Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| a single nucleotide polymorphism and sequence analysis of csn1s1 gene promoter region in chinese bos grunniens (yak). | the aim of this study was to investigate the polymorphism of the csn1s1 gene promoter region in 4 chinese yak breeds, and compare the yak csn1s1 gene promoter region sequences with other ruminants. a polymerase chain reaction-single strand conformation polymorphism protocol was developed for rapid genotyping of the yak csn1s1 gene. one hundred fifty-eight animals from 4 chinese yak breeds were genotyped at the csn1s1 locus using the protocol developed. a single nucleotide polymorphism of the csn ... | 2010 | 20024785 |
| buffalo (bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens. | in this study, inner cell mass (icm) cells were isolated from in vitro produced buffalo blastocysts and were cultured on mitomycin-c treated buffalo foetal fibroblast feeder layer for producing embryonic stem (es) cells. among different sources (hatched vs expanded blastocysts) or methods (enzymatic vs mechanical), mechanical isolation of icm from hatched blastocysts resulted in the highest primary colony formation rate and the maximum passage number up to which es cells survived. putative es ce ... | 2011 | 20042025 |
| [first notification of eimeria bareillyi (apicomplexa: eimeridae) in feces of buffalo calves (bubalus bubalis) naturally infected in minas gerais, brazil]. | in this study the evolution of eimeriosis in naturally infected calves was analyzed during 23 days starting at birth. the specie e. bareillyi was first identified in feces culture. later other species of eimeria already described in water buffalo were identified. the animals which died were submitted to necropsy, revealing macroscopic lesions in the ileum region. histological analyses of the ileum region showed acute necrotic enteritis and the presence of eimeria sp. in different developmental s ... | 2008 | 20059855 |
| detection of follicular apoptosis in water buffalo (bubalus bubalis) ovary by histology and nick end labelling technique. | the objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (bubalus bubalis) ovary using classical histology and nick end labelling technique. ovaries (n=40) procured from the slaughterhouse were used for the study. the sections (5 μm) were used for detection of terminal deoxynucleotidyl transferase-mediated dutp-biotin nick end labelling (tunel) and classical histology (h&e). those follicles showing ≥ 5% tunel positivity (tunel assay) and ... | 2011 | 20070580 |
| in vitro evaluation of liquid-stored buffalo semen at 5°c diluted in soya lecithin based extender (bioxcell®), tris-citric egg yolk, skim milk and egg yolk-citrate extenders. | this study was designed to compare the quality of liquid-stored buffalo bull spermatozoa in soya lecithin based extender bioxcell(®) (biox), milk (milk), tris-citric egg yolk (tey) and egg yolk-citrate (eyc) extender at 5°c. semen was collected from five nili-ravi buffalo (bubalus bubalis) bulls of 6-7 years of age with artificial vagina over a period of 3 weeks (two consecutive ejaculates once in a week). semen ejaculates having more than 60% motility were pooled, split into four aliquots, dilu ... | 2011 | 20070582 |
| genetic and morphometric characterization of a local vietnamese swamp buffalo population. | the water buffalo plays a key role in the socio-economy of south-east asia as it is the main draught power for paddy rice cultivation. while in the indian subcontinent the water buffalo is the riverine type, in south-east asia the majority of buffaloes are of the swamp type. in the poor remote northern province of ha giang in vietnam, improvement of the swamp buffalo breed may be one of the best ways to increase sustainability of farming systems. therefore, analysis of the genetic structure of t ... | 2010 | 20074189 |
| colostrum and serum lysozyme levels in mediterranean buffaloes (bubalus bubalis) and in their newborn calves. | 2010 | 20081181 | |
| effect of timing of development on total cell number and expression profile of hsp-70.1 and glut-1 in buffalo (bubalus bubalis) oocytes and preimplantation embryos produced in vitro. | the present study was designed to compare the expression profile of two developmentally important genes (hsp-70.1 and glut-1) and tcn (total cell number) count in fast (group a) and slow (group b) cleaved buffalo embryos to access their in vitro developmental competence. buffalo cocs (cumulus oocyte complexes) were collected from local abattoir ovaries and subjected to in vitro maturation in: tcm-199 supplemented with 10% fbs (fetal bovine serum), bsa (3 mg/ml), sodium pyruvate (0.25 mm) and 20 ... | 2010 | 20100172 |
| death by attack from a domestic buffalo. | attacks on humans by domestic animals causing fatal injuries are not uncommon in rural areas of india. but injuries due to buffalo gore are rarely observed in villages and are different from other casualties like stab injuries, road fatalities, etc. as the victims of buffalo attack are usually recovered from the fields or forest, the investigating officer could be mislead as to the nature of infliction of fatal injuries to a possible homicide. the injuries caused by the horns of buffaloes are of ... | 2010 | 20129432 |
| sex-preselected buffalo (bubalus bubalis) calves derived from artificial insemination with sexed sperm. | flow cytometry sorting of x- and y-chromosome bearing sperm has been emerging as a promising technology to alter the sex ratio in progenies of mammals in the recent years. the objective of this study was to evaluate the efficiency of ai by using the sexed sperm to produce sex-preselected calves in buffalo species. a total of 43 buffalo cows were inseminated with x-sorted sperm, 30 of which were confirmed pregnant 3 mo following ai. in terms of conception rate, significant difference was observed ... | 2010 | 20137870 |
| morphological changes, dna damage and developmental competence of in vitro matured, vitrified-thawed buffalo (bubalus bubalis) oocytes: a comparative study of two cryoprotectants and two cryodevices. | present study was designed to investigate the impact of two cryodevices; french mini straw (fms) and open pulled straw (ops) using two different cryoprotectants; ethylene glycol (eg) and propylene glycol (pg) on morphological damage, recovery rate, dna damage and developmental competence of in vitro matured vitrified-thawed buffalo oocytes. in vitro matured oocytes were divided into three groups: (a) no cryoprotectant (unfrozen, control), (b) vitrified in fms and (c) in ops using eg/pg. after th ... | 2010 | 20178778 |
| effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (bubalus bubalis) sperm quality following cryopreservation. | cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. under the present study, the cryoprotective effect of taurine and trehalose on buffalo sperm quality parameters after freeze-thaw process was studied. buffalo semen was cryopreserved in tris-based egg yolk extender along with cryoprotectants like taurine (50 mm) or trehalose (100 mm) and used for the assessment of sperm quality parameters like motility, viability, plasma membrane integrity, t ... | 2010 | 20197223 |
| purification and identification of three novel antioxidant peptides from cornu bubali (water buffalo horn). | cornu bubali (water buffalo horn, wbh) has been used in traditional chinese medicine (tcm) for thousands of years. in the present study, three peptides with antioxidant properties were purified from aqueous extract of cornu bubali (water buffalo horn, wbh) by consecutive chromatographic methods including gel filtration chromatography, ion-exchange chromatography and high performance liquid chromatography. the sequences of the three peptides were identified to be gln-tyr-asp-gln-gly-val (wbh-1, 7 ... | 2010 | 20206218 |
