Publications

potent nematicidal activity of maleimide derivatives on meloidogyne incognita.	different maleimide derivatives were synthesized and assayed for their in vitro activity on the soil inhabiting, plant-parasitic nematode meloidogyne incognita, also known as root-knot nematode. the compounds maleimide, n-ethylmaleimide, n-isopropylmaleimide, and n-isobutylmaleimide showed the strongest nematicidal activity on the second stage juveniles of the root-knot nematode with ec50/72h values of 2.6 ± 1.3, 5.1 ± 3.4, 16.2 ± 5.4, and 19.0 ± 9.0 mg/l, respectively. we also determined the ne ...	2016	27249054
biological control of meloidogyne incognita by aspergillus niger f22 producing oxalic acid.	restricted usage of chemical nematicides has led to development of environmentally safe alternatives. a culture filtrate of aspergillus niger f22 was highly active against meloidogyne incognita with marked mortality of second-stage juveniles (j2s) and inhibition of egg hatching. the nematicidal component was identified as oxalic acid by organic acid analysis and gas chromatography-mass spectroscopy (gc-ms). exposure to 2 mmol/l oxalic acid resulted in 100% juvenile mortality at 1 day after treat ...	2016	27258452
influence of root-knot nematode infestation on antioxidant enzymes, chlorophyll content and growth in pogostemon cablin (blanco) benth.	plants adapt themselves to overcome adverse environmental conditions, and this involves a plethora of concurrent cellular activities. physiological experiments or metabolic profiling can quantify this response. among several diseases of pogostemon cablin (blanco) benth. (patchouli), root-knot nematode infection caused by meloidogyne incognita (kofoid and white) chitwood causes severe damage to the plant and hence, the oil production. in the present study, we identified m. incognita morphological ...	2016	27295922
synthesis, nematocidal activity and docking study of novel chiral 1-(3-chloropyridin-2-yl)-3-(trifluoromethyl)-1h-pyrazole-4-carboxamide derivatives.	a series of novel chiral fluorinated pyrazole carboxamides derivatives were designed and synthesized. all these title compounds were confirmed by nmr and ms. the primarily nematocidal activity results indicated that some of them exhibited good control efficacy against the tomato root-knot nematode disease caused by meloidogyne incognita. the docking results indicated that compound 5n interact with amino acid residue tyr 121, trp 279 of ache via hydrogen bond.	2016	27301365
redirection of auxin flow in arabidopsis thaliana roots after infection by root-knot nematodes.	plant-parasitic root-knot nematodes induce the formation of giant cells within the plant root, and it has been recognized that auxin accumulates in these feeding sites. here, we studied the role of the auxin transport system governed by aux1/lax3 influx proteins and different pin efflux proteins during feeding site development in arabidopsis thaliana roots. data generated via promoter-reporter line and protein localization analyses evoke a model in which auxin is being imported at the basipetal ...	2016	27312670
coupling of mic-3 overexpression with the chromosomes 11 and 14 root-knot nematode (rkn) (meloidogyne incognita) resistance qtls provides insights into the regulation of the rkn resistance response in upland cotton (gossypium hirsutum).	genetic analysis of mic-3 transgene with rkn resistance qtls provides insight into the resistance regulatory mechanism and provides a framework for testing additional hypotheses. resistance to root-knot nematode (rkn) (meloidogyne incognita) in upland cotton (gossypium hirsutum) is mediated by two major quantitative trait loci (qtl) located on chromosomes 11 and 14. the mic-3 (meloidogyne induced cotton3) protein accumulates specifically within the immature galls of rkn-resistant plants that pos ...	2016	27314265
untargeted metabolomics of tomato plants after root-knot nematode infestation.	after 2 months from the infestation of tomato plants with the root-knot nematode (rkn) meloidogyne incognita, we performed a gas chromatography-mass spectrometry untargeted fingerprint analysis for the identification of characteristic metabolites and biomarkers. principal component analysis, and orthogonal projections to latent structures discriminant analysis suggested dramatic local changes of the plant metabolome. in the case of tomato leaves, β-alanine, phenylalanine, and melibiose were indu ...	2016	27389052
