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production of oligosaccharide from alginate using pseudoalteromonas agarovorans.a marine bacterium was isolated from seawater near the korean south coast for efficient saccharification from alginate. based on 16s rdna sequence, the isolated strain was identified as pseudoalteromonas agarovorans. various environmental factors affecting saccharification of alginate using p. agarovorans cho-12 have been investigated in flask cultures. the optimum concentration of sugar was obtained at 30 rpm and 29 degrees c. among various nacl concentrations, when nacl concentration was incre ...200919160071
tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies.tigecycline has been investigated in combination with other antibacterials against a wide range of susceptible and multiresistant gram-positive and gram-negative bacteria. combinations have been analysed in vitro, in animal models and in human case reports. in vitro, tigecycline combined with other antimicrobials produces primarily an indifferent response (neither synergy nor antagonism). nevertheless, synergy occurred when tigecycline was combined with rifampicin against 64-100% of enterococcus ...200919162449
changes in bacterial isolates from burn wounds and their antibiograms: a 20-year study (1986-2005).our aim is to elucidate shifts in the bacterial spectrum colonising burn wounds and corresponding antibiotic susceptibilities during a 20-year study period.200919167827
stenotrophomonas maltophilia pacemaker endocarditis in a patient with d-transposition of the great arteries after atrial switch procedure.we report a case of pacemaker endocarditis due to stenotrophomonas maltophilia in a 22-year-old caucasian man with d-transposition of the great arteries after atrial switch procedure. s.maltophilia isolated from blood cultures was susceptible to trimethoprim-sulfamethoxazole and amikacin, and resistant to ciprofloxacin and all tested β-lactam antibiotics. the infected pacemaker system was completely removed by thoracotomy. simultaneously, a new ddd pacemaker and epicardial electrodes were succes ...201019171390
prevalence of infectious pathogens in crohn's disease.the importance of infectious pathogens in crohn's disease (cd) is still under debate. therefore, we examined a panel of potential viral and bacterial pathogens in a large series of cd patients and controls. archival tissue from 76 patients, 56 with cd and 20 control patients, with normal colon mucosa (n=10) and non-steroid anti-inflammatory drug (nsaid)-induced colitis (n=10) were examined using pcr-based detection methods for human cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex ...200919186006
[application of biological aerated filter in treating biomass wastewater and its microbial population characteristics].biomass wastewater contains plenty of cellulose, hemicellulose, starch, saccharide, organic acid and protein. it has high cod value and leads to environment pollution and resource waste. biomass wastewater was treated in this investigate using a biological aerated filter (baf) on laboratory scale, and the effect of treatment in different hydraulic retention time (hrt) had been studied. the results show that the optimum hrt is 10 h. the value of cod and tn in effluent is 74.90 mg/l and 1.21 mg/l ...200819186816
investigation of bacterial pathogens on 70 frequently used environmental surfaces in a large urban u.s. university.after reports of increased severity of bacterial infections from community institutions, a broad spectrum of 70 surfaces was sampled for potential bacterial pathogens in the morning and afternoon of one day per week over three consecutive weeks in a large u.s. university. surfaces included public telephone mouthpieces, water fountain drains, student computer keyboards and desks, and buttons on elevators, vending machines, and photocopiers. a total of 420 samples was obtained. bacterial counts we ...200919192740
current status of newer carbapenems.beta-lactam antibiotics represent the most commonly prescribed antibacterial agents. since many bacteria have developed resistance to older agents, new beta-lactams have been introduced constantly. in the late 1970s, a new class of exceptionally broad-spectrum beta-lactams, the carbapenems, was identified. the carbapenems, such as the most popular imipenem and meropenem, have the widest spectra of antibacterial activity of all the beta-lactams and provide excellent coverage of many gram-negative ...200919199922
characterization of the charge variants of l2 beta-lactamase in stenotrophomonas maltophilia.stenotrophomonas maltophilia kh has two acid beta-lactamases with isoelectric points (pis) of 4.6 and 5.4, and several basic beta-lactamases (pis >7.0) that produce a ladder-shaped pattern by ief. an isogenic l2 mutant, khl2(xyle), was constructed by gene replacement. from ief and native page zymograms of strains kh and khl2(xyle), it was demonstrated that the basic beta-lactamases and the acid beta-lactamase with pi 5.4 are encoded by the same l2 gene and that the active types of these l2 charg ...200919208880
stenotrophomonas chelatiphaga sp. nov., a new aerobic edta-degrading bacterium.a new ethylenediaminetetraacetic acid (edta)-utilizing gammaproteobacterial strain lpm-5(t) was isolated from municipal sewage sludge. aerobic, gram-negative, motile rods multiply by binary fission. neutrophilic and mesophilic, these are unable to grow in the presence of 3% nacl (w/v), and unable to reduce nitrate to nitrite, and are oxidase and catalase positive, but lipase negative. the major cellular fatty acids are c(i15:0), c(a15:0) and c(16:1w7c). the dominant phospholipids are phosphatidy ...200919216044
community-acquired stenotrophomonas maltophilia infections: a systematic review.stenotrophomonas maltophilia is a pathogen that causes infections mainly in immunocompromised patients. however, community-acquired s. maltophilia infections have been occasionally reported. the objective of this paper was to collect and evaluate the available published data referring to community-acquired s. maltophilia infections. we searched pubmed, the cochrane library, and scopus for articles providing data for patients with community-acquired s. maltophilia infections. eight case series an ...200919224257
structure and mechanism of copper- and nickel-substituted analogues of metallo-beta-lactamase l1.in an effort to further probe metal binding to metallo-beta-lactamase l1 (mbetal l1), cu- (cu-l1) and ni-substituted (ni-l1) l1 were prepared and characterized by kinetic and spectroscopic studies. cu-l1 bound 1.7 equiv of cu and small amounts of zn(ii) and fe. the epr spectrum of cu-l1 exhibited two overlapping, axial signals, indicative of type 2 sites with distinct affinities for cu(ii). both signals indicated multiple nitrogen ligands. despite the expected proximity of the cu(ii) ions, howev ...200919228020
