Publications

crystal structure of porphyromonas gingivalis dipeptidyl peptidase 4 and structure-activity relationships based on inhibitor profiling.	the gram-negative anaerobe porphyromonas gingivalis is associated with chronic periodontitis. clinical isolates of p. gingivalis strains with high dipeptidyl peptidase 4 (dpp4) expression also had a high capacity for biofilm formation and were more infective. the x-ray crystal structure of p. gingivalis dpp4 was solved at 2.2 å resolution. despite a sequence identity of 32%, the overall structure of the dimer was conserved between p. gingivalis dpp4 and mammalian orthologues. the structures of t ...	2017	28826083
periopathogens differ in terms of the susceptibility to toluidine blue o-mediated photodynamic inactivation.	the main goal of periodontal therapy is to eliminate the infection spreading in periodontium. antimicrobial photodynamic therapy may be applied in order to eradicate pathogens remaining in periodontal tissues after conventional mechanical debridement, thus, improve the treatment results. the aim of this in vitro study was to evaluate the susceptibility of selected key periopathogens to toluidine blue o-mediated photodynamic inactivation and the influence of photosensitizer's concentration and li ...	2017	28826733
different engagement of tlr2 and tlr4 in porphyromonas gingivalis vs. ligature-induced periodontal bone loss.	this study was conducted to investigate the roles of different toll-like receptor (tlr) signaling in porphyromonas gingivalis (p. gingivalis)-induced and ligature-induced experimental periodontal bone resorption in mice. wild-type (wt), tlr2 knockout (ko), tlr4ko, and tlr2&4 ko mice with c57/bl6 background were divided into three groups: control, p. gingivalis infection, and ligation. live p. gingivalis or silk ligatures were placed in the sulcus around maxillary second molars over a 2-week peri ...	2017	28832712
the case for periodontitis in the pathogenesis of rheumatoid arthritis.	rheumatoid arthritis (ra), an autoimmune disease that affects ∼1% of the human population, is driven by autoantibodies that target modified self-epitopes, whereas ∼11% of the global adult population are affected by severe chronic periodontitis, a disease in which the commensal microflora on the tooth surface is replaced by a dysbiotic consortium of bacteria that promote the chronic inflammatory destruction of periodontal tissue. despite differences in aetiology, ra and periodontitis are similar ...	2017	28835673
investigation of potential targets of porphyromonas crisprs among the genomes of porphyromonas species.	the oral bacterial species porphyromonas gingivalis, a periodontal pathogen, has plastic genomes that may be driven by homologous recombination with exogenous deoxyribonucleic acid (dna) that is incorporated by natural transformation and conjugation. however, bacteriophages and plasmids, both of which are main resources of exogenous dna, do not exist in the known p. gingivalis genomes. this could be associated with an adaptive immunity system conferred by clustered regularly interspaced short pa ...	2017	28837670
relatively low invasive capacity of porphyromonas gingivalis strains into human gingival fibroblasts in vitro.	bacterial invasion into host cells is a common strategy to escape the host immune system. gingival fibroblasts (gfs) are the most predominant non-phagocytic cell type in gingival connective tissue. therefore, invasion into gfs was thought to be the first strategy for the survival of porphyromonas gingivalis. the present study compared the invasive ability of p. gingivalis into gfs with those of other red-complex and relatively less pathogenic bacterial strains, especially fusobacterium nucleatum ...	2017	28841474
microbiological response to periodontal therapy and multivariable prediction of clinical outcome.	this study assessed the microbiological effects of a 2-phase antimicrobial periodontal therapy and tested microbiological, clinical, and biological markers as prognostic indicators for clinical success.	2017	28844191
corrigendum: γ-ionylidene-type sesquiterpenoids possessing antimicrobial activity against porphyromonas gingivalis from phellinus linteus and their absolute structure determination.	this corrects the article doi: 10.1038/ja.2017.35.	2017	28845046
the oral microbiome and adverse pregnancy outcomes.	significant evidence supports an association between periodontal pathogenic bacteria and preterm birth and preeclampsia. the virulence properties assigned to specific oral pathogenic bacteria, for example, fusobacterium nucleatum, porphyromonas gingivalis, filifactor alocis, campylobacter rectus, and others, render them as potential collaborators in adverse outcomes of pregnancy. several pathways have been suggested for this association: 1) hematogenous spread (bacteremia) of periodontal pathoge ...	2017	28848365
quantification by qpcr of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level.	currently, there is little evidence available on the development of predictive models for the diagnosis or prognosis of chronic periodontitis based on the qpcr quantification of subgingival pathobionts. our objectives were to: (1) analyze and internally validate pathobiont-based models that could be used to distinguish different periodontal conditions at site-specific level within the same patient with chronic periodontitis; (2) develop nomograms derived from predictive models. subgingival plaqu ...	2017	28848499
porphyromonas gingivalis stimulates tlr2-pi3k signaling to escape immune clearance and induce bone resorption independently of myd88.	porphyromonas gingivalis is a gram-negative anaerobic periodontal pathogen that persists in dysbiotic mixed-species biofilms alongside a dense inflammatory infiltrate of neutrophils and other leukocytes in the subgingival areas of the periodontium. toll-like receptor 2 (tlr2) mediates the inflammatory response to p. gingivalis and tlr2-deficient mice resist alveolar bone resorption following oral challenge with this organism. although, myd88 is an adaptor protein considered necessary for tlr2-in ...	2017	28848717
preventive effects of the novel antimicrobial peptide nal-p-113 in a rat periodontitis model by limiting the growth of porphyromonas gingivalis and modulating il-1β and tnf-α production.	p-113 (akrhhgykrkfh-nh2) is a 12-amino-acid histidine-rich peptide derived from histatin 5 that is highly degradable in high salt concentrations and biological fluids such as serum, plasma and saliva. nal-p-113, a novel antimicrobial peptide whose histidine residues are replaced by the bulky amino acids β-naphthylalanine, causes the antimicrobial peptide to retain its bactericidal activity even in physiological environments. this study evaluated the effect of the novel antimicrobial peptide nal- ...	2017	28851350
risk of superinfection in peri-implantitis after systemic broad spectrum antibiotics.	peri-implant disease has developed over the last few years as a complication that is often difficult to resolve. the disease process is mainly attributed to bacterial infection. proposed combined therapies use broad-spectrum antibiotics to halt its progression. a major associated risk is the undetected development of superinfections that are difficult to eradicate. a group of healthy individuals with advanced peri-implantitis (pi) were referred for evaluation due to severe, rapidly progressive b ...	2017	28854287
therapeutic applications of resveratrol and its derivatives on periodontitis.	periodontitis is an inflammatory disease of the supporting tissues of the teeth induced by periodontopathic bacteria that results in the progressive destruction of periodontal tissues. treatment of periodontitis is painful and time-consuming. recently, herbal medicines have been considered for use in treating inflammation-related diseases, including periodontitis. resveratrol and its derivative 2,3,5,4'-tetrahydroxystilbene-2-o-β-glucoside (thsg), a polyphenol extracted from polygonum multifloru ...	2017	28856691