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inhibition of hmg-coa reductase by mfs, a purified extract from the fermentation of marine fungus fusarium solani fg319, and optimization of mfs production using response surface methodology.the present study was designed to isolate and characterize a purified extract from fusarium solani fg319, termed mfs (metabolite of fusarium solani fg319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase. response surface methodology (rsm) was employed to achieve an improved yield from the fermentation medium. the inhibiting effect of the isolate, mfs, on hmg-coa reductase was greater than that of the positive control, lovastatin. ...201525986283
molecular mechanisms underlying the close association between soil burkholderia and fungi.bacterial species belonging to the genus burkholderia have been repeatedly reported to be associated with fungi but the extent and specificity of these associations in soils remain undetermined. to assess whether associations between burkholderia and fungi are widespread in soils, we performed a co-occurrence analysis in an intercontinental soil sample collection. this revealed that burkholderia significantly co-occurred with a wide range of fungi. to analyse the molecular basis of the interacti ...201525989372
emergence of fusarioses in a university hospital in turkey during a 20-year period.fusarium species have started appearing increasingly as the main cause of infections, particularly in immunocompromised patients. in this study, we aimed to present the first epidemiological data from turkey, analyze fusariosis cases that have been monitored in a university hospital during the past 20 years, identify the responsible fusarium species, and determine antifungal susceptibilities. a total of 47 cases of fusariosis was included in the study. fusarium isolates were identified by multil ...201525994844
keratitis caused by moulds in santa lucía ophthalmology hospital in buenos aires, argentina.mycotic keratitis by moulds (mkm) is an important cause for corneal blindness and usually carries an unfavorable prognosis.201625999088
antimicrobial, antitumor and brine shrimp lethality assay of ranunculus arvensis l. extracts.to investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of methanol extract (me), water extract (we), acetone extract (ae), chloroform extract (ce), methanol-water extract (mwe), methanol-acetone extract (mae), methanol-chloroform extract (mce) of ranunculus arvensis (l.). antitumor activity was evaluated with agrobacterium tumefaciens (at10) induced potato disc assay. cytotoxicity was evaluated with brine shrimp lethality assay. antibacterial a ...201526004705
sertraline enhances the activity of antimicrobial agents against pathogens of clinical relevance.serotonin reuptake inhibitors were recently reported to possess antimicrobial potentials, potentiate activity of several antibiotics, reverse multidrug resistant phenotypes of bacteria and make them susceptible to previously resistant drugs. we investigated antimicrobial potentials of sertraline (sr) against atcc strains, clinical isolates of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa alone and in-combination with seven antibiotics. antifungal activity was investigated ag ...201526029671
broad-spectrum antimicrobial/antifouling soft material coatings using poly(ethylenimine) as a tailorable scaffold.microbial colonization and biofilm formation is the leading cause of contact lens-related keratitis. treatment of the condition remains a challenge because of the need for prolonged therapeutic course and high doses of antimicrobial agents especially for biofilm eradication. the development of strategies to prepare nonfouling contact lens surfaces is a more practical way to ensure users' safety and relieve the excessive public healthcare burden. in this study, we report a series of polymers that ...201526039032
production of 4-ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of ipomoea batatas lam.: a comparative study.a comparative study was done on the production of 4-ipomeanol from root tubers of ipomoea batatas and its rhizogenic callus. best callusing response was obtained on ms medium supplemented with 11 μm naa (α-naphthaleneacetic acid) and 1 μm kin (kinetin). effect of various elicitors (fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. methanol extract of the samples were purified by column chromatography and detected using tlc. identification of 4-ipomeanol was conf ...201526040027
three new cutinases from the yeast arxula adeninivorans that are suitable for biotechnological applications.the genes acut1, acut2, and acut3, encoding cutinases, were selected from the genomic dna of arxula adeninivorans ls3. the alignment of the amino acid sequences of these cutinases with those of other cutinases or cutinase-like enzymes from different fungi showed that they all had a catalytic s-d-h triad with a conserved g-y-s-q-g domain. all three genes were overexpressed in a. adeninivorans using the strong constitutive tef1 promoter. recombinant 6× his (6h)-tagged cutinase 1 protein (p) from a ...201526048925
effects of fusarium solani and f. oxysporum infection on the metabolism of ginsenosides in american ginseng roots.american ginseng (panax quinquefolius l.) is a highly valuable herb widely used for medicinal treatments. its pharmacologically important compounds are the ginsenosides, which are secondary metabolites in american ginseng root. the concentrations of ginsenoside in roots can be changed by fungal infection, but it is unclear what specific root tissues are impacted and whether the change is systemic. in this study, american ginseng roots were inoculated with two fungal pathogens (fusarium solani or ...201526060917
chemical composition, antioxidant and antimicrobial activities of basil (ocimum basilicum) essential oils depends on seasonal variations.chemical composition, antioxidant and antimicrobial activities of the essential oils from aerial parts of basil (ocimum basilicum l.) as affected by four seasonal, namely summer, autumn, winter and spring growing variation were investigated. the hydro-distilled essential oils content ranged from 0.5% to 0.8%, the maximum amounts were observed in winter while minimum in summer. the essential oils consisted of linalool as the most abundant component (56.7-60.6%), followed by epi-α-cadinol (8.6-11. ...200826065762
