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the combination of ampicillin plus ceftaroline is synergistic against enterococcus faecalis. 201525953804
in vitro comparison of the antibacterial effect of three intracanal irrigants and diode laser on root canals infected with enterococcus faecalis.bacteria are the primary etiology of pulpal and periradicular pathosis. in endodontically treated teeth with persistent infections only one or a few bacterial species are present of which the most important is enterococcus faecalis. the aim of this study was to compare antibacterial efficacy of canal disinfectants including 5.25% sodium hypochlorite, 2% chlorhexidine, mtad (a mixture of doxycycline, citric acid and a detergent (tween 80) and 830 nm diode laser.201425954488
surrogate testing suggests that chlorine dioxide gas exposure would not inactivate ebola virus contained in environmental blood contamination.the ability to decontaminate a room potentially containing the ebola virus is important to healthcare facilities in the united states. ebola virus remains viable in body fluids, a room that has housed a patient with ebola virus disease must have all surfaces manually wiped with an approved disinfectant, which increases occupational exposure risk. this study evaluated the efficacy of gaseous chlorine dioxide inactivation of bacterial organisms in blood as ebola virus surrogates and as the organis ...201525955403
daily variations in pathogenic bacterial populations in a monsoon influenced tropical environment.changing climatic conditions have influenced the monsoon pattern in recent years. variations in bacterial population in one such tropical environment were observed everyday over two years and point out intra and inter annual changes driven by the intensity of rainfall. vibrio spp. were abundant during the monsoon and so were faecal coliforms. vibrio alginolyticus were negatively influenced by nitrate, whereas, silicate and rainfall positively influenced vibrio parahaemolyticus numbers. it is als ...201525956443
enterocin f4-9, a novel o-linked glycosylated bacteriocin.enterococcus faecalis f4-9 isolated from egyptian salted-fermented fish produces a novel bacteriocin, termed enterocin f4-9. enterocin f4-9 was purified from the culture supernatant by three steps, and its molecular mass was determined to be 5,516.6 da by mass spectrometry. amino acid and dna sequencing showed that the propeptide consists of 67 amino acid residues, with a leader peptide containing a double glycine cleavage site to produce a 47-amino-acid mature peptide. enterocin f4-9 is modifie ...201525956765
small hospitals matter: insights from the emergence and spread of vancomycin-resistant enterococci in 2 public hospitals in inner brazil.although vancomycin-resistant enterococci (vre) are reported in brazil since 1996, data on their impact over settings of different complexity are scarce. we performed a study aimed at identifying determinants of vre emergence and spread in a public hospital consortium (comprising 2 hospitals, with 318 and 57 beds) in inner brazil. molecular typing and case-control studies (addressing predictors of acquisition or clonality) were performed. among 122 authocthonous isolates, 106 were enterococcus f ...201525956931
the effects of sodium hypochlorite and chlorhexidine irrigants on the antibacterial activities of alkaline media against enterococcus faecalis.sodium hypochlorite (naocl), chlorhexidine (chx) and calcium hydroxide are common intracanal medicaments. the present study aimed to evaluate the effects of naocl and chx on the antibacterial activities of alkaline media against enterococcus faecalis.201525956995
antibacterial properties associated with chitosan nanoparticle treatment on root dentin and 2 types of endodontic sealers.the aim of this study was to evaluate the efficacy of carboxymethyl chitosan (cmcs) and chitosan nanoparticles (cnps) to inactivate bacteria and prevent biofilm formation at sealer-dentin interfaces.201525958178
global in vitro activity of tigecycline and comparator agents: tigecycline evaluation and surveillance trial 2004-2013.the tigecycline evaluation and surveillance trial (test) is a global antimicrobial susceptibility surveillance study which has been ongoing since 2004. this report examines the in vitro activity of tigecycline and comparators against clinically important pathogens collected globally between 2004 and 2013.201525958201
an antibacterial ortho-quinone diterpenoid and its derivatives from caryopteris mongolica.to identify antibacterial components in traditional mongolian medicinal plant caryopteris mongolica, an ortho-quinone abietane caryopteron a (1) and three its derivatives caryopteron b-d (2-4) were isolated from the roots of the plant together with three known abietanes demethylcryptojaponol (5), 6α-hydroxydemethyl cryptojaponol (6), and 14-deoxycoleon u (7). the chemical structures of these abietane derivatives were elucidated on the basis of spectroscopic data. compounds 1-4 had c-13 methylcyc ...201525958242
synthesis and evaluation of cationic norbornanes as peptidomimetic antibacterial agents.a series of structurally amphiphilic biscationic norbornanes have been synthesised as rigidified, low molecular weight peptidomimetics of cationic antimicrobial peptides. a variety of charged hydrophilic functionalities were attached to the norbornane scaffold including aminium, guanidinium, imidazolium and pyridinium moieties. additionally, a range of hydrophobic groups of differing sizes were incorporated through an acetal linkage. the compounds were evaluated for antibacterial activity agains ...201525958967
apical extrusion of enterococcus faecalis using three different rotary instrumentation techniques: an in vitro study.to compare the apical extrusion of enterococcus faecalis after instrumentation with three different ni-ti rotary instruments- an in vitro study.201725961619
