Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| differential biofilm formation and motility associated with lipopolysaccharide/exopolysaccharide-coupled biosynthetic genes in stenotrophomonas maltophilia. | stenotrophomonas maltophilia wr-c is capable of forming biofilm on polystyrene and glass. the lipopolysaccharide/exopolysaccharide-coupled biosynthetic genes rmla, rmlc, and xanb are necessary for biofilm formation and twitching motility. mutants with mutations in rmlac and xanb display contrasting biofilm phenotypes on polystyrene and glass and differ in swimming motility. | 2006 | 16585771 |
| gene-for-gene interactions of five cloned avirulence genes from xanthomonas campestris pv. malvacearum with specific resistance genes in cotton. | a total dna clone bank of a strain of xanthomonas campestris pv. malvacearum (xcm) was constructed in the cosmid vector psa747 and transfected into escherichia coli. the xcm strain carries at least nine identifiable avirulence (a) genes. clones in e. coli were mated individually into a recombination-proficient xcm isolate carrying no known a genes. screening was for incompatibility on congenic cotton host lines that differ by single specific resistance (r) genes. ten different cosmid clones conf ... | 1986 | 16593751 |
| characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean. | xanthomonas campestris pv. vesicatoria is the causal agent of leaf spot disease on pepper and tomato. on non-host plants, such as bean, soybean, cowpea, alfalfa, and cotton, x. campestris pv. vesicatoria is unable to cause disease, inducing instead a hypersensitive resistance response (hr). since avirulence genes from x. campestris pv. vesicatoria specifically induce hr in several pepper cultivars, we investigated whether there were avirulence genes governing induction of resistance in non-host ... | 1988 | 16593982 |
| use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of burkholderia pseudomallei. | melioidosis, a disease caused by the bacterium burkholderia pseudomallei, is endemic in southeast asia and northern australia. we used suppression subtractive hybridization (ssh) to identify sequences that varied between two b. pseudomallei isolates from australia and determined the distribution of 45 ssh-derived sequences among a panel of b. pseudomallei and b. thailandensis isolates. sequences exhibiting variable prevalence were included in a variable amplicon typing (vat) scheme designed to s ... | 2006 | 16597858 |
| assessment of the environmental fate of the biological control agent of fire blight, pseudomonas fluorescens eps62e, on apple by culture and real-time pcr methods. | the colonization of apple blossoms and leaves by pseudomonas fluorescens eps62e was monitored in greenhouse and field trials using cultivable cell counting and real-time pcr. the real-time pcr provided a specific quantitative method for the detection of strain eps62e. the detection level was around 10(2) cells g (fresh weight)(-1) and the standard curve was linear within a 5-log range. eps62e actively colonized flowers reaching values from 10(7) to 10(8) cells per blossom. in apple flowers, no s ... | 2006 | 16597940 |
| quantitative real-time legionella pcr for environmental water samples: data interpretation. | quantitative legionella pcrs targeting the 16s rrna gene (specific for the genus legionella) and the mip gene (specific for the species legionella pneumophila) were applied to a total of 223 hot water system samples (131 in one laboratory and 92 in another laboratory) and 37 cooling tower samples (all in the same laboratory). the pcr results were compared with those of conventional culture. 16s rrna gene pcr results were nonquantifiable for 2.8% of cooling tower samples and up to 39.1% of hot wa ... | 2006 | 16597985 |
| a study on the molecular mechanism of resistance to amicarthiazol in xanthomonas campestris pv. citri. | three amicarthiazol-resistant mutants (xuv10, xuv20 and xuv40) were obtained by uv induction and used in this study. minimal inhibition concentrations (mics) of amicarthiazol against the growth of mutants and wild-type isolate were 400 and 100 microg ml(-1) respectively. inhibition by amicarthiazol of succinate dehydrogenase (sdh) activities of xanthomonas campestris pv. citri (hasse) dye wild-type isolate (xcc) and three resistant mutants derived from this isolate were assayed using triphenylte ... | 2006 | 16602085 |
| identification of the bacterial superoxide dismutase (sodm) as plant-inducible elicitor of an oxidative burst reaction in tobacco cell suspension cultures. | three of the most abundant proteins (ompw, mopb and sodm) of the extracellular proteome of xanthomonas campestris pv. campestris were analysed in a luminol-based oxidative burst assay to identify novel pathogen-associated molecular patterns (pamp). tobacco cell suspension cultures were used as a model system to monitor elicitor induced plant defence reaction. the candidate proteins were isolated from two-dimensional gels prior to application to the oxidative burst assay. the superoxide dismutase ... | 2006 | 16603270 |
| talking to themselves: autoregulation and quorum sensing in fungi. | 2006 | 16607008 | |
| mekhla, a novel domain with similarity to pas domains, is fused to plant homeodomain-leucine zipper iii proteins. | homeodomain (hd) proteins play important roles in the development of plants, fungi, and animals. here we identify a novel domain, mekhla, in the c terminus of hd-leu zipper (hd-zip) iii plant hd proteins that shares similarity with a group of bacterial proteins and a protein from the green alga chlamydomonas reinhardtii. the group of bacterial mekhla proteins is found in cyanobacteria and other bacteria often found associated with plants. phylogenetic analysis suggests that a mekhla protein tran ... | 2006 | 16607028 |
| cell-cell signaling in xanthomonas campestris involves an hd-gyp domain protein that functions in cyclic di-gmp turnover. | hd-gyp is a protein domain of unknown biochemical function implicated in bacterial signaling and regulation. in the plant pathogen xanthomonas campestris pv. campestris, the synthesis of virulence factors and dispersal of biofilms are positively controlled by a two-component signal transduction system comprising the hd-gyp domain regulatory protein rpfg and cognate sensor rpfc and by cell-cell signaling mediated by the diffusible signal molecule dsf (diffusible signal factor). the rpfg/rpfc two- ... | 2006 | 16611728 |
| pathogen detection, testing, and control in fresh broccoli sprouts. | the recent increased interest in consuming green vegetable sprouts has been tempered by the fact that fresh sprouts can in some cases be vehicles for food-borne illnesses. they must be grown according to proper conditions of sanitation and handled as a food product rather than as an agricultural commodity. when sprouts are grown in accordance with the criteria proposed from within the sprout industry, developed by regulatory agencies, and adhered to by many sprouters, green sprouts can be produc ... | 2006 | 16630354 |