species distribution and antimicrobial susceptibility of gram-negative aerobic bacteria in hospitalized cancer patients.nosocomial infections pose significant threats to hospitalized patients, especially the immunocompromised ones, such as cancer patients.200919228413
antimicrobial activity of three tick defensins and four mammalian cathelicidin-derived synthetic peptides against lyme disease spirochetes and bacteria isolated from the midgut.in this study, chemically synthesized tick defensins and cathelicidin-derived mammalian peptides were used to investigate the activity spectrum against borrelia garinii and symbiotic stenotrophomonas maltophila. synthetic tick defensins showed antimicrobial activity against staphylococcus aureus but not b. garinii and s. maltophila. mammalian peptides which have cationic property similar to tick defensins, showed antimicrobial activity similar to tick defensins. the antimicrobial peptides in tic ...200919229642
[microbiological diagnosis of bronchopulmonary colonization-infection in cystic fibrosis].cystic fibrosis (cf), a condition produced by mutations in the gene that encodes the cystic fibrosis transmembrane conductance regulator, is the most prevalent autosomal-recessive hereditary disease in caucasian populations. among other repercussions, this defect leads to an alteration of respiratory secretions and determines a predisposition for chronic bronchopulmonary colonization-infection, which is the main driver of the high morbidity and early mortality of cf patients. colonization by sta ...200919232782
pneumonia in solid organ recipients: spectrum of pathogens in 217 episodes.lower respiratory tract infection (lrti) remains a leading cause of morbidity and mortality after solid organ transplantation (sot).200919249559
bacterial contamination of cystic fibrosis clinics.respiratory pathogens from cf patients can contaminate inpatient settings, which may be associated with increased risk of patient-to-patient transmission. few data are available that assess the rate of bacterial contamination of outpatient settings. we determined the frequency of contamination of cf clinics and the effectiveness of alcohol-based disinfectants in reducing hand carriage of bacterial pathogens.200919250885
a stenotrophomonas maltophilia multilocus sequence typing scheme for inferring population structure.stenotrophomonas maltophilia is an opportunistic, highly resistant, and ubiquitous pathogen. strains have been assigned to genogroups using amplified fragment length polymorphism. hence, isolates of environmental and clinical origin predominate in different groups. a multilocus sequence typing (mlst) scheme was developed using a highly diverse selection of 70 strains of various ecological origins from seven countries on all continents including strains of the 10 previously defined genogroups. se ...200919251858
low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients.pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ...200919261796
tylenchulus semipenetrans alters the microbial community in the citrus rhizosphere.infection of citrus seedlings by tylenchulus semipenetrans was shown to reduce subsequent infection of roots by phytophthora nicotianae and to increase plant growth compared to plants infected by only the fungus. hypothetical mechanisms by which the nematode suppresses fungal development include nutrient competition, direct antibiosis, or alteration of the microbial community in the rhizosphere to favor microorganisms antagonistic to p. nicotianae. a test of the last hypothesis was conducted via ...200319265991
development of an autofluorescent organophosphates-degrading stenotrophomonas sp. with dehalogenase activity for the biodegradation of hexachlorocyclohexane (hch).simultaneous biodegradation of hexachlorocyclohexane (hch) and organophosphates (ops) by a recombinant stenotrophomonas sp. was studied in the study. the broad-host-range plasmid pvgab, harboring enhanced green fluorescent protein gene (egfp) and dehalogenase genes (lina and linb), was constructed and transformed into the op-degrading strain stenotrophomonas yc-1 to get the recombinant strain yc-h. over-expression of dehalogenase (lina and linb) and enhanced green fluorescent protein (egfp) was ...200919269809
clinical investigation of isolated bacteria from urinary tracts of hospitalized patients and their susceptibilities to antibiotics.complicated urinary tract infections (utis) are often difficult to treat, partly because of the existence of isolated antibiotic-resistant strains. even though the definition of uti is determined by the quantity of cultured bacteria, it has been unclear if the quantity of cultured uti bacteria affects their susceptibility to antimicrobial agents. also, the gram stain is generally performed to assume the causative bacteria and their quantity before culture results can be obtained; therefore, we c ...200919280295
morphological evidence of biofilm formation in greenlanders with chronic suppurative otitis media.biofilm may explain the recurrences and recalcitrant episodes of otorrhea in chronic suppurative otitis media (csom). this study investigates bacterial biofilm in greenlanders with csom and chronic otitis media with effusion (come). the study is partly blinded, prospective and retrospective. six children with csom, four children with come and ten adults with csom were included in this study. cultures were obtained and examined by standard methods. otorrhea or glue was collected from the children ...200919283404
incidence and risk factors of early bacterial infections after unrelated cord blood transplantation.incidence and characteristics of early bacterial infection within 100 days after unrelated cord blood transplantation (ucbt) were assessed for 664 pediatric and 1208 adult recipients in japan. cumulative incidence of early bacterial infection at day 100 post-ucbt was 11% (95% confidence interval [ci], 8%-13%) for children and 21% (ci, 19%-24%) for adults (p < .0001). early bacterial infection in adults had a significant impact on mortality (hazard ratio [hr] = 2.1, ci, 1.7-2.6; p < .0001), altho ...200919285631