| should the domestic buffalo (bubalus bubalis) be considered in the epidemiology of bovine herpesvirus 1 infection? | only limited information is available on the epidemiology and pathogenesis of bovine herpesvirus 1 (bohv-1) in domestic buffalos. in this study, a virulent bohv-1 field strain isolated from cattle was inoculated into buffaloes to evaluate their susceptibility to the virus and to investigate the establishment of viral latency through clinical, virological and serological investigations. latency was also studied by attempting viral reactivation using pharmacological induction. six of seven male, 5 ... | 2010 | 20206452 |
| an indirect elisa for detection of theileria sergenti antibodies in water buffalo using a recombinant major piroplasm surface protein. | in this study we investigated the prevalence and enzootic potential of theileria spp. in water buffalo in the hubei province in china. an indirect elisa based on a recombinant major piroplasma surface protein was developed. the complete orf of the 33-kda major piroplasma surface protein (p33) was obtained from theileria sergenti genomic dna by pcr, cloned into the pet-28(a) vector and expressed in e. coli as a his-fusion protein. then the recombinant p33 (rp33) was purified and used as the antig ... | 2010 | 20207484 |
| multiple molecular forms of inhibin in buffalo (bubalus bubalis) ovarian follicular fluid. | inhibin is a heterodimeric glycoprotein hormone involved in the regulation of fsh release from the anterior pituitary gland and it has been characterized from various animals. although, multiple molecular forms of inhibin have been reported from different species, however, the molecular nature of inhibin has not been studied in buffaloes. in the present study, attempts were made to identify inhibin in buffalo ovarian follicular fluid. buffalo ovaries were obtained from the local abattoir and fol ... | 2010 | 20211478 |
| divergent development of testosterone secretion in male zebu (bos indicus) and crossbred cattle (bos indicus x bos taurus) and buffaloes (bubalus bubalis) during growth. | delayed pubertal development and low fertility of bos indicus x bos taurus crossbred male cattle and domestic buffaloes is hardly understood hence, a sensitive enzymeimmunoassay (eia) was developed using the second antibody-coating technique and testosterone-3-o-carboxymethyloxime-horseradish peroxidase conjugate as a label for determination of testosterone in blood plasma. the eia was validated by standard criteria. blood samples were collected by venipuncture from growing male cattle (karan fr ... | 2010 | 20213223 |
| y-chromosomal variation confirms independent domestications of swamp and river buffalo. | y-chromosomal variation in the water buffalo was analysed by sequencing of dby, zfy and sry gene segments. a clear separation of the paternal lineages of the river and swamp types parallels the differences between their maternal lineages and nuclear dna. sequence divergence was found to be comparable to the divergence of taurine cattle and zebu, and this divergence predated domestication, confirming that river and swamp buffalo originated from different wild populations. within a sample of 23 th ... | 2010 | 20219068 |
| cysteine proteases of trypanosoma (megatrypanum) theileri: cathepsin l-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis. | although trypanosomatheileri and allied trypanosomes are the most widespread trypanosomes in bovids little is known about proteolytic enzymes in these species. we have characterized genes encoding for cathepsin l-like (catl) cysteine proteases from isolates of cattle, water buffalo and deer that largely diverged from homologues of other trypanosome species. analysis of 78 catl catalytic domain sequences from 22 t. theileri trypanosomes disclosed 6 genotypes tightly clustered together into the t. ... | 2010 | 20230907 |
| loop-mediated isothermal amplification (lamp) assay for detection of theileria sergenti infection targeting the p33 gene. | theileria is a widespread, intraerythrocytic tick-borne protozoan parasite. loop-mediated isothermal amplification (lamp) is a novel nucleic acid amplification method offering rapid, accurate and cost-effective diagnosis of infectious diseases. in this study, the lamp method was developed for detecting theileria sergenti. four primers were designed from six distinct regions of the target gene, a 33-kda major piroplasm surface protein (p33) gene in t. sergenti. the specificity assay showed it was ... | 2010 | 20334978 |
| purification, characterization, structural analysis and protein chemistry of a buffalo heart galectin-1. | a soluble β-galactoside-binding lectin was purified by gel filtration chromatography from bubalus bubalis heart. its metal-independent nature, molecular weight of 14.5 kda, preferential affinity for β-d-lactose, and 87-92% identity with carbohydrate recognition domain of previously reported galectin-1 confirmed its inclusion in galectin-1 subfamily. stokes radii determination using gel filtration under reducing and non-reducing conditions revealed its homo-dimeric nature, further confirming its ... | 2010 | 20354738 |
| pineal proteins upregulate specific antioxidant defense systems in the brain. | the neuroendocrine functions of the pineal affect a wide variety of glandular and nervous system processes. beside melatonin (mel), the pineal gland secretes and expresses certain proteins essential for various physiological functions. it has been suggested that the pineal gland may also have an antioxidant role due to secretory product other than mel. therefore, the present study was designed to study the effect of buffalo (bubalus bubalis) pineal proteins (pp) on the antioxidant defense system ... | 2009 | 20357930 |
| zearalenone is bioactivated in the river buffalo (bubalus bubalis): hepatic biotransformation. | zearalenone (zea) as a mycoestrogen is found frequently in human foods and animal feeds. its estrogenic effects depend on its biotransformation fate including both first- and second-phase reactions, which are predominantly governed by hydroxylation and glucuronidation, respectively. in this study, we investigate the hepatic biotransformation of zea in river buffalo. to evaluate the hepatic biotransformation of zea, both subcellular fractions of the liver were prepared. zea was incubated with int ... | 2010 | 20361255 |
| characterization of bison (bison bison) myoglobin. | bison is an alternate meat species gaining increased popularity in north america. although previous investigations reported that bison meat discolors faster than beef, the molecular basis of this observation has not been investigated. therefore, the objective of the present study was to determine the redox stability, thermostability, and primary structure of bison myoglobin (mb), in comparison with beef mb. purified bison and beef myoglobins were analyzed for autoxidation, lipid oxidation-induce ... | 2010 | 20374756 |
| changes in luteal cells distribution, apoptotic rate, lipid peroxidation levels and antioxidant enzyme activities in buffalo (bubalus bubalis) corpus luteum. | buffalo (bubalus bubalis) is known for its weak/silent estrous behaviour, lower conception rate and longer inter-calving interval as compared to cattle. understanding the kinetics and functional properties of luteal cells may be helpful to improve reproductive efficiency in the buffalo. hence the present study was designed to assess the size and distribution of steroidogenic luteal cells along with biochemical properties during different phases of corpus luteum (cl) in the buffalo. the ovaries c ... | 2010 | 20378285 |
