, 2 and 3 (family paramyxoviridae) are among the most common causes of acute respiratory tract infections (arti) in infants and young children. the aim of this study was to determine the contribution of these viruses in cases of arti requiring medical attention among children aged < 4 years during the 2012/13 and 2013/14 winter seasons in bulgaria. | 2015 | 25974986 |
| 3d tissue-like assemblies: a novel approach to investigate virus-cell interactions. | virus-host cell interactions are most commonly analyzed in cells maintained in vitro as two-dimensional tissue cultures. however, these in vitro conditions vary quite drastically from the tissues that are commonly infected in vivo. over the years, a number of systems have been developed that allow the establishment of three-dimensional (3d) tissue structures that have properties similar to their in vivo 3d counterparts. these 3d systems have numerous applications including drug testing, maintena ... | 2015 | 25986169 |
| development and evaluation of two truncated recombinant np antigen-based indirect elisas for detection of bovine parainfluenza virus type 3 antibodies in cattle. | bovine parainfluenza virus type 3 (bpiv3) is one of the most important viral respiratory pathogens in both young and adult cattle. nucleocapsid protein (np) is the most abundant viral protein and the main regulator of virus replication and transcription. in this study, amino acid sequence data of bpiv3 np was used to identify potential linear epitopic regions, which were subsequently used to design truncated recombinant np antigens. the amino-terminal region (aa 9-157, np-n) and the carboxy-term ... | 2015 | 26031225 |
| enhanced neutralizing antibody response induced by respiratory syncytial virus prefusion f protein expressed by a vaccine candidate. | respiratory syncytial virus (rsv) and human parainfluenza virus type 3 (hpiv3) are the first and second leading viral agents of severe respiratory tract disease in infants and young children worldwide. vaccines are not available, and an rsv vaccine is particularly needed. a live attenuated chimeric recombinant bovine/human piv3 (rb/hpiv3) vector expressing the rsv fusion (f) glycoprotein from an added gene has been under development as a bivalent vaccine against rsv and hpiv3. previous clinical ... | 2015 | 26157122 |
| aerosolized ebola vaccine protects primates and elicits lung-resident t cell responses. | direct delivery of aerosolized vaccines to the respiratory mucosa elicits both systemic and mucosal responses. this vaccine strategy has not been tested for ebola virus (ebov) or other hemorrhagic fever viruses. here, we examined the immunogenicity and protective efficacy of an aerosolized human parainfluenza virus type 3-vectored vaccine that expresses the glycoprotein (gp) of ebov (hpiv3/ebogp) delivered to the respiratory tract. rhesus macaques were vaccinated with aerosolized hpiv3/ebogp, li ... | 2015 | 26168222 |
| novel atlantic bottlenose dolphin parainfluenza virus ttpiv-1 clusters with bovine piv-3 genotype b strains. | parainfluenza virus 3 (piv-3) is a common viral infection not only in humans, but also in many other species. serological evidence suggests that nearly 100 % of children in the united states have been infected with piv-3 by 5 years of age. similarly, in cattle, piv-3 is commonly associated with bovine respiratory disease complex. a novel dolphin piv-3 (ttpiv-1) was described by nollens et al. in 2008 from a dolphin that was diagnosed with an unknown respiratory illness. at that time, ttpiv-1 was ... | 2015 | 26174699 |
| epidemiology and clinical outcome of lower respiratory tract infections by respiratory syncytial virus or parainfluenza virus type 3 in adults receiving treatment for either acute leukemia or severe aplastic anemia: a retrospective single center study. | 2015 | 26204825 | |
| prevalence of human parainfluenza virus in patients with acute respiratory tract infections in beijing, 2011-2014. | human parainfluenza viruses (hpivs) are one of the most frequent pathogens that circulate worldwide([1]) . four types of hpivs were identified, including hpiv-1, hpiv-2, hpiv-3 and hpiv-4. hpivs can lead to acute respiratory infection in young([2]) , the immunocompromised([3]) and the elderly patients([4]) . the epidemiological character of parainfluenza virus in beijing has not been previously reported. in a consecutive 4-year surveillance of acute respiratory infections (aris) between january ... | 2015 | 26230490 |
