Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| 1h, 15n and 13c chemical shift assignment of the gram-positive conjugative transfer protein trahpip501. | conjugative transfer of dna represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. trah is a putative transfer protein of the type iv secretion system (t4ss) encoded by the gram-positive (g+) conjugative plasmid pip501. this molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. here, we report the near complete (1)h, (13)c and (15 ... | 2016 | 26559076 |
| characterization of wastewater effluents releasing from slaughterhouses in and around hyderabad city (india). | the present study was undertaken to determine the pollution load to the environment causing by the wastewater effluents releasing from organized and unorganized slaughterhouses in and around hyderabad city. the wastewater effluents collected from three slaughterhouses, at the sites of releasing out to the surrounding environment,were characterized in terms of physico-chemical and microbiological parameters. the physico-chemical parameters, such as temperature, ph, alkalinity, turbidity, total su ... | 2014 | 26563081 |
| enterococcus faecalis attenuates osteogenesis through activation of p38 and erk1/2 pathways in mc3t3-e1 cells. | to explore the role of enterococcus faecalis in the proliferation, apoptosis and differentiation of osteoblasts. | 2016 | 26572053 |
| antibacterial, antifungal and antioxidant activity of olea africana against pathogenic yeast and nosocomial pathogens. | olea africana leaves are used by bapedi people to treat different ailments. the use of these leaves is not validated, therefore the aim of this study is to validate antimicrobial properties of this plant. | 2015 | 26577343 |
| influence of biological fluids in bacterial viability on different hospital surfaces and fomites. | the hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. the present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. | 2016 | 26577628 |
| effect of antimicrobials used in regenerative endodontic procedures on 3-week-old enterococcus faecalis biofilm. | we evaluated the effect of various antimicrobials used in endodontic regeneration on a 3-week-old enterococcus faecalis biofilm. | 2016 | 26577870 |
| effect of a benzalkonium chloride surfactant-sodium hypochlorite combination on elimination of enterococcus faecalis. | the purpose of this investigation was to determine the effect of a sodium hypochlorite-surfactant combination on the removal of enterococcus faecalis from infected teeth. | 2016 | 26577873 |
| the impact of carvacrol on ammonia and biogenic amine production by common foodborne pathogens. | the impact of carvacrol at different levels (0.1%, 0.5%, and 1%) on ammonia (amn) and biogenic amines (bas) production by 8 common foodborne pathogens (fbps) (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila, and salmonella paratyphi a) was studied using a rapid high-performance liquid chromatography method. significant differences among bacteria (p < 0.05) in amn and ba production were obs ... | 2015 | 26580308 |
| study on in vivo effects of bacterial histidine kinase inhibitor, waldiomycin, in bacillus subtilis and staphylococcus aureus. | two-component signal transduction systems (tcss) represent one of the primary means by which bacteria sense and respond to changes in their environment, both intra- and extracellular. the highly conserved walk (histidine kinase)/walr (response regulator) tcs is essential for cell wall metabolism of low g+c gram-positive bacteria and acts as a master regulatory system in controlling and coordinating cell wall metabolism with cell division. waldiomycin, a walk inhibitor, has been discovered by scr ... | 2015 | 26582287 |
| graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant staphylococcus aureus. | methicillin-resistant staphylococcus aureus (mrsa) has been responsible for serious hospital infections worldwide. nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. in the past decade, graphene oxide (go) has emerged as a material that is often used to support and stabilize silver nanoparticles (agnps) for the preparation of novel antibacterial nanocomposites. in this work, we report the syn ... | 2015 | 26586946 |
| antibiotic resistance and biofilm formation of enterococcus faecalis in patient and environmental samples. | enterococci are opportunistic pathogens and are a major factor in nosocomial infections. they may contain ebp operon, which upon expression makes them highly prone to biofilm formation on biotic and abiotic surfaces. | 2015 | 26587208 |
| identification of enterococcus faecalis antigens specifically expressed in vivo. | molecular mechanism of the pathogenicity of enterococcus faecalis (e. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. here we report the identification of in vivo expressed antigens of e. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (cmat) and an experimental animal model of endodontic infection. | 2015 | 26587417 |
| establishment of a multi-species biofilm model to evaluate chlorhexidine efficacy. | chronic infections, for example, diabetic foot ulcers, have a large impact in terms of patient morbidity and mortality. these wounds are characterized by complex polymicrobial communities of bacteria, which may include a number of difficult-to-eradicate multidrug-resistant pathogens. | 2016 | 26597632 |
| biological changes of enterococcus faecalis in the viable but nonculturable state. | enterococcus faecalis may enter a viable but nonculturable (vbnc) state under adverse conditions. e. faecalis, the major bacterial species present in failed root canal treatments, is thought to survive after endodontic treatment by entering a vbnc state. in this study, we characterized the vbnc state of e. faecalis. we designed 3 different protocols to successfully induce the vbnc state. approximately one-third of bacteria entered a vbnc state after 15-30 days, and all remained viable for at lea ... | 2015 | 26600540 |
