Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| analysis of 16s libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. | total genomic dna from samples of intact mouse small intestine, large intestine, caecum and faeces was used as template for pcr amplification of 16s rrna gene sequences with conserved bacterial primers. phylogenetic analysis of the amplification products revealed 40 unique 16s rdna sequences. of these sequences, 25% (10/40) corresponded to described intestinal organisms of the mouse, including lactobacillus spp., helicobacter spp., segmented filamentous bacteria and members of the altered schaed ... | 2002 | 12427955 |
| microscopic and ultrastructural lesions of the ureter and renal pelvis in sows with regard to actinobaculum suis infection. | tissues from ureter and renal pelvis of 18 sows naturally (n = 15) and experimentally (n = 3) infected with actinobaculum suis (former actinomyces, eubacterium suis) were studied using light and scanning as well as transmission electron microscopy. the results were compared with the findings from 11 clinically healthy sows as controls. the lesions in both the ureter and renal pelvis of naturally and experimentally infected animals were similar. in severe cases there were necrotizing ureteritis a ... | 2002 | 12440789 |
| rotation of the exo-methylene group of (r)-3-methylitaconate catalyzed by coenzyme b(12)-dependent 2-methyleneglutarate mutase from eubacterium barkeri. | 2-methyleneglutarate mutase from the anaerobe eubacterium (clostridium) barkeri is an adenosylcobalamin (coenzyme b(12))-dependent enzyme that catalyzes the equilibration of 2-methyleneglutarate with (r)-3-methylitaconate. two possibilities for the mechanism of the carbon skeleton rearrangement of the substrate-derived radical to the product-related radical are considered. in both mechanisms an acrylate group migrates from c-3 of 2-methyleneglutarate to c-4. in the "addition-elimination" mechani ... | 2002 | 12440902 |
| evolutionary analyses of the small subunit of glutamate synthase: gene order conservation, gene fusions, and prokaryote-to-eukaryote lateral gene transfers. | lateral gene transfer has been identified as an important mode of genome evolution within prokaryotes. except for the special case of gene transfer from organelle genomes to the eukaryotic nucleus, only a few cases of lateral gene transfer involving eukaryotes have been described. here we present phylogenetic and gene order analyses on the small subunit of glutamate synthase (encoded by gltd) and its homologues, including the large subunit of sulfide dehydrogenase (encoded by suda). the scattere ... | 2002 | 12455964 |
| evolutionarily different rna motifs and rna-protein complexes to achieve selenoprotein synthesis. | a wealth of rnas or rna motifs are instrumental in controlling a variety of post-transcriptional or post-translational regulations. in this regard, selenocysteine incorporation in response to a redefined uga stop codon certainly constitutes an intriguing and fascinating process. translation elongation factors specialized for selenocysteine are needed to decode uga selenocysteine codons. discrimination between uga selenocysteine and uga stop codons also necessitates selenoprotein mrna hairpins, c ... | 2002 | 12457564 |
| three-dimensional structure of manganese superoxide dismutase from bacillus halodenitrificans, a component of the so-called "green protein". | a so-called "green protein" has been purified from a moderate halophilic eubacterium, bacillus halodenitrificans (atcc 49067), under anaerobic conditions. the protein, which might play an important role in denitrification, dissociates mainly into two components after exposure to air: a manganese superoxide dismutase (gp-mnsod) and a nucleoside diphosphate kinase. as a first step in elucidating the overall structure of the green protein and the role of each component, the 2.8-a resolution crystal ... | 2002 | 12457847 |
| catalysis at a dinuclear [cusmo(==o)oh] cluster in a co dehydrogenase resolved at 1.1-a resolution. | the co dehydrogenase of the eubacterium oligotropha carboxidovorans is a 277-kda mo- and cu-containing iron-sulfur flavoprotein. here, the enzyme's active site in the oxidized or reduced state, after inactivation with potassium cyanide or with n-butylisocyanide bound to the active site, has been reinvestigated by multiple wavelength anomalous dispersion measurements at atomic resolution, electron spin resonance spectroscopy, and chemical analyses. we present evidence for a dinuclear heterometal ... | 2002 | 12475995 |
| development of a membrane-array method for the detection of human intestinal bacteria in fecal samples. | a membrane-array method was developed for the detection of human intestinal bacteria in fecal samples without using the expensive microarray-arrayer and laser-scanner. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to nitrocellulose membranes. digoxigenin (dig)-labeled 16s rdnas were amplified by polymerase chain ... | 2002 | 12477438 |
| the microbiology of butyrate formation in the human colon. | butyrate arising from microbial fermentation is important for the energy metabolism and normal development of colonic epithelial cells and has a mainly protective role in relation to colonic disease. while certain dietary substrates such as resistant starch appear to be butyrogenic in the colon, it is not known to what extent these stimulate butyrate production directly, e.g. by promoting amylolytic species, or indirectly, e.g. through cross-feeding of fermentation products. cultural and molecul ... | 2002 | 12480096 |
