Publications

identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana fusarium wilt.	banana fusarium wilt (also known as panama disease) is one of the most disastrous plant diseases. effective control methods are still under exploring. the endophytic bacterial strain itbb b5-1 was isolated from the rubber tree, and identified as serratia marcescens by morphological, biochemical, and phylogenetic analyses. this strain exhibited a high potential for biological control against the banana fusarium disease. visual agar plate assay showed that itbb b5-1 restricted the mycelial growth ...	2015	26133557
thermodynamic relationships with processivity in serratia marcescens family 18 chitinases.	the enzymatic degradation of recalcitrant polysaccharides is accomplished by synergistic enzyme cocktails of glycoside hydrolases (ghs) and accessory enzymes. many ghs are processive which means that they remain attached to the substrate in between subsequent hydrolytic reactions. chitinases are ghs that catalyze the hydrolysis of chitin (β-1,4-linked n-acetylglucosamine). previously, a relationship between active site topology and processivity has been suggested while recent computational effor ...	2015	26154587
risk assessment for the spread of serratia marcescens within dental-unit waterline systems using vermamoeba vermiformis.	vermamoeba vermiformis is associated with the biofilm ecology of dental-unit waterlines (duwls). this study investigated whether v. vermiformis is able to act as a vector for potentially pathogenic bacteria and so aid their dispersal within duwl systems. clinical dental water was initially examined for legionella species by inoculating it onto legionella selective-medium plates. the molecular identity/profile of the glassy colonies obtained indicated none of these isolates were legionella specie ...	2015	26159777
severe osteomyelitis and septic arthritis due to serratia marcescens in an immunocompetent patient.	septic arthritis and osteomyelitis due to serratia marcescens in immunocompetent patients without risk factors are extremely rare. here, we report a case of septic arthritis and severe adjacent osteomyelitis of the tibia due to serratia marcescens in a diabetic community-dweller patient. the patient had no contact with healthcare workers or facilities and had no chronic disease except for poorly controlled diabetes. without predisposing risk factors, this type of infection is extremely rare, eve ...	2015	26161276
ceftazidime-avibactam activity when tested against ceftazidime-nonsusceptible citrobacter spp., enterobacter spp., serratia marcescens, and pseudomonas aeruginosa from unites states medical centers (2011-2014).	a total of 6910 enterobacteriaceae with inducible ampc β-lactamases and 5328 pseudomonas aeruginosa were collected from 71 us hospitals in 2011-2014 and were susceptibility tested using the reference broth microdilution method. ceftazidime-avibactam demonstrated potent in vitro activity against all 3 enterobacteriaceae genus groups evaluated, with mic(50)/mic(90) values of 0.12/0.5 μg/ml for enterobacter spp. and serratia marcescens and 0.12/0.25 μg/ml for citrobacter spp. (99.8-99.9% susceptibi ...	2015	26162518
response of the insect immune system to three different immune challenges.	insects rely on an innate immune system to effectively respond to pathogenic challenges. most studies on the insect immune system describe changes in only one or two immune parameters following a single immune challenge. in addition, a variety of insect models, often at different developmental stages, have been used, making it difficult to compare results across studies. in this study, we used adult male acheta domesticus crickets to characterize the response of the insect innate immune system t ...	2015	26164746
surveillance of carbapenem non-susceptible gram negative strains and characterization of carbapenemases of classes a, b and d in a lebanese hospital.	the production of carbapenem-hydrolyzing enzymes has been recognized as one of the most currently relevant resistance mechanisms in gram negative bacterial isolates, and is being detected in various countries. in lebanon, carbapenem resistance was studied among gram negative pathogens collected from a university hospital from january to june of years 2011 and 2012. all isolates were subjected to phenotypic tests including antibiotic susceptibility, cloxacillin effect, modified hodge test, and et ...	2015	26164974
molecular cloning and characterization of gloverin from the diamondback moth, plutella xylostella l. and its interaction with bacterial membrane.	gloverin restricted to lepidoptera is known to be a glycine-rich and heat stable antimicrobial protein. the current research reports a 650 bp full-length cdna encoding gloverin from plutella xylostella (pxglo) by reverse transcription polymerase chain reaction and rapid amplification of cdna ends. pxglo transcript was detected in both developmental stages and several tissues of 4th instar naïve larvae of p. xylostella with higher levels in the fat bodies. the mrna levels of pxglo increased appre ...	2015	26178746
compounds in a particular production lot of tryptic soy broth inhibit staphylococcus aureus cell growth.	staphylococcus aureus newman strain and several methicillin-resistant s. aureus (mrsa) clinical isolates were grown on agar plates prepared with conventional lots of tryptic soy broth (tsb). cell growth of these strains was inhibited on agar plates containing tsb of a particular product lot (lot a), whereas the cell growth of s. aureus rn4220 strain and several other mrsa clinical isolates was not inhibited. the cell growth of a strain of s. epidermidis was also inhibited on agar plates containi ...	2015	26193939
laboratory replication of filtration procedures associated with serratia marcescens bloodstream infections in patients receiving compounded amino acid solutions.	specific deviations from united states pharmacopeia standards were analyzed to investigate the factors allowing an outbreak of serratia marcescens bloodstream infections in patients receiving compounded amino acid solutions.	2015	26195654
an unusual cause of ventriculoperitoneal shunt infection.	infection associated with ventriculoperitoneal (vp) shunt implantation can be a significant problem. vp shunt infection with serratia marcescens, a gram-negative anaerobic rod, usually is related to underlying abdominal disease. this article describes treatment of two patients suffering from a vp shunt infection with s. marcescens without underlying abdominal disease.	2015	26208014
cutaneous serratia marcescens infections in korea: a retrospective analysis of 13 patients.	serratia marcescens is a gram-negative bacillus belonging to the enterobacteriaceae family. because of increasing reports of antimicrobial resistance, this bacterium has received considerable attention and has emerged as an important pathogen. in order to reveal clinical and microbiological characteristics of s. marcescens cutaneous infection and to suggest appropriate antibiotic treatment, we retrospectively analyzed 17 strains isolated from wound swabs of korean patients between november 2005 ...	2016	26241516
