Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| autocatalytic rna cleavage in the human beta-globin pre-mrna promotes transcription termination. | new evidence indicates that termination of transcription is an important regulatory step, closely related to transcriptional interference and even transcriptional initiation. however, how this occurs is poorly understood. recently, in vivo analysis of transcriptional termination for the human beta-globin gene revealed a new phenomenon--co-transcriptional cleavage (cotc). this primary cleavage event within beta-globin pre-messenger rna, downstream of the poly(a) site, is critical for efficient tr ... | 2004 | 15565159 |
| analyses of expression and localization of two mammalian-type transglutaminases in physarum polycephalum, an acellular slime mold. | transglutaminase (tgase) is an enzyme that modifies proteins by crosslinking or polyamination. physarum polycephalum, an acellular slime mold, is the evolutionally lowest organism that has a mammalian-type transglutaminase. we have cloned a cdna for physarum polycephalum tgase (pptgb), homologous to a previously identified tgase (pptga), whose sequence is similar to that of mammalian tgases. pptgb encodes a primary sequence identical to that of pptga except for 11 amino acid residues at the n-te ... | 2004 | 15632307 |
| estimating the number of plasmids taken up by a eukaryotic cell during transfection and evidence that antisense rna abolishes gene expression in physarum polycephalum. | we have estimated the statistical distribution of the number of plasmids taken up by individual jurkat lymphoma cells during electroporation in the presence of two plasmids, one encoding for yellow (eyfp) the other for cyan (ecfp) fluorescent protein. the plasmid concentration at which most of the cells take up only one plasmid or several molecules was determined by statistical analysis. we found that cells behaved slightly heterogeneous in plasmid uptake and describe how the homogeneity of a ce ... | 2005 | 15667997 |
| rearrangements in the physarum polycephalum mitochondrial genome associated with a transition from linear mf-mtdna recombinants to circular molecules. | although mitochondrial dna (mtdna) is transmitted to progeny from one parent only in physarum polycephalum, the mtdnas of progeny of mf+ plasmodia vary in structure. to clarify the mechanisms associated with the mitochondrial plasmid mf that generate mtdna polymorphisms, 91 progeny of four strains (km88 x je8, km88 x tu111, km88 x ng111, je90) were investigated using rflp analysis, pcr, and pulse-field gel electrophoresis (pfge). nine mtdna rearrangement types were found, with rearrangements occ ... | 2004 | 15688251 |
| a calmodulin-dependent protein kinase from lower eukaryote physarum polycephalum. | a full-length cdna coding a calmodulin (cam)-dependent protein kinase gene was cloned from physarum plasmodia poly(a)-rna by polymerase chain reaction with the oligonucleotide primers that were designed after the amino acid sequence of highly conserved regions of myosin light-chain kinase. sequence analysis of the cdna revealed that this physarum kinase was a 42,519-da protein with an atp-binding domain, ser/thr kinase active site signature, and cam-binding domain. expression of the cdna in esch ... | 2005 | 15707955 |
| low-molecular-weight poly(alpha-methyl beta,l-malate) of microbial origin: synthesis and crystallization. | low-molecular-weight poly(alpha-methyl beta,l-malate) made of approximately 25-30 units was prepared from microbial poly(beta,l-malic acid) by treatment with diazomethane. the thermal characterization of the polymalate methyl ester was carried out and its crystalline structure was preliminary examined. its ability to crystallize both from solution and from the melt was comparatively evaluated. | 2005 | 15719432 |
| nonmuscle caldesmon: its distribution and involvement in various cellular processes. review article. | smooth muscle caldesmon is a thin-filament constituent which takes part in the ca2+-dependent regulation of actomyosin motor activity which converts chemical energy of atp into force. the molecular anatomy of its counterpart found in a variety of nonmuscle cells is similar. both contain about 20 nm long terminal domains responsible for functionally important multisite interactions with filamentous actin, tropomyosin, ca2+/calmodulin, and myosin and differ by a 35 nm long central, alpha-helical f ... | 2004 | 15726805 |
| replication-independent core histone dynamics at transcriptionally active loci in vivo. | we used a novel labeling technique in the naturally synchronous organism physarum polycephalum to examine the fate of core histones in g2 phase. we find rapid exchange of h2a/h2b dimers with free pools that is greatly diminished by treatment of the cells with alpha-amanitin. this exchange is enhanced in pol ii-coding sequences compared with extragenic regions or inactive loci. in contrast, h3/h4 tetramers exhibit far lower levels of exchange in the pol ii-transcribed genes tested, suggesting tha ... | 2005 | 15769942 |
| structural implications of novel diversity in eucaryal rnase p rna. | previous eucaryotic rnase p rna secondary structural models have been based on limited diversity, representing only two of the approximately 30 phylogenetic kingdoms of the domain eucarya. to elucidate a more generally applicable structure, we used biochemical, bioinformatic, and molecular approaches to obtain rnase p rna sequences from diverse organisms including representatives of six additional kingdoms of eucaryotes. novel sequences were from acanthamoeba (acathamoeba castellanii, balamuthia ... | 2005 | 15811915 |
| class-specific binding of two aminoacyl-trna synthetases to annexin, a ca2+- and phospholipid-binding protein. | annexins are a family of ca2+/phospholipid-binding proteins that have diverse functions. to understand the function of annexin in physarum polycephalum, we searched for its binding proteins. here we demonstrate the presence of two novel annexin-binding proteins. the homology search of partial amino acid sequences of these two proteins identified them as aminoacyl-trna synthetases (arss). furthermore, antibody against aminoacyl-trna synthetases cross-reacted with one of two proteins. our results ... | 2005 | 15840947 |
| induction of apoptosis by apicularen a in human promyelocytic leukemia cell line hl-60. | apicularen a, a macrolide isolated from the myxobacterial genus chondromyces, suppressed the proliferation of human promyelocytic leukemia cells (hl-60 cells), increased the release of lactate dehydrogenase and induced condensation and fragmentation of chromatin at 1 to 100 nm. in addition, it induced the dna fragmentation, increased the percentage of annexin v-stained cells, and cleaved poly(adp-ribose) polymerase (parp), a substrate of caspase. in contrast, apicularen b, an n-acetylglucosamine ... | 2005 | 15856405 |
| reconstructing the regulatory network controlling commitment and sporulation in physarum polycephalum based on hierarchical petri net modelling and simulation. | we reconstruct the regulatory network controlling commitment and sporulation of physarum polycephalum from experimental results using a hierarchical petri net-based modelling and simulation framework. the stochastic petri net consistently describes the structure and simulates the dynamics of the molecular network as analysed by genetic, biochemical and physiological experiments within a single coherent model. the petri net then is extended to simulate time-resolved somatic complementation experi ... | 2005 | 15904935 |
