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cycles of light and dark co-ordinate reversible colony differentiation in listeria monocytogenes.recently, several light receptors have been identified in non-phototrophic bacteria, but their physiological roles still remain rather elusive. here we show that colonies of the saprophytic bacterium listeria monocytogenes undergo synchronized multicellular behaviour on agar plates, in response to oscillating light/dark conditions, giving rise to alternating ring formation (opaque and translucent rings). on agar plates, bacteria from opaque rings survive increased levels of reactive oxygen speci ...201323331346
disruption of lmo1386, a putative dna translocase gene, affects biofilm formation of listeria monocytogenes on abiotic surfaces.the distribution and survival of listeria monocytogenes (l. monocytogenes) in food processing environment is linked to its ability to form biofilms, however the genetic mechanisms remain unclear. in our previous study, a himar1 mariner-based transposon mutagenesis was performed and 42 mutants were confirmed to have reduced biofilm formation. among the 42 biofilm deficient mutants, two isolates (s25-10c and s55-1d) harbored single insertion in lmo1386, a gene encoding a putative dna translocase. ...201323333341
antibacterial activity of veronica montana l. extract and of protocatechuic acid incorporated in a food system.this study was designed to evaluate the antibacterial activity of the veronica montana l. water extract and its main phenolic compound, protocatechuic acid. the antibacterial activity was determined by microdilution assay against six strains of gram-positive and gram-negative bacteria. listeria monocytogenes was the most sensitive of the tested bacterial species. antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using l. monoc ...201323333716
survival of food-borne pathogens on natural black table olives after post-processing contamination.the aim of this study was to evaluate the survival of salmonella, escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus on natural black olives during aerobic storage without brine and to conclude about the safety of this fermented food. fermented black olives (ph 3.95; nacl 6.02%) were artificially inoculated with 4 strains of salmonella enterica ser. enteritidis, 4 strains of s. enterica ser. typhimurium, 3 strains of e. coli o157:h7, 3 strains of l. monocytogenes, and 3 ...201323334099
reassessment of the listeria monocytogenes pan-genome reveals dynamic integration hotspots and mobile genetic elements as major components of the accessory genome.listeria monocytogenes is an important food-borne pathogen and model organism for host-pathogen interaction, thus representing an invaluable target considering research on the forces governing the evolution of such microbes. the diversity of this species has not been exhaustively explored yet, as previous efforts have focused on analyses of serotypes primarily implicated in human listeriosis. we conducted complete genome sequencing of 11 strains employing 454 gs flx technology, thereby achieving ...201323339658
retraction statement: performance of three culture media commonly used for detecting listeria monocytogenes. 201223339828
mudpit profiling reveals a link between anaerobic metabolism and the alkaline adaptive response of listeria monocytogenes egd-e.listeria monocytogenes is a foodborne human pathogen capable of causing life-threatening disease in susceptible populations. previous proteomic analysis we performed demonstrated that different strains of l. monocytogenes initiate a stringent response when subjected to alkaline growth conditions. here, using multidimensional protein identification technology (mudpit), we show that in l. monocytogenes egd-e this response involves an energy shift to anaerobic pathways in response to the extracellu ...201323342094
tn6198, a novel transposon containing the trimethoprim resistance gene dfrg embedded into a tn916 element in listeria monocytogenes.to characterize tn6198, a novel conjugative transposon from the clinical listeria monocytogenes strain tth-2007, which contains the tetracycline and trimethoprim resistance genes tet(m) and dfrg, respectively, and to assess its transferability in vitro and in situ.201323344576
pathogenesis of listeriosis during pregnancy.listeria monocytogenes causes several clinical manifestations in humans and domestic animals. this bacterium is a saprophyte in soil and ensiled feeds, which are sources of infection for food producing animals (i.e. ruminants). the most common route of infection for people is via ingestion of contaminated ready-to-eat food products such as produce, soft cheeses and deli meats. in the united states, l. monocytogenes causes relatively few cases of clinical disease compared to other food-borne path ...201323347534
fluorescence amplified fragment length polymorphism compared to pulsed field gel electrophoresis for listeria monocytogenes subtyping.listeriosis is a severe infection which mainly affects pregnant women, neonates and immuno-compromised adults. anses's laboratory for food safety has been the european union reference laboratory (eurl) for l. monocytogenes in the food chain since 2006. pulsed field gel electrophoresis (pfge) is routinely used in the eurl for the surveillance of l. monocytogenes isolated from foods, animals and the environment. one of the main eurl activities is to evaluate alternative molecular subtyping methods ...201323347599
cell-mediated killing of listeria monocytogenes by leucocin c producing escherichia coli.listeria monocytogenes is a foodborne pathogen causing listeriosis. listeria in foods can be inhibited with bacteriocins or bacteriocin producing cultures. the aim of this study was to enhance the killing of l. monocytogenes by binding bacteriocin producing escherichia coli cells to listeria cells. antilisterial e. coli was obtained by transferring leucocin c production from leuconostoc carnosum 4010. for binding of e. coli cells to listeria cells, the listeria phage endolysin plyp35 cell wall b ...201323348073
brain infection and activation of neuronal repair mechanisms by the human pathogen listeria monocytogenes in the lepidopteran model host galleria mellonella.listeria monocytogenes the causative agent of the foodborne disease listeriosis in humans often involves fatal brainstem infections leading to meningitis and meningoencephalitis. we recently established the larvae of the greater wax moth (galleria mellonella) as a model host for the investigation of l. monocytogenes pathogenesis and as a source of peptides exhibiting anti-listeria-activity. here we show that g. mellonella can be used to study brain infection and its impact on larval development ...201323348912
in vitro antimicrobial activity of pistachio (pistacia vera l.) polyphenols.we investigated the antimicrobial properties of polyphenol-rich fractions derived from raw shelled and roasted salted pistachios. american type culture collection (atcc), food and clinical isolates, of gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, pseudomonas mirabilis), gram-positive bacteria (listeria monocytogenes, enterococcus hirae, enterococcus faecium, bacillus subtilis, staphylococcus epidermidis, staphylococcus aureus), the yeasts candida albicans and candida parapsi ...201323350629
