Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| comparison of hepatitis b virus subtyping of d/y determinants by radioimmunoprecipitation assay and the polymerase chain reaction. | using a double polymerase chain reaction a method was devised for detecting and subtyping hepatitis b virus dna in serum samples. primers from the s-gene were selected from the sequence analyses of five hbv hbsag subtypes, to amplify hbv dna and subtype for y specific dna. thirty-eight samples were subtyped for d and y determinants by radioimmunoprecipitation assay (ripa) and the polymerase chain reaction (pcr). subtyping by pcr and ripa was in agreement in 100% of subtype y samples and 83.3% of ... | 1992 | 1349339 |
| lack of suppression by ribavirin of hiv viraemia. | 1992 | 1351573 | |
| loss of heterozygosity on chromosome 16 in hepatocellular carcinoma. | in order to understand the molecular genetics of human hepatocellular carcinoma (hcc) a common chromosomal abnormality in hcc was examined using restriction fragment length polymorphism (rflp) analysis. sixty-eight hcc specimens were examined for loss of heterozygosity at 11 different chromosomal arms including 1p, 5p, 11p, 11q, 13q, 14q, 15q, 16p, 16q, 18q, and 19q. losses were not detected on chromosome 1, 5, 14, 15 or 19, and the frequencies of losses were low (11-19%) for chromosomes 11, 13 ... | 1992 | 1351753 |
| genetic control of nonresponsiveness to hepatitis b virus vaccine by an extended hla haplotype. | we previously reported evidence for a statistical association between the serologically determined hla-bw54, dr4 and drw53 alleles and the non-immune responsiveness to hepatitis b virus surface antigen (hbsag) in the japanese population. to identify the locus and allele within the hla region associated with the nonresponsiveness to hbsag, serological hla typing, dna typing of hla-dq and dp alleles using amplified hla genes and sequence-specific oligonucleotide probes, and restriction fragment le ... | 1992 | 1352502 |
| activation of multidrug resistance (p-glycoprotein) mdr3/mdr1a gene during the development of hepatocellular carcinoma in hepatitis b virus transgenic mice. | the expression of multidrug resistance (mdr) genes was investigated in the livers of transgenic mice that express the human hepatitis b virus large envelope polypeptide under the transcriptional control of a liver-specific promoter. these mice develop a storage disease due to the accumulation of a nonsecretable form of hepatitis b surface antigen in the hepatocyte. liver cell injury is followed by a hepatocellular proliferative response, dysplasia, microscopic nodular hyperplasia, and finally he ... | 1992 | 1356418 |
| recognition efficiency of the hepatitis b virus polyadenylation signals is tissue specific in transgenic mice. | the hepatitis b virus genome contains a unique polyadenylation (tataaa) signal which is differentially utilized in the formation of the various hepatitis b virus transcripts. a head-to-tail multiple-copy insertion of a viral fragment comprising the viral enhancer, the x promoter, the x open reading frame, and the viral poly(a) signal in transgenic mice allowed us to monitor tissue-specific differences in the expression of transcripts initiating from the x promoter. these transcripts are efficien ... | 1992 | 1357192 |
| secretory immunoglobulins in serum from human immunodeficiency virus (hiv)-infected patients. | infection by the human immunodeficiency virus is associated with polyclonal b cell activation and increased levels of serum iga. in order to characterize the molecular species of serum iga, we have measured total iga, iga1, and iga2 in sera from 60 hiv-1-infected patients and 40 healthy controls. in addition, secretory iga (s-iga), secretory igm (s-igm), free immunoreactive secretory component (sc), and the distribution of monomeric and polymeric iga were determined. the data confirm the elevati ... | 1992 | 1358913 |
| cross-contamination potential with dental equipment. | some types of reused dental equipment, especially handpieces and their attachments for drilling and cleaning teeth, might be responsible for cross-contamination if patient material were to lodge temporarily in difficult-to-disinfect internal mechanisms. this possibility is worrisome with respect to transmission of hepatitis b and human immunodeficiency viruses (hbv, hiv). previous cross-contamination studies have relied on laboratory experiments with bacteria or dye tracers. to assess possible r ... | 1992 | 1359320 |