a case of pulmonary serratia marcescens granuloma radiologically mimicking metastatic malignancy and tuberculosis infection.	serratia marcescens is a saprophytic gram-negative bacillus capable of causing a wide range of infections. a 57-year-old female was admitted to our hospital for four weeks with community acquired pneumonia. a chest x-ray, six weeks after discharge, demonstrated multiple, bilateral 'cannon ball'-like opacities and mediastinal lymphadenopathy which were highly suspicious of disseminated malignancy or tuberculosis. the only symptom that this patient had was a productive cough. she had multiple comm ...	2015	26265740
mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action.	mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ...	2015	26267052
challenging synergistic activity of poplar-bacteria association for the cd phytostabilization.	the synergistic activity between plants and microorganisms may contribute to the implementation of proactive management strategies in the stabilization of contaminated sites, although heavy metals, such as cadmium (cd), are potentially toxic to them. the aim of this study was to evaluate the degree of tolerance to cd contamination (supplying twice 40 mg kg(-1) of cd) in poplar cuttings [clone i-214, p. × euramericana (dode) guinier] inoculated or not with two concentrations of serratia marcescen ...	2015	26268621
a novel 6'-n-aminoglycoside acetyltransferase, aac(6')-ial, from a clinical isolate of serratia marcescens.	serratia marcescens iomtu115 has a novel 6'-n-aminoglycoside acetyltransferase-encoding gene, aac(6')-ial. the encoded protein aac(6')-ial has 146 amino acids, with 91.8% identity to the amino acid sequence of aac(6')-ic in s. marcescens sm16 and 97.3% identity to the amino acid sequence of aac(6')-iap in s. marcescens ww4. the minimum inhibitory concentrations of aminoglycosides for escherichia coli expressing aac(6')-ial were similar to those for e. coli expressing aac(6')-ic or aac(6')-iap. t ...	2016	26270859
enhanced production of prodigiosin by serratia marcescens mo-1 using ram horn peptone.	this work addresses the production of prodigiosin from ram horn peptone (rhp) using mo-1, a local isolate in submerged culture. first, a novel gram-negative and rod-shaped bacterial strain, mo-1, was isolated from the body of the grasshopper (poecilemon tauricola ramme 1951), which was collected from pesticide-contaminated fields. sequence analysis of 16s rdna classified the microbe as serratia marcescens. the substrate utilization potential (biolog) and fatty acid methyl ester profile (fame) of ...	2015	26273284
within-host evolution decreases virulence in an opportunistic bacterial pathogen.	pathogens evolve in a close antagonistic relationship with their hosts. the conventional theory proposes that evolution of virulence is highly dependent on the efficiency of direct host-to-host transmission. many opportunistic pathogens, however, are not strictly dependent on the hosts due to their ability to reproduce in the free-living environment. therefore it is likely that conflicting selection pressures for growth and survival outside versus within the host, rather than transmission potent ...	2015	26282271
necrotizing fasciitis of the abdominal wall caused by serratia marcescens.	in this article, we present the first case of necrotizing fasciitis affecting the abdominal wall caused by serratia marcescens and share results of a focused review of s. marcescens induced necrotizing fasciitis. our patient underwent aorto-femoral bypass grafting for advanced peripheral vascular disease and presented 3 weeks postoperatively with pain, erythema and discharge from the incision site in the left lower abdominal wall and underwent multiple debridement of the affected area. pathology ...	2015	26294949
eepr mediates secreted-protein production, desiccation survival, and proliferation in a corneal infection model.	serratia marcescens is a soil- and water-derived bacterium that secretes several host-directed factors and causes hospital infections and community-acquired ocular infections. the putative two-component regulatory system composed of eepr and eeps regulates hemolysis and swarming motility through transcriptional control of the swrw gene and pigment production through control of the piga-pign operon. here, we identify and characterize a role for eepr in regulation of exoenzyme production, stress s ...	2015	26324535
draft genome sequence of a clinical isolate of serratia marcescens, strain ah0650_sm1.	serratia marcescens strain ah0650_sm1 is a clinical multidrug-resistant isolate from australia. here, we report its annotated draft genome comprising 20 contigs. we identified chromosomal antimicrobial resistance genes including a tet(41) variant, an aac(6')-ic variant, ampc, a metallo-beta-lactamase, and several putative multidrug efflux pumps, as well as a novel prophage.	2015	26337895
skin abscess due to serratia marcescens in an immunocompetent patient after receiving a tattoo.	the incidence of skin infections caused by serratia marcescens is extremely low and such infections are typically observed in immunocompromised patients. the clinical manifestations of these infections include cellulitis, abscesses, fluctuant nodules, or granulomatous lesions. infections caused by s. marcescens are very difficult to treat due to their resistance to many antibiotics, which often leads to specific and prolonged treatment. infections after receiving a tattoo are very rare and are c ...	2015	26356072
effects of temperature, ph and nacl content on in vitro putrescine and cadaverine production through the growth of serratia marcescens ccm 303.	the aim of this study was to evaluate the combined effect of temperature (10, 20 and 37°c), ph (4, 5, 6, 7 and 8), and nacl content (0, 1, 3, 4, 5 and 6% w/v) on the growth and putrescine and cadaverine production of serratia marcescens ccm 303 under model conditions. the decarboxylase activity of s. marcescens was monitored in broth after cultivation. the cultivation medium was enriched with selected amino acids (ornithine, arginine and lysine; 0.2% w/v each) serving as precursors of biogenic a ...	2015	26357890
regulation of the chitin degradation and utilization system by the chix small rna in serratia marcescens 2170.	serratia marcescens 2170 produces three different types of chitinases and chitin-binding protein cbp21. we found that transposon insertion into the 5' untranslated region (5' utr) of chipq-ctb led to defective chitinase and cbp21 production. chix small rna possessed the complementary sequence of the 5' utrs of the chipq-ctb and chir and repressed the expression of chip and chir. chix was detected in a medium containing glucose, glycerol, glcnac, and (glcnac)2, but the expression of both chip and ...	2016	26364670
