Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| saliva protein binding to layers of oral streptococci in vitro and in vivo. | this paper reports a system for measuring saliva protein binding to oral streptococci. enamel chips with layers of streptococcus gordonii blackburn or streptococcus oralis 10557 were incubated in vitro with whole saliva from eight persons. blackburn bound significantly more amylase than 10557; no strain differences were seen for lysozyme or lactoferrin. there were significant correlations between saliva and bound amylase and lactoferrin. blackburn and 10557 chips were then placed in ten subjects ... | 1995 | 7543122 |
| effect of lactobacillus rhamnosus strain gg (atcc 53103) on the growth of streptococcus sobrinus in vitro. | lactobacillus gg, a recently characterized l. rhamnosus gg strain (atcc 53103), has been shown to exert inhibitory activity against a variety of bacterial species, including streptococci. we isolated and studied the effect of the inhibitory substance of lactobacillus gg on some oral streptococci. the inhibitory activity of the isolated substance was weak, but some growth inhibition was observed in streptococcus sobrinus pretreated with the substance in comparison with untreated controls. zones o ... | 1995 | 7552958 |
| salivary amylase promotes adhesion of oral streptococci to hydroxyapatite. | recent studies have demonstrated that several species of oral streptococci, such as streptococcus gordonii, bind soluble salivary alpha-amylase. the goal of the present study was to determine if amylase immobilized onto a surface such as hydroxyapatite can serve as an adhesion receptor for s. gordonii. initially, human parotid saliva was fractionated on bio-gel p60, and fractions were screened for their ability to promote adhesion of s. gordonii to hydroxyapatite. fractions containing alpha-amyl ... | 1995 | 7560386 |
| sucrose-dependent accumulation of oral streptococci and their adhesion-defective mutants on saliva-coated hydroxyapatite. | the adhesion and accumulation of oral streptococci on saliva-coated hydroxyapatite was examined in strains representing species that appear in initial plaque (streptococcus sanguise fc1 and streptococcus oralis c5) and in more mature plaque (streptococcus gordonii g9b). washed cells of strains fc1 and c5 did not attach better to saliva-coated hydroxyapatite than did strain g9b, suggesting that the degree of initial adhesiveness does not alone account for the temporal appearance of these bacteria ... | 1995 | 7567067 |
| corrosion of intra-oral magnets in the presence and absence of biofilms of streptococcus sanguis. | intra-oral magnets are used in dentistry for a variety of purposes, and their susceptibility to corrosion is of great clinical importance. although a number of in vitro models have been developed to study corrosion of intra-oral magnets, none have attempted to determine the possible contribution to corrosion made by oral bacteria, which are known to form a biofilm on intra-oral appliances. we have exposed demagnetized neodymium/iron/boron (nd2fe14b) magnets to an artificial saliva in the presenc ... | 1995 | 7578777 |
| treatment with various antibiotics of experimental endocarditis caused by penicillin-resistant streptococcus sanguis. | streptococcus sanguis currently accounts for one-half of viridans streptococci. treatment has become complicated due to the increase in resistance to penicillin and cephalosporins. the aim of the study was to assess the efficacy of various antibiotics as monotherapy and in association with gentamicin, in a experimental model of infective endocarditis in rabbits. the effects were compared with a control group and a group given classical penicillin-gentamicin treatment. | 1995 | 7588902 |
| oral streptococci with genetic determinants similar to the glucosyltransferase regulatory gene, rgg. | the streptococcus gordonii challis glucosyltransferase structural gene, gtfg, is positively regulated by the upstream gene, rgg, the only described gtf regulatory determinant in oral streptococci. southern hybridization analyses indicated that rgg-like and gtfg-like determinants were present on the same hindiii fragment in strains of s. gordonii, streptococcus sanguis, and streptococcus oralis, whereas no rgg-like determinants were detected in mutans streptococci, streptococcus mitis, and strept ... | 1995 | 7591096 |
| [re-replacement of starr-edwards mitral valve (model 6320) for prosthetic valve endocarditis 22 years after replacement]. | the patient was a 43-year-old woman who had undergone mitral valve replacement with starr-edwards ball valve (model 6320) 22 years ago. perivalvular mitral leakage due to prosthetic valve endocarditis and tricuspid regurgitation were found. she underwent re-replacement of the mitral valve. the operative findings revealed almost intact valvular function of the ball valve. as far as we know, 22 years is the longest follow-up for the starr-edwards ball valve which has maintained normal function. | 1995 | 7594857 |
| adenocarcinoma of the colon presenting as streptococcus sanguis bacteremia. | 1995 | 7639245 | |
| bacteremia in conjunction with endodontic therapy. | this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ... | 1995 | 7641631 |
| characterization of inhibitory effects of suspected periodontopathogens on osteogenesis in vitro. | by using an in vitro bone-forming culture system, the chick periosteal osteogenesis (cpo) model, the direct effects on osteogenesis of sonicated extracts derived from oral bacteria were examined. both extracts from bacterial species having strong associations with periodontal diseases (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and prevotella intermedia, hereinafter referred to as suspected periodontopathogens) and extracts from species not correlated with periodontal diseas ... | 1995 | 7642257 |
| platelet receptors for the streptococcus sanguis adhesin and aggregation-associated antigens are distinguished by anti-idiotypical monoclonal antibodies. | platelets aggregate in response to an adhesin and the platelet aggregation-associated protein (paap) expressed on the cell surfaces of certain strains of streptococcus sanguis. we sought to identify the corresponding paap receptor and accessory adhesin binding sites on platelets. since the adhesion(s) of s. sanguis for platelets has not been characterized, an anti-idiotype (anti-id) murine monoclonal antibody (mab2) strategy was developed. first, mab1s that distinguished the adhesin and paap ant ... | 1995 | 7642300 |