factors associated with bacteremia due to multidrug-resistant gram-negative bacilli in hematopoietic stem cell transplant recipients.the epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of gram-negative (gn) bacteria and the development of multidrug resistance (mdr) among gn bacteria. we conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant gram-negative (mdrgn) isolates in hematopoietic stem cell transplant recipients. ten patients with mdrgn bacteremia were compared with 44 patients with gn bacteremia wi ...200919287908
structural and functional analysis of smet, the repressor of the stenotrophomonas maltophilia multidrug efflux pump smedef.stenotrophomonas maltophilia is an opportunistic pathogen characterized for its intrinsic low susceptibility to several antibiotics. part of this low susceptibility relies on the expression of chromosomally encoded multidrug efflux pumps, with smedef being the most relevant antibiotic resistance efflux pump so far studied in this bacterial species. expression of smedef is down-regulated by the smet repressor, encoded upstream smedef, in its complementary dna strand. in the present article we pre ...200919324881
aflatoxin b(1) degradation by stenotrophomonas maltophilia and other microbes selected using coumarin medium.aflatoxin b(1) (afb(1)) is one of the most harmful mycotoxins in animal production and food industry. a safe, effective and environmentally sound detoxification method is needed for controlling this toxin. in this study, 65 samples were screened from various sources with vast microbial populations using a newly developed medium containing coumarin as the sole carbon source. twenty five single-colony bacterial isolates showing afb(1) reduction activity in a liquid culture medium were selected fro ...200819325817
evaluation of the vitek 2 cards for identification and antimicrobial susceptibility testing of non-glucose-fermenting gram-negative bacilli.we evaluated vitek 2 cards (ngnc and ast-gn10) for the accuracy of identification (id) and antimicrobial susceptibility testing (ast) of non-glucose-fermenting gram-negative bacilli (ngf-gnb). in a total of 201 strains, 190 strains (94.5%) were correctly identified, seven strains (3.5%)showed low discrimination, four strains (2.0%) had discrepancies, and no strain remained unidentified.reference ast of amikacin, aztreonam, cefepime, cefotaxime, ceftazidime, ciprofloxacin, imipenem,levofloxacin, ...200919343822
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
activity of levofloxacin and ciprofloxacin on biofilms and planktonic cells of stenotrophomonas maltophilia isolates from patients with device-associated infections.the aim of this work was to compare the in vitro effects of levofloxacin and ciprofloxacin on pre-formed biofilms and planktonic populations of stenotrophomonas maltophilia isolates from device-associated infections recovered over the period 2004-2008 at a university hospital in argentina. for planktonic susceptibility studies, the minimum inhibitory concentrations (mics) of fluoroquinolones were determined by the broth microdilution method. from the 32 isolates recovered, 84.4% were sensitive t ...200919362452
early- and late-onset pneumonia: is this still a useful classification?the choice of empirical treatment of nosocomial pneumonia in the intensive-care unit (icu) used to rely on the interval after the start of mechanical ventilation. nowadays, however, the question of whether in fact there is a difference in the distribution of causative pathogens is under debate. data from 308 icus from the german national nosocomial infection surveillance system, including information on relevant pathogens isolated in 11,285 cases of nosocomial pneumonia from 1997 to 2004, were u ...200919364852
[results of 4 years of microbiological testing of bronchoscopes].in the context of reducing endoscopy-related infectious risk and new national guidelines on microbiological samples from bronchoscopy, the results of a surveillance program set up in a hospital center were analyzed.200919367202
survey of gut colonisation with stenotrophomonas maltophilia among neonates. 200919380180
pseudomonas aeruginosa las quorum sensing autoinducer suppresses growth and biofilm production in legionella species.bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (qs). recent studies have shown that the las qs autoinducer n-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-c(12)-hsl) of pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. in this study, we report the effects of pseudomonas 3-oxo-c(12)-hsl on the growth and suppression of virulence factors ...200919383702
identification of lysine positive non-fermenting gram negative bacilli (stenotrophomonas maltophilia and burkholderia cepacia complex).the burkholderia cepacia complex (bcc) and stenotrophomonas maltophilia are closely related groups of non-fermenting gram-negative bacilli (nfgnbs) having a similar spectrum of infections ranging from superficial to deep-seated and disseminated infections. identification of these lysine decarboxylase-positive nfgnbs lags behind in most indian laboratories. a simplified identification scheme was devised for these two pathogens that allowed us to isolate them with an increasing frequency at our te ...200919384035
stenotrophomonas maltophilia: an emerging opportunist human pathogen.stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in the debilitated host. s maltophilia is not an inherently virulent pathogen, but its ability to colonise respiratory-tract epithelial cells and surfaces of medical devices makes it a ready coloniser of hospitalised patients. s maltophilia can cause blood-stream infections and pneumonia with considerable morbidity in immunosuppressed patients. management of infection is hampered by high-level intrinsic resistance to ...200919393961
volatile organic compounds: a potential direct long-distance mechanism for antagonistic action of fusarium oxysporum strain msa 35.fusarium oxysporum msa35 [wild-type (wt) strain] is an antagonistic fusarium that lives in association with a consortium of bacteria belonging to the genera serratia, achromobacter, bacillus and stenotrophomonas in an italian soil suppressive to fusarium wilt. typing experiments and virulence tests provided evidence that the f. oxysporum isolate when cured of the bacterial symbionts [the cured (cu) form], is pathogenic, causing wilt symptoms identical to those caused by f. oxysporum f. sp. lactu ...200919396945
[comparison between agar dilution and three other methods for determining the susceptibility of 228 clinical isolates of non-fermenting gram-negative rods].susceptibility testing for non-fermenting gram-negative rods (nfgnr) is problematic; valid methods are needed for this purpose.200919406523
analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by dgge.a microbial consortium was obtained by enrichment culture of sea water samples collected from botan oil port in xiamen, china, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. denaturing gradient gel electrophoresis (dgge) was used to investigate the bacterial composition and community dynamic changes based on pcr amplification of 16s rrna genes during batch culture enrichment. using the spray-plate method, three bacteria, designated a ...200919409577
ampdi is involved in expression of the chromosomal l1 and l2 beta-lactamases of stenotrophomonas maltophilia.two ampd homologues, ampd(i) and ampd(ii), of stenotrophomonas maltophilia have been cloned and analyzed. comparative genomic analysis revealed that the genomic context of the ampd(ii) genes is quite different, whereas that of the ampd(i) genes is more conserved in s. maltophilia strains. the ampd system of s. maltophilia is distinct from that of the enterobacteriaceae and pseudomonas aeruginosa in three respects. (i) ampd(i) of s. maltophilia is not encoded in an ampde operon, in contrast to wh ...200919414581
hydroxylation of thiacloprid by bacterium stenotrophomonas maltophilia cgmcc1.1788.chloropyridinyl neonicotinoid insecticides play a major role in crop protection and flea control on cats and dogs. imidacloprid, thiacloprid and acetamiprid have in common the 6-chloro-3-pyridinylmethyl group but differ in the nitroguanidine or cyanoamidine substituent on an acyclic or cyclic moiety. our previous study found that stenotrophomonas maltophilia cgmcc 1.1788 could hydroxylate imidacloprid to 5-hydroxy imidacloprid, and 5-hydroxy imidacloprid was easily converted to 10-19 times highe ...200919421875
bacterial contamination of patients' medical charts in a surgical ward and the intensive care unit: impact on nosocomial infections.the purpose of this study was to determine the degree of bacterial contamination of patients' files, and to compare the colonized bacteria between files from the surgical intensive care unit (icu) and the surgical ward at the wan fang hospital, taipei, taiwan.200919424563
stenotrophomonas maltophilia endogenous endophthalmitis: clinical presentation, sensitivity spectrum and management.stenotrophomonas maltophilia is gaining importance as a community-acquired pathogen, after becoming firmly established as a nosocomial pathogen. here we report a case of endogenous endophthalmitis due to s. maltophilia. antibiotic-susceptibility testing of the isolate was performed by the kirby-bauer disc diffusion method. the organism was resistant to aminoglycosides, imipenem, ticarcillin and cotrimoxazole and was sensitive to ceftazidime and chloramphenicol. the patient was successfully treat ...200919429764
control of pseudomonas aeruginosa and stenotrophomonas maltophilia contamination of microfiltered water dispensers with peracetic acid and hydrogen peroxide.the abilities of peracetic acid and hydrogen peroxide to remove or reduce pseudomonas aeruginosa and stenotrophomonas maltophilia in output water from microfiltered water dispensers (mwds) were investigated. two mwds were inoculated with strains of p. aeruginosa and s. maltophilia isolated from water. dispensers a and b were disinfected with 10% (v/v) peracetic acid (paa) and 3% (v/v) hydrogen peroxide (hp) respectively. each dispenser was disinfected three times at monthly intervals with contac ...200919439386
cationic compounds with activity against multidrug-resistant bacteria: interest of a new compound compared with two older antiseptics, hexamidine and chlorhexidine.use of antiseptics and disinfectants is essential in infection control practices in hospital and other healthcare settings. in this study, the in vitro activity of a new promising compound, para-guanidinoethylcalix[4]arene (cx1), has been evaluated in comparison with hexamidine (hx) and chlorhexidine (chx), two older cationic antiseptics. the mics for 69 clinical isolates comprising methicillin-resistant staphylococcus aureus, methicillin-sensitive s. aureus, coagulase-negative staphylococci (co ...201019456831
[antimicrobial resistance of clinical isolates of stenotrophomonas maltophilia].to investigate the antimicrobial resistance of clinical isolates of stenotrophomonas matophilia (sma) and the mechanisms of their drug resistance.200919460691
diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients: guidelines of the infectious diseases working party of the german society of haematology and oncology.patients with neutropenia lasting for more than 10d, who develop fever and pulmonary infiltrates, are at risk of treatment failure under conventional broad-spectrum antibacterial therapy. filamentous fungi are predominant causes of failure, however, multi-resistant gram-negative rods such as pseudomonas aeruginosa or stenotrophomonas maltophilia may be involved. prompt addition of mould-active systemic antifungal therapy, facilitated by early thoracic computed tomography, improves clinical outco ...200919467584
isolation and genetic identification of pah degrading bacteria from a microbial consortium.polycyclic aromatic hydrocarbons (pah; naphthalene, anthracene and phenanthrene) degrading microbial consortium c2pl05 was obtained from a sandy soil chronically exposed to petroleum products, collected from a petrochemical complex in puertollano (ciudad real, spain). the consortium c2pl05 was highly efficient degrading completely naphthalene, phenanthrene and anthracene in around 18 days of cultivation. the toxicity (microtox method) generated by the pah and by the intermediate metabolites was ...200919468841
design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.the aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of sechium edule fluid extract and to obtain a pharmaceutical formulation with them. the extract exhibited antimicrobial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, serratia marcescens, morganella morganii, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia, candida spp. and aspergillus spp. isolated fr ...200919477252
extremophile culture collection from andean lakes: extreme pristine environments that host a wide diversity of microorganisms with tolerance to uv radiation.a total of 88 bacterial strains were isolated from six andean lakes situated at altitudes ranging from 3,400 to 4,600 m above sea level: l. aparejos (4,200 m), l. negra (4,400 m), l. verde (4,460 m), l. azul (4,400 m), l. vilama (4,600 m), and salina grande (3,400 m). salinity ranged from 0.4 to 117 ppm. general diversity was determined by denaturing gradient gel electrophoresis (dgge) analysis. from the excised dgge bands, 182 bacterial sequences of good quality were obtained. gammaproteobacter ...200919495855