| use of the giant multinucleate plasmodium of physarum polycephalum to study rna interference in the myxomycete. | the plasmodium of physarum polycephalum harbors billions of synchronized nuclei in a single cell of complex structure. due to its synchrony and extreme size, it is used as a model to study events on a single cell level, such as cell cycle and differentiation. we show here for the first time that this model, despite its enormous size and structural complexity, is accessible to rna interference by simple injection of dsrna or sirna. the targeted gene is that of polymalatase, an intracellular adapt ... | 2005 | 15922285 |
| [isolation and characterization of the alp1 protease from aspergillus fumigatus and its protein inhibitor from physarium polycephalum]. | it is known that aspergillus fumigatus secretes a serine protease alp1 of the subtilisin family in the presence of extracellular protein substrates. we found conditions of a. fumigatus culturing that provide a high alp1 activity inside cells without induction by extracellular proteins. the identity of the properties of the secreted and intracellular enzymes was shown. a thermostable protein inhibitor of the alp1 protease was isolated from the plasmodium of the myxomycete physarum polycephalum. i ... | 2005 | 16004384 |
| archazolid and apicularen: novel specific v-atpase inhibitors. | v-atpases constitute a ubiquitous family of heteromultimeric, proton translocating proteins. according to their localization in a multitude of eukaryotic membranes, they energize many different transport processes. since their malfunction is correlated with various diseases in humans, the elucidation of the properties of this enzyme for the development of selective inhibitors and drugs is one of the challenges in v-atpase research. | 2005 | 16080788 |
| discovery of new genes and deletion editing in physarum mitochondria enabled by a novel algorithm for finding edited mrnas. | gene finding is complicated in organisms that exhibit insertional rna editing. here, we demonstrate how our new algorithm predictor of insertional editing (pie) can be used to locate genes whose mrnas are subjected to multiple frameshifting events, and extend the algorithm to include probabilistic predictions for sites of nucleotide insertion; this feature is particularly useful when designing primers for sequencing edited rnas. applying this algorithm, we successfully identified the nad2, nad4l ... | 2005 | 16147990 |
| model for codon position bias in rna editing. | rna editing can be crucial for the expression of genetic information via inserting, deleting, or substituting a few nucleotides at specific positions in an rna sequence. within coding regions in an rna sequence, editing usually occurs with a certain bias in choosing the positions of the editing sites. in the mitochondrial genes of physarum polycephalum, many more editing events have been observed at the third codon position than at the first and second, while in some plant mitochondria the secon ... | 2005 | 16196905 |
| [synchronization of mechanochemical auto-oscillations within the physarum polycephalum plasmodium by periodical external actions]. | amoeboid locomotion of huge unicellular organism, the physarum polycephalum plasmodium, is stipulated by endoplasmic flow, which is produced by spatially highly coordinated rhythmic contractions of the ectoplasm. to describe the self-organization of the plasmodial contractile activity, we proposed a mathematical model, which is based on the hypothesis of positive feedback between the deformation of the cytoskeleton and release of a chemical regulator of the active contraction. a nonautonomous an ... | 2005 | 16212064 |
| early zygote-specific nuclease in mitochondria of the true slime mold physarum polycephalum. | the active, selective digestion of mtdna from one parent is a possible molecular mechanism for the uniparental inheritance of mtdna. in physarum polycephalum, mtdna is packed by dna-binding protein glom, which packs mtdna into rod-shaped mt-nucleoids. after the mating, mtdna from one parent is selectively digested, and the glom began to disperse. dispersed glom was retained for at least 6 h after mtdna digestion, but disappeared completely by about 12 h after mixing two strains. we identified tw ... | 2005 | 16215759 |
| bacteria associated with spores of the arbuscular mycorrhizal fungi glomus geosporum and glomus constrictum. | spores of the arbuscular mycorrhizal fungi (amf) glomus geosporum and glomus constrictum were harvested from single-spore-derived pot cultures with either plantago lanceolata or hieracium pilosella as host plants. pcr-denaturing gradient gel electrophoresis analysis revealed that the bacterial communities associated with the spores depended more on amf than host plant identity. the composition of the bacterial populations linked to the spores could be predominantly influenced by a specific spore ... | 2005 | 16269696 |
| function of c-fos-like and c-jun-like proteins on trichostatin a-induced g2/m arrest in physarum polycephalum. | the homologs of transcription factors c-fos and c-jun have been detected in slime mold physarum polycephalum during progression of the synchronous cell cycle. here we demonstrated that c-fos-like and c-jun-like proteins participated in g2/m transition by the regulation of the level of cyclin b1 protein in p. polycephalum. the study of antibody neutralization revealed that interruption of the functions of c-fos-like and c-jun-like proteins resulted in g2/m transition arrest, implicating their fun ... | 2005 | 16270156 |
| myxobacters from arid mexican soil. | myxobacters were found to be common inhabitants of the arid soils from the monterrey, nuevo leon, mexico, area. thirteen species of the genera myxococcus, archangium, cystobacter, stigmatella, polyangium, and chondromyces were isolated on a mineral salts agar supplemented with bakers' yeast and filter paper. greater species diversity per soil sample was found in the region receiving 400 to 800 mm of annual rainfall as compared with soils from an area having only 200 to 400 mm of rainfall. | 1976 | 16345178 |
| regulation of levels of actin threonine phosphorylation during life cycle of physarum polycephalum. | under various environmental stresses, the true slime mold physarum polycephalum converts into dormant forms, such as microcysts, sclerotia, and spores, which can survive in adverse environments for a considerable period of time. in drought-induced sclerotia, actin is threonine phosphorylated, which blocks its ability to polymerize into filaments. it is known that fragmin and actin-fragmin kinase (afk) mediate this phosphorylation event. in this work, we demonstrate that high levels of actin thre ... | 2006 | 16374832 |
| identification of a putative mitochondrial rna polymerase from physarum polycephalum: characterization, expression, purification, and transcription in vitro. | mitochondrial rna polymerases (mtrnaps) are necessary for the biogenesis of mitochondria and for proper mitochondrial function since they transcribe genes on mtdna for trnas, rrnas, and mrnas. the unique type of rna editing identified in mitochondria of physarum polycephalum is thought to be closely associated with transcription, and as such, rna editing activity would be expected to be closely associated with the mtrnap. in order to better characterize the role of mtrnaps in mitochondrial bioge ... | 2006 | 16402203 |