| development of a flow method for susceptibility testing of oral biofilms in vitro. | bacteria in biofilms are known to be more resistant than bacteria in batch cultures to antimicrobial agents. the purpose of the present study was to develop a flow method for formation of oral biofilms permitting susceptibility testing of plaque bacteria. a brain heart infusion (bhi) streptococcus sanguis 804 culture was pumped through a modified robbins device (mrd) with 25 exchangeable silicone disks at 40 ml/h. after 24-48 h disks were removed and biofilm cells dispersed by vortex mixing and ... | 1995 | 7654358 |
| competitive binding of calcium and magnesium to streptococcal lipoteichoic acid. | equilibrium dialysis was used to investigate the binding capacity and affinity of lipoteichoic acid (lta) from the gram-positive bacterium streptococcus sanguis for calcium and magnesium by a competitive method. lta was shown to bind approx. 1 mol of either calcium or magnesium per mole phosphate. calcium and magnesium dissociation constants were found to be 8.39 +/- 0.31 mmol/l and 15.01 +/- 2.01 mmol/l respectively, indicating that s. sanguis lta will preferentially bind calcium. lta may act a ... | 1995 | 7654772 |
| carcinoma of the colon presenting as streptococcus sanguis bacteremia. | bacteremia by streptococci that normally inhabit the gastrointestinal tract has been associated with colon carcinoma. such association is best known for streptococcus bovis, but has also been reported for other streptococci. in the present communication a patient is described who presented with streptococcus sanguis bacteremia and was subsequently found to suffer from an adenocarcinoma of the sigmoid. a possible association between bacteremia by commensal streptococci of both the upper and lower ... | 1995 | 7661185 |
| immunoglobulin a-metallo-type specific prolyl endopeptidases. | 1995 | 7674950 | |
| delmopinol interactions with cell walls of gram-negative and gram-positive oral bacteria. | the main purpose of the present study was to investigate the influence of delmopinol hydrochloride on the cell surface morphology of gram-negative and gram-positive bacterial cells by using transmission electron microscopy. a second purpose was to evaluate the extraction of cell wall material caused by delmopinol and the binding of radiolabelled delmopinol to the various strains. fresh isolates and type strains of gram-negative rods associated with periodontal disease, porphyromonas gingivalis, ... | 1995 | 7675514 |
| species identification of oral viridans streptococci by restriction fragment polymorphism analysis of rrna genes. | oral streptococci formerly classified as streptococcus sanguis have been divided into six genetic groups. methods to identify those species by genotype are needed. this study compared restriction fragment polymorphisms of rrna genes (ribotypes) for seven s. gordonii, three s. sanguis, four s. oralis, three s. mitis, one s. crista, and seven s. parasanguis strains classified in previous dna hybridization studies, as well as one clinical isolate. dna was digested with hindiii, pvuii, hindiii and p ... | 1993 | 7691875 |
| demonstration of antigen-specific immune response against streptococcus sanguis. | the genetic control of streptococcus sanguis antigen response was studied. mice sensitized with inactivated s. sanguis organisms antigen-injected at the base of the tail developed footpad swelling. those with an i-ak,q,r region of h-2 showed a strong footpad response, whereas those with an i-ab,d,s region showed a weak response to s. sanguis cell wall antigen. footpad response was mediated by cd4+,8- t cells by using in vitro monoclonal antibody treatment. similar evidence of genetic control was ... | 1993 | 7694648 |
| heterologous expression of an endoglucanase gene (enda) from the ruminal anaerobe ruminococcus flavefaciens 17 in streptococcus bovis and streptococcus sanguis. | the heterologous expression of a cloned endoglucanase gene (enda) from the ruminal bacterium ruminococcus flavefaciens 17 was demonstrated in the streptococcus species s. bovis jb1 and s. sanguis dl1. the enda gene was introduced into s. bovis and s. sanguis using the escherichia coli/streptococcus shuttle vector pva838. expression of the gene was detected by clearing zones around the recombinant colonies on agar plates containing carboxymethylcellulose stained with congo red. s. bovis jb1 conta ... | 1995 | 7705607 |
| postantibiotic effects and postantibiotic sub-mic effects of benzylpenicillin on viridans streptococci isolated from patients with infective endocarditis. | we investigated the postantibiotic effects (paes) and the postantibiotic sub-mic effects of benzylpenicillin on three strains of viridans streptococci isolated from infective endocarditis patients. the paes of benzylpenicillin on penicillin tolerant streptococcus sanguis tw-70 (0.4-3.9 h), penicillin tolerant s. sanguis tw-80 (0.3-6.3 h) and nontolerant streptococcus oralis tw-186 (0.5-3.1 h) were dependent on exposure time. the paes were not concentration dependent for s. sanguis tw-70 and s. s ... | 1994 | 7706164 |
| cryopreserved homografts in aortic and mitral prosthetic endocarditis: expanding the use of biological tissues in complex cardiac infections. | the case of a 27-year-old male heroin addict suffering from mitral and aortic prosthetic valve endocarditis is presented. double valve re-replacement was performed using cryopreserved aortic homografts. aortic root replacement with coronary re-implantation and intra-atrial valve implantation for mitral valve replacement were the techniques used. despite the fatal outcome of this case, it clearly illustrates the possibilities of expanding the indications for combined complex replacement of heart ... | 1993 | 7719510 |
| streptococci and actinomyces inhibit regrowth of streptococcus mutans on gnotobiotic rat molar teeth after chlorhexidine varnish treatment. | clinical studies suggest that the long-term suppression of mutans streptococci on tooth surfaces after intensive chlorhexidine therapy is mainly due to bacterial interference. other streptococci and also actinomyces naeslundii are proposed to inhibit regrowth of mutans streptococci after suppression by the agent. we have tested this hypothesis in gnotobiotic rats associated with streptococcus mutans alone, or associated with s. mutans and strains of streptococcus oralis, streptococcus sanguis, s ... | 1995 | 7728832 |
| adherence of candida albicans to a cell surface polysaccharide receptor on streptococcus gordonii. | candida albicans atcc 10261 and ca2 bound to cells of the oral bacteria streptococcus gordonii, streptococcus oralis, and streptococcus sanguis when these bacteria were immobilized onto microtiter plate wells, but they did not bind to cells of streptococcus mutans or streptococcus salivarius. cell wall polysaccharide was extracted with alkali from s. gordonii nctc 7869, the streptococcal species to which c. albicans bound with highest affinity, and was effective in blocking the coaggregation of ... | 1995 | 7729891 |