respiratory microbiology of patients with cystic fibrosis in the united states, 1995 to 2005.numerous improvements in diagnostic and therapeutic strategies for patients with cystic fibrosis (cf) have occurred during the past 2 decades. we hypothesized that these changes could impact trends in respiratory microbiology.200919505987
efficacy of bacterial consortium-aie2 for contemporaneous cr(vi) and azo dye bioremediation in batch and continuous bioreactor systems, monitoring steady-state bacterial dynamics using qpcr assays.bacterial consortium-aie2 with a capability of contemporaneous cr(vi) reduction and azo dye rv5 decolourization was developed from industrial wastewaters by enrichment culture technique. the 16s rrna gene based molecular analyses revealed that the consortium bacterial community structure consisted of four bacterial strains namely, alcaligenes sp. dma, bacillus sp. dmb, stenotrophomonas sp. dms and enterococcus sp. dme. cumulative mechanism of cr(vi) reduction by the consortium was determined usi ...200919517254
in vitro bactericidal activity of the n-terminal fragment of the frog peptide esculentin-1b (esc 1-18) in combination with conventional antibiotics against stenotrophomonas maltophilia.in this study the bactericidal effect of the n-terminal fragment of the frog skin peptide esculentin-1b [esc(1-18)] in combination with clinically used antimicrobial agents was evaluated against stenotrophomonas maltophilia, either in standard conditions (phosphate buffer) or in the presence of human serum. a synergistic bactericidal effect was observed after a 24h incubation when combinations of esc(1-18) and amikacin or colistin were used against clinical strains of s. maltophilia with or with ...200919520127
prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamase-producing bacteria in intensive care units of sanandaj general hospitals (kurdistan, iran).this study focused on analyzing the spread of extended-spectrum beta-lactamase (esbl) enzymes among gram-negative bacteria at intensive care units (icus). between january 2007 and january 2008, 301 consecutive clinical isolates of gram-negative type were isolated. of these, 66 strains were collected from patients in icus in two major hospitals in sanandaj (kurdistan, iran). the isolates were identified, tested for antimicrobial susceptibility, and analyzed for the presence of esbl using the doub ...200919521074
clinical importance of stenotrophomonas maltophilia nosocomial pneumonia due to its high mortality in hemodialysis patients.patients undergoing hemodialysis are immunocompromised and can suffer from pneumonia with various pathogens in nosocomial conditions. we investigated the fundamental information on the characteristics of hemodialysis inpatients and nosocomial pneumonia. we surveyed 1803 hemodialysis patients admitted to our university hospital between 2001 and 2007. the mean patient age was 64.8 years and the average period of hospitalization was 28.1 days, which was considerably longer than the average stay in ...200919527465
the versatility and adaptation of bacteria from the genus stenotrophomonas.the genus stenotrophomonas comprises at least eight species. these bacteria are found throughout the environment, particularly in close association with plants. strains of the most predominant species, stenotrophomonas maltophilia, have an extraordinary range of activities that include beneficial effects for plant growth and health, the breakdown of natural and man-made pollutants that are central to bioremediation and phytoremediation strategies and the production of biomolecules of economic va ...200919528958
enhanced degradation of tnt by genome-shuffled stenotrophomonas maltophilia ok-5.in this study, the enhanced degradation of tnt using cultures of genome-shuffled stenotrophomonas maltophilia ok-5 mt-3 has been examined and the proteome of shuffled strain was compared to the wild-type ok-5 strain. genome shuffling of s. maltophilia ok-5 was used to achieve a rapid enhancement of tnt degradation. the initial mutant population was generated by ntg treatment and uv irradiation. the wild-type ok-5 strain was able to degrade 0.2 mm tnt within 6 days, yet barely tolerated 0.5 mm tn ...200919543944
metabolism of acenaphthylene via 1,2-dihydroxynaphthalene and catechol by stenotrophomonas sp. rmsk.stenotrophomonas sp. rmsk capable of degrading acenaphthylene as a sole source of carbon and energy was isolated from coal sample. metabolites produced were analyzed and characterized by tlc, hplc and mass spectrometry. identification of naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid, 1,2-dihydroxynaphthalene, salicylate and detection of key enzymes namely 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase in the cell free extract suggest that a ...200919543983
simultaneous degradation of organophosphates and 4-substituted phenols by stenotrophomonas species lz-1 with surface-displayed organophosphorus hydrolase.organophosphorous hydrolase (oph) was expressed onto the surface of a stenotrophomonas species (lz-1), capable of simultaneously degrading 4-substituted phenols, using the n- and c-terminal domains of ice nucleation protein (inpnc) as an anchoring motif for the first time. the engineered strain lz-1 could degrade p-nitrophenyl-substituted organophosphates as well as their hydrolytic product, pnp, rapidly. especially, addition of 4-cp (below 0.8 mm) significantly accelerated the complete degradat ...200919548671
stenotrophomonas maltophilia isolated from the airways of animals with chronic respiratory disease.stenotrophomonas maltophilia (s. maltophilia) is a nonfermentative bacterium, which is naturally resistant against a panel of commonly-used antibiotics. it is frequently isolated from humans with chronic respiratory disease, e.g. cystic fibrosis or chronic obstructive pulmonary disease. in veterinary medicine s. maltophilia is perceived to be a mere coloniser. we herewith report 7 strains of s. maltophilia isolated from animals, of which 5 strains were harvested from 3 horses, a dog and a cat wi ...200919565454
susceptibility of 228 non-fermenting gram-negative rods to tigecycline and six other antimicrobial drugs.the aim of the study was to determine the in vitro activity of tigecycline and 6 other antimicrobial drugs used in clinical practice against 228 clinical isolates of nonfermenting gram-negative rods (nfgnrs) including acinetobacter spp., stenotrophomonas maltophilia, and pseudomonas aeruginosa. minimum inhibitory concentrations (mics) were determined according to the recommendations of the clinical and laboratory standards institute. for tigecycline, we used the criteria approved by the fda. alm ...200919567346
