Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| Impact of sugar stereochemistry on natural killer T cell stimulation by bacterial glycolipids. | Natural killer T (NKT) cells recognize glycolipids produced by Sphingomonas bacteria, and these glycolipids contain C6-oxidized sugars, either glucuronic acid or galacturonic acid, linked to ceramides. Glycolipids with gluco stereochemistry are the most prevalent. Multiple studies have demonstrated that galactosylceramides are more potent stimulators of NKT cells than their glucose isomers. To determine if this stereoselectivity is retained in the context of the C6-oxidized sugars found in bacte ... | 2011 | 21927724 |
| growth of chlorella vulgaris and associated bacteria in photobioreactors. | the aim of this study was to test three flat plate photobioreactor configurations for growth of chlorella vulgaris under non-axenic conditions and to characterize and quantify associated bacterial communities. the photobioreactor cultivations were conducted using tap water-based media to introduce background bacterial population. growth of algae was monitored over time with three independent methods. additionally, the quantity and quality of eukaryotes and bacteria were analysed using culture-in ... | 2012 | 21936882 |
| insight into the binding of the wild type and mutated alginate lyase (alyvi) with its substrate: a computational and experimental study. | the homology model of the wild type alginate lyase (alyvi) marine bacterium vibrio sp. protein, was built using the crystal structure of the family 7 alginate lyase from sphingomonas sp. a1. to rationalize the observed structure-affinity relationships of aliginate lyase alyvi with its (ggg) substrate, molecular docking, md imulations and binding free energy calculations followed by site-directed mutagenesis and alyvi activity assays were carried out. per-residue decomposition of the (ggg) bindin ... | 2011 | 21945375 |
| effects of stimulation of copper bioleaching on microbial community in vineyard soil and copper mining waste. | long-term copper application in vineyards and copper mining activities cause heavy metal pollution sites. such sites need remediation to protect soil and water quality. bioremediation of contaminated areas through bioleaching can help to remove copper ions from the contaminated soils. thus, the aim of this work was to evaluate the effects of different treatments for copper bioleaching in two diverse copper-contaminated soils (a 40-year-old vineyard and a copper mining waste) and to evaluate the ... | 2011 | 21947860 |
| role of sphingomonas sp. strain fr1 phyr-nepr-σecfg cascade in general stress response and identification of a negative regulator of phyr. | the general stress response in alphaproteobacteria was recently described to depend on the alternative sigma factor σ(ecfg), whose activity is regulated by its anti-sigma factor nepr. the response regulator phyr, in turn, regulates nepr activity in a partner-switching mechanism according to which phosphorylation of phyr triggers sequestration of nepr by the sigma factor-like effector domain of phyr. although genes encoding predicted histidine kinases can often be found associated with phyr, litt ... | 2011 | 21949070 |
| glionitrin b, a cancer invasion inhibitory diketopiperazine produced by microbial coculture. | a new diketopiperazine, glionitrin b (1), was produced using a microbial coculture of the fungus aspergillus fumigatus kmc-901 and the bacterium sphingomonas sp. kmk-001 that were isolated from acidic coal mine drainage. the structure of 1 was determined to be (3s,10as)-dithiomethylglionitrin a. this structure was determined by the analyses of extensive nmr data and the circular dichroism spectra of the natural product and a semisynthetic compound derived from glionitrin a. in contrast to glioni ... | 2011 | 21954885 |
| a rare cause of bacteremia in a pediatric patient with down syndrome: sphingomonas paucimobilis. | sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non fermentative, gram negative motile bacillus. s. paucimobilis which is widely found in nature and hospital environments rarely cause serious or life threatening infections. in this report, a case of hospital acquired bloodstream infection due to s. paucimobilis in a patient with down syndrome who was on treatment for presumed pneumonia is presented. a one year-old child patient who was a known case of down syndrome and had previously ... | 2011 | 21960744 |
| Characterization of the propanil biodegradation pathway in Sphingomonas sp. Y57 and cloning of the propanil hydrolase gene prpH. | In our previous study, the isoproturon-degrading strain Sphingomonas sp. Y57 was isolated from the wastewater treatment system of an herbicide factory. Interestingly, this strain also showed the ability to degrade propanil (3,4-dichloropropionamilide). The present work reveals that Y57 degrades propanil via the following pathway: propanil was initially hydrolyzed to 3,4-dichloroaniline (3,4-DCA) and then converted to 4,5-dichlorocatechol, which was then subjected to aromatic ring cleavage and fu ... | 2011 | 21974851 |