| bacterial-stimulated cytokine production of peripheral mononuclear cells from patients of various periodontitis categories. | periodontitis is a general term for disease categories, including juvenile periodontitis (jp), rapidly progressive periodontitis (rpp), and adult periodontitis (ap), which may or may not share a common etiology and pathogenesis. these disease categories are characterized by differences in progression of tissue destruction and differences in age group susceptibility, but not, to our knowledge, by differences in cytokine responses of inflammatory cells. the present study examined blood cell counts ... | 1995 | 7730965 |
| identification of pioneer viridans streptococci in the oral cavity of human neonates. | three hundred and sixty-seven strains of pioneer streptococci isolated from the mouths of 40 healthy, full-term infants during the first month of life were examined by two taxonomic schemes that incorporated biochemical and physiological characteristics, iga1 protease production and glycosidase activities. streptococcus mitis biovar 1 and s. oralis comprised 55.0% of the pioneer streptococci isolated from neonates. s. salivarius constituted 25.3% of the isolates, while s. anginosus, s. mitis bio ... | 1995 | 7739028 |
| post-antibiotic effect of azithromycin and erythromycin on streptococcal susceptibility to phagocytosis. | the effect of azithromycin and erythromycin on growth, cell surface hydrophobicity and the susceptibility to the bactericidal activity of human polymorphonuclear leucocytes (pmnl) was examined in four streptococcus species. exposure to either 10 x mic azithromycin or erythromycin induced a post-antibiotic effect (pae) of between 2.4 and 4.3 h. erythromycin caused a longer pae for s. sanguis than azithromycin under the same conditions. the cell surface charge (hydrophobic or hydrophilic) of the s ... | 1995 | 7752216 |
| a 23 kda membrane glycoprotein bearing neunac alpha 2-3gal beta 1-3galnac o-linked carbohydrate chains acts as a receptor for streptococcus sanguis omz 9 on human buccal epithelial cells. | streptococcus sanguis colonizes several human oral surfaces, including both hard and soft tissues. large salivary mucin-like glycoproteins bearing sialic acid residues are known to bind various s.sanguis strains. however, the molecular basis for the adhesion of s.sanguis to human buccal epithelial cells (hbec) has not been established. the present study shows that s.sanguis omz 9 binds to exfoliated hbec in a sialic acid-sensitive manner. the desialylation of such cells invariably abolishes adhe ... | 1995 | 7772872 |
| bacterial adhesion to dental amalgam and three resin composites. | the ability of three oral bacteria to adhere to hydrophobic amalgam (water contact angle 60 degrees) and hydrophobic resin composites (prisma-ap.h 56 degrees. herculite xrv 82 degrees and z100 89 degrees) was compared using an in vitro assay. | 1995 | 7782529 |
| degradative enzymes of oral streptococci. | members of the streptococcus sanguis group (ssg) and streptococcus milleri group (smg) were screened for their ability to produce glycosidase, arylamidase (peptidase), protease, dextranase and glycosyltransferase activities. species within each group produced unique patterns of activity. the most commonly produced glycosidases were beta-d-glucosidase, beta-d-galactosidase, n-acetyl-beta-d-glucosaminidase and n-acetyl-beta-d-galactosaminidase and the least commonly produced glycosidase activity w ... | 1995 | 7786231 |
| quantifying the strength of bacterial adhesive interactions with salivary glycoproteins. | we adapted an assay that has been used to estimate the strength of eukaryotic cell-cell and cell-extracellular matrix adhesive interactions (mcclay et al., 1981) to quantify the strength of bacterial (streptococci, fusobacteria, actinomyces) interactions with salivary receptors. bacteria are centrifuged onto human submandibular/sublingual or parotid-saliva-coated microtiter wells. plates are sealed with pressure-sensitive, double-sided tape which allows them to be inverted and centrifuged again. ... | 1995 | 7790599 |
| recurrent streptococcus sanguis meningitis in a patient with a ventriculoperitoneal shunt. | 1994 | 7803666 | |
| low surface tension calcium hydroxide solution is an effective antiseptic. | the antimicrobial effects of a saturated calcium hydroxide solution, and in combination with 10% and 20% detergent, were evaluated on streptococcus faecalis, streptococcus sanguis, streptococcus mutans, streptococcus salivarius, neisseria sp., diphtheroid, staphylococcus aureus, lactobacillus sp., staphylococcus epidermidis, bacillus subtilis and candida albicans. the saturated calcium hydroxide solution was effective against only four of the 11 microorganisms studied over a 60-min exposure time ... | 1994 | 7806414 |
| the interaction of positively charged phospholipid vesicles with bacteria. | 1994 | 7821586 | |
| a theoretical approach to polyhydroxy-mediated interactions between liposomes and bacterial biofilms. | 1994 | 7821587 | |
| involvement of bactericidal factors from thrombin-stimulated platelets in clearance of adherent viridans streptococci in experimental infective endocarditis. | platelets activated with thrombin release bactericidal factors. we studied the role of the susceptibility of viridans streptococci to these bactericidal factors in the development of infective endocarditis (ie). by using the experimental endocarditis rabbit model, the initial adherence and the development of ie were assessed for 10 viridans streptococcal strains differing in their susceptibilities to releasate (material released) from thrombin-activated platelets. six strains were susceptible an ... | 1995 | 7822036 |
| cloning of an enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci. | serum from a patient with enterococcus faecalis endocarditis was used to identify the gene efaa cloned in lambda zapii in escherichia coli. nucleotide sequence analysis revealed a 924-bp open reading frame encoding a protein with a predicted molecular weight of 34,768. the amino acid sequence of efaa shows 55 to 60% homology to a group of streptococcal proteins, fima from streptococcus parasanguis, ssab from streptococcus sanguis, scaa from streptococcus gordonii, and psaa from streptococcus pne ... | 1995 | 7822045 |
| the effect of acid and fluoride release on the antimicrobial properties of four glass ionomer cements. | 1994 | 7831144 | |