screen for soil fungi highly resistant to dichloroaniline uncovers mostly fusarium species.arylamines are frequent pollutants in soils. fungi have proven to be efficient in detoxifying these chemicals by acetylating them using arylamine n-acetyl transferase enzymes. here, we selected from natural soils fungi highly resistant to 3,4-dichloroaniline (dca). fusarium species were the most frequently isolated species, especially fusarium solani. the sequenced strain of f. solani contains five nat genes, as did all the dca-resistant isolates. rt-pcr analysis showed that the five genes were ...201526068093
fatal fusarium solani species complex infections in elasmobranchs: the first case report for black spotted stingray (taeniura melanopsila) and a literature review.fusarium species are environmental saprophytic fungi. among the many fusarium species, members of the fusarium solani species complex (fssc) are the most prevalent and virulent in causing human and animal infections. in this study, we describe the first case of fatal fssc infection in a black spotted stingray and three concomitant infections in scalloped hammerhead sharks. in the stingray, cutaneous lesions were characterised by ulcers and haemorrhage of the ventral pectoral fin, or 'ray', espec ...201526095191
diversity of endophytic fungi in glycine max.endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. with the isolation and identification of these fungi, new species are being discovered, and ecological relationships with their hosts have also been studied. in glycine max, limited studies have investigated the isolation and distribution of endophytic fungi throughout leaves and roots. the distribution of these fungi in various plant organs differs in diversity and abundan ...201526111593
anatomy of a nonhost disease resistance response of pea to fusarium solani: pr gene elicitation via dnase, chitosan and chromatin alterations.of the multiplicity of plant pathogens in nature, only a few are virulent on a given plant species. conversely, plants develop a rapid "nonhost" resistance response to the majority of the pathogens. the anatomy of the nonhost resistance of pea endocarp tissue against a pathogen of bean, fusarium solani f.sp. phaseoli (fsph) and the susceptibility of pea to f. solani f sp. pisi (fspi) has been described cytologically, biochemically and molecular-biologically. cytological changes have been followe ...201526124762
bioprocess development for docosahexaenoic acid from novel fungal isolate of fusarium solani.fungal cultures were isolated from soil samples collected from the western ghats regions of kerala. primary screening of isolated strains were done by sudan black staining method and 15 lipid producing cultures were isolated. the fatty acid profiling of the positive strains were analyzed for docosahexaenoic acid (dha) production. selected oleaginous cultures were grown in submerged culture condition to study the biomass yield and poly unsaturated fatty acid, dha production. the optimization of p ...201526155676
antifungal efficiency of a lipopeptide biosurfactant derived from bacillus subtilis spb1 versus the phytopathogenic fungus, fusarium solani.bacillus subtilis spb1 lipopeptides were evaluated as a natural antifungal agent against fusarium solani infestation. in vitro antifungal assay showed a minimal inhibitory concentration of about 3 mg/ml with a fungicidal mode of action. in fact, treatment of f. solani by spb1 lipopeptides generated excessive lyses of the mycelium and caused polynucleation and destruction of the related spores together with a total inhibition of spore production. furthermore, an inhibition of germination potency ...201526178831
a single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens.a novel lysozyme from cauliflower was purified in a single step, for the first time, using sephadex g100 column chromatography. the purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its molecular mass was calculated to be 22.0 kda. the purified lysozyme showed activity between 30 to 60 °c with 40 °c as the optimum temperature for its maximal activity. although the purified lysozyme was functional at ph ranges betwee ...201526208688
biotechnological prospecting of foliar endophytic fungi of guaco (mikania glomerata spreng.) with antibacterial and antagonistic activity against phytopathogens.mikania glomerata (spreng.), popularly known as "guaco", is a plant from the asteraceae family that has many therapeutic properties. the use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. microorganism-host complex interactions are related to the production of compounds that may confer resistance to pathogens or to production of bioactive compounds or growth regulators. in this study, we ...201526214408
endophytic bacteria from piper tuberculatum jacq.: isolation, molecular characterization, and in vitro screening for the control of fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (piper nigrum l.).endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. in this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae piper tuberculatum, which is known for its resistance to infection by fusarium solani f. sp piperis, the causal agent of black pepper (piper nigrum) root rot disease in the amazon region. based on 16s rrna gene sequence ...201526214435
selection and molecular identification of fungal isolates that produce xylanolytic enzymes.xylan is a heteropolysaccharide and its complete hydrolysis involves a complex set of xylanolytic enzymes. fungal xylanases have been widely used in the holocellulose industry to obtain by-products or for its elimination. the aim of this study was to select and identify filamentous fungi from different ecosystems that produce extracellular xylanases showing biotechnological potential. one hundred three fungal isolates were obtained from orchard, horticultural, and forest ecosystems. the ability ...201526214493
evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry bruker biotyper for identification of penicillium marneffei, paecilomyces species, fusarium solani, rhizopus species, and pseudallescheria boydii.we evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms), the maldi bruker biotyper system (microflex lt; bruker daltonik gmbh, bremen, germany), on the identification of 50 isolates of clinically encountered molds, including penicillium marneffei (n = 28), paecilomyces species (n = 12), fusarium solani (n = 6), rhizopus species (n = 3), and pseudallescheria boydii (n = 1). the isolates were identified to species levels by sequen ...201526217315
new constitutive latex osmotin-like proteins lacking antifungal activity.proteins that share similar primary sequences to the protein originally described in salt-stressed tobacco cells have been named osmotins. so far, only two osmotin-like proteins were purified and characterized of latex fluids. osmotin from carica papaya latex is an inducible protein lacking antifungal activity, whereas the calotropis procera latex osmotin is a constitutive antifungal protein. to get additional insights into this subject, we investigated osmotins in latex fluids of five species. ...201526231325