| in vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, lenzites quercina. | in recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. the phytochemistry as well as antimicrobial activity of extracts obtained from lenzites quercina was investigated. the extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. the quantity of phytochemicals found in the extra ... | 2015 | 26604928 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| infective endocarditis involving the pulmonary valve. | pulmonary valve (pv) infective endocarditis (ie) is a rare entity, accounting for 1.5% to 2% of cases of ie. published data are limited to a few case series and reports. we sought to review the mayo clinic experience and describe clinical, echocardiographic, and microbiologic features. we included all patients aged ≥18 years seen from 2000 to 2014 who had a diagnosis of native pv ie and unequivocal echocardiographic involvement of the pv. nine patients with pv ie were identified. isolated pv ie ... | 2015 | 26611123 |
| microbial adhesion on novel yttria-stabilized tetragonal zirconia (y-tzp) implant surfaces with nitrogen-doped hydrogenated amorphous carbon (a-c:h:n) coatings. | biomaterial surfaces are at high risk for initial microbial colonization, persistence, and concomitant infection. the rationale of this study was to assess the initial adhesion on novel implant surfaces of enterococcus faecalis, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and candida albicans upon incubation. | 2016 | 26612401 |
| synergistic antibacterial effect of the combination of ε-polylysine and nisin against enterococcus faecalis. | this study evaluated the antibacterial effect of the combination of ε-polylysine (ε-pl) and nisin against enterococcus faecalis strains. the combination of ε-pl and nisin showed synergistic antibacterial activity against three enterococcus strains. scanning electron microscopy and a membrane permeability assay revealed that the combined treatment with ε-pl and nisin synergistically damaged the cell morphology of e. faecalis strain r612z1 cells. both ε-pl and nisin can dissipate the transmembrane ... | 2015 | 26613915 |
| prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india. | today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ... | 2017 | 26617250 |
| virulence factors, antimicrobial resistance pattern and molecular analysis of enterococcal strains isolated from burn patients. | the enterococci are emerging as a significant cause of hospital acquired infections. the pathogenesis of enterococci is attributed to the production of virulence factors and resistance to antibiotics. the purpose of the study was to assess the prevalence of genes encoding virulence factor, antimicrobial resistance determinant and molecular characteristic of enterococci isolated from burn patients. a total of 57 enterococci isolated from wound specimens of patients with burn injury were character ... | 2016 | 26620079 |
| clinical and microbiological features of bacteremia caused by enterococcus faecalis. | enterococcus faecalis is a frequent etiologic agent of invasive infections in hospitalized patients. the aim of this study was to analyze clinical and microbiological features of bacteremia caused by e. faecalis. | 2015 | 26623628 |
| on-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis platform for the detection of viruses and bacteria. | this work presents an on-chip isothermal nucleic acid amplification test (inaat) for the multiplex amplification and detection of viral and bacterial dna by a flow-based chemiluminescence microarray. in a principle study, on-chip recombinase polymerase amplification (rpa) on defined spots of a dna microarray was used to spatially separate the amplification reaction of dna from two viruses (human adenovirus 41, phi x 174) and the bacterium enterococcus faecalis, which are relevant for water hygie ... | 2016 | 26624222 |
| novel structural components contribute to the high thermal stability of acyl carrier protein from enterococcus faecalis. | enterococcus faecalis is a gram-positive, commensal bacterium that lives in the gastrointestinal tracts of humans and other mammals. it causes severe infections because of high antibiotic resistance. e. faecalis can endure extremes of temperature and ph. acyl carrier protein (acp) is a key element in the biosynthesis of fatty acids responsible for acyl group shuttling and delivery. in this study, to understand the origin of high thermal stabilities of e. faecalis acp (ef-acp), its solution struc ... | 2015 | 26631734 |
| in vitro comparison of antimicrobial effectiveness of qmix and other final irrigants in human root canals. | final root canal irrigation stands as an effective strategy for eliminating the dentin infection. this study aimed to investigate and compare the antibacterial efficacy of qmix and other four final irrigation regimens in reducing enterococcus faecalis within human root canals. single-canal human teeth contaminated with e. faecalis for 4 weeks were prepared chemomechanically with sodium hypochlorite (naocl). then, the teeth were randomly assigned into six groups according to the final irrigation ... | 2015 | 26631907 |
| antibacterial and toxic effect of hydrogen peroxide combined with different concentrations of chlorhexidine in comparison with sodium hypochlorite. | hydrogen peroxide (h2o2) has been suggested to be used in sequence or in combination with chlorhexidine (chx) to enhance the antibacterial activity against enterococcus faecalis, but there is no research in the literature on the safety and effectiveness of this irrigation protocol. | 2015 | 26636124 |
| effect of gaseous ozone on enterococcus faecalis biofilm-an in vitro study. | the aim of this study was to evaluate the antimicrobial effect of gaseous ozone compared to conventional methods against enterococcus faecalis. | 2016 | 26637463 |
| bactericidal effect of er:yag laser combined with sodium hypochlorite irrigation against enterococcus faecalis deep inside dentinal tubules in experimentally infected root canals. | this study evaluated the bactericidal effect of er:yag laser radiation combined with sodium hypochlorite (naocl) irrigation in the treatment of enterococcus faecalis deep inside dentinal tubules. the er:yag laser was activated, respectively, at 0.3, 0.5 and 1.0 w for either 20 or 30 s; 52.5 g l(-1) naocl and normal saline were used for the control groups. root canals before and after treatments were examined using scanning electron microscopy (sem). bacterial reductions both on the root canal wa ... | 2016 | 26645354 |
