Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| age related changes in serum throxine and 125i-triiodothyronine resin sponge uptake in the young dog. | serum thyroxine (t4) concentrations and 125i-labelled triiodothyronine resin sponge uptakes (t3-rsu) were determined in young dogs every 10 days between 3 and 103 days of age. when compared to the adult mean of 1.7 microgram t4/100 ml serum, the t4 in young dogs was elevated throughout the ages studied. the serum t4 concentration was twice the adult value at 3 days of age, rose to over five times the adult value in the 1st month of life, and declined again to twice the adult mean by 103 days of ... | 1977 | 592719 |
| the radioimmunological measurement of thyroglobulin secretion in vitro under the influence of some herbicides. | a radioimmunoassay (ria) technique of human thyroglobulin (tgl) was developed using 125i-tgl and a rabbit anti-tgl serum at the initial dilution of 1:100,000. the sensitivity of the technique was of 2.5 ng/ml. the ria of tgl was applied as a screening test for the in vitro secretory activity of human thyroid cells under the influence of two herbicides of the heterocyclic group (amitrol and atrazin). three levels of concentration of amitrol and atrazin were administered to the tissue culture tube ... | 1977 | 594626 |
| [cryosurgical treatment of prostate gland cancer]. | the authors share their experience with cryosurgical treatment of 38 patients with prostatic cancer (stage t3--in 9 patients, t4--in 29). the technic of cryotherapy is described. the former was employed in patients with complete obstruction of the cervico-urethral segment being nonresponsive to the hormone- and radiotherapy, and also for a life cystostome. three freezing sessions for 5--15 minutes were conducted with natural warming of the prostate between sessions during 10--12 minutes, and for ... | 1977 | 595515 |
| the effect of body temperature on thyroid hormone levels in patients with non-thyroidal illness. | during studies on the mechanism underlying the low serum t3 levels in euthyroid patients with various acute and chronic non-thyroidal illnesses, it became evident that body temperature may be one parameter associated with changes in serum t3 levels. forty-nine hospitalized, euthyroid patients with hyperpyrexia caused by various non-thyroidal illnesses were studied. the levels of serum t3 were found to decrease gradually with increasing body temperature. t3 was already below the normal level +/- ... | 1977 | 596246 |
| thyroid hormone kinetics: improved method for quantitative separation and measurement of the various radioiodothyronine injection. | an accurate and reproducible method for measurement of radioactive species in blood after in vivo injection of labelled iodothyronines is described. by extraction with high-affinity antisera, radioactive reverse t3 and t3 are separated from serum quantitatively. radioiodide is quantitatively separated from radio-thyronine species and serum proteins by sephadex g50 filtration. the residual mixture of radio-t4 and iodoprotein is quantitatively resolved by ion-exchange adsorption. minimal misclassi ... | 1977 | 598013 |
| joining of synthetic ribotrinucleotides with defined sequences catalyzed by t4 rna ligase. | rna ligase catalyzed the joining of pc-c-ap with c-a-a in the synthesis of c-a-a-c-c-ap, which has the sequence of the escherichia coli trnafmet 3'-end. pc-c-a was also shown to be joined to c-a-a without any undesired self-polymerization. joining of pc-c-a to various synthetic ribotriplets, such as c-c-a, a-a-a, c-c-c, u-u-u, u-a-g, c-c-g and u-u-c, was performed as well as joining to the partially substituted trimers with a photolabile o-nitrobenzyl group, c-anbzl-a and c-c-anbzl. the yields w ... | 1977 | 598370 |
| effect of insulin on thyroid hormone blood levels in normal and thyroidectomized dogs. | administration of insulin brings about an increase of thyroid hormone blood concentration. this study investigated the possibilities that the elevation is due to increased thyroid secretion or to liberation of hormone from hepatic stores. monocomponent insulin was administered to intact dogs by jugular perfusion (25 mu/kg/min. for 15 min.) and to thyroidectomized dogs either as single dose i.m. (1 u/kg) or by jugular perfusion (as above). all animals showed at least halving of blood glucose conc ... | 1977 | 598564 |
| action of t4 endonuclease v on dna containing various photoproducts. | the action of t4 endonuclease v on dna containing various photoproducts was investigated. (1) the enzyme introduced strand breaks in dna from ultraviolet-irradiated vegetative cells of bacillus subtilis but not in dna from irradiated spores of the same organism. dna irradiated with long wavelength (360 nm peak) ultraviolet light in the presence of 4,5',8-trimethylpsoralen was not attacked by the enzyme. these results indicate that 5-thyminyl 5,6-dihydrothymine (spore photoproduct) and psoralen m ... | 1977 | 599143 |
| a mutation which bypasses the requirement for p24 in bacteriophage t4 capsid morphogenesis. | 1977 | 599558 | |
| studies related to the head-maturation pathway of bacteriophages t4 and t2:i. morphology and kinetics of intracellular particles produced by mutants in the maturation genes. | mutants in the genes governing the maturation of the head of bacteriophage t4 and in gene 24 were studied by electron microscopy of thin sections. we define morphologically: black particles, comprising mature, stable heads and immature, fragile heads, which break down upon lysis; grizzled particles, which apparently are partially filled or partially emptied; empty large particles without dna or core which are all the same size as normal heads; empty small particles without dna and without core w ... | 1977 | 599933 |
| studies related to the head-maturation pathway of bacteriophages t4 and t2:ii. nuclear disruption, protein synthesis and particle formation with the mutant 43-.30-.46-. | we describe the aberrant phage multiplication of the triple conditional lethal mutant 43-(polymerase).30-(ligase).46-(exonuclease) of bacteriophage t4d in which phage dna replication is arrested but some late protein synthesis occurs (33). the nuclear disruption is indistinguishable from wild type. forty-five empty small and empty large particles are assembled per cell when the multiplicity of infection (m.o.i.) is 100. this number corresponds closely to the 38 phage equivalents of cleaved major ... | 1977 | 599934 |