polyamine derivatives of betulinic acid and β-sitosterol: a comparative investigation.β-sitosterol and betulinic acid were used in designing their conjugates with selected polyamines bearing either an amide bond, or an ester and an amide bond simultaneously in the target molecule. the synthesized compounds were subjected to basic cytotoxic and antimicrobial tests. the synthetic protocol is described separately for each of the three series of the target amides, because each series of compounds required a different synthetic approach. the cytotoxicity was tested on cells derived fr ...201525963549
improved antibacterial phototoxicity of a neutral porphyrin in natural deep eutectic solvents.neutral porphyrins for antibacterial photodynamic therapy (apdt) have received little attention due to their tendency to aggregate in aqueous media and reports of low phototoxic effect. these compounds may be less toxic to cells than positively and negatively charged photosensitisers. the preparation of highly bacterial phototoxic formulations of neutral porphyrins remains an open field of research with great potential if achievable. the purpose of this study was to develop novel hydrophilic for ...201525966307
is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583.enterococcus faecalis is one of the most controversial species of lactic acid bacteria. some strains are used as probiotics, while others are associated with severe and life-threatening nosocomial infections. their pathogenicity depends on the acquisition of multidrug resistance and virulence factors. gelatinase, which is required in the first steps of biofilm formation, is an important virulence determinant involved in e. faecalis pathogenesis, including endocarditis and peritonitis. the gene t ...201525966618
which is the most effective disinfection method in primary root canals: conventional or newly developed ones?the aim of the study was to evaluate the antimicrobial activity of the-potassium-titanyl-phosphate--the ktp laser and ozone in of primary root canals.201525966729
antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits.the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ...201525973507
molecular characterization of resistance, virulence and clonality in vancomycin-resistant enterococcus faecium and enterococcus faecalis: a hospital-based study in beijing, china.the incidence of vancomycin-resistant enterococcus (vre) in china is increasing, the molecular epidemiology of vre in china is only partly known. this study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant enterococcus faecium (vrefm) and seven vancomycin-resistant enterococcus faecalis (vrefs) isolates obtained from a chinese hospital between july 2011 and july 2013. the glycopeptide resistance genes (vana and vanb) were sc ...201525976380
a novel gene, optra, that confers transferable resistance to oxazolidinones and phenicols and its presence in enterococcus faecalis and enterococcus faecium of human and animal origin.the oxazolidinone-resistant enterococcus faecalis e349 from a human patient tested negative for the cfr gene and 23s rrna mutations. here we report the identification of a novel oxazolidinone resistance gene, optra, and a first investigation of the extent to which this gene was present in e. faecalis and enterococcus faecium from humans and food-producing animals.201525977397
immunostimulatory effects triggered by enterococcus faecalis cect7121 probiotic strain involve activation of dendritic cells and interferon-gamma production.probiotics can modulate the immune system, conferring beneficial effects on the host. understanding how these microorganisms contribute to improve the health status is still a challenge. previously, we have demonstrated that enterococcus faecalis cect7121 implants itself and persists in the murine gastrointestinal tract, and enhances and skews the profile of cytokines towards the th1 phenotype in several biological models. given the importance of dendritic cells (dcs) in the orchestration of imm ...201525978357
the intraperitoneal transcriptome of the opportunistic pathogen enterococcus faecalis in mice.enterococcus faecalis is a gram-positive lactic acid intestinal opportunistic bacterium with virulence potential. for a better understanding of the adapation of this bacterium to the host conditions, we performed a transcriptome analysis of bacteria isolated from an infection site (mouse peritonitis) by rna-sequencing. we identified a total of 211 genes with significantly higher transcript levels and 157 repressed genes. our in vivo gene expression database reflects well the infection process si ...201525978463
the effect of benzalkonium chloride additions to ah plus sealer. antimicrobial, physical and chemical properties.the aim of this study was to determine the antimicrobial and antibiofilm activities and physicochemical properties of ah plus sealer mixed with different concentrations of benzalkonium chloride (bc).201525979825
antimicrobial residual effects of irrigation regimens with maleic acid in infected root canals.the success of endodontic treatment depends largely on the control of microorganisms present in infected root canals. the aim of this study was to determine the residual antimicrobial activity of several final irrigation protocols with 7% maleic acid (ma) alone and combined with chlorhexidine (chx), cetrimide (ctr) or both, in root canals infected with enterococcus faecalis. biofilms of e. faecalis were grown in uniradicular roots for 4 weeks. a total of 72 specimens were divided into 5 experime ...201525984506
decade-long use of the antimicrobial peptide combination tyrothricin does not pose a major risk of acquired resistance with gram-positive bacteria and candida spp.tyrothricin, an antimicrobial peptide combination produced by bacillus brevis consisting of gramicidins and tyrocidins commands broad antimicrobial activity against gram-positive bacteria and some yeasts in vitro. the polypeptide and its components have been used therapeutically for about 60 years in the local treatment of infected skin and infected oro-pharyngeal mucous membranes. though older studies suggest that resistance development of originally susceptible microorganisms towards tyrothric ...201425985581