| tricuspid valve endocarditis complicated by mobitz type ii heart block - a case report and literature review. | we present a case of a middle-aged male who manifested with low-grade fever and lower back pain. mri and bone scan of the spine were suggestive of vertebral osteomyelitis. blood cultures were persistently positive for enterococcus faecalis and echocardiogram revealed tricuspid valve endocarditis. there was no history of iv drug use and urine toxicology was negative. ekg showed mobitz type ii av block and a transesophageal echocardiogram revealed no valve ring or septal abscesses. the heart block ... | 2015 | 26653699 |
| comparative evaluation of the antimicrobial activity of different antimicrobial peptides against a range of pathogenic bacteria. | the rapid emergence of resistance to classical antibiotics has increased the interest in novel antimicrobial compounds. antimicrobial peptides (amps) represent an attractive alternative to classical antibiotics and a number of different studies have reported antimicrobial activity data of various amps, but there is only limited comparative data available. the mode of action for many amps is largely unknown even though several models have suggested that the lipopolysaccharides (lps) play a crucia ... | 2015 | 26656394 |
| anti-breast cancer effects of live, heat-killed and cytoplasmic fractions of enterococcus faecalis and staphylococcus hominis isolated from human breast milk. | development of tumour that is resistant to chemotherapeutics and synthetic drugs, coupled with their life-threatening side effects and the adverse effects of surgery and hormone therapies, led to increased research on probiotics' anticancer potentials. the current study investigated the potential of live, heat-killed cells (hkc) and the cytoplasmic fractions (cf) of enterococcus faecalis and staphylococcus hominis as anti-breast cancer agents. mcf-7 cell line was treated with 25, 50, 100 and 200 ... | 2016 | 26659392 |
| degree of bacterial microleakage at the implant-abutment junction in cone morse tapered implants under loaded and unloaded conditions. | different results have been reported on the internal colonization of cone morse connections under in vitro dynamic loading. the aim of the present in vitro study was to evaluate the bacterial leakage in cone morse implant-abutment connections, both under loaded and unloaded conditions. | 2015 | 26660625 |
| gram-positive cocci in dutch icus with and without selective decontamination of the oropharyngeal and digestive tract: a retrospective database analysis. | the objective of this study was to determine time trends in the rate of gram-positive cocci in 42 dutch icus that continuously used or did not use selective oropharyngeal decontamination (sod) or selective decontamination of the digestive tract (sdd) and icus that introduced sod/sdd. | 2016 | 26661393 |
| the opportunistic pathogen enterococcus faecalis resists phagosome acidification and autophagy to promote intracellular survival in macrophages. | while many strains of enterococcus faecalis have been reported to be capable of surviving within macrophages for extended periods, the exact mechanisms involved are largely unknown. in this study, we found that after phagocytosis by macrophages, enterococci-containing vacuoles resist acidification, and e. faecalis is resistant to low ph. ultrastructural examination of the enterococci-containing vacuole by transmission electron microscopy revealed a single membrane envelope, with no evidence of t ... | 2016 | 26663775 |
| hydrothermally treated chitosan hydrogel loaded with copper and zinc particles as a potential micronutrient-based antimicrobial feed additive. | large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrie ... | 2015 | 26664989 |
| reduction of viable enterococcus faecalis in human radicular dentin treated with 1% cetrimide and conventional intracanal medicaments. | the purpose of this study was to evaluate the viability of e. faecalis after a 14-day exposure to 1% cetrimide (cet), triantibiotic paste (i.e., metronidazole, minocycline, and ciprofloxacin), 2% chlorhexidine (chx) gel, and calcium hydroxide (ca[oh]2 ) in an infected dentine model. | 2016 | 26666992 |
| assessment of the antimicrobial activity of few saudi arabian snake venoms. | venoms of two cobras, four vipers, a standard antibiotic and an antimycotic, were evaluated comparatively, as antimicrobials. | 2015 | 26668657 |
| pyrrolocin a, a 3-decalinoyltetramic acid with selective biological activity, isolated from amazonian cultures of the novel endophyte diaporthales sp. e6927e. | natural products remain an important source of new therapeutics for emerging drug-resistant pathogens like candida albicans, which particularly affects immunocompromised patients. a bioactive 3-decalinoyltetramic acid, pyrrolocin a, was isolated from extracts of a novel amazonian fungal endophyte, e6927e, of the diaporthales family. the structure of the natural product was solved using nmr and cd spectroscopy and it is structurally related to the fungal setins, equisetin and phomasetin, which ar ... | 2015 | 26669095 |
| effects of salinity and temperature on inactivation and repair potential of enterococcus faecalis following medium- and low-pressure ultraviolet irradiation. | to investigate the medium-pressure (mp) and low-pressure (lp) ultraviolet (uv) susceptibility and the repair potential of enterococcus faecalis (dsm 20478) after uv treatment. | 2016 | 26669257 |
| anti-microbial efficacy of allium sativum extract against enterococcus faecalis biofilm and its penetration into the root dentin: an in vitro study. | sodium hypochlorite (naocl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study. | 2015 | 26672416 |
| an in vitro evaluation of antimicrobial activity of five herbal extracts and comparison of their activity with 2.5% sodium hypochlorite against enterococcus faecalis. | sodium hypochlorite is the most widely used irrigant in endodontic practice, but it has various disadvantages. literature has shown that herbal products such as propolis, azadirachta indica (ai), triphala, curcuma longa, and morinda citrifolia (mc) possess good antimicrobial properties and thus can be used as potential endodontic irrigants. | 2015 | 26672425 |