| bacterial interference in vitro. comparison between a quantitative kinetic and a cocultivation blood agar test method. | the aim of the present study was to compare two methods for estimation of bacterial growth interference between various bacteria using a bioscreen robot analyzer, allowing kinetic documentation, and a cocultivation test on blood agar plates. six laboratory strains with different virulence and growth requirements were used: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mitis, staphylococcus aureus, and staphylococcus epidermidis. the interf ... | 1994 | 7833000 |
| identification of antimicrobial peptides by using combinatorial libraries made up of unnatural amino acids. | the use of water-soluble synthetic peptide combinatorial libraries permits the systematic examination of tens to hundreds of millions of peptides in existing microdilution assays. in the present study, we prepared and determined the antistaphylococcal activities of two new synthetic peptide combinatorial libraries (one n-acetylated, the other not) composed of tetrapeptides having one position defined and the remaining three positions made up of mixtures of l-, d-, and unnatural amino acids (a to ... | 1994 | 7840558 |
| a microbiological study of recurrent dentinal caries. | a cross-sectional microbiological study of recurrent (secondary) dentinal caries was conducted. freshly extracted human teeth containing amalgam restorations judged to be clinically intact were scrubbed with chlorhexidine soap and soaked in povidone-iodine solution. the teeth were then split to remove the fillings and examined for dentinal caries. of 54 teeth examined, 22 (40%) were free of dentinal caries in the restored site. the remainder were assigned to arbitrary groups of questionable (15) ... | 1994 | 7850843 |
| microbiota of rapidly progressive periodontitis lesions in association with clinical parameters. | the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ... | 1994 | 7853132 |
| a comparison of the microbial flora in carious dentine of clinically detectable and undetectable occlusal lesions. | it is not known whether the aetiology of occlusal hidden caries lesions (hcl) is identical to that of small visible lesions (svl). previous studies of the microflora of hcl suggest that relatively few species can be isolated. the aim of the present study was to compare the bacterial composition of dentine from 10 hcl and 17 svl in a population of children aged 8-18 years. the following bacteria were identified: actinomyces spp., mutans streptococci, streptococcus sanguis, streptococcus oralis, s ... | 1995 | 7867050 |
| altered expression of the platelet aggregation-associated protein from streptococcus sanguis after growth in the presence of collagen. | certain strains of streptococcus sanguis adhere selectively to human platelets (adh+) and, in plasma, induce them to aggregate into in vitro thrombi (agg+). the induction of aggregation is mediated by the platelet aggregation-associated protein (paap) expressed on the cell surface of the streptococcus. in endocarditis, expression of paap may be regulated by association with host proteins on damaged heart valves. to begin to test this hypothesis, three strains of s. sanguis were each cultured in ... | 1995 | 7868231 |
| adsorption of salivary proteins to the surface of oral streptococcal cells. | oral tissues, especially tooth surfaces, are covered with a layer of salivary proteins. oral bacterial cells that adsorb to salivary components accumulated on the tooth surface are, as a rule, covered with the same components, especially proteins. thus, it is possible that the salivary proteins covering the bacterial cells are related to the adhesion of bacteria to oral tissues. the aim of this study was to clarify the mechanisms of adsorption of salivary proteins to the surface of streptococcus ... | 1994 | 7869131 |
| effect of binding of fibrinogen to each bacterium on coaggregation between porphyromonas gingivalis and streptococcus oralis. | fibrinogen inhibits the coaggregation between porphyromonas gingivalis and streptococcus oralis. in this study, we determined which bacterium interacts with fibrinogen in this inhibitory process. although preincubation of each bacterium with fibrinogen did not inhibit coaggregation, its activity was completely eliminated by the addition of protease inhibitors such as n-ethylmaleimide (nem), p-chloromercuriphenyl sulfonate and n alpha-p-tosyl-l-lysine chloromethyl ketone to the preincubation mixt ... | 1994 | 7870471 |
| kinetics of lactose-reversible coadhesion of actinomyces naeslundii wvu 398a and streptococcus oralis 34 on the surface of hexadecane droplets. | most investigations of mechanisms accounting for intergeneric coaggregation have emphasized stereospecific rather than nonspecific interactions. the purpose of this investigation was to determine the relative importance of lectin-carbohydrate and nonspecific hydrophobic and ionic interactions, using a model based on strains with one of the most well understood specific coaggregation mechanisms, the lactose-reversible coaggregation of actinomyces naeslundii and streptococcus oralis. the kinetics ... | 1994 | 7870472 |
| oral health and implanted joint prostheses. | 1995 | 7873312 | |
| the antimicrobial activity of prevention mouthrinse. | prevention mouthrinse was designed to serve as a vital supplement to normal oral hygiene procedures. to determine the antimicrobial potency of this mouthrinse, minimal inhibitory concentrations (mics), bactericidal kinetics, and short-term exposure studies were conducted. a spectrum of oral microorganisms was employed in this investigation: streptococcus sanguis, streptococcus anginosus, streptococcus mutans, actinomyces viscosus, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, ei ... | 1993 | 7880467 |
| antiseptic mouthrinse-induced microbial cell surface alterations. | this study determined the effect of an essential oil-containing mouthrinse, listerine antiseptic, on microbial cell surface morphology using scanning electron microscopy. twenty-four hour cultures of c. albicans, a. viscosus, s. sanguis, f. nucleatum, and a. actinomycetemcomitans were harvested and washed in buffer. triplicate samples were overlaid on poly-l-lysine coated coverslips, immersed in either listerine or buffer for 30 seconds, fixed in glutaraldehyde followed by post-fixing in 1% osmi ... | 1993 | 7880474 |
| involvement of porphyromonas gingivalis fimbriae in adherence to streptococcus gordonii. | adherence of porphyromonas gingivalis to early plaque bacteria, such as streptococcus gordonii, is considered an important colonization mechanism. the molecules that mediate this interspecies binding have not been determined. fimbriae were prepared from p. gingivalis 33277 by mild agitation, ammonium sulfate precipitation and deae-sepharose chromatography. in a nitrocellulose blot adherence assay, purified fimbriae inhibited s. gordonii g9b-p. gingivalis 33277 binding by up to 54%. in addition, ... | 1993 | 7903442 |