| identification of bottlenecks in escherichia coli engineered for the production of coq(10). | in this work, escherichia coli was engineered to produce a medically valuable cofactor, coenzyme q(10) (coq(10)), by removing the endogenous octaprenyl diphosphate synthase gene and functionally replacing it with a decaprenyl diphosphate synthase gene from sphingomonas baekryungensis. in addition, by over-expressing genes coding for rate-limiting enzymes of the aromatic pathway, biosynthesis of the coq(10) precursor para-hydroxybenzoate (phb) was increased. the production of isoprenoid precursor ... | 2011 | 21986057 |
| Enzymes involved in the biodegradation of hexachlorocyclohexane: A mini review. | The scope of this paper encompasses the following subjects: (i) aerobic and anaerobic degradation pathways of ?-hexachlorocyclohexane (HCH); (ii) important genes and enzymes involved in the metabolic pathways of ?-HCH degradation; (iii) the instrumental methods for identifying and quantifying intermediate metabolites, such as gas chromatography coupled to mass spectrometry (GC-MS) and other techniques. It can be concluded that typical anaerobic and aerobic pathways of ?-HCH are well known for a ... | 2011 | 21992990 |
| [Endogenous postpartum panophthalmitis induced by sphingomonas paucimobili]. | Endogenous panophthalmitis is a rare eye disease with purulent necrosis of all the ocular structures. It is a rare but serious condition that occurs when bacteria cross the blood-ocular barrier and multiply within the eye. Although rare, endogenous panophthamitis is a potentially devastating intraocular infection resulting in a poor visual and anatomic prognosis. We present a 39-year-old woman, without any previous history, who developed a endogenous panophthalmitis in the left eye secondary to ... | 2011 | 22003763 |
| identification of the flavin monooxygenase responsible for ipso substitution of alkyl and alkoxyphenols in sphingomonas sp. ttnp3 and sphingobium xenophagum bayram. | we previously showed that opda from sphingomonas sp. pwe1 encodes a putative flavin monooxygenase capable of transforming octylphenol (op) via type ii ipso substitution. here, we demonstrate that an opda homolog is responsible for op and related alkyl/alkoxyphenol degradation in the nonylphenol degrader sphingomonas sp. ttnp3. pcr and southern blot analyses revealed that ttnp3 contained an opda homolog, while a ttnp3 derivative unable to grow on nonylphenol (ttnp3d) did not. opda expression was ... | 2011 | 22012340 |
| bio-precipitation of uranium by two bacterial isolates recovered from extreme environments as estimated by potentiometric titration, tem and x-ray absorption spectroscopic analyses. | this work describes the mechanisms of uranium biomineralization at acidic conditions by bacillus sphaericus jg-7b and sphingomonas sp. s15-s1 both recovered from extreme environments. the u-bacterial interaction experiments were performed at low ph values (2.0-4.5) where the uranium aqueous speciation is dominated by highly mobile uranyl ions. x-ray absorption spectroscopy (xas) showed that the cells of the studied strains precipitated uranium at ph 3.0 and 4.5 as a uranium phosphate mineral pha ... | 2011 | 22019055 |
| compositions of microbial communities associated with oil and water in a mesothermic oil field. | samples of produced water and oil obtained from the enermark field (near medicine hat, alberta, canada) were separated into oil and aqueous phases first gravitationally and then through centrifugation at 20°c in an atmosphere of 90% n(2) and 10% co(2). biomass that remained associated with oil after gravitational separation (1×g) was dislodged by centrifugation at 25,000×g. dna was isolated from the aqueous and oil-associated biomass fractions and subjected to polymerase chain reaction amplifica ... | 2011 | 22038128 |
| the effect of sterile versus non-sterile tourniquets on microbiological colonisation in lower limb surgery. | surgical tourniquets are commonplace in lower limb surgery. several studies have shown that tourniquets can be a potential source of microbial contamination but have not compared the use of sterile versus non-sterile tourniquets in the same procedures. | 2011 | 22041233 |
| optimization of fenton process for decoloration and cod removal in tobacco wastewater and toxicological evaluation of the effluent. | decoloration and chemical oxygen demand (cod) removal in tobacco wastewater by fenton process has been optimized under 25 +/- 2 degrees c. the results showed that the optimal range of conditions were ph 4.13-4.66, fe(2+) 0.29-0.34 g/l and h2o2 > or = 2.73 g/l. within this range, up to 95% of colour and 90% of cod was removed. in an enlarged system, setting the optimal conditions as ph 4.50, fe(2+) 0.34 g/l and h2o2 4.00 g/l, the colour removal rate was 96.03 +/- 2.57%, with cod removal rate of 9 ... | 2011 | 22049737 |