| genetic parameters for stayability in murrah buffaloes. | in order to contribute to the breeding programmes of asian water buffalo, the aim of this study was to analyse the influence of genetic effects in the stayability of murrah dairy buffaloes. the stayability trait (st) was defined as the female's ability to stay in the herd for one (st1), two (st2), three (st3), four (st4), five (st5) or six years (st6) after the first calving. the same trait was also considered as continuous and was designated stayability in days up to one (std1), two (std2), thr ... | 2010 | 20380776 |
| histology of hemal nodes of the water buffalo (bos bubalus). | hemal nodes are independent lymphoid organs found in various mammals but are ignored by most immunologists. they seem to play a role in defense against blood-borne infections in some species. the structure of the hemal node has been described in various species but, so far, not in the water buffalo. specimens were obtained from ten clinically healthy male animals (five calves: 2-3 months old; five bulls: 2-8 years old). six hemal nodes were obtained from each animal from the mesenteric and perir ... | 2010 | 20387076 |
| antipyretic and antioxidant activities of the aqueous extract of cornu bubali (water buffalo horn). | cornu bubali (water buffalo horn, wbh) is an animal-derived product which is widely used in traditional chinese medicine (tcm) for dispelling heat, relieving convulsions and cooling blood. the purpose of this study was to investigate the antipyretic activity of wbh aqueous extract and its potential mechanism. two hyperthermia models, yeast-induced (infectious) and skimmed milk-induced (noninfectious) hyperthermia were employed to evaluate the antipyretic effect and the results showed that rectal ... | 2010 | 20387226 |
| combining aboriginal and non-aboriginal knowledge to assess and manage feral water buffalo impacts on perennial freshwater springs of the aboriginal-owned arnhem plateau, australia. | aboriginal land managers have observed that feral asian water buffalo (bubalis bubalis lydekker) are threatening the ecological and cultural integrity of perennial freshwater sources in arnhem land, australia. here we present collaborative research between the aboriginal rangers from warddeken land management limited and western scientists which quantified the ground-level impacts of buffalo on seven perennial freshwater springs of the arnhem plateau. a secondary aim was to build the capacity of ... | 2010 | 20390401 |
| efficacy of brucella abortus vaccine strain rb51 compared to the reference vaccine brucella abortus strain 19 in water buffalo. | approximately 250,000 water buffalo (bubalus bubalis) live in the campania region of southern italy where the breeding of this species is very popular. of these animals, almost 150,000 are concentrated in the caserta province where the prevalence of brucella abortus in this species represents approximately 20% at herd level. the italian brucellosis eradication programme provides a slaughter and vaccination strategy for this province. b. abortus strain rb51 (rb51) has become the official vaccine ... | 2010 | 20391363 |
| effect of source and dose of probiotics and exogenous fibrolytic enzymes (efe) on intake, feed efficiency, and growth of male buffalo (bubalus bubalis) calves. | probiotics of lactobacillus acidophilus, saccharomyces cerevisiae, and aspergillus niger and three commercial exogenous fibrolytic enzymes (efe) were tested in vitro to select best source and optimum dose followed by in vivo studies on male buffalo calves. bacterial (p < 0.001) and protozoal population were increased significantly (p < 0.001) with probiotics and efe. in vitro dry matter digestibility was more (p < 0.001) on l. acidophilus and then on s. cerevisiae. dose required for l. acidophil ... | 2010 | 20401692 |
| quantification, morphology and ultrastructure of preantral follicles of buffalo (bubalus bubalis) foetuses. | the objective of this study was to determine the number, morphology and ultrastructure of preantral ovarian follicles of buffalo (bubalus bubalis) foetuses at different ages. quantification revealed number of primordial, primary and secondary follicles of 48,857 ± 17,506, 26,000 ± 20,452, 18,428 ± 10,875 and 18,375 ± 19,690, 225 ± 349, 326 ± 288 at 12-34 cm and 35-60 cm crown rump length (crl), respectively. follicular diameter values were 28.9 (± 3.4), 34.7 (± 5.9) and 59.4 (± 12.6) μm; oocyte ... | 2011 | 20403125 |
| localization and expression of follicle-stimulating hormone receptor gene in buffalo (bubalus bubalis) pre-antral follicles. | follicle-stimulating hormone (fsh) stimulates antral follicles to grow, but its role in earlier stages (pre-antral) of follicle development, if any, is obscure. aim of this study was to study the expression of follicle-stimulating hormone receptor (fshr) gene in different sizes of pre-antral follicles (pfs) (<150, 200, 250, 300, 350, 400 μm) and to find out an optimum dose of fsh for better growth, development and steroidogenesis of pfs in vitro. buffalo ovaries were collected from a local abatt ... | 2011 | 20403128 |
| a serological survey of crimean-congo haemorrhagic fever in animals in the sharkia governorate of egypt. | a surveillance survey was conducted over a period of 12 months between september 2004 and august 2005 by the tropical medicine department of zagazig university in collaboration with naval medical research unit no. 3 (namru-3), egypt, to evaluate the role of ruminants as a reservoir host for crimean-congo haemorrhagic fever virus (cchfv). a total of 1,022 serum samples from 313 cattle, 264 water buffalo (bubalus bubalis), 270 sheep and 175 goats were included in the survey. all samples were colle ... | 2008 | 20405447 |
| progesterone supplementation during multiple ovulation treatment in buffalo species (bubalus bubalis). | the aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. fourteen mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 iu of fsh and lh starting on day 8 of the cycle. in group a (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group b (n = 7) it was left till day 10, when ... | 2010 | 20411328 |
| risk factors associated with the presence of positive reactions in the sccit test in water buffalo around two cities in punjab, pakistan. | the present study was undertaken to find out the association of certain risk factors with the positive sccit (single comparative cervical intradermal tuberculin) test in water buffaloes at faisalabad and okara, pakistan. seventy-six herds (697 buffaloes) at faisalabad and 56 (395 buffaloes) at okara were included in the study. the buffaloes were screened through sccit test. positive reaction to sccit test was recorded in 14% of herds and in 3% of buffaloes. the herds positive for this test were ... | 2010 | 20412784 |
| hot topic: gene duplication at the α-lactalbumin locus: finding the evidence in water buffalo (bubalus bubalus l.). | studies on milk proteins revealed that a qualitative and quantitative polymorphism may often be found regarding alpha-lactalbumin (alpha-la). in mammals, a similar phenomenon was widely documented in the alpha-globin system as the result of a gene duplication. the presence of several differently expressed alpha-lactalbumin gene (lalba) products suggests that the mechanism underlying this phenomenon may involve nonallelic genes. to check this hypothesis, an experiment was set up to investigate th ... | 2010 | 20412931 |