| cell surface protein receptors in oral streptococci. | streptococci have a vast repertoire of adherence properties which include binding to human tissue components, epithelial cells and to other bacterial cells. these interactions are determined by the expression of cell-surface receptors some of which are species-specific. in the oral streptococci, two families of surface protein receptors with highly conserved amino acid sequences have been identified. the antigen i/ii family of polypeptides are wall-associated high molecular mass proteins (158-16 ... | 1994 | 7926661 |
| [löffler endocarditis fibroplastica with simultaneous infectious endocarditis]. | the clinical and immunological 8-year follow-up of a 45-year old female with hypereosinophilic syndrome and cardiac involvement of löffler's fibroplastic endocarditis which was complicated by infective endocarditis during immunosuppressive treatment is presented. all 3 stages of the disease were documented by clinical and histologic data: stage 1 by biopsy proven eosinophilic myocarditis and arteritis, stage 2 with berlin blue positive parietal thrombosis, and the fibrotic features of stage 3 bo ... | 1994 | 7927122 |
| nucleotide sequence of the streptococcus gordonii pk488 coaggregation adhesin gene, scaa, and atp-binding cassette. | human oral viridans group streptococci that coaggregate with actinomyces naeslundii pk606 express surface proteins related to scaa, the coaggregation-mediating adhesin of streptococcus gordonii pk488 (r. n. andersen, n. ganeshkumar, and p. e. kolenbrander, infect. immun. 61:981-987, 1993). the nucleotide sequence of the 6,125-bp ecori insert of pra1, containing scaa, the gene encoding scaa, was determined. six open reading frames (orfs) were identified. the orientation of four orfs, two upstream ... | 1994 | 7927711 |
| treatment with imipenem of experimental endocarditis caused by penicillin-resistant streptococcus sanguis. | the efficacy of imipenem alone or in combination with gentamicin for the treatment of experimental endocarditis in rabbits caused by a penicillin resistant strain of streptococcus sanguis was compared to that of gentamicin and penicillin g. in vitro, both beta-lactams were bactericidal in action and the combination of gentamicin and imipenem was synergistic after 4 h whereas penicillin g and gentamicin showed synergy after 8 h. antibiotic treatment was started two days after initiating infection ... | 1994 | 7928813 |
| detection of streptococcus pneumoniae dna in blood cultures by pcr. | we have developed a pcr assay, with primers derived from the autolysin (lyt) gene, for the detection of streptococcus pneumoniae dna in blood cultures. the predicted fragment of 247 bp was detected in all strains of pneumococci, embracing 12 different serotypes that were tested. although dna extracted from four viridans streptococci spp. streptococcus oralis, streptococcus mitis, streptococcus sanguis, and streptococcus parasanguis) gave amplification products, these were quite different from th ... | 1994 | 7929764 |
| evidence for an ecto-atpase on the cell wall of streptococcus sanguis. | certain strains of viridans streptococci bind platelets, which release atp from dense granules and then aggregate. by hydrolyzing the released atp to the platelet agonist, adp, cell wall-associated atpase activity of streptococcus sanguis may amplify the aggregation of platelets. to identify and characterize this ecto-atpase activity, whole cells were incubated with [14c]-atp. the cell-free nucleotides were separated by thin-layer chromatography and quantified by liquid scintillation counting. w ... | 1994 | 7936725 |
| stimulation of monocyte tissue factor expression in an in vitro model of bacterial endocarditis. | the coagulation system plays a major role in the formation of the infected endocardial vegetation in bacterial endocarditis. since monocytes can express tissue factor (tf) on their surfaces, they are thought to be responsible for the extrinsic activation of the coagulation cascade during this disease. the present study used an in vitro model in which fibrin plates, isolated adherent monocytes, and streptococcus sanguis were used as an analog for endocardial vegetations. adherence to fibrin by it ... | 1994 | 7960151 |
| action of sodium fluoride on phagocytosis by systemic polymorphonuclear leucocytes. | alterations in phagocytosis appear to be important in the onset and development of periodontitis. we investigated new substances that may be of use in the treatment of periodontitis. in a preliminary study, we tested the effect of sodium fluoride on phagocytosis by circulating polymorphonuclear leucocytes (pmns) in 10 replicate assays using blood from six healthy subjects. sodium fluoride was tested at concentrations of 1.0 micron to 4.0 microns against streptococcus oralis, streptococcus mutans ... | 1994 | 7962905 |
| streptococcus sanguis bacteremia and colorectal cancer: a case report. | streptococcus sanguis, usually considered a nonpathogen of the oral cavity, was isolated from blood cultures from a patient who was subsequently found to have a cecal adenocarcinoma. further studies are needed to determine if streptococcus sanguis infections have diagnostic implications similar to those of streptococcus bovis. | 1995 | 7968799 |
| a quantitative study of calcium binding by isolated streptococcal cell walls and lipoteichoic acid: comparison with whole cells. | calcium-binding by surface components of oral bacteria may have important effects on remineralization/demineralization phenomena and plaque cohesion. additionally, some species export large quantities of lipoteichoic acid, possibly as a protective measure. measurement of calcium-binding can facilitate prediction of how this will effectively buffer plaque fluid calcium concentration and affect these processes. using equilibrium dialysis, we measured calcium-binding capacities and affinities at ph ... | 1994 | 7983261 |
| the targeting of phospholipid liposomes to bacteria. | phospholipid liposomes have been prepared from phospholipid mixtures including dipalmitoylphosphatidylcholine/phosphatidylinositol (dppc/pi) and dppc/dipalmitoylphosphatidylglycerol (dppc/dppg) mixtures and targeted to adsorbed biofilms of the skin-associated bacteria staphylococcus epidermidis and proteus vulgaris and the oral bacterium streptococcus sanguis. the effects of time, liposome concentration and density of bacteria in the biofilm have been studied in detail for staphylococcus epiderm ... | 1994 | 7986811 |