reproduction of meloidogyne incognita race 3 on flue-cured tobacco homozygous for rk1 and/or rk2 resistance genes.	most commercial tobacco cultivars possess the rk1 resistance gene to races 1 and 3 of meloidogyne incognita and race 1 of meloidogyne arenaria, which has caused a shift in population prevalence in virginia tobacco fields toward other species and races. a number of cultivars now also possess the rk2 gene for root-knot resistance. experiments were conducted in 2013 to 2014 to examine whether possessing both rk1 and rk2 increases resistance to a variant of m. incognita race 3 compared to either gen ...	2016	27418700
synthesis and biological evaluation of 3,5-dimethoxystilbene analogs.	in our continuing effort to discover natural product-based pest management agents, derivatives of 3,5-dimethoxystilbene were synthesized yielding 27 new and six known compounds. compounds 11 and 12 showed strong aedes aegypti larvicidal activity (lc50 45.31 and 49.93 μm, respectively). furthermore, 11 and 12 exhibited high effectiveness against larvae of pesticide-susceptible and pyrethroid-resistant strains of ae. aegypti; activity against the adult mosquitoes was low. compounds 6f, 6g, and 6i ...	2016	27450661
the fungal endophyte chaetomium globosum negatively affects both above- and belowground herbivores in cotton.	mutualistic plant-endophyte symbioses can benefit plants by increasing host fitness through reductions in herbivory. the fungus, chaetomium globosum strain tamu 520, was previously isolated as an endophyte from cotton (gossypium hirsutum) and can be re-inoculated to systemically colonize cotton plants via seed treatment. we evaluated the potential impacts of the endophyte in cotton on plant parasitic nematodes belowground, along with piercing-sucking and chewing insects aboveground. endophytic c ...	2016	27451418
interactions between the oomycete pythium arrhenomanes and the rice root-knot nematode meloidogyne graminicola in aerobic asian rice varieties.	aerobic rice fields are frequently infested by pathogenic oomycetes (pythium spp.) and the rice root-knot nematode meloidogyne graminicola. here, the interaction between pythium arrhenomanes and meloidogyne graminicola was studied in rice roots of two aerobic rice varieties. in different experimental set-ups and infection regimes, plant growth, rice yield, pythium colonization, as well as establishment, development and reproduction of m. graminicola were studied.	2016	27473143
expression of a cystatin transgene in eggplant provides resistance to root-knot nematode, meloidogyne incognita.	root-knot nematodes (rkn) cause substantial yield decline in eggplant and sustainable management options to minimize crop damage due to nematodes are still limited. a number of genetic engineering strategies have been developed to disrupt the successful plant-nematode interactions. among them, delivery of proteinase inhibitors from the plant to perturb nematode development and reproduction is arguably the most effective strategy. in the present study, transgenic eggplant expressing a modified ri ...	2016	27516765
bioactivity of ruta graveolens and satureja montana essential oils on solanum tuberosum hairy roots and solanum tuberosum hairy roots with meloidogyne chitwoodi co-cultures.	as a nematotoxics screening biotechnological system, solanum tuberosum hairy roots (sthr) and s. tuberosum hairy roots with meloidogyne chitwoodi co-cultures (sthr/crkn) were evaluated, with and without the addition of the essential oils (eos) of satureja montana and ruta graveolens. eos nematotoxic and phytotoxic effects were followed weekly by evaluating nematode population density in the co-cultures as well as growth and volatile profiles of both in vitro cultures types. growth, measured by t ...	2016	27653470
direct nematicidal effects of methyl jasmonate and acibenzolar-s-methyl against meloidogyne incognita.	the aim of this study was to examine the nematicidal properties of two defence inducers against the root-knot nematode meloidogyne incognita. a direct-contact bioassay was applied to evaluate the nematicidal effects of acibenzolar-s-methyl (asm) and methyl jasmonate (meja) on second-stage juveniles (j2). nematodes were incubated in different concentrations of these compounds, and the numbers of immobile nematodes were counted after 24 and 48 h post incubation. tap water was then added to verify ...	2017	27658537
