Publications

degradation of (+)-catechin by acinetobacter calcoaceticus mtc 127.	acinetobacter calcoaceticus mtc 127 was able to grow on catechin and protocatechuic acid (pca) as sole carbon source. cells induced with catechin oxidized catechin and pca at rates higher than cells of uninduced cultures. two aromatic compounds, pca and phloroglucinol carboxylic acid (pgca) were isolated from culture filtrate of cells grown in catechin and characterized by infrared spectrometry and high performance thin-layer chromatography. moreover, a. calcoaceticus mtc 127 produced high level ...	2003	12787923
efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of hansenula polymorpha.	a high frequency of transformation and an equal gene dosage between transformants are generally required for activity-based selection of mutants from a library obtained by directed evolution. an efficient library construction method was developed by using in vivo recombination in hansenula polymorpha. various linear sets of vectors and insert fragments were transformed and analyzed to optimize the in vivo recombination system. a telomere-originated autonomously replicating sequence (ars) of h. p ...	2003	12902228
dual labeling of pseudomonas putida with fluorescent proteins for in situ monitoring of conjugal transfer of the tol plasmid.	we describe here a dual-labeling technique involving the green fluorescent protein (gfp) and the red fluorescent protein (dsred) for in situ monitoring of horizontal gene transfer via conjugation. a gfpmut3b-tagged derivative of narrow-host-range tol plasmid (pwwo) was delivered to pseudomonas putida kt2442, which was chromosomally labeled with dsred by transposon insertion via biparental mating. green and red fluorescent proteins were coexpressed in donor p. putida cells. cells expressing both ...	2003	12902279
rapid identification of bacteria from positive blood cultures by terminal restriction fragment length polymorphism profile analysis of the 16s rrna gene.	bacteremia results in significant morbidity and mortality, especially among patient populations that are immunocompromised. broad-spectrum antibiotics are administered to patients suspected to have bloodstream infections that are awaiting diagnosis that depends on blood culture analysis. significant delays in identification of pathogens can result, primarily due to the dependence on growth-based identification systems. to address these limitations, we took advantage of terminal restriction fragm ...	2003	12904391
cloning and characterization of the fur gene from moraxella bovis.	a homologue of the ferric uptake regulator gene (fur) was isolated from moraxella bovis by degenerate polymerase chain reaction and cloning. fur protein of m. bovis exhibited 72.1% amino acid identity with acinetobacter calcoaceticus fur. western blot analysis showed a decrease of fur expression in response to sufficient-iron conditions compared with deficient-iron conditions. an electrophoretic mobility-shift assay indicated that fur protein binds to dna fragments containing a putative fur-box ...	2003	12906101
screening of bifidobacterium strains isolated from human faeces for antagonistic activities against potentially bacterial pathogens.	as probiotic bacteria, strains belonging to the genus bifidobacterium colonise the gastro-intestinal tract of humans and animals at the time of birth, and they are found in young as well as in adult individuals in great numbers. moreover, they can interact with the development of enteric infections by the production of antimicrobial metabolites. in this work 281 strains of bifidobacteria were anaerobically isolated from human faecal samples, supplied by volunteers of different ages (youngs, adul ...	2003	12906392
characterization of hybrid toluate and benzoate dioxygenases.	toluate dioxygenase of pseudomonas putida mt-2 (tado(mt2)) and benzoate dioxygenase of acinetobacter calcoaceticus adp1 (bado(adp1)) catalyze the 1,2-dihydroxylation of different ranges of benzoates. the catalytic component of these enzymes is an oxygenase consisting of two subunits. to investigate the structural determinants of substrate specificity in these ring-hydroxylating dioxygenases, hybrid oxygenases consisting of the alpha subunit of one enzyme and the beta subunit of the other were pr ...	2003	12949084
growth of escherichia coli in model distribution system biofilms exposed to hypochlorous acid or monochloramine.	bacteria indigenous to water distribution systems were used to grow multispecies biofilms within continuous-flow slide chambers. six flow chambers were also inoculated with an escherichia coli isolate obtained from potable water. the effect of disinfectants on bacterial populations was determined after exposure of established biofilms to 1 ppm of hypochlorous acid (cloh) for 67 min or 4 ppm of monochloramine (nh(2)cl) for 155 min. to test the ability of bacterial populations to initiate biofilm ...	2003	12957935
semiquantitative detection by real-time pcr of aspergillus fumigatus in bronchoalveolar lavage fluids and tissue biopsy specimens from patients with invasive aspergillosis.	a real-time pcr method was developed and used to detect aspergillus fumigatus mitochondrial dna (mtdna) in bronchoalveolar lavage (bal) fluids and tissue biopsy specimens. the analytical sensitivity of the assay was one a. fumigatus conidium per reaction, and the assay was linear at least over 4 orders of magnitude above the detection limit. bal fluids from 66 immunocompromised patients at risk of invasive pulmonary aspergillosis (ipa) and 33 immunocompetent controls and tissue biopsy specimens ...	2003	12958261
surveillance of pediatric infections in a teaching hospital in mato grosso do sul, brazil.	nosocomial infections (ni) result in considerably high mortality and morbidity rates, especially among pediatric patients. considering current worldwide changes, information about the occurrence of pathogens and susceptibility tests are now seen as decisive for optimizing treatment. the purpose of this research was to determine the frequency of microorganisms, antimicrobial and genetic profiles, and risk factors associated with nosocomial infections in a teaching hospital in campo grande, mato g ...	2003	12959687
prevalence and in-vitro antimicrobial susceptibility patterns of acinetobacter strains isolated from patients in intensive care units.	fifty-six acinetobacter species strains (49 acinetobacter baumanii, 5 acinetobacter calcoaceticus, 2 acinetobacter iwoffii) were detected using both conventional methods and gas chromatography of bacterial fatty acids with the midi sherlock microbial identification system. the susceptibilities of these strains to 16 antimicrobial agents were investigated by the disc-diffusion method according to the national committee for clinical laboratory standards. the production of extended-spectrum beta-la ...	2003	12964502