| lytb protein catalyzes the terminal step of the 2-c-methyl-d-erythritol-4-phosphate pathway of isoprenoid biosynthesis. | recombinant lytb protein from the thermophilic eubacterium aquifex aeolicus produced in escherichia coli was purified to apparent homogeneity. the purified lytb protein catalyzed the reduction of (e)-4-hydroxy-3-methyl-but-2-enyl diphosphate (hmbpp) in a defined in vitro system. the reaction products were identified as isopentenyl diphosphate and dimethylallyl diphosphate. a spectrophotometric assay was established to determine the steady-state kinetic parameters of lytb protein. the maximal spe ... | 2002 | 12482608 |
| presence of several cellulose-binding proteins in culture supernatant and cell lysate of eubacterium cellulosolvens 5. | attempts were made to separate and characterize cellulose-binding proteins (cbps) from both the culture supernatant and cell lysate of eubacterium cellulosolvens 5. once the cbps were bound to avicel cellulose, they were then effectively eluted with the solution containing 3.2 or 5% sodium dodecyl sulfate (sds), but not eluted with the solution containing various kinds of carbohydrates and reagents. namely, cbps in both the culture supernatant and cell lysate of the bacterium bound tightly and s ... | 2001 | 12483607 |
| lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep. | condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. however, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. the aim of this study was to investigate the specific effects of condensed tannins from lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (lolium perenne ... | 2002 | 12489781 |
| in vitro activities of daptomycin, vancomycin, quinupristin- dalfopristin, linezolid, and five other antimicrobials against 307 gram-positive anaerobic and 31 corynebacterium clinical isolates. | the activities of daptomycin, a cyclic lipopeptide, and eight other agents were determined against 338 strains of gram-positive anaerobic bacteria and corynebacteria by the nccls reference agar dilution method with supplemented brucella agar for the anaerobes and mueller-hinton agar for the corynebacteria. the daptomycin mics determined on ca(2+)-supplemented (50 mg/liter) brucella agar plates were one- to fourfold lower than those determined in unsupplemented media. daptomycin was highly active ... | 2003 | 12499210 |
| preliminary x-ray crystallographic analysis of trna pseudouridine 55 synthase from the thermophilic eubacterium thermotoga maritima. | thermotoga maritima trub, an enzyme responsible for the formation of pseudouridine in trna, has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mm citrate ph 3.5, 200 mm li(2)so(4), 20% glycerol, 13% peg 8000. crystals display orthorhombic symmetry, with unit-cell parameters a = 47.39, b = 83.88, c = 98.72 a, and diffract to 2.0 a resolution using synchrotron radiation. a solution was obtained by molecular replacement using part of the recently published crystal ... | 2003 | 12499554 |
| cloning, expression, and efficient purification in escherichia coli of a halophilic nucleoside diphosphate kinase from the moderate halophile halomonas sp. #593. | most typical halophilic enzymes from extremely halophilic archaea require high concentrations of salt for their activity and stability. these enzymes are inactive in escherichia coli unless refolded in the presence of salts in vitro. in this report, we describe cloning of the ndk gene of nucleoside diphosphate kinase from a moderately halophilic eubacterium and overexpression of the protein in e. coli as an n-terminal hexa-his fusion to facilitate its purification on ni-nta affinity resin. we de ... | 2003 | 12509994 |
| community analysis of nitrifying bacteria in an advanced and compact gappei-johkasou by fish and pcr-dgge. | fluorescent in situ hybridization (fish) method with 16s rrna-targeted oligonucleotide probes was used for quantitative estimation of ammonia oxidizing bacteria (aob) and nitrite oxidizing bacteria (nob) in a johkasou. although the occupation ratios of aob and nob increased as nitrification progressed, about one month later, the occupation ratios decreased, despite showing good nitrification ability. furthermore, even when urea was added to the feeding wastewater to raise the amount of t-n, the ... | 2002 | 12523740 |
| [study of bacteriology on local application of tinidazole stilus in treatment for periodontitis]. | the study was designed to evaluate the antiseptic efficacy of tinidazole stilus against periodontal pathogen. | 2001 | 12539636 |
| [sugar phosphorylation activities in acetogenic bacteria]. | seven acetogenic bacteria (acetitomaculum ruminis, acetobacterium woodii, eubacterium limosum as well as isolates a2, a4, a10 and h3hh) were tested for pep- and atp-dependent phosphorylation of glucose and 2-deoxyglucose. although all organisms had detectable phosphorylation activity, substantial variation existed in the rates of both pep- and atp-dependent phosphorylation. isolate alo had the highest rate of pep-dependent phosphorylation of 11.62 nmol.l-1.mg-1.min-1. isolate a10, h3hh as well a ... | 1999 | 12555560 |
| molecular and biochemical characterization of two tungsten- and selenium-containing formate dehydrogenases from eubacterium acidaminophilum that are associated with components of an iron-only hydrogenase. | two gene clusters encoding similar formate dehydrogenases (fdh) were identified in eubacterium acidaminophilum. each cluster is composed of one gene coding for a catalytic subunit ( fdha-i, fdha-ii) and one for an electron-transferring subunit ( fdhb-i, fdhb-ii). both fdha genes contain a tga codon for selenocysteine incorporation and the encoded proteins harbor five putative iron-sulfur clusters in their n-terminal region. both fdhb subunits resemble the n-terminal region of fdha on the amino a ... | 2003 | 12560990 |