cazyme content of pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism.	pochonia chlamydosporia is a soil fungus with a multitrophic lifestyle combining endophytic and saprophytic behaviors, in addition to a nematophagous activity directed against eggs of root-knot and other plant parasitic nematodes. the carbohydrate-active enzymes encoded by the genome of p. chlamydosporia suggest that the endophytic and saprophytic lifestyles make use of a plant cell wall polysaccharide degradation machinery that can target cellulose, xylan and, to a lesser extent, pectin. this e ...	2016	27668983
the infection of cucumber (cucumis sativus l.) roots by meloidogyne incognita alters the expression of actin-depolymerizing factor (adf) genes, particularly in association with giant cell formation.	cucumber (cucumis sativus l.) is threatened by substantial yield losses due to the south root-knot nematode (meloidogyne incognita). however, understanding of the molecular mechanisms underlying the process of nematode infection is still limited. in this study, we found that m. incognita infection affected the structure of cells in cucumber roots and treatment of the cytoskeleton inhibitor (cytochalasin d) reduced root-knot nematode (rkn) parasitism. it is known that actin-depolymerizing factor ...	2016	27695469
evaluation of steam and soil solarization for meloidogyne arenaria control in florida floriculture crops.	steam and soil solarization were investigated for control of the root-knot nematode meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in florida. the objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (mebr). trials were conducted in a field with naturally occurring populations of m. arenaria. treatments were solarization alone, steam treatment after sol ...	2016	27765992
stage-wise identification and analysis of mirna from root-knot nematode meloidogyne incognita.	in this study, we investigated global changes in mirnas of meloidogyne incognita throughout its life cycle. small rna sequencing resulted in approximately 62, 38, 38, 35, and 39 mb reads in the egg, j2, j3, j4, and female stages, respectively. overall, we identified 2724 known and 383 novel mirnas (read count > 10) from all stages, of which 169 known and 13 novel mirna were common to all the five stages. among the stage-specific mirnas, mir-286 was highly expressed in eggs, mir-2401 in j2, mir-8 ...	2016	27775666
shifting from priming of salicylic acid- to jasmonic acid-regulated defences by trichoderma protects tomato against the root knot nematode meloidogyne incognita.	beneficial root endophytes such as trichoderma spp. can reduce infections by parasitic nematodes through triggering host defences. little is currently known about the complex hormone signalling underlying the induction of resistance. in this study, we investigated whether trichoderma modulates the hormone signalling network in the host to induce resistance to nematodes. we investigated the role and the timing of the jasmonic acid (ja)- and salicylic acid (sa)-regulated defensive pathways in tric ...	2017	27801946
methyl transfer in glucosinolate biosynthesis mediated by indole glucosinolate o-methyltransferase 5.	indole glucosinolates (igs) are plant secondary metabolites that are derived from the amino acid tryptophan. the product of arabidopsis (arabidopsis thaliana) ig core biosynthesis, indol-3-ylmethyl glucosinolate (i3m), can be modified by hydroxylation and subsequent methoxylation of the indole ring in position 1 (1-ig modification) or 4 (4-ig modification). products of the 4-ig modification pathway mediate plant-enemy interactions and are particularly important for arabidopsis innate immunity. w ...	2016	27810943
response of malpighia emarginata active germplasm bank accessions to meloidogyne enterolobii parasitism.	malpighia emarginata is cultivated in almost all brazil and is considered an important agricultural crop. the root-knot nematode meloidogyne enterolobii has been described as a major threat to this crop, causing great production losses. due to the scarcity of information about the severity of this parasite in m. emarginata plants in brazil, this study investigated m. enterolobii resistance of ten m. emarginata genotypes from the active germplasm bank of universidade federal rural de pernambuco. ...	2016	27819718
opda has key role in regulating plant susceptibility to the root-knot nematode meloidogyne hapla in arabidopsis.	jasmonic acid (ja) is a plant hormone that plays important roles in regulating plant defenses against necrotrophic pathogens and herbivorous insects, but the role of ja in mediating the plant responses to root-knot nematodes has been unclear. here we show that an application of either methyl jasmonate (meja) or the ja-mimic coronatine (cor) on arabidopsis significantly reduced the number of galls caused by the root-knot nematode meloidogyne hapla. interestingly, the meja-induced resistance was i ...	2016	27822219