| histone h4 gene is transcribed in s phase but also late in g(2) phase in physarum polycephalum. | the myxomycete physarum polycephalum contains two types of h4 histone genes. southern blotting of restriction endonuclease fragments of p. polycephalum dna and hybridization to a cloned probe labelled by nick-translation indicate that there are only one or two copies of each h4 gene per haploid genome. a cloned homologous genomic probe was used to study the cellular abundance of h4 mrna during the cell cycle. we report that the h4 mrna is not only transcribed in s phase as previously described f ... | 1984 | 16453570 |
| transposon-like properties of the major, long repetitive sequence family in the genome of physarum polycephalum. | a family of long, highly-repetitive sequences, referred to previously as ;hpaii-repeats', dominates the genome of the eukaryotic slime mould physarum polycephalum. these sequences are found exclusively in scrambled clusters. they account for about one-half of the total complement of repetitive dna in physarum, and represent the major sequence component found in hypermethylated, 20-50 kb segments of physarum genomic dna that fail to be cleaved using the restriction endonuclease hpaii. the structu ... | 1985 | 16453652 |
| screening for beta-poly(l-malate) binding proteins by affinity chromatography. | poly(beta-l-malic acid) is a cell type-specific polymer of myxomycetes (true slime molds) with the physiological role to organize mobility of certain proteins over the giant multinucleated plasmodia. we have developed an affinity chromatography employing 1,6-diamino-n-hexane-sepharose-coupled poly(malic acid) to identify such proteins in cellular extracts of physarum polycephalum. molecular masses were measured by sds-page and non-denaturing page after silver staining and/or western blotting. pr ... | 2006 | 16476581 |
| stage specific expression of poly(malic acid)-affiliated genes in the life cycle of physarum polycephalum. spherulin 3b and polymalatase. | polymalic acid is receiving interest as a unique biopolymer of the plasmodia of mycetozoa and recently as a biogenic matrix for the synthesis of devices for drug delivery. the acellular slime mold physarum polycephalum is characterized by two distinctive growth phases: uninucleated amoebae and multinucleated plasmodia. in adverse conditions, plasmodia reversibly transform into spherules. only plasmodia synthesize poly(malic acid) (pmla) and pmla-hydrolase (polymalatase). we have performed suppre ... | 2006 | 16478477 |
| isolation and characterization of an extracellular polysaccharide from physarum polycephalum. | the myxomycetes are called slime molds because of the synthesis of copious amounts of extracellular material (slime) during parts of the life cycle. in physarum polycephalum, small amounts of slime are produced during exponential growth of microplasmodia in shake flasks, but the amount of this slime increased 10- to 20-fold at 16 to 34 hr after microplasmodia were induced to form spherules by transferring them to salt solution. the slime obtained during both periods is the same; an acidic polysa ... | 1970 | 16559083 |
| isolation and characterization of a galactosamine wall from spores and spherules of physarum polycephalum. | the myxomycete, physarum polycephalum, can be induced under laboratory conditions to form two different hard-walled forms, spores and spherules. characterization of both types of walls revealed only a single sugar, galactosamine. it was identified after acid hydrolysis of the isolated walls by chromatography in three solvent systems, by its positive reaction with ammoniacal silver nitrate, ninhydrin, galactostat, and the elson-morgan test, and by ninhydrin degradation to lyxose. galactosamine wa ... | 1970 | 16559084 |
| the enzymatic oxidation of ascorbic acid in the slime mold, physarum polycephalum. | 1955 | 16654729 | |
| structure, function and evolution of the mitochondrial division apparatus. | mitochondria are derived from free-living alpha-proteobacteria that were engulfed by eukaryotic host cells through the process of endosymbiosis, and therefore have their own dna which is organized using basic proteins to form organelle nuclei (nucleoids). mitochondria divide and are split amongst the daughter cells during cell proliferation. their division can be separated into two main events: division of the mitochondrial nuclei and division of the matrix (the so-called mitochondrial division, ... | 2006 | 16690143 |
| mathematical model for rhythmic protoplasmic movement in the true slime mold. | the plasmodium of the true slime mold physarum polycephalum is a large amoeboid organism that displays "smart" behavior such as chemotaxis and the ability to solve mazes and geometrical puzzles. these amoeboid behaviors are based on the dynamics of the viscoelastic protoplasm and its biochemical rhythms. by incorporating both these aspects, we constructed a mathematical model for the dynamics of the organism as a first step towards understanding the relation between protoplasmic movement and its ... | 2006 | 16770610 |
| molecular and biochemical studies of chondramide formation-highly cytotoxic natural products from chondromyces crocatus cm c5. | the jaspamide/chondramide family of depsipeptides are mixed pks/nrps natural products isolated from marine sponges and a terrestrial myxobacterium that potently affect the function of the actin cytoskeleton. as a first step to improve production in heterologous host cells and permit genetic approaches to novel analogs, we have cloned and characterized the chondramide biosynthetic genes from the myxobacterium chondromyces crocatus cm c5. in addition to the expected pks and nrps genes, the cluster ... | 2006 | 16793524 |
| migration in dictyostelium polycephalum. | by comparing two species of cellular slime molds that have stalkless migration stages it is possible to gain interesting insights into how the cells move. in contrast to the familiar behavior of dictyostelium discoideum, dictyostelium polycephalum slugs can travel greater distances through soil and even can migrate through agar. in addition to the interest in the differences, these differences shed light on the mechanism of slug movement. unlike d. discoideum, d. polycephalum does not have prest ... | 2006 | 16894971 |
| a cdna cloned from physarum polycephalum encodes new type of family 3 beta-glucosidase that is a fusion protein containing a calx-beta motif. | the microplasmodia of physarum polycephalum express three types of beta-glucosidases: secretory enzyme, a soluble cytoplasmic enzyme and a membrane-bound enzyme. we are interested in the physiological role of three enzymes. we report the sequence of cdna for membrane beta-glucosidase 1, which consists of 3825 nucleotides that includes an open reading frame encoding 1248 amino acids. the molecular weight of membrane beta-glucosidase 1 was calculated to be 131,843 based on the predicted amino acid ... | 2006 | 16914364 |
| membrane biophysics of chemoreception and taxis in the plasmodium of physarum polycephalum. | the threshold phenomena observed in chemoreception and taxis of the plasmodium of physarum polycephalum were analyzed on the basis of physical chemistry. various physicochemical concepts and rules, e.g. the schulze-hardy rule, the lyotropic number and the hydrophobic interactions, were shown to be applicable reasonably well to the physiological functions in physarum. it was stressed that the structural change of the surface membrane induced by reception of chemical stimuli plays a decisive role ... | 1980 | 16997266 |
