Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| inactivation of mycobacterium avium subsp. paratuberculosis in cow's milk by means of high hydrostatic pressure at mild temperatures. | two strains of mycobacterium avium subsp. paratuberculosis (3644/02 and atcc 19698) were inoculated (approximately 6 log cfu/ml) into sterilized milk to evaluate inactivation by high hydrostatic pressure. reductions of m. avium subsp. paratuberculosis increased with pressure level. significant differences were also found between m. avium subsp. paratuberculosis strains and between the media used. average reductions of 4 log cfu/ml after treatment with 500 mpa are comparable to those caused by th ... | 2006 | 16751566 |
| detection of mycobacterium avium subsp. paratuberculosis in bovine manure using whatman fta card technology and lightcycler real-time pcr. | a modified forensic dna extraction and real-time fluorescent polymerase chain reaction assay has been evaluated for the detection of mycobacterium avium subsp. paratuberculosis (map) in bovine fecal samples using primers and fluorescent resonance energy transfer (fret) probes targeting the is900 gene sequence of map. dna was successfully extracted from manure samples by utilizing the whatman fta card technology, which allows for simple processing and storage of samples at room temperature. the f ... | 2006 | 16761949 |
| mycobacterium bovis cultured from commercially pasteurized cows' milk: laboratory cross-contamination. | the ability of mycobacterium paratuberculosis to survive the commercial pasteurization process of raw milk remains controversial. in a study undertaken in venezuela to culture m. paratuberculosis from commercially pasteurized cows' milk, 83-200 ml containers of milk were processed and cultured on herrold's egg yolk slants. no m. paratuberculosis was cultured but a total of six colonies of mycobacterium bovis were isolated from one container each from two different milk providers. because laborat ... | 2006 | 16766143 |
| monensin might protect ontario, canada dairy cows from paratuberculosis milk-elisa positivity. | our objective was to define the role of monensin sodium in protecting cows from being milk-elisa positive for paratuberculosis in ontario, canada dairy herds. in total, 4933 dairy cows from 94 herds were enrolled in this cross-sectional study. forty-four of the enrolled herds were selected purposively by their herd veterinarian and another 50 herds were randomly selected from a local milk production-recording agency. a herd-management survey was completed on each farm during the months of may th ... | 2006 | 16787675 |
| cattle transfers between herds under paratuberculosis surveillance in the netherlands are not random. | the rate and structure of cattle transfers between 206 dutch cattle herds with a 'mycobacterium avium subsp. paratuberculosis (map)-free' status by november 2002, were analyzed over a 3-year period (november 1999-november 2002). of the 206 'map-free' herds, 184 were closed herds during the period studied. in total, 280 cattle had been introduced into 22 herds at an average rate of 0.33 animals per year per 100 cattle present in the 206 herds. assuming a random herd-contact structure, the observe ... | 2006 | 16787676 |
| lateral gene transfer in mycobacterium avium subspecies paratuberculosis. | lateral gene transfer is an integral part of genome evolution in most bacteria. bacteria can readily change the contents of their genomes to increase adaptability to ever-changing surroundings and to generate evolutionary novelty. here, we report instances of lateral gene transfer in mycobacterium avium subsp. paratuberculosis, a pathogenic bacteria that causes johne's disease in cattle. a set of 275 genes are identified that are likely to have been recently acquired by lateral gene transfer. th ... | 2006 | 16788724 |
| use of age and milk production data to improve the ability of enzyme-linked immunosorbent assay test results to predict mycobacterium avium ssp. paratuberculosis fecal culture status. | cows from 2 california dairies were tested for paratuberculosis at the end of lactation by using fecal culture and a commercially available serum enzyme-linked immunosorbent assay (elisa) test kit. individual cow characteristics and production variables were evaluated along with elisa testing results as predictors of fecal culture status. in multivariable logistic regression analysis, age and a herd-standardized version of 305-day mature equivalent (305 me) milk production were significant predi ... | 2006 | 16789710 |
| identification of novel virulence determinants in mycobacterium paratuberculosis by screening a library of insertional mutants. | johne's disease, caused by mycobacterium paratuberculosis infection, is a worldwide problem for the dairy industry and has a possible involvement in crohn's disease in humans. to identify virulence determinants of this economically important pathogen, a library of 5,060 transposon mutants was constructed using tn5367 insertion mutagenesis, followed by large-scale sequencing to identify disrupted genes. in this report, 1,150 mutants were analyzed and 970 unique insertion sites were identified. se ... | 2006 | 16790754 |
| application of a semi-dependent latent model in the bayesian estimation of the sensitivity and specificity of two faecal culture methods for diagnosis of paratuberculosis in sub-clinically infected greek dairy sheep and goats. | in this study, we compared the frequency of isolation of mycobacterium avium subsp. paratuberculosis (map) from faecal samples grown on herrold's egg-yolk medium (heym) or on lowenstein-jensen (lj) medium and estimated the sensitivity (se) and specificity (sp) of the methods separately in sub-clinically infected greek dairy sheep and goats, using latent-class models and bayesian estimation procedures. faecal and blood samples were collected from 400 animals > or =1 year old in april-may 2002. th ... | 2006 | 16797753 |
| bayesian estimation of sensitivity and specificity of serum elisa and faecal culture for diagnosis of paratuberculosis in greek dairy sheep and goats. | latent class models were used to estimate the sensitivity (se) and the specificity (sp) of a serum elisa and a faecal culture (fc) method for the diagnosis of paratuberculosis separately, in sheep and goats. the estimates were obtained by a bayesian method. possible dependence of diagnostic errors was investigated by comparing models where independence was assumed to models allowing for conditional dependence given the true disease status. roc analysis for the serum elisa was also performed and ... | 2006 | 16806541 |
| proposed canadian voluntary national johne's disease prevention and control program. | 2006 | 16808225 | |
| automated high-throughput immunomagnetic separation-pcr for detection of mycobacterium avium subsp. paratuberculosis in bovine milk. | two monoclonal antibody-mediated immunomagnetic separation pcr kits (andiatec paratub-s ims-pcr-elisa and paratub-sl ims-real time pcr) were developed and evaluated for the automated high-throughput detection of mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) in bulk milk of naturally infected dairy herds and made commercially available. m. paratuberculosis are first isolated from milk by high-throughput immunomagnetic bead separation using a completely automated magnetic parti ... | 2006 | 16814891 |