resistance-breaking population of meloidogyne incognita utilizes plant peroxidase to scavenge reactive oxygen species, thereby promoting parasitism on tomato carrying mi-1 gene.	resistance conferred by the mi-1 gene from solanum peruvianum is effective and widely used for controlling root-knot nematodes (rkns, meloidogyne spp.). however, breakdown of resistance by rkns seriously threatens the durable application of the resistance resource. here, a resistance-breaking population of m. incognita was selected from an avirulent population by continuously inoculating on mi-1-carrying tomato. histological observations showed the resistance-breaking population would not induce ...	2017	27836538
genotyping of single spore isolates of a pasteuria penetrans population occurring in florida using snp-based markers.	to generate single spore lines of a population of bacterial parasite of root-knot nematode (rkn), pasteuria penetrans, isolated from florida and examine genotypic variation and virulence characteristics exist within the population.	2017	27862724
mutations in acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode meloidogyne incognita.	the root-knot nematode meloidogyne incognita causes severe damage to continuously cropping vegetables. the control of this nematode relies heavily on organophosphate nematicides in china. here, we described resistance to the organophosphate nematicide fosthiazate in a greenhouse-collected resistant population (rp) and a laboratory susceptible population (sp) of m. incognita. fosthiazate was 2.74-fold less toxic to nematodes from rp than that from sp. quantitative real-time pcr revealed that the ...	2016	27897265
transcriptome analysis of resistant and susceptible tobacco (nicotiana tabacum) in response to root-knot nematode meloidogyne incognita infection.	the root-knot nematode (rkn) meloidogyne incognita reproduces on the roots of tobacco (nicotiana tabacum), damaging crops, reducing crop yield, and causing economic losses annually. the development of resistant genotypes is an alternative strategy to effectively control these losses. however, the molecular mechanism responsible for host pathogenesis and defense responses in tobacco specifically against rkns remain poorly understood. here, root transcriptome analysis of resistant (yuyan12) and su ...	2017	27914810
bioactive volatiles from an endophytic daldinia cf. concentrica isolate affect the viability of the plant parasitic nematode meloidogyne javanica.	plant-parasitic nematodes form one of the largest sources of biotic stress imposed on plants, and are very difficult to control; among them are the obligate parasites, the sedentary root-knot nematodes (rkns)-meloidogyne spp.-which are extremely polyphagous and exploit a very wide range of hosts. endophytic fungi are organisms that spend most of their life cycle within plant tissue without causing visible damage to the host plant. many endophytes secrete specialized metabolites and/or emit volat ...	2016	27997626
comparative efficacy of different approaches to managing meloidogyne incognita on green bean.	a greenhouse study was conducted to compare the relative efficacy of different approaches to managing meloidogyne incognita on green bean. these approaches included chemical (fumigant, non-fumigant, seed dressing, and seed dip), biological (the egg-parasitic fungus, paecilomyces lilacinus and the mycorrhizal fungus glomus sp.), physical (soil solarization), and cultural (chicken litter and urea) methods. accordingly, nine different control materials and application methods plus nematode-infected ...	2017	28053585
mi-1 gene expression in tomato plants under root-knot nematode invasion and treatment with salicylic acid.	the dynamics of expression of two homologous genes mi-1.1 and mi-1.2 in the roots of resistant and susceptible tomato plants in non-invasion conditions and during invasion with the root-knot nematode m. incognita was studied. nematode invasion was accompanied by a significant increase in the expression level of both genes; however, the accumulation of transcripts at the early stages of nematode invasion in the penetration of nematode juveniles to the roots was observed only in plants that contai ...	2016	28058682