successful treatment of stenotrophomonas maltophilia soft tissue infection with tigecycline: a case report. 200919567363
plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture.plant-associated microorganisms fulfill important functions for plant growth and health. direct plant growth promotion by microbes is based on improved nutrient acquisition and hormonal stimulation. diverse mechanisms are involved in the suppression of plant pathogens, which is often indirectly connected with plant growth. whereas members of the bacterial genera azospirillum and rhizobium are well-studied examples for plant growth promotion, bacillus, pseudomonas, serratia, stenotrophomonas, and ...200919568745
broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis.broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples.200919570210
stenotrophomonas maltophilia genomes: a start-up comparison.the whole dna sequences of 2 stenotrophomonas maltophilia strains isolated from the blood of a cancer patient (k279a) and the poplar populus trichocarpa (r551-3) have been compared. the 2 chromosomes exhibit extensive synteny, but each is punctuated by about 40 genomic islands (geis), which vary in size from 3 to 70kb, and may encode up to about 50 proteins. a large set of smaller dna sequences, encoding strain-specific 'solo' orfs, contributes to genetic heterogeneity in a significant manner. s ...200919574092
cough-generated aerosols of pseudomonas aeruginosa and other gram-negative bacteria from patients with cystic fibrosis.pseudomonas aeruginosa is the most common bacterial pathogen in patients with cystic fibrosis (cf). current infection control guidelines aim to prevent transmission via contact and respiratory droplet routes and do not consider the possibility of airborne transmission. it was hypothesised that subjects with cf produce viable respirable bacterial aerosols with coughing.200919574243
molecular characterization of stenotrophomonas maltophilia isolates from cystic fibrosis patients and the hospital environment.to ascertain whether cystic fibrosis (cf) patients are colonized or infected with unique or multiple strains of stenotrophomonas maltophilia; to understand whether some strains colonize or infect more than 1 patient, indicating clonal spread; and to explore the molecular heterogeneity of hospital water isolates and their correlation with clinical isolates.200919583515
bsac standardized disc susceptibility testing method (version 8).there have been considerable changes to the format of the recommendations since the previous version (version 7). the majority of the footnotes to the tables have been removed and the notations added to the end column; it is hoped that this change will avoid confusion in interpretation. antibiotics have been separated into groups, e.g. beta-lactams, aminoglycosides, etc. recommendations for urinary tract infections (utis) have been removed for most agents except for those that are administered s ...200919587067
association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in taiwan.this study was designed to determine the strength of the association between the isolation of nonfermentative gram-negative bacilli (nfgnb) from tap water faucet aerators and the prevalence of colonization or infection of patients in intensive care units (icus). surveillance cultures were obtained during a 4-month period from 162 faucet aerators located in seven different icus. the prevalence of colonization or infection of icu patients with nfgnb was determined by prospective surveillance durin ...200919587299
risk factors for relapse of ventilator-associated pneumonia in trauma patients.our goal was to define risk factors for ventilator-associated pneumonia (vap) relapse and examine the implications, if any, for initial therapy in trauma patients.200919590315
sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos.to evaluate the efficacy of ultraviolet (uv) irradiation for rapid microbial decontamination of liquid nitrogen (ln2).201019591992
dicamba monooxygenase: structural insights into a dynamic rieske oxygenase that catalyzes an exocyclic monooxygenation.dicamba (2-methoxy-3,6-dichlorobenzoic acid) o-demethylase (dmo) is the terminal rieske oxygenase of a three-component system that includes a ferredoxin and a reductase. it catalyzes the nadh-dependent oxidative demethylation of the broad leaf herbicide dicamba. dmo represents the first crystal structure of a rieske non-heme iron oxygenase that performs an exocyclic monooxygenation, incorporating o(2) into a side-chain moiety and not a ring system. the structure reveals a 3-fold symmetric trimer ...200919616009
crystal structure of dicamba monooxygenase: a rieske nonheme oxygenase that catalyzes oxidative demethylation.dicamba (3,6-dichloro-2-methoxybenzoic acid) is a widely used herbicide that is efficiently degraded by soil microbes. these microbes use a novel rieske nonheme oxygenase, dicamba monooxygenase (dmo), to catalyze the oxidative demethylation of dicamba to 3,6-dichlorosalicylic acid (dcsa) and formaldehyde. we have determined the crystal structures of dmo in the free state, bound to its substrate dicamba, and bound to the product dcsa at 2.10-1.75 a resolution. the structures show that the dmo act ...200919616011
a novel bacterial isolate stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticles.abstract:200919619318
tigecycline: a new treatment option for intra-abdominal infections.tigecycline is the first of a new class of antibiotics, the glycylcyclines, with properties which can overcome many common resistance mechanisms, making it appropriate for treatment of many serious and life-threatening infections for which other antibiotics are no longer appropriate. its wide antibacterial spectrum includes most methicillin-resistant staphylococci, vancomycin-resistant enterococci, esbl-producing and other multi-resistant gram-negative bacteria such as acinetobacter and stenotro ...200919622449
multidrug resistant gram-negative bacilli as predominant bacteremic pathogens in liver transplant recipients.bacteremias, which are often caused by gram-negative bacteria, are the most frequently occurring infectious complications after liver transplantation (lt). the aim of this study was to investigate bacteremic incidence, pathogenic spectrum, risk factors for bacteremia due to multidrug resistant (mdr) gram-negative bacilli, and its impact on mortality after lt.200919638006