| paratuberculosis and type i diabetes: is this the trigger? | type 1 diabetes mellitus (t1dm) is an autoimmune disease. the etiology of t1dm is incompletely understood but environmental agent(s) are thought to trigger t1dm in the genetically at risk. exposure to cow's milk early in life is a recognized risk factor in the development of t1dm. mycobacterium avium ss. paratuberculosis (map) is the cause of bovine johne's disease and also is thought to act as an immune antigen in crohn's disease and other granulomatous diseases. map is shed in cow's milk and h ... | 2006 | 16828235 |
| does cross-reactivity between mycobacterium avium paratuberculosis and human intestinal antigens characterize crohn's disease? | most crohn's disease (cd) patients show seroreactivity against mycobacterium avium paratuberculosis (map), suggesting a pathogenic role for this organism. our aim was to seek amino acid similarities between map and intestinal proteins that, through molecular mimicry, could serve as targets for cross-reactive immunity in cd. | 2006 | 16831593 |
| in search of desire: mimicry, map, and crohn's disease. | 2006 | 16831612 | |
| interaction between mycobacterium avium subsp. paratuberculosis and environmental protozoa. | interactions between mycobacterium avium subsp. paratuberculosis (map) and free-living protozoa in water are likely to occur in nature. the potential impact of ingestion of map by two naturally occurring acanthamoeba spp. on this pathogen's survival and chlorine resistance was investigated. | 2006 | 16839422 |
| pathology of naturally occurring paratuberculosis in water buffaloes (bubalus bubalis). | gross and histologic lesions of paratuberculosis were studied in water buffaloes. small intestines and associated mesenteric lymph nodes of 405 water buffaloes were examined. of these, 20 animals having visible changes of intestinal thickening, mucosal corrugations, and enlargement of mesenteric lymph nodes exhibited histologic alteration characteristics of mild to moderate granulomatous inflammation. the histologic lesions observed in these animals were classified into 3 grades on the basis of ... | 2006 | 16846987 |
| application of an f57 sequence-based real-time pcr assay for mycobacterium paratuberculosis detection in bulk tank raw milk and slaughtered healthy dairy cows. | a light cycler-based real-time pcr assay that targets the f57 sequence was used to collect data on the prevalence of mycobacterium avium subsp. paratuberculosis (map) in 100 bulk tank raw milk samples and in a population of 101 slaughtered dairy cattle. the assay's reproducible detection limit in total genomic dna templates isolated from 10-ml samples of map-spiked raw milk was 100 cells per ml. similarly, the evaluation of map-spiked bovine feces also demonstrated that the assay had a reproduci ... | 2006 | 16865901 |
| mycobacterium avium subspecies paratuberculosis infection of cattle does not diminish peripheral blood-derived macrophage mycobactericidal activity. | ruminants infected with mycobacterium avium subspecies paratuberculosis consistently develop a multibacillary form of disease that is centered on the ileum. mechanisms responsible for failure of macrophage function during multibacillary disease are incompletely characterized. our data suggest that mycobactericidal functions are present, and potentially enhanced, in monocyte-derived macrophages from m. avium subsp. paratuberculosis infected cattle. addition of cd4(+) t cells from infected animals ... | 2006 | 16884783 |
| peptide amptd-mediated capture pcr for detection of mycobacterium avium subsp. paratuberculosis in bulk milk samples. | a peptide-mediated capture pcr for the detection of mycobacterium avium subsp. paratuberculosis in bulk milk samples was developed and characterized. capture of the organism was performed using peptide amptd, which had been shown to bind to the m. avium subsp. paratuberculosis mptd protein (j. stratmann, b. strommenger, r. goethe, k. dohmann, g. f. gerlach, k. stevenson, l. l. li, q. zhang, v. kapur, and t. j. bull, infect. immun. 72:1265-1274, 2004). consistent expression of the mptd receptor p ... | 2006 | 16885259 |
| a novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens. | a novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. it allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results. | 2006 | 16887225 |
| evaluation of in situ methods used to detect mycobacterium avium subsp. paratuberculosis in samples from patients with crohn's disease. | in common with other diagnostic tests, detection of mycobacteria in tissue by microscopic examination is susceptible to spectrum bias. since crohn's disease is defined by the absence of detectable pathogenic organisms, the use of in situ techniques to search for mycobacterium avium subsp. paratuberculosis in crohn's disease samples requires validation of methods in a paucibacillary setting. to generate paucibacillary infection, c57bl/6 mice were artificially infected with mycobacterium avium sub ... | 2006 | 16891515 |
| short-sequence-repeat analysis of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium isolates collected from animals throughout the united states reveals both stability of loci and extensive diversity. | we analyzed the multilocus short sequence repeats (ssrs) of 211 and 56 isolates of mycobacterium avium subsp. paratuberculosis and m. avium subsp. avium, respectively. the m. avium subsp. paratuberculosis isolates could be differentiated into 61 genotypes. the m. avium subsp. avium isolates showed limited diversity. these ssrs are stable and suitable for studying the molecular epidemiology of m. avium subsp. paratuberculosis. | 2006 | 16891519 |
| a highly sensitive and subspecies-specific surface antigen enzyme- linked immunosorbent assay for diagnosis of johne's disease. | johne's disease (jd), or paratuberculosis, caused by mycobacterium avium subsp. paratuberculosis, is one of the most widespread and economically important diseases of livestock and wild ruminants worldwide. control of jd could be accomplished by diagnosis and good animal husbandry, but this is currently not feasible because commercially available diagnostic tests have low sensitivity levels and are incapable of diagnosing prepatent infections. in this study, a highly sensitive and subspecies-spe ... | 2006 | 16893982 |
| evaluation of the voluntary johne's disease herd status program as a source of replacement cattle. | the objectives of this study were to evaluate the perceived value that enrolled producers gained from participation in the voluntary johne's disease herd status program (vjdhsp), and to evaluate the risk of infection with mycobacterium avium ssp. paratuberculosis (map) of cattle reared in a presumed johne's free environment vs. cattle raised in an environment of unknown johne's status. producers enrolled in level 3 or 4 of the vjdhsp (98 and 99% probability, respectively, of being free from map ... | 2006 | 16899680 |
| heat-treatment of bovine colostrum. ii: effects of heating duration on pathogen viability and immunoglobulin g. | batches (30-l) of first-milking bovine colostrum, inoculated with mycoplasma bovis (10(8) cfu/ml), listeria monocytogenes (10(6) cfu/ml), escherichia coli o157:h7 (10(6) cfu/ml), salmonella enteritidis (10(6) cfu/ml), and mycobacterium avium subsp. paratuberculosis (map; 10(3) cfu/ml), were heat-treated at 60 degrees c for 120 min in a commercial on-farm batch pasteurizer system. duplicate 50-ml subsamples of colostrum were collected at 15-min intervals throughout the heat-treatment process for ... | 2006 | 16899682 |