[utilization in competition of sodium beta-hydroxybutyrate by neisseria winogradskyi].		1952	13017136
[degradation metabolism of 2-3 butanediol and of acetoin by microorganisms; considerations on neisseria winogradskyi. i. investigations on 2-3 butanediol dehydrogenase].		1953	13124973
[pathogenic or saprophytic bacteria transforming glucose to gluconic acid, bacterium anitratum, b5w, moraxella lwoffi var. glucidolytica, neisseria winogradskyi].		1954	13171653
identification of species of brucella using fourier transform infrared spectroscopy.	fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ...	2003	14500003
molecular characterization of the acute inflammatory response to infections with gram-negative versus gram-positive bacteria.	sepsis caused by gram-negative bacteria and that caused by gram-positive bacteria often manifest similar clinical features. we investigated plasma proinflammatory cytokine profiles in patients with sepsis due to gram-positive and gram-negative bacteria and studied the cytokine production and differential gene regulation of leukocytes stimulated ex vivo with escherichia coli lipopolysaccharide or heat-killed staphylococcus aureus. concentrations of tumor necrosis factor alpha, interleukin 1 recep ...	2003	14500502
real-time pcr assay targets the 23s-5s spacer for direct detection and differentiation of legionella spp. and legionella pneumophila.	a real-time pcr for the abi prism 7000 system targeting the 23s-5s spacer of legionella spp. was developed. simultaneous detection and differentiation of legionella spp. and legionella pneumophila within 90 min and without post-pcr melting-curve analysis was achieved using two taqman probes. in sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.	2003	14532229
temporal changes in the bacterioplankton of a northeast ohio (usa) river.	to examine temporal changes in a bacterial community, water samples were collected monthly for one year from five sites along a major use-reuse river, the cuyahoga river, in northeastern ohio (usa). fluorescent in situ hybridization (fish) was used to enumerate population sizes of two species of common bacteria, pseudomonas putida and acinetobacter calcoaceticus; fish was also performed with a domain bacteria specific probe. in addition, the total bacteria (based on dapi staining), colony formin ...	2002	14552350
determination of decimal reduction time (d value) of chemical agents used in hospitals for disinfection purposes.	prior to the selection of disinfectants for low, intermediate and high (sterilizing) levels, the decimal reduction time, d-value, for the most common and persistent bacteria identified at a health care facility should be determined.	2003	14563217
prediction of reduction potential changes in rubredoxin: a molecular mechanics approach.	predicting the effects of mutation on the reduction potential of proteins is crucial in understanding how reduction potentials are modulated by the protein environment. previously, we proposed that an alanine vs. a valine at residue 44 leads to a 50-mv difference in reduction potential found in homologous rubredoxins because of a shift in the polar backbone relative to the iron site due to the different side-chain sizes. here, the aim is to determine the effects of mutations to glycine, isoleuci ...	2003	14581187
purification and characterization of membrane-bound quinoprotein quinate dehydrogenase.	several bacterial strains carrying quinoprotein quinate dehydrogenase (qdh) were screened through acetic acid bacteria and other bacteria. strong enzyme activity was found in the membrane fraction of gluconobacter melanogenus ifo 3294, g. oxydans ifo 3292, g. oxydans ifo 3244, and some strains of acinetobacter calcoaceticus. interestingly, in the membrane fraction of a. calcoaceticus ac3, which is unable to produce pyrroloquinoline quinone (pqq), fairly large amounts of apo-qdh were formed, and ...	2003	14586098
high-resolution differentiation of cyanobacteria by using rrna-internal transcribed spacer denaturing gradient gel electrophoresis.	for many ecological studies of cyanobacteria, it is essential that closely related species or strains can be discriminated. since this is often not possible by using morphological features, cyanobacteria are frequently studied by using dna-based methods. a powerful method for analysis of the diversity and dynamics of microbial populations and for checking the purity and affiliation of cultivated strains is denaturing gradient gel electrophoresis (dgge). we realized high-resolution discrimination ...	2003	14602623
analysis of bacterial dna patterns--an approach for controlling biotechnological processes.	optimisation of biotechnological processes catalysed by microbial cells requires detailed information about operational limits of the single cells. their performance is correlated with distinct physiological states. we related these states to cell cycle events, which were found to proceed extremely diversely in different bacterial strains. characteristic dna patterns were found flow cytometrically, depending on the type of strain, substrates and growth conditions involved; this information can b ...	2003	14607431
new bacterial pathway for 4- and 5-chlorosalicylate degradation via 4-chlorocatechol and maleylacetate in pseudomonas sp. strain mt1.	pseudomonas sp. strain mt1 is capable of degrading 4- and 5-chlorosalicylates via 4-chlorocatechol, 3-chloromuconate, and maleylacetate by a novel pathway. 3-chloromuconate is transformed by muconate cycloisomerase of mt1 into protoanemonin, a dominant reaction product, as previously shown for other muconate cycloisomerases. however, kinetic data indicate that the muconate cycloisomerase of mt1 is specialized for 3-chloromuconate conversion and is not able to form cis-dienelactone. protoanemonin ...	2003	14617643
synthesis and biological activities of diflunisal hydrazide-hydrazones.	several diflunisal hydrazide-hydrazone derivatives namely 2',4'-difluoro-4-hydroxybiphenyl-3-carboxylic acid [(5-nitro-2-furyl/substitutedphenyl)methylene] hydrazide (3a-o) have been synthesised. methyl 2',4'-difluoro-4-hydroxybiphenyl-3-carboxylate (1) and 2',4'-difluoro-4-hydroxybiphenyl-3-carboxylic acid hydrazide (2) were also synthesised and used as intermediate compounds. all synthesised compounds were screened for their antimycobacterial activity against mycobacterium tuberculosis h37 rv, ...	2003	14642333
recent advances in petroleum microbiology.	recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. the physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. new molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystem ...	2003	14665675
exposure to bioaerosols in a municipal sewage treatment plant.	microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ...	2003	14677919