putting on the brakes: bacterial impediment of wound healing.	the epithelium provides a crucial barrier to infection, and its integrity requires efficient wound healing. bacterial cells and secretomes from a subset of tested species of bacteria inhibited human and porcine corneal epithelial cell migration in vitro and ex vivo. secretomes from 95% of serratia marcescens, 71% of pseudomonas aeruginosa, 29% of staphylococcus aureus strains, and other bacterial species inhibited epithelial cell migration. migration of human foreskin fibroblasts was also inhibi ...	2015	26365869
bactericidal effects of triclosan in soap both in vitro and in vivo.	on december 2013, the us fda proposed a rule stating that manufacturers must provide data to demonstrate that antibacterial soap is more effective than plain soap or water. the objective of the present study was to examine the in vitro and in vivo bactericidal effect of triclosan (the most widely used antiseptic agent in soap) in soap.	2015	26374612
biochemical characterization and validation of a catalytic site of a highly thermostable ts2631 endolysin from the thermus scotoductus phage vb_tsc2631.	phage vb_tsc2631 infects the extremophilic bacterium thermus scotoductus mat2631 and uses the ts2631 endolysin for the release of its progeny. the ts2631 endolysin is the first endolysin from thermophilic bacteriophage with an experimentally validated catalytic site. in silico analysis and computational modelling of the ts2631 endolysin structure revealed a conserved zn2+ binding site (his30, tyr58, his131 and cys139) similar to zn2+ binding site of eukaryotic peptidoglycan recognition proteins ...	2015	26375388
interdomain contacts and the stability of serralysin protease from serratia marcescens.	the serralysin family of bacterial metalloproteases is associated with virulence in multiple modes of infection. these extracellular proteases are members of the repeats-in-toxin (rtx) family of toxins and virulence factors, which mediated virulence in e. coli, b. pertussis, and p. aeruginosa, as well as other animal and plant pathogens. the serralysin proteases are structurally dynamic and their folding is regulated by calcium binding to a c-terminal domain that defines the rtx family of protei ...	2015	26378460
visualization of the serratia type vi secretion system reveals unprovoked attacks and dynamic assembly.	the type vi secretion system (t6ss) is a bacterial nanomachine that fires toxic proteins into target cells. deployment of the t6ss represents an efficient and widespread means by which bacteria attack competitors or interact with host organisms and may be triggered by contact from an attacking neighbor cell as a defensive strategy. here, we use the opportunist pathogen serratia marcescens and functional fluorescent fusions of key components of the t6ss to observe different subassemblies of the m ...	2015	26387948
[transgenic expression of serratia marcescens native and mutant nucleases modulates tobacco mosaic virus resistance in nicotiana tabacum l].	extracellular serratia marcescens nuclease is an extremely active enzyme which non-specifically degrades rna and dna. its antiviral activity was previously shown both in animals and in plants when applied exogenously. transgenic tobacco plants (nicotiana tabacum l cv. sr1) expressing s. marcescens chimeric, mutant, and intracellular mutant nuclease gene variants were regenerated and challenged with tobacco mosaic virus. the transgenic plants exhibited a higher level of resistance to the virus in ...	2015	26410939
periodontal-disease-associated biofilm: a reservoir for pathogens of medical importance.	the ecological diversity of the periodontal microenvironment may provide suitable conditions for the colonization of species not usually considered members of the oral microbiota. in this investigation, we aimed to determine the prevalence and levels of pathogenic species of medical relevance in the microbiota of individuals with distinct periodontal clinical status. subgingival biofilm was obtained from patients with periodontal health (h, n = 81), gingivitis (g, n = 55), generalized aggressive ...	2016	26416306
the evolution of reduced antagonism--a role for host-parasite coevolution.	why do some host-parasite interactions become less antagonistic over evolutionary time? vertical transmission can select for reduced antagonism. vertical transmission also promotes coevolution between hosts and parasites. therefore, we hypothesized that coevolution itself may underlie transitions to reduced antagonism. to test the coevolution hypothesis, we selected for reduced antagonism between the host caenorhabditis elegans and its parasite serratia marcescens. this parasite is horizontally ...	2015	26420682
kpc-producing serratia marcescens in a home-care patient from recife, brazil.	in this brief communication we describe the occurrence of a kpc-producing serratia marcescens isolate in a home-care patient from recife, brazil. the blakpc, blaspm, blaimp, blavim, blaoxa, blactx-m, blashv, blatem and blages genes were investigated by polymerase chain reaction (pcr) and dna sequencing. the isolate was positive for blakpc-2 and blatem-1 and was resistant to aztreonam, cefepime, cefotaxime, imipenem, meropenem, gentamicin, ciprofloxacin and cefazidime, and susceptible only to ami ...	2017	26422164
efficient recombinant production of prodigiosin in pseudomonas putida.	serratia marcescens and several other bacteria produce the red-colored pigment prodigiosin which possesses bioactivities as an antimicrobial, anticancer, and immunosuppressive agent. therefore, there is a great interest to produce this natural compound. efforts aiming at its biotechnological production have so far largely focused on the original producer and opportunistic human pathogen s. marcescens. here, we demonstrate efficient prodigiosin production in the heterologous host pseudomonas puti ...	2015	26441905
biosynthesis of proline.	proline was among the last biosynthetic precursors to have its biosynthetic pathway unraveled. this review recapitulates the findings on the biosynthesis and transport of proline. glutamyl kinase (gk) catalyzes the atp-dependent phosphorylation of l-glutamic acid. purification of γ-gk from escherichia coli was facilitated by the expression of the prob and proa genes from a high-copy-number plasmid and the development of a specific coupled assay based on the nadph-dependent reduction of gp by γ-g ...	2007	26443591
serratiopeptidase: a well-known metalloprotease with a new non-proteolytic activity against s. aureus biofilm.	the use of indwelling medical devices is associated with a significant risk of infections by staphylococcus aureus (s. aureus) which possesses a variety of virulence factors including many toxins and the ability to invade eukaryotic cells or to form biofilm on biotic and abiotic surfaces. the virulence factors above described are often related to proteins exposed on the bacterial surface. blocking s. aureus colonization may reduce the incidence of invasive infectious diseases. previously reports ...	2015	26453184