| use of a polymerase chain reaction to subtype mycobacterium avium subspecies paratuberculosis, an increasingly important pathogen from farmed deer in new zealand. | to review the number of microbiologically-confirmed cases of johne's disease in farmed deer since 2000, and determine the prevalence of the bovine and ovine subtypes of mycobacterium avium subsp paratuberculosis (m. paratuberculosis), using a highly specific polymerase chain reaction (pcr) test on samples from infected herds. | 2006 | 16915342 |
| protein extraction from mycobacterium avium subsp. paratuberculosis: comparison of methods for analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis, native page and surface enhanced laser desorption/ionization time of flight mass spectrometry. | mycobacterium paratuberculosis causes johne's disease, a chronic bowel disease in ruminants worldwide and is currently incurable. this study was conducted to compare methods for examining the proteome of m. paratuberculosis. sds-page, native page and seldi-tof-ms were compared and the efficacy of various lysis buffers was assessed. chaotropic agents (urea chaps and potassium thiocyanate) and non-ionic detergent (tween20 and triton x-100) extracts were compared on three different proteinchip surf ... | 2007 | 16916555 |
| evaluation of a rapid fecal pcr test for detection of mycobacterium avium subsp. paratuberculosis in dairy cattle. | a high-throughput taqman pcr assay for detection of bovine paratuberculosis was evaluated by using fecal samples from 1,808 dairy cattle in seven naturally infected herds and 347 dairy cattle in seven herds considered free of paratuberculosis. fecal, blood, and milk samples were submitted to laboratories where the pcr-based assay, three different fecal culture procedures for mycobacterium avium subsp. paratuberculosis (centrifugation, sedimentation, and the bactec filter concentration method), t ... | 2006 | 16928884 |
| seroprevalences of antibodies against bovine leukemia virus, bovine viral diarrhea virus, mycobacterium avium subspecies paratuberculosis, and neospora caninum in beef and dairy cattle in manitoba. | of 1204 dairy cows and 1425 beef cows sampled, 60.8% and 10.3% were seropositive for bovine leukemia virus, 4.5% and 1.7% were seropositive for mycobacterium avium subspecies paratuberculosis, and 8.3% and 9.1% were seropositive for neospora caninum, respectively, while 28.1% of dairy herds had unvaccinated animals with titres > or = 1:64 for bovine viral diarrhea virus. | 2006 | 16933557 |
| comparison of four different culture media for isolation and growth of type ii and type i/iii mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats. | culture is considered the definitive technique for johne's disease diagnosis, and it is essential for later applications of certain molecular typing techniques. in this study, we have tested four solid media (herrold's egg yolk medium [heym] with sodium pyruvate and mycobactin [heymm-sp], heym with mycobactin and without sodium pyruvate [heymm], middlebrook 7h11 with mycobactin [mm], and löwenstein-jensen with mycobactin [ljm]) for isolation of mycobacterium avium subsp. paratuberculosis strains ... | 2006 | 16957212 |
| identification of diagnostic proteins in mycobacterium avium subspecies paratuberculosis by a whole genome analysis approach. | mycobacterium avium subspecies paratuberculosis (m. paratuberculosis) is an economically significant veterinary pathogen that causes johne's disease in cattle and sheep. there is a critical need for improved diagnostic tests to detect m. paratuberculosis infection in these animals. as with many other animal diseases, efforts need to be concentrated on the development of simple, rapid, noninvasive tests that can be performed by veterinarians or animal producers without expensive laboratory equipm ... | 2006 | 16957356 |
| diagnosis of paratuberculosis in a dairy herd native to brazil. | paratuberculosis (ptb) in brazil has previously only been reported in imported animals and is officially considered as an exotic disease. a dairy herd, which had no imported animals, presented clinically suspect animals and was investigated for paratuberculosis using faecal culture, histopathology, indirect elisa and the agar gel immunodiffusion test. infection with mycobacterium avium subsp. paratuberculosis (map) was confirmed by culture of faeces from five cows with clinical symptoms of ptb a ... | 2007 | 16959504 |
| isolation of high-affinity single-chain antibodies against mycobacterium avium subsp. paratuberculosis surface proteins from sheep with johne's disease. | johne's disease, caused by infection with mycobacterium avium subsp. paratuberculosis, causes significant economic losses to the livestock farming industry. improved investigative and diagnostic tools-necessary to understand disease processes and to identify subclinical infection-are much sought after. here, we describe the production of single-chain antibodies with defined specificity for m. avium subsp. paratuberculosis surface proteins. single-chain antibodies (scfv) were generated from sheep ... | 2006 | 16960114 |
| comparison of bactec 460 and mgit 960 systems for the culture of mycobacterium avium subsp. paratuberculosis s strain and observations on the effect of inclusion of ampicillin in culture media to reduce contamination. | to compare the utility and diagnostic accuracy of bactec and mgit culture systems, a total of 41 pooled faecal samples, each containing faeces from one sheep infected with the s strain of mycobacterium paratuberculosis and four uninfected sheep was cultured. the mgit culture system did not support the growth of the s strain of m. paratuberculosis from faeces within the time frame of the experiments, although a laboratory adapted s strain grew slowly in mgit provided that sufficient bacteria were ... | 2007 | 16971065 |
| mycobacterium avium subsp. paratuberculosis ptpa is an endogenous tyrosine phosphatase secreted during infection. | adaptive gene expression in prokaryotes is mediated by protein kinases and phosphatases. these regulatory proteins mediate phosphorylation of histidine or aspartate in two-component systems and serine/threonine or tyrosine in eukaryotic and eukaryote-like protein kinase systems. the genome sequence of mycobacterium avium subsp. paratuberculosis, the causative agent of johne's disease, does not possess a defined tyrosine kinase. nevertheless, it encodes for protein tyrosine phosphatases. here, we ... | 2006 | 16982836 |
| sampling and repeatability of radiometric faecal culture in bovine johne's disease. | the repeatability of detection of mycobacterium avium subsp. paratuberculosis (map) within and between samplings from 16 paratuberculous dairy cows (13 subclinical; 3 clinical) was investigated by radiometric culture of quadrants of faecal dung pats collected on four to seven occasions over a 10-16-day period. results were compared to serological status and to pathological and bacteriological findings in multiple tissues obtained at slaughter from 15 of the animals 2-6 weeks after the faecal sam ... | 2007 | 16996703 |