a comparative analysis of distribution and conservation of microsatellites in the transcripts of sequenced fusarium species and development of genic-ssr markers for polymorphism analysis.we used an in silico approach to survey and compare microsatellites in transcript sequences of four sequenced members of genus fusarium. g + c content of transcripts was found to be positively correlated with the frequency of ssrs. our analysis revealed that, in all the four transcript sequences studied, the occurrence, relative abundance and density of microsatellites varied and was not influenced by transcript sizes. no correlation between relative abundance and transcript sizes was observed. ...201526269381
germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants.controlled transgene expression via a promoter is particularly triggered in response to pathogen infiltration. this is significant for eliciting disease-resistant features in crops through genetic engineering. the germins and germin-like proteins (glps) are known to be associated with plant and developmental stages. the 1107-bp oryza sativa root glp2 (osrglp2) gene promoter fused to a β-glucuronidase (gus) reporter gene was transformed into potato plants through an agrobacterium-mediated transfo ...201626277722
structural and functional studies of a fusarium oxysporum cutinase with polyethylene terephthalate modification potential.cutinases are serine hydrolases that degrade cutin, a polyester of fatty acids that is the main component of plant cuticle. these biocatalysts have recently attracted increased biotechnological interest due to their potential to modify and degrade polyethylene terephthalate (pet), as well as other synthetic polymers.201526291558
fungal peritonitis due to fusarium solani species complex sequential isolates identified with dna sequencing in a kidney transplant recipient in brazil.fungal peritonitis is a rare serious complication most commonly observed in immunocompromised patients under peritoneal dialysis. nevertheless, this clinical condition is more difficult to treat than bacterial peritonitis. bacterial peritonitis followed by the use of antibiotics is the main risk factor for developing fungal peritonitis. candida spp. are more frequently isolated, and the isolation of filamentous fungi is only occasional. here we describe a case of fusarium solani species complex ...201526296944
biocontrol of tomato plant diseases caused by fusarium solani using a new isolated aspergillus tubingensis ctm 507 glucose oxidase.the present study focuses on the potential of glucose oxidase (god) as a promising biocontrol agent for fungal plant pathogens. in fact, a new god producing fungus was isolated and identified as an aspergillus tubingensis. god (125 au) has been found to inhibit fusarium solani growth and spore production. indeed, god caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. in vivo assays, god acted as a curative treatment ...201526299190
rhizopus arrhizus and fusarium solani concomitant infection in an immunocompromised host.neutropenic patients are at risk of the development of hyalohyphomycosis and mucormycosis. correct identification is essential for the initiation of the specific treatment, but concomitant mold infections are rarely reported. we report one unprecedented case of concomitant mucormycosis and fusariosis in a neutropenic patient with acute myeloid leukemia. the patient developed rhino-orbital infection by rhizopus arrhizus and disseminated infection by fusarium solani. the first culture from a sinus ...201626346377
phylogenetic diversity of human pathogenic fusarium and emergence of uncommon virulent species.fusarium species cause a broad spectrum of infections. however, little is known about the etiological agents to the species level. we identified fusarium species isolated from clinical specimens including those of high risk patients to better understand the species involved in the pathogenesis.201526348828
antagonistic potential of native trichoderma viride strain against potent tea fungal pathogens in north east india.indigenous strains of trichoderma species isolated from rhizosphere soils of tea gardens of assam, north eastern state of india were assessed for in vitro antagonism against two important tea fungal pathogens namely pestalotia theae and fusarium solani. a potent antagonist against both tea pathogenic fungi, designated as sdrlin1, was selected and identified as trichoderma viride. the strain also showed substantial antifungal activity against five standard phytopathogenic fungi. culture filtrate ...201526361476
role of antagonistic microorganisms and organic amendment in stimulating the defense system of okra against root rotting fungi.without application of chemical pesticides control of soilborne diseases is a great challenge. stimulation of natural plant's defense is considered as one of the most promising alternative strategy for crop protection. organic amendment of soil besides direct suppressing the pathogen, has been reported to have an influence on phytochemicals in plants. in the present study, pseudomonas aeruginosa, a plant growth promoting rhizobacterium and paecilomyces lilacinus, an egg parasite of root knot and ...201526373176
cd34+ highly enriched allogeneic stem cell transplantation in a patient with mixed phenotype acute leukemia and fusarium solani sepsis. 201626382275
ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease.the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ...201526417353
cluster of fusarium solani isolations in a bronchoscopy unit. 201626417853
response surface optimization for decaffeination and theophylline production by fusarium solani.coffee processing industries generate caffeine-containing waste that needs to be treated and decaffeinated before being disposed. five fungal isolates obtained on caffeine-containing mineral media were tested for their ability to utilize caffeine at high concentrations. an isolate identified as fusarium solani could utilize caffeine as a sole source of carbon and nitrogen up to 5 g/l and could degrade it to an extent of 30-53 % in 120 h. sucrose that was added as an auxiliary substrate (5 g/l) e ...201626419659
molecular identification of fusarium species isolated from transgenic insect-resistant cotton plants in mexicali valley, baja california.cotton production in the mexicali valley is adversely affected by wilt and root rot disease associated with fusarium species. in the present study, we sought to isolate and identify the fusarium species in the rhizosphere of transgenic insect-resistant cotton plants grown in the mexicali valley. our analyses isolated four native fungi from the rhizosphere of cotton plants, namely, t-ica01, t-ica03, t-ica04, and t-ica08. these fungal isolates were categorized as belonging to fusarium solani using ...201526436498