aerobic mercury-resistant bacteria alter mercury speciation and retention in the tagus estuary (portugal).	aerobic mercury-resistant bacteria were isolated from the sediments of two highly mercury-polluted areas of the tagus estuary (barreiro and cala do norte) and one natural reserve area (alcochete) in order to test their capacity to transform mercury. bacterial species were identified using 16s rrna amplification and sequencing techniques and the results indicate the prevalence of bacillus sp. resistance patterns to mercurial compounds were established by the determination of minimal inhibitory co ...	2016	26461264
slow off-rates and strong product binding are required for processivity and efficient degradation of recalcitrant chitin by family 18 chitinases.	processive glycoside hydrolases are the key components of enzymatic machineries that decompose recalcitrant polysaccharides, such as chitin and cellulose. the intrinsic processivity (p(intr)) of cellulases has been shown to be governed by the rate constant of dissociation from polymer chain (koff). however, the reported koff values of cellulases are strongly dependent on the method used for their measurement. here, we developed a new method for determining koff, based on measuring the exchange r ...	2015	26468285
necrotizing fasciitis due to serratia marcescens: case report and review of the literature.	necrotizing fasciitis is a severe, life-threatening infection. serratia marcescens, a gram-negative bacterium, is an extremely rare cause of necrotizing fasciitis.	2016	26498285
the chitinase c gene pschic from pseudomonas sp. and its synergistic effects on larvicidal activity.	pseudomonas sp. strain txg6-1, a chitinolytic gram-negative bacterium, was isolated from a vegetable field in taixing city, jiangsu province, china. in this study, a pseudomonas chitinase c gene (pschic) was isolated from the chromosomal dna of this bacterium using a pair of specific primers. the pschic gene consisted of an open reading frame of 1443 nucleotides and encoded 480 amino acid residues with a calculated molecular mass of 51.66 kda. the deduced pschic amino acid sequence lacked a sign ...	2015	26500441
an independent evaluation of a novel peptide mimetic, brilacidin (pmx30063), for ocular anti-infective.	brilacidin (bri), a novel defensin mimetic, was evaluated as an ocular anti-infective.	2016	26501484
optical scatter patterns facilitate rapid differentiation of enterobacteriaceae on chromagar™ orientation medium.	enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as chromagar(tm) orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. here, we investigated the ability of the laser optical sensor, bardot (bacterial rapid detection usin ...	2015	26503189
processivity, substrate positioning, and binding: the role of polar residues in a family 18 glycoside hydrolase.	the enzymatic degradation of recalcitrant polysaccharides such as cellulose (β-1,4-linked glucose) and chitin (β-1,4-linked n-acetylglucosamine) by glycoside hydrolases (ghs) is of significant biological and economical importance. in nature, depolymerization is primarily accomplished by processive ghs, which remain attached to the substrate between subsequent hydrolytic reactions. recent computational efforts have suggested that the processive ability of a gh is directly linked to the ligand bin ...	2015	26503416
risk factors and microbiological features of patients hospitalized for microbial keratitis: a 10-year study in a referral center in taiwan.	we conducted a retrospective, cross-sectional study to analyze predisposing factors, clinical features, and microbiological characteristics of patients with microbial keratitis hospitalized over 10 years.the medical records of 558 patients who were diagnosed with microbial keratitis and admitted to chang gung memorial hospital (cgmh), a referral center in taiwan, from january 1, 2003 to december 31, 2012 were reviewed. demographics, predisposing factors, isolated organisms, treatment, and hospit ...	2015	26512612
chitin from penaeus merguiensis via microbial fermentation processing and antioxidant activity.	the ability of three proteases producing bacteria such as pseudomonas aeruginosa, serratia marcescens, and bacillus pumilus on the demineralization and deproteinzation efficiency of shrimp waste, for chitin extraction, was investigated. statistical analysis of data was showed a significant difference between the percentage of demineralization and deproteinization in different bacteria species (p<0.05). the highest deproteinization (74.76%) and demineralization rate (78.46%) was obtained with p. ...	2016	26519877
bacteria associated with amblyomma cajennense tick eggs.	ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ...	2015	26537602
secondary metabolites produced by marine streptomyces as antibiofilm and quorum-sensing inhibitor of uropathogen proteus mirabilis.	quorum-sensing regulates bacterial biofilm formation and virulence factors, thereby making it an interesting target for attenuating pathogens. in this study, we investigated anti-biofilm and anti-quorum-sensing compounds from secondary metabolites of halophiles marine streptomyces against urinary catheter biofilm forming proteus mirabilis without effect on growth viability. a total of 40 actinomycetes were isolated from samples collected from different places in iraq including marine sediments a ...	2016	26538254
[necrotizing soft tissue infection caused by serratia marcescens in a patient treated with tocilizumab].	we report herein on a case of community-acquired necrotizing soft tissue infection caused by serratia marcescens. the patient had been treated with prednisolone, tocilizumab and tacrolimus for rheumatoid arthritis. since gram staining of the tissue revealed gram negative rod bacteria, ceftriaxone and clindamycin were administered as empiric therapy. tissue culture revealed s. marcescens. ceftriaxone was continued according to the antibiotic sensitivity. she underwent debridement of necrotic tiss ...	2015	26548297
binary interactions of antagonistic bacteria with candida albicans under aerobic and anaerobic conditions.	we used both aerobic and anaerobic liquid co-cultures, prepared with luria bertani broth, to study the effect of bacteria on the survival of candida albicans in the external environment, away from an animal host. the bacteria were represented by aeromonas hydrophila, bacillus cereus, bacillus subtilis, clostridium, enterobacter, klebsiella pneumoniae, kluyvera ascorbata and serratia marcescens. under aerobic conditions, the yeast's growth was inhibited in the presence of bacterial growth; howeve ...	2016	26566932