| subcellular localization and functions of the barley stem rust resistance receptor-like serine/threonine-specific protein kinase rpg1. | the rpg1 gene confers resistance to many pathotypes of the stem rust fungus puccinia graminis f. sp. tritici and has protected barley from serious disease losses for over 60 years. rpg1 encodes a constitutively expressed protein with two tandem kinase domains. fractionation by differential centrifugation and aqueous two-phase separation of the microsome proteins located rpg1 mainly in the cytosol but also in the plasma membrane and intracellular membranes. recombinant rpg1 autophosphorylates in ... | 2006 | 16648249 |
| promoter mutations of an essential gene for pollen development result in disease resistance in rice. | disease resistance and sexual reproductive development are generally considered as separate biological processes, regulated by different sets of genes. here we show that xa13, a recessive allele conferring disease resistance against bacterial blight, one of the most devastating rice diseases worldwide, plays a key role in both disease resistance and pollen development. the dominant allele, xa13, is required for both bacterial growth and pollen development. promoter mutations in xa13 cause down-r ... | 2006 | 16648463 |
| genome-wide prediction of g4 dna as regulatory motifs: role in escherichia coli global regulation. | the role of nonlinear dna in replication, recombination, and transcription has become evident in recent years. although several studies have predicted and characterized regulatory elements at the sequence level, very few have investigated dna structure as regulatory motifs. here, using g-quadruplex or g4 dna motifs as a model, we have researched the role of dna structure in transcription on a genome-wide scale. analyses of >61,000 open reading frames (orfs) across 18 prokaryotes show enrichment ... | 2006 | 16651665 |
| cotton fleahopper and associated microorganisms as components in the production of stress ethylene by cotton. | excised cotton terminal buds incubated with adults or nymphs of the cotton fleahopper (cfh), pseudatomoscelis seriatus (reuter), produced ethylene at theoretical abscission-inducing rates by 24 h after introduction of the insect. inoculation of cotton shoot tips with three microorganisms commonly associated with cfh and cotton in all cases promoted ethylene production to theoretical abscission-inducing rates by 24 h after inoculation. cfh alone or injection of microorganisms consistently caused ... | 1988 | 16666119 |
| induction of hydrolytic enzymes in brassica campestris in response to pathovars of xanthomonas campestris. | inoculation of mature leaves of turnip (brassica campestris) with the incompatible xanthomonas campestris pv vitians resulted in the induction of beta-1,3-glucanase and chitinase/lysozyme (chl) activity. no increase in the basal activity of beta-1,3-glucanase was observed after inoculation of leaves with heat- or rifampicin-killed x. c. vitians, escherichia coli, or sterile water. inoculation with the compatible x. campestris pv campestris resulted in a slower induction of glucanase than that se ... | 1990 | 16667441 |
| phytoalexin accumulation in arabidopsis thaliana during the hypersensitive reaction to pseudomonas syringae pv syringae. | inoculation of leaves of arabidopsis thaliana (l.) heynh. with the wheat pathogen, pseudomonas syringae pv syringae, resulted in the expression of the hypersensitive reaction and in phytoalexin accumulation. no phytoalexin accumulation was detected after infiltration of leaves with a mutant of p. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, xanthomonas campestris pv campestris; or with 10 millimolar potassium phosphate buffer (ph 6.9). phy ... | 1992 | 16668792 |
| survival strategy of erwinia amylovora against copper: induction of the viable-but-nonculturable state. | copper compounds, widely used to control plant-pathogenic bacteria, have traditionally been employed against fire blight, caused by erwinia amylovora. however, recent studies have shown that some phytopathogenic bacteria enter into the viable-but-nonculturable (vbnc) state in the presence of copper. to determine whether copper kills e. amylovora or induces the vbnc state, a mineral medium without copper or supplemented with 0.005, 0.01, or 0.05 mm cu(2+) was inoculated with 10(7) cfu/ml of this ... | 2006 | 16672494 |
| an outer membrane enzyme encoded by salmonella typhimurium lpxr that removes the 3'-acyloxyacyl moiety of lipid a. | the salmonella and related bacteria modify the structure of the lipid a portion of their lipopolysaccharide in response to environmental stimuli. some lipid a modifications are required for virulence and resistance to cationic antimicrobial peptides. we now demonstrate that membranes of salmonella typhimurium contain a novel hydrolase that removes the 3'-acyloxyacyl residue of lipid a in the presence of 5 mm ca2+. we have identified the gene encoding the s. typhimurium lipid a 3'-o-deacylase, de ... | 2006 | 16704973 |
| a multiplex real-time pcr assay for detection of xanthomonas campestris from brassicas. | to develop a sensitive real-time pcr-based protocol for the detection of xanthomonas campestris pathovars from brassica seed. | 2006 | 16706903 |
| saccharomyces cerevisiae cells have three omega class glutathione s-transferases acting as 1-cys thiol transferases. | the saccharomyces cerevisiae genome encodes three proteins that display similarities with human gstos (omega class glutathione s-transferases) hgsto1-1 and hgsto2-2. the three yeast proteins have been named gto1, gto2 and gto3, and their purified recombinant forms are active as thiol transferases (glutaredoxins) against hed (beta-hydroxyethyl disulphide), as dehydroascorbate reductases and as dimethylarsinic acid reductases, while they are not active against the standard gst substrate cdnb (1-ch ... | 2006 | 16709151 |
| functional roles of the pepper pathogen-induced bzip transcription factor, cabzip1, in enhanced resistance to pathogen infection and environmental stresses. | transcription factors often belong to multigene families and their individual contribution in a particular regulatory network remains difficult to assess. we identify and functionally characterize the pepper bzip transcription factor cabzip1 gene isolated from pepper leaves infected with xanthomonas campestris pv. vesicatoria. transient expression analysis of the cabzip1-gfp fusion protein in arabidopsis protoplasts revealed that the cabzip1 protein is localized in the nucleus. the n-terminal re ... | 2006 | 16718483 |
| evolution of a signalling system that incorporates both redundancy and diversity: arabidopsis sumoylation. | the reversible post-translational modifier, sumo (small ubiquitin-related modifier), modulates the activity of a diverse set of target proteins, resulting in important consequences to the cellular machinery. conjugation machinery charges the processed sumo so that it can be linked via an isopeptide bond to a target protein. the removal of sumo moieties from conjugated proteins by isopeptidases regenerates pools of processed sumos and unmodified target proteins. the evolutionarily conserved sumo- ... | 2006 | 16740136 |