infective endocarditis presenting as acute renal failure and unusual complications.infective endocarditis can be a diagnostic challenge. few cases have so far reported infective endocarditis presenting as renal failure. pseudoaneurysms of the mitral-aortic intervalvular fibrosa and splenic abscess are rare complications of infective endocarditis. we herein report a case of an 80-year-old man admitted due to anorexia, malaise, edema of the legs and renal failure. a progressive degradation of the patient's renal function was documented and hemodialysis was started. blood culture ...201525986267
a comparison of monomicrobial versus polymicrobial enterococcus faecalis bacteriuria in a french university hospital.enterococci are of considerable relevance in the hospital setting. their most common location is the urinary tract, where they may be responsible for both colonization and infections. they are often associated with the presence of other microorganisms. the aim was to compare monomicrobial and polymicrobial enterococcus faecalis bacteriuria. a retrospective study was performed on the demographic, clinical, and laboratory data of 299 patients who had presented with e. faecalis bacteriuria in 2012 ...201525987245
a derivative of the thiopeptide ge2270a highly selective against propionibacterium acnes.a chemical derivative of the thiopeptide ge2270a, designated nai003, was found to possess a substantially reduced antibacterial spectrum in comparison to the parent compound, being active against just a few gram-positive bacteria. in particular, nai003 retained low mics against all tested isolates of propionibacterium acnes and, to a lesser extent, against enterococcus faecalis. furthermore, nai003 showed a time- and dose-dependent killing of both a clindamycin-resistant and a clindamycin-sensit ...201525987631
polymicrobial q fever and enterococcal aortic prosthetic valve endocarditis with aortic root abscess.polymicrobial endocarditis is uncommon. we present a case of polymicrobial endocarditis caused by enterococcus faecalis and coxiella burnetii and review previous cases of polymicrobial endocarditis involving coxiella burnetii. testing for q fever should be considered in any patient with endocarditis living in an endemic area.201525988443
pharmacokinetics of enrofloxacin and ceftiofur in plasma, interstitial fluid, and gastrointestinal tract of calves after subcutaneous injection, and bactericidal impacts on representative enteric bacteria.this study's objectives were to determine intestinal antimicrobial concentrations in calves administered enrofloxacin or ceftiofur sodium subcutaneously, and their impact on representative enteric bacteria. ultrafiltration devices were implanted in the ileum and colon of 12 steers, which received either enrofloxacin or ceftiofur sodium. samples were collected over 48 h after drug administration for pharmacokinetic/pharmacodynamic analysis. enterococcus faecalis or salmonella enterica (5 × 10(5) ...201625989138
exploration of the mechanisms of biofilm formation by enterococcus faecalis in glucose starvation environments.enterococcus faecalis is the most common single species present in teeth after failed root canal therapy. this is mainly due to its ability to maintain viability for a long time in filled root canals where nutrients are normally sparse. the aim of this study was to explore the mechanism of e. faecalis survival and biofilm formation in glucose-starved environments.201525990488
bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms.the aim of the present study was to evaluate the bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400 (peg 400), and polyethylene glycol 1000 (peg 1000) against selected microorganisms in vitro.201725992336
in vitro effectiveness of brazilian brown propolis against enterococcus faecalis.the aim of this study was to evaluate the in vitro antimicrobial activity of brazilian brown propolis as an intracanal medication against enterococcus faecalis. thirty dentin discs prepared from intact freshly extracted bovine maxillary central incisors were infected with e. faecalis for 21 days. the specimens were distributed into six groups according to the medicament used as follows: g1- calcium hydroxide paste; g2- carbowax 400 (control group); g3- 20% brown propolis paste; g4- 40% brown pro ...201525992787
[bloodstream infections in southwestern china: 2012 whire union report on bacterial susceptibility to antibiotics].to test the susceptibility of blood culture isolates to antibiotics in a sample of hospitals in southwestern china in 2012.201525993745
obturation techniques allow microbial leakage unless protected.to evaluate the quality of the apical 5-mm seal produced by different filling techniques using a bacterial leakage model.201625996203
probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses.in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ...201525998823
composition and antimicrobial activity of euphrasia rostkoviana hayne essential oil.eyebright, euphrasia rostkoviana hayne (scrophulariaceae), is a medicinal plant traditionally used in europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. some euphrasia species have been previously reported to contain essential oil. however, the composition and bioactivity of e. rostkoviana oil are unknown. therefore, in this study, we investigated the chemical c ...201526000025
overexpression of enterococcus faecalis elr operon protects from phagocytosis.mechanisms underlying the transition from commensalism to virulence in enterococcus faecalis are not fully understood. we previously identified the enterococcal leucine-rich protein a (elra) as a virulence factor of e. faecalis. the elra gene is part of an operon that comprises four other orfs encoding putative surface proteins of unknown function.201526003173
the mazef toxin-antitoxin system as an attractive target in clinical isolates of enterococcus faecium and enterococcus faecalis.the toxin-antitoxin (ta) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. in this study, the prevalence of ta systems in independently isolated clinical isolates of enterococcus faecium and enterococcus faecalis was determined, the dominant ta system was identified, different virulence genes in e. faecium and e. faecalis were surveyed, the level of expression of the virulence and ta genes in normal and stress conditions was determined, and f ...201526005332