in vitro synergistic effects of fisetin and norfloxacin against aquatic isolates of serratia marcescens.	serratia marcescens is a common pathogenic bacterium that can cause infections in both humans and animals. it can cause a range of diseases, from slight wound infections to life-threatening bacteraemia and pneumonia. the emergence of antimicrobial resistance has limited the treatment of the diseases caused by the bacterium to a great extent. consequently, there is an urgent need to develop novel antimicrobial strategies against this pathogen. synergistic strategy is a new approach to treat the i ...	2016	26567906
in vitro antimicrobial activity of a siderophore cephalosporin, s-649266, against enterobacteriaceae clinical isolates, including carbapenem-resistant strains.	s-649266 is a novel siderophore cephalosporin antibiotic with a catechol moiety on the 3-position side chain. two sets of clinical isolate collections were used to evaluate the antimicrobial activity of s-649266 against enterobacteriaceae. these sets included 617 global isolates collected between 2009 and 2011 and 233 β-lactamase-identified isolates, including 47 kpc-, 49 ndm-, 12 vim-, and 8 imp-producers. the mic90 values of s-649266 against the first set of escherichia coli, klebsiella pneumo ...	2016	26574013
chemical characterization and evaluation of antibacterial, antifungal, antimycobacterial, and cytotoxic activities of talinum paniculatum.	in this study, the bioactivity of talinum paniculatum was evaluated, a plant widely used in folk medicine. the extract from the t. paniculatum leaves (le) was obtained by percolation with ethanol-water and then subjecting it to liquid-liquid partitions, yielding hexane (hx), ethyl acetate (etoac), butanol (buoh), and aqueous (aq) fractions. screening for antimicrobial activity of the le and its fractions was evaluated in vitro through broth microdilution method, against thirteen pathogenic and n ...	2017	26603226
serratia marcescens: a rare cause of recurrent implantable cardioverter defibrillator site infection.	we present a unique case of a patient who experienced recurrent infections of his implantable cardioverter defibrillator (icd) site with the bacterium serratia marcescens. this report highlights the virulence of this bacterium, its resistance to antibiotic therapy, and its ability to remain latent for prolonged periods between episodes of sepsis. it also demonstrates the merits of reimplanting devices at different sites in the context of serratia marcescens infection.	2015	26605092
serratia marcescens osteomyelitis in cushing's disease.	we report a case of a 46-year-old man with fever, hypotension and arthralgias of the ankles and knees after brain surgery for a pituitary tumour causing cushing's disease. blood and urine cultures isolated serratia marcescens; antibiotic susceptibility testing showed sensitivity to piperacillin-tazobactan and ciprofloxacin. articular mri showed inflammation and necrosis of both knees and ankles, and left hip and right elbow (compatible with osteomyelitis). culture of an ankle abscess on the ankl ...	2015	26621903
metastatic serratia endophthalmitis associated with extravasation injury in a preterm neonate.	the authors report a case of a preterm neonate who presented with lid edema, corneal edema, and an inflammatory membrane with whitish exudates in the pupillary area, suggestive of endophthalmitis. there was also a cutaneous ulcer with an eschar on the right wrist at the site of extravasation associated with previous intravenous catheter. cultures from the ulcer and vitreous samples both grew serratia marcescens with identical antibiotic sensitivity and resistance patterns. the ocular infection w ...	2017	26622140
application of the biolog system for characterization of serratia marcescens ss marcescens isolated from onsite wastewater technology (oswt).	the scope of this study was to apply the biolog system to identify and characterize a serratia strain isolated from the surface of black plastic pieces which constitute the fluidized bed filter (onsite wastewater technology, oswt). the preliminary isolation of the strain was done in the medium with tetracycline at a 16 mg/l concentration. to characterize the isolated strain, the following biolog methods were applied: (1) ecoplates microplates for evaluation of physiological profiling, (2) gen ii ...	2015	26629795
y-linked variation for autosomal immune gene regulation has the potential to shape sexually dimorphic immunity.	sexually dimorphic phenotypes arise from the differential expression of male and female shared genes throughout the genome. unfortunately, the underlying molecular mechanisms by which dimorphic regulation manifests and evolves are unclear. recent work suggests that y-chromosomes may play an important role, given that drosophila melanogaster ys were shown to influence the regulation of hundreds of x and autosomal genes. for y-linked regulatory variation (yrv) to facilitate sexually dimorphic evol ...	2015	26631557
[outbreak due to serratia marcescens associated with intrinsic contamination of aqueous chlorhexidine].	serratia marcescens is a widely distributed gram-negative rod, often associated to nosocomial infections. some outbreaks linked to contaminated antiseptic solutions have been reported. in this study we report a nosocomial outbreak of surgical site infection and catheter insertion site infection due to s. marcescens. 33 patients with positive cultures were studied after an index case was identified. epidemiological, microbiological and molecular analysis demostrated an intrinsic contamination of ...	2015	26633107
draft whole-genome sequence of serratia marcescens strain rm66262, isolated from a patient with a urinary tract infection.	serratia marcescens strains are ubiquitous bacteria isolated from environmental niches and also constitute emergent nosocomial opportunistic pathogens. here, we report on the draft genome sequence of s. marcescens strain rm66262, which was isolated from a patient with urinary tract infection in the bacteriology service of the rosario national university, rosario, argentina.	2015	26634764
exploitation of a "hockey-puck" phenotype to identify pilus and biofilm regulators in serratia marcescens through genetic analysis.	pili are essential adhesive determinants for many bacterial pathogens. a suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative "hockey-puck" colony phenotype was designed to identify novel regulators of type i pili in serratia marcescens. mutations that decreased pilus biosynthesis mapped to the fimabcd operon; to the genes alat, fkpa, and oxyr; upstream of the flagellar master regulator operon flhdc; and to an uncharacterized gene encoding a predicted duf1401 doma ...	2016	26640000
molecular characterization by using next-generation sequencing of plasmids containing blandm-7 in enterobacteriaceae from calgary, canada.	enterobacteriaceae with blandm-7 are relatively uncommon and had previously been described in europe, india, the united states, and japan. this study describes the characteristics of enterobacteriaceae (klebsiella pneumoniae [n = 2], escherichia coli [n = 2], serratia marcescens [n = 1], and enterobacter hormaechei [n = 1] isolates) with blandm-7 obtained from 4 patients from calgary, canada, from 2013 to 2014. the 46,161-bp incx3 plasmids with blandm-7 are highly similar to other blandm-harbori ...	2016	26643346