| structural classification of bacterial response regulators: diversity of output domains and domain combinations. | chey-like phosphoacceptor (or receiver [rec]) domain is a common module in a variety of response regulators of the bacterial signal transduction systems. in this work, 4,610 response regulators, encoded in complete genomes of 200 bacterial and archaeal species, were identified and classified by their domain architectures. previously uncharacterized output domains were analyzed and, in some cases, assigned to known domain families. transcriptional regulators of the ompr, narl, and ntrc families w ... | 2006 | 16740923 |
| structures of n-acetylornithine transcarbamoylase from xanthomonas campestris complexed with substrates and substrate analogs imply mechanisms for substrate binding and catalysis. | n-acetyl-l-ornithine transcarbamoylase (aotcase) is a new member of the transcarbamoylase superfamily that is essential for arginine biosynthesis in several eubacteria. we report here crystal structures of the binary complexes of aotcase with its substrates, carbamoyl phosphate (cp) or n-acetyl-l-ornithine (aorn), and the ternary complex with cp and n-acetyl-l-norvaline. comparison of these structures demonstrates that the substrate-binding mechanism of this novel transcarbamoylase is different ... | 2006 | 16741992 |
| structure of a novel n-acetyl-l-citrulline deacetylase from xanthomonas campestris. | the structure of a novel acetylcitrulline deacetylase from the plant pathogen xanthomonas campestris has been solved by multiple-wavelength anomalous dispersion (mad) using crystals grown from selenomethionine-substituted protein and refined at 1.75 a resolution. the asymmetric unit of the crystal contains one monomer consisting of two domains, a catalytic domain and a dimerization domain. the catalytic domain is able to bind a single co(ii) ion at the active site with no change in conformation. ... | 2007 | 16750290 |
| succession of bacterial communities during early plant development: transition from seed to root and effect of compost amendment. | compost amendments to soils and potting mixes are routinely applied to improve soil fertility and plant growth and health. these amendments, which contain high levels of organic matter and microbial cells, can influence microbial communities associated with plants grown in such soils. the purpose of this study was to follow the bacterial community compositions of seed and subsequent root surfaces in the presence and absence of compost in the potting mix. the bacterial community compositions of p ... | 2006 | 16751505 |
| construction of phylogenetic trees by kernel-based comparative analysis of metabolic networks. | to infer the tree of life requires knowledge of the common characteristics of each species descended from a common ancestor as the measuring criteria and a method to calculate the distance between the resulting values of each measure. conventional phylogenetic analysis based on genomic sequences provides information about the genetic relationships between different organisms. in contrast, comparative analysis of metabolic pathways in different organisms can yield insights into their functional r ... | 2006 | 16753070 |
| structure of xc6422 from xanthomonas campestris at 1.6 a resolution: a small serine alpha/beta-hydrolase. | xc6422 is a conserved hypothetical protein from xanthomonas campestris pathovar campestris (xcc), a gram-negative yellow-pigmented pathogenic bacterium that causes black rot, one of the major worldwide diseases of cruciferous crops. the protein consists of 220 amino acids and its structure has been determined to 1.6 a resolution using the multi-wavelength anomalous dispersion (mad) method. although it has very low sequence identity to protein sequences in the pdb (less than 20%), the determined ... | 2006 | 16754966 |
| iscr elements: novel gene-capturing systems of the 21st century? | "common regions" (crs), such as orf513, are being increasingly linked to mega-antibiotic-resistant regions. while their overall nucleotide sequences show little identity to other mobile elements, amino acid alignments indicate that they possess the key motifs of is91-like elements, which have been linked to the mobility ent plasmids in pathogenic escherichia coli. further inspection reveals that they possess an is91-like origin of replication and termination sites (teris), and therefore crs prob ... | 2006 | 16760305 |
| crystal structure of a putative acyl-coa thioesterase from xanthomonas campestris (xc229) adopts a tetrameric hotdog fold of epsilongamma mode. | 2006 | 16763992 | |
| characteristics of transgenic tomatoes antisensed for the ethylene receptor genes leetr1 [corrected] and leetr2 [corrected]. | two stable transformed lines containing antisense leetr1 [corrected] or leetr2 [corrected] sequences and their hybridized line were investigated to determine the effect of leetr1 [corrected] and leetr2 [corrected] specificity in the ethylene receptor family in tomato (lycopersicon esculentum mill.) on ethylene signaling. the transgenic line ale1 containing antisense leetr1 [corrected] displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, a ... | 2006 | 16773735 |
| activation of the global gene regulator prra (rega) from rhodobacter sphaeroides. | prra is a global transcription regulator activated upon phosphorylation by its cognate kinase prrb in response to low oxygen levels in rhodobacter sphaeroides. here we show by gel filtration, analytical ultracentrifugation, and nmr diffusion measurements that treatment of prra with a phosphate analogue, bef(3)(-), results in dimerization of the protein, producing a protein that binds dna. no dimeric species was observed in the absence of bef(3)(-). upon addition of bef(3)(-), the inhibitory acti ... | 2006 | 16784239 |
| popf1 and popf2, two proteins secreted by the type iii protein secretion system of ralstonia solanacearum, are translocators belonging to the hrpf/nopx family. | ralstonia solanacearum gmi1000 is a gram-negative plant pathogen which contains an hrp gene cluster which codes for a type iii protein secretion system (ttss). we identified two novel hrp-secreted proteins, called popf1 and popf2, which display similarity to one another and to putative ttss translocators, hrpf and nopx, from xanthomonas spp. and rhizobia, respectively. they also show similarities with ttss translocators of the yopb family from animal-pathogenic bacteria. both popf1 and popf2 bel ... | 2006 | 16788199 |
| prrc-anticodon nuclease: functional organization of a prototypical bacterial restriction rnase. | the trna(lys) anticodon nuclease prrc is associated in latent form with the type ic dna restriction endonuclease ecoprri and activated by a phage t4-encoded inhibitor of ecoprri. the activation also requires the hydrolysis of gtp and presence of dttp and is inhibited by atp. the n-proximal ntpase domain of prrc has been implicated in relaying the activating signal to a c-proximal anticodon nuclease site by interacting with the requisite nucleotide cofactors [amitsur et al. (2003) mol. microbiol. ... | 2006 | 16790566 |