lateral-flow enzyme immunoconcentration for rapid detection of listeria monocytogenes.lateral-flow enzyme immunochromatography coupled with an immunomagnetic step was developed for rapid detection of listeria monocytogenes in food matrices. the target bacteria was first separated and concentrated by magnetic nanoparticles containing the enzyme and directly applied to the assay system to induce an antigen-antibody reaction without any additional steps. the color signals produced by an enzyme-substrate reaction at a specific site on the immunostrip were found to be directly proport ...201323354582
antimicrobial and antifungal activities of lactobacillus curvatus strain isolated from homemade azerbaijani cheese.the aims of this study were to characterize inhibitory activity spectra, some probiotic properties and safety of lactobacillus curvatus a61 for its future application in production of fermented foods. the studied strain was isolated from traditional homemade cheese manufactured in azerbaijan. the cell-free supernatant of culture of lb. curvatus a61 inhibited the growth of tested lab, as well as of listeria monocytogenes and bacillus cereus strains. the strain presented antifungal activity and in ...201323357316
cd11a regulates effector cd8 t cell differentiation and central memory development in response to infection with listeria monocytogenes.β2 (cd18) integrins with α-chains cd11a, -b, -c, and -d are important adhesion molecules necessary for leukocyte migration and cellular interactions. cd18 deficiency leads to recurrent bacterial infections and poor wound healing due to reduced migration of leukocytes to inflammatory sites. cd8 t cells also upregulate cd11a, cd11b, and cd11c upon activation. however, the role these molecules play for cd8 t cells in vivo is not known. to determine the function of individual β2 integrins, we examin ...201323357382
involvement of toso in activation of monocytes, macrophages, and granulocytes.rapid activation of immune responses is necessary for antibacterial defense, but excessive immune activation can result in life-threatening septic shock. understanding how these processes are balanced may provide novel therapeutic potential in treating inflammatory disease. fc receptors are crucial for innate immune activation. however, the role of the putative fc receptor for igm, known as toso/faim3, has to this point been unclear. in this study, we generated toso-deficient mice and used them ...201323359703
isolation, identification and characterisation of three novel probiotic strains (lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036) from the faeces of exclusively breast-fed infants.the aim of the present study was to isolate, identify and characterise novel strains of lactic acid bacteria and bifidobacteria with probiotic properties from the faeces of exclusively breast-fed infants. of the 4680 isolated colonies, 758 exhibited resistance to low ph and tolerance to high concentrations of bile salts; of these, only forty-two exhibited a strong ability to adhere to enterocytes in vitro. the identities of the isolates were confirmed by 16s ribosomal rna (rrna) sequencing, whic ...201323360881
rapid detection of several foodborne pathogens by f0f1-atpase molecular motor biosensor.f0f1-atpase within chromatophore was constructed as a molecular motor biosensor through ε-subunit antibody-biotin-streptavidin-biotin-ac5-sulfo-osu system. based on probe-dna specific binding, dna of several foodborne pathogens listeria monocytogenes, salmonella typhimurium, vibrio parahaemolyticus and vibrio cholerae was specifically captured by f0f1-atpase molecular motor biosensors. loads of dna decreased the rotation rate of f0f1-atpase, and led to the decrease of atp synthesis. the detectio ...201323361046
listeria monocytogenes in donated platelets: a potential transfusion-transmitted pathogen intercepted through screening.bacterial contamination of blood components is a potentially life-threatening complication of transfusions. in october 2005, the centers for disease control and prevention (cdc) noted four listeria monocytogenes (lm) isolates cultured from four different units of donated apheresis platelets (plts) among lm isolates sent to the cdc national listeria reference laboratory for subtyping as part of routine surveillance activities.201323362824
probiotic-mediated competition, exclusion and displacement in biofilm formation by food-borne pathogens.the objective of this study was to examine the inhibitory effect of probiotic strains on pathogenic biofilm formation in terms of competition, exclusion and displacement. probiotic strains (lactobacillus acidophilus kacc 12419, lact. casei kacc 12413, lact. paracasei kacc 12427 and lact. rhamnosus kacc 11953) and pathogens (salmonella typhimurium kccm 40253 and listeria monocytogenes kacc 12671) were used to evaluate the auto-aggregation, hydrophobicity and biofilm formation inhibition. the high ...201323362863
bilateral optic perineuritis as the presenting feature of giant cell arteritis.optic perineuritis (opn) is an uncommon inflammatory disorder of the optic nerve sheath. most cases are idiopathic, though opn can rarely occur as a manifestation of vasculitic diseases. we describe the case of a 74-year-old caucasian man presenting with diplopia and bilateral visual loss. a brain mri showed bilateral opn without involvement of other structures. all the tests performed to investigate opn's aetiology showed negative results. considering clinical presentation and neuroimaging find ...201323365154
trajectories of listeria-type motility in two dimensions.force generated by actin polymerization is essential in cell motility and the locomotion of organelles or bacteria such as listeria monocytogenes. both in vivo and in vitro experiments on actin-based motility have observed geometrical trajectories including straight lines, circles, s-shaped curves, and translating figure eights. this paper reports a phenomenological model of an actin-propelled disk in two dimensions that generates geometrical trajectories. our model shows that when the evolution ...201223367971
novel antibody/gold nanoparticle/magnetic nanoparticle nanocomposites for immunomagnetic separation and rapid colorimetric detection of staphylococcus aureus in milk.we demonstrated the new antibody/gold nanoparticle/magnetic nanoparticle nanocomposites (antibody/aunp/mnps) and their application in the detection of the foodborne pathogen, staphylococcus aureus (s. aureus), in milk. the nanocomposites were synthesized by coating the mnps with bovine serum albumin (bsa) then adsorbing the aunps and anti-s. aureus antibodies on their surface. using the completed immunomagnetic nanostructures, s. aureus inoculated in the milk sample was captured and isolated fro ...201323370174
pathogenic bacteria in finnish bulk tank milk.while the quality of raw cow milk in finland is known for its high hygienic standard, with the national average total bacterial count being below 10(4) cfu/ml annually, the prevalence of pathogenic bacteria in finnish raw milk is underreported. the aim of this study was to determine the occurrence of listeria monocytogenes, thermophilic campylobacter spp., salmonella spp., stx-positive escherichia coli (stec), coagulase-positive staphylococci, yersinia spp., and bacillus cereus group in raw cow ...201323373473