| mutations in the di-dii linker of human parainfluenza virus type 3 fusion protein result in diminished fusion activity. | human parainfluenza virus type 3 (hpiv3) can cause severe respiratory tract diseases in infants and young children, but no licensed vaccines or antiviral agents are currently available for treatment. fusing the viral and target cell membranes is a prerequisite for its entry into host cells and is directly mediated by the fusion (f) protein. although several domains of f are known to have important effects on regulating the membrane fusion activity, the roles of the di-dii linker (residues 369-37 ... | 2015 | 26305905 |
| catalytic mechanism and novel receptor binding sites of human parainfluenza virus type 3 hemagglutinin-neuraminidase (hpiv3 hn). | the human parainfluenza virus type 3 (hpiv3) hemagglutinin-neuraminidase (hn) has opposing functions of binding sialic acid receptors and cleaving them, facilitating virus release. the crystal structure of hpiv3 hn complexed with the substrate analogue difluorosialic acid (dfsa) revealed that catalysis by hn involves the formation of a covalently linked sialosyl-enzyme intermediate which was trapped along with a transition-state analogue resembling an oxocarbenium ion. this mechanism of enzyme c ... | 2015 | 26364554 |
| regulation of viral rna synthesis by the v protein of parainfluenza virus 5. | paramyxoviruses include many important animal and human pathogens. the genome of parainfluenza virus 5 (piv5), a prototypical paramyxovirus, encodes a v protein that inhibits viral rna synthesis. in this work, the mechanism of inhibition was investigated. using mutational analysis and a minigenome system, we identified regions in the n and c termini of the v protein that inhibit viral rna synthesis: one at the very n terminus of v and the second at the c terminus of v. furthermore, we determined ... | 2015 | 26378167 |
| serological detection of infection dynamics for respiratory viruses among dairy calves. | the aim of this study is to reveal infection dynamics of bovine respiratory syncytial virus (brsv), bovine parainfluenza virus type 3 (pi-3), bovine herpesvirus 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus type 3 (bav-3) and bovine coronavirus (bcov), which are important viral pathogens of respiratory disease complex in ruminants. through such an analysis, the regression period of maternally derived antibodies and optimum vaccination time in calves can be recommended. a total ... | 2015 | 26380946 |
| virucidal action of sore throat lozenges against respiratory viruses parainfluenza type 3 and cytomegalovirus. | most respiratory tract infections are self-limiting and caused by viruses, and do not warrant antibiotic treatment. despite this, patients with respiratory tract infections often receive antibiotics, fuelling the rise of antibiotic resistance. therefore, there is a need to encourage patients to try alternative non-antibiotic therapies, which ideally treat the symptoms and the cause. lozenges containing amylmetacresol and 2,4-dichlorobenzyl alcohol (amc/dcba lozenges) as well as lozenges containi ... | 2015 | 26408353 |
| safety of inoculation of bovine parainfluenza virus 3 as potential vaccine vector in pigs. | bovine parainfluenza virus 3 (bpiv3) is one of the most important respiratory pathogens in cattle. one bpiv3, named nm09, was isolated from cattle suffering from severe respiratory diseases in 2009. bpiv3 is a potential recombinant vaccine vector. to investigate whether nm09 can infect pigs and determine bpiv3 defense in these animals, bpiv3 antibody-free pigs were inoculated intramuscularly with the bpiv3 nm09 strain in a continuous passage. clinical signs were observed each day after inoculati ... | 2015 | 26436127 |
| parainfluenza virus types 1, 2, and 3 in pediatric patients with acute respiratory infections in beijing during 2004 to 2012. | although human parainfluenza virus (hpiv) has been determined as an important viral cause of acute respiratory infections (aris) in infants and young children, data on long-term investigation are still lacking to disclose the infection pattern of hpiv in china. | 2015 | 26481737 |
| the clinical usefulness of lymphocyte:monocyte ratios in differentiating influenza from viral non-influenza-like illnesses in hospitalized adults during the 2015 influenza a (h3n2) epidemic: the uniqueness of hpiv-3 mimicking influenza a. | during influenza epidemics, influenza-like illnesses (ilis) viruses cocirculate with influenza strains. if positive, rapid influenza diagnostic tests (ridts) identify influenza a/b, but false-negative ridts require retesting by viral polymerase chain reaction (pcr). patient volume limits testing during influenza epidemics, and non-specific laboratory findings have been used for presumptive diagnosis pending definitive viral testing. in adults, the most useful laboratory abnormalities in influenz ... | 2016 | 26563893 |