| molecular cloning of fragments of bacteriophage t4 dna. | non-glucosylated t4 dna was digested with r.ecori and the resulting fragments covalently joined to lambda vectors. the genetic content of each lambda-t4 hybrid was determined by marker-rescue tests. the isolation of many recombinants containing partial-digestion products of t4 dna provided the overlapping sequences necessary to order fragments within the t4 genome. the present analyses include parts of the "early" region between genes 42 and 46, and much of the "late" region between genes 50 and ... | 1977 | 600265 |
| radioimmunoassay of thyroxine and triiodothyronine in urine using extraction and separation of sephadex columns. | radioimmunological methods for the determination of thyroxine (t4) and triiodothyronine (t3) in urine have been developed. the methods are based on trapping of t4 and t3 from unextracted urine, followed by separation between free and antibody bound hormone on the same sephadex column. the t4 method has been compared to a competitive protein binding (cpb) assay using ethyl acetate extraction. the methods are evaluated in sixty-seven euthyroid controls, twenty-four hyperthyroid and seven hypothyro ... | 1977 | 601507 |
| radioimmunological determination of reverse triiodothyronine in unextracted serum and serum dialysates. | a sensitive radioimmunoassay for measurements of 3,3'-5'-triiodothyronine (reverse t3, rt3) in small amounts of unextracted serum is described. the interference of rt3 binding proteins in serum was precluded by addition of 8-anilino-1-naphthalene sulphonic acid (ans). the cross reaction of t4 with the rt3 anti-serum varied with the concentration of t4 in the samples. at 50 per cent inhibition of [125i]rt3 binding, the t4 cross reaction was 0.075% (mol/mol). all values were corrected for t4 cross ... | 1977 | 601517 |
| [demonstration of an agglutination-like nature of haptoglobins against streptococci with t4-antigen through the examination of umbilical cord sera]. | the determinability of haptoglobin types is correlated with the titers of agglutination against streptococci carrying the t4 antigen. if the haptoglobin types in cord-sera cannot be determined (as a result of the physiological binding of haptoglobins) the agglutination reaction fails or the titers are greatly diminished. the dimorphism, which was found in sera of adults, can be demonstrated in cord sera, too, if they can be typed. it is concluded that the observed reaction between hp 2-2 and hp ... | 1977 | 602509 |
| procedures for characterisation of the genetic material of candidate vaccine strains. | two procedures for characterising the genomes of recombinant influenza viruses are described. the first of these involves ribonuclease t4 oligonucleotide fingerpart analysis of separated viral rnas labelled either in vivo or in vitro and the second utilises polyacrylamide gel electrophoresis to identify the double-stranded molecules formed by hybridisation between the complementary and virion rnas of two viruses. although the latter method is more suitable for routine screening purposes, both pr ... | 1977 | 604097 |
| serum hormones in the perinatal pig and the effect of exogenous insulin on blood sugars. | growth hormone (gh), insulin, cortisol, triiodothyronine (t3) and thyroxine (t4) were determined in serum of fetal pigs at 90, 100, 110 and 114 days of gestation and of newborn pigs at 1 and 24 hours of age. fetal gh, t3 and cortisol increased between 90 and 110 days. gh concentration decreased between 110 and 114 days, while t3 and cortisol were unchanged. t4 was variable in fetal pigs and no relationship with t3 levels was apparent. fetal insulin was constant at approximately 10 muu/ml. in new ... | 1977 | 604183 |
| evaluation of thyroid functions in leprosy. i. thyroid function tests. | twenty six patients of different types of leprosy were studied for radio active iodine uptake (i131) and serum levels of triiodothyronine (t3), thyroxine (t4) and thyroid stimulating hormone (tsh). none of the patients had clinical evidence of thyroid involvement. no significant difference was found between the values obtained in patients and normals and in different varieties of leprosy. | 1977 | 604656 |
| [clinical prognostic factors in breast cancer]. | five year survival of 683 mammary carcinomas would confirm the prognostic value of t.n.m. classification (uicc), of tumor particular characteristics (size, fixation to neighbouring structures) and presence or absence of clinical nodes. a clinical-histological relationship was studied for axillary nodes: in our experience, clinical determination is more reliable for estimation of n0 and n2 than n1. three groups of age were considered; the age, t and n corrected five year survival show a higher mo ... | 1977 | 607998 |
| acute decrease of blood thyroid hormone by isovolemic exchange transfusion as a stimulus for pulse tsh release and its modifications by cns lesions. | the level of thyroid hormone in blood has been acutely decreased by about 20--30% within 20 min with the aid of isovolemic exchange transfusion (iet) of thyroid hormone free blood cell suspension (thfbcs) in three groups of rats: (1) intact control (c); (2) median eminence (me)-lesioned; (3) thalamus (th)-lesioned. the levels of thyroxine (t4) and tsh were measured by specific radioimmunoassay (ria) before the transfusion and for 180 min after that. the level of t4 increased to the initial value ... | 1977 | 609370 |
| [role of thymine dimers in the uv mutagenesis of bacteriophage sd]. | the sensitized irradiation (lambda greater than 310 nm) with acphim induces plaque mutants of extracellular phage sd. thus, tt in phage sd dna are both lethal and mutagenic. the yield of plaque mutants is about 3-fold lower as compared with the yield after uv-irradiation (lambda = 254 nm). the theoretical analysis of uv-induced mutagenesis curves of phage sd was carried out. it is concluded that the following photoproducts make a contribution in the mutagenic effect of near-uv (lambda = 254 nm): ... | 1977 | 612486 |