in vivo activity of nisin a and nisin v against listeria monocytogenes in mice.lantibiotics are post-translationally modified antimicrobial peptides, of which nisin a is the most extensively studied example. bioengineering of nisin a has resulted in the generation of derivatives with increased in vitro potency against gram-positive bacteria. of these, nisin v (containing a met21val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including listeria monocytogenes. however, this increased ...201323374279
the surface proteins inla and inlb are interdependently required for polar basolateral invasion by listeria monocytogenes in a human model of the blood-cerebrospinal fluid barrier.the gram-positive bacterium listeria monocytogenes can enter the human central nervous system and cause life-threatening meningitis. during this process the pathogen has to invade and cross diverse cellular barriers involving the functions of the surface proteins internalin (inla) and inlb. whereas the internalin-dependent crossing of the intestinal epithelium and the fetoplacental barrier have been subject to intensive investigation, limited research elucidating the crossing of the human blood- ...201323376167
genetic determinants for cadmium and arsenic resistance among listeria monocytogenes serotype 4b isolates from sporadic human listeriosis patients.in listeria monocytogenes serotype 4b isolates from sporadic listeriosis, heavy metal resistance was primarily encountered in certain clonal groups (eci, ecii, and ecia). all arsenic-resistant isolates harbored the arsenic resistance cassette previously identified in pli100; ecia harbored additional arsenic resistance genes and a novel cadmium resistance determinant in a conserved chromosomal locus.201323377929
a defined, glucose-limited mineral medium for the cultivation of listeria spp.members of the genus listeria are fastidious bacteria with respect to their nutritional requirements, and several minimal media described in the literature fail to support growth of all listeria spp. furthermore, strict limitation by a single nutrient, e.g., the carbon source, has not been demonstrated for any of the published minimal media. this is an important prerequisite for defined studies of growth and physiology, including "omics." based on a theoretical analysis of previously published m ...201323377938
sting/mpys mediates host defense against listeria monocytogenes infection by regulating ly6c(hi) monocyte migration.mpys (also known as sting, mita, and tmem173) is a type i ifn stimulator that is essential for host defense against dna virus infection and appears important in defense against certain bacteria. the in vivo significance and mechanisms by which mpys mediates host defense against nonviral pathogens are unknown. using an mpys-deficient mouse (tmem173(<tm1camb>)), we determined that, distinct from the ifnar(-/-) mice, mpys deficiency leads to increased bacterial burden in the liver upon listeria mon ...201323378430
acta promotes listeria monocytogenes aggregation, intestinal colonization and carriage.listeria monocytogenes (lm) is a ubiquitous bacterium able to survive and thrive within the environment and readily colonizes a wide range of substrates, often as a biofilm. it is also a facultative intracellular pathogen, which actively invades diverse hosts and induces listeriosis. so far, these two complementary facets of lm biology have been studied independently. here we demonstrate that the major lm virulence determinant acta, a prfa-regulated gene product enabling actin polymerization and ...201323382675
bacteriocin production by bifidobacterium spp. a review.bacteriocins are ribosomally-synthesized antibacterial peptides. these compounds are produced by a broad variety of different bacteria belonging mainly to the genus bifidobacterium, to which health promoting properties have frequently been attributed. however, despite the fact that the identification of bifidobacterium-associated bacteriocins was first reported in 1980 and that they exhibit antimicrobial activity against pathogenic microorganisms such as listeria monocytogenes, clostridium perfr ...201323384878
sculpting mhc class ii-restricted self and non-self peptidome by the class i ag-processing machinery and its impact on th-cell responses.it is generally assumed that the mhc class i antigen (ag)-processing (cap) machinery - which supplies peptides for presentation by class i molecules - plays no role in class ii-restricted presentation of cytoplasmic ags. in striking contrast to this assumption, we previously reported that proteasome inhibition, tap deficiency or eraap deficiency led to dramatically altered t helper (th)-cell responses to allograft (hy) and microbial (listeria monocytogenes) ags. herein, we tested whether altered ...201323386199
an attenuated listeria monocytogenes vector primes more potent simian immunodeficiency virus-specific mucosal immunity than dna vaccines in mice.a human immunodeficiency virus type 1 (hiv-1) vaccine that induces potent immune responses in the gastrointestinal mucosa would be highly desirable. here we show that attenuated recombinant listeria monocytogenes, administered orally utilizing its natural route of infection, induces potent mucosal as well as systemic immune responses in mice. moreover, these responses can be boosted efficiently with replication-incompetent adenoviral vectors. l. monocytogenes elicited more potent simian immunode ...201323388715
improving efficiency of viability-pcr for selective detection of live cells.viability pcr (v-pcr) as a method to selectively detect intact live cells has gained considerable interest over the last years with an increasing number of applications. the principle is based on treatment of microbiological samples with a viability dye prior to extraction of genomic dna and its amplification. the dye is selectively taken up by membrane-compromised dead cells resulting in the degradation of their dna upon light exposure and therefore inhibition of amplification. although the tre ...201323389080
monoclonal antibodies recognizing the surface autolysin ispc of listeria monocytogenes serotype 4b: epitope localization, kinetic characterization, and cross-reaction studies.listeria monocytogenes serotype 4b is responsible for a high percentage of fatal cases of food-borne infection. in a previous study, we created 15 monoclonal antibodies (mabs) against a ≈ 77 kda antigen that is associated with the cell surface of live l. monocytogenes serotype 4b cells. here we report an extensive characterization of these mabs to further their development as diagnostic reagents. the ≈ 77 kda target antigen was identified by mass spectrometry and n-terminal sequencing to be ispc ...201323390515
effects of high hydrostatic pressure and varying concentrations of sodium nitrite from traditional and vegetable-based sources on the growth of listeria monocytogenes on ready-to-eat (rte) sliced ham.the objective of this study was to determine the effect the source of added nitrite and high hydrostatic pressure (hhp) had on the growth of listeria monocytogenes on ready-to-eat (rte) sliced ham. use of 600mpa hhp for 3min resulted in an immediate 3.9-4.3log cfu/g reduction in l. monocytogenes numbers, while use of 400mpa hhp (3min) provided less than 1log cfu/g reduction. with the 600mpa hhp treatment, sliced ham with a conventional concentration of sodium nitrite (200ppm) was not different i ...201323391864