| sphingomonas paucimobilis bacteremia related to intravenous human immunoglobulin injections. | 2011 | 22075255 | |
| phyllosphere bacterial communities of trichome-bearing and trichomeless arabidopsis thaliana leaves. | this study aimed to investigate whether the presence of trichomes as conspicuous physical attributes of the leaf surface affects the microbial community composition on arabidopsis thaliana leaves. the a. thaliana ecotype col-0 and its trichomeless gl1 mutant were grown in growth cabinets under climate-controlled conditions. the gl1 mutant showed a similar wax composition as the col-0 wild type with slightly reduced amounts of c(29), c(31) and c(33) alkanes by gc/ms and gc/fid analyses. 120 bacte ... | 2011 | 22080429 |
| culturable bacterial communities on leaf sheaths and panicles of rice plants in japan. | culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ... | 2011 | 22081407 |
| parasphingopyxis lamellibrachiae gen. nov., sp. nov., isolated from a marine organism in kagoshima bay, japan. | a gram-negative, aerobic, motile, orange-pigmented, slightly halophilic, rod-shaped bacterium, designated strain jamh 0132t, was isolated from a tubeworm trophosome in kagoshima bay, japan, and subjected to polyphasic taxonomic characterization. the isolate grew optimally at 28-30 °c and in the presence of 2-3 % nacl. chemotaxonomic analysis showed that q-10 was the predominant respiratory quinone and that c18:1 ω7c, c16:0 2-oh and c16:0 were the major fatty acids. sphingoglycolipids, phosphatid ... | 2011 | 22081711 |
| transcriptome and membrane fatty acid analyses reveal different strategies for responding to permeating and non-permeating solutes in the bacterium sphingomonas wittichii. | sphingomonas wittichii strain rw1 can completely oxidize dibenzo-p-dioxins and dibenzofurans, which are persistent contaminants of soils and sediments. for successful application in soil bioremediation systems, strain rw1 must cope with fluctuations in water availability, or water potential. thus far, however, little is known about the adaptive strategies used by sphingomonas bacteria to respond to changes in water potential. to improve our understanding, strain rw1 was perturbed with either the ... | 2011 | 22082453 |
| characterization of microcystin-lr removal process in the presence of probiotic bacteria. | toxic cyanobacteria have been reported in lakes and reservoirs in several countries. the presence of toxins in drinking water creates a potential risk of toxin transference for water consumers. besides chemical and physical methods of cyanotoxin removal from water, biodegradation methods would be useful. the aim of the current study was to identify bacterial removal mechanisms of the hepatotoxin microcystin-lr. this was studied by testing the hypothesis of enzymatic degradation of microcystin-lr ... | 2011 | 22115989 |
| genome sequence of sphingomonas elodea atcc 31461, a highly productive industrial strain of gellan gum. | the commercial gelling agent gellan gum is a heteropolysaccharide produced by sphingomonas elodea atcc 31461. however, the genes involved in the biosynthesis, regulation, and modification of gellan gum have not been fully characterized. here we describe the draft genome sequence of stain atcc 31461 and major findings from its annotation. | 2011 | 22123766 |
| molecular phylogeny of the genus dactylopius (hemiptera: dactylopiidae) and identification of the symbiotic bacteria. | phylogenetic analyses, from polymerase chain reaction (pcr)-amplified 12s rrna and 18s rrna gene sequences from cochineal insects of the genus dactylopius present in mexico, showed that d. ceylonicus, d. confusus, and d. opuntiae are closely related. d. coccus constitutes a separate clade, and d. tomentosus is the most distantly related. bacterial 16s rrna sequences from all the dactylopius species sampled showed a common ß-proteobacteria, related to azoarcus, also found in eggs and in bacterio ... | 2010 | 22127169 |
| Molecular identification of a polyM-specific alginate lyase from Pseudomonas sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate. | An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingomonas sp. strain A1. The proposed 3-D structure of KS-408 alginate lyase shows that Asn-198, His-199, Arg-246, and Tyr-253 residues are conserved for the catalytic active site. T ... | 2011 | 22145710 |
| Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria. | The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ... | 2011 | 22163022 |