etiological analysis of fungal keratitis and rapid identification of predominant fungal pathogens.fungal keratitis is a worldwide-distributed refractory and potentially blinding ocular infection caused by various fungi. it is necessary to investigate the etiological and epidemiological characteristics of this disease and establish a rapid and specific pathogenic identification method. here, we isolated and identified fungal pathogens of 275 patients with presumed fungal keratitis from jilin province, china, and conducted statistical analyses of epidemiological information. the positive rate ...201626446032
crystal structure of an antifungal osmotin-like protein from calotropis procera and its effects on fusarium solani spores, as revealed by atomic force microscopy: insights into the mechanism of action.cposm is an antifungal osmotin/thaumatin-like protein purified from the latex of calotropis procera. the protein is relatively thermostable and retains its antifungal activity over a wide ph range; therefore, it may be useful in the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogenic fungi. to gain further insight into the mechanism of action of cposm, its three-dimensional structure was determined, and the effects of the protein on fusarium solani ...201526456062
phylogenetic analysis of fusarium solani associated with the asian longhorned beetle, anoplophora glabripennis.culture-independent analysis of the gut of a wood-boring insect, anoplophora glabripennis (coleoptera: cerambycidae), revealed a consistent association between members of the fungal fusarium solani species complex and the larval stage of both colony-derived and wild a. glabripennis populations. using the translation elongation factor 1-alpha region for culture-independent phylogenetic and operational taxonomic unit (otu)-based analyses, only two otus were detected, suggesting that genetic varian ...201226467954
synthesis of heterocycle-attached methylidenebenzenesulfonohydrazones as antifungal agents.a series of heterocycle-attached methylidenebenzenesulfonohydrazone derivatives were synthesized and evaluated for their antifungal activities against seven phytopathogenic fungi such as fusarium graminearum, alternaria solani, valsa mali, phytophthora capsici, fusarium solani, botrytis cinerea, and glomerella cingulata. compounds 7b, 8d, 9a, 9b and 9d exhibited a good and broad-spectrum of antifungal activities against at least five phytopathogenic fungi at the concentration of 100 μg/ml. it de ...201526471091
fungal infections after boston type 1 keratoprosthesis implantation: literature review and in vitro antifungal activity of hypochlorous acid.to review the current literature describing cases of fungal keratitis and endophthalmitis after boston keratoprosthesis (kpro) implantation and to characterize the antifungal activity of 0.01% hypochlorous acid against medically relevant fungi.201526488624
[identification of pathogens causing root rot disease on ligusticum chuanxiong in sichuan].to identify the pathogens causing root rot disease on ligusticum chuanxiong from the main producing areas in sichuan, and to provide gist of management of this disease in the future.201526495640
antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum.bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ...201526496638
the fusarium solani species complex: ubiquitous pathogens of agricultural importance.members of the fusarium solani species complex (fssc) are capable of causing disease in many agriculturally important crops. the genomes of some of these fungi include supernumerary chromosomes that are dispensable and encode host-specific virulence factors. in addition to genomics, this review summarizes the known molecular mechanisms utilized by members of the fssc in establishing disease.201626531837
fatal cases of bloodstream infection by fusarium solani and review of published literature.fusarium species are ubiquitously present in environment and are well known as human pathogens with high mortality rate in immunocompromised patients. we report here two cases where immunocompromised patients developed fatal bloodstream infections by this organism. isolates were further identified by its1 region sequencing which confirmed them as fusarium solani. antifungal susceptibility testing was done following clsi m38-a2 guidelines to amphotericin b, fluconazole, itraconazole, voriconazole ...201626541869
expression of vitamin d receptor and cathelicidin in human corneal epithelium cells during fusarium solani infection.to observe the expression of vitamin d receptor (vdr) in human specimen and immortalized human corneal epithelium cells (hcec) when challenged with fusarium solani. moreover, we decided to discover the pathway of vdr expression. also, we would like to detect the expression of cathelicidin antimicrobial peptide (camp) in the downstream pathway of vdr.201526558193
pseudomonas induces salinity tolerance in cotton (gossypium hirsutum) and resistance to fusarium root rot through the modulation of indole-3-acetic acid.abiotic stresses cause changes in the balance of phytohormones in plants and result in inhibited root growth and an increase in the susceptibility of plants to root rot disease. the aim of this work was to ascertain whether microbial indole-3-acetic acid (iaa) plays a role in the regulation of root growth and microbially mediated control of root rot of cotton caused by fusarium solani. seed germination and seedling growth were improved by both nacl and mg2so4 (100 mm) solutions when treated with ...201526587006
effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi.mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ...201626619828
secretome analysis of the mycoparasitic fungus trichoderma harzianum all 42 cultivated in different media supplemented with fusarium solani cell wall or glucose.trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. the aim of this study was to characterize the proteins secreted by t. harzianum all42 when its spores were inoculated and incubated for 48 h in culture media supplemented with glucose (glu) or with cell walls from fusarium solani (fscw), a phytopathogen that causes severe losses in common bean and soy crops in brazil, as well as other crop diseases around the world. trichoderm ...201626631988