bioinspired synthesis of hierarchical macro-mesoporous titania with tunable macroporous morphology using cell-assemblies as macrotemplates.cell-assemblies with different cell shapes were used as macrotemplates in the bioinspired synthesis of hierarchical macro-mesoporous titania with tunable macroporous morphology and enhanced photocatalytic performance.200919641831
molecular cloning and characterization of smra, a novel abc multidrug efflux pump from stenotrophomonas maltophilia.stenotrophomonas maltophilia is an emerging nosocomial pathogen that can cause difficult-to-treat infections and exhibits significant degrees of poorly understood multidrug resistance (mdr). the aim of this study was to identify and characterize a multidrug atp-binding cassette (abc) efflux pump in s. maltophilia.200919643774
motion of the zinc ions in catalysis by a dizinc metallo-beta-lactamase.we report rapid-freeze-quench x-ray absorption spectroscopy of a dizinc metallo-beta-lactamase (mbetal) reaction intermediate. the zn(ii) ions in the dinuclear active site of the s. maltophilia class b3 mbetal move away from each other, by approximately 0.3 a after 10 ms of reaction with nitrocefin, from 3.4 to 3.7 a. together with our previous characterization of the resting enzyme and its nitrocefin product complex, where the zn(ii) ion separation relaxes to 3.6 a, these data indicate a scisso ...200919653676
stenotrophomonas maltophilia drug resistance.stenotrophomonas maltophilia has emerged in recent years as a paradigm of an intrinsically resistant, opportunistic bacterial pathogen with an environmental origin. the recent publication of the sequences of two s. maltophilia genomes has shown that this bacterium contains a large repertoire of antibiotic resistance determinants, probably contributing to its characteristic susceptibility to antibiotics. among those determinants, the best characterized are a number of multidrug efflux pumps, beta ...200919659422
[effect of nickel ions on physiological and corrosion activity of bacteria of sulfur cycle].the paper deals with the effect of nickel ions concentration in nutrient medium on sulfate-reducing bacteria desulfovibrio desulfuricans 10-v (srb) and their artificial corrosion-active associations which included thionic bacteria and their satellite stenotrophomonas maltophilia. it is shown that the concentration of nickel in the nutrient medium being increased, the duration of lag-phase of srb growth became 2-2.5 times less, and that of artificial associations--3-3.5 times less. the specific g ...200919663327
antibacterial activities of a fosfomycin/tobramycin combination: a novel inhaled antibiotic for bronchiectasis.to compare the in vitro and in vivo activities of a 4:1 (w/w) fosfomycin/tobramycin combination (fti) with those of fosfomycin and tobramycin alone against cystic fibrosis (cf) and non-cf bronchiectasis pathogens.200919679597
local adaptation of bacteriophages to their bacterial hosts in soil.microbes are incredibly abundant and diverse and are key to ecosystem functioning, yet relatively little is known about the ecological and evolutionary mechanisms that shape their distributions. bacteriophages, viral parasites that lyse their bacterial hosts, exert intense and spatially varying selection pressures on bacteria and vice versa. we measured local adaptation of bacteria and their associated phages in a centimeter-scale soil population. we first demonstrate that a large proportion of ...200919679806
stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field.a gram-negative, non-spore-forming, rod-shaped bacterium, designated strain dcy01(t), was isolated from soil from a ginseng field in south korea and was characterized in order to determine its taxonomic position. 16s rrna gene sequence analysis revealed that strain dcy01(t) belonged to the gammaproteobacteria and was most closely related to stenotrophomonas koreensis kctc 12211(t) (98.4 % similarity), stenotrophomonas humi r-32729(t) (97.2 %), stenotrophomonas terrae r-32768 (97.1 %), stenotroph ...201019684314
comparison of two multimetal resistant bacterial strains: enterobacter sp. ysu and stenotrophomonas maltophilia oro2.the y-12 plant in oak ridge, tn, which manufactured nuclear weapons during world war ii and the cold war, contaminated east fork poplar creek with heavy metals. the multimetal resistant bacterial strain, stenotrophomonas maltophilia oak ridge strain o2 (s. maltophilia o2), was isolated from east fork poplar creek. sequence analysis of 16s rdna suggested that our working strain of s. maltophilia o2 was a strain of enterobacter. phylogenetic tree analysis and biochemical tests confirmed that it be ...200919688378
accuracy of american thoracic society/infectious diseases society of america criteria in predicting infection or colonization with multidrug-resistant bacteria at intensive-care unit admission.the aim of this prospective observational study was to determine the accuracy of american thoracic society (ats)/infectious diseases society of america (idsa) criteria in predicting infection or colonization related to multidrug-resistant (mdr) bacteria at intensive-care unit (icu) admission. mdr bacteria were defined as methicillin-resistant staphylococcus aureus, ceftazidime-resistant or imipenem-resistant pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, and exten ...201019694760
infections following lawnmower and farm machinery-related injuries in children.because of the paucity of previous data, we report the organisms causing infection in children with injuries from lawnmowers and farm machinery.200919702605
stenotrophomonas and lysobacter: ubiquitous plant-associated gamma-proteobacteria of developing significance in applied microbiology.the exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in lysobacter, 11 new species having been described since 2005, and in stenotrophomonas with six new species since 2000. the new species of lysobacter, isolated by dilution and direct plating on standard media, differ in several key phenotypic properties from those obtained by enrichment on complex polysaccharides in the original descr ...201019702860
recurrent hemoptysis with penicillium marneffei and stenotrophomonas maltophilia in job's syndrome.pulmonary infection caused by the opportunistic organisms penicillium marneffei and stenotrophomonas maltophilia in patients with job's syndrome is rare and not well documented. the case of a 30-year-old man with job's syndrome who developed recurrent pneumonia and lung abscesses caused by p. marneffei and s. maltophilia, complicated by massive hemoptysis, is described. bronchial artery embolization was successful in controlling the hemoptysis; however, the infection proved fatal despite appropr ...200919707602