| some changes in the composition of blood during the first 24 hours after birth in normal and growth retarded lambs. | changes in the composition of blood which occurred during the first 24 hr after birth were examined in 18 lambs whose prenatal growth had been reduced by limiting the size of the placenta (group ce) and in 18 lambs born to untreated ewes (group r). the gestational period was about 4.5 days shorter in group ce than in group r and the birth-weights of group ce lambs were reduced by about 0.5 kg. at birth the pcv and the plasma concentrations of glucose were significantly higher and the plasma conc ... | 1977 | 615517 |
| primary humoral immune response against tumor membrane antigens and foreign antigens by plasma cell tumor bearer mice. | the mopc-460 plasma cell tumor possesses tumor specific antigens. it nevertheless grows and kills its syngeneic host. the possible impairment of immune responsiveness in tumor bearing mice was investigated by measuring their ability to mount a humoral immune response against foreign antigens, such as sheep red blood cells or bacteriophage t4. no significant decrease in the response to either antigen was found until the tumor mass exceeded 10-15% of the host's body weight. moreover, circulating a ... | 1977 | 615743 |
| a critical analysis of some thyroid function tests. | the results of a comparative study of thyroid function tests are reported, the assay for pbi having been used for reference. the merits and sources of error of the bio-rad column test, thyopac-4 test and t3-ria test are discussed with reference to pbi. the correlation coefficients and the sources of error being taken into consideration, pbi represents a fairly reliable indicator of t4 values and recommends itself on these grounds as a basic routine procedure, the more so as it is simple, cheap a ... | 1977 | 617927 |
| early detection of neonatal hypothyroidism by serial tsh determination in dried blood. six months experience with a reliable, efficient and inexpensive method. | mass newborn screening for primary hypothyroidism was introduced in switzerland on january 1st, 1977, using a radioimmunoassay of tsh in dried blood spotted on filter paper. after incubation for 38 h at 20 degrees c, bound and free tsh is separated by double antibody precipitation. the filter paper discs of 6.5 mm diameter remain in the test tubes. at present, one tsh determination costs approx. sfr. 4.40. all reagents used are commercially available and their costs amount to not more than 15% o ... | 1977 | 617976 |
| metabolism of thyroid hormones during aging. | the paper presents experimental data on extrathyroidal metabolism of thyroid hormones in rats of various ages (1.5--32 months):: t4-131i distribution in an organism, indices of t4 and t3 kinetics, tissue deiodinative capacity, formation of t4 tissue metabolism products and their clearance from cells and organism, t4 binding with tissue proteins. essential changes in t4 peripheral tissue metabolism have been found with aging: t4 binding by tissues is decreased; t4 distribution space and metabolic ... | 1978 | 618772 |
| dose-response studies on the inhibitory effect of thyroid hormones on insulin secretion in the rat. | dose-response studies have been performed to investigate the effect of thyroid hormones on insulin secretion from the rat pancreas with special reference to the time course of the hormone effect, doses of triiodothyronine (t3) and thyroxine (t4), and glucose concentration in the perfusion medium. the prominent effect of thyroid hormones was the inhibition of the late phase of glucose-induced insulin secretion. as the late phase comprises at least 98%-99% of insulin released from the pancreas dur ... | 1978 | 619228 |
| thyroid function in lung cancer. | thyroid function was assessed at the time of initial diagnosis in 204 patients with lung cancer and compared with that of age and sex-matched patients with non-malignant lung disease. abnormalities in thyroid function were found in 67 patients (33%). the most prevalent abnormality was a low t3 concentration; this was not associated with other clinical or biochemical evidence of hypothyroidism, but the short-term prognosis of these patients was worse than that of matched patients with lung cancer ... | 1978 | 620266 |
| nuclear binding of triiodothyronine and thyroxine in lymphocytes from subjects with hyperthyroidism, hypothyroidism and resistance to thyroid hormones. | binding of triiodothyronine (t3) and thyroxine (t4) to nuclei of intact human lymphocytes was studied. the binding characteristics were analysed by scatchard's method. in lymphocytes from euthyroid healthy subjects there was a single set of saturable nuclear t3 and t4 binding sites with an apparent mean equilibrium association constant of 3.3 x 10(10) l/mol and 1.7 x 10(10) l/mol, respectively. the estimated mean maximal specific binding capacity for t3 was 50 fmol/mg dna and for t4 was 55 fmol/ ... | 1978 | 620467 |
| modification of a screening program for neonatal hypothyroidism. | from our experience in the screening of 212,000 newborn infants, we have devised a flow chart for processing t4 and tsh measurements obtained from initial filter paper blood spots. to date, all infants with thyroid dysfunction or tbg deficiency have been detected. of the population screened, 1.84% require a spot tsh determination, and 1.1% require repeat determinations of t4. | 1978 | 621609 |
| revised location of the riii gene on the genetic map of bacteriophage t4. | two- and three-factor crosses showed that the t4 riii gene is located between genes 31 and 30 rather than between genes 31 and 63. | 1978 | 621784 |
| mutator and antimutator phenotypes of suppressed amber mutants in genes 32, 41, 44, 45, and 62 in bacteriophage t4. | bacteriophage t4 genes 32, 41, 44, 45, 56, and 62 are essential to dna replication. amber mutants (suppressed by su+1, su+2, or su+3 bacteria) in these genes were examined for any mutator or antimutator effects on the reversion of a transition mutation. in every case except for mutations in gene 56, elevated or lowered error frequencies were observed. these results indicate the importance of all of the replicative proteins in the determination of error frequency. | 1978 | 621787 |
| enhancement of fetal lung maturity by intra-amniotic administration of thyroid hormone. | following preliminary results in animal studies demonstrating that thyroxine (t4) injected directly into the fetus or the amniotic sac is capable of enhancing fetal lung maturity, a trial of thyroxine treatment in mean was carried out. two hundred micrograms of t4 were injected intra-amniotically in eight women with high-risk pregnancies in which premature delivery was inevitable or indicated. fetal lung maturity was evaluated by a recently developed, accurate, and rapid method using the microvi ... | 1978 | 623168 |