| impaired photosynthesis in phosphatidylglycerol-deficient mutant of cyanobacterium anabaena sp. pcc7120 with a disrupted gene encoding a putative phosphatidylglycerophosphatase. | phosphatidylglycerol (pg) is a ubiquitous phospholipid in thylakoid membranes of cyanobacteria and chloroplasts and plays an important role in the structure and function of photosynthetic membranes. the last step of the pg biosynthesis is dephosphorylation of phosphatidylglycerophosphate (pgp) catalyzed by pgp phosphatase. however, the gene-encoding pgp phosphatase has not been identified and cloned from cyanobacteria or higher plants. in this study, we constructed a pg-deficient mutant from cya ... | 2006 | 16815953 |
| the brucella abortus cyclic beta-1,2-glucan virulence factor is substituted with o-ester-linked succinyl residues. | brucella periplasmic cyclic beta-1,2-glucan plays an important role during bacterium-host interaction. nuclear magnetic resonance spectrometry analysis, thin-layer chromatography, and deae-sephadex chromatography were used to characterize brucella abortus cyclic glucan. in the present study, we report that a fraction of b. abortus cyclic beta-1,2-glucan is substituted with succinyl residues, which confer anionic character on the cyclic beta-1,2-glucan. the oligosaccharide backbone is substituted ... | 2006 | 16816173 |
| surface display of transglucosidase on escherichia coli by using the ice nucleation protein of xanthomonas campestris and its application in glucosylation of hydroquinone. | a surface anchoring motif using the ice nucleation protein (inp) of xanthomonas campestris pv. campestris bcrc 12,846 for display of transglucosidase has been developed. the transglucosidase gene from xanthomonas campestris pv. campestris bcrc 12,608 was fused to the truncated ina gene. this truncated inp consisting of n- and c-terminal domains (inpnc) was able to direct the expressed transglucosidase fusion protein to the cell surface of e. coli with apparent high enzymatic activity. the locali ... | 2006 | 16817238 |
| herbivore-induced resistance against microbial pathogens in arabidopsis. | caterpillars of the herbivore pieris rapae stimulate the production of jasmonic acid (ja) and ethylene (et) in arabidopsis (arabidopsis thaliana) and trigger a defense response that affects insect performance on systemic tissues. to investigate the spectrum of effectiveness of p. rapae-induced resistance, we examined the level of resistance against different pathogens. although the necrotrophic fungus alternaria brassicicola is sensitive to ja-dependent defenses, herbivore-induced resistance was ... | 2006 | 16829584 |
| distinct domains in the arc region of the potato resistance protein rx mediate lrr binding and inhibition of activation. | plant nucleotide binding and leucine-rich repeat (nb-lrr) proteins contain a region of homology known as the arc domain located between the nb and lrr domains. structural modeling suggests that the arc region can be subdivided into arc1 and arc2 domains. we have used the potato (solanum tuberosum) rx protein, which confers resistance to potato virus x (pvx), to investigate the function of the arc region. we demonstrate that the arc1 domain is required for binding of the rx n terminus to the lrr ... | 2006 | 16844906 |
| molecules involved in the modulation of rapid cell death in xanthomonas. | in earlier studies from this laboratory, xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (rcd). the rcd was exhibited in protein-rich media but not in starch or other minimal media. this rcd in x. campestris pv. glycines was found to display features similar to those of the programmed cell death (pcd) of eukaryotes. results of the present study showed that the observed rcd in this organism is both positively and negatively regu ... | 2006 | 16855230 |
| the prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like beta-grasp domains. | ubiquitin (ub)-mediated signaling is one of the hallmarks of all eukaryotes. prokaryotic homologs of ub (this and moad) and e1 ligases have been studied in relation to sulfur incorporation reactions in thiamine and molybdenum/tungsten cofactor biosynthesis. however, there is no evidence for entire protein modification systems with ub-like proteins and deconjugation by deubiquitinating enzymes in prokaryotes. hence, the evolutionary assembly of the eukaryotic ub-signaling apparatus remains unclea ... | 2006 | 16859499 |
| metabolic engineering of microbes for oligosaccharide and polysaccharide synthesis. | metabolic engineering has recently been embraced as an effective tool for developing whole-cell biocatalysts for oligosaccharide and polysaccharide synthesis. microbial catalysts now provide a practical means to derive many valuable oligosaccharides, previously inaccessible through other methods, in sufficient quantities to support research and clinical applications. the synthesis process based upon these microbes is scalable as it avoids expensive starting materials. most impressive is the high ... | 2006 | 16859553 |
| the crystal structure of xc1739: a putative multiple antibiotic-resistance repressor (marr) from xanthomonas campestris at 1.8 a resolution. | 2006 | 16862595 | |
| physical delimitation of the pepper bs3 resistance gene specifying recognition of the avrbs3 protein from xanthomonas campestris pv. vesicatoria. | the pepper (capsicum annuum) bs3 gene confers resistance to avrbs3-expressing strains of the bacterial spot pathogen xanthomonas campestris pv. vesicatoria. to physically delimit bs3, a pepper yac library was screened with two flanking dna markers that are separated from bs3 by 1.0 and 1.2 cm, respectively resulting in the identification of three yac clones. genetic mapping of the corresponding yacends revealed however, that these yacs do not cover bs3 and subsequent screens with newly developed ... | 2006 | 16874489 |
| protein-translocating trimeric autotransporters of gram-negative bacteria. | 2006 | 16885434 | |
| activity of rhodobacter sphaeroides rpohii, a second member of the heat shock sigma factor family. | we have identified a second rpoh homolog, rpoh(ii), in the alpha-proteobacterium rhodobacter sphaeroides. primary amino acid sequence comparisons demonstrate that r. sphaeroides rpoh(ii) belongs to a phylogenetically distinct group with rpoh orthologs from alpha-proteobacteria that contain two rpoh genes. like its previously identified paralog, rpoh(i), rpoh(ii) is able to complement the temperature-sensitive phenotype of an escherichia coli sigma(32) (rpoh) mutant. in addition, we show that rec ... | 2006 | 16885439 |
| global gene expression analysis of the heat shock response in the phytopathogen xylella fastidiosa. | xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. although different strains have been studied, little is known about x. fastidiosa stress responses. one of the better characterized stress responses in bacteria is the heat shock response, which induces the expression of specific genes to prevent protein misfolding and aggregation and to promote degradation of the irreversibly denatured polypeptides. to investigate x. fastidio ... | 2006 | 16885450 |
