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selected topics from forty years of natural products research: betalains to flavonoids, antiviral proteins, and neurotoxic nonprotein amino acids.the elucidation by nmr and chemical methods of the unique structure of betanidin, the aglycon of the red-violet beet pigment betanin, forty years ago at the university of zürich, switzerland, was the beginning of my plant chemistry research program. many of the same chemical and spectral techniques developed in zürich have been used at the university of texas at austin for the structure analysis of members of many other classes of natural products including especially flavonoids, terpenoids, and ...200111754626
registration of m1-2 beet germplasm resistant to root-knot nematode. 200211756310
enzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.a simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from aspergillus niger. when pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between c-4 and c-5 in the galacturonic acid residue of the released nonreducing end. the formation of double bonds produced an increase in light absorption, which was detected at 235 nm. the assay was tested on pectin of different origins (apple, ...200111767155
survival, root colonisation and biocontrol capacities of pseudomonas fluorescens f113 laczy in dry alginate microbeads.cells of pseudomonas fluorescens f113 laczy were encapsulated in alginate and their survival and ability to colonise sugar beet were evaluated. to assess survival, the formulation, composed of dry alginate microbeads of 300- to 700-microm diameter, was stored 1 year at 28+/-2 and 4+/-2 degrees c and then tested against pathogenic fungi pythium ultimum and rhizoctonia solani in in vitro inhibition experiments. the same material was also used as inoculant for protection of sugar beet against py. u ...200111773997
[anti-cariogenicity of maceration extract of momordica grosvenori: laboratory study].to study the anti-cariogenicity of momordica grosvenori a kind of natural sweet fruit.199811774428
field efficacy and seasonal expression profiles for terminal leaves of single and double bacillus thuringiensis toxin cotton genotypes.examination of commercial cry1ac transgenic bacillus thuringiensis berliner (bt) cotton varieties (bollgard, monsanto, st. louis, mo) and an experimental cry1ac + cry2ab transgenic bt cotton variety (bollgard ii, monsanto) for lepidopteran field efficacy was conducted during the 2000 growing season. in addition, a commercially available (envirologix, portland, me) quantification assay (elisa) was used to measure and profile the expression levels of cry proteins in two of these varieties ['dp50b, ...200111777069
amelioration of nickel phytotoxicity in muck and mineral soils.in situ remediation (phytostabilization) is a cost-effective solution for restoring the productivity of metal-contaminated soils and protection of food chains. a pot experiment with wheat (triticum aestivum l.), oat (avena sativa l.), and redbeet (beta vulgaris l.) was conducted to test the ability of limestone and hydrous ferric oxide (hfo) to ameliorate ni phytotoxicity in two soils contaminated by particulate emissions from a nickel refinery. quarry muck (terric haplohemist; 72% organic matte ...201511790001
the influence of intact-plant and excised-leaf bioassay designs on volicitin- and jasmonic acid-induced sesquiterpene volatile release in zea mays.induced plant responses to insect attack include the release of volatile chemicals. these volatiles are used as host-location signals by foraging parasitoids, which are natural enemies of insect herbivores. a plant's response to herbivory can be influenced by factors present in insect oral secretions. volicitin (n-(17-hydroxylinolenoyl)-l-glutamine), identified in beet armyworm (spodoptera exigua) oral secretions, stimulates volatile release in corn (zea mays l.) seedlings in a manner similar to ...200111800380
complete nucleotide sequence and genome organization of beet soilborne mosaic virus, a proposed member of the genus benyvirus.the complete nucleotide sequences of rnas 1 to 4 of beet soilborne mosaic virus (bsbmv) were determined. the genomic organization of bsbmv is identical to beet necrotic yellow vein virus (bnyvv), the type species of the genus benyvirus. bsbmv rna1 encodes a single large open reading frame (orf) with similar replicase-associated motifs identified for bnyvv. bsbmv rna2 has six potential orfs with an organization resembling bnyvv rna2. rna3 and rna4 resemble the analogous bnyvv rnas, which encode p ...200111811691
biodegradation of lignocellulosic waste by aspergillus terreus.biodegradation of lignocellulosic waste by aspergillus terreus is reported for the first time. this isolate produced 250 cmcase (carboxymethyl cellulase or endoglucanase) u.ml(-1) and biodegraded hay and straw during 3 days and the biomass production on straw was 5g.l(-1) dry weight from 0.25 cm2 inoculated mycellium. this strain secreted endocellulases and exocellulases in the culture medium, but some of the enzymes produced, remained cell membrane bound. cell bound enzymes were released by var ...200111826908
enzymes of the entner-doudoroff and pyruvate decarboxylation pathways in zymomonas mobilis wild-type cp4 and mutant strains grown in continuous culture.the osmotolerant zymomonas mobilis strain suc40, (containing plasmid pds3154-inaz), which is capable of producing simultaneously ethanol and ice nuclei protein, was cultivated in a chemically defined complete sucrose medium, as well as in a sugar beet molasses medium in continuous culture. the strain exhibited the normal monod's relationship between biomass and dilution rate, and between growth substrate concentration and dilution rate. specific activities of a number of enzymes that appear to c ...200111827214
[manifestation of "hairy leaf" trait in mangel beet].new morphological trait, "hairy leaf", of mangel beet has been picked out. dominant character and monogenic inheritance of the trait were found. comparative analysis of manifestation of this trait in mangel and sugar beets was done. it is shown that the gene of "hairiness" (hl) in sugar beet is not allelic to the hlvar gene in mangel beet. this trait can be used as a marker in selection-genetical investigation.200811833338
dual-colour imaging of membrane protein targeting directed by poa semilatent virus movement protein tgbp3 in plant and mammalian cells.the movement function of poa semilatent hordeivirus (pslv) is mediated by the triple gene block (tgb) proteins, of which two, tgbp2 and tgbp3, are membrane proteins. tgbp3 is localized to peripheral bodies in the vicinity of the plasma membrane and is able to re-direct tgbp2 from the endoplasmic reticulum (er) to the peripheral bodies. for imaging of tgbp3-mediated protein targeting, pslv tgbp3 tagged with a red fluorescent protein (dsred) was used. coexpression of dsred-tgbp3 with gfp targeted ...200211842260
colonization of the developing rhizosphere of sugar beet seedlings by potential biocontrol agents applied as seed treatments.poor colonization of the rhizosphere is a major constraint of seed treatment biological control. the objectives of this study were to; examine the colonization of the rhizosphere of sugar beet seedlings by selected rhizobacteria; determine the influence of the host rhizosphere and percolating water on the distribution of the bacteria; and deliver two biological control agents (bcas) by co-inoculation.200211849350