antimicrobial effect of zataria multiflora extract in comparison with chlorhexidine mouthwash on experimentally contaminated orthodontic elastomeric ligatures.long-term use of orthodontic appliances and fixation ligatures creates a favorable environment for the accumulation of oral normal microflora and increases the risk of enamel demineralization and periodontal disease. the aim of this study was to compare the antimicrobial effects of zataria multiflora extract and 0.2% chlorhexidine (chx) mouthwash on experimentally contaminated orthodontic elastomeric ligatures.201526005448
synthesis, structural characterization and biological activity of novel cyclohexane-1,3-dione ligands and their metal complexes.some new zn(ii) and cu(ii) complexes [cu(l1)(oac)2]∙h2o, [cu(l1)(no3)h2o]∙no3∙3.5h2o, [zn(l1)(no3)2]∙4.5h2o, [zn(l1)(oac)2(h2o)2]∙3h2o, [cu2(l2)(oac)4]∙2h2o∙2dmf, [cu(l2)2]∙2no3∙1.5dmf∙h2o, [zn(l2)2(no3)2]∙dmf and [zn2(l2)(oac)4(h2o)4]∙5h2o; l1 = 2-[2-(2-methoxyphenyl)hydrazono]cyclohexane-1,3-dione and l2 = 2-[2-(3-nitrophenyl)hydrazono]cyclohexane-1,3-dione were synthesized and characterized by ir, 1h-nmr,13c-nmr and ultraviolet (uv-vis.) spectroscopy, elemental analysis, magnetic susceptibili ...201526007185
the efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique.the aim of this in vitro study was to evaluate the efficacy of photodynamic therapy (pdt) and sodium hypochlorite (naocl) in root canal disinfection by a single-file instrumentation technique.201526007242
identification of enterococcus faecalis and pseudomonas aeruginosa on and in implants in individuals with peri-implant disease: a cross-sectional study.to verify whether the parts of dental implants can be contaminated by opportunistic pathogens.201526009909
collagen-like peptide sequences inhibit bacterial invasion of root dentine.to investigate the effects of peptides derived from the sequence of collagen to inhibit penetration of human or bovine dentine by species of streptococci and enterococci.201626011655
uses and limitations of green fluorescent protein as a viability marker in enterococcus faecalis: an observational investigation.enterococci are capable of producing biofilms that are notoriously difficult to treat and remove, for instance in root canal infections. the tenacious nature of these organisms makes screening of known and novel antimicrobial compounds necessary. while traditionally growth and fluorescence-based screening methods have proven useful, these methods have their limitations when applied to enterococci (e.g. time consuming, no kinetic data, diffusion properties of the fluorescent dyes). the aim of thi ...201526015063
correlation between enterococcus faecalis and persistent intraradicular infection compared with primary intraradicular infection: a systematic review.the relationship between enterococcus faecalis and pulpal or periradicular diseases has been studied for many years; however, whether e. faecalis is correlated with persistent intraradicular infections (teeth after failed endodontic treatments) compared with primary intraradicular infections remains controversial. the objective of this systematic review was to compare the prevalence of e. faecalis in primary and persistent intraradicular infections.201526015157
the enterococcus faecalis ebpa pilus protein: attenuation of expression, biofilm formation, and adherence to fibrinogen start with the rare initiation codon att.the endocarditis and biofilm-associated pili (ebp) are important in enterococcus faecalis pathogenesis, and the pilus tip, ebpa, has been shown to play a major role in pilus biogenesis, biofilm formation, and experimental infections. based on in silico analyses, we previously predicted that att is the ebpa translational start codon, not the atg codon, 120 bp downstream of att, which is annotated as the translational start. att is rarely used to initiate protein synthesis, leading to our hypothes ...201526015496
a novel termini analysis theory using hts data alone for the identification of enterococcus phage ef4-like genome termini.enterococcus faecalis and enterococcus faecium are typical enterococcal bacterial pathogens. antibiotic resistance means that the identification of novel e. faecalis and e. faecium phages against antibiotic-resistant enterococcus have an important impact on public health. in this study, the e. faecalis phage ime-ef4, e. faecium phage ime-efm1, and both their hosts were antibiotic resistant. to characterize the genome termini of these two phages, a termini analysis theory was developed to provide ...201526016565
antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants.phosphoric acid has been suggested as an irrigant due to its effectiveness in removing the smear layer.201526018307
analysis of vancomycin susceptibility testing results for presumptive categorization of telavancin.scattergrams between vancomycin and telavancin demonstrated susceptibility agreement rates of 99.96, 99.65, and 100.00% for staphylococcus aureus, enterococcus faecalis, and streptococci, respectively. a single very major error was obtained against e. faecalis, while vancomycin-intermediate s. aureus (visa) and vancomycin-resistant and teicoplanin-susceptible (vanb phenotype) e. faecalis were responsible for major and minor errors. these results support the use of vancomycin to infer telavancin ...201526019194
effectiveness of probiotic therapy for the prevention of relapse in patients with inactive ulcerative colitis.to evaluate the effectiveness of probiotic therapy for suppressing relapse in patients with inactive ulcerative colitis (uc).201526019464
statins and antimicrobial effects: simvastatin as a potential drug against staphylococcus aureus biofilm.statins are important lipid-lowering agents with other pleiotropic effects. several studies have explored a possible protective effect of statins to reduce the morbidity and mortality of many infectious diseases. staphylococcus aureus is one of the main pathogens implicated in nosocomial infections; its ability to form biofilms makes treatment difficult. the present study observed the mic of atorvastatin, pravastatin and simvastatin against s. aureus, pseudomonas aeruginosa, escherichia coli and ...201526020797