| bluetongue virus in south america: overview of viruses, vectors, surveillance and unique features. | since the first published report of bluetongue (bt) virus (btv) infection in south america from brazil in 1978, serological surveys have determined that the infection is widespread in sheep, cattle, goats and water buffalo but generally without clinical signs. only four outbreaks of bt disease have been reported so far in brazil. brazil and argentina are the only countries in south america where btv serotypes 12 and 4 have been isolated, respectively. by serology, serotypes 4, 6, 14, 17, 19 and ... | 2004 | 20419641 |
| bluetongue in italy: part ii. | in summer 2000, bluetongue (bt) infection was reported in italy and caused a widespread epidemic involving a total of ten southern and central regions and is still in progress after three years. from the date of the first case (18 august 2000) to 14 may 2001, when the lowpoint in the first epidemic curve was reached, a total of 310,234 animals in 6,869 flocks of three regions had been involved. from 15 may 2001 to 14 april 2002, when a second epidemic wave swept through central and southern ital ... | 2004 | 20419673 |
| bluetongue vaccination in europe: the italian experience. | the incursion of bluetongue (bt) into italy in august 2000 caused heavy economic losses, partly due to the disease itself, but mostly because of disruption caused to the national animal trade structure. to limit direct losses and the circulation of bt virus (btv), the italian ministry of health ordered, on 11 may 2001, the vaccination of all susceptible domestic ruminant species (i.e. sheep, goats, cattle and water buffalo) in both infected and surrounding areas. the vaccination strategy was bas ... | 2004 | 20422595 |
| effects of vaccination against bluetongue on milk production and quality in cattle vaccinated with live-attenuated monovalent type 2 vaccine. | the first epidemic of bluetongue (bt) to affect the three regions of sardinia, sicily and calabria (italy) in 2000 induced high economic losses caused by the disease itself and by the cessation of ruminant movements both within, and out of, the infected areas. in order to reduce virus circulation, and to create a resistant livestock population, the italian ministry of health ruled, in may 2001, that all sheep, cattle, goats and water buffalo, in infected and in neighbouring regions, be vaccinate ... | 2004 | 20422604 |
| cellular coupling of potassium channels with beta2 adrenoceptors in mediating myometrial relaxation in buffaloes (bubalus bubalis). | present study unravels the functional presence of potassium channels and their involvement in mediating beta(2) adrenoceptors-induced myometrial relaxation in buffalo myometrium. isolated myometrial preparations exhibited rhythmic spontaneity with regular pattern of amplitude and frequency. levcromakalim produced concentration-dependent inhibitory effect on myometrial spontaneity and relaxant effect and the dose-response curve (drc) was shifted towards right in the presence of glybenclamaide. in ... | 2010 | 20444021 |
| genomic characterization and chromosomal mapping of 5 river buffalo skeletal muscle differentiation master genes. | river buffalo (bubalus bubalis, 2n = 50, bbu) is a species of economic relevance in a number of countries. this species shows a very peculiar biology and a great capacity for environmental adaptation. there has been an increasing economic interest as well as a growing demand for a more detailed knowledge of molecular features in this species. from this perspective we report a genomic, transcriptional and cytogenetic analysis of 5 master genes involved in skeletal muscle development. of these 5 g ... | 2010 | 20453476 |
| genome-wide search of the genes tagged with the consensus of 33.6 repeat loci in buffalo bubalus bubalis employing minisatellite-associated sequence amplification. | minisatellites have been implicated with chromatin organization and gene regulation, but mrna transcripts tagged with these elements have not been systematically characterized. the aim of the present study was to gain an insight into the transcribing genes associated with consensus of 33.6 repeat loci across the tissues in water buffalo, bubalus bubalis. using cdna from spermatozoa and eight different somatic tissues and an oligo primer based on two units of consensus of 33.6 repeat loci (5' cct ... | 2010 | 20480223 |
| characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (bubalus bubalis) calves. | recently, a coronavirus strain (179/07-11) was isolated from water buffalo (bubalus bubalis) and the virus which displayed a strict genetic and biological relatedness with bovine coronavirus (bcov) was referred to as bubaline coronavirus (bucov). here, we report the characterisation of four bucovs strains identified in the faeces or intestinal contents of water buffalo calves with acute gastroenteritis. single bucov infections were detected in all but one cases from which two clostridia species ... | 2010 | 20483547 |
| toward milk speciation through the monitoring of casein proteotypic peptides. | the possibility of detecting extraneous milk in singles species cheese-milk has been explored. a mass spectrometry (ms)-based procedure has been developed to detect 'signature peptides', corresponding to the predefined subset of 'proteotypic peptides', as matchless analytical surrogates of the parent caseins. tryptic digests of skimmed milk samples from four species were analyzed by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms. amongst the candidate signature peptide ... | 2010 | 20486267 |
| short communication: identification and technological characterization of yeast strains isolated from samples of water buffalo mozzarella cheese. | sixty yeast cultures were isolated from samples of water buffalo mozzarella, a popular "pasta filata" cheese, originating on 16 farms located in the provinces of salerno, caserta, and frosinone (italy). strains were identified by means of 5.8s internal transcribed spacer rdna pcr-rflp combined with 26s rrna gene partial sequencing and characterized for their ability to exert biochemical properties of technological interest. the recorded dominance of fermenting yeasts such as the lactose-fermenti ... | 2010 | 20494142 |
| genetic diversity in farm animals--a review. | domestication of livestock species and a long history of migrations, selection and adaptation have created an enormous variety of breeds. conservation of these genetic resources relies on demographic characterization, recording of production environments and effective data management. in addition, molecular genetic studies allow a comparison of genetic diversity within and across breeds and a reconstruction of the history of breeds and ancestral populations. this has been summarized for cattle, ... | 2010 | 20500753 |
| effect of pineal proteins at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in rats. | pineal glands secrets melatonin and various proteins and peptides which has many physiological functions. in keeping with this view, present experiment was conducted to know the effect of buffalo (bubalus bubalis) pineal proteins (pp) at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in female rats. for this, we took 30 adult female wistar rats (133-145 g body weights, bw) and divided into five groups (control, group i; 150 ppm fluoride (f) ... | 2011 | 20509005 |
| prevalence, biology, and distribution pattern of sarcocystis infection in water buffalo (bubalus bubalis) in iran. | this study was conducted to determine the prevalence, distribution pattern, and the sarcocystis species involved in slaughtered water buffaloes (bubalus bubalis) in the khuzestan, iran by macroscopic and histological examination. the esophagus, heart, diaphragm, tongue, masseter, and thigh muscles were investigated. esophagus and thigh muscles of only 3 of the 100 examined water buffaloes (3%) were infected with macroscopic sarcocystis, whereas at microscopic level sarcocystis were found in 83 o ... | 2010 | 20524065 |