| identification and functional expression of the pepper pathogen-induced gene, capip2, involved in disease resistance and drought and salt stress tolerance. | a novel pathogen-induced gene, designated capip2, was isolated from pepper leaves infected with xanthomonas campestris pv. vesicatoria. capip2:gfp fusion proteins were primarily localized in the cytoplasm. the capip2 transcripts were constitutively expressed in the pepper leaves, flowers, and fruits, but were not detected in the stems and roots. capip2 gene expression was induced strongly in the pepper leaves during pathogen infection, and also after exposure to abiotic elicitors and environment ... | 2006 | 16900319 |
| random mutagenesis in corynebacterium glutamicum atcc 13032 using an is6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway. | corynebacterium glutamicum, a gram-positive bacterium of the class actinobacteria, is an industrially relevant producer of amino acids. several methods for the targeted genetic manipulation of this organism and rational strain improvement have been developed. an efficient transposon mutagenesis system for the completely sequenced type strain atcc 13032 would significantly advance functional genome analysis in this bacterium. | 2006 | 16901339 |
| challenging xanthomonas campestris with low levels of arsenic mediates cross-protection against oxidant killing. | xanthomonas encounters highly toxic reactive oxygen species (ros) from many sources, such as those generated by plants against invading bacteria, other soil bacteria and from aerobic respiration. thus, conditions that alter intracellular ros levels such as exposure to toxic metalloids would have profound effects on bacterial physiology. here, we report that exposure of xanthomonas campestris pv. phaseoli (xp) to low levels of arsenic induces physiological cross-protection against killing by h(2) ... | 2006 | 16907748 |
| in vivo induced antigen technology (iviat) and change mediated antigen technology (cmat). | in this chapter, an overview of in vivo induced antigen technology (iviat) and change mediated antigen technology (cmat) will be presented, including a discussion of the advantages and limitations of these methods. over fifteen different microbial pathogens have been or are known to be currently studied with these methods. salient data obtained from the application of iviat and/or cmat to a selection of human and plant pathogens will be summarized. this includes recent reports on streptococcus p ... | 2006 | 16918490 |
| sumo-conjugating and sumo-deconjugating enzymes from arabidopsis. | posttranslational protein modification by the small ubiquitin-like modifier (sumo) is a highly dynamic and reversible process. to analyze the substrate specificity of sumo-conjugating and -deconjugating enzymes from arabidopsis (arabidopsis thaliana), we reconstituted its sumoylation cascade in vitro and tested the capacity of this system to conjugate the arabidopsis sumo isoforms atsumo1, 2, and 3 to the model substrate scpcna from yeast (saccharomyces cerevisiae). this protein contains two in ... | 2006 | 16920872 |
| pseudomonas syringae hrpj is a type iii secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the hrpz1 harpin. | the bacterial plant pathogen pseudomonas syringae requires a type iii protein secretion system (ttss) to cause disease. the p. syringae ttss is encoded by the hrp-hrc gene cluster. one of the genes within this cluster, hrpj, encodes a protein with weak similarity to yopn, a type iii secreted protein from the animal pathogenic yersinia species. here, we show that hrpj is secreted in culture and translocated into plant cells by the p. syringae pv. tomato dc3000 ttss. a dc3000 hrpj mutant, unl140, ... | 2006 | 16923873 |
| compensatory functions of two alkyl hydroperoxide reductases in the oxidative defense system of legionella pneumophila. | legionella pneumophila expresses two catalase-peroxidase enzymes that exhibit strong peroxidatic but weak catalatic activities, suggesting that other enzymes participate in decomposition of hydrogen peroxide (h2o2). comparative genomics revealed that l. pneumophila and its close relative coxiella burnetii each contain two peroxide-scavenging alkyl hydroperoxide reductase (ahpc) systems: ahpc1, which is similar to the helicobacter pylori ahpc system, and ahpc2 ahpd (ahpc2d), which is similar to t ... | 2006 | 16923890 |
| bacillus subtilis aconitase is required for efficient late-sporulation gene expression. | bacillus subtilis aconitase, encoded by the citb gene, is homologous to the bifunctional eukaryotic protein irp-1 (iron regulatory protein 1). like irp-1, b. subtilis aconitase is both an enzyme and an rna binding protein. in an attempt to separate the two activities of aconitase, the c-terminal region of the b. subtilis citb gene product was mutagenized. the resulting strain had high catalytic activity but was defective in sporulation. the defect was at a late stage of sporulation, specifically ... | 2006 | 16923907 |
| expression and functional roles of the pepper pathogen-induced transcription factor rav1 in bacterial disease resistance, and drought and salt stress tolerance. | a novel pathogen-induced gene encoding the rav (related to abi3/vp1) transcription factor, carav1, was isolated from pepper leaves infected with xanthomonas campestris pv. vesicatoria. carav1 contains two distinct dna-binding domains ap2 and b3 uniquely found in higher plants. transient expression analysis of the smgfp:carav1 fusion construct in arabidopsis protoplasts and pepper epidermal cells revealed the carav1 protein to be localized in the nucleus. the n-terminal region of carav1 fused to ... | 2006 | 16927203 |
| integrated regulation of the type iii secretion system and other virulence determinants in ralstonia solanacearum. | in many plant and animal bacterial pathogens, the type iii secretion system (ttss) that directly translocates effector proteins into the eukaryotic host cells is essential for the development of disease. in all species studied, the transcription of the ttss and most of its effector substrates is tightly regulated by a succession of consecutively activated regulators. however, the whole genetic programme driven by these regulatory cascades is still unknown, especially in bacterial plant pathogens ... | 2006 | 16933989 |
| hormonal and stress induction of the gene encoding common bean acetyl-coenzyme a carboxylase. | regulation of the cytosolic acetyl-coenzyme a carboxylase (accase) gene promoter from common bean (phaseolus vulgaris) was studied in transgenic arabidopsis (arabidopsis thaliana) plants using a beta-glucuronidase (gus) reporter gene fusion (pvaccase::gus). under normal growth conditions, gus was expressed in hydathodes, stipules, trichome bases, flowers, pollen, and embryos. in roots, expression was observed in the tip, elongation zone, hypocotyl-root transition zone, and lateral root primordia ... | 2006 | 16935989 |
| specific binding of the xanthomonas campestris pv. vesicatoria arac-type transcriptional activator hrpx to plant-inducible promoter boxes. | the pathogenicity of the plant-pathogenic bacterium xanthomonas campestris pv. vesicatoria depends on a type iii secretion system which is encoded by the 23-kb hrp (hypersensitive response and pathogenicity) gene cluster. expression of the hrp operons is strongly induced in planta and in a special minimal medium and depends on two regulatory proteins, hrpg and hrpx. in this study, dna affinity enrichment was used to demonstrate that the arac-type transcriptional activator hrpx binds to a conserv ... | 2006 | 16936021 |