| mycobacteria in crohn's disease: a persistent hypothesis. | efforts to explore a mycobacterial origin for crohn's disease typically have involved an epidemiological approach, searching for mycobacterium avium subsp. paratuberculosis in patient tissue. an alternative approach involves consideration of genetic and experimental data regarding host resistance to mycobacteria. from human and mycobacterial genetics, it is known that mycobacterial diseases depend on both pathogen and host factors and that tuberculosis and leprosy are effectively genetic disease ... | 2006 | 17012971 |
| regulation by jun n-terminal kinase/stress activated protein kinase of cytokine expression in mycobacterium avium subsp paratuberculosis-infected bovine monocytes. | to evaluate activation of jun n-terminal kinase/stress-activated protein kinase (jnk/sapk) pathway in bovine monocytes after incubation with mycobacterium avium subsp paratuberculosis (mptb) organisms. | 2006 | 17014329 |
| johne's disease in canada part i: clinical symptoms, pathophysiology, diagnosis, and prevalence in dairy herds. | recent international developments in the area of infectious disease control and nontariff trade barriers, along with possible zoonotic concerns, have provoked a revival of interest in johne's disease in canada and elsewhere. the bacterium causing johne's disease, mycobacterium avium subspecies paratuberculosis, is distributed worldwide and causes chronic granulomatous enteritis, also known as paratuberculosis, in domestic and exotic ruminants, including cattle. the subclinical form of this disea ... | 2006 | 17017652 |
| new pcr systems to confirm real-time pcr detection of mycobacterium avium subsp. paratuberculosis. | johne's disease, a serious chronic form of enteritis in ruminants, is caused by mycobacterium avium subsp. paratuberculosis (map). as the organism is very slow-growing and fastidious, several pcr-based methods for detection have been developed, based mainly on the map-specific gene is900. however, because this gene is similar to genes in other mycobacteria, there is a need for sensitive and reliable methods to confirm the presence of map. as described here, two new real-time pcr systems on the i ... | 2006 | 17020599 |
| identification of proteins of potential diagnostic value for bovine paratuberculosis. | previously we showed that mycobacterium paratuberculosis culture filtrates (cfs) contain more antigens that react with sera from infected cattle than do cellular extracts of the organism. the goal of the present study was to identify proteins of potential diagnostic value among these cf proteins. proteins of potential interest were first separated by 2-de. roughly 240 cf protein spots were detected on cbb-stained gels using phoretix 2d software. of these, 83% reacted with serum from m. paratuber ... | 2006 | 17022102 |
| comparison of the proteosomes and antigenicities of secreted and cellular proteins produced by mycobacterium paratuberculosis. | the protein expression profiles and antigenicities of both culture filtrates (cf) and cellular extracts (ce) of mycobacterium paratuberculosis were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), one-dimensional electrophoresis (1-de) and 2-de immunoblotting, and enzyme-linked immunosorbent assay (elisa). the cf proteins were harvested from supernatants of stationary-phase liquid cultures and concentrated by size exclusion filtration. the ce proteins were extrac ... | 2006 | 17028217 |
| evaluation of environmental sampling and culture to determine mycobacterium avium subspecies paratuberculosis distribution and herd infection status on us dairy operations. | the objectives of this study were to determine the distribution of mycobacterium avium subspecies paratuberculosis (map) in the environment and assess the relationship between the culture status of map in the farm environment and herd infection status. the national animal health monitoring system's dairy 2002 study surveyed dairy operations in 21 states. one component of the study involved collection and culturing of environmental samples for map from areas on farms where manure accumulated from ... | 2006 | 17033002 |
| comparison of milk and serum enzyme-linked immunosorbent assays for diagnosis of mycobacterium avium subspecies paratuberculosis infection in dairy cattle. | milk and serum samples from 35 dairy herds in 17 states were evaluated for cow- and herd-level mycobacterium avium subspecies paratuberculosis (map) antibody test agreement. evaluation of 6,349 samples suggested moderate agreement between milk and serum enzyme-linked immunosorbent assay (elisa) results, with a kappa value of 0.50. cow-level sensitivity (se) for 18 dairy operations with 1,921 animals was evaluated relative to fecal culture results. at the cow level, the milk elisa relative se was ... | 2006 | 17037612 |
| goat paratuberculosis in chile: first isolation and confirmation of mycobacterium avium subspecies paratuberculosis infection in a dairy goat. | in october 2004, 41 goats > 2 years old from a saanen dairy goat herd located in purranque county, 10th region, chile, were sampled and tested for paratuberculosis. while collecting samples it was observed that several goats were thin and emaciated. one goat was sufficiently debilitated to warrant humane euthanasia. this animal was brought to the veterinary school at the universidad austral de chile for necropsy. the goat selected for necropsy was a 12-year-old doe. the animal showed classical c ... | 2006 | 17037618 |
| genomic diversity in mycobacterium avium: single nucleotide polymorphisms between the s and c strains of m. avium subsp. paratuberculosis and with m. a. avium. | following identification of large genomic polymorphisms in a previous study, a polymerase chain reaction and sequencing strategy was used to identify single nucleotide polymorphisms (snps) in 25 genes in the sheep (s) and cattle (c) strains of mycobacterium avium subsp. paratuberculosis (m. a. paratuberculosis) and between m. a. paratuberculosis and m. a. avium. from 12,117 bp of sequence representing 26 loci across 25 genes, 11 snps were identified between the s and c strains in eight genes: hs ... | 2007 | 17049206 |
| herd prevalence of johne's disease. | 2006 | 17056662 | |
| comparative transcriptional analysis of human macrophages exposed to animal and human isolates of mycobacterium avium subspecies paratuberculosis with diverse genotypes. | mycobacterium avium subsp. paratuberculosis is the causative agent of johne's disease in animals and has been hypothesized to be associated with crohn's disease in humans. recently, m. avium subsp. paratuberculosis isolates recovered from crohn's disease patients were shown to have limited diversity, implying the existence of human disease-associated genotypes and strain sharing with animals (a. h. ghadiali et al., j. clin. microbiol. 42:5345-5348, 2004). to explore whether these genotypic diffe ... | 2006 | 17057086 |
| comparison between a commercial and an in-house elisa or anti-m. avium paratuberculosis antibodies detection in dairy herds in rio de janeiro, brazil. | paratuberculosis (johne's disease) is a chronic enteritis that affects ruminants and is caused by mycobacterium avium subsp. paratuberculosis (map). the disease is worldwide spread and causes important economic losses. in brazil, map has already been isolated, but there are no enough epidemiological studies about its prevalence. in this study, 179 sera of dairy cows originated from rio de janeiro state, brazil, were tested for the presence of anti-map antibodies using two elisas. first one was a ... | 2002 | 17061486 |