| the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector. | the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ... | 2003 | 12562362 |
| modification of intestinal flora in the treatment of inflammatory bowel disease. | because the intestinal microflora play an important role in the development of inflammatory bowel disease (ibd), there is currently some interest in the manipulation of the composition of the microflora towards a potentially more remedial community. this review summarizes the clinical and experimental efficacy of the manipulation of microflora by the use of prebiotics, probiotics, synbiotics, and antibiotics in ibd. prebiotics, defined as nondigestible food ingredients that beneficially affect t ... | 2003 | 12570821 |
| effects of alternative dietary substrates on competition between human colonic bacteria in an anaerobic fermentor system. | duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. a panel of group-specific fluorescent in situ hybridization probes targeting 16s rrna sequences revealed that the fermentors supported growth of a greater proportion of bacteroides and a lower proportion of gram-positive anaerobes related to faecalibacterium prausnitzii, ruminococcus flavefaciens-ruminococcus bromii, eubacterium rectale-cl ... | 2003 | 12571040 |
| phylogenetic characterization and molecular evolution of bacterial endosymbionts in psyllids (hemiptera: sternorrhyncha). | most sternorrhynchan insects harbor endosymbiotic bacteria in specialized cells (bacteriocytes) near the gut which provide essential nutrients for hosts. in lineages investigated so far with molecular methods (aphids, mealybugs, whiteflies), endosymbionts apparently have arisen from independent infections of common host ancestors and co-speciated with their hosts. some endosymbionts also exhibit putatively negative genetic effects from their symbiotic association. in this study, the identity of ... | 1998 | 12572614 |
| treatment of ulcerative colitis by feeding with germinated barley foodstuff: first report of a multicenter open control trial. | germinated barley foodstuff (gbf) is a prebiotic foodstuff that effectively increases luminal butyrate production by stimulating the growth of protective bacteria. in the first pilot study, gbf has been shown to reduce both clinical activity and mucosal inflammation in ulcerative colitis (uc). the aim of this study was to investigate the efficacy of gbf in the treatment of uc in a multicenter open control trial. | 2002 | 12572869 |
| diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients. | the primary purpose of the present study was to compare the microbial profiles of the tongue dorsa of healthy subjects and subjects with halitosis by using culture-independent molecular methods. our overall goal was to determine the bacterial diversity on the surface of the tongue dorsum as part of our ongoing efforts to identify all cultivable and not-yet-cultivated species of the oral cavity. tongue dorsum scrapings were analyzed from healthy subjects with no complaints of halitosis and subjec ... | 2003 | 12574246 |
| active lipoprotein precursors in the gram-positive eubacterium lactococcus lactis. | lipid-modified proteins play important roles at the interface between eubacterial cells and their environment. the importance of lipoprotein processing by signal peptidase ii (spase ii) is underscored by the fact that this enzyme is essential for viability of the gram-negative eubacterium escherichia coli. in contrast, spase ii is not essential for growth and viability of the gram-positive eubacterium bacillus subtilis. this could be due to alternative amino-terminal lipoprotein processing, whic ... | 2003 | 12584195 |
| complementary impact of paralogous oxa1-like proteins of bacillus subtilis on post-translocational stages in protein secretion. | in mitochondria, chloroplasts, and gram-negative eubacteria, oxa1p(-like) proteins are critical for the biogenesis of membrane proteins. here we show that the gram-positive eubacterium bacillus subtilis contains two functional oxa1p orthologues, denoted spoiiij and yqjg. the presence of either spoiiij or yqjg is required for cell viability. whereas spoiiij is required for sporulation, yqjg is dispensable for this developmental process. the stability of two membrane proteins was found to be mildl ... | 2003 | 12586834 |
| eubacterium bacteremia and colon cancer. | eubacterium bacteremia is rare. we report a senile patient who presented with 3 episodes of bacteremia, caused by clostridium perfringens, eubacterium limosum and escherichia coli, respectively, which led to the diagnosis of adenocarcinoma of the colon. a differential diagnosis of colon cancer should be considered in patients with eubacterium bacteremia. | 2002 | 12587635 |
| possible quorum sensing in the rumen microbial community: detection of quorum-sensing signal molecules from rumen bacteria. | the bioluminescence assay using vibrio harveyi bb170 was used to examine quorum-sensing autoinducer 2 (ai-2) activity from cell-free culture fluids of rumen bacteria. the assay showed that the culture fluids of four species of rumen bacteria, butyrivibrio fibrisolvens, eubacterium ruminantium, ruminococcus flavefaciens, and succinimonas amylolytica, contained ai-2-like molecules. furthermore, homologues for luxs genes were detected in rumen fluids collected from three cows and in bacterial cells ... | 2003 | 12594022 |