physicochemical quality and chemical safety of chlorine as a reconditioning agent and wash water disinfectant for fresh-cut lettuce washing.chlorine was assessed as a reconditioning agent and wash water disinfectant in the fresh-cut produce industry. artificial fresh-cut lettuce wash water, made from butterhead lettuce, was used for the experiments. in the reconditioning experiments, chlorine was added to artificial wash water inoculated with escherichia coli o157 (6 log cfu/ml). regression models were constructed based on the inactivation data and validated in actual wash water from leafy vegetable processing companies. the model t ...201323396332
processing of recombinant listeria monocytogenes proteins for mhc class i presentation follows a dedicated, high-efficiency pathway.cd8(+) t lymphocytes recognize short peptides of ∼8-10 aa bound to mhc class i molecules (pmhc) on the surface of apcs. these peptides can be generated from either endogenous proteins synthesized by the biosynthetic machinery of the presenting cell or from exogenously sourced proteins. because much of the research characterizing the mhc class i processing pathway has focused on endogenously synthesized proteins, it is not known whether differences exist in the processing pathway followed by endo ...201323396941
listeriolysin o as a strong immunogenic molecule for the development of new anti-tumor vaccines.the pore-forming toxin listeriolysin o (llo), which is produced by listeria monocytogenes, mediates bacterial phagosomal escape and facilitates bacterial multiplication during infection. this toxin has recently gained attention because of its confirmed role in the controlled and specific modulation of the immune response. currently, cancer immunotherapies are focused on conquering the immune tolerance induced by poorly immunogenic tumor antigens and eliciting strong, lasting immunological memory ...201323399758
impact of the listeria monocytogenes protein inlc on infection in mice.the bacterial pathogen listeria monocytogenes causes serious food-borne illnesses in pregnant women and the immunocompromised. l. monocytogenes promotes its internalization into host epithelial cells and then uses an f-actin-dependent motility process to spread from infected cells to surrounding healthy cells. in cultured enterocytes, efficient spread of l. monocytogenes requires the secreted bacterial protein inlc. inlc promotes dissemination by physically interacting with and antagonizing the ...201323403554
complete genome sequence of listeria monocytogenes ll195, a serotype 4b strain from the 1983-1987 listeriosis epidemic in switzerland.the complete genome sequence of listeria monocytogenes ll195, a serotype 4b clinical strain isolated during the 1983-1987 listeriosis epidemic in switzerland, is presented.201323405339
a potential new pathway for pd-l1 costimulation of the cd8-t cell response to listeria monocytogenes infection.programmed death ligand-1 (pd-l1) is an important negative regulator of t cell immune responses via interactions with pd-1 and cd80. however, pd-l1 can also act as a positive costimulator, but the relevant counterreceptor is not known. we analyzed the role of pd-l1 in cd8-t cell responses to infection with listeria monocytogenes (lm) or vesicular stomatitis virus (vsv). pd-l1 blockade impaired antigen-specific cd8 effector t cell expansion in response to lm, but not to vsv infection, particularl ...201323409193
comprehensive update of dalbavancin activity when tested against uncommonly isolated streptococci, corynebacterium spp., listeria monocytogenes, and micrococcus spp. (1357 strains).dalbavancin is an investigational lipoglycopeptide having an extended serum elimination half-life allowing once-weekly dosing. data from testing 1357 strains of uncommonly isolated species expand the dalbavancin spectrum details as follows (mic50/90): β-haemolytic streptococcal serogroups c, f, and g (≤0.03/≤0.03 μg/ml), 7 viridans group of streptococci (≤0.03/≤0.03-0.06 μg/ml), 5 corynebacterium spp. (0.06/0.12 μg/ml), listeria monocytogenes (0.06/0.12 μg/ml), and micrococcus spp. (≤0.03/≤0.03 ...201323414746
comparative evaluation on fatty acid and matricaria recutita essential oil incorporated into casein-based film.sodium caseinate composite films containing lipids-oleic acid (oa), stearic acid (sa), or matricaria recutita essential oil (meo) - were prepared through emulsification and their physical, thermal, mechanical, and barrier properties were evaluated and compared. furthermore, their antimicrobial effectiveness against listeria monocytogenes, staphylococcus aureus, and escherichia coli was studied. emulsified films were softer, less rigid, and more stretchable than pure films. the films' water vapor ...201323415659
mutants of listeriolysin o for enhanced liposomal delivery of macromolecules.delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of the cargo. listeriolysin o is a key virulence factor of listeria monocytogenes that forms pores in the endosomal membrane, ultimately allowing the bacterium to escape into the cytoso ...201323416330
biotechnological production of phenyllactic acid and biosurfactants from trimming vine shoot hydrolyzates by microbial coculture fermentation.coculture fermentations show advantages for producing food additives from agroindustrial wastes, considering that different specified microbial strains are combined to improve the consumption of mixed sugars obtained by hydrolysis. this technology dovetails with both the growing interest of consumers towards the use of natural food additives and with stricter legislations and concern in developed countries towards the management of wastes. the use of this technology allows valorization of both c ...201323417349
hlya gene-based sensitive detection of listeria monocytogenes using a novel cantilever sensor.piezoelectric cantilever sensors are shown to exhibit sensitive and selective detection based on an identifying gene from genomic extract at ~10(2)-10(3) cells of foodborne pathogen, listeria monocytogenes (lm). the study consists of two parts: tests with synthetic genes and experiments starting with whole lm cells. a probe designed for the virulence hemolysin gene, hlya, was immobilized on the gold-coated sensor, and hybridization detection of a synthetic target (based on hlya) is shown to span ...201323418904
i kappa b kinase alpha (ikkα) activity is required for functional maturation of dendritic cells and acquired immunity to infection.dendritic cells (dc) are required for priming antigen-specific t cells and acquired immunity to many important human pathogens, including mycobacteriuim tuberculosis (tb) and influenza. however, inappropriate priming of auto-reactive t cells is linked with autoimmune disease. understanding the molecular mechanisms that regulate the priming and activation of naïve t cells is critical for development of new improved vaccines and understanding the pathogenesis of autoimmune diseases. the serine/thr ...201323422957
chorioamnionitis caused by listeria monocytogenes: a case report of ultrasound features of fetal infection.maternal listeriosis is often associated with mild symptoms for the patient, but fetal infection can lead to severe adverse perinatal outcome. the most described antenatal symptoms are reduced fetal movements and an abnormal fetal heart rate trace. we present a case of fetal listeriosis suspected by ultrasound findings of fetal gastrointestinal compromise, neonatal diagnosis and outcome.201323429225