| gene expression profiling of monocyte-derived macrophages following infection with mycobacterium avium subspecies avium and mycobacterium avium subspecies paratuberculosis. | mycobacterium avium subspecies paratuberculosis (map) and mycobacterium avium subspecies avium (maa) represent two closely related intracellular bacteria with vastly different associated pathologies. maa can cause severe respiratory infections in immune compromised humans but is nonpathogenic in ruminants and is more readily controlled by the bovine immune system than map. map causes a fatal wasting syndrome in ruminants, typified by granulomatous enteritis localized in the small intestine. map ... | 2006 | 17062651 |
| seroprevalence of mycobacterium avium subspecies paratuberculosis, neospora caninum, bovine leukemia virus, and bovine viral diarrhea virus infection among dairy cattle and herds in alberta and agroecological risk factors associated with seropositivity. | a province-wide, cross-sectional seroprevalence and agroecological risk factor study of mycobacterium avium subspecies paratuberculosis (map), neospora caninum (nc), bovine leukemia virus (blv), and bovine viral diarrhea virus (bvdv) genotypes 1 and 2 (bvdv1 and bvdv2) infection in dairy cattle herds in alberta was conducted. among adults, the seroprevalence of map, nc, and blv was 9.1%, 18.5%, and 26.9%, respectively. for map, based on a herd test cutpoint of 2 or more seropositive cows, 58.8% ... | 2006 | 17078247 |
| rapid expression of mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery. | mycobacterium avium subsp. paratuberculosis is the causative agent of johne's disease, a chronic granulomatous enteritis of ruminants and other species. detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. we describe here an approach that utilizes translationally active pcr fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in m. avium subsp. paratuberculosis. the investigations showed th ... | 2007 | 17079432 |
| comparative evaluation of positive tests to mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west greece using molecular techniques, serology, and culture. | mycobacterium avium subsp. paratuberculosis (map) is the cause of paratuberculosis, which affects mainly ruminants although there is a growing concern about its possible implication in crohn's disease in humans especially in connection with environmental spread and risks to the food chain. retail cheese may represent a significant source of human exposure to map and the aim of this study was to assess map status in clinically healthy sheep and goats in greece, comparing techniques routinely used ... | 2007 | 17084095 |
| detection of mycobacterium avium subsp. paratuberculosis in two brown bears in the central european carpathians. | the incidence of mycobacterial infections was monitored in brown bears (ursus arctos) in the national park low tatras in the central european carpathians in slovakia. tissue samples of 20 brown bears were examined microscopically and by culture for the presence of mycobacteria. acid-fast rods were detected by ziehl-neelsen staining in a smear from the kidney of one brown bear, although the culture was negative for mycobacteria. mycobacterium avium subsp. paratuberculosis, the causative agent of ... | 2006 | 17092904 |
| validation of endogenous reference genes for expression profiling of raw264.7 cells infected with mycobacterium avium subsp. paratuberculosis by quantitative pcr. | reference genes are frequently used to normalize between different biological samples the levels of mrna measured using quantitative pcr (qpcr). the expression level of many commonly used reference genes has been shown to vary between tissues or cells, or following exposure to various treatments including infection with microbes. the selection of an appropriate reference gene for an individual experiment is therefore a crucial step in the process of accurately determining changes in gene express ... | 2007 | 17097151 |
| simulated economic effects of improving the sensitivity of a diagnostic test in paratuberculosis control. | low sensitivity (se) of diagnostic tools is often mentioned as a major problem in the control of paratuberculosis (ptb) and much effort is put into the improvement of these tests. the hypothetical perspectives of improving the se of a milk-antibody elisa (hereafter: milk-elisa) used in test-&-cull strategies against ptb in dairy cattle were investigated by simulations. the current se varies between 10 and 80%, increasing with increasing lactation stage, parity and infection stage. we simulated t ... | 2007 | 17101188 |
| age at occurrence of mycobacterium avium subspecies paratuberculosis in naturally infected dairy cows. | paratuberculosis is a chronic infection of ruminants and other species caused by mycobacterium avium ssp. paratuberculosis (map). establishing test strategies for paratuberculosis will require insight into the temporal aspects of certainty with a given test. in this study, the age at which cows tested positive by elisa and fecal culture (fc) was investigated by use of time-to-event analyses. the effects of herd, parity, and shedding group were evaluated at the age of test-positive elisa and fc, ... | 2006 | 17106087 |
| analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed elisa to field sample testing in korea. | paratuberculosis (ptb) is a major disease problem worldwide, and causes major economic losses in the dairy industry. although ptb has been reported in korea, no studies have been conducted to determine its prevalence and no program has been developed to control the disease. in this study, the sera of beef (n = 1,056) and dairy cattle (n = 1,105) from all provinces in korea were tested to determine the prevalence of ptb using two different elisa: an 'in house' modified absorbed elisa (p-elisa) ba ... | 2006 | 17106226 |
| "in vitro" activities of antimycobacterial agents against mycobacterium avium subsp. paratuberculosis linked to crohn's disease and paratuberculosis. | 2006 | 17107610 | |
| the association between mycobacterium avium subsp. paratuberculosis fecal shedding or clinical johne's disease and lactation performance on two minnesota, usa dairy farms. | lactation performance of cows infected with mycobacterium avium subsp. paratuberculosis (map) was previously studied using only serum elisa as a diagnostic method. this study evaluated on two dairy farms in minnesota, usa the lactation performance (measures of health, production, reproduction, and survival) of cows shedding map in feces before calving and of cows culled with clinical signs consistent with johne's disease (jd) during the subsequent lactation. fecal samples were collected from 105 ... | 2007 | 17118473 |
| a testing scheme for the detection of mycobacterium avium subsp. paratuberculosis in bovine feces utilizing the esp para-jem liquid culture system. | a testing scheme for the detection of mycobacterium avium subsp. paratuberculosis (map) in broth cultures of bovine fecal samples carried out in esp para-jem system was evaluated. the scheme included acid-fast staining (on signal-positive and signal-negative samples), and confirmation by pcr for 2 map-specific targets and subculture of all acid-fast positive pcr-negative samples. two hundred and fifty bovine fecal samples were evaluated for the presence of map using this scheme. thirty-seven (15 ... | 2006 | 17121079 |