| structure and function of an archaeal homolog of survival protein e (surealpha): an acid phosphatase with purine nucleotide specificity. | the survival protein e (sure) family was discovered by its correlation to stationary phase survival of escherichia coli and various repair proteins involved in sustaining this and other stress-response phenotypes. in order to better understand this ancient and well-conserved protein family, we have determined the 2.0a resolution crystal structure of surealpha from the hyperthermophilic crenarchaeon pyrobaculum aerophilum (pae). this first structure of an archaeal sure reveals significant similar ... | 2003 | 12595266 |
| characterization of a cellobiose phosphorylase from a hyperthermophilic eubacterium, thermotoga maritima msb8. | the cepa putative gene encoding a cellobiose phosphorylase of thermotoga maritima msb8 was cloned, expressed in escherichia coli bl21-codonplus-ril and characterized in detail. the maximal enzyme activity was observed at ph 6.2 and 80 degrees c. the energy of activation was 74 kj/mol. the enzyme was stable for 30 min at 70 degrees c in the ph range of 6-8. the enzyme phosphorolyzed cellobiose in an random-ordered bi bi mechanism with the random binding of cellobiose and phosphate followed by the ... | 2002 | 12596851 |
| the tungsten-containing formate dehydrogenase from methylobacterium extorquens am1: purification and properties. | nad-dependent formate dehydrogenase (fdh1) was isolated from the alpha-proteobacterium methylobacterium extorquens am1 under oxic conditions. the enzyme was found to be a heterodimer of two subunits (alpha1beta1) of 107 and 61 kda, respectively. the purified enzyme contained per mol enzyme approximately 5 mol nonheme iron and acid-labile sulfur, 0.6 mol noncovalently bound fmn, and approximately 1.8 mol tungsten. the genes encoding the two subunits of fdh1 were identified on the m. extorquens am ... | 2003 | 12605683 |
| isolation, characterization and electron microscopic single particle analysis of the nadh:ubiquinone oxidoreductase (complex i) from the hyperthermophilic eubacterium aquifex aeolicus. | the proton-translocating nadh:ubiquinone oxidoreductase (complex i) has been purified from aquifex aeolicus, a hyperthermophilic eubacterium of known genome sequence. the purified detergent solubilized enzyme is highly active above 50 degrees c. the specific activity for electron transfer from nadh to decylubiquinone is 29 u/mg at 80 degrees c. the a. aeolicus complex i is completely sensitive to rotenone and 2-n-decyl-quinazoline-4-yl-amine. sds polyacrylamide gel electrophoresis shows that it ... | 2003 | 12627969 |
| bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. | one of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus bacillus, is described on the basis of phenotypic characterization, 16s rdna sequence analysis and dna-dna hybridization studies. it is a gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. the spores of this novel bacterial species exhibited resistance to uv, gamma-radiation, h2o2 and desiccation. the 18s rdna sequence analysis revealed a clear affiliation between t ... | 2003 | 12656168 |
| butyrivibrio hungatei sp. nov. and pseudobutyrivibrio xylanivorans sp. nov., butyrate-producing bacteria from the rumen. | two novel gram-negative, anaerobic, non-spore-forming, butyrate-producing bacterial species, strains mz 5t and jk 615t, were isolated from the rumen fluid of cow and sheep. both strains were curved rods that were motile by means of single polar or subpolar flagellum and common in the rumen microbial ecosystem. strain mz 5t produced high xylanase, proteinase, pectin hydrolase and dnase activities; 1,4-beta-endoglucanase was also detected in the culture medium. the bacterium utilized a wide range ... | 2003 | 12656174 |
| clostridium colicanis sp. nov., from canine faeces. | morphological, biochemical and molecular genetic studies were performed on an unknown, anaerobic, rod-shaped organism isolated from faeces of a canine. the organism was tentatively identified as a member of the genus clostridium based on its cellular morphology and ability to form endospores but, biochemically, it did not appear to correspond to any recognized species of this genus. comparative 16s rrna gene sequence analysis showed that the bacterium represents a previously unrecognized subline ... | 2003 | 12656182 |
| high-resolution x-ray structure of the dna-binding protein hu from the hyper-thermophilic thermotoga maritima and the determinants of its thermostability. | the histone-like dna-binding proteins (hu) are a convenient model for studying factors affecting thermostability because of their relatively simple, easily comparable structures, their common function, and their presence in organisms of widely differing thermostability. we report the determination of the high-resolution structure (1.53 a) at 273 k and 100 k of the hu protein from the hyper-thermophilic eubacterium thermotoga maritima(hu tmar, t(m)=80.5 degrees c). the structural data presented c ... | 2003 | 12664263 |
| an immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions. | we immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. the bacteria in the specimens were detected by brown-brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. seventy percent of the examined teeth showed bacteria in ... | 2003 | 12669880 |
| phylogenetic analysis of anaerobic psychrophilic enrichment cultures obtained from a greenland glacier ice core. | the examination of microorganisms in glacial ice cores allows the phylogenetic relationships of organisms frozen for thousands of years to be compared with those of current isolates. we developed a method for aseptically sampling a sediment-containing portion of a greenland ice core that had remained at -9 degrees c for over 100,000 years. epifluorescence microscopy and flow cytometry results showed that the ice sample contained over 6 x 10(7) cells/ml. anaerobic enrichment cultures inoculated w ... | 2003 | 12676695 |