evaluation of the effect of enterococcus faecalis biofilm on the 2% chlorhexidine substantivity: an in vitro study.the aim of this study was to correlate the bacterial viability and the presence of 2% chlorhexidine (chx) solution on dentin by means of confocal laser scanning microscopy and high-performance liquid chromatography for 48 hours, 7 days, and 30 days.201526025346
diversity of enterococcal species and characterization of high-level aminoglycoside resistant enterococci of samples of wastewater and surface water in tunisia.one hundred-fourteen samples of wastewater (n=64) and surface-water (n=50) were inoculated in slanetz-bartley agar plates supplemented or not with gentamicin (sb-gen and sb plates, respectively) for enterococci recovery. enterococci were obtained from 75% of tested samples in sb media (72% in wastewater; 78% in surface-water), and 85 enterococcal isolates (one/positive-sample) were obtained. enterococcus faecium was the most prevalent species (63.5%), followed by enterococcus faecalis (20%), ent ...201526026404
evaluation of cold plasma treatment and safety in disinfecting 3-week root canal enterococcus faecalis biofilm in vitro.although endodontic infection is caused by multi-bacteria species, enterococcus faecalis is usually isolated in chronic apical periodontitis. the aim of this study was to evaluate the effectiveness and mechanical safety of cold plasma therapy in disinfecting 3-week e. faecalis biofilms.201526027875
surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic escherichia coli biofilms against pathogen colonization.prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates production of biomass leading to biofouling of the device. since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. crucial to the success of this strategy ...201526029359
screening currency notes for microbial pathogens and antibiotic resistance genes using a shotgun metagenomic approach.fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome of fomites from various sources. paper currency notes are one of the most commonly exchanged objects and its potential to transmit pathogenic organisms has been well recognized. approaches to identify the microbiome associated with paper currency notes have been largely limited to culture dependent approaches. subsequent studies portrayed the use of 16s ribosomal rna ...201526035208
antibiotic selective pressure and development of bacterial resistance detected in bacteriuria following kidney transplantation.bacteriuria (symptomatic and asymptomatic) is the most common infectious complication after kidney transplantation. this study aimed to determine its prevalence among kidney transplant recipients hospitalized after transplantation, respective risk factors, and frequency of isolates and antibacterial susceptibility.201526036537
antimicrobial resistance profile of enterococcus species isolated from intestinal tracts of hospitalized patients in jimma, ethiopia.multi-drug-resistant enterococci colonizing the intestinal tract of hospitalized patients are the major source of infection as well as nosocomial spread. despite worldwide increasing rate of multidrug resistant enterococci colonization and infection among hospitalized patients, there is scarcity of data from resource limited setting. the present study aimed at determining the antimicrobial resistance profile of enterococcus species from intestinal tracts of hospitalized patients in jimma, ethiop ...201526036911
an assessment of antibacterial activity of three pulp capping materials on enterococcus faecalis by a direct contact test: an in vitro study.the aim of this in vitro study was to evaluate antimicrobial activities of three different pulp capping materials; biodentine, mineral trioxide aggregate (mta) angelus, and dycal against enterococcus faecalis and their durability with time.201726038657
pheromone killing of multidrug-resistant enterococcus faecalis v583 by native commensal strains.multidrug-resistant enterococcus faecalis possess numerous mobile elements that encode virulence and antibiotic resistance traits as well as new metabolic pathways, often constituting over one-quarter of the genome. it was of interest to determine how this large accretion of mobile elements affects competitive growth in the gastrointestinal (gi) tract consortium. we unexpectedly observed that the prototype clinical isolate strain v583 was actively killed by gi tract flora, whereas commensal ente ...201526039987
effects of fixed and removable space maintainers on plaque accumulation, periodontal health, candidal and enterococcus faecalis carriage.to evaluate the effects of space maintainers on plaque accumulation, periodontal health and oral microflora.201526044443
blocking the 'midas' touch of enterococcus faecalis. 201526046076
the in vitro antimicrobial activity of cymbopogon essential oil (lemon grass) and its interaction with silver ions.it is well known that cymbopogon (lemon grass) essential oil exhibits antimicrobial activity while the efficacy of silver ions as a disinfectant is equally well reported.201526055131
expression of interleukin-1β and matrix metalloproteinase-8 in cytolytic and noncytolytic enterococcus faecalis-induced persistent apical periodontitis: a comparative study in the rat.cytolytic enterococcus faecalis possesses a highly toxic and proinflammatory capacity. cytokines and proteases play important roles in the host inflammatory response. the aim of this study was to compare the local expression of interleukin (il)-1β and matrix metalloproteinase-8 (mmp-8) between persistent apical periodontitis (ap) infected by cytolytic and noncytolytic e. faecalis.201526055433
telavancin activity tested against gram-positive clinical isolates from european, russian and israeli hospitals (2011-2013) using a revised broth microdilution testing method: redefining the baseline activity of telavancin.to reassess the activity of telavancin when tested against gram-positive clinical pathogens recovered from hospitalized patients in european and adjacent regions using a revised broth microdilution method.201626058844