| different dna changes in primary and recurrent hepatocellular carcinoma. | dna restriction fragment length polymorphism analysis was carried out on a primary and recurrent hepatocellular carcinoma in a hepatitis b virus negative patient. for the primary tumour, allele losses were found on the short arm of chromosome 17 (probe: p144-d6, 17p13) and the long arm of chromosome 5 with the probe lambda ms8 (5q35-qter); other probes showed either no allele loss or a non-informative pattern. the recurrent cancer also showed allele loss with p144-d6, but not with lambda ms8. in ... | 1992 | 1359992 |
| hepatitis b virus infection among aboriginal children in eastern taiwan. | hepatitis b virus (hbv) infection is endemic in taiwan, with 15-20% of the adult population carrying the surface antigen (hbsag). hualien in eastern taiwan is relatively isolated from other parts of the island by the central mountains. the status of hbv infection among mountain aborigines in hualien has not been studied on a large scale before. a survey of serum hbsag and its antibody (anti-hbs) was conducted in 1989. a total of 3,287 children from 27 primary schools in three mountainous countie ... | 1992 | 1360296 |
| up-regulation of intercellular adhesion molecule 1 transcription by hepatitis b virus x protein. | intercellular adhesion molecule 1 (icam-1), a counter-receptor for lymphocyte function-associated antigen 1 on t cells, is critically important to a wide variety of adhesion-dependent leukocyte functions, including antigen presentation and target cell lysis. icam-1 expression by hepatocytes is increased in areas of inflammation and necrosis during chronic hepatitis b. whether induction of icam-1 is due to the effect of inflammatory cytokines or involves a direct effect of the hepatitis b virus ( ... | 1992 | 1360668 |
| molecular mimicry of hepatitis b surface antigen by an anti-idiotype-derived synthetic peptide. | monoclonal antibody 2f10 is an "internal-image" anti-idiotype (anti-id) antibody capable of mimicking the group-specific "a" determinant of human hepatitis b surface antigen (hbsag). by mrna sequencing and computer-assisted molecular modeling of monoclonal antibody 2f10, we identified a 15-amino acid region of the heavy-chain hypervariable region that has partial residue homology with sequences of the "a" determinant epitopes of hbsag. we have established that a linear 15-mer peptide from a cont ... | 1992 | 1361231 |
| hepatitis b vaccine alone or in combination with anti-hbs immunoglobulin in the perinatal prophylaxis of babies born to hbsag carrier mothers. | the efficacy of hepatitis b virus (hbv) vaccine alone (group i) or in combination with hepatitis b immunoglobulin (hbig) (group ii) for prevention of perinatal transmission of the virus was assessed in 21 and 24 neonates, respectively. 58 infants who could not be vaccinated constituted the control group. it was observed that in the unvaccinated group approximately 70% of the infants became infected. in both the vaccinated groups, the seroconversion and seroprotection rates (anti-hbs > or = 10 iu ... | 1992 | 1362320 |
| the mechanisms and risks of surgical glove perforation. | intact surgical gloves are a barrier to hepatitis b virus and human immunodeficiency virus (hiv) but once perforated during surgery they cannot sustain adequate defence. this study examines the rate of glove perforations during surgery at a district general hospital. in total, 275 pairs of gloves were collected from 100 consecutive operations. in the 43% of gloves that had been damaged 200 perforations were recorded. the mean rate per operation in the surgeon's gloves was 1.18. injuries to the n ... | 1992 | 1363107 |
| [periarteritis nodosa and an infection with the hepatitis b virus]. | 1992 | 1363449 | |