| antimicrobial and antifungal efficacy of spilanthes acmella as an intracanal medicament in comparison to calcium hydroxide: an in vitro study. | repeated endodontic failures are due to pathogens like enterococcus faecalis and candida albicans which are resistant to the common intracanal medicaments like calcium hydroxide (ca[oh]2). | 2015 | 26672426 |
| cleistochlamys kirkii chemical constituents: antibacterial activity and synergistic effects against resistant staphylococcus aureus strains. | cleistochlamys kirkii (benth) oliv., (annonaceae) is a medicinal plant traditionally used in mozambique to treat infectious diseases. | 2016 | 26674158 |
| the effect of addition of an eps degrading enzyme with and without detergent to 2% chlorhexidine on disruption of enterococcus faecalis biofilm: a confocal laser scanning microscopic study. | enterococcus faecalis is one of the most commonly occurring organisms retrieved from root canal treated teeth that show refractory apical periodontitis. though it is well known that the ability of e. faecalis to form a matrix-encased biofilm contributes to its pathogenicity, the role of extracellular dextran and dna in biofilm formation and its effect on the susceptibility of the biofilm to chlorhexidine remains poorly understood. it was hypothesized that the addition of an extracellular polymer ... | 2015 | 26675655 |
| substantivity of ag-ca-si mesoporous nanoparticles on dentin and its ability to inhibit enterococcus faecalis. | the main purpose of this study was to investigate the substantivity of ag-ca-si mesoporous nanoparticles (ag-mcsns) on dentin and its residual antibacterial effects against enterococcus faecalis. ag-mcsns were fabricated and characterized, ion release profile and ph were tested, and the ability to inhibit planktonic e. faecalis as well as the cytotoxicity was evaluated. dentin slices were medicated with ca(oh)2 paste, 2 % chlorhexidine gel and ag-mcsns paste for 7 days and then irrigated. dentin ... | 2016 | 26676862 |
| control of shigatoxin-producing escherichia coli in cheese by dairy bacterial strains. | bio-preservation could be a valuable way to control shigatoxin-producing escherichia coli (stec) in cheese. to this end, 41 strains were screened for their inhibitory potential on model cheese curd and on pasteurized and raw milk uncooked pressed cheeses. strains of lactococcus lactis, lactococcus garvieae, leuconostoc pseudomesenteroides, leuconostoc citreum, lactobacillus sp, carnobacterium mobile, enterococcus faecalis, enterococcus faecium, macrococcus caseolyticus and hafnia alvei reduced s ... | 2016 | 26678131 |
| microbial evolution of traditional mountain cheese and characterization of early fermentation cocci for selection of autochtonous dairy starter strains. | the microbial population of traditional mountain (tm) cheese was investigated and characterized for the selection of cocci suitable for developing new starter cultures. samples of milk, curd and cheese at different ripening times were enumerated in selective culture media and 640 colonies were isolated from curd and cheese after 24 h of ripening. the lactic acid bacteria (lab) isolated from m17 were clustered into 231 biotypes by rapd-pcr analysis and identified as lactococcus lactis, streptococ ... | 2016 | 26678135 |
| antimicrobial resistance and virulence genes in enterococci from wild game meat in spain. | a total of 55 enterococci (45 enterococcus faecium, 7 enterococcus faecalis, and three enterococcus durans) isolated from the meat of wild game animals (roe deer, boar, rabbit, pheasant, and pigeon) in north-western spain were tested for susceptibility to 14 antimicrobials by the disc diffusion method. all strains showed a multi-resistant phenotype (resistance to between three and 10 antimicrobials). the strains exhibited high percentages of resistance to erythromycin (89.1%), tetracycline (67.3 ... | 2016 | 26678143 |
| genomic sequence of a clinical vancomycin-resistant reference strain, enterococcus faecalis atcc 51299. | in this paper, we present a draft genome sequence of a quality control reference strain, enterococcus faecalis atcc 51299 (multilocus sequencing type [mlst] st6), which is sensitive to teicoplanin but resistant to vancomycin. it is used in an agar screening test for streptomycin, gentamicin, and vancomycin resistance and the resistance marker vanb. | 2015 | 26679597 |
| antimicrobial activity of aqueous and organic extracts of a saudi medicinal plant: rumex nervosus. | the antimicrobial effect of aerial part of rumex nervosus obtained from the southern region of saudi arabia was evaluated on bacterial strains staphylococcus aureus, methicillin resistant s. aureus, enterococcus faecalis, escherichia coli, pseudomonas aeruginosa, and fungal strain candida albicans. | 2017 | 26681888 |
| enterococcus faecalis septicemia and vertebral osteomyelitis after transrectal ultrasound guided biopsy of the prostate. | transrectal ultrasound guided prostate biopsy (trus) has rarely been associated with disseminated infection, yet the occurrence appears to be increasing. resistance to fluoroquinolones, the most commonly used prophylaxis, is one of the likely causes, with escherichia coli being the most commonly reported cause of these infections. herein we present what is, to our knowledge, the first case of enterococcus faecalis septicemia and vertebral osteomyelitis after trus. previously reported cases of th ... | 2015 | 26682075 |
| tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m). | tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ... | 2016 | 26682961 |
| the bactericidal effect of 2780 and 940 nm laser irradiation on enterococcus faecalis in bovine root dentin slices of different thicknesses. | the aim of this in vitro study was to investigate the antibacterial effect of the dual wavelength (2780 nm er,cr:ysgg and 940 nm diode) laser in elimination of enterococcus faecalis in comparison with a 2780 nm er,cr:ysgg laser alone. | 2016 | 26685038 |