| a study of the subgingival microflora of hiv-seropositive and cd4 cell depleted children with hemophilia. | the cultivable subgingival bacterial flora from three hiv-seropositive and cd4 cell depleted children with hemophilia were examined in this study. the numbers of cd4 cells of the subjected children ranged from 4.9 to 16.3 per mm3 blood. streptococcus species, including, beta-hemolytic streptococcus identified as streptococcus mutans and streptococcus sanguis, were predominant in the subgingival plaque samples. actinomyces species were also frequently found. gram-negative rods other than capnocyt ... | 1994 | 7987969 |
| antibacterial effectiveness of dentin bonding systems. | this study examined the antibacterial activities of several commercially available glass ionomer cements, dentin bonding systems and luting agents by employing both agar plate diffusion (apd) and growth inhibition (gi) methods. amalgam and resin composites were also tested as control materials. in both methods (apd and gi), cylindrical specimens were used. four bacteria strains were tested: streptococcus mutans, s. salivarius, s. mitis and s. sanguis. these studies were performed using standardi ... | 1993 | 7988764 |
| effects of subinhibitory concentrations of chemical agents on hydrophobicity and in vitro adherence of streptococcus mutans and streptococcus sanguis. | the aim of this investigation was to determine whether sublethal concentrations of chlorhexidine (cx), hexetidine (hx), cetylpyridinium chloride (cc), sodium dodecyl sulfate (sds), sanguinarine (sg), sodium fluoride (naf), and ammonium fluoride (nh4f) could affect hydrophobicity and adhesion of streptococcus sanguis and streptococcus mutans to saliva-coated hydroxyapatite (s-ha). determination of the minimum inhibitory concentrations (mics) showed that both species were susceptible to all agents ... | 1994 | 8001055 |
| the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration. | obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ... | 1994 | 8004324 |
| in vitro attachment of streptococcus sanguis to the dentin of the root canal. | the adhesion of a strain of streptococcus sanguis (nctc 7863) to the root canal dentin of bovine incisors was evaluated. samples (104) were prepared and smear layer was created on the root canal surface with a tungsten carbide bur. the samples were divided into four groups, one of them serving as a control sample and the other three each receiving a different treatment: 6% citric acid for 2 min (group 3), 6% citric acid for 2 min + 6.25% sodium hypochlorite for 1 min (group 2), 6% citric acid fo ... | 1994 | 8006568 |
| a comparative study of the aggregation of human, rat and rabbit platelets by members of the streptococcus sanguis group. | aggregation of platelets by bacteria is a potential factor in the pathogenesis of infective endocarditis. twenty-five strains from the streptococcus sanguis group, including 15 recent isolates from cases of endocarditis, were compared for their ability to aggregate human and rat platelets over periods of 15 and 25 min, respectively. in each case, 76% of strains caused aggregation; the median time to onset of aggregation was longer for human platelets (12 min) than for rat platelets (1 min). stra ... | 1994 | 8006939 |
| bactericidal action of tachyplesin i against oral streptococci. | tachyplesin i, a polycationic antimicrobial peptide isolated from hemocytes of horseshoe crabs, kills bacteria by disrupting the membrane potential of the cytoplasmic membrane. the present study shows that, among 36 oral streptococcal strains, 12 of 21 streptococcus sanguis, 3 streptococcus mutans, 9 streptococcus salivarius and 3 streptococcus milleri strains were susceptible to tachyplesin i, whereas 9 s. sanguis strains were resistant. interestingly, these resistant strains include the clinic ... | 1994 | 8008433 |
| interleukin 8 in serum in granulocytopenic patients with infections. | serum levels of interleukin 8 (il-8) were examined in eight patients with acute myeloid leukaemia during 16 courses of chemotherapy. the patients experienced 14 episodes of fever which occurred in periods with granulocyte counts < 0.5 x 10(9)/l. febrile episodes were classified as bacteriologically defined infection (n = 6), clinically defined infection (n = 2), and unexplained fever (n = 6). il-8 was detected in 18/25 (72%), 2/3 (67%) and 3/7 (43%) of the serum samples in the respective groups. ... | 1994 | 8011545 |
| [treatment of experimental endocarditis caused by penicillin-resistant streptococcus viridans]. | 1994 | 8011732 | |
| a quantitative comparison of selected bacteria in human carious dentine by microscopic counts. | the levels of selected cariogenic and obligately anaerobic bacteria have been compared in carious dentine taken from fissures, smooth surfaces, and root surfaces. the numbers of infected dentinal tubules were determined by immunohistological staining using species-specific antisera against selected bacteria. selective localization was observed; mutans streptococci were the predominant bacteria in dentine from fissures and smooth surface coronal caries, but not from root surface caries. the propo ... | 1994 | 8033185 |
| endocarditis due to high level gentamicin resistant enterococcus faecium. | we describe the first reported case in the literature of bacterial endocarditis caused by enterococcus faecium that was highly resistant to gentamicin. the case is unusual in that it followed a successfully treated streptococcus sanguis endocarditis. the micro-organism was susceptible only to the glycopeptide antibiotics, vancomycin and teicoplanin and to agents such as pristinamycin and daptomycin which are not routinely available for treatment. it illustrates the therapeutic dilemma posed by s ... | 1994 | 8034999 |
| association of salivary bacterial aggregating activity with sulfomucin. | the requirements of human salivary mucins for aggregating potential towards the common cariogenic oral bacteria, s. mutans and s. sanguis, were investigated. agglutination inhibition assays demonstrated that the aggregating capacity towards bacteria resides in the acidic mucin fraction. the inhibitory activity of the acidic mucin decreased only 2-4-fold following removal of sialic acid, whereas the desulfation caused a complete loss of the inhibitory potential against both bacteria. furthermore, ... | 1994 | 8038721 |
| antigenic relationships among immunoglobulin a1 proteases from haemophilus, neisseria, and streptococcus species. | to investigate the antigenic variation and relationships of immunoglobulin a1 (iga1) proteases among different species and genera, we examined a comprehensive collection of serine type and metallo-type iga1 proteases and corresponding antisera in enzyme neutralization assays. sharing of neutralizing epitopes of metallo-type iga1 proteases from streptococcus pneumoniae, streptococcus sanguis, streptococcus mitis, and streptococcus oralis and of serine type iga1 proteases from haemophilus and path ... | 1994 | 8039886 |