| bacterial elicitation and evasion of plant innate immunity. | recent research on plant responses to bacterial attack has identified extracellular and intracellular host receptors that recognize conserved pathogen-associated molecular patterns and more specialized virulence proteins, respectively. these findings have shed light on our understanding of the molecular mechanisms by which bacteria elicit host defences and how pathogens have evolved to evade or suppress these defences. | 2006 | 16936700 |
| dual signaling functions of the hybrid sensor kinase rpfc of xanthomonas campestris involve either phosphorelay or receiver domain-protein interaction. | the hybrid sensor kinase rpfc positively regulates the expression of a range of virulent genes and negatively modulates the synthesis of the quorum sensing signal diffusible signal factor (dsf) in xanthomonas campestris. three conserved amino acid residues of rpfc implicated in phosphorelay (his(198) in the histidine kinase domain, asp(512) in the receiver domain, and his(657) in the histidine phosphotransfer domain) were essential for activation of the production of extracellular enzymes and ex ... | 2006 | 16940295 |
| crystallization and preliminary crystallographic characterization of gumk, a membrane-associated glucuronosyltransferase from xanthomonas campestris required for xanthan polysaccharide synthesis. | gumk is a membrane-associated inverting glucuronosyltransferase that is part of the biosynthetic route of xanthan, an industrially important exopolysaccharide produced by xanthomonas campestris. the enzyme catalyzes the fourth glycosylation step in the pentasaccharide-p-p-polyisoprenyl assembly, an oligosaccharide diphosphate lipid intermediate in xanthan biosynthesis. gumk has marginal homology to other glycosyltransferases (gts). it belongs to the cazy family gt 70, for which no structure is c ... | 2006 | 16946469 |
| crystal structure of the conserved hypothetical cytosolic protein xcc0516 from xanthomonas campestris reveals a novel quaternary structure assembled by five four-helix bundles. | 2006 | 16948161 | |
| structure of the dps-like protein from sulfolobus solfataricus reveals a bacterioferritin-like dimetal binding site within a dps-like dodecameric assembly. | the superfamily of ferritin-like proteins has recently expanded to include a phylogenetically distinct class of proteins termed dps-like (dpsl) proteins. despite their distinct genetic signatures, members of this subclass share considerable similarity to previously recognized dps proteins. like dps, these proteins are expressed in response to oxidative stress, form dodecameric cage-like particles, preferentially utilize h(2)o(2) in the controlled oxidation of fe(2+), and possess a short n-termin ... | 2006 | 16953567 |
| expression of the escherichia coli irga homolog adhesin is regulated by the ferric uptake regulation protein. | the irga homolog adhesin (iha) is an adherence-conferring outer membrane protein of escherichia coli associated with enterohemorrhagic and uropathogenic strains. here, we used primer extension analysis to identify iha promoters in o157:h7 and uropathogenic e. coli strains. transcriptional fusions demonstrated that iha transcription is repressed by iron. gel shifts using purified ferric uptake regulator protein (fur) demonstrated that repression involves a direct interaction between fur and the i ... | 2006 | 16954050 |
| synthesis of a beta-(1-->3)-d-rhamnotetraose by a one-pot, multiple radical fragmentation. | a naturally occurring beta-(1-->3)-d-rhamnotetraose has been constructed under conditions of sequential beta-selective mannosylation controlled by the 4,6-o-[1-cyano-2-(2-iodophenyl)-ethylidene] protecting group. the route is concise, proceeding through a late-stage radical deoxygenation that successfully uncovers all four deoxy subunits at once. | 2006 | 16956218 |
| an oxidoreductase is involved in cercosporin degradation by the bacterium xanthomonas campestris pv. zinniae. | the polyketide toxin cercosporin plays a key role in pathogenesis by fungal species of the genus cercospora. the bacterium xanthomonas campestris pv. zinniae is able to rapidly degrade this toxin. growth of x. campestris pv. zinniae strains in cercosporin-containing medium leads to the breakdown of cercosporin and to the formation of xanosporic acid, a nontoxic breakdown product. five non-cercosporin-degrading mutants of a strain that rapidly degrades cercosporin (xcz-3) were generated by ethyl ... | 2006 | 16957231 |
| the selective value of bacterial shape. | why do bacteria have shape? is morphology valuable or just a trivial secondary characteristic? why should bacteria have one shape instead of another? three broad considerations suggest that bacterial shapes are not accidental but are biologically important: cells adopt uniform morphologies from among a wide variety of possibilities, some cells modify their shape as conditions demand, and morphology can be tracked through evolutionary lineages. all of these imply that shape is a selectable featur ... | 2006 | 16959965 |
| complete nucleotide sequence of an exogenously isolated plasmid, plb1, involved in gamma-hexachlorocyclohexane degradation. | the alpha-proteobacterial strain sphingobium japonicum ut26 utilizes a highly chlorinated pesticide, gamma-hexachlorocyclohexane (gamma-hch), as a sole source of carbon and energy, and haloalkane dehalogenase linb catalyzes the second step of gamma-hch degradation in ut26. functional complementation of a linb mutant of ut26, ut26db, was performed by the exogenous plasmid isolation technique using hch-contaminated soil, leading to our successful identification of a plasmid, plb1, carrying the lin ... | 2006 | 16963556 |
| intricate knots in proteins: function and evolution. | our investigation of knotted structures in the protein data bank reveals the most complicated knot discovered to date. we suggest that the occurrence of this knot in a human ubiquitin hydrolase might be related to the role of the enzyme in protein degradation. while knots are usually preserved among homologues, we also identify an exception in a transcarbamylase. this allows us to exemplify the function of knots in proteins and to suggest how they may have been created. | 2006 | 16978047 |
| the tadv protein of actinobacillus actinomycetemcomitans is a novel aspartic acid prepilin peptidase required for maturation of the flp1 pilin and tade and tadf pseudopilins. | the tad locus of actinobacillus actinomycetemcomitans encodes genes for the biogenesis of flp pili, which allow the bacterium to adhere tenaciously to surfaces and form strong biofilms. although tad (tight adherence) loci are widespread among bacterial and archaeal species, very little is known about the functions of the individual components of the tad secretion apparatus. here we characterize the mechanism by which the pre-flp1 prepilin is processed to the mature pilus subunit. we demonstrate ... | 2006 | 16980493 |