evaluation of antifungal efficacy of 0.1% and 0.25% riboflavin with uva: a comparative in vitro study.antifungal efficacy of photochemical cross-linking (pack-cxl) with 0.1% and 0.25% riboflavin was evaluated with a comparative in vitro study.201626644282
multilocus phylogeny reveals an association of agriculturally important fusarium solani species complex (fssc) 11, and clinically important fssc 5 and fssc 3 + 4 with soybean roots in the north central united states.the fusarium solani species complex (fssc) includes important root pathogens of soybean in the united states, but the evolutionary lineages associated with soybean root rot are unknown. a multilocus phylogeny based on 93 isolates from soybean and pea roots from north dakota and minnesota revealed that root rot was associated with three known phylogenetic species, fssc 3 + 4 (=fusarium falciforme) (3 % of isolates), fssc 5 (60 %), fssc 11 (34 %), and one unknown species, fssc x (2 %). of these sp ...201626671414
pathogenic ability and saline stress tolerance of two fusarium isolates from odontesthes bonariensis eggs.several fungal species represent a potential risk to embryos of odontesthes bonariensis (cuvier and valenciennes, 1835), a euryhaline freshwater fish that lives in the pampean inland waters and has potential economic relevance.201626674586
heterologous overexpression and biochemical characterization of the (galactophospho)lipase from fusarium solani in pichia pastoris that is expressed in planta.high-level extracellular production of fusarium solani (galactophospho)lipase, named fsl, was achieved using a pichia pastoris x33 expression system. the (galactophospho) lipase encoding gene was cloned into pgapzαa with the saccharomyces cerevisiae α-factor signal sequence by two different ways. the two constructs consist of an additional sequence of a (his)6-tag of the vector fused to the n-terminus of this enzyme (tfsl) while the other expression vector was constructed without any additional ...201626675137
roles of triolein and lipolytic protein in the pathogenesis and survival of mycobacterium tuberculosis: a novel therapeutic approach.discovery of novel secreted enzymes and proteins in mycobacterium tuberculosis (m. tuberculosis) are imperative to understanding the pathogenic system for pathogenesis requires attention. till date, the groups of these secreted enzymes are not meaningfully characterized in terms of m. tuberculosis. in this way, cutinase, a small lipolytic protein, exists in both bacteria and fungi as well which have a potential catalytic activity. during our search, we have found a few genes of m. tuberculosis d ...201626679705
novel bio-essential metal based complexes linked by heterocyclic ligand: synthesis, structural elucidation, biological investigation and docking analysis.new series of bio-essential metal based complexes linked by schiff base ligand (l) and 2,2'-bipyridine (bpy) have been synthesized and characterized by diverse spectral techniques such as elemental analysis, magnetic susceptibility, molar conductivity measurements, ft-ir, uv-vis., (1)h nmr, (13)c nmr, epr and mass. the spectral data suggest that the metal complexes espouse octahedral geometry around the metal ions. interactions of the complexes with ct dna have been explored by electronic absorp ...201626690017
[molecular identification and in vitro susceptibility of fusarium from fungal keratitis in central china].to detect the genotypes and in vitro antifungal susceptibility of fusarium isolated from patients with fungal keratitis in central china.201526693650
fusarium paranaense sp. nov., a member of the fusarium solani species complex causes root rot on soybean in brazil.isolates of fusarium obtained from soybean plants showing symptoms of root rot collected in subtropical southern and tropical central brazil were characterized based on phylogenetic analyses, sexual crossing, morphology, and pathogenicity tests. a novel species within the fusarium solani species complex (fssc) causing soybean root rot is formally described herein as fusarium paranaense. this species can be distinguished from the other soybean root rot pathogens in the fssc, which are commonly as ...201626693684
fusarium solani endocarditis in an acute leukemia patient. 201626706407
structural analysis of glucosylceramides (glccer) from species of the pseudallescheria/scedosporium complex.glucosylceramides (glccer) are the main neutral glycosphingolipids expressed in fungal cells. in this work, glucosylceramides (glccer) were extracted from three strains of scedosporium (pseudallescheria) boydii, one strain of pseudallescheria ellipsoidea and one strain of pseudallescheria angusta and purified by several chromatographic steps. using high-performance thin layer chromatography (hptlc), we found a similarity between glccer obtained from all of the analysed strains. a detailed struct ...201626781373
ag doped hollow tio2 nanoparticles as an effective green fungicide against fusarium solani and venturia inaequalis phytopathogens.chemical-based pesticides are widely used in agriculture to protect crops from insect infestation and diseases. however, the excessive use of highly toxic pesticides causes several human health (neurological, tumor, cancer) and environmental problems. therefore nanoparticle-based green pesticides have become of special importance in recent years. the antifungal activities of pure and ag doped (solid and hollow) tio2 nanoparticles are studied against two potent phytopathogens, fusarium solani (wh ...201626808118
effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte fusarium solani.volumetric productivity of camptothecin from the suspension culture of the endophyte fusarium solani was enhanced up to ∼152 fold (from 0.19 μg l(-1) d(-1) to 28.9 μg l(-1) d(-1)) under optimized fermentation conditions including initial ph (6.0), temperature (32 °c) and agitation speed (80 rpm) with (5% (v/v)) ethanol as medium component. among various elicitors and precursors studied, tryptamine (0.5 mm) as precursor and bovine serum albumin (bsa) (0.075 mm) as an elicitor added on day 6 of th ...201626851893
studying of cellular interaction of hairpin-like peptide ecamp1 from barnyard grass (echinochloa crusgalli l.) seeds with plant pathogenic fungus fusarium solani using microscopy techniques.an interaction of recombinant hairpin-like cationic peptide ecamp1 with conidia of plant pathogenic fungus fusarium solani at the cellular level was studied by a combination of microscopic methods. ecamp1 is from barnyard grass (echinochloa crusgalli l.), and obtained by heterologous expression in escherichia coli system. as a result, a direct relationship between hyphal growth inhibition and increasing active peptide concentration, time of incubation and fungal physiological condition has been ...201626855384