kinase suppressor of ras 1 is not required for the generation of regulatory and memory t cells.the mammalian target of rapamycin (mtor) kinase is a critical regulator of the differentiation of helper and regulatory cd4+ t cells, as well as memory cd8+ t cells. in this study, we investigated the role of the erk signaling pathway in regulating mtor activation in t cells. we showed that activation of erk following tcr engagement is required for sustained mtor complex 1 (mtorc1) activation. absence of kinase suppressor of ras 1 (ksr1), a scaffold protein of the erk signaling pathway, or inhib ...201323431403
prevalence and characteristics of listeria monocytogenes in bovine colostrum in japan.this study was conducted to determine the prevalence and characteristics of listeria monocytogenes in bovine colostrum in japan. we collected bovine colostrum samples from 210 dams from 21 dairy farms in hokkaido prefecture (japan) between march and june 2009. l. monocytogenes was detected in samples from 6 (28.6%) of the 21 farms. of the 210 samples, 16 (7.6%) were positive for l. monocytogenes. we recovered 80 l. monocytogenes isolates; 44 (55%) isolates were classified as serotype 1/2b and 36 ...201323433372
tracking cross-contamination transfer dynamics at a mock retail deli market using glogerm.ready-to-eat (rte) deli meats are considered a food at high risk for causing foodborne illness. deli meats are listed as the highest risk rte food vehicle for listeria monocytogenes. cross-contamination in the retail deli market may contribute to spread of pathogens to deli meats. understanding potential cross-contamination pathways is essential for reducing the risk of contaminating various products. the objective of this study was to track cross-contamination pathways through a mock retail del ...201323433375
food microbe tracker: a web-based tool for storage and comparison of food-associated microbes.large amounts of molecular subtyping information are generated by the private sector, academia, and government agencies. however, use of subtype data is limited by a lack of effective data storage and sharing mechanisms that allow comparison of subtype data from multiple sources. currently available subtype databases are generally limited in scope to a few data types (e.g., mlst. net) or are not publicly available (e.g., pulsenet). we describe the development and initial implementation of food m ...201323433376
genome sequencing identifies two nearly unchanged strains of persistent listeria monocytogenes isolated at two different fish processing plants sampled 6 years apart.listeria monocytogenes is a food-borne human-pathogenic bacterium that can cause infections with a high mortality rate. it has a remarkable ability to persist in food processing facilities. here we report the genome sequences for two l. monocytogenes strains (n53-1 and la111) that were isolated 6 years apart from two different danish fish processers. both strains are of serotype 1/2a and belong to a highly persistent dna subtype (random amplified polymorphic dna [rapd] type 9). we demonstrate us ...201323435887
a heterologous prime-boost vaccination strategy comprising the francisella tularensis live vaccine strain capb mutant and recombinant attenuated listeria monocytogenes expressing f. tularensis iglc induces potent protective immunity in mice against virulent f. tularensis aerosol challenge.francisella tularensis, the causative agent of tularemia, is a category a bioterrorism agent. a vaccine that is safer and more effective than the currently available unlicensed f. tularensis live vaccine strain (lvs) is needed to protect against intentional release of aerosolized f. tularensis, the most dangerous type of exposure. in this study, we employed a heterologous prime-boost vaccination strategy comprising intradermally administered lvs δcapb (highly attenuated capb-deficient lvs mutant ...201323439306
proteomic analysis shows that individual listeria monocytogenes strains use different strategies in response to gastric stress.ingestion of contaminated dairy products, in particular soft cheese, is one of the major routes of infection by the human pathogen listeria monocytogenes. during cheese processing, this foodborne pathogen is exposed to sublethal acid and osmotic stress conditions, which may induce tolerance responses and influence subsequent survival in the gastric tract. the aim of the current study was to evaluate the impact on a l. monocytogenes cheese isolate (serotype 4b) and two cheese dairy isolates (t8, ...201323441912
effect of soy and milk protein-related compounds on listeria monocytogenes infection in human enterocyte caco-2 cells and a/j mice.listeria monocytogenes causes listeriosis in humans, mainly through the consumption of ready-to-eat foods such as cheese. immunocompromised persons, the elderly, and pregnant women and their fetuses or newborns are at the highest risk for the infection. we examined the effects of dietary milk-casein (mc) and soy-protein (sp), and their digested compounds tryptone (tp) and phytone peptone (pp), respectively, on l. monocytogenes invasion and infection in human enterocyte-like caco-2 cells and a/j ...201223442612
antimicrobial activity of essential oils from mediterranean aromatic plants against several foodborne and spoilage bacteria.the antimicrobial activity of essential oils extracted from a variety of aromatic plants, often used in the portuguese gastronomy was studied in vitro by the agar diffusion method. the essential oils of thyme, oregano, rosemary, verbena, basil, peppermint, pennyroyal and mint were tested against gram-positive (listeria monocytogenes, clostridium perfringens, bacillus cereus, staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and staphylococcus epidermidis) and gram-negative stra ...201323444311
peptide-bacteria interactions using engineered surface-immobilized peptides from class iia bacteriocins.specificity of the class iia bacteriocin leucocin a (leua), an antimicrobial peptide active against gram-positive bacteria, including listeria monocytogenes , is known to be dictated by the c-terminal amphipathic helical region, including the extended hairpin-like structure. however, its specificity when attached to a substrate has not been investigated. exploiting properties of leua, we have synthesized two leua derivatives, which span the amphipathic helical region of the wild-type leua, consi ...201323445325
survival of listeria monocytogenes and salmonella enteritidis in sea bream (sparus aurata) fillets packaged under enriched co2 modified atmospheres.the effect of packaging of fresh sea bream fillets under different modified atmospheres (high levels of co2 combined with n2) on the behaviour of listeria monocytogenes and salmonella enteritidis was studied. two different strains of each pathogen were spread over the sea bream fillets stored at either 0 °c or 4 °c for 16 days under standard supermarket lighting conditions (14 h a day). the atmospheres investigated were 60% co2/40% n2, 70% co2/30% n2, 80% co2/20% n2 and air as control. mesophili ...201323454810
characterizing uncertainty when evaluating risk management metrics: risk assessment modeling of listeria monocytogenes contamination in ready-to-eat deli meats.this report illustrates how the uncertainty about food safety metrics may influence the selection of a performance objective (po). to accomplish this goal, we developed a model concerning listeria monocytogenes in ready-to-eat (rte) deli meats. this application used a second order monte carlo model that simulates l. monocytogenes concentrations through a series of steps: the food-processing establishment, transport, retail, the consumer's home and consumption. the model accounted for growth inhi ...201323454818