| Characterization of the first step involved in enzymatic pathway for microcystin-RR biodegraded by Sphingopyxis sp. USTB-05. | Enzymes encoded by genes biodegrading microcystins (MCs) can help reveal the function of genes and biodegradation pathway of MCs. Here the first and important gene (USTB-05-A, 1,008bp) involved in biodegradation of microcystin-RR (MC-RR) was cloned from Sphingopyxis sp. USTB-05 and firstly expressed in Escherichia coli BL21 (DE3) with an expression vector of pGEX4T-1 successfully. The nucleotide sequences of cloned USTB-05-A possessed 92.5% homology to that of mlA reported in Sphingomonas sp. st ... | 2011 | 22177937 |
| food-borne bacteremic illnesses in febrile neutropenic children. | bacteremia following febrile neutropenia is a serious complication in children with malignancies. preventive measures are currently targeted at antimicrobial prophylaxis, amelioration of drug-induced neutropenia, and nosocomial spread of pathogens, with little attention to community-acquired infections. a retrospective study was conducted at a pediatric oncology center during a 3-year period to identify probable cases of food-borne infections with bacteremia. twenty-one bacteremic illnesses affe ... | 2011 | 22184532 |
| biodiversity characterization of cellulolytic bacteria present on native chaco soil by comparison of ribosomal rna genes. | sequence analysis of the 16s ribosomal rna gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which actinobacteria, proteobacteria and acidobacteria accounted for more than 76% of all sequences. in both enriched samples, members of proteobacteria were the most frequently represented. the majority of ... | 2011 | 22202170 |
| bacterial communities in an ultrapure water containing storage tank of a power plant. | ultrapure waters (upws) containing low levels of organic and inorganic compounds provide extreme environment. on contrary to that microbes occur in such waters and form biofilms on surfaces, thus may induce corrosion processes in many industrial applications. in our study, refined saltless water (upw) produced for the boiler of a hungarian power plant was examined before and after storage (sampling the inlet [tke] and outlet [tku] waters of a storage tank) with cultivation and culture independen ... | 2011 | 22207294 |
| growth and identification of bacteria in n-halamine dental unit waterline tubing using an ultrapure water source. | this study examined bacterial growth and type on biofilm-controlling dental unit waterline (duwl) tubing (t) and control manufacturer's tubing (c) in a laboratory duwl model using ultrapure source water that was cycled through the lines. sections of tubing lines were detached and examined for biofilm growth using sem imaging at six sampling periods. bacteria from inside surfaces of t and c, source unit, and reservoir were cultured and enumerated. at six months, organisms were molecularly identif ... | 2011 | 22220171 |
| coenzyme q(10) production by immobilized sphingomonas sp. zute03 via a conversion-extraction coupled process in a three-phase fluidized bed reactor. | a three-phase fluidized bed reactor (tpfbr) was designed to evaluate the potential of coq(10) production by gel-entrapped sphingomonas sp. zute03 via a conversion-extract coupled process. in the reactor, the coq(10) yield reached 46.99mg/l after 8h of conversion; a high-level yield of about 45mg/l was maintained even after 15 repetitions (8h/batch). to fully utilize the residual precursor (para-hydroxybenzoic acid, phb) in the aqueous phase, the organic phase was replaced with new solution conta ... | 2012 | 22226200 |
| sphingomonas laterariae lnb2t sp. nov. isolated from hexachlorocyclohexane (hch) contaminated dumpsite in lucknow. | a gram-negative, non-motile, cream coloured and rod shaped bacterial strain named lnb2t was isolated from a brick kiln area situated in an open hexachlorocyclohexane (hch) dumpsite at ummari village (lucknow, india) and was subjected to polyphasic taxonomic approach. strain lnb2t showed 16s rrna gene sequence similarity of 98.0 % with its closest relative sphingomonas haloaromaticamans a175t and 97.3 % with sphingomonas histidinilytica um2t. strain lnb2t formed a monophyletic group in a cluster ... | 2012 | 22247216 |
| novel low-temperature-active, salt-tolerant and proteases-resistant endo-1,4-β-mannanase from a new sphingomonas strain. | sphingomonas sp. jb13, isolated from slag of a >20-year-old phosphate rock-stacking site, showed the highest 16s rdna (1343bp) identity of 97.2% with sphingomonas sp. erb1-3 (fj948169) and <97% identities with other identified sphingomonas strains. a mannanase-coding gene (1191bp) was cloned and encodes a 396-residue polypeptide (manajb13) showing the highest amino acid sequence identities of 56.2% with the putative glycosyl hydrolase (gh) family 26 endo-1,4-β-mannanase from rhodothermus marinus ... | 2012 | 22265897 |