molecular phylogenetics and anti-pythium activity of endophytes from rhizomes of wild ginger congener, zingiber zerumbet smith.zingiber zerumbet, a perennial rhizomatous herb exhibits remarkable disease resistance as well as a wide range of pharmacological activities. towards characterizing the endophytic population of z. zerumbet rhizomes, experiments were carried out during two different growing seasons viz., early-june of 2013 and late-july of 2014. a total of 34 endophytes were isolated and categorized into 11 morphologically distinct groups. fungi were observed to predominate bacterial species with colonization fre ...201626867602
detection of human pathogenic fusarium species in hospital and communal sink biofilms by using a highly specific monoclonal antibody.the fungus fusarium is well known as a plant pathogen, but has recently emerged as an opportunistic pathogen of humans. habitats providing direct human exposure to infectious propagules are largely unknown, but there is growing evidence that plumbing systems are sources of human pathogenic strains in the fusarium solani species complex (fssc) and fusarium oxysporum species complex (fosc), the most common groups infecting humans. here, a newly developed fusarium-specific monoclonal antibody (mab ...201626914362
identification of the non-ribosomal peptide synthetase responsible for biosynthesis of the potential anti-cancer drug sansalvamide in fusarium solani.sansalvamide is a cyclic pentadepsipeptide produced by fusarium solani and has shown promising results as potential anti-cancer drug. the biosynthetic pathway has until now remained unidentified, but here we used an agrobacterium tumefaciens-mediated transformation (atmt) approach to generate knockout mutants of two candidate non-ribosomal peptide synthetases (nrps29 and nrps30). comparative studies of secondary metabolites in the two deletion mutants and wild type confirmed the absence of sansa ...201626936154
genotyping of fusarium isolates from onychomycoses in colombia: detection of two new species within the fusarium solani species complex and in vitro antifungal susceptibility testing.fusariosis have been increasing in colombia in recent years, but its epidemiology is poorly known. we have morphologically and molecularly characterized 89 isolates of fusarium obtained between 2010 and 2012 in the cities of bogotá and medellín. using a multi-locus sequence analysis of rdna internal transcribed spacer, a fragment of the translation elongation factor 1-alpha (tef-1α) and of the rna-dependent polymerase subunit ii (rpb2) genes, we identified the phylogenetic species and circulatin ...201626943726
novel taxa in the fusarium fujikuroi species complex from pinus spp.the pitch canker pathogen fusarium circinatum has caused devastation to pinus spp. in natural forests and non-natives in commercially managed plantations. this has drawn attention to the potential importance of fusarium species as pathogens of forest trees. in this study, we explored the diversity of fusarium species associated with diseased pinus patula, p. tecunumanii, p. kesiya and p. maximinoi in colombian plantations and nurseries. plants displaying symptoms associated with a f. circinatum- ...201526955193
challenges in fusarium, a trans-kingdom pathogen.fusarium species are emerging human pathogens, next to being plant pathogens. problems with fusarium are in their diagnostics and in their difficult treatment, but also in what are actual fusarium species or rather fusarium-like species. in this issue guevara-suarez et al. (mycopathologia. doi: 10.1007/s11046-016-9983-9 , 2016) characterized 89 isolates of fusarium from colombia showing especially lineages within the fusarium solani and oxysporum species complexes to be responsible for onychomyc ...201626966007
investigation of mycoviruses in endophytic and phytopathogenic strains of colletotrichum from different hosts.fungi belonging to the colletotrichum genus can be categorized as endophytic or phytopathogenic. these fungi can be infected by viruses, termed mycoviruses, which are know to promote hypovirulence in infected fungi. however, there are few studies that have described mycoviral infections of endophytes. the production of secondary metabolites by endophytes with antimicrobial potential in inhibiting numerous pathogens has gained increasing attention. the aim of the current study was to investigate ...201626985921
two new polyhydroxysterols produced by fusarium solani, an endophytic fungus from chloranthus multistachys.a highly antagonistic endophytic fungus, designated strain cl39, was originated from the leaves of chloranthus multistachys collected in wulong of chongqing municipality of china in november 2015. the strain was identified as fusarium solani based on morphological characteristics, 5.8s gene and internal transcribed spacer sequence analysis. two new compounds, 2β, 9α-dihydroxy-5α-methoxyergosta-7, 22-diene (1), 2β, 6β-dihydroxy-5α-methoxyergosta-7, 22-diene (2) have been isolated from the culture ...201627000714
synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents.as part of our continuing research on canthin-6-one antimicrobial agents, a new series of ester derivatives of 10-hydroxycanthin-6-one were synthesized using a simple and effective synthetic route. the structure of each compound was characterized by nmr, esi-ms, ft-ir, uv, and elemental analysis. the antimicrobial activity of these compounds against three phytopathogenic fungi (alternaria solani, fusarium graminearum, and fusarium solani) and four bacteria (bacillus cereus, bacillus subtilis, ra ...201627007362
fusariosis in a patient with acute myeloid leukemia: a case report and review of the literature.fusarium spp. causes infections mostly in patients with prolonged neutropenia. we describe the case of a disseminated fusarium solani infection in a patient with acute myeloid leukemia which never reached complete remission during its clinical course. the patient had profound neutropenia and developed skin nodules and pneumonia in spite of posaconazole prophylaxis. f. solani was isolated from blood and skin biopsy, being identified from its morphology and by molecular methods. by broth dilution ...201627008433
etiology and epidemiological conditions promoting fusarium root rot in sweetpotato.sweetpotato production in the united states is limited by several postharvest diseases, and one of the most common is fusarium root rot. although fusarium solani is believed to be the primary causal agent of disease, numerous other fusarium spp. have been reported to infect sweetpotato. however, the diversity of fusarium spp. infecting sweetpotato in north carolina is unknown. in addition, the lack of labeled and effective fungicides for control of fusarium root rot in sweetpotato creates the ne ...201627050570