selecting native arbuscular mycorrhizal fungi to promote cassava growth and increase yield under field conditions.	the use of arbuscular mycorrhizal fungal (amf) inoculation in sustainable agriculture is now widespread worldwide. although the use of inoculants consisting of native amf is highly recommended as an alternative to commercial ones, there is no strategy to allow the selection of efficient fungal species from natural communities. the objective of this study was (i) to select efficient native amf species (ii) evaluate their impact on nematode and water stresses, and (iii) evaluate their impact on ca ...	2016	28066381
tomato progeny inherit resistance to the nematode meloidogyne javanica linked to plant growth induced by the biocontrol fungus trichoderma atroviride.	root-knot nematodes (rkn) are major crop pathogens worldwide. trichoderma genus fungi are recognized biocontrol agents and a direct activity of trichoderma atroviride (ta) against the rkn meloidogyne javanica (mj), in terms of 42% reduction of number of galls (ng), 60% of number of egg masses and 90% of number of adult nematodes inside the roots, has been observed in tomato grown under greenhouse conditions. an in vivo split-root designed experiment served to demonstrate that ta induces systemic ...	2017	28071749
embryogenesis in the parasitic nematode heterodera glycines is independent of host-derived hatching stimulation.	many parasites regulate their development to synchronize their life cycle with a compatible host. the parasitic nematode heterodera glycines displays incomplete host-mediated hatching behavior wherein some h. glycines individuals hatch only in the presence of a host-derived cue while others hatch in water alone. furthermore, h. glycines shows variable hatching behavior based on oviposition location. the mechanisms regulating this hatching variability are unknown. in this study, we established a ...	2017	28077087
exploiting cell cycle inhibitor genes of the krp family to control root-knot nematode induced feeding sites in plants.	cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the arabidopsis ick/krp members. ectopic krp1, krp2 and krp4 expression resulted in decreased gall size by inhibiting mitotic activity, whereas krp6 induces mitosis in galls. herein, we investigate the role of krp3, krp5 and krp7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. overexpression of krp3 and krp7 culminated i ...	2017	28103637
gene expression analysis in musa acuminata during compatible interactions with meloidogyne incognita.	endoparasitic root-knot nematodes (rkns) (meloidogyne spp.) cause considerable losses in banana (musa spp.), with meloidogyne incognita a predominant species in cavendish sub-group bananas. this study investigates the root transcriptome in musa acuminata genotypes 4297-06 (aa) and cavendish grande naine (cav; aaa) during early compatible interactions with m. incognita methods: roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological ...	2017	28130221
an early record of meloidogyne fallax from ireland.	root-knot nematodes, meloidogyne spp., cause huge economic losses worldwide. currently, three meloidogyne spp. are present on the quarantine a2 list of eppo, meloidogyne chitwoodi, meloidogyne fallax and meloidogyne enterolobii. as a quarantine organism, meloidogyne fallax has been detected in england and northern ireland on sport turf in 2011, and in england on leek in 2013. however, its presence in ireland has probably been overlooked since 1965, when mr. john f. moore and dr. mary t. franklin ...	2017	28144174
below-ground attack by the root knot nematode meloidogyne graminicola predisposes rice to blast disease.	magnaporthe oryzae (rice blast) and the root-knot nematode meloidogyne graminicola are causing two of the most important pathogenic diseases jeopardizing rice production. here, we show that root-knot nematode infestation on rice roots leads to important above-ground changes in plant immunity gene expression, which is correlated with significantly enhanced susceptibility to blast disease. a detailed metabolic analysis of oxidative stress responses and hormonal balances demonstrates that the above ...	2017	28151048
grafting and paladin pic-21 for nematode and weed management in vegetable production.	two years of field trials conducted in a meloidogyne incognita-infested field evaluated grafting and paladin pic-21 (dimethyl disulfide:chloropicrin [dmds:pic] 79:21) for root-knot nematode and weed control in tomato and melon. tomato rootstocks evaluated were; 'tx301', 'multifort', and 'aloha'. 'florida 47' was the scion and the nongrafted control. a double crop of melon was planted into existing beds following tomato harvest. melon rootstocks, c. metulifer and 'tetsukabuto', were evaluated wit ...	2016	28154429