occurrence and characterization of mercury resistance in the hyperthermophilic archaeon sulfolobus solfataricus by use of gene disruption.	mercury resistance mediated by mercuric reductase (mera) is widespread among bacteria and operates under the control of merr. merr represents a unique class of transcription factors that exert both positive and negative regulation on gene expression. archaea and bacteria are prokaryotes, yet little is known about the biological role of mercury in archaea or whether a resistance mechanism occurs in these organisms. the archaeon sulfolobus solfataricus was sensitive to mercuric chloride, and low-l ...	2004	14702312
improved method for polynucleotide probe-based cell sorting, using dna-coated microplates.	we developed an improved method for cultivation-independent sorting of bacterial cells. the technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the cells. target cells are immobilized during a second hybridization step in microplates that are coated with dna that is complementary to the probe sequence. the method was applied suc ...	2004	14711680
comparison in a rat thigh abscess model of imipenem, meropenem and cefoperazone-sulbactam against acinetobacter baumannii strains in terms of bactericidal efficacy and resistance selection.	we compared imipenem, meropenem and cefoperazone-sulbactam against hospital originated a. baumannii strains in terms of bactericidal efficacy and selection of resistant mutants during treatment in a rat thigh abscess model.	2004	14713318
pqq glucose dehydrogenase with novel electron transfer ability.	pqq glucose dehydrogenase from acinetobacter calcoaceticus (gdh-b) is one of the most industrially attractive enzymes, as a sensor constituent for glucose sensing, because of its high catalytic activity and insensitivity to oxygen. we attempted to engineer gdh-b to enable electron transfer to the electrode in the absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase (qh-edh) from comamonas testosteroni, which is composed of a pqq- ...	2004	14741705
genetic environment and transcription of ampc in an acinetobacter baumannii clinical isolate.	an ampc gene was cloned from a clinical isolate of acinetobacter baumannii (strain ran). dna sequencing and primer extension studies showed that ampc is transcribed from a promoter contained within a putative insertion sequence element which has been found to abut several different genes in acinetobacter spp.	2004	14742218
endophthalmitis caused by acinetobacter calcoaceticus. a profile.	to report the clinical and microbiological profile of endophthalmitis caused by acinetobacter calcoaceticus.	2003	14750622
improved laboratory enrichment for enterohemorrhagic escherichia coli by exposure to extremely acidic conditions.	analysis of food samples for e. coli o157:h7 using the standard u.s. food and drug administration procedure is frequently complicated by overgrowth of nontarget microorganisms. a new procedure was developed for enrichment of enterohemorrhagic e. coli (ehec) which utilizes exposure to ph 2.00 for 2 h. this procedure yielded larger populations of ehec than the standard method by factors ranging from 2.7 to 7.7 and, when age-stressed cultures were used, by factors ranging from 2.7 to 11.5. cultures ...	2004	14766610
biased distribution of dna uptake sequences towards genome maintenance genes.	repeated sequence signatures are characteristic features of all genomic dna. we have made a rigorous search for repeat genomic sequences in the human pathogens neisseria meningitidis, neisseria gonorrhoeae and haemophilus influenzae and found that by far the most frequent 9-10mers residing within coding regions are the dna uptake sequences (dus) required for natural genetic transformation. more importantly, we found a significantly higher density of dus within genes involved in dna repair, recom ...	2004	14960717
the trna world.		2004	14970379
oral bacterial flora of dogs with and without rabies: a preliminary study in thailand.	the authors studied the bacterial flora of the dog oral cavity and of bite wounds, aerobic bacteria were isolated from mouth swabs of 16 normal and 5 rabid dogs as well as from infected dog-bite wounds from 18 patients. a total of 20 different microbial species were recovered from mouth swab cultures. the most frequently isolated organisms were klebsiella pneumoniae ssp pneumoniae, escherichia coli, staphylococcus aureus, citrobacter freundii, enterobacter cloacae, acinetobacter calcoaceticus, a ...	2003	14971525
rapid quantification of yersinia enterocolitica in pork samples by a novel sample preparation method, flotation, prior to real-time pcr.	the development of real-time pcr thermal cycles in the late 1990s has opened up the possibility of accurate quantification of microorganisms in clinical, environmental, and food samples. however, a lack of suitable sample preparation methods that allow rapid quantification of the nucleic acids, remove pcr inhibitors, and prevent false-positive results due to dna originating from dead cells has limited the use of quantitative pcr. we have used for the first time a new variant of density gradient ...	2004	15004051
computer-assisted surveillance for detecting clonal outbreaks of nosocomial infection.	whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. we developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital-wide basis. monthly total isolates of 25 clinically significant hospital pathogens were tallied from 1991 to 1998 to form a database for future comparison. two different algorithm ...	2004	15004070
characteristics of a new enantioselective thermostable dipeptidase from brevibacillus borstelensis bcs-1 and its application to synthesis of a d-amino-acid-containing dipeptide.	a new thermostable dipeptidase gene was cloned from the thermophile brevibacillus borstelensis bcs-1 by genetic complementation of the d-glu auxotroph escherichia coli wm335 on a plate containing d-ala-d-glu. nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid sequence with an m(r) of 35,000. the deduced amino acid sequence of the dipeptidase exhibited 52% similarity with the dipeptidase from listeria monocytogenes. the enzyme was purifi ...	2004	15006780
a dna adenine methyltransferase of escherichia coli that is cell cycle regulated and essential for viability.	dna sequence analysis revealed that the putative yhdj dna methyltransferase gene of escherichia coli is 55% identical to the nostoc sp. strain pcc7120 gene encoding dna methyltransferase avaiii, which methylates adenine in the recognition sequence, atgcat. the yhdj gene was cloned, and the enzyme was overexpressed and purified. methylation and restriction analysis showed that the dna methyltransferase methylates the first adenine in the sequence atgcat. this dna methylation was found to be regul ...	2004	15028690