| comparison of three dna preparation methods for real-time polymerase chain reaction confirmation of mycobacterium avium subsp. paratuberculosis growth in an automated broth culture system. | three methods of harvesting dna from broth culture tubes for quantitative real-time polymerase chain reaction (qrtpcr) confirmation of mycobacterium avium subsp. paratuberculosis (map) were evaluated. a commercial dna extraction kit, the boil method (boiling for 5 minutes), or direct addition of broth culture media to the pcr reaction mix were tested. samples were evaluated at 8 or 11 days of incubation and at the time of instrument-signal culture-positive. in total, when tested at time to instr ... | 2006 | 17121088 |
| serological, bacteriological and molecularbiological survey of paratuberculosis (johne's disease) in austrian cattle. | paratuberculosis (johne's disease) is a chronic infectious disease of ruminants, caused by mycobacterium avium subspecies paratuberculosis (map). because of its long incubation period, high economic losses, difficulties in diagnosis and possible links to morbus crohn in humans, paratuberculosis is one of the most important diseases of ruminants today. an abattoir-based nationwide survey on the occurrence of map in the austrian cattle population was performed using serology (svanovir-elisa) as we ... | 2006 | 17123426 |
| efficacy of spheroplastic and cell-wall competent vaccines for mycobacterium avium subsp. paratuberculosis in experimentally-challenged baby goats. | a mycobacterium avium subspecies paratuberculosis (map) vaccine that reduced the incidence of clinical disease or reduced fecal shedding of map would aid control of johne's disease (jd). the objective of the present study was to evaluate the efficacy of four map vaccine combinations, including cell-wall competent (cwc) alum adjuvant, cwc-qs21 adjuvant, cell-wall deficient (cwd) alum adjuvant and cwd-qs21 adjuvant vaccines. eighty baby goats were vaccinated at 1 and 4 weeks of age with one of the ... | 2007 | 17123751 |
| gut microbes, the innate immune system and inflammatory bowel disease: location, location, location. | 2007 | 17133076 | |
| proteome and differential expression analysis of membrane and cytosolic proteins from mycobacterium avium subsp. paratuberculosis strains k-10 and 187. | little is known of protein expression in mycobacterium avium subsp. paratuberculosis and how this contributes to pathogenesis. in the present study, proteins from both membranes and cytosol were prepared from two strains of m. avium subsp. paratuberculosis, i.e., laboratory-adapted strain k-10 and a recent isolate, strain 187, obtained from a cow exhibiting clinical signs of johne's disease. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cytosol and membrane proteins from k-10 and ... | 2007 | 17142399 |
| monitoring responses by use of five-color flow cytometry in subsets of peripheral t cells obtained from cattle inoculated with a killed mycobacterium avium subsp paratuberculosis vaccine. | to monitor by use of 5-color flow cytometry the antigen-specific responses of subsets of peripheral t cells in cattle inoculated with a killed mycobacterium avium subsp paratuberculosis (map) vaccine and to compare results with those for 2 established cell-mediated immunity assays. | 2006 | 17144810 |
| johne's disease in canada part ii: disease impacts, risk factors, and control programs for dairy producers. | part i of this 2-part review examined the clinical stages, pathophysiology, diagnosis, and epidemiology of johne's disease, providing information relevant to canada, where available. in part ii, a critical review of the economic impacts of the disease, risk factors, and important control measures are presented to enable canadian bovine practitioners to successfully implement control strategies and participate in control programs. in cattle positive by enzyme-linked immunosorbant assay, there is ... | 2006 | 17147140 |
| proper estimation of sensitivity and specificity. | 2006 | 17151187 | |
| proteomic comparison of mycobacterium avium subspecies paratuberculosis grown in vitro and isolated from clinical cases of ovine paratuberculosis. | paratuberculosis (johne's disease) poses a significant economic problem to beef, dairy and sheep industries worldwide, and is caused by mycobacterium avium subspecies paratuberculosis. in this study, 2d page was used as a tool to investigate the virulent state of m. avium subsp. paratuberculosis, incorporating the technique of beating the organism with zirconium/silica beads to provide a comprehensive representation of its proteome. a direct comparison of the proteomes of m. avium subsp. paratub ... | 2007 | 17185548 |
| bayesian analysis to validate a commercial elisa to detect paratuberculosis in dairy herds of southern chile. | in chile, mycobacterium avium subsp. paratuberculosis (map) has been isolated on several occasions and clinical cases have been reported. nevertheless, diagnostic tests have not yet been validated for this agent in the chilean setting. the objective of the study was to validate a commercial elisa to detect map shedding dairy cows in management conditions, prevalence and stages of infection existing in southern chile, utilising different statistical approaches. blood and faeces were collected fro ... | 2007 | 17188381 |
| modified culture protocol for isolation of mycobacterium avium subsp. paratuberculosis from raw milk. | a modified culture method using c18-carboxypropylbetaine (cb-18) and microscopic screening was evaluated for time to and limit of detection of mycobacterium avium subsp. paratuberculosis (map) in raw milk. bulk-tank milk samples were spiked with six different concentrations (10(1) to 10(6) cfu/ml) of map. samples were processed using two different protocols. the first protocol involved specimen processing with the zwitterionic detergent c18-carboxypropylbetaine (cb-18) and lytic enzymes followed ... | 2006 | 17199528 |
| development of elisa to detect antibodies specific to mycobacterium avium subsp. paratuberculosis with truncated 34 kda proteins. | to develop elisa to detect antibodies specific to mycobacterium avium subsp. paratuberculosis (m. paratuberculosis), the carboxyl termini of the 34 kda proteins of m. paratuberculosis and mycobacterium avium subsp. avium (m. avium) were expressed in escherichia coli expression system. antibodies specific to m. paratuberculosis were detected with the truncated 34 kda protein of m. paratuberculosis in elisa after pre-absorption of serum samples with the truncated 34 kda protein of m. avium. all th ... | 2006 | 17201195 |
| seroprevalence of mycobacterium avium subspecies paratuberculosis in korean black goats (capra hircus aegagrus). | in total, 582 sera from 116 black goat herds were analyzed by a commercially available elisa kit to monitor the seroprevalence of mycobacterium avium subspecies paratuberculosis (mpt) in korean black goats (capra hircus aegagrus). the mean number of goats sampled per herd was 5.11, 4.66, and 5.38 for the northern, central, and southern regions of korea, respectively. the apparent regional prevalence of mpt was estimated at 18.2-38.2% and 4.6-15.3% for herds and goats, respectively. the mpt-posit ... | 2006 | 17213715 |