| increased urinary excretion of triiodothyronine (t3) and thyroxine (t4) and decreased serum thyreotropic hormone (tsh) induced by motion sickness. | we exposed 35 male subjects to a rotary chair and motion sickness was provoked by coriolis effect. this stress caused an increased excretion of urinary t3 and t4 and a decrease of tsh levels in serum. the increment in urinary excretion of thyroid hormones may serve as a very useful measure for the quantitation of physical stress. although no statistically significant change of t3, t4, and tbg levels in serum could be observed by the employed techniques, the hypothesis is favoured that motion sic ... | 1978 | 623566 |
| t4 polynucleotide ligase catalyzed joining of short synthetic dna duplexes at base-paired ends. | the self-complementary octanucleotide dt-a-g-t-a-c-t-a has been synthesized and its sequence confirmed by two-dimensional fingerprinting. under conditions used for the t4 polynucleotide ligase reaction, this oligonucleotide forms a dimeric duplex which shows a tm of 18 degrees c. the optimal rate of joining of the 32p-labeled duplex occurs between 12 and 15 degrees c. the rate is highly concentration dependent, as expected for a bimolecular process. polyacrylamide gel electrophoretic analysis of ... | 1978 | 623741 |
| interaction of normal and unusually modified microbial dna with cultured mammalian cells. breakdown and reincorporation vs. uptake of polymerized dna. | the uptake of radioactively labeled bacterial and phage dna and the incorporation of acid-soluble dnaase i digests of these dnas by cultures of human foreskin and 3t3 cells were studied. the presence of large amounts of unusually modified pyrimidine residues in donor phage dnas allowed radioactive donor dna in the nuclei of dna-treated cells to be distinguished from host dna labeled with breakdown products derived from donor dna. this distinction could be made because it was found that radioacti ... | 1978 | 623763 |
| thyroid hormone concentrations in a large scale community survey. effect of age, sex, illness and medication. | total thyroid hormone concentrations have been measured in the course of a large scale community survey to determine the distribution of these variables in the normal population and to assess the effect of age, sex, previously undectected thyroid disease and medication upon these parameters. 2779 subjects were studied. serum t4 concentrations were normally distributed. a progressive increase in t4 levels with age was noted in the males, and a smaller increase in females which was concealed by th ... | 1978 | 624178 |
| distant metastases in nasopharyngeal carcinoma. | three hundred and fifty-two consecutive cases of nasopharyngeal carcinoma were analysed to determine the pattern of distant metastases. diagnosis of distant metastases was made wholly on clinical signs and radiography. ninety-nine patients developed distant metastases, an incidence rate of 28.1%. there did not appear to be any difference in "risk" by age or sex. the predilection of this cancer to develop skeletal metastases is further confirmed. pulmonary and hepatic involvement were the next co ... | 1978 | 624198 |
| thyroid function tests in elderly hyperthyroid patients. | several tests of thyroid function were performed in 35 hyperthyroid patients over the age of 65 (elderly). the results were compared to those of similar tests in 48 hyperthyroid patients under the age of 65 (young). total serum thyroxine (t4) was within the normal range in 14 percent of the elderly and 11 percent of the young hyperthyroid patients. the free thyroxine index (fti) was within the normal range in 11 percent of both groups. the triiodothyronine uptake (t3u) proved to be a poor test i ... | 1978 | 624818 |
| structure of the lysozyme from bacteriophage t4: an electron density map at 2.4 a resolution. | 1978 | 625058 | |
| neural and endocrine development after chronic tryptophan deficiency in rats: ii. pituitary-thyroid axis. | long-evans female rats, 21 days of age, were weaned and placed on a control (purina rat chow) diet, on a tryptophan deficient diet (t-) and on a diet complete in quality (purina rat chow) but restricted to the daily amount consumed by the rats on the t- diet (pair-feeding). all animals were maintained on these diets for 1 and 2 months and then one group of t- rats was returned to the complete (purina) diet and kept on this diet for several periods of time (up to 2 months). growth was interrupted ... | 1978 | 625148 |
| [contritution of correction to genetic recombination in t4 phage measured by the effect of map contraction]. | marker-dependence of the fine structure map contraction in t4 phage is studied in two-factor crosses between riib mutants separated by indicator distances. the genetic intervals, which were short as compared with mean length of the heteroduplex region in hybrid dna molecules but which exceeded the length of the dna strand involved in a single correction event, were selected as indicator ones. on the basis of a deviation of measured frequencies from additivity (map contraction) the marker-specif ... | 1978 | 627341 |
| the usefulness of pretreatment dncb in 85 patients with squamous cell carcinoma of the upper aerodigestive tract. | pretreatment dncb skin testing and absolute lymphocyte counts were studied in 85 consecutive head and neck cancer patients. all patients were treated primarily with radiation therapy alone or combined radiation plus surgery. the results of dncb testing and absolute lymphocyte counts were not sufficiently predictive of outcome, (no evidence of disease after 2 years) to be useful in making treatment decisions on an individual patient. this applied to the good prognosis (t1, t2, n0-n3) and poor pro ... | 1978 | 628221 |
| [analysis of the neuronal activity of the lateral geniculate body in cats]. | activity of the lateral geniculate body neurons was studied in immobilized cats. four parameters of impulse activity of every neuron were determined by computer analysis: t1, minimal, t2, average interspike intervals of the spontaneous activity, t3, latent period, and t4, duration of the first cluster of poststimulation activity. classification of neuronal groups was made according to these parameters. this classification was used as a basis for the construction of a scheme of neuronal interacti ... | 1978 | 628469 |