| recent advances in the development of vaccines for ebola virus disease. | ebola virus is one of the most dangerous microorganisms in the world causing hemorrhagic fevers in humans and non-human primates. ebola virus (ebov) is a zoonotic infection, which emerges and re-emerges in human populations. the 2014 outbreak was caused by the zaire strain, which has a kill rate of up to 90%, though 40% was recorded in the current outbreak. the 2014 outbreak is larger than all 20 outbreaks that have occurred since 1976, when the virus was first discovered. it is the first time t ... | 2016 | 26596227 |
| analysis on the complete genome of a novel caprine parainfluenza virus 3. | parainfluenza virus type 3 (piv3) is one of the most important viral respiratory pathogens for humans and for many animals. one unique caprine piv3 (cpiv3) strain named js2013 was isolated in chinese goat flocks with respiratory diseases in 2013. now, the complete genome sequence of the strain js2013 had been determined. a total of 15 overlapping dna clones, covering the entire genome of the virus, were obtained by primer walking rt-pcr. the sequences of the 3' and 5' termini of the viral genome ... | 2016 | 26631811 |
| interaction of human parainfluenza virus type 3 nucleoprotein with matrix protein mediates internal viral protein assembly. | human parainfluenza virus type 3 (hpiv3) belongs to the paramyxoviridae family. its three internal viral proteins, the nucleoprotein (n), the phosphoprotein (p), and the polymerase (l), form the ribonucleoprotein (rnp) complex, which encapsidates the viral genome and associates with the matrix protein (m) for virion assembly. we previously showed that the m protein expressed alone is sufficient to assemble and release virus-like particles (vlps) and a mutant with the l305a point mutation in the ... | 2015 | 26656716 |
| isolation & molecular characterization of human parainfluenza virus in chennai, india. | human parainfluenza virus (hpiv) accounts for a significant proportion of lower respiratory tract infections in children as well as adults. this study was done to detect the presence of different subtypes of hpiv from patients having influenza like illness (ili). | 2015 | 26658594 |
| mutations in the leucine zipper-like motif of the human parainfluenza virus 3 fusion protein impair fusion activity. | to investigate the effect of the leucine zipper-like motif between hra and hrb of the human parainfluenza virus 3 fusion protein on fusion activity. | 2015 | 26694747 |
| [association between parainfluenza virus infection and climatic factors in children]. | to study the association between acute respiratory human parainfluenza virus (hpiv) infection and climatic factors in children. | 2015 | 26695668 |
| parainfluenza virus 3 blocks antiviral mediators downstream of the interferon lambda receptor by modulating stat1 phosphorylation. | parainfluenza viruses are known to inhibit type i interferon (ifn) production; however, there is a lack of information regarding the type iii ifn response during infection. type iii ifns signal through a unique heterodimeric receptor, ifn-λr1/interleukin-10r2 (il-10r2), which is primarily expressed by epithelial cells. parainfluenza virus 3 (piv-3) infection is highly restricted to the airway epithelium. we therefore sought to examine type iii ifn signaling pathways during piv-3 infection of epi ... | 2015 | 26719274 |
| real-time detection of a virus using detection dogs. | viral infections are ubiquitous in humans, animals, and plants. real-time methods to identify viral infections are limited and do not exist for use in harsh or resource-constrained environments. previous research identified that tissues produce unique volatile organic compounds (voc) and demonstrated that voc concentrations change during pathologic states, including infection, neoplasia, or metabolic disease. patterns of voc expression may be pathogen specific and may be associated with an odor ... | 2015 | 26779494 |
| phylogenetic and molecular analyses of human parainfluenza type 3 virus in buenos aires, argentina, between 2009 and 2013: the emergence of new genetic lineages. | despite that human parainfluenza type 3 viruses (hpiv3) are one of the leading causes of acute lower respiratory tract infections in children under five, there is no licensed vaccine and there is limited current information on the molecular characteristics of regional and global circulating strains. the aim of this study was to describe the molecular characterization of hpiv3 circulating in buenos aires. we performed a genetic and phylogenetic analysis of the hn glycoprotein gene. between 2009 a ... | 2016 | 26780643 |