purification and characterization of a cysteine-rich 14-kda antibacterial peptide from the granular hemocytes of mangrove crab episesarma tetragonum and its antibiofilm activity.antimicrobial peptide (amp) crustin is a type of immune molecule present in the immune system of crustaceans and response against microbial invasion. in the present study, we have identified and characterized the cationic, amphipathic structure consisting of amp crustin from a mangrove crab episesarma tetragonum using cm sepharose-based cation exchange column chromatography. e. tetragonum crustin showed a single band of 14 kda on sds-page and the homogeneity showed retention time of 8.4 min in r ...201526059067
rampant parasexuality evolves in a hospital pathogen during antibiotic selection.horizontal gene transfer threatens the therapeutic success of antibiotics by facilitating the rapid dissemination of resistance alleles among bacterial species. the conjugative mobile element tn916 provides an excellent context for examining the role of adaptive parasexuality as it carries the tetracycline-resistance allele tetm and has been identified in a wide range of pathogens. we have used a combination of experimental evolution and allelic frequency measurements to gain insights into the a ...201526060280
surface-associated lipoproteins link enterococcus faecalis virulence to colitogenic activity in il-10-deficient mice independent of their expression levels.the commensal enterococcus faecalis is among the most common causes of nosocomial infections. recent findings regarding increased abundance of enterococci in the intestinal microbiota of patients with inflammatory bowel diseases and induction of colitis in il-10-deficient (il-10-/-) mice put a new perspective on the contribution of e. faecalis to chronic intestinal inflammation. based on the expression of virulence-related genes in the inflammatory milieu of il-10-/- mice using rna-sequencing an ...201526067254
rna-based assay demonstrated enterococcus faecalis metabolic activity after chemomechanical procedures.because ribosomal rna (rrna) indicates metabolic cell activity, this study aimed to evaluate the sensitivity of rrna-based quantitative polymerase chain reaction (rt-qpcr) for the identification of active enterococcus faecalis in root canals samples compared with a method based on ribosomal dna (rdna) (rrna genes).201526071099
antimicrobial impacts of essential oils on food borne-pathogens.the antimicrobial activity of twelve essential oil (pine oil, eucalyptus, thyme, sage tea, lavender, orange, laurel, lemon, myrtle, lemon, rosemary and juniper) was tested by a disc diffusion method against food borne pathogens (escherichia coli, salmonella paratyphi a, klebsiella pneumoniae, yersinia enterocolitica, pseudomonas aeruginosa, aeromonas hydrophila, campylobacter jejuni, enterococcus faecalis, staphylococcus aureus). the major components in essential oils were monoterpenes hydrocarb ...201526072990
genetic relationships among enterococcus faecalis isolates from different sources as revealed by multilocus sequence typing.enterococcus faecalis is part of the natural gut flora of humans and other mammals; some isolates are also used in food production. so, it is important to evaluate the genetic diversity and phylogenetic relationships among e. faecalis isolates from different sources. multilocus sequence typing protocol was used to compare 39 e. faecalis isolates from chinese traditional food products (including dairy products, acidic gruel) and 4 published e. faecalis isolates from other sources including human- ...201526074239
probiotic bifico cocktail ameliorates helicobacter pylori induced gastritis.to determine the protective effect of triple viable probiotics on gastritis induced by helicobacter pylori (h. pylori) and elucidate the possible mechanisms of protection.201526074694
effect of in-feed administration and withdrawal of tylosin phosphate on antibiotic resistance in enterococci isolated from feedlot steers.tylosin phosphate is a macrolide commonly administered to cattle in north america for the control of liver abscesses. this study investigated the effect of in-feed administration of tylosin phosphate to cattle at subtherapeutic levels and its subsequent withdrawal on macrolide resistance using enterococci as an indicator bacterium. fecal samples were collected from steers that received no antibiotics and steers administered tylosin phosphate (11 ppm) in-feed for 197 days and withdrawn 28 days be ...201526074889
evaluation of the enterococcus faecalis biofilm-associated virulence factors ahrc and eep in rat foreign body osteomyelitis and in vitro biofilm-associated antimicrobial resistance.enterococcus faecalis can cause healthcare-associated biofilm infections, including those of orthopedic devices. treatment of enterococcal prosthetic joint infection is difficult, in part, due to biofilm-associated antimicrobial resistance. we previously showed that the e. faecalis og1rf genes ahrc and eep are in vitro biofilm determinants and virulence factors in animal models of endocarditis and catheter-associated urinary tract infection. in this study, we evaluated the role of these genes in ...201526076451
non-linear significant relationship between use of glycopeptides and isolation of vancomycin-resistant enterococcus species in a university hospital setting.emergence of colonization and infection with vancomycin-resistant enterococci (vre) has become a worldwide challenge. to investigate whether the increasing incidence of vre isolation can be correlated with use of glycopeptides in the hospital setting, we conducted a hospital-wide two-year study in the university hospital of vienna.201526078865
adherence and intracellular survival within human macrophages of enterococcus faecalis isolates from coastal marine sediment.enterococcus faecalis is part of the human intestinal microbiota and an important nosocomial pathogen. it can be found in the marine environment, where it is also employed as a fecal indicator. to assess the pathogenic potential of marine e. faecalis, four strains isolated from marine sediment were analyzed for their ability to survive in human macrophages. escherichia coli dh5α was used as a negative control. the number of adherent and intracellular bacteria was determined 2.5 h after the infec ...201526079735