| pyrazole-induced thyroid necrosis: a distinct organ lesion. | one oral dose of pyrazole caused necrosis of rat thyroid follicular epithelial cells but spared the parafollicular (c) cells and the parathyroid glands. serum thyroxine (t4) and triiodothyronine (t3) were significantly decreased on day 3 after pyrazole administration and were immeasurable on day 5. at day 5 the thyroid was enlarged and the concentration of thyroid-stimulating hormone in the serum was increased, indicating an appropriate pituitary response to a primary lesion in the thyroid. dose ... | 1978 | 628835 |
| why does anyone still use desiccated thyroid usp? | the effect on thyroid status of changing from thyroid usp to sodium l-thyroxine was evaluated in 40 patients. with thyroid, abnormally high triiodothyronine (t3) levels were seen in 36 of 38 patients receiving doses of 90 to 240 mg; compared to sodium l-thyroxine, 0.15 to 0.2 mg, the serum t3 was higher (289 +/- 15 ng/dl versus 176 +/- 9 ng/dl, p less than 0.0005) and the thyroxine (t4) lower (7.4 +/- 0.3 microgram/dl versus 11.6 +/- 0.5 microgram/dl, p less than 0.01). thyrotoxic symptoms occur ... | 1978 | 629277 |
| [serum t4 determination with radioimmunoassay--studies of tetra-tab ria kit]. | 1978 | 630732 | |
| [bacteriophage t4 as a model for the study of cooperative movement processes]. | 1978 | 631007 | |
| reciprocal changes in serum free thyroxine fraction and thyrotropin level after administration of thiocyanate to rats. | the administration of thiocyanate to rats caused a significant increase of serum free thyroxine fraction, which coincided with the significant decrease of tsh level. the other components (aft4, t4, t3) in serum at this time were decreased or unchanged. the finding suggests the role of free thyroxine fraction in feed-back regulation of tsh secretion. | 1978 | 631286 |
| [a radioimmunoassay for the measurement of thyroglobulin in human serum and its application to clinical study (author's transl)]. | a sensitive and specific double antibody radioimmunoassay for the measurement of serum thyroglobulin (tg) has been developed. the minimum detectable concentration of tg was 5.0 ng/ml. coefficients of within and between assay variation were 2.4% and 12.0%, respectively. the mean recovery of tg added to normal serum was 102.9%; and t4, t3, dit and mit did not crossreact in this assay system. dilution curves of normal thyroid extract, tumor extract and patient's serum were shown to be parallel with ... | 1978 | 631380 |
| effect of fasting on t3 and t4 concentrations in chicken serum. | 1978 | 631553 | |
| structural changes in the t4 gene 32 protein induced by dna polynucleotides. | alterations in the structure of the dna-binding protein specified by gene 32 of bacteriophage t4 have been detected using partial trypsin digestion as a conformational probe. limited tryptic hydrolysis of the gene 32 protein removes a fragment ("b" region), of 21 amino acids from the nh2 terminus and a 6,200-dalton fragment ("a" region) from the cooh terminus. poly(dt), poly(dc), and single-stranded dna increase the rate of tryptic hydrolysis of the "a" region but decrease the rate of tryptic hy ... | 1978 | 632279 |
| serum thyroid hormone concentrations during prolonged reduction of dietary intake. | in nine obese but otherwise healthy subjects the effect of caloric restriction on the serum concentrations of thyroxine (t4), 3,3',5-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), urea, uric acid, creatinine, and bilirubin was studied. blood was obtained before and 2, 4, and 6 wk after the subjects had changed to a chemically defined diet (31 g amino acids, 44 g carbohydrate, and 1.5 g fat; 300 kcal/day). a decline of body weight to 88% and of serum t3 to 70% of the pretreatment values w ... | 1978 | 634142 |
| [determination of normalized t4 as a screening method for congenital hypothyroidism]. | 1978 | 634243 | |
| [serum concentrations of thyroid hormones in severe non-thyroidal illnesses (author's transl)]. | pathologically low serum total triiodothyronine (t3) concentrations are a characteristic finding in patients with severe non-thyroidal illnesses. no adequate explanation has yet been offered for this phenomenon. we have, therefore, investigated the serum concentrations of total thyroxine (t4), total t3 and total 3,3',5'-triiodothyronine (reverse t3)--the metabolically-inactive metabolite of thyroxine--and of tsh in 13 patients with acute myocardial infarction, in 12 patients with compensated liv ... | 1978 | 636438 |
| the free triiodothyronine (t3) index. | in a large number of normal men (n = 111) and women (n = 110) the free triiodothyronine (t3), index, calculated from serum total t3 and t3-uptake, was highly correlated with free t3, measured by equilibrium dialysis. the correlation was almost as high as that of the free thyroxine (t4) index with free t4. the correlations of the total t3, free t3, and free t3 index with, respectively, the total t4, free t4, and free t4 index were much lower, though still statistically significant. the free t3 in ... | 1978 | 637426 |
| an analysis of factors in the successful management of cancer of tonsillar region. | a total of 117 consecutive primary cancers of the tonsillar region was irradiated at the university of illinois hospitals from 1955 to 1973. results with 94 patients treated with radiotherapy alone and 23 with a combination of surgery and radiotherapy were analyzed. the modalities of radiation used were electron beam, 22 mev photons and cobalt-60. lymph node metastases were noted in 70% of the patients at the initial examination. in patients treated with radiation alone the determinant 5-year su ... | 1978 | 638946 |
| immune and non-immune t4 radioassays utilizing albumin magnetic microparticles. | albumin magnetic microparticles reversibly adsorb thyroxine. they quickly establish equilibrium allowing time and temperature independent measurements in t4 radioassays. we used these particles to compare the efficiency of naoh, hcl, pepsin, sodium trichloroacetate, and 8-anilino-1-naphthalene sulfonic acid to release 125i-t4 from serum, t4-free serum and human serum albumin. we found that the efficiency of the reagents to extract 125i-t4 depended on the concentration and type of proteins to whi ... | 1978 | 639318 |