| a case-control study of drinking water and dairy products in crohn's disease--further investigation of the possible role of mycobacterium avium paratuberculosis. | similarities between johne's disease in ruminants and crohn's disease in humans have led to speculation that mycobacterium avium paratuberculosis (map) might be a causative agent in crohn's disease. however, evidence remains inconsistent. in this case-control study (1999-2004), the authors assessed the possible role of drinking water and dairy products potentially contaminated with map in the etiology of crohn's disease. a total of 218 patients with crohn's disease recruited from nine hospitals ... | 2007 | 17237136 |
| testing the interaction between nod-2 status and serological response to mycobacterium paratuberculosis in cases of inflammatory bowel disease. | in a population-based case-control study we have previously shown that 14% of healthy manitobans carry one or two mutations in the nod-2 locus, a gene highly associated with crohn's disease (cd). the nod-2 protein is the receptor responsible for recognition of bacterial peptidoglycans, and it is plausible that nod-2 is involved in the recognition of mycobacteria. thirty-seven percent of manitobans with cd had >or=1 nod-2 mutation, leading to a threefold increased risk of cd for single-mutant car ... | 2007 | 17251406 |
| on the action of methotrexate and 6-mercaptopurine on m. avium subspecies paratuberculosis. | clinical improvement in inflammatory bowel disease (ibd) treated with methotrexate and 6-mercaptopurine (6-mp) is associated with a decrease in pro-inflammatory cytokines. this has been presumed to indicate the mechanism of action of methotrexate and 6-mp. although controversial, there are increasingly compelling data that mycobacterium avium subspecies paratuberculosis (map) may be an etiological agent in some or all of ibd. we hypothesized that the clinical efficacy of methotrexate and 6-mp in ... | 2007 | 17252054 |
| mycobacterium avium subspecies paratuberculosis and mycobacterium avium subsp. avium infections in a tule elk (cervus elaphus nannodes) herd. | between 2 august and 22 september 2000, 37 hunter-killed tule elk (cervus elaphus nannodes) were evaluated at the grizzly island wildlife area, california, usa, for evidence of paratuberculosis. elk were examined post-mortem, and tissue and fecal samples were submitted for radiometric mycobacterial culture. acid-fast isolates were identified by a multiplex polymerase chain reaction (pcr) that discriminates among members of the mycobacterium avium complex (mac). histopathologic evaluations were c ... | 2006 | 17255437 |
| infections caused by mycobacterium avium subspecies avium, hominissuis, and paratuberculosis in free-ranging red deer (cervus elaphus hippelaphus) in austria, 2001-2004. | between 2001 and 2004, 14 austrian free-ranging red deer (cervus elaphus hippelaphus) infected by mycobacterium avium species were observed. eight of the cases were from different geographical regions, and six originated from the same hunting area. the affected animals had signs of diarrhea, severe weight loss, and emaciation. on post-mortem examination, lymphadenitis associated with grossly enlarged mesenteric lymph nodes as well as multiple caseous or purulent nodular lesions in the thickened ... | 2006 | 17255438 |
| optimization of methods for detecting mycobacterium avium subsp. paratuberculosis in environmental samples using quantitative, real-time pcr. | detection of johne's disease, an enteric infection of cattle caused by mycobacterium avium subsp. paratuberculosis (m. paratuberculosis), has been impeded by the lack of rapid, reliable detection methods. the goal of this study was to optimize methodologies for detecting m. paratuberculosis in manure from an infected dairy cow or in contaminated soil samples using a quantitative, real-time pcr (qrt-pcr) based analysis. three different nucleic acid extraction techniques, the efficiency of direct ... | 2007 | 17257697 |
| development of a new, combined rapid method using phage and pcr for detection and identification of viable mycobacterium paratuberculosis bacteria within 48 hours. | the fastplaquetb assay is an established diagnostic aid for the rapid detection of mycobacterium tuberculosis from human sputum samples. using the fastplaquetb assay reagents, viable mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. the bacteriophage used does not infect m. avium subsp. paratuberculosis alone, so to add specificity to this assay, a pcr-based identification method was introduced to amplify m. avium subsp. paratuberculosis-specific sequ ... | 2007 | 17259362 |
| production and characterization of monoclonal antibodies against a major membrane protein of mycobacterium avium subsp. paratuberculosis. | the mycobacterium avium subsp. paratuberculosis 35-kda major membrane protein (mmp) encoded by map2121c is an important membrane antigen recognized in cattle with johne's disease. in this study, purified recombinant mmp was used to produce two stable monoclonal antibodies, termed 8g2 and 13e1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy. | 2007 | 17267586 |
| failure of antigen-stimulated gammadelta t cells and cd4+ t cells from sensitized cattle to upregulate nitric oxide and mycobactericidal activity of autologous mycobacterium avium subsp. paratuberculosis-infected macrophages. | the function of gammadelta t cells during ruminant paratuberculosis (johne's disease) is presently unknown. an ex vivo system was used to test the hypothesis that gammadelta t cells are capable of activating mycobacterium avium subsp. paratuberculosis-(m. paratuberculosis)-infected macrophages. peripheral blood-derived macrophages were infected in vitro with live m. paratuberculosis, and autologous ln-derived gammadelta t cells or cd4+ t cells were co-cultured with infected macrophages for 48h, ... | 2007 | 17275098 |
| antibody and skin-test responses of sheep vaccinated against johne's disease. | current vaccines against mycobacterium avium subsp. paratuberculosis (map, johne's disease) may cause animals to react positively when tested for mycobacterium bovis (bovis). therefore, the effects of vaccination on map serum ab and skin-test responses to map and bovis ppd were compared in 25 ewes vaccinated against map with 24 control ewes in an infected flock 3 years post-vaccination. map-specific ab levels were higher (p<0.001) in vaccinated ewes than in control ewes. all increases in skinfol ... | 2007 | 17275919 |
| a long-term bacteriological and immunological study in holstein-friesian cattle experimentally infected with mycobacterium avium subsp. paratuberculosis and necropsy culture results for holstein-friesian cattle, merino sheep and angora goats. | the aims were to longitudinally evaluate the interferon-gamma (ifn-gamma) test in comparison to faecal culture and the absorbed elisa in a cattle infection model for johne's disease and to determine the adult infection status, by necropsy and tissue culture, of sheep, goats and cattle infected as young animals. clinical disease, faecal culture results and immunological responses for merino sheep [stewart, d.j., vaughan, j.a., stiles, p.l., noske, p.j., tizard, m.l.v., prowse, s.j., michalski, w. ... | 2007 | 17289303 |
| [the suitability of elisa for the detection of antibodies against mycobacterium avium ssp. paratuberculosis in bulk milk samples from rhineland-palatinate]. | paratuberculosis or johne's disease, caused by mycobacterium avium subspecies paratuberculosis (map), is a notifiable disease in germany which produces enormous economical losses in dairy farms. at present,there is no confirmed data about the actual number of infected livestock herds in germany. a countrywide monitoring program to evaluate the prevalence in dairy herds would only be economically feasible on the basis of bulk milk testing. in this study, we evaluated two elisa test kits (svanovir ... | 2007 | 17290945 |