| progress in plasmodial differentiation improves regularity of oscillating contractions in physarum polycephalum. | based on the knowledge about subcellular morphogenetic processes in the acellular slime mold physarum polycephalum, we hypothesized that during differentiation of undifferentiated endoplasm to the highly differentiated complex structure of the contractile apparatus of this organism, the regularity of oscillating contractions must improve. we measured the endogenous contraction automaticity starting from the de novo generation within minutes after sampling small portions of undifferentiated endop ... | 2007 | 17045817 |
| a mathematical model for adaptive transport network in path finding by true slime mold. | we describe here a mathematical model of the adaptive dynamics of a transport network of the true slime mold physarum polycephalum, an amoeboid organism that exhibits path-finding behavior in a maze. this organism possesses a network of tubular elements, by means of which nutrients and signals circulate through the plasmodium. when the organism is put in a maze, the network changes its shape to connect two exits by the shortest path. this process of path-finding is attributed to an underlying ph ... | 2007 | 17069858 |
| inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis. | we have previously shown that laminin-8, a vascular basement membrane component, was overexpressed in human glioblastomas multiforme and their adjacent tissues compared to normal brain. increased laminin-8 correlated with shorter glioblastoma recurrence time and poor patient survival making it a potential marker for glioblastoma diagnostics and prediction of disease outcome. however, laminin-8 therapeutic potential was unknown because the technology of blocking the expression of multi-chain comp ... | 2006 | 17109197 |
| [thermostable extracellular cyclic nucleotide phosphodiesterase from physarum polycephalum plasmodium]. | the cyclic nucleotide phosphodiesterase secreted by physarum polycephalum plasmodium into extracellular medium has been partially purified by deae cellulose chromatography, ultrafiltration, and hplc. the results obtained by gel filtration, hplc, electrophoresis, and isoelectric focusing suggest that, the native enzyme in solution is a monomer with a molecular mass of about 90 kda and pi in the range 3.6 - 4.0. the km values were estimated to be about 0.9 mm and 7.7 mm, respectively, and vm for b ... | 2006 | 17131817 |
| [chemotaxis as a method for testing of the biological effects of silver nanoparticles]. | a method for assessing the abiotic efficiency of water-dispersed nano-sized silver particles is suggested. nanoparticles were obtained by the method of biochemical synthesis in reversed micelles made from anionic surfactant bis-(2-ethylhexyl) sodium sulfosuccinate (aot). a water dispersion of nanoparticles was prepared from the micellar solution in organic solvent by means of a special procedure. the abiotic efficiency assay is based on the capacity of cells to respond by the negative chemotaxis ... | 2006 | 17131825 |
| molecular and biochemical characterization of a novel actin bundling protein in acanthamoeba. | actin binding proteins play key roles in cell structure and movement particularly as regulators of the assembly, stability and localization of actin filaments in the cytoplasm. in the present study, a cdna clone encoding an actin bundling protein named as ahabp was isolated from acanthamoeba healyi, a causative agent of granulomatous amebic encephalitis. this clone exhibited high similarity with genes of physarum polycephalum and dictyostelium discoideum, which encode actin bundling proteins. do ... | 2006 | 17170575 |
| robot control with biological cells. | at present there exists a large gap in size, performance, adaptability and robustness between natural and artificial information processors for performing coherent perception-action tasks under real-time constraints. even the simplest organisms have an enviable capability of coping with an unknown dynamic environment. robots, in contrast, are still clumsy if confronted with such complexity. this paper presents a bio-hybrid architecture developed for exploring an alternate approach to the control ... | 2007 | 17188804 |
| a 368-base-pair cis-acting hwp1 promoter region, hcr, of candida albicans confers hypha-specific gene regulation and binds architectural transcription factors nhp6 and gcf1p. | to elucidate the molecular mechanisms controlling the expression of the hypha-specific adhesin gene hwp1 of candida albicans, its promoter was dissected and analyzed using a green fluorescent protein reporter gene. a 368-bp region, the hwp1 control region (hcr), was critical for activation under hypha-inducing conditions and conferred developmental regulation to a heterologous eno1 promoter. a more distal region of the promoter served to amplify the level of promoter activation. using gel mobili ... | 2007 | 17220463 |
| dispersion relation in oscillatory reaction-diffusion systems with self-consistent flow in true slime mold. | in the large amoeboid organism physarum, biochemical oscillators are spatially distributed throughout the organism and their collective motion exhibits phase waves, which carry physiological signals. the basic nature of this wave behaviour is not well-understood because, to date, an important effect has been neglected, namely, the shuttle streaming of protoplasm which accompanies the biochemical rhythms. here we study the effects of self-consistent flow on the wave behaviour of oscillatory react ... | 2007 | 17235581 |
| a gene, alca, affecting the life cycle form expressed in physarum polycephalum. | the usual sequence of forms in the physarum polycephalum life cycle is plasmodium-spore-amoeba-plasmodium. so-called "amoebaless life cycle" or alc mutants of this myxomycete undergo a simplified plasmodium-spore-plasmodium life cycle. we have analyzed three independently isolated alc mutants and found in each case that the failure of the spores to give rise to amoebae is due to a recessive mendelian allele. the three mutations are tightly linked to one another and belong to a single complementa ... | 1982 | 17246081 |
| genetics of somatic fusion in physarum polycephalum: the ppii strain. | plasmodial (somatic) fusion in a strain of physarum polycephalum, a true slime mold, is controlled by four loci, each of which displays simple dominance. two diploid plasmodia fuse with each other only if they are phenotypically or genotypically identical for all four fusion loci. | 1972 | 17248576 |
| mutations increasing asexual plasmodium formation in physarum polycephalum. | rare plasmodia formed in clones of heterothallic amoebae were analyzed in a search for mutations affecting plasmodium formation. the results show that the proportion of mutants varies with both temperature (18 degrees , 26 degrees or 30 degrees ) and mating-type allele (mt1, mt2, mt3, mt4). at one extreme, only one of 33 plasmoida formed by mt2 amoebae at 18 degrees is mutant. at the other extreme, three of three plasmodia formed by mt1 amoebae at 30 degrees are mutant. the mutant plasmodia fall ... | 1977 | 17248771 |
| complementation of amoebal-plasmodial transition mutants in physarum polycephalum. | amoebae of the myxomycete physarum polycephalum differentiate to yield plasmodia in two ways: in crossing, haploid amoebae of appropriate genotypes fuse to form diploid plasmodia; in selfing, plasmodia form without amoebal fusion or increase in ploidy. amoebae carrying the mating-type allele matah (formerly mt(h)) self efficiently, but occasionally give rise to mutants that self at very low frequencies. such "amoebal-plasmodial transition" mutants were mixed in pairs to test their ability to com ... | 1979 | 17248891 |