| effects of iodides on the hypothalamic-pituitary-thyroid axis in neurological endemic cretinism: evidence for compensated thyroidal failure in adult life. | thyroid function studies, performed after iodide administration to five patients with neurological endemic cretinism, were indicative of hypothyroidism. all five subjects had either a low serum thyroxine (t4) or a high basal thyrotrophin (tsh) level and a clearly exaggerated tsh response to thyrotrophin releasing hormone (trh). these findings are in sharp contrast with those we have previously described in goitrous patients without cretinism from the same geographical area. one interpretation of ... | 1978 | 639333 |
| thyroid function and blood pressure in two new strains of spontaneously hypertensive and normotensive rats. | 1. the influence of thryoid function on the development of hypertension was studied in strains of spontaneously hypertensive (sh) and normotensive rats. 2. surgical thyroidectomy decreased systolic blood pressure more markedly in sh rats than in normotensive rats. the effects of oral administration of 5 and 100 micrograms of thyroxine 24 h-1 100 g-1 were studied in the thyroidectomized animals. in the two strains the blood pressure returned to control levels only after administration of the larg ... | 1978 | 639469 |
| the structure of phage t4 lysozyme in solution noticeably differs from its crystalline struction. | 1978 | 639977 | |
| the optic nerve of the brush-tailed possum, trichosurus vulpecula: fibre diameter spectrum and conduction latency groups. | the principal findings of this report on the morphology and electrophysiology of the possum optic nerve are: (i) there are about 230,000 fibres in the optic nerve. this fibre count, based on electron microscopy, is slightly less than a previously reported estimate of the total number of ganglion cells in the possum retina. (ii) the majority (greater than 98%) of the fibres of the optic nerve are myelinated axons of retinal ganglion cells. the diameters of these fibres range from 0.4--4.6 microme ... | 1978 | 641234 |
| complete primary structure of the small outer capsid (soc) protein of bacteriophage t4. | 1978 | 642009 | |
| head maturation pathway of bacteriophages t4 and t2. iii. isolation and characterization of particles produced by mutants in gene 17. | we have isolated and characterized two types of particles produced in comparable amounts by mutants in gene 17: the empty large particle and the empty small particle. dimensions, morphology, stability, and protein composition of the empty large particle are very similar to those of the capsids or empty heads of mature phage. the other type of particle (empty small particle) is very similar in dimensions and stability to the prehead, but differs in that it is composed of processed proteins (gp23, ... | 1978 | 642076 |
| screening for congenital hypothyroidism. results in the newborn population of new england. | during the first year of operation, the new england regional hypothyroidism screening program determined the concentration of thyroxine (t4) levels in dried blood on filter paper from 129,028 infants born in connecticut, maine, massachusetts, new hampshire, and rhode island. specimens from approximately 3,800 neonates were found to have levels of t4 below the lower limit of normal (less than 6.0 microgram/dl) and were assayed for thyroid-stimulating hormone (tsh) content. levels of tsh were elev ... | 1978 | 642171 |
| [determinations of plasma tsh, t4, t3, cortisol and hgh in 2 anencephalic newborn infants]. | 1978 | 642898 | |
| [breast cancer in the male (author's transl)]. | the prognosis of breast cancer in the male is considerably worse than in the female. the longer neglect and the quicker formation of metastases are responsible for it. modified radical mastectomy is the procedure of choice up to stage t3. in stage t4 only mastectomy should be performed. radiotherapy with the megavolt technique is necessary in any case. the experience with castration, adrenalectomy and hypophysectomy is limited, further experience must be awaited. | 1978 | 645271 |
| t3 thyrotoxicosis in a child. | we report the case of a 5-year-old hyperthyroid boy with normal serum t4 levels are elevated serum t3 levels. the findings are diagnostic of t3 thyrotoxicosis, a condition that has been very infrequently reported in childhood. we postulate that low dietary iodine intake may be an etiologic factor in some cases of t3 thyrotoxicosis. | 1978 | 645654 |
| metastatic squamous cell carcinoma of the lip. | squamous cell carcinoma of the lip is a common lesion that is not always acknowledged as a potentially lethal disease. we reviewed the cases of 27 patients with metastatic squamous cell carcinoma of the lip. our data confirm that patients in an older age group are at greater risk for development of metastases than the general population of patients with squamous cell carcinoma of the lip. we also found a universally grave prognosis for patients who exhibit fixation of tumor to the mandible or er ... | 1978 | 646724 |
| a unified theory of nucleation-rate-limited dna renaturation kinetics. | dna renaturations under nucleation-rate-limiting conditions on simple dna such as bacterial and bacteriophage dna show significant deviation from ideal second-order kinetics when followed by optical density measurements at 260 nm. ideal second-order kinetics yield linear plots when the data is plotted in the standard reciprocal second-order (rso) manner. the observed deviations from ideal second-order behavior take the form of steadily downward-curving rso plots. in this paper, experiments are p ... | 1978 | 647102 |
| [determination of serum thyroxine by t4 peg ria-pack]. | 1978 | 648000 | |
| propranolol withdrawal and thyroid hormones in patients with essential hypertension. | the effect of abrupt withdrawal of propranolol on serum concentrations of triiodothyronine (t3) and thyroxine (t4) was investigated in 5 patients with uncomplicated essential hypertension. the patients had been treated from 2 to 18 mo before the study was begun. doses varied from 160 to 480 mg propranolol daily. four of the patients studied developed tachycardia, sweating, or tremor within 2 to 6 days after withdrawal of propranolol. in 1 patient reversible ischemic ecg changes were recorded. th ... | 1978 | 648076 |