| effects of chemical activation treatment on development of swamp buffalo (bubalus bubalis) oocytes matured in vitro and fertilized by intracytoplasmic sperm injection. | the objective of this study was to optimize the activation protocol for buffalo oocytes after intracytoplasmic sperm injection (icsi). the release of the second polar body (pb) at 3, 6 and 9 h after icsi of in-vitro matured oocytes activated either with 5 μm ionomycin (io) or with 7% ethanol (etoh) was preliminary examined. the highest rate of second pb extrusion occurred at 3 h of activation, and the second pb extrusion in etoh group was significantly higher than that in io group. oocytes that ... | 2011 | 20546174 |
| follicular dynamics and oestrous detection in thai postpartum swamp buffaloes (bubalus bubalis). | this study characterized follicular activity and oestrous behaviour from 5 to 9 days post-calving up to the 4th ovulation postpartum (pp) in 16 multiparous (range 2-7 parities) thai swamp buffalo cows (bubalus bubalis), aged 4-12 years and weighing from 432 to 676 kg. ovarian follicular activity was examined by transrectal ultrasonography (tus) every morning. oestrous detection was performed twice daily by direct personal observation of behaviour and for presence of clear cervical mucus discharg ... | 2011 | 20546176 |
| cryopreservation of buffalo (bubalus bubalis) semen in bioxcell extender. | this study was designed to compare commercially available extender bioxcell with tris-citric egg yolk extender for post thaw quality and in vivo fertility of buffalo semen. for comparison of post thaw semen quality: semen was collected from five adult nili-ravi buffalo (bubalus bubalis) bulls of similar age group with artificial vagina (at 42 degrees c) for three weeks (replicates). qualifying ejaculates having motility >60% from each buffalo bull were divided in two aliquots and diluted (at 37 ... | 2010 | 20570331 |
| ultrastructure of in vitro oocyte maturation in buffalo (bubalus bubalis). | the objective of the present study was to describe ultrastructural changes in the nucleus and cytoplasmic organelles during in vitro maturation (ivm) of buffalo cumulus-oocyte complexes (cocs). the structures were collected by ovum pick-up (opu). some cocs, removed from maturation medium at 0, 6, 12, 18 and 24 h, were processed for transmission electron microscopy. the average number of cocs collected by opu/animal/session was 6.4, and 44% of them were viable. immature oocytes had a peripherally ... | 2010 | 20576206 |
| water buffalo genome science comes of age. | the water buffalo is vital to the lives of small farmers and to the economy of many countries worldwide. not only are they draught animals, but they are also a source of meat, horns, skin and particularly the rich and precious milk that may be converted to creams, butter, yogurt and many cheeses. genome analysis of water buffalo has advanced significantly in recent years. this review focuses on currently available genome resources in water buffalo in terms of cytogenetic characterization, whole ... | 2010 | 20582226 |
| ketosis in buffalo (bubalus bubalis): clinical findings and the associated oxidative stress level. | as little is known about the oxidant/antioxidant status in buffalo with ketosis, the present study was delineated to assess the oxidative stress level associated with clinical ketosis in water buffalo. a total of 91 parturient buffalo at smallholder farms were studied (61 suspected to be ketotic and 30 healthy). clinical and biochemical investigations were carried out for each buffalo. based on clinical findings and the level of beta-hydroxybutyrate (bhb), buffalo were allocated into ketotic (42 ... | 2010 | 20582718 |
| beneficial impact on cardiovascular risk profile of water buffalo meat consumption. | meat is a good source of proteins and irons, yet its consumption has been associated with unfavorable cardiovascular effects. whether this applies to all types of meat is unclear. we thus aimed to appraise the impact of water buffalo meat consumption on cardiovascular risk profile with an observational longitudinal study. | 2010 | 20588291 |
| the role of mc1r gene in buffalo coat color. | melanocortin-1 receptor (mc1r) plays a major role in pigmentation in many species. to investigate if the mc1r gene is associated with coat color in water buffalo, the coding region of mc1r gene of 216 buffalo samples was sequenced, which included 49 black river buffalo (murrah and nili-ravi), 136 swamp buffalo (dehong, diandongnan, dechang, guizhou, and xilin) with white and gray body, and 31 hybrid offspring of river buffalo nili-ravi (or murrah) and swamp buffalo. among the three variation sit ... | 2010 | 20596837 |
| comparative efficacy of immunological, molecular and culture assays for detection of group a rotavirus from faecal samples of buffalo (bubalus bubalis) calves. | group a rotaviruses play an important role in causing gastroenteritis and mortality in buffalo (bubalus bubalis) calves. a number of assays like rna-polyacrylamide gel electrophoresis (rna-page), enzyme-linked immunosorbent assay (elisa), reverse transcription polymerase chain reaction (rt-pcr) and virus isolation have been employed for rotavirus diagnosis. we evaluated the comparative efficacy of different assays for detection of group a rotavirus in buffalo calves. a total of 455 faecal sample ... | 2010 | 20607400 |
| comparative study on responses of cattle and water buffalo (bubalus bubalis) to experimental inoculation of brucella abortus biovar 1 by the intraconjunctival route--a preliminary report. | the preliminary study was conducted to assess the virulence of a strain of brucella abortus (1969d) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. seven of each cattle and water buffalo (bubalus bubalis) calves aged 3-6 months were inoculated intraconjunctivally with counts ranging from 1.5 × 10(7) to 1.7 × 10(10) colony forming units of b. abortus. animals were monitored over an 8-week period for clinical manifestations and sero ... | 2010 | 20614238 |
| the influence of cumulus cells during in vitro fertilization of buffalo (bubalus bubalis) denuded oocytes that have undergone vitrification. | the aim of this work was to evaluate whether providing a support of cumulus cells during ivf of buffalo denuded oocytes submitted to vitrification-warming enhances their fertilizing ability. in vitro matured denuded oocytes were vitrified by cryotop in 20% eg + 20% of dmso and 0.5 m sucrose and warmed into decreasing concentrations of sucrose (1.25 m-0.3m). oocytes that survived vitrification were fertilized: 1) in the absence of a somatic support (dos); 2) in the presence of bovine cumulus cell ... | 2010 | 20615538 |
| study on the inter-subspecies nuclear transfer of river buffalo somatic cell nuclei into swamp buffalo oocyte cytoplasm. | the objective of this study was to explore the feasibility of inter-subspecies somatic cell nuclear transfer (scnt) of river buffalo (50 chromosomes) somatic cell nuclei into swamp buffalo (48 chromosomes) oocyte cytoplasm. the enucleated swamp buffalo oocytes were fused with four different types of river buffalo cells: freshly thawed ear fibroblasts, serum-starved ear fibroblasts, cumulus cells and ear fibroblasts from a cloned buffalo calf. as a result, the developmental competence of embryos ... | 2010 | 20621244 |
| impact of livestock hygiene education programs on mastitis in smallholder water buffalo (bubalus bubalis) in chitwan, nepal. | a project implemented from 2003 to 2005 trained women in chitwan district, nepal, in hygienic dairy production using a process of social mobilization. the aim of this research was to assess if the prevalence of mastitis in water buffalo in the households of women who were trained was lower one year after training than in untrained households, if the training influenced knowledge and practices for the prevention or control of mastitis, and if these practices and knowledge were associated with a l ... | 2010 | 20655119 |