| treatment of polyarteritis nodosa and churg-strauss syndrome. a meta-analysis of 3 prospective controlled trials including 182 patients over 12 years. | to define the most effective treatment for polyarteritis nodosa (pan) and churg-strauss syndrome (css), we undertook 3 consecutive prospective therapeutic trials including 182 patients and tried to answer several important questions: should cyclophosphamide (cyc) be given as the first-line treatment? what is the place of plasma exchanges (pe) in the treatment of systemic vasculitis? does hepatitis b virus (hbv)-related pan require specific treatment? our first randomized trial in 71 patients com ... | 1992 | 1363508 |
| polyarteritis nodosa related to hepatitis b virus. a retrospective study of 66 patients. | in an attempt to establish the characteristics, circumstances leading to infection and development of polyarteritis nodosa (pn) related to hepatitis b virus (hbv), prognostic factors and outcome, and to define the most effective treatment, 66 patients observed between 1972 and 1989 were analyzed. hepatitis was clinically present in 19/66 patients before pn. in most cases, pn occurred less than 6 months after infection. clinical manifestations of pn were comparable to those observed in patients w ... | 1992 | 1363769 |
| relationship between tuberculin reactivity and hepatitis b virus infection in the northwest territories. | of 370 nwt inuit or dene who were hbsag-positive, only 31 had hbeag. persons who were ppd negative were 6.2 times more likely to be hbeag-positive than those who were ppd positive, but this inverse association applied only to those 30 years of age. we further analyzed a group of 3,378 inuit whose status was known for hbv serologic markers and ppd reactivity. overall, a significantly greater proportion (76.2%) of hbsag positives were ppd reactive in comparison to those positive for anti-hbs (67.2 ... | 1991 | 1365158 |
| ultrastructure of normal and hepatitis virus infected human and chimpanzee liver: similarities and differences. | ultrastructure of liver biopsy specimens obtained from normal and hepatitis b (hbv) and hepatitis c virus (hcv) infected livers of patients and chimpanzees were compared. nuclear alterations (glycogen particles, nuclear bodies, "vermicellar bodies", etc.), intracytoplasmic crystalloid inclusions were observed before and after the hbv and hcv infections both in human and chimpanzee hepatocytes, however, some of them were more common during the viral infection. extreme endoplasmic reticulum dilata ... | 1992 | 1365764 |
| [hepatitis c viral infection and the consumption of intravenous drugs]. | to know the antibodies seroprevalence against hepatitis c virus (hcv), as well as other factors associated to these antibodies presence in a group of intravenous drug users (ivdu) in baleares. to know what screening test would be the most adequate to detect antibodies against hcv in this group. | 1992 | 1366219 |
| [the spread of human immunodeficiency virus infection (hiv-1/hiv-2) and of hepatitis b and c in non-drug-addicted prostitutes. extremadura]. | the proportion of cases of aids in extremadura, in which sexual transmission has existed, is comparatively lower than the national one. the possibilities of the infection introduction in the general population may increase the relative importance of this way of transmission in the future. penetration of hiv is unknown in prostitutes living and working in the autonomous community of extremadura, as a collective associated to virus sexual transmission and to other socio-cultural risk factors invol ... | 1992 | 1366224 |
| [immunogenicity of the vaccine against hepatitis b virus in schoolchildren of madrid]. | we carried out a vaccine program against hepatitis b in scholar population aged 5 to 17 educated in 3 institutions of madrid city/ region. those children who followed the vaccination criteria (negative hbsag and anti hbc markers) were vaccinated. the vaccination regimen (engerix b recombinant dna vaccine) was 0-1-6 months and dose was 10 and 20 ug i.m. for those aged 6 to 13 and 13 to 17 respectively. the postvaccine serologic results (anti-hbs titer) was evaluated one month after tha last dose, ... | 1992 | 1366248 |