| age-related glucose intolerance in hyperthyroid patients. | in an attempt to study age-related metabolic abnormalities, glucose intolerance and serum insulin were examined in normal subjects and hyperthyroid patients. for comparison, serum concentrations of thyroxine (t4), triiodothyronine (t3), and total cholesterol were also measured in normal subjects and hyperthyroid patients. although serum t4 concentration remained unchanged, serum t3 concentration decreased significantly in an elderly group of normal subjects. similarly, serum t4 did not change wi ... | 1978 | 648744 |
| the effect of thyroid hormones on rat pineal indoleamine metabolism in vitro. | the effect of tsh, triiodothyronine (t3) and thyroxine (t4) on basic and noradrenaline (na)-induced indoleamine metabolism was investigated in rat pineal culture based on the observation that, under the experimental system used, melatonin is formed from its amino acid precursor tryptophan. when added to the culture medium, alone or in the presence of na, tsh in dosages of 5 and 20 mu had no significant effect on pineal tryptophan metabolism.t4 and 2.5 microgram increased the melatonin concentrat ... | 1978 | 650203 |
| neonatal thyrotoxicosis: alterations in serum concentrations of lats-protector, t4, t3, reverse t3, and 3,3't2. | 1978 | 650321 | |
| binding of the structural protein soc to the head shell of bacteriophage t4. | 1978 | 650689 | |
| [results of therapy in cases of tumors of the nasal cavity and paranasal sinuses in relation to different tumor classifications (author's transl)]. | the primary findings in 128 patients with malignancies of the nasal cavity and the paranasal sinuses were classified by 4 tumor classifications. the survival times and the rates of recurrences in relation to the stage of tumor were compared. mostly cancers of advanced stages t3 and t4 with bad prognosis were treated: only 35% of the examined patients survived longer than 5 years. there was no obvious difference in the results of therapy in tumor cases t3 and t4 in relation to the different tumor ... | 1978 | 651499 |
| [study of thyroxine synthesis in non-toxic goiters using dispersed thyroid cells (author's transl)]. | the ability of thyroid tissue from non-toxic goiter to synthesis thyroid hormones was investigated by measuring 131 i-iodide incorporation in t4 in dispersed thyroid cell system. dispersed thyroid cells were prepared by trypsinization from 5 pathological and 2 normal thyroid specimens. the patients with non-toxic goiter were euthyroid or in mild hypothyroidism. the capacity of t4 synthesis was decreased in 4 cases and increased in 1 as compared to that of normal thyroid tissue. in one case, the ... | 1978 | 655613 |
| [interference of t4 in different serum t3 radioimmunologic determination kits]. | the cross reactivity of t3 antibodies for t4 was studied with five t3 ria kits. t4 used was provided from t4 ria kits (abbot beckman, corning). the results are shown on the table indicating the concentration of t3 or t4 required to displace 50% of the t3 125 from anti t3. the cross reactivity for t4 of t3 antibodies from lepetit and phadebas was the same and was smaller than cross reactivity with the other laboratories. in clinical practice serum t4 levels of hypo and euthyroid subjects are not ... | 1978 | 655615 |
| [in vitro synthesis of 3,5,3'-triiodothyronine catalyzed by thyroid peroxidase (author's transl)]. | 3,5,3'-triiodotyronine formation was studied in vitro after iodination of goiter thyroglobulin catalyzed by purified thyroid peroxidase. a fractionnal number of t3 is always obtained per mole of thyroglobulin in both in vitro and in vivo. this result cannot be explained by a heterogeneity in thyroglobulin iodination or in a partial conversion of t3 to t4. it is suggested that thyroglobulin is heterogenous either in its primary sequence or in its teritiary configuration. thyroglobulin contains ty ... | 1978 | 655617 |
| [fetal and neonatal triiodothyronine (author's transl)]. | thyroid hormones of amniotic fluids give informations about their presence in the fetus. t3 and t4, determined by radioimmunoassay, are found as soon as 12 weeks of gestation. their amounts are higher at 17 weeks than at 30-40 weeks of gestation. thyroid tissues of premature and full term neonates were analysed by chromatography after hydrolysis and 127i determined. t3 amount was very low near birth and increased with duration of survival. these results may be related to hormonal serum changes o ... | 1978 | 655618 |
| [serum t4, t3, rt3 and t.s.h. concentration in cancer (author's transl)]. | 1978 | 655619 | |
| [the low serum triiodothyronine syndrome in nonthyroid diseases. distribution of plasma tsh concentrations]. | t3 serum concentrations (ria) was low in 25 cases and normal low in 10 among 57 patients with serious systemic illnesses. these 35 patients were in clinical euthyroid state and had a normal t4 serum concentration and f.t.i. t.s.h. serum concentration was normal in 28 cases of 31 low t3 syndrome observed. | 1978 | 655621 |
| effect of atp analogues on t4 polynucleotide ligase. | 1978 | 656099 | |
| use of ethidium bromide fluorescence enhancement to detect duplex dna and dna bacteriophages during zone sedimentation in sucrose gradients: molecular weight of dna as a function of sedimentation rate. | duplex dna molecules and dna bacteriophages have been sedimented through 5--25% sucrose gradients containing ethidium bromide. the location of dna within the gradients has been determined by illuminating gradients with ultraviolet light and observing the ethidium bromide fluorescence enhancement induced by the dna. the relative sedimentation rates of linear, duplex dnas from bacteriophages t4, t5, t7 and an 8.3% t7 deletion mutant have been determined. the distances sedimented by dna have been c ... | 1978 | 656381 |
| excision repair of ultraviolet damage in mammalian cells. evidence for two steps in the excision of pyrimidine dimers. | the incidence of pyrimidine dimer formation and the kinetics of dna repair in african green monkey kidney cv-1 cells after ultraviolet (uv) irradiation were studied by measuring survival, t4 endonuclease v-sensitive sites, the fraction of pyrimidine dimers in acid-insoluble dna as determined by thin layer chromatography (tlc), and repair replication. cv-1 cells exhibit a survival curve with extrapolation number n = 7.8 and do = 2.5 j/m2. pyrimidine dimers were lost from acid-insoluble dna more s ... | 1978 | 656544 |