multiyear, multinational survey of the incidence and global distribution of metallo-β-lactamase-producing enterobacteriaceae and pseudomonas aeruginosa.	metallo-β-lactamases (mbls) hydrolyze all classes of β-lactams except monobactams and are not inhibited by classic serine β-lactamase inhibitors. gram-negative pathogens isolated from patient infections were collected from 202 medical centers in 40 countries as part of a global surveillance study from 2012 to 2014. carbapenem-nonsusceptible enterobacteriaceae and pseudomonas aeruginosa were characterized for bla genes encoding vim, imp, ndm, spm, and gim variants using pcr and sequencing. a tota ...	2016	26643349
staphylococcal scalded skin syndrome in a premature newborn caused by methicillin-resistant staphylococcus aureus: case report.	staphylococcal scalded skin syndrome is an exfoliative skin disease. reports of this syndrome in newborns caused by methicillin-resistant staphylococcus aureus are rare but, when present, rapid diagnosis and treatment is required in order to decrease morbidity and mortality.	2015	26648436
swelling of poly(n-isopropylacrylamide) p(nipa)-based hydrogels with bacterial-synthesized prodigiosin for localized cancer drug delivery.	we present the results of swelling experiments on poly(n-isopropylacrylamide) p(nipa)-based hydrogels. the swelling characteristics of p(nipa)-based homo-polymer and p(nipa)-based co-polymers with acrylamide (am) and butyl methacrylate (bma), were studied using weight gain experiments. the swelling due to the uptake of biosynthesized cancer drug, prodigiosin (pg), was compared to swelling in controlled environments (distilled water (dw), paclitaxel™ (pt) and bromophenol blue (bb)). pg was synthe ...	2016	26652344
pore-forming toxins induce macrophage necroptosis during acute bacterial pneumonia.	necroptosis is a highly pro-inflammatory mode of cell death regulated by rip (or ripk)1 and rip3 kinases and mediated by the effector mlkl. we report that diverse bacterial pathogens that produce a pore-forming toxin (pft) induce necroptosis of macrophages and this can be blocked for protection against serratia marcescens hemorrhagic pneumonia. following challenge with s. marcescens, staphylococcus aureus, streptococcus pneumoniae, listeria monocytogenes, uropathogenic escherichia coli (upec), a ...	2015	26659062
antimicrobial susceptibility trends among gram-positive and -negative clinical isolates collected between 2005 and 2012 in mexico: results from the tigecycline evaluation and surveillance trial.	the tigecycline evaluation and surveillance trial (t.e.s.t) is a global antimicrobial surveillance study of both gram-positive and gram-negative organisms. this report presents data on antimicrobial susceptibility among organisms collected in mexico between 2005 and 2012 as part of t.e.s.t., and compares rates between 2005-2007 and 2008-2012.	2015	26667651
the effects of garlic-supplemented diets on skin mucosal immune responses, stress resistance and growth performance of the caspian roach (rutilus rutilus) fry.	this study was conducted to evaluate the effects of garlic supplementation on some skin mucus immune parameters, mucus antimicrobial activity and growth performance of the caspian roach (rutilus rutilus caspicus) fry. fish (1 ± 0.07 g) were divided into four groups fed diets containing 0 (control), 5, 10 and 15 g kg(-1) garlic for 8 weeks. the results showed that there was a significant increase in weight gain and specific growth rate in those fish fed garlic diets compared with the control (p < ...	2016	26700174
prevalence of contagious and environmental mastitis-causing bacteria in bulk tank milk and its relationships with milking practices of dairy cattle herds in são miguel island (azores).	this study aimed to assess the degree of contamination of bulk tank milk (btm) by staphylococcus spp. and coliform bacteria and to identify major milking practices that help perpetuate them in dairy cattle herds in são miguel island. in july 2014, btm was sampled and a survey concerning local milking practices was conducted on 100 herds. semi quantitative multiplex polymerase chain reaction detected coagulase-negative staphylococci, escherichia coli, staphylococcus aureus, and other coliform bac ...	2015	26719295
serratia marcescens septic sternoclavicular joint arthritis: a case report.		2016	26725022
the inhibitory effect of a lactobacillus acidophilus derived biosurfactant on biofilm producer serratia marcescens.	serratia marcescens is one of the nosocomial pathogen with the ability to form biofilm which is an important feature in the pathogenesis of s. marcescens. the aim of this study was to determine the anti-adhesive properties of a biosurfactant isolated from lactobacillus acidophilus atcc 4356, on s. marcescens strains.	2015	26730335
early gut colonization of preterm infants: effect of enteral feeding tubes.	the aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates.	2016	26741949
different food sources elicit fast changes to bacterial virulence.	environmentally transmitted, opportunistic bacterial pathogens have a life cycle that alternates between hosts and environmental reservoirs. resources are often scarce and fluctuating in the outside-host environment, whereas overcoming the host immune system could allow pathogens to establish a new, resource abundant and stable niche within the host. we tested if short-term exposure to different outside-host resource types and concentrations affect serratia marcescens-(bacterium)'s virulence in ...	2016	26763215
necrotizing soft tissue infection caused by serratia marcescens: a case report and literature review.	a 64-year-old man with advanced liver cirrhosis was transferred to an emergency center due to septic shock and markedly inflamed left leg. under a clinical diagnosis of necrotizing soft tissue infection (nsti), the patient undertook intensive therapy but died 25 h after arrival. the pathogenic organism, serratia marcescens, was later isolated from blood and soft tissue cultures. nsti is very rarely associated with s. marcescens. a literature review showed that only 16 such cases, including our c ...	2016	26778253
differences in the roles of the two surface-exposed tyrosine residues, y240 and y481, of serratia marcescens chitinase b during processive degradation of crystalline chitin.	chitinase b from serratia marcescens 2170 is one of the processive chitinases, and it has a linear path of aromatic amino acid residues on the surface and in the catalytic cleft. there are four surface-exposed residues lined-up towards the cleft, y481, w479, w252, and y240. the substitution of these residues with alanine causes a decrease in both the extent of the substrate binding and the hydrolytic activity (katouno et al., 2004). here, we examine the three mutants without losing the substrate ...	2015	26782656