| erratum for gui et al., electron tomography imaging of surface glycoproteins on human parainfluenza virus 3: association of receptor binding and fusion proteins before receptor engagement. | 2016 | 26787830 | |
| incidence and clinical course of respiratory viral coinfections in children aged 0-59 months. | clinical data available on coinfections are contradictory concerning both the number of viruses involved and the severity of the condition. a total of 114 patients aged 0-59 months with symptoms of respiratory tract infection were enrolled into the study. nasal and pharyngeal swabs were tested using the pcr method for the following 12 viruses: influenza a, influenza b, respiratory syncytial virus a (rsv a), respiratory syncytial virus b (rsv b), adenovirus, metapneumovirus, coronavirus 229e/nl63 ... | 2016 | 26801151 |
| evaluation of epidemiological and clinical features of influenza and other respiratory viruses. | in our study, we aimed to clinically and epidemiologically evaluate respiratory tract infections the viral agents of which were detected by molecular methods and to compare influenza and other respiratory tract viruses in this context. | 2015 | 26884691 |
| das181 treatment of severe parainfluenza virus 3 pneumonia in allogeneic hematopoietic stem cell transplant recipients requiring mechanical ventilation. | parainfluenza virus (piv) may cause life-threatening pneumonia in allogeneic hematopoietic stem cell transplant (hsct) recipients. currently, there are no proven effective therapies. we report the use of inhaled das181, a novel sialidase fusion protein, for treatment of piv type 3 pneumonia in two allogeneic hematopoietic sct recipients with respiratory failure. | 2016 | 26941799 |
| prevalence of respiratory viruses among children hospitalized from respiratory infections in shenzhen, china. | the prevalence of local dominant viral etiologies is important for clinical management and prevention of common viral respiratory tract infections. unfortunately, there is limited large-scale data about common viral respiratory infection in south china. to survey dominant viral etiology and seasonality of acute respiratory infections in hospitalized children, a 4-year consecutive study was conducted in shenzhen, china. | 2016 | 26952107 |
| [analysis of pathogenic features and infection status of human parainfluenza virus type 3 among children in hangzhou]. | to determine the level of genetic variation of human parainfluenza virus type 3 (hpiv-3), and to describe infection and co-infection characteristics of hpiv-3 in children. | 2016 | 26957245 |
| viral protein requirements for assembly and release of human parainfluenza virus type 3 virus-like particles. | to understand the roles of human parainfluenza virus 3 (hpiv3) proteins in assembly and release, viral proteins were expressed individually and in combination in 293t cells. expression of the matrix (m) protein triggered release of enveloped, matrix-containing virus-like particles (vlps) from cells. when m was co-expressed with the nucleocapsid (n), fusion (f) or haemagglutinin-neuraminidase (hn) proteins, vlps that contained m+n, m+f and m+hn, respectively, were generated, suggesting that m can ... | 2016 | 26960133 |
| associations between exposure to viruses and bovine respiratory disease in australian feedlot cattle. | bovine respiratory disease (brd) is the most important cause of clinical disease and death in feedlot cattle. respiratory viral infections are key components in predisposing cattle to the development of this disease. to quantify the contribution of four viruses commonly associated with brd, a case-control study was conducted nested within the national bovine respiratory disease initiative project population in australian feedlot cattle. effects of exposure to bovine viral diarrhoea virus 1 (bvdv ... | 2016 | 26972273 |
| features of circulating parainfluenza virus required for growth in human airway. | respiratory paramyxoviruses, including the highly prevalent human parainfluenza viruses, cause the majority of childhood croup, bronchiolitis, and pneumonia, yet there are currently no vaccines or effective treatments. paramyxovirus research has relied on the study of laboratory-adapted strains of virus in immortalized cultured cell lines. we show that findings made in such systems about the receptor interaction and viral fusion requirements for entry and fitness-mediated by the receptor binding ... | 2016 | 26980833 |