| the structure of the antigenic polysaccharide produced by eubactrium saburreum t15. | the antigenic polysaccharide was obtained from the cell wall of eubacterium saburreum strain t15 by trypsin digestion followed by gel permeation and ion-exchange chromatography. its structure was determined using acid hydrolysis, methylation analysis, and 1d and 2d nmr spectroscopy. it contained l-threo-pent-2-ulose (xul), d-fucose (fuc), and d-glycero-d-galacto-heptose (hep) in 2:3:3 ratio. methylation analysis indicated an octasaccharide repeating-unit containing five branches. the 1h and 13c ... | 2003 | 12681915 |
| detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam. | a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ... | 2003 | 12682151 |
| mitochondrial connection to the origin of the eukaryotic cell. | phylogenetic evidence is presented that primitively amitochondriate eukaryotes containing the nucleus, cytoskeleton, and endomembrane system may have never existed. instead, the primary host for the mitochondrial progenitor may have been a chimeric prokaryote, created by fusion between an archaebacterium and a eubacterium, in which eubacterial energy metabolism (glycolysis and fermentation) was retained. a rickettsia-like intracellular symbiont, suggested to be the last common ancestor of the fa ... | 2003 | 12694174 |
| aquifex aeolicus trna (gm18) methyltransferase has unique substrate specificity. trna recognition mechanism of the enzyme. | transfer rna (guanosine-2')-methyltransferase (gm-methylase) catalyzes the transfer of a methyl group from s-adenosyl-l-methionine to 2'-oh of g18 in the d-loop of trna. based on their mode of trna recognition, gm-methylases can be divided into the following two types: type i having broad specificity toward the substrate trna, and type ii that methylates only limited trna species. protein synthesized by in vitro cell-free translation revealed that gm-methylase encoded in the aquifex aeolicus gen ... | 2003 | 12704200 |
| new bacterial species associated with chronic periodontitis. | recent investigations of the human subgingival oral flora based on ribosomal 16s cloning and sequencing have shown many of the bacterial species present to be novel species or phylotypes. the purpose of the present investigation was to identify potential periodontal pathogens among these newly identified species and phylotypes. species-specific ribosomal 16s primers for pcr amplification were developed for detection of new species. associations with chronic periodontitis were observed for severa ... | 2003 | 12709498 |
| identification and nucleotide sequence analysis of a cryptic plasmid, prm21, from rhodothermus marinus. | here we report the identification and nucleotide sequence analysis of prm21, a plasmid isolated from the thermophilic eubacterium rhodothermus marinus. prm21 consists of 2935 bp, has a g+c content of 58.2% and one major open reading frame whose deduced product shows significant similarities to repa proteins from several plasmids, the highest being to the repa of psa from escherichia coli. a region with the characteristics of iteron-containing replicons, three 19 bp repeats, dnaa boxes, an a+t ri ... | 2003 | 12726773 |
| eubacterium callanderi bacteremia: report of the first case. | eubacterium callanderi is an environmental anaerobic rod-shaped bacterium first isolated in 1998 from an industrial anaerobic digester. we report on the first clinical isolate of e. callanderi, which was recovered from the blood of a patient with a bladder carcinoma. identification of the organism was made by cell fatty acid chromatographic analysis and 16s rrna gene sequencing. | 2003 | 12734289 |
| transformation of flavonoids by intestinal microorganisms. | fruit, vegetables and cereals contain a wealth of secondary plan metabolites which have been implicated in the promotion of health. to understand the mechanism of their action it is necessary to gain more information on their fate in the body following ingestion. a certain proportion of ingested secondary plant constituents may escape absorption in the small intestine and therefore undergo transformation by intestinal microorganisms or enterohepatic circulation. to study the transformation of se ... | 2003 | 12747214 |
| degradation of arginine and other amino acids by butyrate-producing asaccharolytic anaerobic gram-positive rods in periodontal pockets. | the use of 20 amino acids by butyrate-producing asaccharolytic anaerobic gram-positive rods (aagprs) in periodontal pockets, i.e. eubacterium minutum, filifactor alocis, e. infirmum, e. sulci and e. saphenum, was studied. e. minutum used only arginine and lysine, and produced substantial amounts of butyrate and ammonia as the main metabolic products from arginine, and acetate, butyrate and ammonia from lysine. fi. alocis used arginine alone and produced butyrate and ammonia. e. infirmum, e. sulc ... | 2003 | 12749914 |
| in vitro activities of dalbavancin and nine comparator agents against anaerobic gram-positive species and corynebacteria. | dalbavancin is a novel semisynthetic glycopeptide with enhanced activity against gram-positive species. its comparative in vitro activities and those of nine comparator agents, including daptomycin, vancomycin, linezolid, and quinupristin-dalfopristin, against 290 recent gram-positive clinical isolates strains, as determined by the nccls agar dilution method, were studied. the mics of dalbavancin at which 90% of various isolates tested were inhibited were as follows: actinomyces spp., 0.5 microg ... | 2003 | 12760876 |