| genome and transcriptome scale portrait of sigma factors in mycobacterium avium subsp. paratuberculosis. | mycobacterium avium subsp. paratuberculosis (map) is the etiological agent of johne's disease (jd), a chronic gastroenteritis of ruminants and other animals, including primates. many evidences suggested association of map to crohn's disease, a chronic granulomatous gastrointestinal disease of humans with strong similarities with jd. the present study attempts to evaluate global gene regulation in map, which has not been addressed previously, despite the availability of map genome sequence. for t ... | 2007 | 17292677 |
| cloning, expression, purification and serodiagnostic evaluation of fourteen mycobacterium paratuberculosis proteins. | fourteen proteins of potential diagnostic value for bovine paratuberculosis were identified in the culture filtrate of mycobacterium paratuberculosis jtc303 by immunoblot and mass spectrometry. the goals of the present study were to express these 14 orfs in escherichia coli and evaluate their antigenicity. all 14 proteins were expressed in e. coli bl21(de3) after transformation with the pet-22b(+) vector. yields of insoluble proteins were higher than those of the soluble proteins. polyclonal rab ... | 2007 | 17296312 |
| invasion and persistence of mycobacterium avium subsp. paratuberculosis during early stages of johne's disease in calves. | infection with mycobacterium avium subsp. paratuberculosis causes johne's disease in cattle and is a serious problem for the dairy industry worldwide. development of models to mimic aspects of johne's disease remains an elusive goal because of the chronic nature of the disease. in this report, we describe a surgical approach employed to characterize the very early stages of infection of calves with m. avium subsp. paratuberculosis. to our surprise, strains of m. avium subsp. paratuberculosis wer ... | 2007 | 17296749 |
| one-step, nanoparticle-mediated bacterial detection with magnetic relaxation. | acknowledging the need for the development of fast and sensitive bacterial detection methods, we functionalized superparamagnetic iron oxide nanoparticles to identify mycobacterium avium spp. paratuberculosis (map), through magnetic relaxation. our results indicate that the map nanoprobes (1) bind specifically to map, (2) can quantify the bacterial target quickly in milk and blood with high sensitivity, and (3) are not susceptible to interferences caused by other bacteria. the use of the describ ... | 2007 | 17298004 |
| improved template dna preparation procedure for detection of mycobacterium avium subsp. paratuberculosis in milk by pcr. | factors affecting the detection of mycobacterium avium subsp. paratuberculosis (map) by pcr in raw milk and their interactions were investigated. three day old bulk tank raw milk (50 ml) samples were seeded with map at a level of an estimated 30 cfu/ml. heat-treatment of raw milk before centrifugation significantly affected the partitioning of map in the cream, whey and pellet fractions. based on the is900 pcr results, map preferentially partitioned into the cream fraction in unheated raw milk, ... | 2007 | 17307264 |
| horizontal transmission of mycobacterium avium subsp. paratuberculosis in cattle in an experimental setting: calves can transmit the infection to other calves. | in september 2001, two subsequent transmission experiments both lasting 3 months were carried out to study cow-calf transmission of mycobacterium avium subsp. paratuberculosis (map) (period 1), followed by calf-calf transmission of the infection (period 2). every 2 weeks, serum, heparinised blood and faecal samples were collected from all animals. after these experiments, the 20 calves were housed individually for more than 3 years to be able to detect the infection status and excretion pattern ... | 2007 | 17317041 |
| evaluation of highly sensitive indigenous milk elisa kit with fecal culture, milk culture and fecal-pcr for the diagnosis of bovine johne's disease (bjd) in india. | country lacks indigenous diagnostic kits against johne's disease in animals. indigenous elisa and is 900 pcr kits, originally developed for goats and sheep, have been adapted for screening of lactating cows. multiple diagnostic tests were used to screen 26 lactating dairy cows against johne's disease. milk elisa was evaluated with fecal culture, milk culture and fecal pcr. of the 26 samples from lactating cows, 84.6, 96.1, 88.4 and 23.0% were positive in fecal culture, milk culture, m-elisa and ... | 2007 | 17328952 |
| relationship between mycobacterium avium subspecies paratuberculosis, il-1alpha, and traf1 in primary bovine monocyte-derived macrophages. | mycobacterium avium subspecies paratuberculosis (map) is a facultative intracellular pathogen that resides in host macrophage cells. presently, little is known about how map is able to subvert the normal bacteriocidal functions of infected macrophages. previously, we reported that ileal tissues from map infected cattle contained high levels of interleukin-1 alpha (il-1alpha) and tumor necrosis factor receptor-associated factor 1 (traf1), relative to ileal tissues from uninfected cattle. high-lev ... | 2007 | 17328964 |
| a dna sequence capture extraction method for detection of mycobacterium avium subspecies paratuberculosis in feces and tissue samples. | culturing of mycobacterium avium subspecies paratuberculosis (map) remains difficult and is time consuming. an alternative for the rapid detection of map in samples is pcr. we have developed a sensitive dna-extraction method based on sequence capture for the rapid detection of m. avium subspecies paratuberculosis by pcr in fecal and tissue samples. the method detected 10(2)map/g feces using spiked samples, and reached a diagnostic sensitivity of 33,7% compared to 22% for culture. analysis of tis ... | 2007 | 17329043 |
| field testing of an enhanced direct-fecal polymerase chain reaction procedure, bacterial culture of feces, and a serum enzyme-linked immunosorbent assay for detecting mycobacterium avium subsp paratuberculosis infection in adult dairy cattle. | to estimate the sensitivity (se) and specificity (sp) for an enhanced direct-fecal pcr procedure, bacterial culture of feces (bcf), and a serum elisa for detecting mycobacterium avium subsp paratuberculosis (map) infection in adult dairy cattle. | 2007 | 17331011 |
| successful treatment of a crohn's disease patient infected with bacteremic mycobacterium paratuberculosis. | 2007 | 17335456 | |
| elisa and pcr for evaluation of subclinical paratuberculosis in the saudi dairy herds. | 2007 | 17339085 | |
| experimental infections in young red deer (cervus elaphus) with a bovine and an ovine strain of mycobacterium avium subsp paratuberculosis. | to compare the virulence of a 'bovine' and an 'ovine' strain of mycobacterium avium subsp paratuberculosis (m. ptb) in red deer (cervus elaphus) after experimental inoculation orally, and to examine the relationship between the dose of the bovine strain given and immunological, clinical and histopathological outcomes in young red deer. | 2007 | 17339913 |