growth and lipase production of a psychrotrophic acinetobacter calcoaceticus in an msg-containing medium.		2004	15057708
functional properties of borrelia burgdorferi reca.	functions of the borrelia burgdorferi reca protein were investigated in escherichia coli reca null mutants. complementation with b. burgdorferi reca increased survival of e. coli reca mutants by 3 orders of magnitude at a uv dose of 2,000 microj/cm(2). the viability at this uv dose was about 10% that provided by the homologous reca gene. expression of b. burgdorferi reca resulted in survival of e. coli at levels of mitomycin c that were lethal to noncomplemented hosts. b. burgdorferi reca was as ...	2004	15060028
crystal structure of 4-chlorocatechol 1,2-dioxygenase from the chlorophenol-utilizing gram-positive rhodococcus opacus 1cp.	the crystal structure of the 4-chlorocatechol 1,2-dioxygenase from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, a fe(iii) ion-containing enzyme involved in the aerobic biodegradation of chloroaromatic compounds, has been solved by multiple wavelength anomalous dispersion using the weak anomalous signal of the two catalytic irons (1 fe/257 amino acids) and refined at a 2.5 a resolution (r(free) 28.7%; r factor 21.4%). the analysis of the structure and its comparison with the ...	2004	15060064
occurrence and phylogenetic diversity of sphingomonas strains in soils contaminated with polycyclic aromatic hydrocarbons.	bacterial strains of the genus sphingomonas are often isolated from contaminated soils for their ability to use polycyclic aromatic hydrocarbons (pah) as the sole source of carbon and energy. the direct detection of sphingomonas strains in contaminated soils, either indigenous or inoculated, is, as such, of interest for bioremediation purposes. in this study, a culture-independent pcr-based detection method using specific primers targeting the sphingomonas 16s rrna gene combined with denaturing ...	2004	15066784
evolutionary stability of dna uptake signal sequences in the pasteurellaceae.	the dna-uptake signal sequence (uss) of the bacterium haemophilus influenzae is highly over-represented in its genome (1,471 copies of the core sequence aagtgcggt), and dna fragments containing uss are preferentially taken up by competent cells. because this bias favors uptake of conspecific dna, usss are often considered a kind of mate recognition system in bacteria, acting as species-specific barriers against uptake of unrelated dna. however, the h. influenzae uss is highly over-represented in ...	2004	15070749
dissemination of a clone of unusual phenotype of pandrug-resistant acinetobacter baumannii at a university hospital in taiwan.	from december 2002 to february 2003, 15 isolates of pandrug-resistant unidentified acinetobacter species were recovered from seven patients treated on different wards or intensive care units. both 16s-23s rrna intergenic spacer pcr-restriction fragment length polymorphism profiles and sequence analysis of these isolates identified them as acinetobacter baumannii. this pandrug-resistant a. baumannii strain with an unusual phenotype could persist in humans for long periods and was widely dissemina ...	2004	15071042
a modular minimal cell model: purine and pyrimidine transport and metabolism.	a more complete understanding of the relationship of cell physiology to genomic structure is desirable. because of the intrinsic complexity of biological organisms, only the simplest cells will allow complete definition of all components and their interactions. the theoretical and experimental construction of a minimal cell has been suggested as a tool to develop such an understanding. our ultimate goal is to convert a "coarse-grain" lumped parameter computer model of escherichia coli into a gen ...	2004	15090651
partial analysis of the genomes of two nontypeable haemophilus influenzae otitis media isolates.	in 1995, the institute for genomic research completed the genomic sequence of a rough derivative of haemophilus influenzae serotype d, strain kw20. this sequence, though extremely useful in understanding the basic biology of h. influenzae, has yet to provide significant insight into our understanding of disease caused by nontypeable h. influenzae (nthi), because serotype d strains are not generally pathogens. in contrast, nthi strains are frequently mucosal pathogens and are the primary pathogen ...	2004	15102813
susceptibility of the acinetobacter calcoaceticus-a. baumannii complex to imipenem, meropenem, sulbactam and colistin.	the acinetobacter calcoaceticus-acinetobacter baumannii complex includes some of the most clinically relevant species of the genus acinetobacter due to their capacity to cause epidemic nosocomial outbreaks as well as their increasing resistance to antibiotics. susceptibility of acinetobacter strains varies greatly depending on origin, thus highlighting the importance of local analyses of susceptibility profiles. two hundred twenty-one strains of the a. calcoaceticus-a. baumannii complex were ide ...	2004	15120728
antibodies to prion and acinetobacter peptide sequences in bovine spongiform encephalopathy.	an amino acid sequence homology has been identified between the bovine prion sequence (rpvdq) and the acinetobacter calcoaceticus enzyme, uridine-diphosphate-n-acetyl glucosamine-1-carboxy-vinyl-transferase which also contains (rpvdq). class-specific iga, igg and igm antibodies against synthetic peptides containing the structurally related sequences present in bovine prion and a. calcoaceticus were measured in 189 bovine spongiform encephalopathy (bse) positive cattle, 127 bse negative cattle an ...	2004	15127836
spread of novel aminoglycoside resistance gene aac(6')-iad among acinetobacter clinical isolates in japan.	a novel aminoglycoside resistance gene, aac(6')-iad, encoding aminoglycoside 6'-n-acetyltransferase, was identified in acinetobacter genospecies 3 strain a-51. the gene encoded a 144-amino-acid protein, which shared modest identity (up to 36.7%) with some of the aminoglycoside 6'-n-acetyltransferases. the results of high-pressure liquid chromatography assays confirmed that the protein is a functional aminoglycoside 6'-n-acetyltransferase. the enzyme conferred resistance to amikacin, tobramycin, ...	2004	15155202
microbial hydroxylation of indole to 7-hydroxyindole by acinetobacter calcoaceticus strain 4-1-5.	a screening study yielded acinetobacter calcoaceticus strain 4-1-5, which is capable of hydroxylating indole to 7-hydroxyindole. strain 4-1-5 grew on terephthalate as the sole source of carbon and energy and hydroxylated indole to 7-hydroxyindole by cometabolism of indole using terephthalate as cosubstrate. strain 4-1-5 produced 0.574 mm of 7-hydroxyindole at 2.38 mm indole in 24 h with the cell growth.	2004	15170130
