Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
isolation and selection of yeasts from wine grape ecosystem secreting cold-active pectinolytic activity.the present study was undertaken with the purpose of selecting yeasts from wine grapes that are able to produce extracellular cold-active pectinases. after two consecutive selections yeast isolates were identified by pheno- and genotyping, and pectinolytic activity was preliminarily characterised at proximate winemaking conditions. out of 1023 indigenous microorganisms isolated from grape skins of d.o. san rafael (mendoza, argentina) viticulture region, 565 (55%) showed pectinolytic activity on ...201121529977
physicochemical, nutritional, and antimicrobial properties of wine grape (cv. merlot) pomace extract-based films.wine grape pomace (wgp) (cv. merlot) extract-based films were studied in terms of their physicochemical, mechanical, water barrier, nutritional, and antibacterial properties. pomace extract (pe) was obtained by hot water extraction and had a total soluble solid of 3.6% and ph 3.65. plant-based polysaccharides, low methoxyl pectin (lmp, 0.75% w/w), sodium alginate (sa, 0.3% w/w), or ticafilm (tf, 2% w/w), was added into pe for film formation, respectively. elongation at break and tensile strength ...201121535831
oviposition site preferences and performance in natural resources in the human commensals drosophila melanogaster and d. simulans.the choice of egg laying site and progeny's performance in a rearing site are important components of habitat selection. despite the huge amount of genetic, morphological, behavioral and physiological data regarding drosophila melanogaster meigen and d. simulans sturtevant, oviposition site preferences remain poorly known. we investigated resource preference (acceptance and choice) and performance (measured as larval viability, developmental time and wing size) in vitis vinifera linneo (grape) a ...201121540639
acyl substrate preferences of an iaa-amido synthetase account for variations in grape (vitis vinifera l.) berry ripening caused by different auxinic compounds indicating the importance of auxin conjugation in plant development.nine gretchen hagen (gh3) genes were identified in grapevine (vitis vinifera l.) and six of these were predicted on the basis of protein sequence similarity to act as indole-3-acetic acid (iaa)-amido synthetases. the activity of these enzymes is thought to be important in controlling free iaa levels and one auxin-inducible grapevine gh3 protein, gh3-1, has previously been implicated in the berry ripening process. ex planta assays showed that the expression of only one other gh3 gene, gh3-2, incr ...201121543520
xylem structure of four grape varieties and 12 alternative hosts to the xylem-limited bacterium xylella fastidious.background and aims the bacterium xylella fastidiosa (xf), responsible for pierce's disease (pd) of grapevine, colonizes the xylem conduits of vines, ultimately killing the plant. however, vitis vinifera grapevine varieties differ in their susceptibility to xf and numerous other plant species tolerate xf populations without showing symptoms. the aim of this study was to examine the xylem structure of grapevines with different susceptibilities to xf infection, as well as the xylem structure of no ...201121546428
association of a novel dna virus with the grapevine vein-clearing and vine decline syndrome.a severe vein-clearing and vine decline syndrome has emerged on grapevines (vitis vinifera) and hybrid grape cultivars in the midwest region of the united states. the typical symptoms are translucent vein-clearing on young leaves, short internodes and decline of vine vigor. known viral pathogens of grapevines were not closely associated with the syndrome. to obtain a comprehensive profile of viruses in a diseased grapevine, small rnas were enriched and two cdna libraries were constructed from a ...201121554183
expression and genome-wide analysis of the xylogen-type gene family.in higher plants, many extracellular proteins are involved in developmental processes, including cell-cell signaling and cell wall construction. xylogen is an extracellular arabinogalactan protein (agp) isolated from zinnia elegans xylogenic culture medium, which promotes xylem cell differentiation. xylogen has a unique structure, containing a non-specific lipid transfer protein (nsltp) domain and agp domains. we searched for xylogen-type genes in the genomes of land plants, including arabidopsi ...201121558309
evolution of phenolic composition of red wine during vinification and storage and its contribution to wine sensory properties and antioxidant activity.the objective of this work was to study the evolution of the phenolic composition of red wine during vinification and storage and its relationship with some sensory properties (astringency and bitterness) and antioxidant activities. thus, red wine was made by a classic vinification method with castelão and tinta miúda grapes (vitis vinifera l.) harvested at maturity (3:2; w/w). samples were taken at 2 and 7 days of maceration, at second racking, at the time of bottling and at 6 and 14 months aft ...201121561162
inflorescence of grapevine (vitis vinifera l.): a high ability to distribute its own assimilates.the distribution of carbon (c) into whole grapevine fruiting cuttings was investigated during flower development to determine the relative contribution of inflorescence and leaf photoassimilates in the total c balance and to investigate their partitioning towards other plant organs. a (13)c labelling procedure was used to label c photoassimilates by leaves and inflorescences in grapevine. investigations were carried out at various stages of flower/berry development, from separated cluster to fru ...201121561954
auxin-nonresponsive grape aux/iaa19 is a positive regulator of plant growth.we report the characterization of vviaa19, an auxin/indole-3-acetic acid (aux/iaa) protein, in grapevine (vitis vinifera l.). vviaa19 was expressed abundantly in berries. vviaa19 transcription was rapidly increased at pre-anthesis and then decreased during fruit set. before véraison, however, vviaa19 gene expression was upregulated again and maximum expression was maintained until the end of ripening. exogenous iaa did not induce vviaa19 expression in grape leaves, suggesting that vviaa19 might ...201221562765
automated analysis of three-dimensional xylem networks using high-resolution computed tomography.connections between xylem vessels represent important links in the vascular network, but the complexity of three-dimensional (3d) organization has been difficult to access. this study describes the development of a custom software package called tanax (tomography-derived automated network analysis of xylem) that automatically extracts vessel dimensions and the distribution of intervessel connections from high-resolution computed tomography scans of grapevine (vitis vinifera) stems, although the ...201121569032
a dige-based quantitative proteomic analysis of grape berry flesh development and ripening reveals key events in sugar and organic acid metabolism.grapevine (vitis vinifera l.) is an economically important fruit crop. quality-determining grape components, such as sugars, acids, flavours, anthocyanins, tannins, etc., are accumulated during the different grape berry development stages. thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in grape is of paramount importance to advance the understanding of the berry development and ripening processes. here, the developmental analysis of v. vinifera ...201121576399