epitypification of fusisporium (fusarium) solani and its assignment to a common phylogenetic species in the fusarium solani species complex.fusisporium solani was described as the causal agent of a dry rot of potato in germany in the mid 19th century. as fusarium solani, the species became known as a plurivorous plant pathogen, endophyte, decomposer, and opportunistic pathogen of humans and nutritional symbiont of insects. in parallel, it became evident that the morphologically defined species f. solani represents a phylogenetically and biologically complex group of often morphologically cryptic species that has come to be known in ...201627055573
genotyping and in vitro antifungal susceptibility testing of fusarium isolates from onychomycosis in india.onychomycosis refers to fungal infection of the nail and is commonly caused by dermatophytes, while yeasts and non-dermatophytic molds (ndm) are increasingly recognized as pathogens in nail infections. the present study was done to delineate molecular epidemiology of fusarium onychomycosis in india. five hundred nail samples of indian patients clinically suspected of onychomycosis were subjected to direct microscopy and fungal culture. representative fusarium isolates were further identified to ...201627138574
identification of different fusarium spp. in allium spp. in germany.in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ...201527141742
cytotoxic and antibiotic cyclic pentapeptide from an endophytic aspergillus tamarii of ficus carica.a new cyclic pentapeptide, disulfide cyclo-(leu-val-ile-cys-cys) (1), named malformin e, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus fr02 from the roots of ficus carica. the strain fr02 was identified as aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (its). their structures were determined by the combination of 1d and 2d nmr spectroscopy, hrms (esi), uv, and marfey's an ...201627147299
production of naphthoquinones and phenolics by a novel isolate fusarium solani psc-r of palk bay and their industrial applications.the present study was attempted to enhance the production of naphthoquinones and phenolics by fusarium solani psc-r of palk bay origin, which exhibited potent antibacterial, antioxidant and dyeing activity. maximum productivity of naphthoquinones and phenolics was achieved in potato infusion medium supplemented with 2% sucrose. addition of nitrogen sources to the medium adversely affected the production of both naphthoquinones and phenolics. an initial ph of 5 and incubation at 31°c for six days ...201627156595
diversity, distribution, and antagonistic activities of rhizobacteria of panax notoginseng.rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. this study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of panax notoginseng.201527158229
raman spectroscopy analysis of the biochemical characteristics of experimental keratomycosis.to investigate the biochemical characteristics in experimental keratomycosis by raman spectroscopy analysis in vitro and in vivo.201627158983
time-dependent matrix metalloproteinases and tissue inhibitor of metalloproteinases expression change in fusarium solani keratitis.to investigate matrix metalloproteinases (mmps) and tissue inhibitor of metalloproteinases (timps) expression during the progress of fusarium solani (f.solani) keratitis in a rat model.201627162721
effects of essential oil combinations on pathogenic yeasts and moulds.essential oils (eos) can be used as alternative or complementary antifungal agents against human pathogenic moulds and yeasts. to reduce the effective dose of antimicrobial agents, eos are combined which can lead to synergistic or additive effect. in this study the anti-yeast and anti-mould activities of selected eos were investigated, alone and in combinations, against clinical isolates of candida albicans, c. parapsilosis, aspergillus fumigatus, a. terreus, rhizopus microsporus, fusarium solan ...201627165531
fungal interdigital tinea pedis in dakar (senegal).fungal interdigital tinea pedis (fitp) is the most frequent dermatomycosis in industrial countries. in african tropics, it's a rare motive of consultation and is discovered while complicated. the aims of this article were: to determine the frequency of interdigital tinea pedis among overall mycological analysis in our laboratory; to study epidemiological, clinical and mycological aspects of fitp in outpatients attending the le dantec mycology laboratory in dakar. a total of 62 males (60%) and 42 ...201627184614
reprint of: effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte fusarium solani.volumetric productivity of camptothecin from the suspension culture of the endophyte fusarium solani was enhanced up to ∼152 fold (from 0.19μgl(-1)d(-1) to 28.9μgl(-1)d(-1)) under optimized fermentation conditions including initial ph (6.0), temperature (32°c) and agitation speed (80rpm) with (5% (v/v)) ethanol as medium component. among various elicitors and precursors studied, tryptamine (0.5mm) as precursor and bovine serum albumin (bsa) (0.075mm) as an elicitor added on day 6 of the cultivat ...201627189536
elucidating the key role of the fungal mycelium on the biodegradation of n-pentane as a model hydrophobic voc.the role of the aerial mycelium of the fungus fusarium solani in the biodegradation of n-pentane was evaluated in a continuous fungal bioreactor (fb) to determine the contribution of the aerial (hyphae) and non-aerial (monolayer) fungal biomass. the experimental results showed that although the aerial biomass fraction represented only 25.9(±3)% on a dry weight basis, it was responsible for 71.6(±4)% of n-pentane removal. the fb attained a maximum elimination capacity (ecmax) of 680(±30) g m(-3)  ...201627209557
a case of cutaneous fusariosis of the scrotum as a complication of acute myeloid leukemia.fusarium, a hyphomyocetous fungus, is often isolated from the environment as a laboratory contaminant, but is also known as a pathogen causing keratomycosis, onychomycosis, and opportunistic infection of the skin and viscera. we report a 67-year-old man with localized cutaneous fusariosis of the scrotum, as a complication of acute myeloid leukemia (aml) under chemotherapy. an induration of 25 mm in diameter, which was covered by necrosis and black crust and with pain upon pressure, was found on ...201627251318