sugar beet mash silage as a component of a total-mixed-ration for dairy cows--effects on parameters of digestion and animal performance.sugar beet mash silage (bms) was offered in amounts up to 35% of dm to dairy cows as a component of a total-mixed-ration (tmr). barley and molasses in the control ration were replaced by bms half in ration bms 1/2 and in total in ration bms 1/1 on the basis of the calculated contents of net energy for lactation. two trials were carried out. in trial i each ration was tested on parameters of rumen fermentation and digestibility of crude nutrients using 4 holstein cows with rumen fistula. chewing ...200111851016
quantitative structure-activity studies of insect growth regulators: xix. effects of substituents on the aromatic moiety of dibenzoylhydrazines on larvicidal activity against the beet armyworm spodoptera exigua.larvicidal activity against the beet armyworm, spodoptera exigua (hübner), was measured after topical treatment for a series of n-tert-butyl-dibenzoylhydrazines having various substituents in the benzoyl (a-ring) moiety closer to the tert-butyl group, the other benzoyl (b-ring) moiety being unsubstituted. the effects of substituents on the larvicidal activity were analyzed using the classical quantitative structure-activity relationship (qsar) procedure. introduction of hydrophobic substituents ...200211852637
[variability of the number of chloroplasts in a population of stoma guard cells in the sugar beet (beta vulgaris l.)].diploid sugar beet plants demonstrate a broad variability of the number of chloroplasts in stoma guard cells, which is related to myxoploidy of cell populations in leaf apical meristems (epigenomic variability). in addition to random organelle segregation between daughter cells, this variability is affected by factors disrupting the mitotic cycle: (1) plant treatment with a mitotic poison, such as colchicine; (2) duration of the life cycle of a plant; the variability in second-year plants is gre ...200211852797
effects of abomasal infusions of histidine, glucose, and leucine on milk production and plasma metabolites of dairy cows fed grass silage diets.our previous study showed that his was the first-limiting amino acid (aa) for milk protein production in cows fed grass silage and cereal-based supplement. the aim of this study was to identify the second-limiting aa and determine whether glucose was limiting responses to his. abomasal infusion of his (6.5 g/d), glucose (250 g/d), his (6.5 g/d) + glucose (250 g/d), his 6.5 g/d) + leu (12 g/d) and his (6.5 g/d) + leu (12 g/d) + glucose (250 g/d) on milk production and utilization of amino acids b ...200211860113
genetically modified feeds in animal nutrition. 2nd communication: glufosinate tolerant sugar beets (roots and silage) and maize grains for ruminants and pigs.to analyse substantial equivalence of genetically modified sugar-beets and maize, in which the glufosinate-tolerant (pat) gene is inserted, crude nutrients, the amino acid and the fatty acid profiles as well as the composition of the ndf-fraction of maize grains were determined and compared with those of the corresponding non-transgenic cultivars. due to the genetic manipulation differences in crude nutrient contents including sugar and starch were not detected. the amino acid profile of maize g ...200111865767
in vivo volatile emissions from peanut plants induced by simultaneous fungal infection and insect damage.peanut plants, arachis hypogaea, infected with white mold. sclerotium rolfsii, emit a blend of organic compounds that differs both quantitatively and qualitatively from the blend emitted from plants damaged by beet armyworm (baw; spodoptera exigua) larvae or from uninfected, undamaged plants. attackby baw induced release of lipoxygenase products (hexenols, hexenals, and hexenyl esters), terpenoids, and indole. the plant-derived compound methyl salicylate and the fungal-derived compound 3-octanon ...200211868672
hydrogen isotopic profile in the characterization of sugars. influence of the metabolic pathway.the site-specific natural hydrogen isotope ratios of plant metabolites determined by 2h nuclear magnetic resonance (snif-nmr method) can provide powerful criteria for inferring mechanistic and environmental effects on biosynthetic pathways. this work examines the potential of isotopic profiles for the main constituents of carbohydrates, glucose and fructose, to distinguish different photosynthetic pathways. an appropriate analytical strategy, involving three suitable isotopic probes, has been el ...200211879039
the provision of solid feeds to veal calves: i. growth performance, forestomach development, and carcass and meat quality.growth performance, forestomach development, and carcass and meat quality of veal calves fed a milk replacer diet (control) were compared to those obtained from calves fed the same liquid diet plus 250 g x calf(-1) x d(-1) of dried beet pulp or wheat straw. three groups of 46 polish friesian calves, balanced according to initial bw, were assigned to the three dietary treatments in a fattening trial, which lasted 160 d. the provision of either solid feed did not affect the milk replacer intake. h ...200211881925
the provision of solid feeds to veal calves: ii. behavior, physiology, and abomasal damage.the aim of this study was to evaluate the effects of the addition of two roughage sources (wheat straw and beet pulp) to the milk replacer diet of veal calves, in order to reduce stress and improve animal welfare. we allocated 138 polish friesian male calves to three different feeding plans: a milk replacer diet (control), 250 g/d of wheat straw in addition to the milk replacer, or 250 g/d of dried beet pulp in addition to the milk replacer. within each feeding treatment, 16 calves were individu ...200211881926
a replication-competent chimera of plant and animal viruses.human, animal, fungal, and plant viruses encode papain-like proteinases that function in polyprotein processing, rna synthesis, and virus-host interactions. to compare the functional profiles of diverse papain-like proteinases, we replaced a proteinase gene of the beet yellows virus (byv) with those derived from equine arteritis virus (eav), foot-and-mouth disease virus (fmdv), and the fungal virus chv1. we found that, although each of the foreign proteinases efficiently processed the viral poly ...200211886267
feeding lambs high-oleate or high-linoleate safflower seeds differentially influences carcass fatty acid composition.our objective was to determine effects of dietary high-oleate (oleate; 76% 18:1) or high-linoleate (linoleate; 78% 18:2) safflower seeds on fatty acids in muscle and adipose tissue of feedlot lambs. white-faced ewe lambs (n = 36) were fed a beet pulp, oat hay, and soybean meal basal diet (control), blocked by bw, and allotted randomly to dietary treatments. cracked safflower seeds were used in isocaloric and isonitrogenous replacement of beet pulp, oat hay, and soybean meal so that oleate and li ...200211890397
production and characterization of pullulan from beet molasses using a nonpigmented strain of aureobasidium pullulans in batch culture.the production of pullulan from beet molasses by a pigment-free strain of aztreobasidium pullulans on shake-flask culture was investigated. combined pretreatment of molasses with sulfuric acid and activated carbon to remove potential fermentation inhibitors present in molasses resulted in a maximum pullulan concentration of 24 g/l, a biomass dry wt of 14 g/l, a pullulan yield of 52.5%, and a sugar utilization of 92% with optimum fermentation conditions (initial sugar concentration of 50 g/l and ...200211900113