| antimicrobial activity of aspilia latissima (asteraceae). | we evaluated the antimicrobial activity of aspilia latissima - an abundant plant from the brazilian pantanal region - against candida albicans, candida parapsilosis, candida krusei, candida tropicalis, pseudomonas aeruginosa, enterococcus faecalis, escherichia coli and staphylococcus aureus. the crude extracts and fractions showed activity in all tested microorganisms. the chloroform fraction of the leaves and roots showed the most antimicrobial activity against s. aureus, with an mic of 500 μg/ ... | 2017 | 26691468 |
| olive mill waste extracts: polyphenols content, antioxidant, and antimicrobial activities. | natural polyphenols extracts have been usually associated with great bioactive properties. in this work, we investigated in vitro antioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (owwe) and the olive cake extracts (oce). using the folin ciocalteux method, owwe contained higher total phenol content compared to oce (8.90 ± 0.728 g/l versus 0.95 ± 0.017 mg/g). the phenolic compounds identification was carried out with a performance liquid chromatograph coupled ... | 2015 | 26693221 |
| development of nitrocellulose membrane filters impregnated with different biosynthesized silver nanoparticles applied to water purification. | bactericidal water filters were developed. for this purpose, nitrocellulose membrane filters were impregnated with different biosynthesized silver nanoparticles. silver nanoparticles (agnps) from aspergillus niger (agnps-asp), cryptococcus laurentii (agnps-cry) and rhodotorula glutinis (agnps-rho) were used for impregnating nitrocellulose filters. the bactericidal properties of these nanoparticles against escherichia coli, enterococcus faecalis and pseudomona aeruginosa were successfully demonst ... | 2016 | 26695258 |
| an antimicrobial metabolite from bacillus sp.: significant activity against pathogenic bacteria including multidrug-resistant clinical strains. | in this study, the cell free modified tryptone soya broth (ph 7.4 ± 0.2) of bacillus subtilis urid 12.1 showed significant antimicrobial activity against multidrug-resistant strains of staphylococcus aureus, s. epidermidis, streptococcus pyogenes and enterococcus faecalis. the partially purified antimicrobial molecule was found to be resistant to extremes of ph and temperatures and also to higher concentrations of trypsin and proteinase k. the antimicrobial molecule was purified by a three-step ... | 2015 | 26696963 |
| the capability of tyramine production and correlation between phenotypic and genetic characteristics of enterococcus faecium and enterococcus faecalis strains. | the aim of this study was to investigate the diversity of tyramine production capability of four enterococcus strains in buffered systems in relation to their genetic characteristics and environmental conditions. cells of the strains enterococcus faecalis ef37 and atcc 29212, and e. faecium fc12 and fc643 were re-suspended in phosphate/citrate buffers with different ph, nacl concentration and incubation temperature. at intervals, cell viability and tyramine production were assessed by plate coun ... | 2015 | 26696981 |
| evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of enterococcus faecalis. | background and aims. triple antibiotic paste (tap) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. the aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of tap at 1-, 2-, 3-, and 4-week intervals on mature enterococcus faecalis biofilm. materials and methods. a total of 287 extracted one-rooted human central incisors were infected with e. faecalis atcc 29212 after removing the crown and preparation ... | 2015 | 26697145 |
| antimicrobial activity of protamine against oral microorganisms. | protamine is an arginine-rich polycationic protein extracted from sperm cells of vertebrates including fishes such as salmon. the purpose of this study was to investigate the suppressive effects of protamine on the growth of oral pathogens for possible usage in dental materials. minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined by the microdilution method. twelve strains of oral viridans streptococci, actinomyces naeslundii, actinomyces odontolyt ... | 2015 | 26699859 |
| [a case of pyoderma gangrenosum of the penis difficult to distinguish from fournier gangrene]. | here, we report a case of pyoderma gangrenosum of the penis which was difficult to distinguish from fournier gangrene. the patient was a 54-year-old male who was aware of redness and swelling of the glanspenis for 1 month prior to a consultation at our department. although he was diagnosed with herpes and treated at a nearby hospital, his symptoms did not improve. subsequently, the patient visited our department following the onset of pain and fever. during his initial consultation, he had a fev ... | 2015 | 26699892 |
| [preliminary analyses on bacterial diversity and resistance in infection-related skin disorders]. | to explore the bacterial diversity and resistance in infection-related skin disorders. | 2015 | 26704163 |
| human health risks associated with antimicrobial-resistant enterococci and staphylococcus aureus on poultry meat. | enterococci and staphylococci are frequent contaminants on poultry meat. enterococcus faecalis, enterococcus faecium and staphylococcus aureus are also well-known aetiological agents of a wide variety of infections resulting in major healthcare costs. this review provides an overview of the human health risks associated with the occurrence of these opportunistic human pathogens on poultry meat with particular focus on the risk of food-borne transmission of antimicrobial resistance. in the absenc ... | 2016 | 26706616 |
| synthesis and biological evaluation of 2-aminobenzothiazole and benzimidazole analogs based on the clathrodin structure. | a series of 2-aminobenzothiazole and benzimidazole analogs based on the clathrodin scaffold was synthesized and investigated for their antimicrobial and antiproliferative activities as well as for their effects in hepatitis c virus (hcv) replicon model. compound 7, derived from 2-aminobenzothiazole, exhibited moderate antimicrobial activity only against the gram-positive bacterium, enterococcus faecalis. in the antiviral assay, compounds 4d and 7 were found to suppress the hcv replicon by >70%, ... | 2016 | 26709468 |