regulation of hypercompetence in legionella pneumophila.	although many bacteria are known to be naturally competent for dna uptake, this ability varies dramatically between species and even within a single species, some isolates display high levels of competence while others seem to be completely nontransformable. surprisingly, many nontransformable bacterial strains appear to encode components necessary for dna uptake. we believe that many such strains are actually competent but that this ability has been overlooked because standard laboratory condit ...	2004	15175295
typing and characterization of carbapenem-resistant acinetobacter calcoaceticus-baumannii complex in a chinese hospital.	this study was designed to investigate the prevalence of carbapenem-resistant acinetobacter calcoaceticus-baumannii complex (acb complex) and to type carbapenemases. the relatedness of 45 isolates of carbapenem-resistant acb complex collected from a clinical setting was analysed by pfge. the carbapenemases produced by these isolates were typed by ief, a three-dimensional test, 2-mercaptopropanoic acid inhibition assay, pcr and dna cloning and sequencing. results showed that all 45 isolates were ...	2004	15184537
mammalian wax biosynthesis. ii. expression cloning of wax synthase cdnas encoding a member of the acyltransferase enzyme family.	wax monoesters are synthesized by the esterification of fatty alcohols and fatty acids. a mammalian enzyme that catalyzes this reaction has not been isolated. we used expression cloning to identify cdnas encoding a wax synthase in the mouse preputial gland. the wax synthase gene is located on the x chromosome and encodes a member of the acyltransferase family of enzymes that synthesize neutral lipids. expression of wax synthase in cultured cells led to the formation of wax monoesters from straig ...	2004	15220349
microscale and molecular assessment of impacts of nickel, nutrients, and oxygen level on structure and function of river biofilm communities.	studies were carried out to assess the influence of nutrients, dissolved oxygen (do) concentration, and nickel (ni) on river biofilm development, structure, function, and community composition. biofilms were cultivated in rotating annular reactors with river water at a do concentration of 0.5 or 7.5 mg liter(-1), with or without a combination of carbon, nitrogen, and phosphorus (cnp) and with or without ni at 0.5 mg liter(-1). the effects of ni were apparent in the elimination of cyanobacterial ...	2004	15240316
induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in mycobacterium tuberculosis as it goes into a dormancy-like state in culture.	mycobacterium tuberculosis enters the host by inhalation of an infectious aerosol and replicates in the alveolar macrophages until the host's immune defense causes bacteriostasis, which leads the pathogen to go into nonreplicative drug-resistant dormancy. the dormant pathogen can survive for decades till the host's immune system is weakened and active tuberculosis develops. even though fatty acids are thought to be the major energy source required for the persistence phase, the source of fatty a ...	2004	15262939
determination of enzyme mechanisms by molecular dynamics: studies on quinoproteins, methanol dehydrogenase, and soluble glucose dehydrogenase.	molecular dynamics (md) simulations have been carried out to study the enzymatic mechanisms of quinoproteins, methanol dehydrogenase (mdh), and soluble glucose dehydrogenase (sgdh). the mechanisms of reduction of the orthoquinone cofactor (pqq) of mdh and sgdh involve concerted base-catalyzed proton abstraction from the hydroxyl moiety of methanol or from the 1-hydroxyl of glucose, and hydride equivalent transfer from the substrate to the quinone carbonyl carbon c5 of pqq. the products of methan ...	2004	15273299
partial purification and characterization of a non-cyanobacterial cyanophycin synthetase from acinetobacter calcoaceticus strain adp1 with regard to substrate specificity, substrate affinity and binding to cyanophycin.	this study reports, for the first time, purification and biochemical characterization of a cyanophycin synthetase from a non-cyanobacterial strain. cyanophycin synthetase of acinetobacter calcoaceticus strain adp1 was purified 69-fold from recombinant escherichia coli by two chromatographic steps and one novel affinity step utilizing the mg(2+)-dependent binding of the enzyme to cyanophycin. unlike cyanobacterial cyanophycin synthetases characterized so far, the purified enzyme from a. calcoacet ...	2004	15289556
biochemical characterization of styab from pseudomonas sp. strain vlb120 as a two-component flavin-diffusible monooxygenase.	pseudomonas sp. vlb120 uses styrene as a sole source of carbon and energy. the first step in this metabolic pathway is catalyzed by an oxygenase (stya) and a nadh-flavin oxidoreductase (styb). both components have been isolated from wild-type pseudomonas strain vlb120 as well as from recombinant escherichia coli. stya from both sources is a dimer, with a subunit size of 47 kda, and catalyzes the enantioselective epoxidation of cc double bonds. styrene is exclusively converted to s-styrene oxide ...	2004	15292130
crystal structure of a baeyer-villiger monooxygenase.	flavin-containing baeyer-villiger monooxygenases employ nadph and molecular oxygen to catalyze the insertion of an oxygen atom into a carbon-carbon bond of a carbonylic substrate. these enzymes can potentially be exploited in a variety of biocatalytic applications given the wide use of baeyer-villiger reactions in synthetic organic chemistry. the catalytic activity of these enzymes involves the formation of two crucial intermediates: a flavin peroxide generated by the reaction of the reduced fla ...	2004	15328411
carb-9, a carbenicillinase encoded in the vcr region of vibrio cholerae non-o1, non-o139 belongs to a family of cassette-encoded beta-lactamases.	the gene bla(carb-9) was located in the vibrio cholerae super-integron, but in a different location relative to bla(carb-7). carb-9 (pi 5.2) conferred beta-lactam mics four to eight times lower than those conferred by carb-7, differing at ambler's positions v97i, l124f, and t228k. comparison of the genetic environments of all reported bla(carb) genes indicated that the carb enzymes constitute a family of cassette-encoded beta-lactamases.	2004	15388476
methylation of 23s rrna nucleotide g745 is a secondary function of the rlmai methyltransferase.	several groups of gram-negative bacteria possess an rlma(i) methyltransferase that methylates 23s rrna nucleotide g745 at the n1 position. inactivation of rlma(i) in acinetobacter calcoaceticus and escherichia coli reduces growth rates by at least 30%, supposedly due to ribosome malfunction. wild-type phenotypes are restored by introduction of plasmid-encoded rlma(i), but not by the orthologous gram-positive gene rlma(ii) that methylates the neighboring nucleotide g748. nucleotide g745 interacts ...	2004	15388872