| a new mating compatibility locus in physarum polycephalum. | the rate and extent of plasmodium formation were studied in mating tests involving pairs of largely isogenic amoebal strains compatible for mating-type (mt) alleles. a systematic variability was observed: plasmodia formed either rapidly and extensively or slowly and inefficiently. plasmodium formation was found to be 10(3)- to 10(4)-fold more extensive in "rapid" crosses than in "slow" crosses. a genetic analysis revealed that the variability reflects the influence of a multiallelic compatibilit ... | 1979 | 17248906 |
| two multiallelic mating compatibility loci separately regulate zygote formation and zygote differentiation in the myxomycete physarum polycephalum. | the mating of physarum polycephalum amoebae, the ultimate consequence of which is a "plasmodium," was recently shown to be governed by two compatibility loci, mata (or mt) and matb (dee 1978; youngmanet al. 1979). we present evidence that mata and matb separately regulate two discrete stages of mating: in the first stage, amoebae (which are normally haploid) fuse in pairs, with a specificity determined by matb genotype, to form diploid zygotes; subsequent differentiation of the zygotes into plas ... | 1981 | 17249081 |
| structure and properties of the recombinant nadh-cytochrome b5 reductase of physarum polycephalum. | a cdna for nadh-cytochrome b(5) reductase of physarum polycephalum was cloned from a cdna library, and the nucleotide sequence of the cdna was determined (accession no. ab259870). the dna of 943 base pairs contains 5'- and 3'-noncoding sequences, including a polyadenylation sequence, and a coding sequence of 843 base pairs. the amino acid sequence (281 residues) deduced from the nucleotide sequence was 25 residues shorter than those of vertebrate enzymes. nevertheless, the recombinant physarum e ... | 2007 | 17341833 |
| clathrin is involved in organization of mitotic spindle and phragmoplast as well as in endocytosis in tobacco cell cultures. | we previously identified a 175 kda polypeptide in lilium longiflorum germinating pollen using a monoclonal antibody raised against myosin ii heavy chain from physarum polycephalum. in the present study, the equivalent polypeptide was also found in cultured tobacco by-2 cells. analysis of the amino acid sequences revealed that the 175 kda polypeptide is clathrin heavy chain and not myosin heavy chain. after staining of by-2 cells, punctate clathrin signals were distributed throughout the cytoplas ... | 2007 | 17351731 |
| [cell biology researches aboard the robotic space vehicles: preparation and performance]. | the article reviews the unique aspects of preparation and performance of cell biology experiments flown on robotic space vehicles bion and foton, and gives an overview of key findings in researches made under the author's leadership over the past decades. described are the criteria of selecting test objects, and the conditions required for preparation and implementation of space and control (synchronous) experiments. the present-day status and issues of researches into cell responsivity to space ... | 2006 | 17357620 |
| nanoconjugate based on polymalic acid for tumor targeting. | a new prototype of polymer-derived drug delivery system, the nanoconjugate polycefin, was tested for its ability to accumulate in tumors based on enhanced permeability and retention (epr) effect and receptor mediated endocytosis. polycefin was synthesized for targeted delivery of morpholino antisense oligonucleotides into certain tumors. it consists of units that are covalently conjugated with poly(beta-l-malic acid) (m(w) 50,000, m(w)/m(n) 1.3) highly purified from cultures of myxomycete physar ... | 2008 | 17376417 |
| structure of physarum polycephalum cytochrome b5 reductase at 1.56 a resolution. | physarum polycephalum cytochrome b(5) reductase catalyzes the reduction of cytochrome b(5) by nadh. the structure of p. polycephalum cytochrome b(5) reductase was determined at a resolution of 1.56 a. the molecular structure was compared with that of human cytochrome b(5) reductase, which had previously been determined at 1.75 a resolution [bando et al. (2004), acta cryst. d60, 1929-1934]. the high-resolution structure revealed conformational differences between the two enzymes in the adenosine ... | 2007 | 17401193 |
| inhibition of bone resorption in cultures of mouse calvariae by apicularen a. | apicularens a and b were isolated from the myxobacterial genus chondromyces apiculatus jw184. apicularen a inhibited bafilomycin a1-sensitive atp-dependent proton transport into microsome vesicles more potently than apicularen b. bone resorption in cultures of mouse calvariae induced by human parathyroid hormone (pth) or interleukin-1beta (il-1beta) was inhibited by apicularen a at 10 and 100 nm, while apicularen b had no effect. the bisphosphonate incadronate inhibited bone resorption at 100 nm ... | 2007 | 17415879 |
| fourier-based spatial mapping of oscillatory phenomena in fungi. | microorganisms display a range of oscillatory phenomena that operate over different temporal scales. fourier analysis provides a compact description of such oscillations in terms of their frequency, magnitude and phase. however, in the majority of studies there is no explicit consideration of the spatial organisation of the oscillation. here we describe procedures and a software package to map oscillatory phenomena in microorganisms in both the time and frequency domains. key parameters of inter ... | 2007 | 17434770 |
| identification of substrates for transglutaminase in physarum polycephalum, an acellular slime mold, upon cellular mechanical damage. | transglutaminases are ca(2+)-dependent enzymes that post-translationally modify proteins by crosslinking or polyamination at specific polypeptide-bound glutamine residues. physarum polycephalum, an acellular slime mold, is the evolutionarily lowest organism expressing a transglutimase whose primary structure is similar to that of mammalian transglutimases. we observed transglutimase reaction products at injured sites in physarum macroplasmodia upon mechanical damage. with use of a biotin-labeled ... | 2007 | 17459100 |
| thuggacins, macrolide antibiotics active against mycobacterium tuberculosis: isolation from myxobacteria, structure elucidation, conformation analysis and biosynthesis. | two novel antibiotics, thuggacin a (1) and b (2), were isolated from the myxobacterium sorangium cellulosum. 1 and 2 are unique thiazole-containing macrolides with side chains on both sides of the lactone group. upon standing in solution, thuggacin a (1) rearranges by acyl migration of the lactone group to give a mixture with thuggacins b (2) and c (3). noes and vicinal coupling constants within the lactone ring provided distinct data for the generation of a structure model by pm3 calculations, ... | 2007 | 17506044 |
| emergence of morphological order in the network formation of physarum polycephalum. | emergence in a system appears through the interaction of its components, giving rise to higher order or complexity in the system. we tested for the presence of emergent properties in a biological system using the simplest biological entity of a unicellular organism; the plasmodium of physarum polycephalum, a giant unicellular amoeboid organism that forms a network-like tubular structure connecting its food sources. we let two plasmodium networks within a single cell interact with each other, and ... | 2007 | 17513034 |