from vineyard to glass: agrochemicals enhance the melatonin and total polyphenol contents and antiradical activity of red wines.resistance inducers are a class of agrochemicals, including benzothiadiazole and chitosan, which activate the plant own defence mechanisms. in this work, open-field treatments with plant activators were performed on two red grape (vitis vinifera l.) varieties cultivated in different localities, groppello (brescia, lombardia, italy) and merlot (treviso, veneto, italy). treatments were carried out every 10 days until the véraison and, after harvesting, experimental wines (microvinificates) were pr ...201121585520
fruit ripening in vitis vinifera: spatiotemporal relationships among turgor, sugar accumulation, and anthocyanin biosynthesis.this study reports the first observations indicating the spatiotemporal relationships among genetic and physiological aspects of ripening in the berry of vitis vinifera. at the onset of ripening in the red flesh variety alicante bouschet, colour development began in the flesh at the stylar end of the fruit and progressed toward the pedicel end flesh and into the skin. tissue solute potential and cell turgor also decreased first in the flesh. the decrease in flesh solute potential was due to accu ...201121586429
biosynthesis of methoxypyrazines: elucidating the structural/functional relationship of two vitis viniferao-methyltransferases capable of catalyzing the putative final step of the biosynthesis of 3-alkyl-2-methoxypyrazine.3-alkyl-2-methoxypyrazines (mps) are an important food constituent and have been associated with detrimental herbaceous flavors in red wines by consumers and the wine industry. the vitis vinifera genes o-methyltransferase 1 and 2 (vvomt1 and vvomt2) have been isolated in the grapevine cultivar carmenere. these genes encode s-adenosyl-l-methionine (sam)-dependent o-methyltransferases, which have the ability to methylate 3-alkyl-2-hydroxypyrazines (hps)-the putative final step in mps production. a ...201121591781
identification of tissue-specific, abiotic stress-responsive gene expression patterns in wine grape (vitis vinifera l.) based on curation and mining of large-scale est data sets.abiotic stresses, such as water deficit and soil salinity, result in changes in physiology, nutrient use, and vegetative growth in vines, and ultimately, yield and flavor in berries of wine grape, vitis vinifera l. large-scale expressed sequence tags (ests) were generated, curated, and analyzed to identify major genetic determinants responsible for stress-adaptive responses. although roots serve as the first site of perception and/or injury for many types of abiotic stress, est sequencing in roo ...201121592389
anticoagulant effect of polyphenols-rich extracts from black chokeberry and grape seeds.blood coagulation consists of a series of zymogens that can be converted by limited proteolysis to active enzymes leading to the generation of thrombin. fresh plasma and human thrombin was incubated with extracts from berries of aronia melanocarpa or seeds of vitis vinifera (0.5; 5; 50μg/ml). the in vitro experiments showed that both extracts prolonged clotting time and decreased the maximal velocity of fibrin polymerization in human plasma. moreover thrombin incubation with both extracts result ...201121596108
melatonin levels, determined by lc-esi-ms/ms, fluctuate during the day/night cycle in vitis vinifera cv malbec: evidence of its antioxidant role in fruits.the identification of melatonin in plants has inspired new investigations to understand its biological function and which endogenous and external factors control its levels in these organisms. owing to the therapeutical and nutraceutical properties of melatonin, it should be important to develop reliable analytical methods for its quantification in vegetal matrices containing this indoleamine, such as grape and wine. the main objectives of the present study were to test whether melatonin levels ...201121605162
in vivo grapevine anthocyanin transport involves vesicle-mediated trafficking and the contribution of anthomate transporters and gst.in cells, anthocyanin pigments are synthesized at the cytoplasmic surface of the endoplasmic reticulum, and are then transported and finally accumulated inside the vacuole. in vitis vinifera (grapevine), two kinds of molecular actors are putatively associated with the vacuolar sequestration of anthocyanins: a glutathione-s-transferase (gst) and two mate-type transporters, named anthomates. however, the sequence of events by which anthocyanins are imported into the vacuole remains unclear. we use ...201121605207
the presence of melatonin in grapevine (vitis vinifera l.) berry tissues.melatonin has been reported in a variety of food plants and, consequently, in a number of plant-derived foodstuffs. in grapevine (vitis vinifera l.) products, it was found in berry exocarp (skin) of different cultivars and monovarietal wines. herein, we assessed, by means of mass spectrometry, the occurrence of melatonin in all berry tissues (skin, flesh, and seed) at two different phenological stages, pre-véraison and véraison. we detected the highest melatonin content in skin, at pre-véraison, ...201121615489
molecular phylogeny of the genus vitis (vitaceae) based on plastid markers. 201021616868
a physical map for the amborella trichopoda genome sheds light on the evolution of angiosperm genome structure.recent phylogenetic analyses have identified amborella trichopoda, an understory tree species endemic to the forests of new caledonia, as sister to a clade including all other known flowering plant species. the amborella genome is a unique reference for understanding the evolution of angiosperm genomes because it can serve as an outgroup to root comparative analyses. a physical map, bac end sequences and sample shotgun sequences provide a first view of the 870 mbp amborella genome.201121619600
restructuring of endophytic bacterial communities in grapevine yellows-diseased and recovered vitis vinifera l. plants.length heterogeneity-pcr assays, combined with statistical analyses, highlighted that the endophytic bacterial community associated with healthy grapevines was characterized by a greater diversity than that present in diseased and recovered plants. the findings suggest that phytoplasmas can restructure the bacterial community by selecting endophytic strains that could elicit a plant defense response.201121622794
wound-induced vascular occlusions in vitis vinifera (vitaceae): tyloses in summer and gels in winter1.vascular occlusion in xylem conduits is a common response to environmental stresses, and plant species are recognized as primarily tylose-forming or gel-forming. these stresses occur throughout the year, but there is little information on the wound responses throughout the year and in growing and dormant tissues. wound-induced vascular occlusions were evaluated by type (tylose or gel), temporal progress, and spatial distribution for grape stems pruned in four seasons through an entire year. tylo ...200821628157
accumulation and distribution pattern of macro- and microelements and trace elements in vitis vinifera l. cv. chardonnay berries.this paper describes the accumulation pattern of 42 mineral elements in vitis vinifera l. berries during development and ripening and their distribution in berry skin, seeds, and flesh around harvest time. grape berries were sampled in two different vineyards with alkaline soil and analyzed using a icp-ms. although elemental amounts were significantly different in the grapes from the two vineyards, the accumulation pattern and percentage distribution in different parts of the berries were genera ...201121639148