| ospf and ospc1 are shigella flexneri type iii secretion system effectors that are required for postinvasion aspects of virulence. | shigella flexneri is the causative agent of dysentery, and its pathogenesis is mediated by a type iii secretion system (t3ss). s. flexneri secretes effector proteins into the eukaryotic cell via the t3ss, and these proteins usurp host cellular functions to the benefit of the bacteria. ospf and ospc1 are known to be secreted by s. flexneri, but their functions are unknown. we transformed s. flexneri with a plasmid that expresses a two-hemagglutinin tag (2ha) in frame with ospf or ospc1 and verifi ... | 2006 | 16988276 |
| disruption of n-acyl homoserine lactone-mediated cell signaling and iron acquisition in epiphytic bacteria by leaf surface compounds. | since n-acyl homoserine lactones (ahls) are key mediators of cell density-dependent regulation of traits involved in virulence and epiphytic fitness in gram-negative bacteria such as pseudomonas syringae, a variety of plant species were examined to determine their production of leaf surface compounds that could interact with these signaling systems. leaf washings of 17 of 52 plant species tested stimulated or inhibited ahl-dependent traits in at least one of the bacterial reporter strains used. ... | 2006 | 16997987 |
| the crystal structure of the e. coli stress protein ycif. | ycif is a protein that is up-regulated when bacteria experience stress conditions, and is highly conserved in a range of bacterial species. ycif has no known structure or biochemical function. to learn more about its potential molecular function and its role in the bacterial stress response, we solved the crystal structure of ycif at 2.0 angstrom resolution by the multiple wavelength anomalous diffraction (mad) technique. ycif is a dimer in solution, and forms a homodimer in the crystal asymmetr ... | 2006 | 17001035 |
| resistance of yersinia pestis to antimicrobial agents. | 2006 | 17005799 | |
| cell-cell signaling, cyclic di-gmp turnover and regulation of virulence in xanthomonas campestris. | the synthesis of virulence factors in the plant pathogen xanthomonas campestris pathovar campestris is regulated by cell-cell signaling mediated by a diffusible signal factor (dsf), and by the rpfc/rpfg two-component regulatory system. recent findings have indicated that the perception of the dsf signal requires the rpfc sensor and is linked to the degradation of the intracellular second messenger cyclic di-gmp by the hd-gyp domain regulator rpfg. | 2006 | 17008065 |
| cloning, crystallization and preliminary x-ray study of xc1258, a cn-hydrolase superfamily protein from xanthomonas campestris. | cn-hydrolase superfamily proteins are involved in a wide variety of non-peptide carbon-nitrogen hydrolysis reactions, producing some important natural products such as auxin, biotin, precursors of antibiotics etc. these reactions all involve attack on a cyano or carbonyl carbon by a conserved novel catalytic triad glu-lys-cys through a thiol acylenzyme intermediate. however, classification into the cn-hydrolase superfamily based on sequence similarity alone is not straightforward and further str ... | 2006 | 17012795 |
| the cloning, crystallization and preliminary x-ray analysis of xc2113, a yaeq protein from xanthomonas campestris. | xanthomonas campestris is a gram-negative bacterium that is phytopathogenic to cruciferous plants and causes worldwide agricultural loss. it is therefore important to identify potential pathogenic factors involved in this plant disease. here, the cloning, expression, crystallization and preliminary x-ray analysis of xc2113, a yaeq protein possibly involved in the production of virulence factors in xanthomonas campestris pathovar campestris, are reported. the xc2113 crystals diffracted well to a ... | 2006 | 17012809 |
| thermus thermophilus bacteriophage phiys40 genome and proteomic characterization of virions. | we determined the sequence of the 152,372 bp genome of phiys40, a lytic tailed bacteriophage of thermus thermophilus. the genome contains 170 putative open reading frames and three trna genes. functions for 25% of phiys40 gene products were predicted on the basis of similarity to proteins of known function from diverse phages and bacteria. phiys40 encodes a cluster of proteins involved in nucleotide salvage, such as flavin-dependent thymidylate synthase, thymidylate kinase, ribonucleotide reduct ... | 2006 | 17027029 |
| akinbetagamma contributes to snrk1 heterotrimeric complexes and interacts with two proteins implicated in plant pathogen resistance through its kis/gbd sequence. | the sucrose nonfermenting-1 protein kinase (snf1)/amp-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. in yeast (saccharomyces cerevisiae) and mammals, the beta- and gamma-noncatalytic subunits are implicated in substrate specificity and subcellular localization, respectively, and regulation of the kinase activity. the atypical betagamma-subunit has been previously described in maize (zea mays), presenting at its n-terminal ... | 2006 | 17028154 |
| cyclic di-gmp signaling in bacteria: recent advances and new puzzles. | 2006 | 17028282 | |
| crystal structure of xc5357 from xanthomonas campestris: a putative tetracenomycin polyketide synthesis protein adopting a novel cupin subfamily structure. | 2006 | 17029242 | |
| the crystal structure of xc847 from xanthomonas campestris: a 3'-5' oligoribonuclease of dnaq fold family with a novel opposingly shifted helix. | 2006 | 17029243 | |
| analysis of the dermatophyte trichophyton rubrum expressed sequence tags. | dermatophytes are the primary causative agent of dermatophytoses, a disease that affects billions of individuals worldwide. trichophyton rubrum is the most common of the superficial fungi. although t. rubrum is a recognized pathogen for humans, little is known about how its transcriptional pattern is related to development of the fungus and establishment of disease. it is therefore necessary to identify genes whose expression is relevant to growth, metabolism and virulence of t. rubrum. | 2006 | 17032460 |
| terminal reassortment drives the quantum evolution of type iii effectors in bacterial pathogens. | many bacterial pathogens employ a type iii secretion system to deliver type iii secreted effectors (t3ses) into host cells, where they interact directly with host substrates to modulate defense pathways and promote disease. this interaction creates intense selective pressures on these secreted effectors, necessitating rapid evolution to overcome host surveillance systems and defenses. using computational and evolutionary approaches, we have identified numerous mosaic and truncated t3ses among an ... | 2006 | 17040127 |
| kinase activity of overexpressed hipa is required for growth arrest and multidrug tolerance in escherichia coli. | overexpression of the hipa protein of the hipba toxin/antitoxin module leads to multidrug tolerance in escherichia coli. hipa is a "toxin" that causes reversible dormancy, whereas hipb is an antitoxin that binds hipa and acts as a transcriptional repressor of the hipba operon. comparative sequence analysis shows that hipa is a member of the phosphatidylinositol 3/4-kinase superfamily. the kinase activity of hipa was examined. hipa was autophosphorylated in the presence of atp in vitro, and the p ... | 2006 | 17041039 |