[confocal microscopy as an early relapse marker after keratoplasty due to fusarium solani keratitis].in the case of therapy-resistant keratitis an infection with fusarium solani should be taken into consideration as a rare but very severe eye disease. in the majority of cases fusarium solani keratitis will result in a protracted clinical course despite aggressive medicinal and surgical interventions. we describe the case of a referred patient after intensive topical, intracameral and systemic antibacterial and antimycotic therapy as well as surgical treatment with emergency keratoplasty à chaud ...201727260622
endophytic filamentous fungi from a catharanthus roseus: identification and its hydrolytic enzymes.this paper reported on the various filamentous fungi strains that were isolated from a wild grown catharanthus roseus. based on the morphological characteristics and molecular technique through a polymerase chain reaction and dna sequencing method using internal transcribed spacer (its), these fungi had been identified as a colletotrichum sp., macrophomina phaseolina, nigrospora sphaerica and fusarium solani. the ultrastructures of spores and hyphae were observed under a scanning electron micros ...201627275114
therapeutic effect of intrastromal voriconazole, topical voriconazole, and topical natamycin on fusarium keratitis in rabbit.purpose. evaluating the therapeutic effect of topical and intrastromal voriconazole and topical natamycin on fusarium keratitis. methods. 24 rabbits were selected. the stroma of their corneas was inoculated with suspension of fusarium solani species complex. seven days after injection they were divided into 4 groups randomly: the first group was treated with topical voriconazole (tv) 1% for one week, the second one with one-time intrastromal injection of voriconazole (isv) 50 microgram/0.1 ml, a ...201627298734
chitosanase from the plant pathogenic fungus, fusarium solani f. sp. phaseoli-purification and some properties.among 162 strains of the genus fusarium tested, 22 strains, mainly from f. solani and f. splendens, formed halos on chitosan-containing agar medium. chitosanase secreted in the culture by the most effective producer, f. solani f. sp. phaseoli suf386, was further investigated. n-acetylglucosamine (glcnac) used as a carbon source was most effective for production of chitosanase. chitosan, a substrate for chitosanase, inhibited cell growth completely and abolished production of chitosanase when use ...199327314774
rapid identification of moulds and arthroconidial yeasts from positive blood cultures by maldi-tof mass spectrometry.moulds and arthroconidial yeasts are potential life-threatening agents of fungemia in immunocompromised patients. fast and accurate identification (id) of these pathogens hastens initiation of targeted antifungal therapy, thereby improving the patients' prognosis. we describe a new strategy that enabled the identification of moulds and arthroconidial yeasts directly from positive blood cultures by maldi-tof mass spectrometry (ms). positive blood cultures (bcs) with gram staining showing hyphae a ...201627317582
antifungal membranolytic activity of the tyrocidines against filamentous plant fungi.the tyrocidines and analogues are cyclic decapeptides produced by brevibacillus parabrevis with a conserved sequence of cyclo(d-phe(1)-pro(2)-x(3)-x(4)-asn(5)-gln(6)-x(7)-val(8)-x(9)-leu(10)) with trp(3,4)/phe(3,4) in the aromatic dipeptide unit, lys(9)/orn(9) as their cationic residue and tyr (tyrocidines), trp (tryptocidines) or phe (phenicidines) in position 7. previous studies indicated they have a broad antifungal spectrum with the peptides containing a tyr residue in position 7 being more ...201627328781
fast activated charcoal prepurification of fusarium solani β-glucosidase for an efficient oleuropein bioconversion.fungal β-glucosidases were extensively studied regarding their various potential biotechnology applications. here, we report the selection of fusarium solani strain producing high yield of β-glucosidase activity. the effect of some factors on β-glucosidase production was studied including: initial ph, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. the optimal enzyme production was obtained with 4 units of ph. the highest β-glucosidase activ ...201727340756
modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater.the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ...201627348506
fungal associates of the xylosandrus compactus (coleoptera: curculionidae, scolytinae) are spatially segregated on the insect body.studies of symbioses have traditionally focused on explaining one-to-one interactions between organisms. in reality, symbioses are often much more dynamic. they can involve many interacting members, and change depending on context. in studies of the ambrosia symbiosis-the mutualism between wood borer beetles and fungi-two variables have introduced uncertainty when explaining interactions: imprecise symbiont identification, and disregard for anatomical complexity of the insects. the black twig bo ...201627357160
l-asparaginase activity of fungal endophytes from tabernaemontana heyneana wall. (apocynaceae), endemic to the western ghats (india)."endophytes," the microbes residing within the plant tissues, are important sources of secondary metabolites. tabernaemontana heyneana wall., a medicinal tree, endemic to the western ghats with rich ethnobotanical history and unique chemical diversity, was selected to study fungal endophytes and evaluate them for l-asparaginase activity. healthy plant parts were selected for the isolation of endophytes following standard isolation protocols. a total of 727 isolates belonging to 20 taxa were obta ...201427382605
a phenazine-1-carboxylic acid producing polyextremophilic pseudomonas chlororaphis (mcc2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities.the genus pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. in the present study, a psychrotolerant, wide ph tolerant and halotolerant strain of pseudomonas chlororaphis gbpi_507 (mcc2693), isolated from the wheat rhizosphere growing in a mountain location in indian himalayan region (ihr), has been investigated for its antimicrobial potential with particular reference to phenazine pro ...201627394000
antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles.biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.201627400968
genome sequence of fusarium isolate mya-4552 from the midgut of anoplophora glabripennis, an invasive, wood-boring beetle.the fusarium solani species complex (fssc) is a clade of environmentally ubiquitous fungi that includes plant, animal, and insect associates. here, we report the draft genome sequence of the undescribed species fssc 6 (isolate mya-4552), housed in the gut of the wood-boring cerambycid beetle anoplophora glabripennis.201627445364
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