identification of fonofos metabolites in latuca sativa, beta vulgaris, and triticum aestivum by packed capillary flow fast atom bombardment tandem mass spectrometry.the metabolism of fonofos, a thiophosphonate insecticide, was investigated in mature lettuce (latuca sativa), beet (beta vulgaris), and wheat (triticum aestivum). six new metabolites were identified by lc-ms and lc-ms-ms analysis using fast atom bombardment (fab) and packed capillary lc columns with application of the on-column focusing technique. these methods provided the sensitivity required to identify unknown metabolites that were present in the mature plants at only 20-230 ppb. structural ...200211902934
metal ions and flexibility in a viral rna pseudoknot at atomic resolution.many pathogenic viruses use programmed -1 ribosomal frameshifting to regulate translation of their structural and enzymatic proteins from polycistronic mrnas. frameshifting is commonly stimulated by a pseudoknot located downstream from a slippery sequence, the latter positioned at the ribosomal a and p sites. we report here the structures of two crystal forms of the frameshifting rna pseudoknot from beet western yellow virus at resolutions of 1.25 and 2.85 a. because of the very high resolution ...200211904368
effect of plant oils and aspartate on rumen fermentation in vitro.the effect of plant oils and aspartate (asp) on rumen fermentation in vitro. the objective of this study was to determine the effect of plant oils (rapeseed - ro, sunflower - so; linseed - lo; 10% wt/wt) and 8 mmol sodium aspartate on rumen fermentation of a diet (250 mg) consisting of hay, barley and sugar beet molasses (60 : 30 : 10). rumen fluid was collected from two slovak merino sheep fed the same diet twice daily. mixed ruminal micro-organisms were incubated in fermentation fluid (40 ml) ...200111906562
adaptation from whitefly to leafhopper transmission of an autonomously replicating nanovirus-like dna component associated with ageratum yellow vein disease.ageratum yellow vein disease is caused by the whitefly-transmitted monopartite begomovirus ageratum yellow vein virus and a dna beta satellite component. naturally occurring symptomatic plants also contain an autonomously replicating nanovirus-like dna 1 component that relies on the begomovirus and dna beta for systemic spread and whitefly transmission but is not required for maintenance of the disease. here, we show that systemic movement of dna 1 occurs in nicotiana benthamiana when co-inocula ...200211907341
glycon specificity profiling of alpha-glucosidases using monodeoxy and mono-o-methyl derivatives of p-nitrophenyl alpha-d-glucopyranoside.hydrolysis of probe substrates, eight possible monodeoxy and mono-o-methyl analogs of p-nitrophenyl alpha-d-glucopyranoside (pnp alpha-d-glc), modified at the c-2, c-3, c-4, and c-6 positions, was studied as part of investigations into the glycon specificities of seven alpha-glucosidases (ec 3.2.1.20) isolated from saccharomyces cerevisiae, bacillus stearothermophilus, honeybee (two enzymes), sugar beet, flint corn, and aspergillus niger. the glucosidases from sugar beet, flint corn, and a. nige ...200211909596
purification and characterization of thermostable endo-1,5-alpha-l-arabinase from a strain of bacillus thermodenitrificans.a strain of a thermophilic bacterium, tentatively designated bacillus thermodenitrificans ts-3, with arabinan-degrading activity was isolated. it produced an endo-arabinase (abn) (ec 3.2.1.99) and two arabinofuranosidases (ec 3.2.1.55) extracellularly when grown at 60 degrees c on a medium containing sugar beet arabinan. the abn (tentatively called an abn-ts) was purified 7,417-fold by anion-exchange, hydrophobic, size exclusion, and hydroxyapatite chromatographies. the molecular mass of abn-ts ...200211916679
[effect of plant preparations on lipid peroxidation parameters in acute toxic hepatitis].the influence of the original vegetable complexes (which include: juices of beet-rout and carrot, decoction of degrose berries, extracts of corn silk, leaves of peppermint and some other components) on the indicators of the cytolysis, lipid peroxidation and antioxidant system of serum of the laboratory rats with acute toxic hepatitis, was investigated. the vegetable complexes exhibited antioxidant effects which were proved by the reduction of the final and intermediate products of lipoperoxidiza ...200111925749
identification of betalains from yellow beet (beta vulgaris l.) and cactus pear [opuntia ficus-indica (l.) mill.] by high-performance liquid chromatography-electrospray ionization mass spectrometry.betaxanthins, the yellow-orange water-soluble pigments from yellow beet (beta vulgaris ssp. vulgaris cv. bejo zaden) and cactus pear (opuntia ficus-indica cv. gialla) have been investigated using an hplc system compatible with mass spectrometry. five novel betaxanthins were found and characterized as the immonium adducts of betalamic acid with serine, gamma-aminobutyric acid, valine, isoleucine, and phenylalanine. to enable concentration of betalain samples, desalting was performed by solid-phas ...200211929288
in situ stabilization of soil lead using phosphorus and manganese oxide: influence of plant growth.in situ stabilization of pb contaminated soils can be accomplished by adding p and mn(iv) oxide. however, the long-term efficacy of in situ stabilization under continual p removal through plant growth is unknown. moreover, the effects these treatments have on phytoavailability of other metals (cd and zn) commonly associated with pb in soil are not well understood. greenhouse experiments using sudax [sorghum vulgare (l.) moench] and swiss chard [beta vulgaris (l.) koch] were carried out to evalua ...201611931448
the aspergillus niger faeb gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds.the faeb gene encoding a second feruloyl esterase from aspergillus niger has been cloned and characterized. it consists of an open reading frame of 1644 bp containing one intron. the gene encodes a protein of 521 amino acids that has sequence similarity to that of an aspergillus oryzae tannase. however, the encoded enzyme, feruloyl esterase b (faeb), does not have tannase activity. comparison of the physical characteristics and substrate specificity of faeb with those of a cinnamoyl esterase fro ...200211931668
production of fructose and ethanol from sugar beet molasses using saccharomyces cerevisiae atcc 36858.the production of enriched fructose syrups and ethanol from beet molasses using saccharomyces cerevisiae atcc 36858 was studied. in batch experiments with a total sugar concentration between 94.9 and 312.4 g/l, the fructose yield was above 93% of the theoretical value. the ethanol yield and volumetric productivity in the beet molasses media with sugar concentration below 276.2 g/l were in the range of 59-76% of theoretical value and between 0.48 and 2.97 g of ethanol/(l x h), respectively. the f ...200211934290
genetic variability of somatic embryogenesis in tissue cultures of sugar beet breeding lines.genetic variability of callus initiation and plant regeneration has been investigated among three sugar beet genotypes. it was found that tdz has a genotype-independent effect on callus initiation and is responsible for more than a two-fold increase in the friable callus induction rate and more than a three-fold increase in the shoot regeneration rate from this callus. along with the genotype-independent organogenesis, regeneration from callus occasionally went through the process of somatic emb ...200811944321