ruminant rhombencephalitis-associated listeria monocytogenes strains constitute a genetically homogeneous group related to human outbreak strains.listeriosis is a disease that causes significant economic losses at the farm level because of high morbidity and mortality in ruminants. this study was performed to investigate the role of ruminants in the epidemiology of listeriosis in northern italy and the possible association of animal-adapted strains of listeria monocytogenes with strains associated with human disease. twenty ruminant rhombencephalitis isolates previously confirmed as l. monocytogenes by bacteriology and pcr were characteri ...201323455337
different assembly of acid and salt tolerance response in two dairy listeria monocytogenes wild strains.a lack on the association between acid tolerance response (atr) and osmotolerance response (otr) among listeria monocytogenes dairy isolates was found. in order to evaluate how wild l. monocytogenes isolates mount tolerance responses under a sub-lethal ph and a low sodium chloride concentration (ph 5.5 and 3.5 % [w/v] nacl), a proteomic approach was used. the atr and otr of two l. monocytogenes cheese dairy isolates (strain t8, serotype 4b and a9, serotype 1/2b or 3b) were determined. the proteo ...201323455450
[protective immune responses induced by a recombinant listeria monocytogenes delivering hpv16 e7].to investigate specific immune responses elicited by a recombinant listeria monocytogenes strain lm4 deltahly::e7 and assess protective effect in c57bl/6 mice.201223457800
listeria monocytogenes alters mast cell phenotype, mediator and osteopontin secretion in a listeriolysin-dependent manner.whilst mast cells participate in the immune defence against the intracellular bacterium listeria monocytogenes, there is conflicting evidence regarding the ability of l. monocytogenes to infect mast cells. it is known that the pore-forming toxin listeriolysin (llo) is important for mast cell activation, degranulation and the release of pro-inflammatory cytokines. mast cells, however, are a potential source of a wide range of cytokines, chemokines and other mediators including osteopontin, which ...201323460827
adaptation of listeria monocytogenes in a simulated cheese medium: effects on virulence using the galleria mellonella infection model.the aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese-based medium on the virulence potential of listeria monocytogenes strains isolated from cheese and dairy processing environment using the galleria mellonella model. four l. monocytogenes strains were exposed to a cheese-based medium in conditions of induction of an acid tolerance response and osmotolerance response (ph 5·5 and 3·5% w/v nacl) and injected in g. mellonella insects. the survival of insects ...201323461530
fda-irisk--a comparative risk assessment system for evaluating and ranking food-hazard pairs: case studies on microbial hazards.stakeholders in the system of food safety, in particular federal agencies, need evidence-based, transparent, and rigorous approaches to estimate and compare the risk of foodborne illness from microbial and chemical hazards and the public health impact of interventions. fda-irisk (referred to here as irisk), a web-based quantitative risk assessment system, was developed to meet this need. the modeling tool enables users to assess, compare, and rank the risks posed by multiple food-hazard pairs at ...201323462073
airborne microbial composition in a high-throughput poultry slaughtering facility.a high-throughput chicken slaughtering facility in beijing was systematically investigated for numbers of airborne microorganisms. samples were assessed for counts of aerobic bacteria, staphylococcus aureus, total coliforms, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus cereus, and salmonella. during a 4-month period (september to december 2011), samples were collected for 10 min three times daily (preproduction, production, and postproduction). samples were collecte ...201323462077
commercial thermal process for inactivating salmonella poona on surfaces of whole fresh cantaloupes.outbreaks of salmonellosis by salmonella poona and listeriosis by listeria monocytogenes have been associated with the consumption of cantaloupes. commercial washing processes for cantaloupes are limited in their ability to inactivate and/or remove this human pathogen. our objective was to develop a commercial-scale surface pasteurization process for enhancing microbiological safety of cantaloupes. whole cantaloupes, surface inoculated with salmonella poona rm 2350, were stored at 32°c for 24 h ...201323462078
frequency of salmonella and listeria monocytogenes in five commercial brands of chicken eggs using a combined method of enrichment and nested-pcr.eggs or egg-based foods, either raw or undercooked, have been identified as vehicles of salmonella outbreaks. the low numbers of salmonella organisms in eggs makes it difficult to detect them in frequency studies. the nested-pcr (n-pcr) technique shows more sensitivity and specificity than bacteriological culture methods (bcms). a preenrichment method followed by enrichment and n-pcr is a good alternative for the investigation of salmonella and listeria monocytogenes in eggs. a total of 2,650 ch ...201323462079
effect of pulsed light treatments on the growth and resistance behavior of listeria monocytogenes 10403s, listeria innocua, and escherichia coli atcc 25922 in a liquid substrate.pulsed light (pl) treatment can effectively inactivate a large proportion of contaminating bacteria on surfaces and in clear solutions. an important issue that needs to be investigated is whether repeated exposure to pl treatment causes any changes to the growth and resistance behavior of the bacteria surviving the treatment. to test this, three challenge microorganisms were used: listeria monocytogenes, listeria innocua, and escherichia coli. cells of the challenge bacteria were treated with ei ...201323462080
antimicrobial activity of nanodispersed thymol in tryptic soy broth.food safety is a continuing challenge for the food industry due to sporadic illness outbreaks caused by foodborne pathogens. plant essential oils have been studied extensively as natural antimicrobials to control foodborne pathogens. however, their hydrophobic nature makes application in foods difficult because of their low water solubility, adverse impact on sensory quality, and binding with food components, which can interfere with antimicrobial efficacy. the objective of this study was to cha ...201323462081
presence and biological activity of antibiotics used in fuel ethanol and corn co-product production.antibiotics are used in ethanol production to control bacteria from competing with yeast for nutrients during starch fermentation. however, there is no published scientific information on whether antibiotic residues are present in distillers grains (dg), co-products from ethanol production, or whether they retain their biological activity. therefore, the objectives of this study were to quantify concentrations of various antibiotic residues in dg and determine whether residues were biologically ...201323463564