pruning-induced tylose development in stems of current-year shoots of vitis vinifera (vitaceae).tyloses form in xylem vessels in response to various environmental stimuli, but little is known of the kinetics or regulation of their development. preliminary investigations indicated that wounds seal quickly with tyloses after pruning of grapevine shoots. in this study, tylose development was analyzed qualitatively and quantitatively at different depths and times from pruning cuts along current-year shoots of grapevines at basal, middle, and apical stem regions. tyloses developed simultaneousl ...200621642102
analysis of bac end sequences in oak, a keystone forest tree species, providing insight into the composition of its genome.one of the key goals of oak genomics research is to identify genes of adaptive significance. this information may help to improve the conservation of adaptive genetic variation and the management of forests to increase their health and productivity. deep-coverage large-insert genomic libraries are a crucial tool for attaining this objective. we report herein the construction of a bac library for quercus robur, its characterization and an analysis of bac end sequences.201121645357
the structure of xylem vessels in grapevine (vitaceae) and a possible passive mechanism for the systemic spread of bacterial disease.xylem-dwelling pathogens become systemic, suggesting that microorganisms move efficiently in the xylem. to better understand xylem pathways and how bacteria move within the xylem, vessel connectivity between stems and leaves of vitis vinifera cv. chardonnay and muscadinia rotundifolia cv. cowart was studied. three methods were used: (1) the light-producing bacterium, yersinia enterocolitica, (ye) strain gy5232 was loaded into petioles and followed using x-ray film, (2) fluorescent beads were loa ...200621646209
the role of light in the regulation of ascorbate metabolism during berry development in the cultivated grapevine vitis vinifera l.the accumulation of l-ascorbate (asc) in fruits is influenced by environmental factors including light quantity. fruit exposure to ambient light is often reduced by the surrounding leaf canopy, and can be altered by cultivation practices. the influence of reduced sunlight exposure on the accumulation of asc and its catabolites was investigated in field-grown berries of the cultivated grapevine vitis vinifera l. cv. shiraz.201121656772
comparative anatomy and morphology of vitis vinifera (vitaceae) somatic embryos from solid- and liquid-culture-derived proembryogenic masses.ontogeny of somatic embryos of grapevine (vitis vinifera) produced from solid- and liquid-culture-derived proembryogenic masses (pem) was compared using light and scanning electron microscopy. somatic embryos produced from solid-medium-derived pem (spem) had large cotyledons, little or no visible suspensor structure, and a relatively undeveloped concave shoot apical meristem, whereas those from liquid-medium-derived pem (lpem) had smaller cotyledons, a distinct suspensor, and a flat-to-convex sh ...200321659195
incidence of fumonisin b(2) production by aspergillus niger in portuguese wine regions.fumonisin b(2) (fb(2)) was recently found to be produced by aspergillus niger . when grape-derived products were subsequently analyzed, fb(2) contamination was found in raisins, must, and wine. this study evaluated 681 strains of black aspergilli species isolated from portuguese wine grapes for fb(2) production when grown on czapek yeast agar. fb(2) was not detected in aspergillus carbonarius (n = 75) or aspergillus ibericus (n = 9) strains, but it was detected in 176 (29%) of the strains belong ...201121668017
the application of ssrs characterized for grape (vitis vinifera) to conservation studies in vitaceae.the use of microsatellite loci developed from a single plant species across a number of related taxa is becoming increasingly widespread. this approach can provide highly informative markers even for species for which microsatellites have not been characterized. as a number of expressed sequence tag (est)-derived and enrichment-derived microsatellites are available for grape (vitis vinifera), this study set out to assess transferability of nine such loci across 25 species from five different vit ...200221669708
extensin network formation in vitis vinifera callus cells is an essential and causal event in rapid and h(2)o(2)-induced reduction in primary cell wall hydration.extensin deposition is considered important for the correct assembly and biophysical properties of primary cell walls, with consequences to plant resistance to pathogens, tissue morphology, cell adhesion and extension growth. however, evidence for a direct and causal role for the extensin network formation in changes to cell wall properties has been lacking.201121672244
transcriptome analysis of sarracenia, an insectivorous plant.sarracenia species (pitcher plants) are carnivorous plants which obtain a portion of their nutrients from insects captured in the pitchers. to investigate these plants, we sequenced the transcriptome of two species, sarracenia psittacina and sarracenia purpurea, using roche 454 pyrosequencing technology. we obtained 46 275 and 36 681 contigs by de novo assembly methods for s. psittacina and s. purpurea, respectively, and further identified 16 163 orthologous contigs between them. estimation of s ...201121676972
epigenetic repressor-like genes are differentially regulated during grapevine (vitis vinifera l.) development.grapevine sexual reproduction involves a seasonal separation between inflorescence primordia (flowering induction) and flower development. we hypothesized that a repression mechanism implicating epigenetic changes could play a role in the seasonal separation of these two developmental processes in grapevine. therefore, the expression of five grapevine genes with homology to the arabidopsis epigenetic repressor genes fertilization independent endosperm (fie), embryonic flower 2 (emf2), curly leaf ...201121681473
inter- and intra-varietal genetic variability in malvasia cultivars.the dna molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the vitis vinifera l. cultivars and clones. ten malvasia cultivars (i.e., istrian malvasia; m. delle lipari; m. bianca di candia; m. di candia aromatica; m. del lazio; m. bianca lunga, also known as malvasia del chianti; m. nera di brindisi/lecce; m. di casorzo; m. di schierano, and m. nera di bolzano) were analyzed using m ...201221688040
sulphur dioxide evokes a large scale reprogramming of the grape berry transcriptome associated with oxidative signalling and biotic defence responses.the grape and wine industries are heavily reliant on sulphite preservatives. however, the view that sulphites act directly on bacterial and fungal pathogens may be simplistic. mechanisms of sulphur-enhanced defences are largely unknown; many sulphur-rich compounds enhance plant defences and sulphite can also have oxidative consequences via production of h(2)o(2) or sulphitolysis. to investigate the effects of sulphur dioxide (so(2) ) on fresh table grapes (vitis vinifera l. 'crimson seedless'), ...201221689113