phytoalexin phenalenone derivatives inactivate mosquito larvae and root-knot nematode as type-ii photosensitizer.	phytoalexins phenalenones (pns) are phytochemicals biosynthesized inside the plant in responsive to exterior threat. pns are excellent type-ii photosensitizers, which efficiently produce singlet oxygen upon light irradiation. based on the core functional structure of pns, novel pn derivatives were synthesized here and their singlet oxygen generating abilities and their phototoxicity were evaluated. at the presence of light, these pns have photoinduced toxicity towards aedes albopictus larvae and ...	2017	28169356
genome-wide analysis of excretory/secretory proteins in root-knot nematode, meloidogyne incognita provides potential targets for parasite control.	the root-knot nematode, meloidogyne incognita causes significant damage to various economically important crops. infection is associated with secretion of effector proteins into host cytoplasm and interference with host innate immunity. to combat this infection, the identification and functional annotations of excretory/secretory (es) proteins serve as a key to produce durable control measures. the identification of es proteins through experimental methods are expensive and time consuming while ...	2017	28187376
first record of the root knot nematode, <i>meloidogyne</i> <i>minor</i> in new zealand with description, sequencing information and key to known species of <i>meloidogyne</i> in new zealand.	meloidogyne minor karssen et al. 2004 was collected from perennial ryegrass (lolium perenne l.) growing in a sports ground in christchurch, new zealand. this is a new record for m. minor, the first report of this nematode occurring in new zealand, and the second report from the southern hemisphere (after chile). in general, the new zealand isolate of m. minor corresponds well to the descriptions of m. minor given by karssen et al. (2004). the new zealand isolate is characterized by having a fema ...	2017	28187538
genetic diversity among exotic cotton accessions as for qualitative and quantitative traits.	studying genetic diversity among a group of genotypes is important in genetic breeding because identifying hybrid combinations of greater heterotic effect also increases the chance of obtaining plants with favorable allele combinations in an intra-population selection program. the objective of this study was to compare different types of long and extra-long staple cotton and their genetic diversity in relation to the fiber traits and some agronomic traits in order to grant breeding programs. div ...	2017	28198514
three-dimensional ultrastructure of feeding tubes and interconnected endoplasmic reticulum in root-knot nematode-induced giant cells in rose balsam.	we investigated the three-dimensional ultrastructure of feeding tubes and the surrounding region in giant cells induced in rose balsam (impatiens balsamina l.) roots by the root-knot nematode meloidogyne incognita, using osmium maceration coupled with field emission scanning electron microscopy (fe-sem). in the roots of 35-day-old galled rose balsam plants, adult nematodes induced the formation of giant cells containing feeding tubes and numerous organelles, including tubular endoplasmic reticul ...	2017	28204899
transcriptome analysis of root-knot nematode (meloidogyne incognita)-infected tomato (solanum lycopersicum) roots reveals complex gene expression profiles and metabolic networks of both host and nematode during susceptible and resistance responses.	root knot nematodes (rkns, meloidogyne incognita) are economically important endoparasites having a wide-host range. we have taken a comprehensive transcriptomic approach to investigate the expression of both tomato and rkn genes in tomato roots at five infection time intervals from susceptible plants and two infection time intervals from resistant plants, grown under soil conditions. differentially expressed genes during susceptible (1827-tomato, 462-rkn) and resistance (25-tomato, 160-rkn) int ...	2017	28220591