| molecular evolution of the h-ns protein: interaction with hha-like proteins is restricted to enterobacteriaceae. | we show here that chromosomal hha-like genes are restricted to the enterobacteriaceae. the h-ns n-terminal domain of members of this family includes an unaltered seven-amino-acid sequence located between helixes 1 and 2, termed the hha signature, that contains key residues for h-ns-hha interaction. | 2007 | 17041043 |
| molecular evolution of the h-ns protein: interaction with hha-like proteins is restricted to enterobacteriaceae. | we show here that chromosomal hha-like genes are restricted to the enterobacteriaceae. the h-ns n-terminal domain of members of this family includes an unaltered seven-amino-acid sequence located between helixes 1 and 2, termed the hha signature, that contains key residues for h-ns-hha interaction. | 2007 | 17041043 |
| evaluation of the antibacterial spectrum of drosocin analogues. | drosocin is a 19-mer, cationic antimicrobial peptide from drosophila melanogaster. the aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. furthermore, the amino acid sequence of dnak, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. from this a panel of 31 bacterial strains, including salmonella strains with truncated lipopolysaccharide structures, was tested for susceptibility towards three dro ... | 2006 | 17062012 |
| towards a conceptual and operational union of bacterial systematics, ecology, and evolution. | to completely understand the ecology of a bacterial community, we need to identify its ecologically distinct populations (ecotypes). the greatest promise for enumerating a community's constituent ecotypes is held by molecular approaches that identify bacterial ecotypes as dna sequence clusters. these approaches succeed when ecotypes correspond with sequence clusters, but some models of bacterial speciation predict a one-to-many and others a many-to-one relationship between ecotypes and sequence ... | 2006 | 17062416 |
| an inventory of yeast proteins associated with nucleolar and ribosomal components. | although baker's yeast is a primary model organism for research on eukaryotic ribosome assembly and nucleoli, the list of its proteins that are functionally associated with nucleoli or ribosomes is still incomplete. we trained a naïve bayesian classifier to predict novel proteins that are associated with yeast nucleoli or ribosomes based on parts lists of nucleoli in model organisms and large-scale protein interaction data sets. phylogenetic profiling and gene expression analysis were carried ou ... | 2006 | 17067374 |
| cloning, crystallization and preliminary x-ray studies of xc2981 from xanthomonas campestris, a putative cuta1 protein involved in copper-ion homeostasis. | divalent metal ions play key roles in all living organisms, serving as cofactors for many proteins involved in a variety of electron-transfer activities. however, copper ions are highly toxic when an excessive amount is accumulated in a cell. cuta1 is a protein found in all kingdoms of life that is believed to participate in copper-ion tolerance in escherichia coli, although its specific function remains unknown. several crystal structures of multimeric cuta1 with different rotation angles and d ... | 2006 | 17077491 |
| salmonella produces an o-antigen capsule regulated by agfd and important for environmental persistence. | in this study, we show that salmonella produces an o-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. structural analysis of purified salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of salmonella enterica serovar enteritidis lipopolysaccharide o antigen with some modifications. putative carbohydrate transport and regulatory operons important for capsule assembly and translocation, designat ... | 2006 | 17079680 |
| from the academy: colloquium review. unique characteristics of xanthomonas oryzae pv. oryzae avrxa21 and implications for plant innate immunity. | this article provides a brief overview of some of the major concepts and molecular features of plant and animal innate immune systems. the rice pathogen recognition receptor, xa21, confers resistance to xanthomonas oryzae pv. oryzae strains producing the avrxa21 elicitor. xa21 codes for a receptor-like kinase consisting of an extracellular leucine-rich repeat domain, a transmembrane domain, and a cytoplasmic kinase domain. we show that avrxa21 activity requires the presence of rax (required for ... | 2006 | 17082309 |
| transcriptome changes in the phenylpropanoid pathway of glycine max in response to pseudomonas syringae infection. | reports of plant molecular responses to pathogenic infections have pinpointed increases in activity of several genes of the phenylpropanoid pathway leading to the synthesis of lignin and flavonoids. the majority of those findings were derived from single gene studies and more recently from several global gene expression analyses. we undertook a global transcriptional analysis focused on the response of genes of the multiple branches of the phenylpropanoid pathway to infection by the pseudomonas ... | 2006 | 17083738 |
| genomic structure and phylogeny of the plant pathogen ralstonia solanacearum inferred from gene distribution analysis. | in the present study, we investigated the gene distribution among strains of the highly polymorphic plant pathogenic beta-proteobacterium ralstonia solanacearum, paying particular attention to the status of known or candidate pathogenicity genes. based on the use of comparative genomic hybridization on a pangenomic microarray for the gmi1000 reference strain, we have defined the conditions that allowed comparison of the repertoires of genes among a collection of 18 strains that are representativ ... | 2007 | 17085551 |
| genomic structure and phylogeny of the plant pathogen ralstonia solanacearum inferred from gene distribution analysis. | in the present study, we investigated the gene distribution among strains of the highly polymorphic plant pathogenic beta-proteobacterium ralstonia solanacearum, paying particular attention to the status of known or candidate pathogenicity genes. based on the use of comparative genomic hybridization on a pangenomic microarray for the gmi1000 reference strain, we have defined the conditions that allowed comparison of the repertoires of genes among a collection of 18 strains that are representativ ... | 2007 | 17085551 |
| bacillus subtilis gene cluster involved in calcium carbonate biomineralization. | calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. nevertheless, little is known of the molecular mechanisms by which bacteria foster calcium carbonate mineralization. in our laboratory, we are studying calcite formation by bacillus subtilis, in order to identify genes involved in the biomineralization process. a previous screening of uv mutants and of more than one thousand mutants ob ... | 2007 | 17085570 |