antimicrobial efficacy of poly (dl-lactide-co-glycolide) (plga) nanoparticles with entrapped cinnamon bark extract against listeria monocytogenes and salmonella typhimurium.nanoencapsulation of active compounds using poly-(d,l-lactide-co-glycolide) (plga) is commonly used in the pharmaceutical industry for drug delivery and may have important applications in the food industry. control of growth of foodborne bacteria with the goals of reducing the number of foodborne illness outbreaks, assuring consumers a safer food supply remains a priority in the food industry. natural antimicrobials are an excellent way to eliminate pathogens without introducing chemical preserv ...201323464835
co-production of surfactin and a novel bacteriocin by bacillus subtilis subsp. subtilis h4 isolated from bikalga, an african alkaline hibiscus sabdariffa seed fermented condiment.bikalga is a hibiscus sabdariffa seed fermented condiment widely consumed in burkina faso and neighboring countries. the fermentation is dominated by bacillus subtilis group species. ten b. subtilis subsp. subtilis (six isolates) and bacillus licheniformis (four isolates) isolated from traditional bikalga were examined for their antimicrobial activity against a panel of 36 indicator organisms including gram-positive and gram-negative bacteria and yeasts. the bacillus spp. isolates showed variabl ...201323466466
successful vaccination of immune suppressed recipients using listeria vector hiv-1 vaccines in helminth infected mice.vaccines for hiv, malaria and tb remain high priorities, especially for sub-saharan populations. the question is: will vaccines currently in development for these diseases function in populations that have a high prevalence of helminth infection? infection with helminth parasites causes immune suppression and a cd4+ th2 skewing of the immune system, thereby impairing th1-type vaccine efficacy. in this study, we conduct hiv vaccine trials in mice with and without chronic helminth infection to mim ...201323470236
the central role of arginine catabolism in t-cell dysfunction and increased susceptibility to infection after physical injury.to explore the hypothesis that decreased arginine availability by myeloid-derived suppressor cells (mdscs) is a cause of t-cell dysfunction after physical injury (pi).201423470573
application of protective cultures against listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere.to control the growth, or reduce the numbers, of food pathogens such as listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere (map), the effectiveness of protective cultures was evaluated in this study. leuconostoc pseudomesenteroides pck18 reduced the counts of l. monocytogenes by 1.22 log cfu/g in chicken burgers under map after 24 d. furthermore, a reduction of 1.16 log cfu/g in c. jejuni together with a delay in the growth of lactic acid bacte ...201323472035
preservative action of 11s (glycinin) and 7s (β-conglycinin) soy globulin on bovine raw milk stored either at 4 or 25 °c.considerable inhibitory antibacterial actions were exerted by the soybean 11s subunit comparable with nisin on the proliferation of total viable count, pseudomonas count and enterobacteriaceae count in bovine milk stored at 4 or 25 °c for 30 d and 48 h, while 7s and lysozyme were much less effective. the maximum magnitudes of bacterial reduction by 11s and nisin were in the range 2-4 log cfu/ml. the proliferation of 3 pathogenic bacteria (listeria monocytogenes, salmonella enteritidis and escher ...201323473420
rapid and sensitive immunodetection of listeria monocytogenes in milk using a novel piezoelectric cantilever sensor.listeria monocytogenes (lm), an important food-borne pathogen that has a high mortality rate (≈ 30%), was successfully detected within an hour at the infection dose limit of 10(3)/ml, both in buffer and milk. lm detection was demonstrated using a novel asymmetrically anchored cantilever sensor and a commercially available antibody. sensor responses were confirmed using a secondary antibody binding step, similar to the sandwich elisa assays, as a means of signal amplification that also reduced th ...201323475140
[bacteriostatic and/or bactericidal extract of aloe vera gel on cultures of listeria monocytogenes].listeria monocytogenes is a bacteria responsible for food borne diseases (fbd). the effect of aloe vera gel extract as a possible bacteriostatic and/or bactericidal against listeria monocytogenes, was checked by determined the minimum inhibitory concentration (mic), the time of minimum inhibition (tmi) and minimum bactericidal concentration (mbc) solutions extract of aloe vera gel in different concentrations on cultures of listeria monocytogenes atcc 7635. we applied the agar diffusion method, u ...201223477211
a single point mutation in the listerial betl σ(a)-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity.betaine uptake in listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter betl. using a random mutagenesis approach, based on the e. coli xl1 red mutator strain, we identified a single point mutation in a putative promoter region upstream of the betl coding region which leads to a significant increase in betl transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress toleran ...201723478432
antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria.the increased resistance of pathogenic microorganisms is frequently attributed to the extreme and inadequate use of antibiotics and transmission of resistance within and between individuals. to counter the emergence of resistant microorganisms, considerable resources have been invested in the search for new antimicrobials. plants synthesize a diverse array of secondary metabolites (phytochemicals) known to be involved in defense mechanisms, and in the last few years it is recognized that some of ...201323480526
safety assessment and probiotic evaluation of enterococcus faecium yf5 isolated from sourdough.enterococcus faecium yf5, a strain previously isolated from sourdough, was assessed for safety and probiotic potential. its virulence and antibiotic resistant phenotypes (cytolysin and gelatinase production, antibiotic susceptibility) and genes (cyla, gele, ace, agg, esp, and vana) were surveyed. results indicated that the tested virulence determinants were nontoxic. in addition, e. faecium yf5 was sensitive to 3 antibiotics such as amoxicillin, vancomycin, and chloramphenicol. furthermore, resu ...201323488799
susceptibility of aged guinea pigs to repeated daily challenge with listeria monocytogenes.epidemiological data suggests that certain groups such as the elderly are more susceptible to listeriosis than the general population. repeated exposure to low levels of listeria monocytogenes may increase the probability of infection. increased susceptibility to infection in the elderly has been attributed in part to reduced activity of t cells. we investigated the impact of consecutive daily intragastric challenge with l. monocytogenes on the development of listeriosis within an aged guinea pi ...201323489051
isolation and identification of bacteriocinogenic strain of lactobacillus plantarum with potential beneficial properties from donkey milk.the goal of this study was to isolate and characterize a lactic acid bacteria (lab) from donkey milk with potential beneficial properties.201323489977
gene expression profiling of a nisin-sensitive listeria monocytogenes scott a ctsr deletion mutant.listeria monocytogenes is a food-borne pathogen of significant threat to public health. nisin is the only bacteriocin that can be used as a food preservative. due to its antimicrobial activity, it can be used to control l. monocytogenes in food; however, the antimicrobial mechanism of nisin activity against l. monocytogenes is not fully understood. the ctsr (class iii stress gene repressor) protein negatively regulates the expression of class iii heat shock genes. a spontaneous pressure-tolerant ...201323494707