| [chronic changes in the liver in our patients]. | vhb infection together with alcohol ist the most important ethiologic factor in the genesis of chronic liver parenchyma damage in our sample. selection of patients with chronic diseases of the liver should be done on the basis of hepatitis markers determination, that is on the presence of hbsag and hbeag and the activity of aminotransferases in blood. doing this it can be seen that more that half of the patients with chronic liver damage do not need therapy, and they have work capacity preserved ... | 1991 | 1366324 |
| a yeast system for stable expression of hepatitis b surface antigen. | we have constructed a yeast strain that has integrated into its chromosomal ribosomal rna gene site two copies of hepatitis b virus surface (hbs) antigen gene under the control of the yeast (saccharomyces cerevisiae) glyceraldehyde-3-phosphate dehydrogenase (gap) promoter and terminator. the level of expression of hbs gene was low in the strain, but upon chemical and physical mutageneses, in combination with an immunological screening procedure, a mutant clone which expressed hbs protein at a hi ... | 1990 | 1366913 |
| improvement in the proliferative activity of human-human hybridomas at low cell density by transfection with bfgf gene. | highly purified recombinant basic fibroblast growth factor (rbfgf) and acidic fgf (afgf) stimulated the proliferation of human-human (h-h) hybridomas to the extent of over four-fold from a low cell density such as 1 x 10(3) cells per ml in a serum-free medium in 24-well plates. the stimulatory effect of rbfgf was also observed in various lymphoid cell lines. expecting that fgf could be an autocrine growth factor, we introduced bfgf gene into a h-h hybridoma using an expression plasmid induced by ... | 1991 | 1367409 |
| expression of the core antigen gene of hepatitis b virus (hbv) in acetobacter methanolicus using broad-host-range vectors. | using the broad-host-range promoter probe vector prs201 for cloning of phage acm1 promoters, we established a convenient vector system for expression of heterologous genes in different gram-negative bacteria. the usefulness of this system was demonstrated by expression of the hbv core gene in acetobacter methanolicus. plasmids carrying the hbv core gene downstream of different acm1-phage promoters were transferred to a. methanolicus, a new potential host for recombinant dna expression. using enz ... | 1991 | 1367579 |
| comparison of culture methods for human-human hybridomas secreting anti-hbsag human monoclonal antibodies. | human-human hybridomas which secrete a human monoclonal antibody (h-moab) against hepatitis b virus surface antigen showed growth associated production kinetics. the rate of h-moab production rapidly decreased after cell growth was arrested in a perfusion culture, even if the perfusion rate was increased. a continuous suspended-perfusion culture, in which both culture broth and culture supernatant are continuously harvested and the same volume of fresh medium is continuously fed into the reactor ... | 1991 | 1367664 |
| fermentation study for the production of hepatitis b virus pre-s2 antigen by the methylotrophic yeast hansenula polymorpha. | various physico-chemical parameters have been studied in order to improve the production of hepatitis b virus pre-s2 antigen (middle surface antigen) by the methylotrophic yeast hansenula polymorpha. antigen production was done in two steps: first, production of cells on glycerol (phase 1), followed by induction of antigen expression with methanol (phase 2). dense cultures of h. polymorpha, equivalent to 35-40 g/l (dry weight), were readily obtained in small fermenters using minimal medium conta ... | 1991 | 1367897 |
| some factors influencing the proportion of periplasmic hepatitis b virus pre-s2 antigen in the recombinant yeast hansenula polymorpha. | a central composite design (ccd) was used to evaluate, for the purpose of future process optimization, the influence of ph, yeast extract and ammonium chloride concentrations on the proportion of periplasmic hepatitis b pre-s2 antigen in the recombinant yeast hansenula polymorpha. each factor was tested at five levels, and a second order polynomial model for the proportion of periplasmic antigen was fitted to the treatment combinations. ph showed the greatest effect: the proportion of periplasmi ... | 1992 | 1367976 |