| red cell 2,3-diphosphoglycerate and oxygen affinity of hemoglobin in patients with thyroid disorders. | we measured red blood cell 2,3-diphosphoglycerate (2,3-dpg), adenosine triphosphate (atp), and the p50 value in vitro of the oxyhemoglobin dissociation curve, which is the oxygen tension at half saturation of hemoglobin, in order to quantitate red blood cell oxygen transport function in individuals who were diagnosed as hypothyroid, euthyroid, or hyperthyroid based on measurements of thyroxine (t4), triiodothyronine (t3), thyrotropin (tsh), and their clinical status. hypothyroid (mean t4 2.8 mic ... | 1978 | 656626 |
| investigations on the deiodination of thyroxine (t4) to 3,3'-diiodothyronine (3,3'-t2) in rat liver homogenate. | properties of an inactivating pathway of t4 to 3,3'-diiodothyronine (t2') in rat liver homogenate are described. the intermediate product (of this reaction sequence) is reverse t3 (rt3) which is very labile and cannot be measured at ph 7.5. the apparent km of the reaction rt3 to t2' is 2 x 10(-8) m. the activities catalyzing the reactions t4 to t3, t4 to t2' and rt3 to t2' are found in the 100 000 x g pellet. propylthiouracil inhibits all three activities to a similar degree; alpha-methyl-p-tyro ... | 1978 | 657520 |
| [serum t4 determination with thyroxine (t4) "eiken"--experimental and clinical studies]. | 1978 | 657558 | |
| uv-induced mutation in bacteriophage t4. | two late gene am mutants of bacteriophage t4 that can be induced to revert by uv were crossed to a temperature-sensitive ligase mutant. in the double mutants, uv-induced reversion was eliminated at a semirestrictive temperature. when the single am mutants were irradiated and then allowed a single passage in a permissive host, the uv-induced reversion frequency was increased by 15- to 25-fold. this increased mutagenesis was also abolished by the presence of the ligase allele. when the uv-irradiat ... | 1978 | 660716 |
| head maturation pathway of bacteriophages t4 and t2. iv. in vitro transformation of t4 head-related particles produced by mutants in gene 17 to capsid-like structures. | t4 mutants in gene 17 accumulate particles which contain the main head protein in the cleaved form (gp23*) arranged in an unexpanded lattice (empty small particles), together with other expanded capsids (empty large particles). the isolated empty small particles can be transformed in vitro, by lowering the ionic strength, to capsid-like structures. this structural transformaton is not coupled to chemical modification of the structural proteins of the empty small particles. in contrast to unexpan ... | 1978 | 660719 |
| [neonatal screening for hypothyroidism. preliminary results from different methods (author's transl)]. | in a regional study 2062 newborns were screened for congenital hypothyroidism by determination of tsh in plasma or dried blood spots on the fifth day of life. three newborns with congenital hypothyroidism were detected. all of them showed high tsh-levels between 462 and 2192 micromicron/ml. one newborn with a congenital goiter had a tsh-level of 143 micromicron/ml. additionally, in a follow-up study of 50 newborns with jaundice tsh, t4, t3 and reverse t3 during the first 120 h of life were measu ... | 1978 | 661833 |
| in vitro transcription and isolation of a polycistronic rna product of the t4 trna operon. | 1978 | 661965 | |
| [fluorescent thyroid scanning and intrathyroidal iodine quantification: study in 140 patients (author's transl)]. | the system consists of twenty individual sorces of one ci of 241am and a high resolution lithium drifted silicon detector. a dual channel analyser permits to measure intrathyroidal stable iodine. the scan requires only 13 minutes and can be made with a very low radiation dose (less than 10 mrads). it provides good quality images and remains available in cases of flooded iodine pools or iodine induced thyrotoxicosis. it is of great interest to follow up subacute thyroiditis and to diagnose autono ... | 1978 | 662595 |
| [determination of serum thyroxine using ria-gnost t4 (author's transl)]. | 1978 | 663324 | |
| myxedema coma and normal serum thyroxine. | a 76-year-old man who developed the clinical syndrome of myxedema coma was found to have a normal serum thyroxine concentration. further investigation revealed the presence of a low serum t3 level before and after sodium levothyroxine therapy when the serum t4 value was in the hyperthyroid range. in addition, he demonstrated an inability to release tsh after intravenous administration of trh and was found to have an enlarged sella turcica. a partial defect in peripheral conversion of t4 to t3 is ... | 1978 | 663737 |
| antigenic gene products of bacteriophage t4 baseplates. | 1978 | 664235 | |
| [technical realization of the t3 and t4 tests as well as of the triiodothyronine radioimmunoassay]. | 1978 | 664812 | |
| thyroid disease and function in breast cancer patients and non-hospitalized controls evaluated by determination of tsh, t3, rt3 and t4 levels in serum. | investigations concerning a possible influence of thyroid disease and function on the risk for breast cancer have given contradictory results--although a slight hypothyroidism was repeatedly suggested and supported by increased thyrotropin concentrations and exaggerated trh-responses. the selection of proper controls was stressed and in this study 179 consecutively diagnosed breast cancer patients were compared with 179 age-matched controls chosen from a computerized population register. the pre ... | 1978 | 665106 |
| disturbed circadian variation of serum thyrotropin in patients with endogenous depression. | the relationship between the degree of depression and the circadian variation of serum tsh, t3 and t4 was investigated in 19 endogenously depressed patients. the difference between the hormone concentrations at 2 p.m. and at 12 p.m. was taken as an estimate of the magnitude of circadian variation. it was found that the circadian variation in serum tsh was inversely related to the degree of endogenous depression. this was mainly due to a diminution or absence of the night increase of tsh in sever ... | 1978 | 665284 |