relations and functions of dye-linked formaldehyde dehydrogenase from hyphomicrobium zavarzinii revealed by sequence determination and analysis.	faoa, the gene of the dye-linked nad(p)-independent quinone-containing formaldehyde dehydrogenase of methylamine-grown hyphomicrobium zavarzinii strain zv 580 was sequenced and analyzed together with an apparent promoter region and adjoining genes in a 7.2-kb fragment of hyphomicrobial dna. the formaldehyde dehydrogenase, identified as a periplasmic enzyme by its signal sequence, is distantly related to the soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase of acinetobacter calcoac ...	2004	15480576
diagnosis and assessment of trachoma.	trachoma is caused by chlamydia trachomatis. clinical grading with the who simplified system can be highly repeatable provided graders are adequately trained and standardized. at the community level, rapid assessments are useful for confirming the absence of trachoma but do not determine the magnitude of the problem in communities where trachoma is present. new rapid assessment protocols incorporating techniques for obtaining representative population samples (without census preparation) may giv ...	2004	15489358
antimicrobial susceptibility testing of acinetobacter spp. by nccls broth microdilution and disk diffusion methods.	although both broth microdilution (bmd) and disk diffusion (dd) are listed by nccls as acceptable methods for testing acinetobacter spp. for antimicrobial susceptibility, few studies have compared the results generated by the two methods. we tested 196 isolates of acinetobacter spp. from nine u.s. hospitals and from the centers for disease control culture collection by using bmd and dd and clinically appropriate antimicrobial agents. categorical results for amikacin, ciprofloxacin, gatifloxacin, ...	2004	15528702
wiring of pqq-dehydrogenases.	the performance of pyrroloquinoline quinone (pqq) dependent alcohol dehydrogenase (adh) and two types of pqq-glucose dehydrogenases in solution and when immobilized on the carbon paste electrodes modified with ferrocene derivatives is investigated. the immobilization of adh consisting of pqq and four hemes improves its stability up to 10 times. both pqq and heme moieties are involved in the electron transport from substrate to electrode. the ferrocene derivatives improve the electron transport 1 ...	2004	15556370
ventilator associated pneumonia: comparison between quantitative and qualitative cultures of tracheal aspirates.	deferred or inappropriate antibiotic treatment in ventilator-associated pneumonia (vap) is associated with increased mortality, and clinical and radiological criteria are frequently employed to establish an early diagnosis. culture results are used to confirm the clinical diagnosis and to adjust or sometimes withdraw antibiotic treatment. tracheal aspirates have been shown to be useful for these purposes. nonetheless, little is known about the usefulness of quantitative findings in tracheal secr ...	2004	15566587
synthesis of novel lipids in saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase.	the bifunctional wax ester synthase/acyl-coenzyme a:diacylglycerol acyltransferase (ws/dgat) is the key enzyme in storage lipid accumulation in the gram-negative bacterium acinetobacter calcoaceticus adp1, mediating wax ester, and to a lesser extent, triacylglycerol (tag) biosynthesis. saccharomyces cerevisiae accumulates tags and steryl esters as storage lipids. four genes encoding a dgat (dga1p), a phospholipid:diacylglycerol acyltransferase (lro1p) and two acyl-coenzyme a:sterol acyltransfera ...	2004	15574908
quinate oxidation in gluconobacter oxydans ifo3244: purification and characterization of quinoprotein quinate dehydrogenase.	quinoprotein quinate dehydrogenase (qdh) is a membrane-bound enzyme containing pyrroloquinoline quinone (pqq) as the prosthetic group. qdh in gluconobacter oxydans ifo3244 was found to be inducible by quinate and it is not constitutively expressed in the absence of quinate. the purification of holo-form of qdh to nearly homogeneity was achieved. the purified qdh appears to have two subunits of approximately 65 and 21 kda, which could be the result of proteolysis of single polypeptide. kinetic an ...	2004	15598527
identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases.	selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (ahs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. first, substrate range mutants of the pseudomonas putida gpo1 and alcanivorax borkumensis ap1 medium-chain-length ahs were obtained by selection experiments with a specially constructed host. in all mutants able to oxidize alkanes longer than c13, w55 (in the case of p. put ...	2005	15601691
impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene.	with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ...	2005	15626648
impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene.	with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ...	2005	15626648
[effect of delay of blood cultures on positive detection by automated blood culture system].	the effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; bactec 9240 system and bact/alert 3d system. total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. bactec 92f, 93f and bact/alert fa, fn bottles were used as the blood culture bottles. all the bottles inoc ...	2004	15628528
[bacterial causes of meningitis in newborns].	bacterial meningitis jeopardizes the life of affected newborns and in survived children often leaves permanent sequels. the sooner the diagnosis is established and the therapy is started, the more a prosperous outcome can be expected. when the indication is established, lumbar punction should be performed. on the basis of the results of cytological and biochemical analyses of the cerebrospinal fluid (csf) that point to bacterial meningitis, the initial therapy should begin even before the result ...	2001	15637989
manual and automated instrumentation for identification of enterobacteriaceae and other aerobic gram-negative bacilli.	identification of gram-negative bacilli, both enteric and nonenteric, by conventional methods is not realistic for clinical microbiology laboratories performing routine cultures in today's world. the use of commercial kits, either manual or automated, to identify these organisms is a common practice. the advent of rapid or "spot" testing has eliminated the need for some commonly isolated organisms to be identified with the systems approach. commercially available systems provide more in-depth id ...	2005	15653824