phenotypic and genotypic characterization of lactic acid bacteria isolated from raw goat milk and effect of farming practices on the dominant species of lactic acid bacteria.lactic acid bacteria, in particular lactococcus lactis, play a decisive role in the cheese making process and more particularly in lactic cheeses which are primarily produced on goat dairy farms. the objective of this study was therefore to identify the main lactic acid bacteria found in raw goats' milk from three different regions in france and evaluate if certain farming practices have an effect on the distribution of species of lactic acid bacteria in the various milk samples. identification ...201526082325
low-dose gentamicin for uncomplicated enterococcus faecalis bacteremia may be nephrotoxic in children.uncertainty exists regarding the role of synergistic gentamicin for uncomplicated enterococcus faecalis bacteremia in children.201526082503
rna of enterococcus faecalis strain ec-12 is a major component inducing interleukin-12 production from human monocytic cells.interleukin-12 (il-12) is an important cytokine for the immunomodulatory effects of lactic acid bacteria (lab). using murine immune cells, we previously reported that the rna of enterococcus faecalis ec-12, a lab strain exerting probiotic-like beneficial effects, is the major il-12-inducing immunogenic component. however, it was recently revealed that bacterial rna can be a ligand for toll-like receptor (tlr) 13, which is only expressed in mice. because tlr13 is not expressed in humans, the immu ...201526083838
preterm infant gut colonization in the neonatal icu and complete restoration 2 years later.preterm infants in a neonatal intensive care unit (nicu) are exposed to multidrug-resistant bacteria previously adapted to the hospital environment. the aim of the present study was to characterize the bacterial antibiotic-resistant high-risk lineages colonizing preterm infants during their nicu stay and their persistence in faeces after 2 years. a total of 26 preterm neonates were recruited between october 2009 and june 2010 and provided 144 faecal samples. milk samples (86 mother's milk, 35 hu ...201526086569
sol-gel-derived bioactive glasses demonstrate antimicrobial effects on common oral bacteria.to determine the antibacterial effect of nano-structured, sol-gel processed bioactive glasses that may be used for implants, coatings, and as adjuncts to dental restorative materials.201526087578
combined antibacterial effect of sodium hypochlorite and root canal sealers against enterococcus faecalis biofilms in dentin canals.the present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (naocl) and root canal sealers on enterococcus faecalis biofilms using a dentin infection model.201526092772
characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village.in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ...201526095384
selected compounds structurally related to acyclic sesquiterpenoids and their antibacterial and cytotoxic activity.by implementing a common and industrially used method, 30 compounds which are structurally related to geranyl acetone, nerolidol, farnesal, farnesol and farnesyl acetate were obtained. their antimicrobial activity against staphylococcus aureus, enterococcus faecalis, enterococcus faecium, escherichia coli, klebsiella pneumoniae and acinetobacter baumannii bacteria was investigated. pharmacophore models were proposed based on the obtained results and 3d qsar modelling. cytotoxic effects against m ...201526096434
microbiologic evaluation of matricaria and chlorhexidine against e. faecalis and c. albicans.to evaluate the antimicrobial activity of different concentrations of matricaria chamomilla and chlorhexidine gel against candida albicans and enterococcus faecalis.201726097333
antibacterial activity of tribulus terrestris and its synergistic effect with capsella bursa-pastoris and glycyrrhiza glabra against oral pathogens: an in-vitro study.in this study, antimicrobial activities of an ethanol extract of tribulus terrestris aloneand in combination with capsella bursa-pastoris and glycyrrhiza glabra were examined in vitro against six pathogens namely streptococcus mutans, streptococcus sanguis, actinomyces viscosus, enterococcus faecalis staphylococcus aureus, and escherichia coli.201726101754
antibacterial mode of action of the hydroethanolic extract of leonotis nepetifolia (l.) r. br. involves bacterial membrane perturbations.leonotis nepetifolia (l) r. br., lamiaceae, a pantropical shrub, popularly known in brazil as "cordão-de-frade", "rubim", is reportedly used in brazilian ethnomedicine as well as in different countries in the treatments of ailments such as infections, inflammations, wounds, stomach disorders, among others.201526102550
a preliminary investigation on the antimicrobial activity of listerine®, its components, and of mixtures thereof.listerine® is one of the most popular mouthwashes worldwide and claims to combat harmful bacteria. in the past century, its recipe was changed from an essential oil mouthwash to a five-component mixture (thymol, menthol, eucalyptol, and methyl salicylate dissolved in 27% ethanol). the aim of this study was to get preliminary information about the antimicrobial activities of individual listerine® components and their mixtures. we tested the bacterial strains streptococcus mutans, enterococcus fae ...201526104602
[diagnosis in patients with a painful arthroplasty].the differential diagnosis between asepting loosening or prosthetic joint infection is not always easy. tc-99m-labelled leucocyte scans, frozen section and histology can help recognise doubtful cases. we report the experience of the unit for infectious diseases and septic orthopaedics of the asl-2 liguria, italy, with a tc-99m-labelled leucocyte scan and intraoperative frozen section to choose the best therapeutic approach: one-stage or two-stage exchange or arthrodesis-arthroplastica. all cases ...201526110294