| interconversion of the product specificity of type i eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution. | prenyltransferases catalyze the sequential condensation of isopentenyl diphosphate into prenyl diphosphates with specific chain lengths. pioneering studies demonstrated that the product specificities of type i prenyltransferases were mainly determined by the amino acid residues at the 4th and 5th positions before the first aspartate-rich motif (farm) of the prenyltransferases. we previously cloned a type i geranylgeranyl diphosphate synthase (ggdpsase) gene from streptomyces griseolosporeus mf73 ... | 2003 | 12761202 |
| molecular diversity of mesophilic and thermophilic bacteria in a membrane bioreactor determined by fluorescent in situ hybridization with mxaf- and rrna-targeted probes. | an evaluation of the efficiency of treatment of kraft mill foul condensates in a membrane bioreactor was carried out in the laboratory. efficiency and rate of methanol removal were quantified at operating temperatures of 35, 45 and 55 degrees c. the structure of the bacterial community present in the reactor biomass at the different operating temperatures was evaluated by in situ hybridization of the biomass samples with fluorescently-labelled probes (fish) targeting the eubacteria, the alpha, b ... | 2003 | 12761771 |
| proteomic studies of diauxic lag in the differentiating prokaryote streptomyces coelicolor reveal a regulatory network of stress-induced proteins and central metabolic enzymes. | bacteria typically undergo intermittent periods of starvation and adaptation, emulated as diauxic growth in the laboratory. in association with growth arrest elicited by metabolic stress, the differentiating eubacterium streptomyces coelicolor not only adapts its primary metabolism, but can also activate developmental programmes leading to morphogenesis and antibiotic biosynthesis. here, we report combined proteomic and metabolomic data of s. coelicolor used to analyse global changes in gene exp ... | 2003 | 12787356 |
| a lupus-like syndrome develops in mice lacking the ro 60-kda protein, a major lupus autoantigen. | antibodies against a conserved rna-binding protein, the ro 60-kda autoantigen, occur in 24-60% of all patients with systemic lupus erythematosus. anti-ro antibodies are correlated with photosensitivity and cutaneous lesions in these patients and with neonatal lupus, a syndrome in which mothers with anti-ro antibodies give birth to children with complete congenital heart block and photosensitive skin lesions. in higher eukaryotes, the ro protein binds small rnas of unknown function known as y rna ... | 2003 | 12788971 |
| pcr methodology as a valuable tool for identification of endodontic pathogens. | this paper reviews the principles of polymerase chain reaction (pcr) methodology, its application in identification of endodontic pathogens and the perspectives regarding the knowledge to be reached with the use of this highly sensitive, specific and accurate methodology as a microbial identification test. | 2003 | 12799118 |
| molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. | chronic maxillary sinusitis is a chronic inflammatory condition in which the role of microbial infection remains undefined. bacteria have been isolated from chronically inflamed sinuses; however, their role in the chronicity of inflammation is unknown. the objective of this study was to determine whether bacteria are present in clinical samples from chronic maxillary sinusitis and to assess the diversity of the flora present. washes and/or tissue samples from endoscopic sinus surgery on 11 patie ... | 2003 | 12808082 |
| in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, bacitracin, and four other antimicrobials against intestinal anaerobic bacteria. | by using an agar dilution method, the in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, and five other agents were determined against 300 gram-positive and 54 gram-negative strains of intestinal anaerobes. ramoplanin was active at <or=2 microg/ml against 287 of 300 (95.7%) gram-positive organisms, including 18 strains of clostridium difficile for which mics of ramoplanin were 0.25 to 0.5 microg/ml; for 3 of these, linezolid mics were 8 to 16 micro g/ml. nineteen clostridium ... | 2003 | 12821492 |
| molecular characterization of the presence of eubacterium spp and streptococcus spp in endodontic infections. | eubacterium spp. and streptococcus spp. are virulent, commonly identified microorganisms in endodontic infections. the purpose of this study was to use molecular methods to identify these organisms in 22 infected root canals that include eight cases with preoperative clinical symptoms and five cases with a history of diabetes mellitus. the presence of streptococcus spp. and eubacterium spp. was examined using two sets of pcr primers specific with multiple species within the respective genera. po ... | 2003 | 12823801 |
| the extracellular proteome of bacillus subtilis under secretion stress conditions. | the accumulation of malfolded proteins in the cell envelope of the gram-positive eubacterium bacillus subtilis was previously shown to provoke a so-called secretion stress response. in the present studies, proteomic approaches were employed to identify changes in the extracellular proteome of b. subtilis in response to secretion stress. the data shows that, irrespective of the way in which secretion stress is imposed on the cells, the levels of only two extracellular proteins, htra and yqxi, dis ... | 2003 | 12823817 |
| structural basis for thermostability of beta-glycosidase from the thermophilic eubacterium thermus nonproteolyticus hg102. | the three-dimensional structure of a thermostable beta-glycosidase (gly(tn)) from the thermophilic eubacterium thermus nonproteolyticus hg102 was determined at a resolution of 2.4 a. the core of the structure adopts the (betaalpha)(8) barrel fold. the sequence alignments and the positions of the two glu residues in the active center indicate that gly(tn) belongs to the glycosyl hydrolases of retaining family 1. we have analyzed the structural features of gly(tn) related to the thermostability an ... | 2003 | 12837801 |
| oligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria in human feces. | 16s rrna-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. three new cluster-specific probes detected bacteria related to roseburia intestinalis, faecalibacterium prausnitzii, and eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study ... | 2003 | 12839823 |
| treatment of portal vein septic thrombosis by infusion of antibiotics and an antifungal agent into portal vein and superior mesenteric artery: a case report. | a 44-year-old man was hospitalized because of right-sided abdominal pain and fever. on admission, he presented spike fever, jaundice, and renal failure. abdominal ultrasonography and computed tomography showed extensive thrombus in the portal vein. anaerobic bacteria were identified in arterial and portal blood cultures. based on these findings, septic thrombosis of the portal vein was diagnosed. intraportal infusion of antibiotics, urokinase and an antifungal agent, followed by infusion of anti ... | 2003 | 12845997 |
| temperature-dependent presteady state kinetics of lumazine synthase from the hyperthermophilic eubacterium aquifex aeolicus. | 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase) catalyzes the condensation of 5-amino-6-ribitylamino-2,4(1h,3h)-pyrimidinedione and 3,4-dihydroxy-2-butanone 4-phosphate. presteady state kinetic experiments using the enzyme from the hyperthermophilic bacterium aquifex aeolicus were monitored by multiwavelength photometry. an early optical transient absorbing around 330 nm is interpreted as a schiff base intermediate obtained by reaction of the position 5 amino group of the heterocycli ... | 2003 | 12867427 |
| effect of garenoxacin on eubacteria in the normal intestinal microflora when administered concomitantly with digoxin. | garenoxacin is a new des-f(6)-quinolone with a broad antimicrobial spectrum. it has been reported that antibiotics may raise digoxin concentrations in the plasma of patients who are taking these agents concurrently, possibly due to the effect on the digoxin-metabolizing intestinal microflora. sixteen healthy subjects were given a loading dose of digoxin (0.25 mg orally, q 6 h) on day 1 followed by once-daily doses of 0.25 mg on days 2 through 14. the subjects also received garenoxacin 600 mg ora ... | 2003 | 12868550 |
| a large-scale comparison of genomic sequences: one promising approach. | we introduce a novel, linguistic-like method of genome analysis. we propose a natural approach to characterizing genomic sequences based on occurrences of fixed length words from a predefined, sufficiently large set of words (strings over the alphabet [a, c, g, t]). a measure based on this approach is called compositional spectrum and is actually a histogram of imperfect word occurrences. our results assert that the compositional spectrum is an overall characteristic of a long sequence i.e., a c ... | 2003 | 12870770 |
| eubacterium pyruvativorans sp. nov., a novel non-saccharolytic anaerobe from the rumen that ferments pyruvate and amino acids, forms caproate and utilizes acetate and propionate. | two similar gram-positive rods were isolated from 10(-6) dilutions of ruminal fluid from a sheep receiving a mixed grass hay/concentrate diet, using a medium containing pancreatic casein hydrolysate as sole source of carbon and energy. the isolates did not ferment sugars, but grew on pyruvate or trypticase, forming caproate as the main fermentation product and valerate to a lesser extent. acetate and propionate were utilized. one of these strains, i-6t, was selected for further study. strain i-6 ... | 2003 | 12892112 |
| highly thermostable amylase and pullulanase of the extreme thermophilic eubacterium rhodothermus marinus: production and partial characterization. | five strains of the extreme thermophilic rhodothermus marinus were screened for the production of amylolytic and pullulytic activities. the culture medium for the selected strain, r. marinus iti 990, was optimized using central composite designs for enhanced enzyme production. the optimized medium containing 1.5 gl(-1) of maltose and 8.3 gl(-1) of yeast extract yielded amylase, pullulanase and alpha-glucosidase activities of 45, 33 and 2.1 nkatml(-1), respectively. among the various carbon sourc ... | 2003 | 12895565 |
| identification of aquifex aeolicus trna (m2(2g26) methyltransferase gene. | the modifications of n2,n2-dimethylguanine (m2(2)g) are found in trnas and rrnas from eukarya and archaea. in trnas, modification at position g26 is generated by trna (m2(2)g26) methyltransferase, which is encoded by the corresponding gene, trm1. this enzyme catalyzes the methyl-transfer from s-adenosyl-l-methionine to the semi-conserved residue, g26, via the intermediate modified base, m2g26. recent genome sequencing project has been reported that the putative trm1 is encoded in the genome of a ... | 2002 | 12903189 |
| coaggregation of streptococcus salivarius with periodontopathogens: evidence for involvement of fimbriae in the interaction with prevotella intermedia. | streptococcus salivarius is divided into two serological subgroups that carry either fibrils or fimbriae. although fimbriae have been observed on up to 50% of s. salivarius strains in the human oral cavity, no function has yet been assigned to them. to determine whether s. salivarius fimbriae have a role in adhesion, we examined the ability of s. salivarius to coaggregate with selected microorganisms involved in periodontal diseases. our results show that s. salivarius coaggregated with fusobact ... | 2003 | 12930529 |