mechanism of lipid-body formation in prokaryotes: how bacteria fatten up.	neutral lipid accumulation is frequently observed in some gram-negative prokaryotes like acinetobacter sp. and most actinomycetes, including the pathogenic mycobacterium tuberculosis and antibiotic producing streptomycetes. we examined the formation of wax ester- and triacylglycerol (tag)-bodies in acinetobacter calcoaceticus and rhodococcus opacus using microscopic, immunological and biophysical methods. a general model for prokaryotic lipid-body formation is proposed, clearly differing from th ...	2005	15661001
thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme a:diacylglycerol acyltransferase of acinetobacter sp. strain adp1.	the bifunctional wax ester synthase/acyl coenzyme a (acyl-coa):diacylglycerol acyltransferase (ws/dgat) from acinetobacter sp. strain adp1 (formerly acinetobacter calcoaceticus adp1) mediating the biosyntheses of wax esters and triacylglycerols was used for the in vivo and in vitro biosynthesis of thio wax esters and dithio wax esters. for in vitro biosynthesis, 5'his(6)ws/dgat comprising an n-terminal his(6) tag was purified from the soluble protein fraction of escherichia coli rosetta(de3)plys ...	2005	15691932
physiological conditions conducive to high cyanophycin content in biomass of acinetobacter calcoaceticus strain adp1.	the effects of the inorganic medium components, the initial ph, the incubation temperature, the oxygen supply, the carbon-to-nitrogen ratio, and chloramphenicol on the synthesis of cyanophycin (cgp) by acinetobacter calcoaceticus strain adp1 were studied in a mineral salts medium containing sodium glutamate and ammonium sulfate as carbon and nitrogen sources, respectively. variation of all these factors resulted in maximum cgp contents of only about 3.5% (wt/wt) of the cell dry matter (cdm), and ...	2005	15691941
transcriptional organization of genes for protocatechuate and quinate degradation from acinetobacter sp. strain adp1.	quinate and protocatechuate are both abundant plant products and can serve, along with a large number of other aromatic or hydroaromatic compounds, as growth substrates for acinetobacter sp. strain adp1. the respective genes are part of the chromosomal dca-pca-qui-pob-hca cluster encoding these pathways. the adjacent pca and qui gene clusters, which encode enzymes for protocatechuate breakdown via the beta-ketoadipate pathway and for the conversion of quinate or shikimate to protocatechuate, res ...	2005	15691962
response of biofilm bacteria to dissolved organic matter from decomposing maple leaves.	stream bacteria play an important role in the utilization of dissolved organic matter (dom) leached from leaves, and in transfer of this dom to other trophic levels. leaf leachate is a mixture of labile, recalcitrant, and inhibitory compounds, and bacterial communities vary in their ability to utilize leachate. the purpose of this study was to determine the effects of dom from sugar maple leaves on bacterial populations in biofilms on decomposing leaf surfaces. populations of acinetobacter calco ...	2004	15692852
increasing stability of water-soluble pqq glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface.	water-soluble quinoprotein glucose dehydrogenase (pqqgdh-b) from acinetobacter calcoaceticus has a great potential for application as a glucose sensor constituent. because this enzyme shows no activity in its monomeric form, correct quaternary structure is essential for the formation of active enzyme. we have previously reported on the increasing of the stability of pqqgdh-b by preventing the subunit dissociation. previous studies were based on decreasing the entropy of quaternary structure diss ...	2005	15715904
epr studies of chlorocatechol 1,2-dioxygenase: evidences of iron reduction during catalysis and of the binding of amphipatic molecules.	chlorocatechol 1,2-dioxygenase from pseudomonas putida (pp 1,2-ccd) is a dioxygenase responsible for ring cleavage during the degradation of recalcitrant aromatic compounds. we determined the zero-field splitting of the fe(iii) cofactor (\|d\| = 1.3 +/- 0.2 cm(-1)) by electron paramagnetic resonance (epr) experiments that along with other structural data allowed us to infer the fe(iii) coordination environment. the epr spectrum of the ion shows a significantly decrease of the g = 4.3 resonance upo ...	2005	15722436
development of a novel glucose enzyme fuel cell system employing protein engineered pqq glucose dehydrogenase.	glucose dehydrogenase harboring pyrroloquinoline quinone as the prosthetic group (pqqgdh) from acinetobacter calcoaceticus is an ideal enzyme for the anode of biofuel cell, because of its oxygen insensitivity and high catalytic efficiency. however, the application of pqqgdh for the bioanode is inherently limited because of its instability. using ser415cys mutant whose stability was greatly improved, we constructed the biofuel cell system employing the engineered pqqgdh as the bioanode enzyme and ...	2005	15741089
quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia.	a quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-rflp) assay, was developed for simultaneous determination of microbial diversity and abundance within a complex community. the real-time-t-rflp assay was developed by incorporating the quantitative feature of real-time pcr and the fingerprinting feature of t-rflp analysis. the assay was validated by using a model microbial community containing three pure strains, an escherich ...	2005	15746346
biosorption and recycling of gold using various microorganisms.	in order to obtain basic information on the biosorption and recycling of gold from aqueous systems using microbial cells, the biosorption of gold by various microorganisms was investigated. of 75 strains of microorganisms tested (25 bacteria, 19 actinomycetes, 17 fungi and 14 yeasts), high abilities of gold biosorption from a solution containing hydrogen tetrachloroaurate (iii) were found in some gram-negative bacterial strains, such as acinetobacter calcoaceticus, erwinia herbicola, pseudomonas ...	2004	15754248
detection of and response to signals involved in host-microbe interactions by plant-associated bacteria.	diverse interactions between hosts and microbes are initiated by the detection of host-released chemical signals. detection of these signals leads to altered patterns of gene expression that culminate in specific and adaptive changes in bacterial physiology that are required for these associations. this concept was first demonstrated for the members of the family rhizobiaceae and was later found to apply to many other plant-associated bacteria as well as to microbes that colonize human and anima ...	2005	15755957