the natural surfactant glycerol monolaurate significantly reduces development of staphylococcus aureus and enterococcus faecalis biofilms.bacterial biofilms are involved in a large proportion of clinical infections, including device-related infections. unfortunately, biofilm-associated bacteria are typically less susceptible to antibiotics, and infected devices must often be removed. on the basis of a recent observation that lipid-rich biofilm matrix material is present in early biofilm formation and may protect a population of bacteria from interacting with ordinarily diffusible small molecules, we hypothesized that surfactants m ...201526110557
nanostructured composite material graphite/tio2 and its antibacterial activity under visible light irradiation.the paper addresses laboratory preparation, characterization and in vitro evaluation of antibacterial activity of graphite/tio2 nanocomposites. composites graphite/tio2 with various ratio of tio2 nanoparticles (30wt.%, and 50wt.%) to graphite were prepared using a thermal hydrolysis of titanylsulfate in the presence of graphite particles, and subsequently dried at 80°c. x-ray powder diffraction, transmission electron microscopy and raman microspectroscopy served as phase-analytical methods disti ...201526114221
combination of microbiological culture and multiplex pcr increases the range of vaginal microorganisms identified in cervical cancer patients at high risk for bacterial vaginosis and vaginitis.bacterial vaginosis (bv) and vaginitis in cervical cancer patients might becaused by mixed aerobic, anaerobic, and atypical bacteria. since genital tract infections can be complicated, early and accurate identification of causal pathogens is vital.201526117968
antimicrobial resistance and in vitro biofilm-forming ability of enterococci spp. isolated from urinary tract infection in a tertiary care hospital in dhaka.the biofilm mode of life conveys a survival advantage to the microorganism associated with it. biofilm on an indwelling urinary catheter consists of adherent microorganisms, their extra cellular products, and host components deposited on the catheter and thus biofilm on urinary catheters results in persistent infections that are resistant to antimicrobial therapy. this study was done during the period of january 2010 to december 2010. fifty nine enterococci isolated from 1203 urine samples were ...201426118165
enterococcal endocarditis revisited.the enterococcus species is the third main cause of infective endocarditis (ie) worldwide, and it is gaining relevance, especially among healthcare-associated cases. patients with enterococcal ie are older and have more comorbidities than other types of ie. classical treatment options are limited due to the emergence of high-level aminoglycosides resistance (hlar), vancomycin resistance and multidrug resistance in some cases. besides, few new antimicrobial alternatives have shown real efficacy, ...201526118390
a novel rat model of catheter-associated urinary tract infection.the authors aimed to establish a rat model of catheter-associated utis using a complete urethral catheter. bacterial growth in biofilms on urethral catheters was analyzed using standard culture methods to validate this model.201526122120
codon-optimized fluorescent mtfp and mcherry for microscopic visualization and genetic counterselection of streptococci and enterococci.despite the powerful potential of fluorescent proteins for labeling bacteria, their use has been limited in multi-species oral biofilm models. fermentative metabolism by streptococcal species that initiate biofilm colonization results in an acidic, reduced microenvironment that may limit the activities of some fluorescent proteins which are influenced by ph and oxygen availability. the need to reliably distinguish morphologically similar strains within biofilms was the impetus for this work. tea ...201526122309
the ribosomal s10 protein is a general target for decreased tigecycline susceptibility.tigecycline is a translational inhibitor with efficacy against a wide range of pathogens. using experimental evolution, we adapted acinetobacter baumannii, enterococcus faecium, escherichia coli, and staphylococcus aureus to growth in elevated tigecycline concentrations. at the end of adaptation, 35 out of 47 replicate populations had clones with a mutation in rpsj, the gene that encodes the ribosomal s10 protein. to validate the role of mutations in rpsj in conferring tigecycline resistance, we ...201526124155
from (p)ppgpp to (pp)pgpp: characterization of regulatory effects of pgpp synthesized by the small alarmone synthetase of enterococcus faecalis.the bacterial stringent response (sr) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. this response is mediated by the intracellular accumulation of the alarmones pppgpp and ppgpp, collectively called (p)ppgpp. in enterococcus faecalis, (p)ppgpp metabolism is carried out by the bifunctional synthetase/hydrolase e. faecalis rel (relef) and the small alarmone synthetase (sas) relqef. although rel is the main enzyme responsible for sr ...201526124242
evaluation of an indigenously prepared herbal extract (endopam) as an antimicrobial endodontic irrigant: an ex vivo study.root canal irrigation plays a pivotal role in endodontics. constant increase in antibiotic resistance and side effects caused by synthetic irrigants has shifted the research toward developing herbal alternatives. the current study aims to assess the ex vivo effectiveness of an indigenously prepared herbal extract "endopam" and compare it with the conventional endodontic irrigants for disinfection of root canals infected with enterococcus faecalis.201526124607
evaluation of antimicrobial and antifungal efficacy of inter appointment intracanal medicaments against enterococcus and candida albicans: an in vitro study.microorganisms and their by-products in pulpal and periapical diseases are to be considered as the primary etiological agents of the pulpal necrosis and apical periodontitis. enterococcus faecalis, which is the most common organism isolated from failed root canals, is a gram-positive facultative anaerobe. yeasts can be detected in 7-18% of infected root canals.201526124609
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