semiquantitative determination of short-chain fatty acids in cane and beet sugars.some sugars, specifically white beet sugar and raw cane sugars, possess off-flavors and off-odors. although not necessarily the source, the presence of short-chain fatty acids serves as an indicator of an off-odor problem in sugar. solid-phase microextraction (spme) is used to collect the volatile compounds from the headspace of sugar. the temperature, moisture, and type of spme fiber are varied to optimize recovery. sugars analyzed in the absence of water using an incubation temperature of 70 d ...200211954649
enzymatic hydrolysis of ammonia-treated sugar beet pulp.sugar beet pulp is a carbohydrate-rich coproduct generated by the table sugar industry. beet pulp has shown promise as a feedstock for ethanol production using enzymes to hydrolyze polymeric carbohydrates and engineered bacteria to ferment sugars to ethanol. in this study, sugar beet pulp underwent an ammonia pressurization depressurization (apd) pretreatment in which the pulp was exploded by the sudden evaporation of ammonia in a reactor vessel. apd was found to substantially increase hydrolysi ...200111963856
production of bacterial cellulose from alternate feedstocks.production of bacterial cellulose by acetobacter xylinum atcc 10821 and 23770 in static cultures was tested from unamended food process effluents. effluents included low-solids (ls) and high-solids (hs) potato effluents, cheese whey permeate (cw), or sugar beet raffinate (csb). strain 23770 produced 10% less cellulose from glucose than did strain 10821 and diverted more glucose to gluconate. unamended hs, cw, and csb were unsuitable for cellulose production by either strain, and ls was unsuitabl ...200111963879
guard cell wall: immunocytochemical detection of polysaccharide components.the composition of guard cell walls in sugar beet leaves (beta vulgaris l.) was studied by using histochemical staining and immunocytochemical detection of cell wall antigens. the findings were compared with those in the walls of epidermal and mesophyll cells. probing of leaf sections with monoclonal antibodies against pectins, terminal fucosyl residues linked alpha-(1-->2) to galactose, beta-(1-->3)-glucans and arabinogalactan-proteins revealed several specific features of guard cells. pectic e ...200211971918
effects of different fibre sources and fat addition on cholesterol and cholesterol-related lipids in blood serum, bile and body tissues of growing pigs.knowledge is limited on the efficacy of hindgut-fermentable dietary fibre to reduce blood, bile and body tissue cholesterol levels. in three experiments with growing pigs the effects of different kinds and levels of bacterially fermentable fibre (bfs) on cholesterol metabolism were examined. various diets calculated to have similar contents of metabolizable energy were supplied for complete fattening periods. in the first experiment, a stepwise increase from 12 to 20% bfs was performed by supple ...200211972674
analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (orac) and ferric reducing antioxidant power (frap) assays: a comparative study.a total of 927 freeze-dried vegetable samples, including 111 white cabbages, 59 carrots, 51 snap beans, 57 cauliflower, 33 white onions, 48 purple onions, 130 broccoli, 169 tomatoes, 25 beets, 88 peas, 88 spinach, 18 red peppers, and 50 green peppers, were analyzed using the oxygen radical absorption capacity (orac) and ferric reducing antioxidant capacity (frap) methods. the data show that the orac and frap values of vegetable are not only dependent on species, but also highly dependent on geog ...200212009973
biological control of cucumber and sugar beet damping-off caused by pythium ultimum with bacterial and fungal antagonists.five bacterial strains belonging to bacillus subtilis, pseudomonas fluorescens and ps. corrugata and two fungal strains belonging to trichoderma viride and gliocladium virens were evaluated for their efficacy in controlling sugar beet and cucumber damping-off caused by pythium ultimum.200212010548
identification of a tolerant locus on arabidopsis thaliana to hypervirulent beet curly top virus cfh strain.the infection of hosts by the geminivirus depends on the interactions between host and viral factors for viral dna replication, viral gene expression, and the movement of virus throughout the hosts. this work reports that a hypervirulent strain of beet curly top virus (bctv) is different in its ability to infect several ecotypes of arabidopsis thaliana. symptoms appeared on arabidopsis ecotypes around 7 to 10 d after inoculation with bctv-cfh. symptoms were more severe in ecotype skku including ...200212018847
effects of virus concentration and ultraviolet irradiation on the activity of corn earworm and beet armyworm (lepidoptera: noctuidae) nucleopolyhedroviruses.laboratory studies were initiated to determine the relationship between virus concentration and radiation-caused inactivation of npvs from helicoverpa zea (hzsnpv) and spodoptera exigua (semnpv). in the laboratory, a uv-b/uv-a system was used for inactivation studies. for both viruses inactivation was dependent upon both length of uv exposure and virus concentration. at all virus concentrations hzsnpv was more sensitive to uv than semnpv. in the field hzsnpv was used and virus persistence was si ...200212019996
relative effects of ultraviolet and visible light on the activities of corn earworm and beet armyworm (lepidoptera: noctuidae) nucleopolyhedroviruses.five different combinations of fluorescent tubes (uv-b/uv-b, uv-b/uv-a, uv-a/ uv-a, uv-b/white, white/white) were used to determine relative effects of uv and visible light on the nucleopolyhedroviruses (npv) of helicoverpa zea and spodoptera exigua. for both viruses, the greatest inactivation occurred with exposure to uv-b radiation. both virus concentration and radiation exposure time influenced the rate and degree of inactivation. in the case of the uv-a/uv-a and white/white combinations inac ...200212019999
pro-active management of beet armyworm (lepidoptera: noctuidae) resistance to tebufenozide and methoxyfenozide: baseline monitoring, risk assessment, and isolation of resistance.susceptibility to tebufenozide and methoxyfenozide of beet armyworm [spodoptera exigua (hübner)] from the southern united states and thailand was determined through exposure of first and third instars to dipped cotton leaves. among the field populations evaluated, tebufenozide lc50 values for first and third instars, respectively, ranged from 0.377 to 4.41 and 4.37-46.6 microg (ai) /ml of solution. methoxyfenozide lc50 values for first and third instars of field populations ranged from 0.058 to ...200212020022
evaluation of peracid activated organophosphates in studies of insecticide resistance conferred by insensitive acetylcholinesterases.peracid activated organophosphorus (op) insecticides were evaluated against electric eel and a range of insect acetylcholinesterases (ache). incubation of chlorpyrifos and sulprofos with meta-chloroperoxybenzoic acid (mcpba) produced active metabolites capable of distinguishing between ache enzymes conferring op susceptibility and resistance in populations of the tobacco whitefly, bemisia tabaci gennadius, and the beet armyworm, spodoptera exigua hubner. these products were also active against e ...200212020023