metabolic disorders and oxidative stress programming in offspring of rats fed a high-fat diet during lactation: effects of a vinifera grape skin (ach09) extract.this study examined the effect of vitis vinifera grape skin ach09 extract (ach09) on metabolic disorders and oxidative stress in adult offspring of rats fed a high-fat diet (hf) during lactation. four groups of female rats were fed: control diet (7% fat); ach09 (7% fat + 200 mg-àkg-àd ach09 orally); hf (24% fat); hf+ ach09 (24% fat + 200 mg-àkg-àd ach09 orally) during lactation. from weaning onward, all female offspring were fed a control diet and killed when they were 90 or 180 days old. systol ...201121697730
relationship between red wine grade and phenolics. 2. tannin composition and size.commercial red wines ( vitis vinifera l. cv. shiraz) produced during the 2009 vintage underwent winemaker assessment for allocation grade soon after production. the wines were then subjected to phenolic analysis to measure wine color (total anthocyanin, so(2) nonbleachable pigment, and wine color density) and tannins (concentration, composition, and average degree of polymerization). a positive relationship was found between wine phenolic concentration and projected bottle price. tannin composit ...201121702478
mehercules, adhuc bacchus! the debate on wine proteomics continues.the proteome of untreated white wines (a recioto made with garganega grapes from the veneto region) was explored in depth via capture with combinatorial peptide ligand libraries (cpll) at four different ph values: ph 2.2, 3.8, 7.2, and 9.3. the combined data on the discoveries in the four cpll eluates, as well as in the collected bottle sediment, allowed the identification of 106 unique gene products belonging to vitis vinifera, as well as of an additional 11 proteins released by the s. cerevisi ...201121702502
the susceptibility of small fruits and cherries to the spotted-wing drosophila, drosophila suzukii.the spotted-wing drosophila, drosophila suzukii matsumura, is native to asia and was first detected in the north american mainland and europe in 2008-2010. drosophila suzukii is a serious economic pest to stone and small fruits because the female lays eggs within ripening fruit on a plant before harvest, which can lead to crop loss. the aim of this study was to evaluate the susceptibility of blackberries, blueberries, cherries, grapes, raspberries and strawberries to d. suzukii among various rip ...201121710685
phenolic composition of the brazilian seedless table grape varieties brs clara and brs morena.the detailed phenolic composition (anthocyanins, flavonols, hydroxycinnamic acid derivatives, stilbenes, and flavan-3-ols) in the skin and flesh of the new brs clara and brs morena seedless table grapes has been studied using hplc-dad-esi-ms/ms. the two grapes, especially brs morena, contained high amounts of phenolic compounds, mainly located in their skins and qualitatively not different from those found in vitis vinifera grapes. in addition, brs morena (a teinturier variety) showed qualitativ ...201121714563
stilbenes and tyrosol as target compounds in the assessment of antioxidant and hypolipidemic activity of vitis vinifera red wines from southern brazil.the contents of stilbene monomers, cis-resveratrol, trans-resveratrol, cis-piceid, trans-piceid, and tyrosol, were quantified in vitis vinifera red wines, cvs. cabernet franc, merlot, sangiovese, and syrah, 2006 and 2007 vintages, from the sa¦âo joaquim region, a new grape-growing region at southern brazil. moreover, the effect of chronic consumption of these wines on the antioxidant and hypolipidemic activities was monitored in c57bl6 ldl receptor knockout mice and treated with a hypercholester ...201121718033
determination of the geographical origin of brazilian wines by isotope and mineral analysis.in the present research, we studied wines from three different south brazilian winemaking regions with the purpose of differentiating them by geographical origin of the grapes. brazil's wide territory and climate diversity allow grape cultivation and winemaking in many regions of different and unique characteristics. the wine grape cultivation for winemaking concentrates in the south region, mainly in the serra gaúcha, the mountain area of the state of rio grande do sul, which is responsible for ...201121720735
host plant volatiles induce oriented flight behaviour in male european grapevine moths, lobesia botrana.the european grapevine moth lobesia botrana relies on a female produced sex pheromone for long-distance mate finding. grapevine moth males compete heavily during limited time windows for females. the aim of this study was to investigate the perception of host plant volatiles by grapevine moth males and whether such compounds elicit upwind oriented flights. we compared five host plant headspace extracts by means of gas chromatography linked electroantennogram (eag) recording. we identified 12 com ...201121729701
a white spruce gene catalog for conifer genome analyses.several angiosperm plant genomes, including arabidopsis (arabidopsis thaliana), rice (oryza sativa), poplar (populus trichocarpa), and grapevine (vitis vinifera), have been sequenced, but the lack of reference genomes in gymnosperm phyla reduces our understanding of plant evolution and restricts the potential impacts of genomics research. a gene catalog was developed for the conifer tree picea glauca (white spruce) through large-scale expressed sequence tag sequencing and full-length cdna sequen ...201121730200
isolation, identification and sequence analysis of a thioredoxin h gene, a member of subgroup iii of h-type trxs from grape (vitis vinifera l. cv. askari).thioredoxins (trxs) are small ubiquitous proteins which play a regulatory role in a variety of cellular processes. in contrast to other organisms, plants have a great number of trx types, consisting of six well-defined groups: f, m, x, and y in chloroplasts, o in mitochondria, and h mainly in cytosol. a full-length cdna, designated vvcxxs2, encoding trx h polypeptide was isolated and cloned from grape (vitis vinifera l. cv. askari) berries organ by reverse transcription polymerase chain reaction ...201121732058
hepatoprotective and antioxidant activities of grapeseeds against ethanol-induced oxidative stress in rats.the present study was carried out to evaluate the hepatoprotective effect and antioxidant role of grape (vitis vinifera l.) seeds (gs) against ethanol-induced oxidative stress. the hepatoprotective and antioxidant roles of the gs supplementation feed against ethanol-induced oxidative stress were evaluated by measuring liver damage serum marker enzymes, aspartate aminotransferase, alanine aminotransferase, +¦-glutamyl transpeptidase and lactate dehydrogenase, antioxidant defence system such as gs ...201121733325
effects of elicitors on the production of resveratrol and viniferins in cell cultures of vitis vinifera l. cv italia.methyl jasmonate, jasmonic acid and chitosan were tested as elicitors on cell suspension cultures obtained from vitis vinifera cv italia to investigate their effect on stilbene production. stilbene accumulation in the callus, grown under nonelicited conditions, was also investigated. calli and cell suspensions were obtained in a b5 culture medium supplemented with 0.2 mg l(-1) naa and 1 mg l(-1) kin. stilbene determination was achieved by hplc/dad/ms. whereas callus biosynthesized only piceid, c ...201121751812