| prevalence and molecular diagnosis of trypanosoma evansi in nili-ravi buffalo (bubalus bubalis) in different districts of punjab (pakistan). | the prevalence of trypanosoma evansi was investigated in 1,250 nili-ravi buffaloes of mixed age and sex by polymerase chain reaction (pcr) for the first time in pakistan. dna of the trypanosomes was isolated with trireagent®. the assay was employed using primers esag 6/7, specific for a 237-bp fragment from t. evansi genomic dna. the samples were screened for the presence of t. evansi also by stained thin smear. forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were ... | 2010 | 20680446 |
| tissue-specific promoter methylation coincides with cyp19 gene expression in buffalo (bubalus bubalis) placenta of different stages of gestation. | aromatase is the key enzyme for estrogen biosynthesis and is encoded by cyp19 gene. placental cotyledons are the main site of cyp19 gene expression during pregnancy. the present study was aimed to investigate if dna methylation and thus epigenetic mechanisms play a potential role in stage-specific regulation of cyp19 expression in placental cotyledons of pregnant water buffaloes (bubalus bubalis). significantly higher expression of cyp19 gene (p<0.05) in placental cotyledons of early gestation p ... | 2010 | 20691690 |
| sequence analysis and genetic variability of stearoyl coa desaturase (scd) gene in the italian mediterranean river buffalo. | stearoyl-coa desaturase (scd) plays a key metabolic role by changing the saturated fa content of ruminant milk and meat. in this study we characterized for the first time the stearoyl-coa desaturase (scd) gene in river buffalo (bubalus bubalis) and investigated its genetic variability. on a total of 78 buffaloes, 15 snps were detected and 6 of them were preliminarily genotyped. in particular, the g.133a>c snp was found to create a new consensus site for the sp1 binding site, thus generating a ne ... | 2010 | 20691778 |
| a rare case of centric fission and fusion in a river buffalo (bubalus bubalis, 2n = 50) cow with reduced fertility. | a 5-year-old river buffalo cow underwent cytogenetic investigation since it had only one male offspring, apparently with normal body constitution, which died one month after birth. the female carrier had normal body conformation and internal sex adducts, as revealed by rectal palpation performed by a specialist veterinary practitioner. the cow was found to carry a complex and rare chromosome abnormality. indeed, a centric fission of one river buffalo (bbu) chromosome 1 with a subsequent (or simu ... | 2011 | 20693780 |
| comparative potential of cultured skin fibroblast, cumulus, and granulosa cell to produce somatic cell nuclear transfer (scnt) preimplantation embryos in buffaloes (bubalus bubalis) in relation to gene expressions. | to improve the efficiency of somatic cell nuclear transfer (scnt)-derived embryos in buffaloes (bubalus bubalis), skin fibroblast, cumulus, and granulosa cells were cultured up to the 15th passage and cloned embryos were produced from each cell type. at the 15th passage the cumulative population doublings (cpds) in cumulus cells was higher (60.78) than skin fibroblast (57.12) and granulosa (56.05) cell lines. gene expression of chromatin remodelling proteins, that is, hdac1, dnmt1, dnmt3a, and d ... | 2010 | 20698775 |
| spermatogonia-specific proteins expressed in prepubertal buffalo (bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia. | buffalo is an economically important livestock species in asia. little is known about male germ line technology owing to lack of sufficient understanding regarding expression of germ- and somatic-cell specific-proteins in the testis. in this study, we identified uchl-1 (pgp 9.5) and lectin- dolichos biflorus agglutinin (dba) as specific markers for spermatogonia in buffalo testis. expression of germ-cell and pluripotency-specific proteins such as ddx4 (vasa) and pou5f1 (oct3/4) were also present ... | 2010 | 20708247 |
| radionuclide concentration ratios in australian terrestrial wildlife and livestock: data compilation and analysis. | radionuclide concentrations in australian terrestrial fauna, including indigenous kangaroos and lizards, as well as introduced sheep and water buffalo, are of interest when considering doses to human receptors and doses to the biota itself. here, concentration ratio (cr) values for a variety of endemic and introduced australian animals with a focus on wildlife and livestock inhabiting open rangeland are derived and reported. the cr values are based on u- and th-series concentration data obtained ... | 2010 | 20711841 |
| bacterial diversity in the rumen of indian surti buffalo (bubalus bubalis), assessed by 16s rdna analysis. | bacterial communities in buffalo rumen were characterized using a culture-independent approach for a pooled sample of rumen fluid from 3 adult surti buffaloes. buffalo rumen is likely to include species of various bacterial phyla, so 16s rdna sequences were amplified and cloned from the sample. a total of 191 clones were sequenced and similarities to known 16s rdna sequences were examined. about 62.82% sequences (120 clones) had >90% similarity to the 16s rdna database sequences. furthermore, ab ... | 2010 | 20720314 |
| identification, characterization and expression analysis of hepcidin gene in sheep. | hepcidin is part of the innate immune system, and it plays a central role in the regulation of iron homeostasis. this peptide has been previously characterized in man, non-human primates, rat, mouse, dog, swine, cattle, horse, fishes, reptiles and birds but until now not in sheep. the aim of this study was to sequence, characterize and perform hepcidin expression analysis in different tissues collected from healthy sheep. the resulting open reading frame consisted of 249bp predicted to encode an ... | 2011 | 20723953 |
| allelic diversity at mhc class ii dq loci in buffalo (bubalus bubalis): evidence for duplication. | the genetic diversity of mhc class ii dq genes was investigated in riverine buffalo (bubalus bubalis) by pcr-rflp and sequencing. highly variable regions (exons 2-3) of dq genes were amplified from 152 buffaloes and genotyped by pcr-rflp. alleles identified by differential restriction patterns were sequenced for the characterization. pcr-rflp was a rapid method to discriminate between dqa1 and duplicated dqa2 genes in buffalo, however, the method appeared to be inadequate for determining the mor ... | 2010 | 20724005 |
| peptides from water buffalo cheese whey induced senescence cell death via ceramide secretion in human colon adenocarcinoma cell line. | milk proteins are a source of bioactive peptides. recent studies have indicated that protein-derived peptides released in buffalo cheese acid whey exert a cytomodulatory effect in human epithelial colon cancer (caco2) cells. the aim of the present study was to explain the molecular mechanism involved in the response of caco2 cells to oxidative stress in the presence of peptide fractions of buffalo cheese whey, purified and characterized by mass spectrometry. | 2011 | 20725925 |
| growth, metabolic status and ovarian function in buffalo (bubalus bubalis) heifers fed a low energy or high energy diet. | the aim was to establish the capacity of buffalo heifers to adapt their metabolic requirements to a low energy diet. murrah buffalo (bubalus bubalis) heifers undergoing regular estrous cycles were randomly assigned by age, live weight (lw) and body condition score (bcs) to a high energy group (he, 5.8 milk forage units (mfu)/day, n=6) or low energy group (le, 3.6 mfu/day, n=6). circulating concentrations of metabolic substrates, metabolic hormones and reproductive hormones were determined weekly ... | 2010 | 20729015 |