isolation and molecular analysis of six taproot expressed genes from sugar beet.the taproot from sugar beet (beta vulgaris l.) undergoes a specific developmental process to function as a food storage organ. suppression subtractive hybridization (ssh) was utilized for the isolation of cdna fragments for taproot expressed genes. isolation and molecular analysis of six cdnas encoding the complete gene product revealed that these genes comprise homologues of a drought-inducible linker histone, a homologue of a major latex-like protein, a phosphoenolpyruvate carboxylase kinase, ...200212021303
pseudomonas fluorescens dr54 reduces sclerotia formation, biomass development, and disease incidence of rhizoctonia solani causing damping-off in sugar beet.effects of the biocontrol strain, pseudomonas fluorescens dr54, on growth and disease development by rhizoctonia solani causing damping-off in sugar beet were studied in soil microcosms and in pot experiments with natural, clay-type soil. in pot experiments with p. fluorescens dr54-treated seeds, significantly fewer rhizoctonia-challenged seedlings showed damping-off symptoms than when not inoculated with the biocontrol agent. in the rhizosphere of p. fluorescens dr54 inoculated seeds, the bacte ...200112024268
[hepatoprotective effect of plant preparations].the hepatoprotector activity of original compositions of plant origin, containing beet and carrot juices, decoction of dog rose fruits, and extracts of corn silk, peppermint leaves, and common horsetail herbs was studied on an acute hepatitis model induced by tetrachloromethane. an analysis of the data on the hepatocyte cytolysis, cholestasis, lipid peroxidation, and antioxidant system of blood serum showed that the preparations possess membranoprotector and antioxidant properties. this was mani ...200212025784
adsorption of several metal ions onto a low-cost biosorbent: kinetic and equilibrium studies.sugar beet pulp generated by sugar-refining factories has been shown to be an effective adsorbent for the removal of heavy metals from aqueous solutions. the structural components related to the metallic adsorption being determined, batch adsorption studies were performed for several metal ions, namely, pb2+, cu2+, zn2+, cd2+, and ni2+ cations. two simple kinetic models, that is, pseudo-first- and pseudo-second-order, were tested to investigate the adsorption mechanisms. the kinetic parameters o ...200212026994
glycine betaine accumulation, ionic and water relations of red-beet at contrasting levels of sodium supply.exposure of plants to sodium (na) and salinity may increase glycine betaine accumulation in tissues. to study this, red-beet cvs. scarlet supreme and ruby queen, were grown for 42 days in a growth chamber using a re-circulating nutrient film technique with 0.25 mmol/l k and either 4.75 mmol/l (control) or 54.75 mmol/l (saline) na (as nacl). plants were harvested at weekly intervals and measurements were taken on leaf water relations, leaf photosynthetic rates, chlorophyll fluorescence, chlorophy ...200112033231
identification of the subgenomic mrnas that encode 6-kda movement protein and hsp70 homolog of beet yellows virus.a tandem arrangement of the genes encoding the approximately 6-kda hydrophobic protein (p6) and hsp70 homolog (hsp70h) is conserved among the members of the closterovirus genus. it was not known, however, if these movement proteins are expressed from one or two subgenomic (sg) rnas. here we employ rna ligase-mediated race to show that the beet yellows virus (byv), a prototype closterovius, produces separate sgrnas encoding p6 and hsp70h. this result is further supported by generation of the reco ...200212033789
[dna degradation in the leaves of the sugar beet (beta vulgaris l.) in the process of senescence].programmed cell death (pcd) is a characteristic of all living beings in process of their development and in response to biotic and abiotic factors effects. on the pattern of the growing old 7-8th leaves of the sugar-beet (beta vulgaris l.) of the first year vegetation of belocerkovskaya singl-seed-45 sort character of the degradation and state of the methylation gtcgac- and ctgcag-sequences of the dna were investigated. it was established by a method of electrophoresis that in the growing old le ...201512035541
new medium composition for high betacyanin production by a cell suspension culture of table beet (beta vulgaris l.).the effect of a revised linsmaier-skoog (ls) medium on betacyanin production was investigated in suspension cultures of table beet (beta vulgaris l.). the effects of a high iron concentration and low concentration of zinc on betacyanin production were not cumulative. the composition of the new revised medium for high betacyanin production was established by reducing the concentration of inorganic nitrogen (30 mm), modifying the ratio of ammonium to nitrate (1:14), reducing the concentration of z ...200212036073
biological properties and molecular characterization of beet chlorosis virus (bchv).two distinct viruses belonging to the polerovirus genus, in the family luteoviridae, have been described as being able to induce mild yellowing on sugar beet: beet mild yellowing virus (bmyv) and more recently, beet chlorosis virus (bchv). we have analysed biological properties and molecular organisation of two strains of bchv, one collected in england and the second from california. the biological data suggested that bchv displayed a narrower host range compared to bmyv and beet western yellows ...200212038685
modeling of single and competitive metal adsorption onto a natural polysaccharide.sugar beet pulp, a common agricultural waste, was studied in the removal of metal ions from aqueous solutions. potentiometric titrations were used to characterize the surface acidity of the polysaccharide. the acid properties of the material can be described by invoking three distinct types of surface functional groups with the intrinsic acidity constants (pka(int)) values 3.43+/-0.1, 6.05+/-0.05, and 7.89+/-0.1, respectively. the contents of each functional group (i.e., the carboxyl and phenol ...200212038836
expression of apolipoprotein a-i mrna in liver and intestine of cecectomized rats fed beet fiber.sham-operated and cecectomized rats were fed a cholesterol-free diet with no added fiber (fiber-free) for 10 days, followed by the diet of 15% beet fiber for 10 days. the plasma cholesterol concentrations in rats fed the fiber-free diet were not significantly different between sham-operated and cecectomized groups. plasma cholesterol concentrations in sham-operated rats were decreased by feeding the beet fiber diet, whereas those in cecectomized rats did not change. final plasma total cholestero ...199512049999
p0 of beet western yellows virus is a suppressor of posttranscriptional gene silencing.higher plants employ a homology-dependent rna-degradation system known as posttranscriptional gene silencing (ptgs) as a defense against virus infection. several plant viruses are known to encode proteins that can suppress ptgs. here we show that p0 of beet western yellows virus (bwyv) displays strong silencing suppressor activity in a transient expression assay based upon its ability to inhibit ptgs of green fluorescent protein (gfp) when expressed in agro-infiltrated leaves of nicotiana bentha ...200212050394