alkane-induced expression, substrate binding profile, and immunolocalization of a cytochrome p450 encoded on the nifd excision element of anabaena 7120.	alkanes have been hypothesized to act as universal inducers of bacterial cytochrome p450 gene expression. we tested this hypothesis on an unusual p450 gene (cyp110) found on a conserved 11 kilobase episomal dna element of unknown function found in filamentous cyanobacteria. we also monitored the binding of potential substrates to the p450 protein and explored the distribution of p450 protein in vegetative cells and nitrogen-fixing heterocysts using immuno-electron microscopy.	2005	15790415
[use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil].	oil-oxidizing bacteria were isolated from oil-polluted soil and water samples and identified as acinetobacter calcoaceticus k-4, nocardia vaceinii k-8, rhodococcus erythropolis ek-1, and mycobacterium sp. k-2. it was found that immobilization of the bacteria on an expanded clay aggregate accelerated their growth and consumption of hydrocarbon substrates. it was also found that water polluted with 100 mg/l oil could be purified with rhodococcus erythropolis ek-1 and nocardia vaceinii k-8 cells im ...	2005	15810734
biocatalytic production of perillyl alcohol from limonene by using a novel mycobacterium sp. cytochrome p450 alkane hydroxylase expressed in pseudomonas putida.	a number of oxygenated monoterpenes present at low concentrations in plant oils have anticarcinogenic properties. one of the most promising compounds in this respect is (-)-perillyl alcohol. since this natural product is present only at low levels in a few plant oils, an alternative, synthetic source is desirable. screening of 1,800 bacterial strains showed that many alkane degraders were able to specifically hydroxylate l-limonene in the 7 position to produce enantiopure (-)-perillyl alcohol. t ...	2005	15811996
formation of trans fatty acids is not involved in growth-linked membrane adaptation of pseudomonas putida.	fatty acid compositions in growing and resting cells of several strains of pseudomonas putida (p8, nctc 10936, and kt 2440) were studied, with a focus on alterations of the saturation degree, cis-trans isomerization, and cyclopropane formation. the fatty acid compositions of the strains were very similar under comparable growth conditions, but surprisingly, and contrary to earlier reports, trans fatty acids were not found in either exponentially growing cells or stationary-phase cells. during th ...	2005	15812020
novel major bacterial candidate division within a municipal anaerobic sludge digester.	in a previous study, we analyzed the molecular diversity of planctomycetales by pcr amplification and sequencing of 16s rrna clone libraries generated from a municipal wastewater plant, using planctomycete-specific and universal primer sets (r. chouari, d. le paslier, p. daegelen, p. ginestet, j. weissenbach, and a. sghir, appl. environ. microbiol. 69:7354-7363, 2003). only a small fraction (4%) of the 16s rrna gene sequences of the digester clone library corresponded to the planctomycetales div ...	2005	15812049
species-level identification of isolates of the acinetobacter calcoaceticus-acinetobacter baumannii complex by sequence analysis of the 16s-23s rrna gene spacer region.	the species acinetobacter calcoaceticus, a. baumannii, genomic species 3, and genomic species 13tu included in the acinetobacter calcoaceticus-acinetobacter baumannii complex are genetically highly related and difficult to distinguish phenotypically. except for a. calcoaceticus, they are all important nosocomial species. in the present study, the usefulness of the 16s-23s rrna gene intergenic spacer (its) sequence for the differentiation of (genomic) species in the a. calcoaceticus-a. baumannii ...	2005	15814977
sulfoacetaldehyde is excreted quantitatively by acinetobacter calcoaceticus sw1 during growth with taurine as sole source of nitrogen.	eighteen enrichment cultures with taurine (2-aminoethanesulfonate) as the sole source of combined nitrogen under aerobic conditions were all successful, and 24 pure cultures were obtained. only three of the cultures yielded an inorganic product, sulfate, from the sulfonate moiety of taurine, and the others were presumed to yield organosulfonates. sulfoacetate, known from rhodopseudomonas palustris cga009 under these conditions, was not detected in any culture, but sulfoacetaldehyde (as a hydrazo ...	2005	15817795
structural modifications and antimicrobial activity of n-cycloalkenyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles.	a series of n-cycloalkenyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles 5a-j, n-cycloalkyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles 8a-e, and n-alkyl-2-acylalkylidene-2,3-dihydro-1,3-benzothiazoles 8f-h, were synthesized and tested for in vitro antibacterial and antifungal activities against four gram-positive and five gram-negative bacteria (bacillus subtilis 6633, enterococcus faecalis 29212, staphylococcus aureus 6538, staphylococcus aureus 25923, escherichia coli 25922, acinetobact ...	2005	15848203
incidence of class 1 integrons in a quaternary ammonium compound-polluted environment.	samples of effluent and soil were collected from a reed bed system used to remediate liquid waste from a wool finishing mill with a high use of quaternary ammonium compounds (qacs) and were compared with samples of agricultural soils. resistance quotients of aerobic gram-negative and gram-positive bacteria to ditallowdimethylammomium chloride (dtdmac) and cetyltrimethylammonium bromide (ctab) were established by plating onto nutrient agar containing 5 microg/ml or 50 microg/ml dtdmac or ctab. ap ...	2005	15855499
seasonal response of stream biofilm communities to dissolved organic matter and nutrient enrichments.	dissolved organic matter (dom) and inorganic nutrients may affect microbial communities in streams, but little is known about the impact of these factors on specific taxa within bacterial assemblages in biofilms. in this study, nutrient diffusing artificial substrates were used to examine bacterial responses to dom (i.e., glucose, leaf leachate, and algal exudates) and inorganic nutrients (nitrate and phosphate singly and in combination). artificial substrates were deployed for five seasons, fro ...	2005	15870312
changes in antibiotic resistance of the most common gram-negative bacteria isolated in intensive care units.	we studied the changes in antibiotic resistance of the most common gram-negative bacteria isolated in the intensive care units at our hospital in 2000 and 2002. bacterial identification was performed by use of the vitek 60 analyser, and antibiotic susceptibilities were tested by the vitek 60 analyser and the disk diffusion agar method. the bacteria isolated most frequently were pseudomonas aeruginosa (132 strains in 2000 and 106 in 2002), acinetobacter calcoaceticus (98 and 109 strains, respecti ...	2005	15890431
neutral lipid bodies in prokaryotes: recent insights into structure, formation, and relationship to eukaryotic lipid depots.		2005	15901682