| molecular characterization and expression profile of uterine serpin (serpina14) during different reproductive phases in water buffalo (bubalus bubalis). | uterine serpins (serpina14) play important roles during pregnancy in the farm animals. in this study, we have cloned and characterized cdna sequence encoding the bubaline serpina14. we also studied its spatio-temporal expression in the uterine endometrium. the bubaline serpina14 has an open reading frame of 1299bp. itshares ∼90% identity with the serpina14 of other ruminant livestock species. phylogenetically, bubaline serpina14 has been placed in the same clade that contained other mammalian ho ... | 2010 | 20801587 |
| stenotrophomonas maltophilia as a causal agent of pyogranulomatous hepatitis in a buffalo (bubalus bubalis). | a 7-year-old female buffalo (bubalus bubalis) from a local herd in serres, northern greece, was presented to a private veterinary clinic with a chronic loss of appetite for 15 days. the clinical examination revealed high fever (41.5 degrees c), lethargy, yellow discoloration of skin and mucous membranes, an abdomen that appeared to be empty, hyperactive rumen motility, and tachypnea. a biochemical profile revealed an elevated total bilirubin concentration and hepatic enzyme activities, whereas g ... | 2010 | 20807941 |
| early pregnancy detection of iraqi riverine buffalo (bubalus bubalis) using the biopryn enzyme-linked immunosorbent assay for pspb and the progesterone assay. | this study was undertaken to detect pregnancy in iraqi riverine buffalo (bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy-specific protein b (pspb) with the biopryn(®) enzyme-linked immunosorbent assay (elisa) test. the aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. twenty-two female riverine buffalo that were 6.0 ± 0.93 years old were used. four ... | 2011 | 20825581 |
| activation of zona-free buffalo (bubalus bubalis) oocytes by chemical or electrical stimulation, and subsequent parthenogenetic embryo development. | parthenogenetic activation using zona-free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. the aim of this study was to compare the efficacy of different methods for the activation of zona-free buffalo oocytes in terms of their effects on the developmental competence of parthenogenetic embryos. the effects of zona removal on parthenogenetic activation and in vitro developmental competence of metaphase ii oocy ... | 2011 | 20825585 |
| the microanatomy of the palatine tonsils of the buffalo (bos bubalus). | the palatine tonsils play a key role in initiating immune responses against antigens entering the body through the mouth. they are also replication sites of some pathogens. there is no data available about the structure of the palatine tonsils of the egyptian water buffalo. therefore, palatine tonsils of 14 clinically healthy buffalo bulls (2-3 years old) were examined macroscopically and microscopically using light, and transmission electron microscopes. the tonsils had an elongated kidney shap ... | 2011 | 20833436 |
| freeze-thaw induced genotoxicity in buffalo (bubalus bubalis) spermatozoa in relation to total antioxidant status. | use of cryopreserved semen has become an important tool in assisted reproduction but freezing and thawing cause sub-lethal damage to spermatozoa. this is detrimental to sperm because of the membrane damage including permeability and integrity. an excess generation of reactive oxygen species (ros) creates oxidative stress due to reduced antioxidant status of the cryopreserved spermatozoa. in the present study fresh buffalo semen was collected and divided into two aliquots. one aliquot was used fo ... | 2011 | 20853151 |
| reproductive cycles of buffalo. | the domestic water buffalo (bubalus bubalis) has an important role in the agricultural economy of many developing countries in asia, providing milk, meat and draught power. it is also used in some mediterranean and latin american countries as a source of milk and meat for specialized markets. although the buffalo can adapt to harsh environments and live poor quality forage, reproductive efficiency is often compromised by such conditions, resulting in late sexual maturity, long postpartum anoestr ... | 2010 | 20869822 |
| immunolocalization of pulmonary intravascular macrophages, tlr4, tlr9 and il-8 in normal and pasteurella multocida-infected lungs of water buffalo (bubalus bubalis). | water buffalo are of considerable economic significance in south east asia, but these animals suffer from many bacterial respiratory diseases including haemorrhagic septicaemia caused by pasteurella multocida. bacterial respiratory diseases of animals cause lung inflammation that is characterized by the activation of toll-like receptors (tlrs) expressed on macrophages, expression of chemokines and recruitment of neutrophils and monocytes. pulmonary intravascular macrophages (pims) present in the ... | 2010 | 20880542 |
| dynamic in vivo changes in the activities of gelatinases, matrix metalloproteinases (mmps), and tissue inhibitor of metalloproteinases (timps) in buffalo (bubalus bubalis) uterine luminal fluid during estrous cycle and early pregnancy. | in ruminants, the phenomenon of endometrial tissue remodeling during the estrous cycle and early pregnancy is not fully understood. in this report, the occurrence of tissue remodeling, if any, in buffalo endometrium was studied by detecting gelatinases, matrix metalloproteinases (mmps) and tissue inhibitors of metalloproteinases (timps); the key regulators of tissue remodeling, in uterine luminal fluids (ulf) of cycling and early pregnant (approx. 43-65 days) buffaloes. each stage of the estrous ... | 2010 | 20886603 |
| eating and rumination behaviour in brahman grade cattle and crossbred water buffalo fed on high roughage diet. | the purpose of this study is to compare feeding and other behavior and nutrient digestibility of tropical grade brahman (body weight (bw) = 231 kg ± 12.4; n = 3) and crossbred water buffalo (bw = 300 kg ± 13.9; n = 3). this experiment on digestibility and measures of muscles of mastication utilized one-way, and animal behavior two-way, analysis of variance, respectively. two video camcorders were installed in each pair of buffalo and brahman for 24 h period programmed on the 107th, 109th and 111 ... | 2010 | 20887310 |
| seroprevalence of toxoplasma gondii in water buffaloes (bubalus bubalis) in south-west of iran. | the prevalence of antibodies to toxoplasma gondii was conducted in 300 buffaloes from ahvaz, kouzestan province, southwest of iran. blood sera were screened using a modified agglutination test (mat) incorporating 2-mercaptoethanol. positive reactions in sera dilutions above 1:25 were considered as indicative for the presence of t. gondii antibodies. the overall prevalence of infection in the animals was 14.33% with titers of 1:25 in 21, 1:50 in 12, 1:100 in 6, 1:200 in 2 and 1:400 in 2. the prev ... | 2010 | 20962725 |
| sarcocystis cruzi: comparative studies confirm natural infections of buffaloes. | controversy exists concerning whether cattle and water buffalo sustain infections with cysts of distinct arrays of species in the genus sarcocystis. in particular, morphologically similar parasites have been alternately ascribed to sarcocystis cruzi or to sarcocystis levinei, depending on their occurrence in cattle or water buffalo. we used light and transmission electron microscopy, genetic analysis, and experimental infections of definitive canine hosts to determine whether consistent differen ... | 2010 | 20971107 |