lipid components and water soluble metabolites in salted and dried tuna (thunnus thynnus l.) roes.the salted and dried product of tuna roe (bottarga) is a seafood characteristic of the mediterranean area and exported all over the world. samples of bottarga from bluefin tunas (thunnus thynnus, l.) caught in the southwest mediterranean sea were analysed. the samples were characterised by high content of marine wax esters (55-67 mol% of lipid classes), of docosahexaenoic (22:6 n-3, 25 w%) and oleic (18:1 n-9, 19 w%) fatty acids. cholesterol was detected as 7-9 w% of lipids. free fatty acids, in ...201223497865
high content of biogenic amines in pecorino cheeses.pecorino refers to italian cheeses made exclusively from raw or pasteurized ewes' milk, characterized by a high content of fat matter and it is mainly produced in the middle and south of italy by traditional procedures. the autochthonous microbiota plays an important role in the organoleptic traits of pecorino cheese and it can influence biogenic amines (ba) content. the aim of this study was to characterize from microbiological and chemical point of view 12 randomly purchased commercial cheeses ...201223498190
fate of escherichia coli o157:h7, listeria monocytogenes, and salmonella on fresh-cut celery.illnesses from escherichia coli o157:h7, listeria monocytogenes, and salmonella have been associated with the consumption of numerous produce items. little is known about the effect of consumer handling practices on the fate of these pathogens on celery. the objective of this study was to determine pathogen behavior at different temperatures under different storage conditions. commercial fresh-cut celery was inoculated at ca. 3logcfu/g onto either freshly cut or outer uncut surfaces and stored i ...201223498192
growth kinetics of listeria monocytogenes and spoilage microorganisms in fresh-cut cantaloupe.the main objective of this study was to investigate the growth kinetics of listeria monocytogenes and background microorganisms in fresh-cut cantaloupe. fresh-cut cantaloupe samples, inoculated with three main serotypes (1/2a, 1/2b, and 4b) of l. monocytogenes, were incubated at different temperatures, ranging from 4 to 43 °c, to develop kinetic growth models. during storage studies, the population of both background microorganisms and l. monocytogenes began to increase almost immediately, with ...201223498195
cellular-flip, raji isoform (c-flip r) modulates cell death induction upon t-cell activation and infection.dysregulation of apoptosis caused by an imbalance of pro- and anti-apoptotic protein expression can lead to cancer, neurodegenerative, and autoimmune diseases. cellular-flip (c-flip) proteins inhibit apoptosis directly at the death-inducing signaling complex of death receptors, such as cd95, and have been linked to apoptosis regulation during immune responses. while the isoforms c-flipl and c-flips are well characterized, the function of c-flipr remains poorly understood. here, we demonstrate th ...201323505065
structural and dynamic features of cold-shock proteins of listeria monocytogenes, a psychrophilic bacterium.cold-shock proteins (csps), proteins expressed when the ambient temperature drops below the growth-supporting temperature, bind to single-stranded nucleic acids and act as rna chaperones to regulate translation. listeria monocytogenes is a psychrophilic food-borne pathogen that is problematic for the food industry. structures of csps from psychrophilic bacteria have not yet been studied. despite dramatic differences in the thermostability of csps of various thermophilic microorganisms, these pro ...201323506337
fas-mediated inflammatory response in listeria monocytogenes infection.the molecular mechanisms of fas (cd95/apo-1)-mediated apoptosis are increasingly understood. however, the role of fas-mediated production of proinflammatory cytokines such as il-18 and il-1β in bacterial infection is unclear. we demonstrate the importance of fas-mediated signaling in il-18/il-1β production postinfection with listeria monocytogenes without the contribution of caspase-1 inflammasome. il-18/il-1β production in l. monocytogenes-infected peritoneal exudate cells from fas-deficient mi ...201323509366
antibacterial and anti-inflammatory activities of mycelia of a medicinal mushroom from taiwan, taiwanofungus salmoneus (higher basidiomycetes).taiwanofungus salmoneus (t.t. chang et w.n. chou) sheng h. wu et al. (shiang-shan-chih), is a medicinal fungus indigenous to taiwan. the mycelium was prepared from submerged culture and its ethanolic and hot-water extracts were used to study its antibacterial and anti-inflammatory activities. gram-positive species (bacillus cereus, listeria monocytogenes, and staphylococcus aureus) and gram-negative species (escherichia coli and salmonella typhimurium) of bacteria were used. in addition to the i ...201323510283
the listeria monocytogenes chia chitinase enhances virulence through suppression of host innate immunity.environmental pathogens survive and replicate within the outside environment while maintaining the capacity to infect mammalian hosts. for some microorganisms, mammalian infection may be a relatively rare event. understanding how environmental pathogens retain their ability to cause disease may provide insight into environmental reservoirs of disease and emerging infections. listeria monocytogenes survives as a saprophyte in soil but is capable of causing serious invasive disease in susceptible ...201323512964
prevalence of campylobacter spp., salmonella spp., listeria monocytogenes, and hepatitis e virus in swine livers collected at an abattoir.we investigated the prevalence of campylobacter spp., salmonella spp., listeria monocytogenes, and hepatitis e virus (hev) in swine liver. we collected swine livers from 110 pigs at an abattoir from september 2011 to march 2012 [corrected] . pathogens were detected in the liver samples of 19 (17.3%) pigs. campylobacter spp. were isolated from the liver samples of 14 (12.7%) pigs. in 10 of the 14 campylobacter-positive pigs, bacteria were present in the internal regions of the liver. salmonella s ...201323514917
display of multimeric antimicrobial peptides on the escherichia coli cell surface and its application as whole-cell antibiotics.concerns over the increasing emergence of antibiotic-resistant pathogenic microorganisms due to the overuse of antibiotics and the lack of effective antibiotics for livestock have prompted efforts to develop alternatives to conventional antibiotics. antimicrobial peptides (amps) with a broad-spectrum activity and rapid killing, along with little opportunity for the development of resistance, represent one of the promising novel alternatives. their high production cost and cytotoxicity, however, ...201323516591
cross-recognition of aspergillus galactomannan caused by listeria monocytogenes infection.we report a case of listeria monocytogenes bacteremia in a leukemic patient having a positive assay for aspergillus galactomannan (gm), although no evidence of aspergillosis was found. supernatant obtained from l. monocytogenes strain suspension was reactive with gm-assay. l. monocytogenes produces a soluble antigen that is cross-reactive with aspergillus gm.201323518185
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