| adult human metapneumonovirus (hmpv) pneumonia mimicking legionnaire's disease. | in adults hospitalized with viral pneumonias the main differential diagnostic consideration is influenza pneumonia. the respiratory viruses causing viral influenza like illnesses (ilis), e.g., rsv may closely resemble influenza. rarely, extrapulmonary findings of some ilis may resemble legionnaire's disease (ld), e.g., adenovirus, human parainfluenza virus (hpiv-3). we present a most unusual case of human metapneumonovirus pneumonia (hmpv) with some characteristic extrapulmonary findings charact ... | 2016 | 26988110 |
| human sentinel surveillance of influenza and other respiratory viral pathogens in border areas of western cambodia. | little is known about circulation of influenza and other respiratory viruses in remote populations along the thai-cambodia border in western cambodia. we screened 586 outpatients (median age 5, range 1-77) presenting with influenza-like-illness (ili) at 4 sentinel sites in western cambodia between may 2010 and december 2012. real-time reverse transcriptase (rrt) pcr for influenza was performed on combined nasal and throat specimens followed by viral culture, antigenic analysis, antiviral suscept ... | 2016 | 27028323 |
| clinical evaluation of a single-tube multiple rt-pcr assay for the detection of 13 common virus types/subtypes associated with acute respiratory infection. | respiratory viruses are among the most important causes of human morbidity and mortality worldwide, especially for infants and young children. in the past years, a few commercial multiplex rt-pcr assays have been used to detect respiratory viruses in spite of the high cost. in the present study, an improved single-tube multiplex reverse transcription pcr assay for simultaneous detection of 13 respiratory viruses was evaluated and compared with a previously reported two-tube assay as the referenc ... | 2016 | 27043208 |
| a dual drug regimen synergistically blocks human parainfluenza virus infection. | human parainfluenza type-3 virus (hpiv-3) is one of the principal aetiological agents of acute respiratory illness in infants worldwide and also shows high disease severity in the elderly and immunocompromised, but neither therapies nor vaccines are available to treat or prevent infection, respectively. using a multidisciplinary approach we report herein that the approved drug suramin acts as a non-competitive in vitro inhibitor of the hpiv-3 haemagglutinin-neuraminidase (hn). furthermore, the d ... | 2016 | 27053240 |
| [phylogenetic variability of human metapneumovirus strains circulating in turkey during two consecutive epidemic seasons]. | human metapneumovirus (hmpv), classified in paramyxoviridae family, phylogenetically consists of two major groups namely a and b, with genetic lineages of a1, a2 (comprises of sublineages a2a and a2b) and b1, b2. although detailed evaluation on phylogenetic analysis of hmpv has been described in other countries, there are no data from turkey on this subject. the aim of this study was to demonstrate for the first time, the phylogenetic diversity of hmpv strains circulating in turkey during two co ... | 2016 | 27058330 |
| a reverse genetics approach for the design of methyltransferase-defective live attenuated avian metapneumovirus vaccines. | avian metapneumovirus (ampv), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. ampv belongs to the family paramyxoviridae which includes many important human pathogens such as human respiratory syncytial virus (rsv), human metapneumovirus (hmpv), and human parainfluenza virus type 3 (piv3). the family also includes highly lethal emerging pathogens such as nipah virus and hen ... | 2016 | 27076293 |
| clinical evaluation of the new high-throughput luminex nxtag respiratory pathogen panel assay for multiplex respiratory pathogen detection. | a broad range of viral and bacterial pathogens can cause acute respiratory tract infection. for rapid detection of a broad respiratory pathogen spectrum, multiplex real-time pcr is ideal. this study evaluated the performance of the new luminex nxtag respiratory pathogen panel (nxtag-rpp) in comparison with the biofire filmarray respiratory panel (fa-rp) or singleplex real-time pcr as reference. a total of 284 clinical respiratory specimens and 3 influenza a/h7n9 viral culture samples were tested ... | 2016 | 27122380 |
| efficacy of intranasal vaccination with a multivalent vaccine containing temperature-sensitive modified-live bovine herpesvirus type 1 for protection of seronegative and seropositive calves against respiratory disease. | objective to evaluate efficacy and duration of immunity of the bovine herpesvirus type 1 (bhv-1) fraction of a trivalent vaccine also containing parainfluenza virus-3 and bovine respiratory syncytial virus fractions administered intranasally (in) for protection of calves against infectious bovine rhinotracheitis (ibr). design controlled challenge study. animals 120 dairy calves (3 to 8 days old) seronegative for antibody against bhv-1 (experiments 1 and 2) or seropositive for maternally derived ... | 2016 | 27172345 |