evaluation of process-induced dimensional changes in the membrane structure of biological cells using impedance measurement.the impact of high intensity electric field pulses, high hydrostatic pressure, and freezing-thawing on local structural changes of the membrane was determined for potato, sugar beet tissue, and yeast suspensions. on the basis of the electrophysical model of cell systems in biological tissues and suspensions, a method was derived for determining the extent of local damage of cell membranes. the method was characterized by an accurate and rapid on-line determination of frequency-dependent electric ...200212052078
effect of plant oils and organic acids on rumen fermentation in vitro.we determined the effect of plant oils (rapeseed, sunflower, linseed) and organic acids (aspartic and malic) on the fermentation of diet consisting of hay, barley and sugar beet molasses. rumen fluid was collected from two sheep (slovak merino) fed with the same diet twice daily. mixed rumen microorganisms were incubated in fermentation fluid, which contained rumen fluid and mc dougall's buffer. all supplemented diets significantly increased ph, molar proportion of propionate, and numerically de ...200212058397
digestibility and metabolic utilisation of dietary energy in adult sows: influence of addition and origin of dietary fibre.according to a 4 x 4 latin square design, four adult ovariectomised sows fed at a similar energy level (516 kj me/kg body weight (bw)0.75 per d) received one of four diets successively: a control low-dietary-fibre (df) diet (diet c, 100 g total df/kg dm) and three fibre-rich diets (200g total df/kg dm) that corresponded to a combination of diet c and maize bran (diet mb), wheat bran (diet wb), or sugar-beet pulp (diet sbp). sows were adapted to the diet for 12 d before an 8 d measurement period. ...200212064342
[expression of cms in zygotic and apozygotic progenies of sugar beet beta vulgaris l].zygotic and apozygotic progenies of sugar beet exhibit high phenotypic variation with respect to cytoplasmic male sterility (cms). there are progenies with completely sterile, semisterile, semifertile, and fertile pollen. the proportions of semifertile and fertile plants in zygotic and apozygotic progenies varied from zero to 28% and from zero to 17.8%, respectively. comparison of the phenotypic distributions in zygotic and apozygotic progenies did not reveal significant differences in the cms e ...200212068549
[production of regenerants in sugar beet].the experimental results are presented on production of plants-regenerants of the sugar beet (beta vulgaris l.) via callus formation or direct organogenesis from the leaf tissues. the method of aseptic treatment was developed for the seeds with strong bacterial and fungal invasion. the regenerant plants were obtained in the presence of various concentrations of synthetic hormones, such as cytokinin (6-benzaminopurine) and auxin (naphthylacetic acid), and inhibitor of auxin transport in plants (2 ...201612068716
the presence of lewis a epitopes in arabidopsis thaliana glycoconjugates depends on an active alpha4-fucosyltransferase gene.the presence of an alpha4-fucosyltransferase in plants was first deduced from the characterization of lewis-a glycoepitopes in some n-glycans. the first plant gene encoding an alpha4-fucosyltransferase was recently cloned in beta vulgaris. in the present paper we provide evidence for the presence of an alpha4-fucosyltransferase in a. thaliana by measurement of this glycosyltransferase activity from a purified microsomal preparation and by immunolocalization of le(a) epitopes on glycans n-linked ...200212070072
increased production of nutriments by genetically engineered crops.plants are the basis of human nutrition and have been selected and improved to assure this purpose. nowadays, new technologies such as genetic engineering and genomics approaches allow further improvement of plants. we describe here three examples for which these techniques have been employed. we introduced the first enzyme involved in fructan synthesis, the sucrose sucrose fructosyltransferase (isolated from jerusalem artichoke), into sugar beet. the transgenic sugar beet showed a dramatic chan ...200212071305
detection of gene-anchored amplification polymorphism (gaap) in the vicinity of plant mitochondrial genes.a simple, semi-automatable method was established for assessing polymorphism in plant mitochondrial genome. a set of 41 mitochondrial markers based on the published arabidopsis thaliana sequence was developed in brassicaceae using a gene-anchored amplification polymorphism (gaap) strategy. pcr primers were selected based on conserved coding regions of mitochondrial genes and used to amplify the corresponding 5' and/or 3' non-coding flanking regions in order to maximise sequence variability betwe ...200212073035
characterization of fecal bacterial populations in canines: effects of age, breed and dietary fiber.the effects of age, breed, and diet on fecal chemistry, enzyme activity, and bacterial populations of dogs were studied. eighteen dogs from two age groups (young: 2.5 +/- 0.5 years, old: 10.9 +/-0.7 years) and three different breeds (german shepherds, miniature schnauzers, and english setters) were rotated through a latin square design such that every dog was fed each of the diets. the test diets included a low-fiber (control) diet and a 10% fiber diet which contained 5% soybean hulls and 5% bee ...200212087428
reduced ammonia emissions from slurry after self-acidification with organic supplements.ammonia volatilisation from field applied slurries causes environmental hazards and loss of fertilizer value. acidification of slurry, usually with inorganic or organic acids has previously been used to reduce nh3 emissions. in this study, we present an alternative technique for the acidification of slurry, namely the use of fermentation by endogenous microbes to form organic acids from readily degradable organic compounds. in laboratory experiments, the addition of different sugars (sucrose in ...200212088370
antibiotic and biosurfactant properties of cyclic lipopeptides produced by fluorescent pseudomonas spp. from the sugar beet rhizosphere.cyclic lipopeptides (clps) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent pseudomonas spp. to provide new and efficient strains for the biological control of root-pathogenic fungi in agricultural crops, we isolated approximately 600 fluorescent pseudomonas spp. from two different agricultural soils by using three different growth media. clp production was observed in a large proportion of the strains (approximately 60%) inhabiting th ...200212089023
energy metabolism of cocks and broiler chickens fed on diets with different carbohydrate sources.energy balances of cocks and chickens were measured using the nitrogen-carbon-balance method. in experiment 1 twelve adult white leghorn cocks were fed alternately on a basal ration or on a supplemental ration composed of 75% basal diet and 25% carbohydrate source as a supplement. in experiment 2 six groups of 12 male broiler chickens were fed successively on two diets each with different carbohydrate sources (40% of dm) and on two energy levels. the investigated carbohydrate sources were glucos ...200112098835