| maldi-tof mass spectrometry: a powerful tool for clinical microbiology at hôpital principal de dakar, senegal (west africa). | our team in europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms). to evaluate the utility of maldi-tof ms in tropical africa in collaboration with local teams, we installed an apparatus in the hôpital principal de dakar (senegal), performed routine identification of isolates, and confirmed or completed their identification in france. in the case of discordance or a la ... | 2015 | 26716681 |
| discordance between perioperative antibiotic prophylaxis and wound infection cultures in patients undergoing pancreaticoduodenectomy. | wound infections after pancreaticoduodenectomy (pd) are common. the standard antibiotic prophylaxis given to prevent the infections is often a cephalosporin. however, this decision is rarely guided by microbiology data pertinent to pd, particularly in patients with biliary stents. | 2016 | 26720272 |
| first identification of tn916-like element in industrial strains of lactobacillus vini that spread the tet-m resistance gene. | the open process used to ferment sugar cane juice or molasses to produce ethanol fuel is prone to contamination by bacterial cells of different species, in particular lactobacilli. the situation can be exacerbated by the emergence of resistant cells to industrial antibiotics that are normally used to combat this contamination. in this work, two lactobacillus vini isolates from ethanol distilleries were identified and found to be resistant to doxycycline, a tetracycline derivative, although sensi ... | 2016 | 26722009 |
| effectiveness of edta and modified salt solution to detach and kill cells from enterococcus faecalis biofilm. | disruption of the matrix of endodontic biofilms will aid in their removal from a root canal. therefore, the aim of this study was to investigate the efficacy of edta and a modified salt solution (mss) to detach bacteria from biofilms. | 2016 | 26723483 |
| antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. | the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds. | 2016 | 26724423 |
| gentamicin induces efaa expression and biofilm formation in enterococcus faecalis. | enterococci have been ranked among the leading causes of nosocomial bacteremia and urinary tract infection. this study aimed to investigate the effect of ampicillin, vancomycin, gentamicin and ceftizoxime on biofilm formation and gene expression of colonization factors on enterococcus faecalis. twelve clinical isolates of e. faecalis were used to investigate the effect of antibiotics on biofilm formation and gene expression of efaa, asa1, ebpa, esp and ace. flow system assay and microtiter plate ... | 2016 | 26724739 |
| antibiotic susceptibility profiles of dairy leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix. | in spite of a global concern on the transfer of antibiotic resistances (ar) via the food chain, limited information exists on this issue in species of leuconostoc and weissella, adjunct cultures used as aroma producers in fermented foods. in this work, the minimum inhibitory concentration was determined for 16 antibiotics in 34 strains of dairy origin, belonging to leuconostoc mesenteroides (18), leuconostoc citreum (11), leuconostoc lactis (2), weissella hellenica (2), and leuconostoc carnosum ... | 2016 | 26726815 |
| characterization of bacterial isolates from the microbiota of mothers' breast milk and their infants. | this investigation assessed the potential of isolating novel probiotics from mothers and their infants. a subset of 21 isolates among 126 unique bacteria from breast milk and infant stools from 15 mother-infant pairs were examined for simulated gi transit survival, adherence to caco-2 cells, bacteriocin production, and lack of antibiotic resistance. of the 21 selected isolates a lactobacillus crispatus isolate and 3 lactobacillus gasseri isolates demonstrated good profiles of in vitro gi transit ... | 2015 | 26727418 |
| chromosomally and extrachromosomally mediated high-level gentamicin resistance in streptococcus agalactiae. | streptococcus agalactiae (group b streptococcus [gbs]) is a leading cause of sepsis in neonates. the rate of invasive gbs disease in nonpregnant adults also continues to climb. aminoglycosides alone have little or no effect on gbs, but synergistic killing with penicillin has been shown in vitro. high-level gentamicin resistance (hlgr) in gbs isolates, however, leads to the loss of a synergistic effect. we therefore performed a multicenter study to determine the frequency of hlgr gbs isolates and ... | 2016 | 26729498 |
| diversity and evolution of the tn5801-tet(m)-like integrative and conjugative elements among enterococcus, streptococcus, and staphylococcus. | this work describes the diversity and evolution of tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. we also examined 610 isolates of enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known ctn-tet(m) elements due to the scarcity of data about tn5801 among enterococci. genome location (by iceu-i-pulsed-field gel electrophoresis [pfge] hybridization/integration sit ... | 2016 | 26729505 |
| antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds. | black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively. | 2017 | 26729960 |
| in silico driven design and synthesis of rhodanine derivatives as novel antibacterials targeting the enoyl reductase inha. | here, we report on the design, synthesis, and biological evaluation of 4-thiazolidinone (rhodanine) derivatives targeting mycobacterial tuberculosis (mtb) trans-2-enoyl-acyl carrier protein reductase (inha). compounds having bulky aromatic substituents at position 5 and a tryptophan residue at position n-3 of the rhodanine ring were the most active against inha, with ic50 values ranging from 2.7 to 30 μm. the experimental data showed consistent correlations with computational studies. their anti ... | 2016 | 26730986 |
| enterococcus faecalis as multidrug resistance strains in clinical isolates in imam reza hospital in kermanshah, iran. | the current study aimed to investigate the prevalence of vancomycin-resistant enterococcus in e. faecalis and e. faecium and antimicrobial susceptibility patterns, then dominant genes responsible for vancomycin resistance were determined. for this propose, 180 clinical isolates of enterococcus were subjected for identification and antibiotic susceptibility assay. then, the gene responsible vancomycin resistant strains were determined. the results demonstrated the e. faecalis as a dominant entero ... | 2015 | 26738400 |