resveratrol ameliorates tnfa-mediated suppression of erythropoiesis in human cd34(+) cells via modulation of nf-?b signalling.overexpression of pro-inflammatory cytokines, including tumour necrosis factor alpha (tnfa), has been implicated in the pathogenesis of anaemia of inflammation. tnfa suppresses erythroid colony formation via both direct and indirect effects on haematopoietic progenitors, often involving activation of nuclear factor (nf)-?b signalling resulting in downregulation of transcription factors critical for erythropoiesis. there is a dearth of effective and safe therapies for many patients with inflammat ...201121762122
investigations on the leaf anatomy and ultrastructure of grapevine (vitis vinifera) under heat stress.leaf anatomical and ultrastructural responses of "razegui" and "muscat italia" grapevine cultivars to high temperatures were studied under controlled conditions (t > 36°c), based on photonic and electron microscopy. histological studies performed on leaves from heat-stressed and control grapevines revealed thicker leaf blades under high temperature conditions. environmental scanning electron microscopy of leaf surfaces from both cultivars allowed observing sinuate epidermal cells on the leaves o ...201121780249
compatible glrav-3 viral infections affect berry ripening decreasing sugar accumulation and anthocyanin biosynthesis in vitis vinifera.virus infections in grapevine cause important economic losses and affect fruit quality worldwide. although the phenotypic symptoms associated to viral infections have been described, the molecular plant response triggered by virus infection is still poorly understood in vitis vinifera. as a first step to understand the fruit changes and mechanisms involved in the compatible grapevine-virus interaction, we analyzed the berry transcriptome in two stages of development in the red wine cultivar cabe ...201121786204
molecular cloning and expression of gene encoding aromatic amino acid decarboxylase in 'vidal blanc' grape berries.the pleasantly fruity and floral 2-phenylethanol are a dominant aroma compound in post-ripening 'vidal blanc' grapes. however, to date little has been reported about its synthetic pathway in grapevine. in the present study, a full-length cdna of vvaadc (encoding aromatic amino acid decarboxylase) was firstly cloned from the berries of 'vidal blanc', an interspecific hybrid variety of vitis vinifera × vitis riparia. this sequence encodes a complete open reading frame of 482 amino acids with a cal ...201221789483
volatiles that encode host-plant quality in the grapevine moth.plant volatiles are signals used by herbivorous insects to locate host plants and select oviposition sites. whether such volatiles are used as indicators of plant quality by adult insects in search of host plants has been rarely tested. we tested whether volatiles indicate plant quality by studying the oviposition of the grapevine moth lobesia botrana on the grapevine plant vitis vinifera. host plants were infected with a variety of microorganisms, and larval fitness was correlated to the infect ...201121794883
anthocyanin composition and extractability in berry skin and wine of vitis vinifera l. cv. aglianico.the present article reports the anthocyanin content in the berry skin and wine of the italian red grape cultivar aglianico (clone vcr11 grafted onto 1103 paulsen), one of the most ancient vines and famous for its deep-red colour. anthocyanins were extracted from frozen berry skin in an acidified methanol solution. the extraction mixtures, monitored for 120 h, were analysed by high-performance liquid chromatography.201121800322
isolation and expression analysis of salt induced genes from contrasting grapevine (vitis vinifera l.) cultivars.salt stress adversely affects the growth of grapevine plants. in order to understand the molecular basis of salt stress response in grapevine plants, suppression subtractive hybridization (ssh) and microarray based screening approaches were combined. two leaf-specific subtractive cdna libraries were constructed from grapevine plants subjected to a moderate, incremental salt stress treatment. ssh were performed 6h and 24h after nacl peaked at 100mm using cdnas prepared from leaves of a salt toler ...201021802607
identification and expression analysis of genes associated with the early berry development in the seedless grapevine (vitis vinifera l.) cultivar sultanine.sultanine grapevine (vitis vinifera l.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. actually, studies concerning berry development in seedless grapes are scarce at the molecular level. we therefore developed a re ...201021802609
unsupervised principal component analysis of nmr metabolic profiles for the assessment of substantial equivalence of transgenic grapes (vitis vinifera).substantial equivalence is a key concept in the evaluation of unintended and potentially harmful metabolic impact consequent to a genetic modification of food. the application of unsupervised multivariate data analysis to the metabolic profiles is expected to improve the effectiveness of such evaluation. the present study uses nmr spectra of hydroalcoholic extracts, as holistic representations of the metabolic profiles of grapes, to evaluate the effect of the insertion of one or three copies of ...201121806070
optimisation of solid-phase microextraction combined with gas chromatography-mass spectrometry based methodology to establish the global volatile signature in pulp and skin of vitis vinifera l. grape varieties.the volatiles (vocs) and semi-volatile organic compounds (svocs) responsible for aroma are mainly present in skin of grape varieties. thus, the present investigation is directed towards the optimisation of a solvent free methodology based on headspace-solid-phase microextraction (hs-spme) combined with gas chromatography-quadrupole mass spectrometry (gc-qms) in order to establish the global volatile composition in pulp and skin of bual and bastardo vitis vinifera l. varieties. a deep study on th ...201121807213
topical grape (vitis vinifera) seed extract promotes repair of full thickness wound in rabbit.in recent years, oxidative stress and free radicals have been implicated in impaired wound healing. grape (vitis vinifera) seed extract (gse) possesses anti-inflammatory and antioxidant properties. the present study was undertaken to assess the potential activity of grape seed hydroalcoholic extract in wound healing in rabbits. rabbits of either sex were subjected to a 20 +ù 20 mm square excision made over the skin of the back. the animals were randomly divided into seven experimental groups, as ...201121816000
phenolics and antioxidant capacity of table grape (vitis vinifera l.) cultivars grown in chile.grapes (vitis vinifera l.) possess health-promoting effects attributed to their supply of a wide variety of bioactive phenolics. juice and skin fractions of 4 varieties of table grapes: red globe, crimson seedless, autumn royal, and ribier were prepared to determine and compare their total phenolics content, antioxidant capacity (dpph, frap, and orac), anthocyanins, and specific phenolics (caffeic acid, gallic acid, resveratrol, and catechin) content, since a series of positive health benefits a ...201121819404
influence of foliar fertilization with p and k on chemical constituents of grape cv. 'cardinal'.the foliar fertilization has been used as an important agrotechnical measure to avoid deficiencies and to improve quality. during the two consecutive years, a study has been performed on vitis vinifera l. (cv. 'cardinal') to examine whether a grape berry quality has been affected by the foliar application of pk fertilizer. a liquid mineral fertilizer containing 15% p2o5, 20% k2o with 0.1% b, 0.1% mn and 0.01% mo (% w/w) has been sprayed three times at rate of 8 l ha(-1) every 14-15 days starting ...201121827138