endophytic nitrogen fixation in dune grasses (ammophila arenaria and elymus mollis) from oregon.several tropical grasses harbor symbiotic nitrogen-fixing bacteria within their stem and rhizome tissue that may contribute to the nitrogen nutrition of the host plant. we present evidence here that sand dune grasses (ammophila arenaria and elymus mollis) from oregon also contain nitrogen-fixing bacteria. surface-sterilized stem and rhizome tissue from these species possess acetylene reduction (nitrogen fixation) activity and large populations (10(5) to 10(6) cfu/g fresh weight) of bacteria. the ...200419712295
a novel isoquinoline alkaloid, dd-carboxypeptidase inhibitor, with antibacterial activity isolated from streptomyces sp. 8812. part i: taxonomy, fermentation, isolation and biological activities.a novel isoquinoline alkaloid of molecular formula c10h9no4, labeled js-1, was isolated from the culture broth of streptomyces sp. 8812. it was purified by acetone protein precipitation from the culture supernatant, followed by anion exchange and c18 rp hplc columns. js-1 is an inhibitor of exocellular dd-carboxypeptidases/transpeptidases (dd-peptidases) 64-575 ii from saccharopolyspora erythraea 64-575 ii, and r39 from actinomadura r39. js-1 exhibits activity against gram-negative bacteria, suc ...200919713994
fulminant pertussis: a multi-center study with new insights into the clinico-pathological mechanisms.pertussis carries a high risk of mortality in very young infants. the mechanism of refractory cardio-respiratory failure is complex and not clearly delineated. we aimed to examine the clinico-pathological features and suggest how they may be related to outcome, by multi-center review of clinical records and post-mortem findings of 10 patients with fulminant pertussis (fp). all cases were less than 8 weeks of age, and required ventilation for worsening respiratory symptoms and inotropic support f ...200919725100
nitrofurantoin-induced systemic inflammatory response syndrome.nitrofurantoin is a commonly used antibiotic for the prophylaxis and treatment of urinary tract infections. we report the first case of nitrofurantoin-induced systemic inflammatory response syndrome, an important adverse drug reaction in the setting of what was initially believed to be a recurrent infection.200919726971
trends in pathogens colonising the respiratory tract of adult patients with cystic fibrosis, 1985-2005.the treatment of patients with cf has continued to evolve. we hypothesised that sputum microbiology may have changed as a result of this.200919740710
multidrug-resistant gram-negative infections: what are the treatment options?the emergence of multidrug-resistant (mdr) gram-negative bacilli creates a challenge in the treatment of nosocomial infections. while the pharmaceutical pipeline is waning, two revived old antibacterials (colistin and fosfomycin), a newer one (tigecycline) and an 'improved' member of an existing class (doripenem) are the only therapeutic options left. the class of polymyxins, known since 1947 and represented mostly by polymyxin b and polymyxin e (colistin), has recently gained a principal role i ...200919747006
impact of cadmium on the bacterial communities in the gut of metaphire posthuma.the effects of cadmium (cd) contamination in soil onto the bacterial communities of the guts pooled from ten metaphire posthuma were addressed during 14 days' incubation. we found that about 50% of cd (5mg/kg, dry weight soil) in the contaminated soil was bio-accumulated into the earthworms. dna was extracted from the guts of m. posthuma and their dwelling soil irrespective of cd treatment for the analysis of the bacterial communities of guts in m. posthuma and in soil by pcr-dgge (polymerase ch ...200919747774
combination of chromogenic differential medium and esta-specific pcr for isolation and detection of phytopathogenic xanthomonas spp.a xanthomonad differential medium (designated xan-d medium) was developed, on which streaks and colonies of xanthomonads, including 13 species of the genus xanthomonas, turned wet-shining yellow-green and were surrounded with a smaller milky zone and a bigger clear zone in 3 to 4 days. the characteristics could easily be differentiated from those of yellow nonxanthomonads and other bacteria. the mechanism of color change and formation of a milky zone on the medium are mainly due to the tween 80 ...200919749062
the antibacterial properties of malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey.antibiotic resistance of bacteria is on the rise, thus the discovery of alternative therapeutic agents is urgently needed. honey possesses therapeutic potential, including wound healing properties and antimicrobial activity. although the antimicrobial activity of honey has been effectively established against an extensive spectrum of microorganisms, it differs depending on the type of honey. to date, no extensive studies of the antibacterial properties of tualang (koompassia excelsa) honey on wo ...200919754926
seasonal variation in detection of bacterial dna in arthritic stifle joints of dogs with cranial cruciate ligament rupture using pcr amplification of the 16s rrna gene.an underappreciated cause and effect relationship between environmental bacteria and arthritis may exist. previously, we found that stifle arthritis in dogs was associated with the presence of environmental bacteria within synovium. cranial cruciate ligament rupture (cclr) is often associated with stifle arthritis in dogs. we now wished to determine whether seasonal variation in detection of bacterial material may exist in affected dogs, and to also conduct analyses of both synovium and synovial ...201019758772
association of stenotrophomonas maltophilia infection with lower airway disease in the horse: a retrospective case series.stenotrophomonas maltophilia is being reported with increasing frequency as a human nosocomial pathogen, especially among immuno-compromised patients. to the authors' knowledge, this pathogen has not previously been associated with lower airway disease in the horse. in this paper the clinical findings, laboratory diagnosis and response to treatment of seven cases of respiratory infection with s. maltophilia in horses, presented at three equine referral hospitals in denmark in 2007, are described ...201019758829
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