| virulence of brucella abortus isolated from cattle and water buffalo. | brucellosis has been documented in domestic water buffalo (bubalus bubalis) but published literature is limited despite the importance of this species in tropical agricultural systems. the objective of this study was to compare the virulence of brucella abortus isolates recovered from cattle and water buffalo. nineteen strains of b. abortus from cattle and domestic water buffalo in trinidad were intraperitoneally inoculated into balb/c mice. spleens were cultured for b. abortus and histopatholog ... | 2010 | 20972706 |
| vaccine to save water buffalo for field work rushed to china. | 1946 | 20995910 | |
| bilateral follicular cysts in a water buffalo. | the present short communication puts on record a case of bilateral, multiple follicular cysts in a water buffalo along with a detailed description of its ovarian biometry and follicular fluid composition. the ovarian weight and biometrical parameters were much higher than in normal cycling buffaloes. a total of three follicular cysts were observed, two on the right ovary and one on the left ovary, measuring 4.9, 3.0 and 2.6 cm yielding 21, 9 and 5 ml of follicular fluid, respectively. the cystic ... | 2010 | 21086160 |
| experimental infection of dogs (canis familiaris) with sporulated oocysts of neospora caninum. | neospora caninum is widely distributed in the world and this parasite is one of the major causes of abortion in cattle. dogs and coyotes are definitive hosts of n. caninum and several species of domestic and wild animals are intermediate hosts. dogs can become infected by the ingestion of tissues containing cysts and then excrete oocysts. it is not yet known whether sporulated oocysts are able to induce a patent infection in dogs, i.e. a shedding of n. caninum oocysts in feces. the objective of ... | 2010 | 21094584 |
| temporal changes in circulating levels of plasma interleukin-8 during peripartum period in murrah buffaloes (bubalus bubalis) under subtropical climate. | the objective of this study was to elucidate the changes in circulating levels of plasma interleukin-8 (il-8) during peripartum period in murrah buffaloes (bubalus bubalis). il-8 was estimated in blood plasma of healthy peripartum murrah buffaloes (n=6) on days ± 30, ± 15, ± 5, ± 3, ± 1 and 0 pre- and postpartum with respect to the day of parturition (day 0) in each of the two different seasons (hot-humid and spring). the mean microclimate temperature-humidity index (thi) during spring season wa ... | 2010 | 21107908 |
| selection of possible marker peptides for the detection of major ruminant milk proteins in food by liquid chromatography-tandem mass spectrometry. | the aim of this work was the determination of peptides, which can function as markers for identification of milk allergens in food samples. emphasis was placed on two casein proteins (α- and β-casein) and two whey proteins (α-lactalbumin and β-lactoglobulin). in silico tryptic digestion provided preliminary information about the expected peptides. after tryptic digestion of four milk allergens, the analytical data obtained by combination of reversed-phase high performance liquid chromatography a ... | 2010 | 21107975 |
| purification and biological characterization of bacterially expressed recombinant buffalo prolactin. | recombinant prolactin (prl) from water buffalo (bubalus bubalis) has been cloned and expressed in a prokaryotic expression system. the hormone was also successfully refolded into a biologically active form. total rna was purified from buffalo pituitaries and the buprl cdna was synthesized using primers designed on bovine prl sequence. this prolactin cdna was cloned in a pet 28a vector and expressed in escherichia coli strain bl21(de3)plyss. most of the expressed protein was present as insoluble ... | 2010 | 21108131 |
| sequence of specific mitochondrial 16s rrna gene fragment from egyptian buffalo is used as a pattern for discrimination between river buffaloes, cattle, sheep and goats. | characterization of molecular markers and the development of better assays for precise and rapid detection of domestic species are always in demand. this is particularly due to recent food scares and the crisis of biodiversity resulting from the huge ongoing illegal traffic of endangered species. the aim of this study was to develop a new and easy method for domestic species identification (river buffalo, cattle, sheep and goat) based on the analysis of a specific mitochondrial nucleotide sequen ... | 2010 | 21116860 |
| buffalo hepcidin: characterization of cdna and study of antimicrobial property. | hepcidin, a novel gene encoded, 25 residue, 2-3 kda cysteine rich cationic peptide synthesized in liver cells play an important role in iron metabolism in addition to its antimicrobial activity. in this study hepcidin cdna expressed on hepatocytes of bubalus bubalis has been characterized and the antibacterial activity of buffalo hepcidin analog has been determined. the complete buffalo hepcidin sequence is of 324 bp and have an orf of 249 nucleotides from 14 to 262. the sequence analysis at nuc ... | 2010 | 21161377 |
| the effect of season on oocyte quality and developmental competence in italian mediterranean buffaloes (bubalus bubalis). | at italian latitudes, buffalo (bubalus bubalis) is a seasonally polyestrous species, showing an improved reproductive efficiency when daylight decreases (autumn). the aim of the present study was to evaluate the influence of the season on buffalo oocyte recovery rate, on oocyte quality, assessed on morphological basis, and developmental competence after in vitro fertilization. for this purpose, buffalo ovaries were collected from a local abattoir and the oocytes obtained by aspirating the follic ... | 2010 | 21168984 |
| [genetic characteristics on exon 4 of prolactin gene in 12 water buffalo populations]. | the prolactin exerts obvious adjustment and control function for mammary gland development, lactation and milk protein gene expression in water buffalo. in this study the sequence features and polymorphisms of the exon 4 in prolactin gene were examined in 385 individuals which came from 12 river and swamp type buffalo populations using dna direct sequencing and pcr-sscp methods. the results showed that the sequence of exon 4 in prolactin gene was consists of 180 nucleotides, the fragment had hig ... | 2010 | 21174345 |
| identification of capacitation associated tyrosine phosphoproteins in buffalo (bubalus bubalis) and cattle spermatozoa. | at ejaculation mammalian sperm lack fertilizing ability as they are released in a functionally immature form. the capacity to fertilize eggs is only acquired after they have been educated in the female reproductive tract and this phenomenon is termed as capacitation. sperm capacitation includes a cascade of biochemical modifications, including cholesterol efflux, ca(2+) influx and camp/pka-dependent/independent protein tyrosine phosphorylation which is specifically considered as the biochemical ... | 2010 | 21177049 |
| evidence of congenital transmission of neospora caninum in naturally infected water buffalo (bubalus bubalis) fetus from brazil. | the aim of this study was to determine the congenital infection by neospora caninum in the water buffalo (bubalus bubalis), a natural intermediate host. nine pregnant water buffalos, raised under free-grazing condition, were slaughtered, and their fetuses were collected. samples of brain and thoracic fluid were obtained from those fetuses, with gestational ages ranging from 2 to 5 months. the dna of n. caninum was detected and identified in the brain of one of those fetuses, using two pcr assays ... | 2010 | 21181191 |