pseudomonas putida strain fstm2 isolated from shark skin: a potential source of bacteriocin.	bacteriocin-producing pseudomonas putida strain fstm2 isolated from shark showed broad range of antibacterial activity against all pathogens tested except bacillus subtilis atcc11774, mrsa n32064, proteus mirabilis atcc12453, enterococcus faecalis atcc14506, salmonella typhimurium atcc51312, salmonella mutan atcc25175, and aeromonas hydrophila wbf314. of the three growth media tested in this study, tsb was observed to support the bacteriocin activity the most. while the highest bacteriocin activ ...	2013	26782985
a hospital-wide outbreak of serratia marcescens, and ishikawa's "fishbone" analysis to support outbreak control.	a nosocomial outbreak of serratia marcescens in respiratory samples predominantly from patients in a surgical intensive care unit is reported. most of these patients were cardiac surgical patients. initially, a vigorous but inconclusive investigation was implemented on the basis of standardized (according the us centers for disease control and prevention) steps of outbreak investigation. then, a systemic quality management approach with "fishbone" analysis was added. as a consequence, plausible ...	2016	26783861
serratia marcescens endogenous endophthalmitis in an immunocompetent host.	a systemically well 66-year-old white caucasian man presented to the urgent care department with a short history of progressive pain and blurring of vision in his left eye. he denied a history of trauma, intraocular surgery or use of illicit drugs. he was diagnosed with endogenous endophthalmitis. vitreous biopsy grew serratia marcescens, a gram negative bacteria. in spite of extensive investigation, there was no obvious source of infection. he had an indwelling urine catheter for prostate hyper ...	2016	26791115
complete genome sequence of serratia marcescens smunam836, a nonpigmented multidrug-resistant strain isolated from a mexican patient with obstructive pulmonary disease.	serratia marcescens smunam836 is a multidrug-resistant clinical strain isolated in mexico city from a patient with chronic obstructive pulmonary disease. its complete genome sequence was determined using pacbio rs ii smrt technology, consisting of a 5.2-mb chromosome and a 26.3-kb plasmid, encoding multiple resistance determinants and virulence factors.	2016	26798087
impact of azithromycin on the quorum sensing-controlled proteome of pseudomonas aeruginosa.	the macrolide antibiotic, azithromycin (azm), has been reported to improve the clinical outcome of cystic fibrosis patients, many of whom are chronically-infected with pseudomonas aeruginosa. however, the highest clinically-achievable concentrations of this drug are well-below the minimum inhibitory concentration for p. aeruginosa, raising the question of why azm exhibits therapeutic activity. one possibility that has been raised by earlier studies is that azm inhibits quorum sensing (qs) by p. ...	2016	26808156
spontaneous regression of tumour and the role of microbial infection--possibilities for cancer treatment.	this review deals with the role of microorganisms in spontaneous regression of a tumour. spontaneous cancer regression is a phenomenon that has been described for many centuries. one of the most well known methods of inducing spontaneous regression of cancer is the application of coley's toxin (heat-killed streptococcus pyogenes and serratia marcescens), which has been used for the successful treatment of sarcomas, carcinomas, lymphomas, myelomas and melanomas. in clinical practice, the use of b ...	2016	26813865
aromatic-mediated carbohydrate recognition in processive serratia marcescens chitinases.	microorganisms use a host of enzymes, including processive glycoside hydrolases, to deconstruct recalcitrant polysaccharides to sugars. processive glycoside hydrolases closely associate with polymer chains and repeatedly cleave glycosidic linkages without dissociating from the crystalline surface after each hydrolytic step; they are typically the most abundant enzymes in both natural secretomes and industrial cocktails by virtue of their significant hydrolytic potential. the ubiquity of aromatic ...	2016	26824449
conjugation is necessary for a bacterial plasmid to survive under protozoan predation.	horizontal gene transfer by conjugative plasmids plays a critical role in the evolution of antibiotic resistance. interactions between bacteria and other organisms can affect the persistence and spread of conjugative plasmids. here we show that protozoan predation increased the persistence and spread of the antibiotic resistance plasmid rp4 in populations of the opportunist bacterial pathogen serratia marcescens. a conjugation-defective mutant plasmid was unable to survive under predation, sugge ...	2016	26843557
augmentation of a microbial consortium for enhanced polylactide (pla) degradation.	bioplastics are eco-friendly and derived from renewable biomass sources. innovation in recycling methods will tackle some of the critical issues facing the acceptance of bioplastics. polylactic acid (pla) is the commonly used and well-studied bioplastic that is presumed to be biodegradable. considering their demand and use in near future, exploration for microbes capable of bioplastic degradation has high potential. four pla degrading strains were isolated and identified as penicillium chrysogen ...	2016	26843697
post-reconstitution stability of telavancin with commonly used diluents and intravenous infusion solutions.	the post-reconstitution chemical stability and microbial challenge hold time of nonpreserved telavancin for injection was determined using common reconstitution diluents and intravenous (iv) infusion solutions stored at room temperature with light (ambient) or at 2°c to 8°c without light (refrigeration).	2015	26843895
differences in the pharmacodynamics of ceftaroline against different species of enterobacteriaceae studied in an in vitro pharmacokinetic model of infection.	dose-ranging experiments were performed to study the pharmacodynamics of ceftaroline against enterobacteriaceae.	2016	26846209
[molecular analysis of an outbreak of serratia marcescens and its possible connection with the use of a chlorhexidine solution].		2016	26850716
osteomyelitis caused by veillonella species: case report and review of the literature.	previously, veillonella species had been considered as nonpathogenic and rarely caused serious infections. we report a case of 25-year-old man with osteomyelitis caused by veillonella species. he was admitted to the hospital due to an open fracture to the left radial bone caused by industrial washing machine accident, and emergency surgery was performed. however, wound infections occurred one week after the operation. although acinetobacter baumannii and serratia marcescens were cultured from th ...	2016	26857179