| synthesis, characterization and antimicrobial activities of novel silver(i) complexes with coumarin substituted n-heterocyclic carbene ligands. | eight new coumarin substituted silver(i) n-heterocyclic carbene (nhc) complexes were synthesized by the interaction of the corresponding imidazolium or benzimidazolium chlorides and ag2o in dichloromethane at room temperature. structures of these complexes were established on the basis of elemental analysis, (1)h nmr, (13)c nmr, ir and mass spectroscopic techniques. the antimicrobial activities of carbene precursors and silver nhc complexes were tested against standard strains: enterococcus faec ... | 2016 | 26740157 |
| early gut colonization of preterm infants: effect of enteral feeding tubes. | the aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates. | 2016 | 26741949 |
| asymptomatic bacteriuria in clinical urological practice: preoperative control of bacteriuria and management of recurrent uti. | asymptomatic bacteriuria (abu) is a common clinical condition that often leads to unnecessary antimicrobial use. the reduction of antibiotic overuse for abu is consequently an important issue for antimicrobial stewardship and to reduce the emergence of multidrug resistant strains. there are two issues in everyday urological practice that require special attention: the role of abu in pre-operative prophylaxis and in women affected by recurrent urinary tract infections (rutis). nowadays, this is t ... | 2016 | 26742080 |
| exogenous endophthalmitis caused by enterococcus casseliflavus: a case report and discussion regarding treatment of intraocular infection with vancomycin-resistant enterococci. | endophthalmitis caused by enterococci is rare, and cases involving vancomycin-resistant enterococci are even more so. due to the poor bioavailability of many antibiotics in the vitreous chamber, special considerations are required when choosing antibiotics to treat these infections. the authors report the first case of exogenous endophthalmitis caused by enterococcus casseliflavus via the unique mechanism of high-velocity water stream trauma from a toy water gun. a previously healthy four-year o ... | 2017 | 26744592 |
| antibacterial properties of tebipenem pivoxil tablet, a new oral carbapenem preparation against a variety of pathogenic bacteria in vitro and in vivo. | to systemically investigate the in vitro and in vivo antibacterial properties of tebipenem pivoxil tablet. in addition, acute toxicity of this preparation was also studied. | 2016 | 26751436 |
| antibacterial efficacy of azadirachta indica, mimusops elengi and 2% chx on multispecies dentinal biofilm. | to check the antimicrobial activity of azadirachta indica (neem), mimusops elengi (bakul), and chlorhexidine gluconate (chx) on multispecies biofilm of common endodontic pathogens such as streptococcus mutans, enterococcus faecalis, staphylococcus aureus and candida albicans. | 2017 | 26752840 |
| antimicrobial efficacy of different root canal sealers by using real-time polymerase chain reaction: an ex vivo study. | root canal sealers help to minimize leakage, provides antimicrobial activity to reduce the possibility of residual bacteria, and to resolve periapical lesion. | 2017 | 26752843 |
| antimicrobial effect of an oxazolidinone, lantibiotic and calcium hydroxide against enterococcus faecalis biofilm: an in vitro study. | the aim was to evaluate and compare the antimicrobial efficacy of an oxazolidinone (linezolid [lz]), lantibiotic (nisin), and calcium hydroxide against enterococcus faecalis biofilm formed on tooth substrate after 2 and 7 days. | 2017 | 26752879 |
| establishment and analysis of in vitro biomass from salvia corrugata vahl. and evaluation of antimicrobial activity. | demethylfruticuline a and fruticuline a, the most abundant compounds from the surface extract of salvia corrugata vahl., have shown antibacterial, antitumor and cytotoxic activities. in order to obtain these icetexane diterpenes from in vitro cultures of s. corrugata, protocols were developed for callus production, micropropagation and shoot regeneration. analysis of the regenerated shoots showed the presence of both icetexanes, micropropagated plants contained only fruticuline a, while the call ... | 2016 | 26753532 |
| the efficacy of photon-initiated photoacoustic streaming and sonic-activated irrigation combined with qmix solution or sodium hypochlorite against intracanal e. faecalis biofilm. | the aim of the study was to assess the antibacterial efficacy of photon-initiated photoacoustic streaming (pips) using an er:yag laser and sonic-activated irrigation combined with qmix irrigant or sodium hypochlorite against enterococcus faecalis intracanal biofilm. root canals of 91 human extracted single-canal teeth were instrumented, sterilized, contaminated with e. faecalis and incubated for 15 days. the infected teeth were then randomly distributed into six experimental groups: g1: pips/er: ... | 2016 | 26754179 |
| screening of bacteriocin-producing enterococcus faecalis strains for antagonistic activities against clostridium perfringens. | this study was conducted to isolate and characterize bacteriocin-producing bacteria against clostridium perfringens (c. perfringens) from domestic animals to determine their usefulness as probiotics. bacteriocin-producing bacteria were isolated from pig feces by the spot-on-lawn method. a total of 1,370 bacterial stains were isolated, and six were tentatively selected after identifying the inhibitory activity against the pathogenic indicator c. perfringens kctc 3269 and kctc 5100. the selected s ... | 2014 | 26761495 |
| comparison of culture, conventional and real-time pcr methods for listeria monocytogenes in foods. | we compared standard culture methods as well as conventional pcr and real-time pcr for the detection of listeria monocytogenes (l. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. no statistical differences were observed in sensitivity between the two selective media in all foods. in total, real-time pcr assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compare ... | 2014 | 26761501 |