| biosynthesis of (r)-beta-tyrosine and its incorporation into the highly cytotoxic chondramides produced by chondromyces crocatus. | the chondramides are mixed non-ribosomal peptide/polyketide secondary metabolites produced by the myxobacterium chondromyces crocatus cm c5, which exhibit strong cytotoxic activity. on the basis of their striking structural similarity to the marine depsipeptides jaspamides, the chondramides have been assumed to incorporate a (r)-beta-tyrosine moiety, an expectation we confirm here. thus, the recent sequencing of the chondramide biosynthetic gene cluster provided the opportunity to probe the shar ... | 2007 | 17545150 |
| physarum machines: encapsulating reaction-diffusion to compute spanning tree. | the physarum machine is a biological computing device, which employs plasmodium of physarum polycephalum as an unconventional computing substrate. a reaction-diffusion computer is a chemical computing device that computes by propagating diffusive or excitation wave fronts. reaction-diffusion computers, despite being computationally universal machines, are unable to construct certain classes of proximity graphs without the assistance of an external computing device. i demonstrate that the problem ... | 2007 | 17603779 |
| rna editing in physarum mitochondria: assays and biochemical approaches. | mitochondrial rnas in the myxomycete physarum polycephalum differ from the templates from which they are transcribed in defined ways. most transcripts contain nucleotides that are not present in their respective genes. these "extra" nucleotides are added during rna synthesis by an unknown mechanism. other differences observed between physarum mitochondrial rnas and the mitochondrial genome include nucleotide deletions, c to u changes, and the replacement of one nucleotide for another at the 5' e ... | 2007 | 17662840 |
| computational approaches to insertional rna editing. | insertional rna editing turns the task of locating edited genes and the editing sites within these genes into a challenge. computational techniques can greatly simplify this task by providing specific predictions for the location of genes and the editing sites within these genes that can be directly used to design primers for the experimental verification of these predictions. to obtain good predictions from computational methods, a substantial amount of sequence analysis is necessary that takes ... | 2007 | 17662841 |
| evolution of rna editing sites in the mitochondrial small subunit rrna of the myxomycota. | because of their unique and unprecedented character, it is often difficult to imagine how and why the different, diverse types of rna editing have evolved. information about the evolution of a particular rna editing system can be obtained by comparing rna editing characteristics in contemporary organisms whose phylogenetic relationships are known so that editing patterns in ancestral organisms can be inferred. this information can then be used to build models of the origins, constraints, variabi ... | 2007 | 17662842 |
| low rate of replication fork progression lengthens the replication timing of a locus containing an early firing origin. | invariance of temporal order of genome replication in eukaryotic cells and its correlation with gene activity has been well-documented. however, recent data suggest a relax control of replication timing. to evaluate replication schedule accuracy, we detailed the replicational organization of the developmentally regulated php locus that we previously found to be lately replicated, even though php gene is highly transcribed in naturally synchronous plasmodia of physarum. unexpectedly, bi-dimension ... | 2007 | 17717000 |
| alkaline phosphatase of physarum polycephalum is insoluble. | the plasmodia of physarum polycephalum grow as multinucleated cells in the presence of sufficient humidity and nutriment. under non-illuminating conditions, stresses such as low temperature or high concentrations of salts transform the plasmodia into spherules whereas dehydration induces sclerotization. some phosphatases including protein phosphatase and acid phosphatase have been purified from the plasmodia, but alkaline phosphatase remains to be elucidated. phosphatase of the plasmodia, spheru ... | 2008 | 17899011 |
| minimum-risk path finding by an adaptive amoebal network. | when two food sources are presented to the slime mold physarum in the dark, a thick tube for absorbing nutrients is formed that connects the food sources through the shortest route. when the light-avoiding organism is partially illuminated, however, the tube connecting the food sources follows a different route. defining risk as the experimentally measurable rate of light-avoiding movement, the minimum-risk path is exhibited by the organism, determined by integrating along the path. a model for ... | 2007 | 17930872 |
| a first glimpse at the transcriptome of physarum polycephalum. | physarum polycephalum, an acellular plasmodial species belongs to the amoebozoa, a major branch in eukaryote evolution. its complex life cycle and rich cell biology is reflected in more than 2500 publications on various aspects of its biochemistry, developmental biology, cytoskeleton, and cell motility. it now can be genetically manipulated, opening up the possibility of targeted functional analysis in this organism. | 2008 | 18179708 |
| amoebae anticipate periodic events. | when plasmodia of the true slime mold physarum were exposed to unfavorable conditions presented as three consecutive pulses at constant intervals, they reduced their locomotive speed in response to each episode. when the plasmodia were subsequently subjected to favorable conditions, they spontaneously reduced their locomotive speed at the time when the next unfavorable episode would have occurred. this implied the anticipation of impending environmental change. we explored the mechanisms underly ... | 2008 | 18232821 |
| sampling gene diversity across the supergroup amoebozoa: large est data sets from acanthamoeba castellanii, hartmannella vermiformis, physarum polycephalum, hyperamoeba dachnaya and hyperamoeba sp. | from comparative analysis of est data for five taxa within the eukaryotic supergroup amoebozoa, including two free-living amoebae (acanthamoeba castellanii, hartmannella vermiformis) and three slime molds (physarum polycephalum, hyperamoeba dachnaya and hyperamoeba sp.), we obtained new broad-range perspectives on the evolution and biosynthetic capacity of this assemblage. together with genome sequences for the amoebozoans dictyostelium discoideum and entamoeba histolytica, and including partial ... | 2008 | 18276190 |
| pedein a and b: production, isolation, structure elucidation and biological properties of new antifungal cyclopeptides from chondromyces pediculatus (myxobacteria). | two new secondary metabolites, named pedein a and b, were isolated from the cell mass of the myxobacterium chondromyces pediculatus. their planar structures were elucidated by spectroscopic methods, in particular 2d nmr as 24-membered cyclic hexapeptides composed of a variable tryptophan residue, glycine, sarcosine and three unusual hydroxy beta- and gamma-amino acids. the main component, pedein a, strongly inhibited the growth of yeasts and fungi, induced hemolysis of erythrocytes, and caused c ... | 2008 | 18305355 |
| synthesis, degradability, and drug releasing properties of methyl esters of fungal poly(beta,l-malic acid). | methyl esters of microbial poly(beta,l-malic acid) for conversion degrees of 25, 50, 75, and 100% were prepared by treatment of the polyacid with diazomethane. esterification proceeded with retention of the molecular weight of the parent polyacid and the copolymers displayed a blocky microstructure consisting of short segments of malic and methyl malate sequences. the thermal stability of the copolyesters was lower than those of the parent homopolymers and all of them were fairly crystalline wit ... | 2008 | 18322913 |