resistance to plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses.abstract:201121838877
comparison of dry, wet and microwave digestion methods for the multi element determination in some dried fruit samples by icp-oes.the aim of this study was used to investigate the level of trace metals (ba, pb, cd, mn, cr, co, ni, cu, mn, zn, sr and fe) in some dried fruits (prunus domestica l., ficus carica l., morus alba l., vitis vinifera l., prunus armeniaca l., and malus domestica) samples from turkey. trace elements were determined by icp-oes after dry, wet and microwave digestion methods in dried fruit samples. validation of the proposed method was carried out by using a nist-srm 1515-apple leaves certified referenc ...201121843582
a multidisciplinary study on the effects of phloem-limited viruses on the agronomical performance and berry quality of vitis vinifera cv. nebbiolo.viral infections are known to have a detrimental effect on grapevine yield and performance, but there is still a lack of knowledge about their effect on the quality and safety of end products. vines of vitis vinifera cv. nebbiolo clone 308, affected simultaneously by grapevine leafroll-associated virus 1 (glrav-1), grapevine virus a (gva), and rupestris stem pitting associated virus (rspav), were subjected to integrated analyses of agronomical performance, grape berry characteristics, instrument ...201121856458
varietal relationship between instrumental skin hardness and climate for grapevines (vitis vinifera l.).the main aims of this work were to classify 30 colored and white wine grape varieties according to the berry skin hardness, to assess the influence of annual variations in climate on the berry skin hardness, and to establish significant relationships among berry skin mechanical properties and some climatic-bioclimatic indices calculated for different grape-ripening periods, close to the harvest date. the results obtained show that the most influential bioclimatic indices on the skin mechanical a ...201121861480
A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity.Flavonoid pathway is spatially and temporally controlled during plant development and the transcriptional regulation of the structural genes is mostly orchestrated by a ternary protein complex that involves three classes of transcription factors (R2-R3-MYB, bHLH and WDR). In grapevine (Vitis vinifera L.), several MYB transcription factors have been identified but the interactions with their putative bHLH partners to regulate specific branches of the flavonoid pathway are still poorly understood.201121861899
Biosynthesis of the major tetrahydroxystilbenes in spruce, astringin and isorhapontin, proceeds via resveratrol and is enhanced by fungal infection.Stilbenes are dibenzyl polyphenolic compounds produced in several unrelated plant families that appear to protect against various biotic and abiotic stresses. Stilbene biosynthesis has been well described in economically important plants, such as grape (Vitis vinifera), peanut (Arachis hypogaea), and pine (Pinus species). However, very little is known about the biosynthesis and ecological role of stilbenes in spruce (Picea), an important gymnosperm tree genus in temperate and boreal forests. To ...201121865488
occurrence, sensory impact, formation, and fate of damascenone in grapes, wines, and other foods and beverages.among plant-derived odorants, damascenone is one of the most ubiquitous, sometimes occurring as an apparent natural product but more commonly occurring in processed foodstuffs and beverages. it has been widely reported as a component of alcoholic beverages, particularly of wines made from the grape vitis vinifera . although damascenone has one of the lowest ortho- and retronasal detection thresholds of any odorant, its contribution to the sensory properties of most products remains poorly unders ...201121866982
horam nonam exclamavit: sitio. the trace proteome of your daily vinegar.the trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at ph 2.2 in presence of 0.1% trifluoroacetic acid. a total of 27 unique gene products have been identified, of which 10 specific of the database vitis vinifera, 13 found in the general database uniprot_viridiplantae and 4 in swiss prot_all entries. the most abundant species detected, on the basis of spectral counts, ...201121867782
functional effect of grapevine 1-deoxy-d-xylulose 5-phosphate synthase substitution k284n on muscat flavour formation.grape berries of muscat cultivars (vitis vinifera l.) contain high levels of monoterpenols and exhibit a distinct aroma related to this composition of volatiles. a structural gene of the plastidial methyl-erythritol-phosphate (mep) pathway, 1-deoxy-d-xylulose 5-phosphate synthase (vvdxs), was recently suggested as a candidate gene for this trait, having been co-localized with a major quantitative trait locus for linalool, nerol, and geraniol concentrations in berries. in addition, a structured a ...201121868399
Analysis of high-identity segmental duplications in the grapevine genome.Segmental duplications (SDs) are blocks of genomic sequence of 1-200 kb that map to different loci in a genome and share a sequence identity > 90%. SDs show at the sequence level the same characteristics as other regions of the human genome: they contain both high-copy repeats and gene sequences. SDs play an important role in genome plasticity by creating new genes and modeling genome structure. Although data is plentiful for mammals, not much was known about the representation of SDs in plant g ...201121871077
multivariate analysis of the polyphenol composition of tempranillo and graciano red wines.vitis vinifera l. cv graciano is often used as a blending partner of tempranillo based wines because it is considered to contribute significantly to the quality. the aim of this study is to discriminate between tempranillo and graciano monovarietal wines, and those made by the incorporation of 20% of graciano variety in two different stages (at the end of malolactic fermentation and mixing the two grape varieties in the pre-fermentative maceration stage) of the winemaking process of the temprani ...201121872058
antioxidant activity of grape skin aqueous extracts from pressurized hot water extraction combined with electron paramagnetic resonance spectroscopy.pressurized hot water extraction (phwe) was employed to prepare extracts from dried grape skin of two wine grape varieties (st. laurent and alibernet) at various temperatures (from 40 up to 120°c) and amounts of sample (0.5, 1.0 and 1.5 g). to assess the antioxidant activity of the extracts, electron paramagnetic resonance (epr) spectroscopy was applied involving dpph and abts(+) assays. other extract characteristics including hplc profile of anthocyanins and total phenolic compound content were ...201121872083
novel measures of linkage disequilibrium that correct the bias due to population structure and relatedness.among the several linkage disequilibrium measures known to capture different features of the non-independence between alleles at different loci, the most commonly used for diallelic loci is the r(2) measure. in the present study, we tackled the problem of the bias of r(2) estimate, which results from the sample structure and/or the relatedness between genotyped individuals. we derived two novel linkage disequilibrium measures for diallelic loci that are both extensions of the usual r(2) measure. ...201221878986