| in vitro characterization of bacteriocin produced by lactic acid bacteria isolated from nem chua, a traditional vietnamese fermented pork. | the aim of this study was to screen and in vitro characterize the properties of bacteriocin produced by lactic acid bacteria isolated from vietnamese fermented pork (nem chua). one hundred and fifty lab were isolated from ten samples of nem chua and screened for bacteriocin-producing lactic acid bacteria. antimicrobial activity of bacteriocin was carried out by spot on lawn method against both gram positive and gram negative bacteria. one isolate, assigned as kl-1, produced bacteriocin and showe ... | 2015 | 26761868 |
| fumarate reductase-producing enterococci reduce methane production in rumen fermentation in vitro. | biotic agents such as fumarate-reducing bacteria can be used for controlling methane (ch4) production in the rumen. fumarate-reducing bacteria convert fumarate to succinate by fumarate reductase, ultimately leading to the production of propionate. fumarate-reducing bacteria in the genus enterococcus were isolated from rumen fluid samples from slaughtered korean native goats. the enterococci were identified as enterococcus faecalis srod5 and e. faecium srod by phylogenetic analyses of 16s rrna ge ... | 2016 | 26767574 |
| caenorhabditis elegans susceptibility to gut enterococcus faecalis infection is associated with fat metabolism and epithelial junction integrity. | gut bacteria-host interactions have been implicated in the pathogenesis of numerous human diseases, but few mechanisms have been described. the genetically tractable nematode worm caenorhabditis elegans can be infected with pathogenic bacteria, such as the human gut commensal enterococcus faecalis, via feeding, making it a good model for studying these interactions. | 2016 | 26769134 |
| complete genome sequence of enterococcus faecalis strain p8-1 isolated from wild magellanic penguin (spheniscus magellanicus) feces on the south coast of brazil. | enterococcus faecalis strains have a ubiquitous nature that allows them to survive in different niches. studies involving enterococci isolated from marine animals are scarce. therefore, in this study, we report the complete genome sequence of e. faecalis strain p8-1 isolated from feces of a magellanic penguin on the south coast of brazil. | 2016 | 26769928 |
| pathogen distribution and drug resistance in a burn ward: a three-year retrospective analysis of a single center in china. | to investigate the spread of multiple-resistant strain in a burn ward to inform clinical administration of antibiotic drugs, burn wound treatment and decision-making for infection control. a 3-year retrospective analysis was conducted. specimens from wounds, blood, catheter, sputum, urine and stool collected from inpatients of the second affiliated hospital of zhejiang university of medicine between january 1, 2011 and december 31, 2013 were cultured and strains were identified by automatic bact ... | 2015 | 26770555 |
| variability of cutaneous and nasal population levels between patients colonized and infected by multidrug-resistant bacteria in two brazilian intensive care units. | to compare cutaneous and nasal population levels between patients colonized and infected by multidrug-resistant organisms in two intensive care units. | 2015 | 26770762 |
| role of candida albicans secreted aspartyl protease sap9 in interkingdom biofilm formation. | the fungus candida albicans colonizes oral cavity surfaces and is carried by up to 60% of human populations. biofilm development by c. albicans may be modulated by oral streptococci, such as streptococcus gordonii, s. oralis or s. mutans, so as to augment pathogenicity. in this study we sought to determine if the cell wall-associated secreted aspartyl proteinase sap9 was necessary for hyphal adhesin functions associated with biofilm community development. a sap9δ mutant of c. albicans sc5314 for ... | 2016 | 26772652 |
| antibacterial activity and immunomodulatory effects on a bovine mammary epithelial cell line exerted by nisin a-producing lactococcus lactis strains. | twenty-nine strains of mastitis pathogens were used to study the antibacterial activity of the cell-free supernatants (cfs) of 25 strains of lactococcus lactis ssp. lactis. out of the tested strains, only the cfs of l. lactis ll11 and sl153 were active, inhibiting and killing most of the pathogens. by means of ultra-performance liquid chromatography/high resolution mass spectrometry, they were shown to produce nisin a, a class i bacteriocin. a variable sensitivity to nisin a-containing cfs was o ... | 2016 | 26774727 |
| laryngoscopes: evaluation of microbial load of blades. | laryngoscope blades were analyzed, and the presence of blood, bodily fluids, and microorganisms was verified, indicating their potential as a source of cross contamination during clinical usage. the way in which the blades are cleaned and disinfected in daily practice may place the patient and health care team at risk. the aim of this study was to determine the bacterial and fungal load on this equipment. | 2016 | 26775934 |
| prevalence, outcome and risk factor associated with vancomycin-resistant enterococcus faecalis and enterococcus faecium at a tertiary care hospital in northern india. | to determine the prevalence, genotype, risk factors and mortality in patients having vancomycin-resistant enterococcus faecalis (vr e. faecalis) and enterococcus faecium (vr e. faecium) infection or colonisation. | 2016 | 26776117 |
| physiological and structural differences between enterococcus faecalis jh2-2 and mutant strains resistant to (p)-divercin rv41. | the aim of this study was to show the differences that could exist at the physiological and structural levels between enterococcus faecalis jh2-2 (wild type) and three mutant strains resistant to divercin rv41. these mutant strains were recently isolated and characterized for their intermediate resistance to recombinant dvnrv41; a subclass iia bacteriocin produced by escherichia coli. these mutant strains were named 35a1 (altered in gene coding phosphoesterase activity), 35h1 (altered in gene co ... | 2010 | 26781317 |