| human parainfluenza virus types 1-4 in hospitalized children with acute lower respiratory infections in china. | human parainfluenza viruses (hpivs) are an important cause of acute lower respiratory tract infections (alrtis). hpiv-4, a newly identified virus, has been associated with severe alrtis recently. a total of 771 nasopharyngeal aspirate samples were collected from hospitalized children between march 2010 and february 2011. hpivs were detected by nest-pcr, and other known respiratory viruses were detected by rt-pcr and pcr. all amplification products were sequenced. hpivs were detected in 151 (19.5 ... | 2016 | 27197630 |
| cyclopiazonic acid, an inhibitor of calcium-dependent atpases with antiviral activity against human respiratory syncytial virus. | human respiratory syncytial virus (rsv) is a common cause of lower respiratory tract infections in infants and young children worldwide, yet no vaccine or effective antiviral treatment is available. to search for new anti-rsv agents, we developed a cell-based assay that measures inhibition of rsv-induced cytopathic effect (cpe) and identified cyclopiazonic acid (cpa), an intracellular calcium atpase inhibitor as a rsv inhibitor (ec50 values 4.13 μm) by screening of natural product library. cpa i ... | 2016 | 27210812 |
| are atypical lymphocytes present with viral influenza-like illnesses (ilis) in hospitalized adults? | the purpose of this investigation was to determine if atypical lymphocytes were of diagnostic value in viral influenza-like illnesses (ilis) in hospitalized adults during the influenza season. are atypical lymphocytes present with viral ilis in hospitalized adults? during the influenza season, hospitals are inundated with influenza and viral ilis, e.g., human parainfluenza virus-3 (hpiv-3). without specific testing, clinically, it is difficult to differentiate influenza from ilis, and surrogate ... | 2016 | 27250631 |
| pathogenicity and horizontal transmission studies of caprine parainfluenza virus type 3 js2013 strain in goats. | parainfluenza virus type 3 (piv3) is one of the most important viral respiratory pathogens for humans and for many animals, but goat infection has been rarely reported. in 2014, one novel piv3 strain was first isolated from goats suffered respiratory diseases in jiangsu and anhui provinces of eastern china and named as caprine piv3 (cpiv3) js2013. in order to systematically evaluate the pathogenicity and horizontal transmission ability of this new virus, experimental infection of goats with the ... | 2016 | 27374058 |
| sendai virus induces persistent olfactory dysfunction in a murine model of pvod via effects on apoptosis, cell proliferation, and response to odorants. | viral infection is a common cause of olfactory dysfunction. the complexities of studying post-viral olfactory loss in humans have impaired further progress in understanding the underlying mechanism. recently, evidence from clinical studies has implicated parainfluenza virus 3 as a causal agent. an animal model of post viral olfactory disorders (pvod) would allow better understanding of disease pathogenesis and represent a major advance in the field. | 2016 | 27428110 |
| rapid detection of novel caprine parainfluenza virus type 3 (cpiv3) using a taqman-based rt-qpcr. | parainfluenza virus type 3 (piv3) is one of the most important respiratory pathogens for humans and many animals. a novel caprine piv3 (cpiv3) was recently identified and isolated from chinese goat flocks with respiratory disease. in order to develop rapid and sensitive methods for cpiv3 detection in infected goats, a taqman rt-qpcr was established in this study based on the primers and probe designed to amplify a 150 nucleotide-long region located within the m gene of the virus. the method was ... | 2016 | 27448824 |
| epidemiology and etiology of influenza-like-illness in households in vietnam; it's not all about the kids! | household studies provide opportunities to understand influenza-like-illness (ili) transmission, but data from (sub)tropical developing countries are scarce. | 2016 | 27479176 |
| universal mask usage for reduction of respiratory viral infections after stem cell transplant: a prospective trial. | respiratory viral infections (rvis) are frequent complications of hematopoietic stem cell transplant (hsct). surgical masks are a simple and inexpensive intervention that may reduce nosocomial spread. | 2016 | 27481873 |