[comparison of productivity of different vitamin green technologies under the space station conditions].at present, fresh plant products for nutrition of the international space station (iss) crews are delivered from earth in small quantities. regular supply of additional fresh greens could be positive for improvement as of nutrition, so psychophysical state of iss crews. vitamin greens can be produced with the use of various technologies: planting leaf cultures in greenhouses, forcing the greens from onions and root vegetables (onion, garlic, chicory, beet, parsley etc.), and germinating seeds. p ...200212098946
painting of parental chromatin in beta hybrids by multi-colour fluorescent in situ hybridization.sugar beet (beta vulgaris l.) is a relatively young crop and has a narrow gene pool. in order to introduce genetic variability into the crop, interspecific hybrids, selected from crosses with wild beets of the sections corollinae and procumbentes, have been generated. the introgressed b. procumbens chromatin carries resistance genes to beet cyst nematode heterodera schachtii schm. these lines are important for breeding of nematode-resistant sugar beet, while corollinae species are potential dono ...200212099348
genotypic variation for drought tolerance in beta vulgaris.insufficient soil moisture during summer months is now the major cause of sugar beet yield losses in the uk. however, selection for increased drought tolerance has not been a breeding priority until recently. genetic variation for drought tolerance is an essential prerequisite for the development of more stress-tolerant varieties, but commercial sugar beet varieties seem to have similar yield responses to drought. the objective of this study was to assess the degree of genotypic variation for dr ...200212102517
influence of the pattern of peptide supply on microbial activity in the rumen simulating fermenter (rusitec).the source and pattern of n supply was varied in the rumen simulation technique (rusitec) in order to determine if continuous, rather than transient, availability of peptides was required for optimum ruminal fermentation. the energy source was fibre prepared from sugar-beet pulp. n was added as nh3 continuously infused (ac) or peptides (bacto(r) casitone, a pancreatic hydrolysate of casein; difco laboratories, detroit, mi, usa) continuously infused (pc) or added as a single dose at the time of f ...200212117430
determination of degradation of radioactivity and its kinetics in aerobic composting.in this study, the kinetics of disappearance of radioactivity in aerobic composting was investigated. for this purpose, compost materials were prepared by mixing sugar beet wastes, wine factory wastes (grape wastes), straw and biological treatment sludge in different amounts. while alpha-radioactivity was not initially detected in all composting materials, the composting materials had some beta-radioactivity. in the mixtures of sugar beet wastes--straw-biological treatment sludge (1), sugar beet ...200212118707
an improved method for whole-mount in situ hybridization of heterodera schachtii juveniles.an optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, heterodera schachtii, by whole-mount in situ hybridization. two different probes were used for genes with known expression pattern in other nematodes. vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. additional modifications were introduced to simplify and standardize the process.200212122430
heavy metals and arsenic uptake by wild vegetation in the guadiamar river area after the toxic spill of the aznalcóllar mine.on 25 april 1998, approximately 4.5 hm(3) of pyritic sludge, containing 5000 mg of as kg(-1) among other pollutants, was spilled into the agrio and guadiamar rivers and the surrounding agricultural areas (aznalcóllar, seville, southern spain). many trace metals such as pb, cu, zn, cd, tl, sb and as reached the doñana national park, the largest wetland area in europe, affecting soils, different plant and animal species. in order to recuperate the affected lands by employing plants capable of accu ...200212126811
effect of enhanced concentration of co2 on transpiration and kinetic parameters of photosynthetic co2 gas exchange in sugar beet grown at different concentrations of nitrate. 201612134499
attempts at improving citric acid fermentation by aspergillus niger in beet-molasses medium.natural oils with high unsaturated fatty acids content when added at concentrations of 2% and 4% (v/v) to beet molasses (bm) medium caused a considerable increase in citric acid yield from aspergillus niger. the fermentation capacities were also examined for production of citric acid using bm-oil media under different fermentation conditions. maximum citric acid yield was achieved in surface culture in the presence of 4% olive oil after 12 days incubation.200212137276
trace level determination of organophosphorus pesticides in water with the new direct-electron ionization lc/ms interface.a new lc/ms method for the determination of organophosphorus pesticides in water, based on the use of direct-electron ionization (ei) interface, is presented. direct-ei is a new device that, in a very simple fashion, couples a nano-hplc system with a mass spectrometer equipped with electron ionization capability. the nanoscale liquid flow allows for a direct introduction of eluate into the ion source and, after nebulization, for its ionization under typical ei conditions. library-matchable ei sp ...200212139067
sucrose-mediated transcriptional regulation of sucrose symporter activity in the phloem.a proton-sucrose symporter mediates the key step in carbon export from leaves of most plants. sucrose transport activity and steady-state mrna levels of bvsut1, a sugar beet leaf sucrose symporter, are negatively regulated specifically by sucrose. results reported here show that bvsut1 mrna was localized to companion cells of the leaf's vascular system, which supports its role in the systemic distribution of photoassimilate. immunoblot analysis showed that decreased transport activity was caused ...200212149483
protoplasmic ph modifies water and solute transfer in beta vulgaris root vacuoles.volume changes were studied in beta vulgaris storage root vacuoles, using video microscopy, when exposed to hypotonic conditions. the osmotic gradient was either step-applied or progressively imposed in perfusion experiments. preincubation at low ph (6.6) or with hgcl2 strongly reduced the vacuoles' water permeability, measured in step experiments. furthermore, the volumetric response depended on the rate with which the aniso-osmotic condition was established. in perfusion experiments a "plateau ...200212163976
the short-term feeding behavior of growing pigs fed foods differing in bulk content.we investigated the effects of foods of different bulk on the short-term feeding behavior (stfb) of 16 individually housed pigs. the three foods used had different bulk contents [low-control (c), medium-70% wheat bran (wb), and high-70% sugar beet pulp (sbp)]. we expected the different intakes of the foods to be reflected in differences in stfb. three hypotheses were developed based on ideas about the way in which a physical constraint to intake could arise. h(1): there would be less diurnal var ...200212175596
the phytotoxic lipodepsipeptide syringopeptin 25a from pseudomonas syringae pv syringae forms ion channels in sugar beet vacuoles.syringopeptin 25a (sp(25)a) belongs to a family of cyclic lipodepsipeptides (ldps) produced by the gram-negative bacterium pseudomonas syringae, a phytopathogenic organism that affects several plants of agronomic interest. ldps increase the permeability of plasma and, possibly, intracellular membranes in plant cells. consistently, sp(25)a forms ion channels in planar lipid bilayers and other model membranes. here we used sugar beet tonoplasts as a new biological model system to study toxin actio ...200212181614