| microorganisms on toothbrushes at day-care centers. | the microflora on 44 toothbrushes at 4 day-care centers in the city of göteborg have been investigated as a presumptive risk factor for transmission of microorganisms by children. non-supervised toothbrushing without the use of toothpaste was performed at the day-care centers twice a day. streptococci, predominantly s. salivarius, s. sanguis, and s. mitis, were the most frequently recorded group of microorganisms and generally constituted the greatest part of the flora (on average, 50%). beta-he ... | 1994 | 8048328 |
| treatment of experimental endocarditis caused by penicillin-resistant streptococcus sanguis with different doses of teicoplanin. | streptococcus viridans continues to be the most frequent causal agent of infective endocarditis. treatment has become more complicated due to the increase in resistance to penicillin and cephalosporins. in order to study the possible efficacy of teicoplanin at low and high doses, this antibiotic was investigated in rabbits as a monotherapy and in association with gentamicin. the effects were compared with a control group and a group given classical penicillin-gentamicin treatment. infective endo ... | 1994 | 8051983 |
| lipoteichoic acid from streptococcus sanguis is a natural glucosyl acceptor for glucosyltransferases. | the synthesis of methanol insoluble-[14c]labeled-polymers from [14c]sucrose by s. mutans glucosyltransferases was stimulated up to four-fold by the presence of highly purified, lipoteichoic acid (lta) from s. sanguis. gel filtration chromatography (sepharose 4b) and ion exchange chromatography (deae-bio-gel a) of the [14c]labeled-polymers formed in the presence of [3h]glycerol-labeled-lta showed that high molecular weight, negatively charged [14c]labeled-[3h]glycerol-labeled-lta complexes were b ... | 1994 | 8060321 |
| dextranase enhances antibiotic efficacy in experimental viridans streptococcal endocarditis. | in endocarditis, exopolysaccharide production by viridans streptococci has been associated with delayed antimicrobial efficacy in cardiac vegetations. we compared the efficacies of temafloxacin alone and in combination with dextranase, an enzyme capable of hydrolyzing 20 to 90% of the bacterial glycocalyx, in a rabbit model of endocarditis. in in vivo experiments, rabbits were infected intravenously with 10(8) streptococcus sanguis organisms and were treated 6 days later with temafloxacin (50 mg ... | 1994 | 8067775 |
| relation between mouth and haematogenous infection in total joint replacements. | to investigate the source of infections associated with orthopaedic prostheses. | 1994 | 8086903 |
| [infectious crystalline keratopathy occurring after penetrating keratoplasty]. | infectious crystalline keratopathy is a chronic corneal infection, characterized by interlamellar opacities that are shaped like crystal formations. in the majority of cases, streptococcus viridans is cultured. we report the case of a 74 year old man, presenting with corneal infection in a donor graft after penetrating keratoplasty. a streptococcus sanguis was isolated. the clinical response to antibiotics was poor, with a slow and inexerable progression, leading to possible corneal regrafting. | 1994 | 8089423 |
| extraction, partial purification and characterization of a bacteriocin (fragicilin) produced by a strain of bacteroides fragilis isolated from callithrix penicillata. | a strain of bacteroides fragilis, isolated from the marmoset callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). two active fractions (36 and 150 kda) were isolated by chromatography. the bacteriocin exhibited iso- and heteroantagonism. it remained stable between ph 3 and 10 and at 60 degrees c for 24 h. pronase, trypsin, proteinase k and type vii protease inactivated the bacteriocin, giving evidence of its protein nature. | 1994 | 8090990 |
| bacteremia due to viridans streptococci in neutropenic patients: a review. | viridans streptococci have long been considered, with the exception of the ability to cause endocarditis, as minor pathogenic agents. more recently, however, these bacteria have become a major concern in neutropenic patients undergoing a chemotherapeutic treatment. in this high-risk population, they can be responsible for up to 39% of bacteremia cases and are the most frequent cause of this type of infection. the most frequently isolated species in blood cultures are streptococcus mitis and stre ... | 1994 | 8092175 |
| an appraisal of the virulence factors associated with streptococcal endocarditis. | platelet aggregation is believed to be a virulence factor in infective endocarditis. other factors may be adhesion to components of thrombotic vegetations, particularly platelets, fibronectin and fibrinogen. two strains from the streptococcus sanguis group (ssg) were chosen for comparative study on the basis that one aggregated both human and rat platelets and the other lacked this capacity. both strains caused endocarditis in the rat model but the aggregating strain was found in higher numbers ... | 1994 | 8107058 |
| identification of viridans streptococci associated with bacteraemia in neutropenic cancer patients. | twenty-three viridans streptococcal isolates from pyrexial neutropenic patients with various malignant diseases were studied in a comprehensive identification scheme. fourteen isolates were identified as streptococcus oralis, five as s. mitis and two as s. salivarius but the remaining two could not be identified reliably. the virulence mechanisms associated with the ability of these species to survive and grow in vivo require further investigation but may involve the production of specific glyco ... | 1994 | 8114071 |
| dental infections as a risk factor for acute myocardial infarction. | the so-called classic risk factors of coronary heart disease (chd) do not explain all its clinical and epidemiological features. recent evidence suggests that certain infections, among them dental infections, are involved in the pathogenesis of chd. case-control studies have revealed an association between dental infections and acute myocardial infarction and chronic coronary heart disease. a large epidemiological survey revealed an association between missing teeth and chd and a recent 14-year ... | 1993 | 8131788 |
| short-term microbiological and clinical effects of subgingival irrigation with an antimicrobial mouthrinse. | fifty chronic adult periodontitis patients completed a 6-week controlled, double-blind, split mouth clinical study to determine the effects of subgingival irrigation with an antimicrobial mouthrinse on periodontal microflora, supragingival plaque, and gingivitis when used as an adjunct to normal oral hygiene. qualifying subjects had at least four sites, two on each side of the mouth, with probing depths between 4 and 6 mm, which bled on gentle probing. following baseline examinations, subjects r ... | 1994 | 8133413 |