comparison of odor-active compounds in grapes and wines from vitis vinifera and non-foxy american grape species.native american grape (vitis) species have many desirable properties for winegrape breeding, but hybrids of these non-vinifera wild grapes with vitis vinifera often have undesirable aromas. other than the foxy-smelling compounds in vitis labrusca and vitis rotundifolia , the aromas inherent to american vitis species are not well characterized. in this paper, the key odorants in wine produced from the american grape species vitis riparia and vitis cinerea were characterized in comparison to wine ...201121879766
chemical ecology and management of lobesia botrana (lepidoptera: tortricidae).the moth lobesia botrana (denis & schiffermüller) (lepidoptera: tortricidae) feeds on grapes (vitis vinifera l.), reducing yield and increasing susceptibility to fungal infections. l. botrana is among the most economically important insects in europe and has recently been found in vineyards in chile, argentina, and california. here, we review l. botrana biology and behavior in relation to its larval host (the grapevine) and its natural enemies. we also discuss current and future control strategi ...201121882674
Host suitability and gas exchange response of grapevines to potato leafhopper (Hemiptera: Cicadellidae).Although potato leafhopper, Empoasca fabae (Harris) (Hemiptera: Cicadellidae), is highly polyphagous, classic host studies do not recognize grapevines (Vitis spp.), as suitable hosts. Recently, injury has been reported and reproduction documented within grape vineyards, suggesting a host expansion for the leafhopper. To document this apparent expansion in host use, we determined whether grape plants were suitable hosts for potato leafhopper reproduction, measured the consequence of feeding injur ...201121882698
chemopreventive effects of calluna vulgaris and vitis vinifera extracts on uvb-induced skin damage in skh-1 hairless mice.solar ultraviolet radiation (uv) is a major cause of non-melanoma skin cancer in humans. photochemoprevention with natural products represents a simple but very effective strategy in the management of cutaneous neoplasia. the study investigated the protective activity of calluna vulgaris (cv) and red grape seeds (vitis vinifera l, burgund mare variety) (bm) extracts in vivo on uvb-induced deleterious effects in skh-1 mice skin. forty skh-1 mice were randomly divided into 4 groups (n=10): control ...201121893700
optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (lc-ms/ms)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (vitis vinifera l.).polyphenols in grapevine can be constitutive or induced, depending upon cultivar, plant organ, and environmental influences. the aim of the presented work was to develop and optimize a liquid chromatography-tandem mass spectrometry (lc-ms/ms) method to study the pattern and amount of selected polyphenols in leaves of vitis vinifera l. the method is simple and does not require any sample cleanup. it covers representative metabolites of the structure classes cinnamic acids, flavonoids, and stilben ...201121910493
the vitis vinifera c-repeat binding protein 4 (vvcbf4) transcriptional factor enhances freezing tolerance in wine grape.chilling and freezing can reduce significantly vine survival and fruit set in vitis vinifera wine grape. to overcome such production losses, a recently identified grapevine c-repeat binding factor (cbf) gene, vvcbf4, was overexpressed in grape vine cv. 'freedom' and found to improve freezing survival and reduced freezing-induced electrolyte leakage by up to 2 °c in non-cold-acclimated vines. in addition, overexpression of this transgene caused a reduced growth phenotype similar to that observed ...201221914113
new records of eriophyoid mites (acari: prostigmata: eriophyoidea) from saudi arabia.a field study was conducted to investigate eriophyoid mites associated with some fruit trees in riyadh, saudi arabia. the survey was carried out in four localities (el-waseel, al-beer, al-haiyer and el-deriya) and included seven species of fruit trees, namely olives (olea europea), fig (ficus carica), grapes (vitis vinifera), apple (malus domestica), citrus (citrus spp.), pomegranate (punica granatum) and pear (pyrus communis). seven new records of eriophyoid species (aceria benghalensis soliman ...201121916261
seasonal fluctuations in vitis vinifera root respiration in the field.we studied the seasonal fluctuation of soil respiration (r(s)), and its root-dependent (r(r)) and basal (r(b)) components, in a vitis vinifera (chardonnay) vineyard. the r(s) components were estimated through independent field methods (y-intercept and trenching) and modeled on the basis of a q(10) response to soil temperature, and fine and coarse root respiration coefficients. the effect of assimilate availability on r(r) was assessed through a trunk girdling treatment. the apparent q(10) for r( ...201121919911
evaluation of differences in the aroma composition of free-run and pressed neutral grape juices obtained from emir (vitis vinifera l.).in this study, the differences in the aroma compounds released after the free-run and pressed juices of cv. emir grape (vitis vinifera l.) were evaluated. aroma compounds were obtained by liquid-liquid extraction with ch(2) cl(2) , and then analyzed by gas chromatography (gc) and gas chromatography/mass spectrometry (gc/ms). according to the results, pressing uniformly increased the levels of the aromatic constituents, but this treatment lowered the grape juice quality for winemaking by increasi ...201121922666
The effects of grape seeds polyphenols on SKH-1 mice skin irradiated with multiple doses of UV-B.The study investigated the protective activity of red grape seeds (Vitis vinifera L, Burgund Mare variety) (BM) extracts in vivo on multiple doses of ultraviolet radiation (UV)-B-induced deleterious effects in SKH-1 mice skin. Eighty 8-weeks-old female SKH-1 mice were divided into 8 groups: control, vehicle, UV-B irradiated, vehicle+UV-B irradiated, BM 2.5mg polyphenols (PF)/cm(2)+UV-B irradiated, BM 4 mg PF/cm(2)+UV-B irradiated, UV-B+BM 2.5mg PF/cm(2), UV-B+BM 4 mg PF/cm(2). The extract was ap ...201121925895
enhanced extracellular production of trans-resveratrol in vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate.in this work, the effect of different inducing factors on trans-resveratrol extracellular production in monastrell grapevine suspension cultured cells is evaluated. a detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate and uv irradiation dosage, optimal cell density, elicitation time and sucrose content in the culture media. the results indicate that trans-resveratrol production decreases as the initial cell density increases for a constant elicitor concentrat ...201221927985
climate change (elevated co₂, elevated temperature and moderate drought) triggers the antioxidant enzymes' response of grapevine cv. tempranillo, avoiding oxidative damage.photosynthetic carbon fixation (a(n) ) and photosynthetic electron transport rate (etr) are affected by different environmental stress factors, such as those associated with climate change. under stress conditions, it can be generated an electron excess that cannot be consumed, which can react with o₂, producing reactive oxygen species. this work was aimed to evaluate the influence of climate change (elevated co₂, elevated temperature and moderate drought) on the antioxidant status of grapevine ...201221929631