expression and evolutionary analyses of three acetylcholinesterase genes (mi-ace-1, mi-ace-2, mi-ace-3) in the root-knot nematode meloidogyne incognita.	the full cdna of mi-ace-3 encoding an acetylcholinesterase (ache) in meloidogyne incognita was cloned and characterized. mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. key residues essential to ache structure and function were conserved. the deduced mi-ace-3 protein sequence had 72% amino acid similarity with that of ditylenchus destructor dd-ache-3. phylogenetic analyses using 41 aches from 24 species showed that mi-ace-3 formed a cluster with 4 other nematode a ...	2017	28238686
induction of sa-signaling pathway and ethylene biosynthesis in trichoderma harzianum-treated tomato plants after infection of the root-knot nematode meloidogyne incognita.	salicylic acid-signaling pathway and ethylene biosynthesis were induced in tomato treated with trichoderma harzianum when infected by root-knot nematodes and limited the infection by activation of sar and ethylene production. soil pre-treatment with trichoderma harzianum (th) strains item 908 (t908) and t908-5 decreased susceptibility of tomato to meloidogyne incognita, as assessed by restriction in nematode reproduction and development. the effect of t. harzianum treatments on plant defense was ...	2017	28239746
nematode neuropeptides as transgenic nematicides.	plant parasitic nematodes (ppns) seriously threaten global food security. conventionally an integrated approach to ppn management has relied heavily on carbamate, organophosphate and fumigant nematicides which are now being withdrawn over environmental health and safety concerns. this progressive withdrawal has left a significant shortcoming in our ability to manage these economically important parasites, and highlights the need for novel and robust control methods. nematodes can assimilate exog ...	2017	28241060
genome-wide analysis of expansin superfamily in wild arachis discloses a stress-responsive expansin-like b gene.	expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. the first genome-wide analysis of the expansin superfamily in the arachis genus identified 40 members in a. duranensis and 44 in a. ipaënsis, the wild progenitors of cultivated peanut (a. hypogaea). these expansins were further characterized regarding their subfamily classification, distribution along the genomes, duplication events, molecular structure, a ...	2017	28243841
enhanced synthesis of antioxidant enzymes, defense proteins and leghemoglobin in rhizobium-free cowpea roots after challenging with meloydogine incognita.	the root knot nematodes (rkn), meloydogine spp., particularly meloidogyne incognita and meloidogyne javanica species, parasitize several plant species and are responsible for large annual yield losses all over the world. only a few available chemical nematicides are still authorized for rkn control owing to environmental and health reasons. thus, plant resistance is currently considered the method of choice for controlling rkn, and research performed on the molecular interactions between plants ...	2014	28250394
integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis.	during sampling of several coffea arabica plantations in tanzania severe root galling, caused by a root-knot nematode was observed. from pure cultures, morphology and morphometrics of juveniles and females matched perfectly with meloidogyne africana, whereas morphology of the males matched identically with those of meloidogyne decalineata. based on their cox1 sequence, however, the recovered juveniles, females and males were confirmed to belong to the same species, creating a taxonomic conundrum ...	2017	28257464
insect deterrent and nematicidal screening of microfungi from mexico and anti-aphid compounds from gliomastix masseei.	fungal metabolites are promising alternatives for the development of biorational pesticides. in this sense, microfungi from tropical regions are valuable sources of natural compounds for pest management. with the aim of broadening the search for new eco-friendly products to manage plant pests, this study was carried out to evaluate the biological activity of 23 tropical fungal extracts on three species of phytophagous insects and a plant parasitic nematode. in addition, the active principles of ...	2017	28258772
assessing attraction of nematodes to host roots using pluronic gel medium.	nematodes and other organisms perceive and respond to plant root exudates. these exudates are affected by the condition and genetic makeup of the plant. attraction of the root-knot nematode meloidogyne hapla to roots is altered in plants with mutations affecting ethylene signaling, suggesting that the root exudates to which the nematode responds are modulated by ethylene signaling. nematode interactions with roots have been difficult to observe directly due to the opaqueness of soil. a medium ba ...	2017	28293853