virus-induced gene silencing in transgenic plants expressing the minor capsid protein of beet western yellows virus.transgenic nicotiana benthamiana expressing the minor coat protein p74 of the phloem-limited beet western yellows virus (bwyv) exhibited an unusual spatial pattern of post-transcriptional gene silencing (ptgs) when infected with bwyv or related viruses. following infection, transgenic p74 and its mrna accumulated to only low levels, 21 to 23 nucleotide rnas homologous to the transgene appeared, and the transgene dna underwent methylation. the infecting viral rna, however, was not subject to sign ...200212182337
thermodynamic analysis of conserved loop-stem interactions in p1-p2 frameshifting rna pseudoknots from plant luteoviridae.the rna genomes of plant luteovirids beet western yellows virus (bwyv), potato leaf roll virus (plrv), and pea enation mosaic virus (pemv rna1; pemv-1) contain a short mrna pseudoknotted motif overlapping the p1 and p2 open reading frames required for programmed -1 mrna ribosomal frameshifting. the relationship between structure, stability, and function is poorly understood in these rna systems. a m(5)-c(8)-substituted bwyv rna is employed to establish that the bwyv p1-p2 pseudoknot is protonate ...200212186552
lipopeptide production in pseudomonas sp. strain dss73 is regulated by components of sugar beet seed exudate via the gac two-component regulatory system.pseudomonas sp. strain dss73 isolated from the sugar beet rhizosphere produces the cyclic lipopeptide amphisin, which inhibits the growth of plant-pathogenic fungi. by tn5::luxab mutagenesis, we obtained two nonproducing mutant strains, dss73-15c2 and dss73-12h8. the gene interrupted by the transposon in strain dss73-15c2 (amsy) encoded a protein with homology to peptide synthetases that was designated amphisin synthetase. dss73-12h8 carried the transposon in a regulatory gene encoding a protein ...200212200307
[risk factors for wilms' tumor development in children].wilms' tumor (wt) is the most frequent embryonic renal neoplasia in children.200912200874
relationship between content of crude protein in rations for dairy cows and milk yield, concentration of urea in milk and ammonia emissions.during recent decades, efforts have been made in several countries to diminish the negative environmental influence of dairy production. the main focus has been on nitrogen and phosphorus. modern dairy production in western europe is often based on imported feed-stuffs, mostly protein-rich feeds. in sweden at least, it is wished that the use of imported feedstuffs in animal production will decrease due to the risk of contamination with salmonella and the ban of using gmo crops in swedish dairy p ...200212201534
transgenically expressed betaine aldehyde dehydrogenase efficiently catalyzes oxidation of dimethylsulfoniopropionaldehyde and [omega]-aminoaldehydes.tobacco (nicotianum tabacum l.) plants engineered to express a sugar beet (beta vulgaris l.) betaine aldehyde dehydrogenase (badh) cdna acquired not only badh activity, but also three other aldehyde dehydrogenase activities (those measured with 3-dimethylsulfoniopropionaldehyde, 3-aminopropionaldehyde, and 4-aminobutyraldehyde, all of which are natural products). this shows that badh is not, as believed up to now, a substrate-specific enzyme and that its role may not be limited to glycine betain ...199712223684
de novo biosynthesis of volatiles induced by insect herbivory in cotton plants.in response to insect feeding on the leaves, cotton (gossypium hirsutum l.) plants release elevated levels of volatiles, which can serve as a chemical signal that attracts natural enemies of the herbivore to the damaged plant. pulse-labeling experiments with [13c]co2 demonstrated that many of the volatiles released, including the acyclic terpenes (e,e)-[alpha]-farnesene, (e)-[beta]-farnesene, (e)-[beta]-ocimene, linalool, (e)-4,8-dimethyl-1,3,7-nonatriene, and (e/e)-4,8,12-trimethyl-1,3,7,11-tri ...199712223763
effects of prolonged washing on primary and secondary transport processes at the plasma membrane in red beet storage tissue.changing patterns of enzyme activity and solute transport in response to washing were investigated in red beet (beta vulgaris l.) storage tissue. washing had a pronounced effect on the plasma membrane (pm) h+-atpase with an increase in both hydrolytic and proton-pumping activities. immunoblotting indicated that this may be due, in part, to a higher amount of this enzyme in the pm of washed tissue. activities of the tonoplast (v)h+-atpase and pyrophosphatase fluctuated during a 4-d washing period ...199712223806
synthesis of the (17r)- and (17s)-isomers of volicitin, an elicitor of plant volatiles contained in the oral secretion of the beet armyworm.both the (17r)- and (17s)-isomers of volicitin, which is contained in the oral secretion of the beet armyworm and induces corn seedlings to emit a blend of volatile compounds to attract the natural enemy of the herbivore, were synthesized via the semi-hydrogenation of an intermediary diyne and (z)-selective olefination as the key steps. they were both obtained as crystalline compounds.200212224650
solution structure of a luteoviral p1-p2 frameshifting mrna pseudoknot.a hairpin-type messenger rna pseudoknot from pea enation mosaic virus rna1 (pemv-1) regulates the efficiency of programmed -1 ribosomal frameshifting. the solution structure and 15n relaxation rates reveal that the pemv-1 pseudoknot is a compact-folded structure composed almost entirely of rna triple helix. a three nucleotide reverse turn in loop 1 positions a protonated cytidine, c(10), in the correct orientation to form an a((n-1)).c(+).g-c(n) major groove base quadruple, like that found in th ...200212225754
the kinetics of n-ethylmaleimide inhibition of a vacuolar h+-atpase and determination of nucleotide dissociation constants.all eukaryotic vacuolar (v-type) atpases share the property of being inhibited by low concentrations (1-2 [mu]m) if n-ethylmaleimide (nem). this distinguishes them from p-type atpases, which are inhibited by higher concentrations of nem (0.1-1 mm), and f-type atpases, which are virtually resistant to inhibition by nem. using tonoplast vesicles from beta vulgaris we have determined the kinetics of nem inactivation of the v-type atpase to be pseudo-first order. the concentration dependence of the ...199612226173
the ph requirement for in vivo activity of the iron-deficiency-induced "turbo" ferric chelate reductase (a comparison of the iron-deficiency-induced iron reductase activities of intact plants and isolated plasma membrane fractions in sugar beet).the characteristics of the fe reduction mechanisms induced by fe deficiency have been studied in intact plants of beta vulgaris and in purified plasma membrane vesicles from the same plants. in fe-deficient plants the in vivo fe(iii)-ethylenediaminetetraacetic complex [fe(iii)-edta] reductase activity increased over the control values 10 to 20 times when assayed at a ph of 6.0 or below ("turbo" reductase) but increased only 2 to 4 times when assayed at a ph of 6.5 or above. the fe(iii)-edta redu ...199612226175
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