| composition of microbiota in content and mucus from cecae of broiler chickens as measured by fluorescent in situ hybridization with group-specific, 16s rrna-targeted oligonucleotide probes. | six group-specific 16s rrna-targeted oligonucleotide probes were used to investigate the composition of the microbiota of cecal content and mucus from broiler chickens. together, the probes hybridized to as many as 94.7% of the bacteria detectable with the universal probe bact338 in the content of the cecum of 2-d-old chicks. fewer bacteria gave signals with these probes as the birds aged, and coverage was as low as 76% for the bacteria in cecal content of a 6-wk-old chicken. in the cecal conten ... | 2003 | 12943294 |
| characterization of microbial communities and composition in constructed dairy wetland wastewater effluent. | constructed wetlands have been recognized as a removal treatment option for high concentrations of contaminants in agricultural waste before land application. the goal of this study was to characterize microbial composition in two constructed wetlands designed to remove contaminants from dairy washwater. water samples were collected weekly for 11 months from two wetlands to determine the efficiency of the treatment system in removal of chemical contaminants and total and fecal coliforms. the red ... | 2003 | 12957887 |
| reduced sulfhydryls maintain specific incision of bpde-dna adducts by recombinant thermoresistant bacillus caldotenax uvrabc endonuclease. | prokaryotic dna repair nucleases are useful reagents for detecting dna lesions. escherichia coli uvrabc endonuclease can incise dna containing uv photoproducts and bulky chemical adducts. the limited stability of the e. coli uvrabc subunits leads to difficulty in estimating incision efficiency and quantitative adduct detection. to develop a more stable enzyme with greater utility for the detection of dna adducts, thermoresistant uvrabc endonuclease was cloned from the eubacterium bacillus caldot ... | 2003 | 12963345 |
| probable identity of the growth promoting factor for butyribacterium rettgeri with other biologically-active substances. | 1952 | 12999673 | |
| [study of a new strictly anaerobic species of the genus eubacterium: eubacterium crispatum n. sp..]. | 1953 | 13124957 | |
| effects of dietary fibre-rich juice colloids from apple pomace extraction juices on intestinal fermentation products and microbiota in rats. | effects of colloids isolated from apple pomace extraction juices (so-called b-juices) produced by enzymic liquefaction on food intake, body and faecal weights, short-chain fatty acid (scfa) profile and selected intestinal microbiota were investigated in rats. ten male wistar rats per group were fed diets without any apple dietary fibre (df) (control) or supplement with 5 % b-juice colloids or an alcohol-insoluble substance (ais) from apples for 6 weeks. rats fed with apple df (5 % b-juice colloi ... | 2003 | 13129467 |
| tracer experiments on the mechanism of acetate formation from carbon dioxide by butyribacterium rettgeri. | 1954 | 13183932 | |
| metabolism of glucose by butyribacterium rettgeri. | 1954 | 13183933 | |
| susceptibility of proteus and providence bacilli to 10 antibacterial agents. | 1961 | 13693187 | |
| [carbamyl phosphate, intermediate in the degradation of creatinine by enzymatic extracts of eubacterium sarcosinogenum]. | 1960 | 13774693 | |
| [isolation of a polynucleotide phosphorylase from a strictly anaerobic bacteria]. | 1961 | 13776892 | |
| [various biological aspects of epileptic convulsive crises]. | 1959 | 13793708 | |
| [the behavior of c-reactive protein in premature infants]. | 1959 | 13794114 | |
| [fermentation of pyroracemic acid by cultures of butyric bacteria]. | 1960 | 13795410 | |
| [c-reactive protein in pregnancy, labor and puerperium]. | 1960 | 13795524 | |
| [c-reactive protein in the cerebrospinal fluid and blood in various disease states]. | 1960 | 13796553 | |
| evaluation of tests for c-reactive protein and anti-streptolysin 'o'. | 1960 | 13797330 | |
| [the c-reactive protein reaction in infantile pathology. iii. behavior within the framework of the humoral modifications of various diseases]. | 1959 | 13797582 | |
| [the c-reactive protein in the cerebrospinal fluid in the course of bacterial meningitis]. | 1960 | 13800204 | |
| [cytologic study of the action of sea water on some anaerobic bacteria]. | 1960 | 13800727 | |
| [on the determination of c-reactive protein in blood]. | 1959 | 13801123 | |
| [the "c-reactive protein" in syphilis]. | 1960 | 13813693 | |
| [c-reactive protein as a detector of inflammatory processes in blood donors]. | 1959 | 13814963 | |
| [acute phase protein and acute cardiac death]. | 1959 | 13817785 | |
| [the behavior of c-reactive protein in echincococcosis]. | 1959 | 13822527 | |
| serologic and fractionation studies of c-reactive protein. | 1960 | 13823177 | |
| [the behavior of the c-reactive protein in some primary and metastatic tumors]. | 1960 | 13824342 | |
| a method for quantitative determination of c-reactive protein using gel-diffusion. | 1959 | 13825593 | |
| [antistreptolysin, antistrepto-hyaluronidase and c-reactive protein levels in rheumatic disease in children]. | 1959 | 13826263 | |
| [c-reactive protein and gamma x-globulin in bantus of the lower congo]. | 1960 | 13832830 | |
| [detection of the c-reactive protein in the cerebrospinal fluid by the anderson-mccarty method]. | 1960 | 13833850 | |
| [c-reactive protein in acute coronary disease and its diagnostic and prognostic role]. | 1960 | 13834805 |