Aqueous extract from Vitis vinifera tendrils is able to enrich keratinocyte antioxidant defences.An aqueous extract of V. vinifera L. tendrils was evaluated for its ability to enrich the antioxidant capacity of cultured cells. The long-time antioxidant capability of the extract was measured by in vitro chemical methods, and its influence on reduced glutathione levels and plasma membrane oxido reductase activity was determined in cultured human keratinocytes (NCTC 2544). Keratinocytes are cells normally exposed to oxidative stress, and for this reason adequately equipped with antioxidant def ...201121941905
Burkholderia phytofirmans strain PsJN primes Vitis vinifera L. and confers a better tolerance to low non-freezing temperatures.Several endophytic bacteria reportedly induce resistance to biotic stress and abiotic stress tolerance in several plant species. Burkholderia phytofirmans strain PsJN (PsJN) is a plant growth-promoting rhizobacterium (PGPR) that is able to colonize grapevine tissues and induce resistance to gray mold. Further, PsJN induces physiological changes that increase grapevine tolerance to low non-freezing temperatures. To better understand how bacteria induced the observed phenomena, stress-related gene ...201121942451
black fungi and associated bacterial communities in the phyllosphere of grapevine.in this study we investigate bacterial communities in association with an enriched black-fungal community in the plant phyllosphere to test whether these fungi create an environment for specific bacteria. under organic conditions of agriculture, grapevine plants (vitis vinifera) display an increased occurrence of the black fungi aureobasidium pullulans and epicoccum nigrum. their enrichment agrees with the tolerance of these fungi to copper and sulphate, both used as main fungicides in organic v ...201121944210
early leaf removal impact on volatile composition of tempranillo wines.early defoliation is a very innovative technique in viticulture used for yield management. the effects of early leaf removal performed manually and mechanically at two different phenological stages, pre-bloom and fruit set, on the volatile composition of tempranillo (vitis vinifera l.) wines were studied. volatiles were identified and quantified by gas chromatography.201221968739
characterization of red wines from south america based on sensory properties and antioxidant activity.it is widely accepted that red wines constitute one of the most important sources of dietary polyphenolic antioxidants. however, it is still not known how some variables such as variety, vintage, country of origin, and retail price are associated with the antioxidant activity and sensory profile of south american red wines. in this regard, 80 samples produced in brazil, chile and argentina were assessed in relation to their sensory properties, color and in vitro antioxidant activity, and results ...201221969030
identification of the abscisic acid receptor vvpyl1 in vitis vinifera.the phytohormone abscisic acid (aba) plays a central role in many developmental processes and in responses to several abiotic stresses. identification of the aba receptor is a first step towards understanding aba signalling. in this study, using homology analysis, we cloned three genes, named vvpyl1, vvpyl2 and vvpyl3, from vitis vinifera. an isothermal titration calorimetry assay suggested that vvpyl1 could bind to aba. a phosphatase activity assay demonstrated that vvpyl1 inhibits phosphatase ...201221974741
the péclet effect on leaf water enrichment correlates with leaf hydraulic conductance and mesophyll conductance for co(2).leaf water gets isotopically enriched through transpiration, and diffusion of enriched water through the leaf depends on transpiration flow and the effective path length (l). the aim of this work was to relate l with physiological variables likely to respond to similar processes. we studied the response to drought and vein severing of leaf lamina hydraulic conductance (k(lamina) ), mesophyll conductance for co(2) (g(m) ) and leaf water isotope enrichment in vitis vinifera l cv. grenache. we hypo ...201221988489
biological control of botrytis cinerea using the antagonistic and endophytic burkholderia cepacia cs5 for vine plantlet protection.antifungal activity of the burkholderia cepacia cs5 was tested in vitro and in vivo for the control of botrytis cinerea . bacterial biomass was significantly improved by the amendment of znso(4), mo(7)(nh(4))(6)o(24), and mannitol to the nby medium; consequently, the amount of the secreted fungicides was increased. the quantification of b. cinerea inhibition, in liquid and solid conditions, showed an important sensitivity of this fungus to the strain cs5 fungicides. microscopic monitoring impact ...201122004162
establishment and application of a metabolomics workflow for identification and profiling of volatiles from leaves of vitis vinifera by hs-spme-gc-ms.volatile organic compounds (vocs) occurring in leaves of plants carry information about the physiological state of the plant. monitoring of vocs assists in detecting plant stress before visible signs are present.201222009551
effects of introducing threatened falcons into vineyards on abundance of passeriformes and bird damage to grapes.agricultural landscapes are becoming an important focus of animal conservation, although initiatives to conserve predators to date have rarely provided economic benefits to agricultural producers. we examined whether introduction to vineyards of the new zealand falcon (falco novaeseelandiae), a species listed as threatened by the new zealand department of conservation, affected the abundance of 4 species of passeriformes that are considered vineyard pests or affected the amount of economic loss ...201222010952
polyphenol content in white table grape (vitis vinifera) berries of cultivar italia: interactive effect of irrigation, delayed harvest and storage.polyphenol concentrations were quantified by rapid resolution liquid chromatography/mass spectrometry in white table grape. the experimental vineyard was subjected to different kinds of water supply and supply rates. samples from the same vineyards were also analysed after 6 weeks of storage in a refrigerator and after 6 weeks of delayed harvesting. berry skins and seeds were analysed separately. a statistical treatment of the screening kind, namely the 2(k) full factorial design, was used for t ...201222011240
developing tools to unravel the biological secrets of rosellinia necatrix, an emergent threat to woody crops.white root rot caused by rosellinia necatrix is one of the most destructive diseases of many woody plants in the temperate regions of the world, particularly in europe and asia. recent outbreaks of r. necatrix around the globe have increased the interest in this pathogen. although the ecology of the disease has been poorly studied, recent genetic and molecular advances have opened the way for future detailed studies of this fungus.201222014332
characterization of potential aba receptors in vitis vinifera.molecular control mechanisms for abiotic stress tolerance are based on the activation and regulation of specific stress-related genes. the phytohormone abscisic acid (aba) is a key endogenous messenger in a plant's response to such stresses. a novel aba binding mechanism which plays a key role in plant cell signaling cascades has recently been uncovered. in the absence of aba, a type 2c protein phosphatase (pp2c) interacts and inhibits the kinase snrk2. binding of aba to the pyr/pyls receptors e ...201122016084
Displaying items 1201 - 1300 of 2990