| antibacterial properties of eight dental cements. | the antibacterial action of a light-cured glass polyalkenoate vitrebond and an adhesive resin luting agent panavia ex was investigated and compared with six previously tested dental cements. the bacterial cultures used were six strains of streptococcus mutans, six of streptococcus sanguis and six cultures of carious debris from carious lesions. a modification of the agar diffusion method was used to test the antibacterial action of freshly mixed cements. in addition the cements were allowed to s ... | 1993 | 8144245 |
| streptococcus sanguis osteomyelitis of the l2-3 lumbar vertebrae in seronegative rheumatoid arthritis. | 1994 | 8162652 | |
| novel automated microbial screening of platelet concentrates. | the recovery of bacteria and the speed of detection of microbial growth in platelet concentrates (pc) were studied, comparing a novel automated blood culture system (bact/alert) with two traditional methods. full-scale experiments were performed with a total of 33 units of pc (average content 320 x 10(9) platelets per unit) obtained from pooled buffy coats. six strains known as possible pc contaminants were tested: s. epidermidis, s. aureus, p. aeruginosa, b. cereus, e. aerogenes, and s. sanguis ... | 1994 | 8167004 |
| molecular characterization of a plasmid-borne (pgt633) erythromycin resistance determinant (ermgt) from lactobacillus reuteri 100-63. | lactobacillus reuteri 100-63 contains a 9.8 kb plasmid (pgt633) encoding resistance to the macrolide-lincosamide-streptogramin (mls) type b antibiotics. the restriction endonucleases acci, bamhi, bcli, ecori, ecorv, hhai, hindii, hpai, kpni, sali, and sspi were employed to establish a physical map of pgt633. next, genetic transfer experiments were conducted to establish the host range of pgt633. the results revealed that pgt633 replicated autonomously and encoded constitutive resistance to eryth ... | 1994 | 8171126 |
| inhibition of hydroxyapatite crystal growth by lipoteichoic acid. | lipoteichoic acids were purified from freshly isolated strains of streptococcus sanguis and lactobacillus casei, and from a laboratory strain of strep. rattus bht. their effects on the seeded growth of hydroxyapatite in 1 mm cacl2, 7.5 mm kh2po4, 50 mm hepes (ph 7.2) were determined. at lipoteichoic acid concentrations of 25 microm organic phosphate (po) and higher, the growth of hydroxyapatite appeared to be inhibited, at 100 microm po inhibition was complete for several hours. in contrast, low ... | 1994 | 8185499 |
| streptococcus sanguis bacteremia during ciprofloxacin therapy of a diabetic foot ulcer. | 1994 | 8192181 | |
| infective endocarditis in newborn infants with structurally normal hearts. | twelve infants with structurally normal hearts were demonstrated to have intracardiac vegetations on echocardiography and diagnosed as having infective endocarditis. all were found in the right side of the heart, most frequently at the junction of the superior vena cava and the right atrium and in no case involved the tricuspid or pulmonary valves. most were diagnosed in the course of investigation of repeatedly positive blood cultures, despite appropriate antibiotic therapy. coagulase-negative ... | 1994 | 8193471 |
| role of amoxicillin serum levels for successful prophylaxis of experimental endocarditis due to tolerant streptococci. | the importance of amoxicillin serum profiles for successful prophylaxis of experimental endocarditis in rats was assessed. animals with catheter-induced vegetations were challenged intravenously with large inocula of streptococcus sanguis and received one of the following amoxicillin dosages: single or multiple bolus injection of 40 mg/kg; 40 mg/kg administered as a continuous infusion over 12 h; or either 9 or 18 mg/kg administered over 12 or 24 h, respectively. the regimen producing a single t ... | 1994 | 8195626 |
| flow-cytometric identification and detection of porphyromonas gingivalis by a lps specific monoclonal antibody. | the purpose of this study was to identify porphyromonas gingivalis (p. gingivalis) by flow cytometry (fcm) using a monoclonal antibody (mab) omr-bg1e directed to p. gingivalis-specific lipopolysaccharide (lps). the p. gingivalis strains atcc 33277, 381, eso75, w50, and a7a1 were selected for the study. fusobacterium nucleatum (f. nucleatum), prevotella intermedia (p. intermedia), campylobacter rectus (c. rectus), streptococcus sanguis (s. sanguis) and actinobacillus actinomycetemcomitans (a. act ... | 1994 | 8195974 |
| hospital practices influence the pattern of infective endocarditis. | to identify factors contributing to infective endocarditis at a major teaching hospital. | 1994 | 8202007 |
| in vivo activities and penetration of the two components of the streptogramin rp 59500 in cardiac vegetations of experimental endocarditis. | we evaluated the in vivo activity and the diffusion of radiolabelled rp 57669 (rpi) and rp 54476 (rpii), the two components of the injectable streptogramin rp 59500, alone or in combination, in aortic vegetations from experimental endocarditis in rabbits. rpi and rpii demonstrated in vitro bacteriostatic and bactericidal synergy against a clinical strain of staphylococcus aureus resistant to methicillin and susceptible to erythromycin. in experimental staphylococcal endocarditis, rp 59500 was as ... | 1994 | 8203835 |
| [a case of hypertrophic obstructive cardiomyopathy (hocm) complicated by infective endocarditis (ie) and mitral regurgitation (mr)]. | the case of a 56-year-old man who underwent mitral valve replacement due to hocm complicated by ie and mr is reported. preoperative 2d echocardiographic assessment of the mitral valve revealed systole anterior motion (sam) and vegetation, and color flow doppler echocardiographic examination revealed severe mitral regurgitation. the left ventricular out-flow tract gradient decreased from 140 mmhg preoperatively to 60 mmhg postoperatively. mitral valve replacement should be considered in patients ... | 1994 | 8207888 |
| antibacterial activity of dentinal bonding agents. | the susceptibility of five bacterial species to seven dentinal bonding agents was examined in vitro. agar diffusion tests using filterpaper disks containing 10 microl each of conditioner, primer, or resin were performed on blood agar and mitis salivarius bacitracin agar. chlorhexidine (0.2%) was used as a positive control. after incubation, zones of inhibited bacterial growth were measured. of all the compounds tested, gluma cleanser and gluma etchant showed the strongest growth inhibition for a ... | 1993 | 8210322 |