| biodegradable nanoparticles of partially methylated fungal poly(beta-l-malic acid) as a novel protein delivery carrier. | the preparation of nanoparticles from 75% methylated poly(beta-l-malic acid) is described. their degradation in aqueous environments was examined and the influence of ph and lipase on the rate of hydrolysis was evaluated. six proteins were used to estimate the loading efficiency of the nanoparticles. the amount of protein retained in the nanoparticles was found to depend on the acid/basic character of the protein. protein release from the loaded nanoparticles upon incubation in water under physi ... | 2008 | 18350538 |
| poly(malic acid) nanoconjugates containing various antibodies and oligonucleotides for multitargeting drug delivery. | nanoconjugates are emerging as promising drug-delivery vehicles because of their multimodular structure enabling them to actively target discrete cells, pass through biological barriers and simultaneously carry multiple drugs of various chemical nature. nanoconjugates have matured from simple devices to multifunctional, biodegradable, nontoxic and nonimmunogenic constructs, capable of delivering synergistically functioning drugs in vivo. this review mainly concerns the polycefin family of natura ... | 2008 | 18373429 |
| computational screen for spliceosomal rna genes aids in defining the phylogenetic distribution of major and minor spliceosomal components. | the rna molecules of the spliceosome are critical for specificity and catalysis during splicing of eukaryotic pre-mrna. in order to examine the evolution and phylogenetic distribution of these rnas, we analyzed 149 eukaryotic genomes representing a broad range of phylogenetic groups. rnas were predicted using high-sensitivity local alignment methods and profile hmms in combination with covariance models. the results provide the most comprehensive view so far of the phylogenetic distribution of s ... | 2008 | 18390578 |
| properties, intracellular localization, and stage-specific expression of membrane-bound beta-glucosidase, bglm1, from physarum polycephalum. | physarum polycephalum expresses a membrane-bound beta-glucosidase (bglm1) with a molecular mass of 130 kda. the primary structure of bglm1 consists of a glycosyl hydrolase family 3 domain at an amino-terminal domain and a carboxyl-terminal region without homology to the sequence of known glycosidases. the latter region contains two calx-beta motifs known as ca(2+)-binding sites; an rgd sequence, which is known to be a cell attachment sequence; and a transmembrane region. the molecular mass calcu ... | 2008 | 18395487 |
| production of the antifungal isochromanone ajudazols a and b in chondromyces crocatus cm c5: biosynthetic machinery and cytochrome p450 modifications. | 2008 | 18461592 | |
| automatic reconstruction of molecular and genetic networks from discrete time series data. | we apply a mathematical algorithm which processes discrete time series data to generate a complete list of petri net structures containing the minimal number of nodes required to reproduce the data set. the completeness of the list as guaranteed by a mathematical proof allows to define a minimal set of experiments required to discriminate between alternative network structures. this in principle allows to prove all possible minimal network structures by disproving all alternative candidate struc ... | 2008 | 18524471 |
| [expression silence of physarum polycephalum serine/arginine protein kinase by small interfering rna]. | serine/arginine protein kinases are specific kinase family for phosphorylating sr protein regulating alternative splicing of sr protein and its distribution, localization in the nucleus. however, it is unclear how physarum polycephalum serine/arginine protein kinase(psrpk) functions in the cells. in order to study its function, oligonucleotides for transcribing sirnas were designed and inserted into psiren-retroq vector to construct psiren-psrpk-1, psiren-psrpk-2, psiren-psrpk-3, psiren-psrpk-4, ... | 2008 | 18567503 |
| non-dna-templated addition of nucleotides to the 3' end of rnas by the mitochondrial rna polymerase of physarum polycephalum. | mitochondrial gene expression is necessary for proper mitochondrial biogenesis. genes on the mitochondrial dna are transcribed by a dedicated mitochondrial rna polymerase (mtrnap) that is encoded in the nucleus and imported into mitochondria. in the myxomycete physarum polycephalum, nucleotides that are not specified by the mitochondrial dna templates are inserted into some rnas, a process called rna editing. this is an essential step in the expression of these rnas, as the insertion of the nont ... | 2008 | 18573885 |
| electron microscopic and microprobe analysis of calciuminduced differentiation of the white mutant (lu887 x lu897) strain of physarum polycephalum. | differentiation of the white mutant (lu887 x lu897) strain of physarum polycephalum leading to spherule formation can be induced by cacl(2) if the concentration in the nutrient medium is increased by 5mm prior to the transfer to a non-nutrient salts medium. all stages previously reported for the typical (m(3)cvii) strain of physarum polycephalum from microplasmodia to spherules are seen but the mutant lacks the synchrony that the replacement technique induces in the typical strain. x-ray microan ... | 1989 | 18620272 |
| genome annotation in the presence of insertional rna editing. | insertional rna editing renders gene prediction very difficult compared to organisms without such rna editing. a case in point is the mitochondrial genome of physarum polycephalum in which only about one-third of the number of genes that are to be expected given its length are annotated. thus, gene prediction methods that explicitly take into account insertional editing are needed for successful annotation of such genomes. | 2008 | 18819938 |
| physarum polymalic acid hydrolase: recombinant expression and enzyme activation. | as a platform for syntheses of nanoconjugates in antitumor drug delivery, polymalic acid together with its tailoring specific exohydrolase is purified from plasmodium cultures of the slime mold physarum polycephalum, a member of the phylum myxomycota. polymalic acid hydrolase is expressed in an inactive form that functions as a molecular adapter for polymalic acid trafficking within the plasmodium and is activated only during secretion. activation follows specific protein tyrosine phosphorylatio ... | 2008 | 18845127 |
| environment-dependent morphology in plasmodium of true slime mold physarum polycephalum and a network growth model. | branching network growth patterns, depending on environmental conditions, in plasmodium of true slime mold physarum polycephalum were investigated. surprisingly, the patterns resemble those in bacterial colonies even though the biological mechanisms differ greatly. bacterial colonies are collectives of microorganisms in which individual organisms have motility and interact through nutritious and chemical fields. in contrast, the plasmodium is a giant amoeba-like multinucleated unicellular organi ... | 2009 | 18929578 |
| linker histone phosphorylation regulates global timing of replication origin firing. | despite the presence of linker histone in all eukaryotes, the primary function(s) of this histone have been difficult to clarify. knock-out experiments indicate that h1s play a role in regulation of only a small subset of genes but are an essential component in mouse development. here, we show that linker histone (h1) is involved in the global regulation of dna replication in physarum polycephalum. we find that genomic dna of h1 knock-down cells is more rapidly replicated, an effect due at least ... | 2009 | 19015270 |
| physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins. | physarum polycephalum expresses two closely related, calcium-independent noss (nitric oxide synthases). in our previous work, we showed that both noss are induced during starvation and apparently play a functional role in sporulation. in the present study, we characterized the genomic structures of both physarum noss, expressed both enzymes recombinantly in bacteria and characterized their biochemical properties. whereas the overall genomic organization of physarum nos genes is comparable with v ... | 2009 | 19046139 |
| [the role of phosphoinositide-3-kinase in controlling the shape and directional movement in physarum polycephalum plasmodium]. | the influence of wortmannin and ly294002, specific inhibitors of phosphoinosite-3-kinase, on the shape, motile behavior, and chemotaxis toward glucose has been investigated in physarum polycephalum plasmodium, a multinuclear amoeboid cell with the autooscillatory mode of motion. both inhibitors were shown to cause a reduction of the plasmodium frontal edge and a decrease in the efficiency of mass transfer during migration. they also suppress chemotaxis toward glucose and eliminate characteristic ... | 2008 | 19137682 |
| [cytomechanics of oscillatory contractions. measurement of active mechanical properties of physarum polycephalum plasmodium strands]. | the aim of this series of studies is to elucidate the role of mechanical stresses in the processes of cell activation. the experiments were done with a huge unicellular organism, the physarum polycephalum plasmodium, which is a classical object in studies of the nonmuscle motility. the contractile properties of this amoeboid cell were investigated with the help of an inexpensive electronic-mechanical measuring system. a short description of this device is presented, which allows one to maintain ... | 2008 | 19137691 |
| unusual chemistry in the biosynthesis of the antibiotic chondrochlorens. | the antibiotic chondrochlorens a and b from the myxobacterium chondromyces crocatus cm c5 incorporate several unusual structural features, notable among them a shared chloro-hydroxy-styryl functionality and the ethoxy group of chondrochloren b. our analysis of the chondrochloren gene cluster by targeted gene inactivation coupled with assays in vitro has shed significant light on the biosynthesis of these metabolites. chlorination of tyrosine occurs early in the pathway, likely on a peptidyl carr ... | 2009 | 19171307 |
| discovery of additional members of the tyrosine aminomutase enzyme family and the mutational analysis of cmdf. | the tyrosine aminomutase (tam) cmdf converts l-tyr preferentially to (r)-beta-tyr--a biosynthetic building block subsequently incorporated into the highly cytotoxic chondramides by the myxobacterium chondromyces crocatus. together with the similar enzymes sgcc4 from streptomyces globisporus and mdpc4 from actinomadura madurae, which preferentially produce (s)-beta-tyr, cmdf belongs to a novel 2,3-aminomutase enzyme family closely related to the aromatic amino acid ammonia lyase. although conside ... | 2009 | 19222035 |
| cdna cloning and life-cycle stage-specific expression of coronin from physarum polycephalum. | coronin cdna was cloned from the plasmodia of physarum polycephalum. the amino acid sequence deduced from the cdna was comprised of 449 residues and showed 60% identity to that of dictyostelium discoideum coronin. southern blot analysis suggested that the coronin gene present in the p. polycephalum genome might be a single copy. coronin was expressed in diploid plasmodia, while it was not detected in haploid amoebae or spores. | 2009 | 19270411 |
| the p1 and p1' residue specificities of physarolisin i, a serine-carboxyl peptidase from the true slime mold physarum polycephalum. | the p1 and p1' residue specificities of physarolisin i were investigated using combinatorial peptide substrates. the results indicated that certain hydrophobic residues and acidic residues are preferred at the p1 position and some hydrophobic residues at the p1' position. this p1 specificity, different from other serine-carboxyl peptidases, appears to be explained partially by the nature of the s1 subsite residues. | 2009 | 19420694 |
| distinct roles for sequences upstream of and downstream from physarum editing sites. | rnas in the mitochondria of physarum polycephalum contain nonencoded nucleotides that are added during rna synthesis. essentially all steady-state rnas are accurately and fully edited, yet the signals guiding these precise nucleotide insertions are presently unknown. to localize the regions of the template that are required for editing, we constructed a series of chimeric templates that substitute varying amounts of dna either upstream of or downstream from c insertion sites. remarkably, all seq ... | 2009 | 19605532 |
| a novel physarum polycephalum sr protein kinase specifically phosphorylates the rs domain of the human sr protein, asf/sf2. | a 1591-bp cdna of a serine-rich protein kinase (srpk)-like protein has been identified in physarum polycephalum (genbank accession no. dq140379). the cdna contains two repeat sequences at bp 1-153 and bp 395-547. the encoding sequence is 56% homologous to human srpk1 and is named physarum srpk (psrpk). consistent with other srpks, the consensus motifs of psrpk are within the two conserved domains (cds). however, divergent motifs between the n-terminal and cds are much shorter than the correspond ... | 2009 | 19657567 |
| identification of a nuclear localization motif in the serine/arginine protein kinase psrpk of physarum polycephalum. | serine/arginine (sr) protein-specific kinases (srpks) are conserved in a wide range of organisms, from humans to yeast. studies showed that srpks can regulate the nuclear import of sr proteins in cytoplasm, and regulate the sub-localization of sr proteins in the nucleus. but no nuclear localization signal (nls) of srpks was found. we isolated an srpk-like protein psrpk (genbank accession no. dq140379) from physarum polycephalum previously, and identified a nls of psrpk in this study. | 2009 | 19703313 |
| doppler oct imaging of cytoplasm shuttle flow in physarum polycephalum. | the doppler optical coherence tomography technique was applied to image the oscillatory dynamics of protoplasm in the strands of the plasmodium of slime mould physarum polycephalum. radial contractions of the gel-like walls of the strands and the velocity distributions in the sol-like endoplasm streaming along the plasmodial strands are imaged. the motility inhibitor effect of carbon dioxide on the cytoplasm shuttle flow and strand-wall contraction is shown. the optical attenuation coefficient o ... | 2009 | 19743444 |
| [transient expression in microplasmodia of physarum polycephalum]. | the plasmodium of physarum polycephalum is a suitable eukaryotic cell for cell cycle investigation, but there is no compatible transient expression system for the plasmodium. using the promoter and terminator of ardc actin of physarum polycephalum substituted the cmv ie and sv40 polya of plasmid pdsredl-n1, using cassette pardc-mcs-dsred1-tardc substituted the cassette pardc-hph-tardc of plasmid ptb38, we constructed plasmids pxm1 and pxm2 for transient expression of red fluorescent protein (rfp ... | 2009 | 19777812 |