identification of two meloidogyne hapla genes and an investigation of their roles in the plant-nematode interaction.	root-knot nematodes are soil-borne pathogens that invade and establish feeding sites in plant roots. they have an extremely broad host range, including most vascular plants. during infection of a susceptible host, root-knot nematodes secrete molecules called effectors that help them establish an intimate interaction with the plant and, at the same time, allow them to evade or suppress plant immune responses. despite the fact that meloidogyne hapla is a significant pest on several food crops, no ...	2017	28301312
transcriptomic and histological responses of african rice (oryza glaberrima) to meloidogyne graminicola provide new insights into root-knot nematode resistance in monocots.	the root-knot nematode meloidogyne graminicola is responsible for production losses in rice ( oryza sativa ) in asia and latin america. the accession tog5681 of african rice, o. glaberrima , presents improved resistance to several biotic and abiotic factors, including nematodes. the aim of this study was to assess the cytological and molecular mechanisms underlying nematode resistance in this accession.	2017	28334204
new nematotoxic indoloditerpenoid produced by gymnoascus reessii za-130.	chemical investigation of the fungal strain gymnoascus reessii za-130, which was previously isolated from the rhizosphere of tomato plants infected by the root-knot nematode meloidogyne incognita, led to the isolation and identification of a new indoloditerpenoid metabolite designated gymnoascole acetate. its structure was established by spectroscopic methods including 1d- and 2d-nmr and ms analyses. gymnoascole acetate demonstrated strong adverse effects on m. incognita second-stage juvenile (j ...	2017	28343383
multivariate statistics applied to the reaction of common bean plants to parasitism by meloidogyne javanica.	the availability of common bean cultivars tolerant to meloidogyne javanica is limited in brazil. thus, the present study aimed to evaluate the reactions of 33 common bean genotypes (23 landrace, 8 commercial, 1 susceptible standard and 1 resistant standard) to m. javanica, employing multivariate statistics to discriminate the reaction of the genotypes. the experiment was conducted in a greenhouse using a completely randomized design with seven replicates. the seeds were sown in 1-l pots containi ...	2017	28362984
molecular characterization of fmrfamide-like peptides in meloidogyne graminicola and analysis of their knockdown effect on nematode infectivity.	the rice root-knot nematode, meloidogyne graminicola, seriously impairs the growth and yield of rice which is an important staple food worldwide. the disruption of neuropeptide signalling leading to attenuation in nematode behaviour and thereby perturbed infection, offers an attractive alternative to control nematodes. in this direction, the present study was aimed at mining of putative fmrfamide-like peptides (flps) from the transcriptomic dataset of m. graminicola followed by characterization ...	2017	28366833
rapid, simple and direct detection of meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with fta technology.	the northern root-knot nematode (meloidogyne hapla) is a damaging nematode that has caused serious economic losses worldwide. in the present study, a sensitive, simple and rapid method was developed for detection of m. hapla in infested plant roots by combining a flinders technology associates (fta) card with loop-mediated isothermal amplification (lamp). the specific primers of lamp were designed based on the distinction of internal transcribed spacer (its) sequences between m. hapla and other ...	2017	28368036
brassinosteroids act as a positive regulator for resistance against root knot nematode involving respiratory burst oxidase homolog-dependent activation of mapks in tomato.	interplay of hormones with reactive oxygen species (ros) fine-tunes the response of plants to stress, however, the crosstalk between brassinosteroids (brs) and ros in nematode resistance is unclear. in this study, we found that low br biosynthesis or lack of br receptor increased whilst exogenous br decreased the susceptibility of tomato plants to meloidogyne incognita. hormone quantification coupled with hormone mutant complementation experiments revealed that br did not induce the defense resp ...	2017	28370079