enhanced resistance to sclerotinia sclerotiorum in brassica napus by co-expression of defensin and chimeric chitinase genes.	sclerotinia stem rot caused by sclerotinia sclerotiorum is one of the major fungal diseases of brassica napus l. to develop resistance against this fungal disease, the defensin gene from raphanus sativus and chimeric chit42 from trichoderma atroviride with a c-terminal fused chitin-binding domain from serratia marcescens were co-expressed in canola via agrobacterium-mediated transformation. twenty transformants were confirmed to carry the two transgenes as detected by polymerase chain reaction ( ...	2016	26862081
shifting white pox aetiologies affecting acropora palmata in the florida keys, 1994-2014.	we propose 'the moving target hypothesis' to describe the aetiology of a contemporary coral disease that differs from that of its historical disease state. hitting the target with coral disease aetiology is a complex pursuit that requires understanding of host and environment, and may lack a single pathogen solution. white pox disease (wpx) affects the caribbean coral acropora palmata. acroporid serratiosis is a form of wpx for which the bacterial pathogen (serratia marcescens) has been establis ...	2016	26880837
emerging role of lipid droplets in aedes aegypti immune response against bacteria and dengue virus.	in mammals, lipid droplets (lds) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. in insects, it is unknown whether lds play any role during the development of immune responses. we show that aedes aegypti aag2 cells - an immune responsive cell lineage - accumulates lds when challenged with enterobacter cloacae, sindbis, and dengue viruses. microarray analysis of aag2 challenged with e.cloacae or infected with dengue virus reveal ...	2016	26887863
mechanisms of bacterial (serratia marcescens) attachment to, migration along, and killing of fungal hyphae.	we have found a remarkable capacity for the ubiquitous gram-negative rod bacterium serratia marcescens to migrate along and kill the mycelia of zygomycete molds. this migration was restricted to zygomycete molds and several basidiomycete species. no migration was seen on any molds of the phylum ascomycota. s. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well.s. marcescens did not exhibit growth tropism toward zygo ...	2016	26896140
i.v. minocycline revisited for infections caused by multidrug-resistant organisms.	the evidence supporting the potential use of i.v. minocycline for serious infections caused by multidrug-resistant organisms (mdros) is summarized.	2016	26896499
a six-month serratia marcescens outbreak in a neonatal intensive care unit.	to investigate a serratia marcescens (s. marcescens) outbreak in a neonatal unit in a tertiary university hospital.	2016	26900002
enhanced treatment of tannery wastewater in an integrated multistage bioreactor (imbr) by the predominant bacterial strains enriched from marine sediments.	an innovative integrated multistage bioreactor (imbr) system, which was augmented with three predominant bacterial strains (lactobacillus paracasei cl1107, pichia jadinii cl1705, and serratia marcescens cl1502) isolated from marine sediments, was developed to treat real tannery wastewater without performing physicochemical pretreatment, with the potential to reduce the generation of waste sludge and odors. the performance of the imbr treatment system, with and without the inclusion of the predom ...	2016	26901723
facilitation as attenuating of environmental stress among structured microbial populations.	there is currently an intense debate in microbial societies on whether evolution in complex communities is driven by competition or cooperation. since darwin, competition for scarce food resources has been considered the main ecological interaction shaping population dynamics and community structure both in vivo and in vitro. however, facilitation may be widespread across several animal and plant species. this could also be true in microbial strains growing under environmental stress. pure and m ...	2016	26904719
antibiotic resistance patterns of gram-negative psychrotrophic bacteria from bulk tank milk.	bacterial resistance to antibiotics is a major global health problem and resistance of pseudomonadaceae and enterobacteriaceae is a serious concern. we investigated the prevalence of drug-resistance in a total of 80 psychrotrophic strains from bulk milk belonging to pseudomonas genus (n. 63) and enterobacteriaceae group (n. 17). all the strains were tested against 16 antibiotics. pseudomonas were further investigated for their sensitivity against 12 additional antibiotics. pseudomonas showed a h ...	2016	26910385
antagonistic activity of nocardia brasiliensis ptcc 1422 against isolated enterobacteriaceae from urinary tract infections.	the main drawback of current antibiotic therapies is the emergence and rapid increase in antibiotic resistance. nocardiae are aerobic, gram-positive, catalase-positive, non-motile actinomycetes. nocardia brasiliensis was reported as antibiotic producer. the purpose of the study was to determine antibacterial activity of n. brasiliensis ptcc 1422 against isolated enterobacteriaceae from urinary tract infections (utis). the common bacteria from utis were isolated from hospital samples. antimicrobi ...	2016	26920557
interaction of prodigiosin with hsa and β-lg: spectroscopic and molecular docking studies.	human serum albumin (hsa) and bovine β-lactoglobulin (β-lg) are both introduced as blood and oral carrier scaffolds with high affinity for a wide range of pharmaceutical compounds. prodigiosin, a natural three pyrrolic compound produced by serratia marcescens, exhibits many pharmaceutical properties associated with health benefits. in the present study, the interaction of prodigiosin with hsa and β-lg was investigated using fluorescence spectroscopy, circular dichroism (cd) and computational doc ...	2016	26924214
impact of trypanosoma cruzi on antimicrobial peptide gene expression and activity in the fat body and midgut of rhodnius prolixus.	rhodnius prolixus is a major vector of trypanosoma cruzi, the causative agent of chagas disease in latin america. in natural habitats, these insects are in contact with a variety of bacteria, fungi, virus and parasites that they acquire from both their environments and the blood of their hosts. microorganism ingestion may trigger the synthesis of humoral immune factors, including antimicrobial peptides (amps). the objective of this study was to compare the expression levels of amps (defensins an ...	2016	26931761
intraperitoneal meropenem for peritoneal dialysis peritonitis with serratia marcescens immediately on commencing dialysis.	a 67-year-old man developed serratia marcescens peritonitis within a week of commencing peritoneal dialysis. dialysate cultures isolated multidrug-resistant s. marcescens, which was treated with intraperitoneal meropenem. this unusual case highlights the problem of multidrug-resistant peritoneal dialysis infections and the potential viability of intraperitoneal meropenem as ambulatory peritonitis therapy.	2016	26933500