| influence of ph, nitrogen and phosphorus sources on the production of hepatitis b virus pre-s2 antigen by hansenula polymorpha. | experimental design techniques were used to study the influence of the composition of the culture medium on the production of hepatitis b virus pre-s2 antigen by the methylotrophic yeast hansenula polymorpha. ph, phosphoric acid, ammonium chloride and yeast extract concentrations were selected as experimental factors and their influence was investigated using central composite design techniques. the results indicated that antigen yield was maximized at high ph and in a culture medium containing ... | 1992 | 1369139 |
| characterization of two differently glycosylated molecular species of yeast-derived hepatitis b vaccine carrying the pre-s2 region. | modified hepatitis b virus surface antigen m protein particles (hbsag m-p31c) produced in yeast is mainly composed of two differently glycosylated proteins, gp37 and gp34. gp37 has an n-linked sugar chain and o-linked sugar chains; and gp34 has an n-linked sugar chain bound to the peptide backbone p31. although m-p31c vaccine elicits both anti-s and anti-pre-s2 antibodies, whether there are any differences between gp37 and gp34 in the ability to elicit these antibodies is still unknown. to clari ... | 1992 | 1369148 |
| identification of polymerized-albumin receptor domain in the pre-s2 region of hepatitis b virus surface antigen m protein. | the pre-s2-coding region in the hepatitis b virus surface antigen m (p31; pre-s2 + s) protein gene was modified to identify a polymerized-albumin receptor (par) domain by deleting restriction fragments or performing site-directed mutagenesis. the modified m protein genes (m-p31x; x = d, e, f, h and i) were cloned into the yeast generalized-expression vector pgld 906-1 and expressed in saccharomyces cerevisiae under the control of yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. the ... | 1992 | 1369362 |
| enhancement of gene expression by somatic hybridization with primary cells: high-level synthesis of the hepatitis b surface antigen in monkey vero cells by fusion with primary hepatocytes. | vero cells transfected with the s gene encoding the surface antigen (hbsag) of the hepatitis b virus (hbv) synthesize hbsag at low levels. we have obtained a large increase in s gene expression by somatic hybridization of vero cells with primary hepatocytes, which are the natural target cells for hbv infection. fusion with cells other than hepatocytes did not enhance expression of the s gene. the vero/hepatocyte hybrid clones analyzed are stable and have maintained a high level of hbsag synthesi ... | 1990 | 1370009 |
| a genetic system to elicit and monitor antipeptide antibodies without peptide synthesis. | we present a simple and flexible procedure to elicit and assay anti-peptide antibodies without peptide synthesis. it consists of expressing the peptide of interest in the form of a genetic insert within two different "recipient" bacterial proteins. one hybrid protein is used as immunogen for the induction of antibodies against the inserted peptide and the other as antigen for monitoring the anti-peptide antibodies raised. the two "recipient" proteins used are the male and the lamb proteins from ... | 1991 | 1370036 |
| the position of heterologous epitopes inserted in hepatitis b virus core particles determines their immunogenicity. | the nucleocapsid (hbcag) of the hepatitis b virus (hbv) has been suggested as a carrier moiety for vaccine purposes. we investigated the influence of the position of the inserted epitope within hybrid hbcag particles on antigenicity and immunogenicity. for this purpose, genes coding for neutralizing epitopes of the pre-s region of the hbv envelope proteins were inserted at the amino terminus, the amino terminus through a precore linker sequence, the truncated carboxy terminus, or an internal sit ... | 1992 | 1370083 |
| hepatitis b virus envelope l protein particles. synthesis and assembly in saccharomyces cerevisiae, purification and characterization. | the hepatitis b virus envelope gene encodes three transmembrane proteins in frame; s, the product of s gene; m, the product of m (pre-s2 + s) gene; and l, the product of l (pre-s1 + pre-s2 + s) gene. unlike the s and m proteins, attempts to efficiently synthesize l proteins and assemble them into l protein particles in various eukaryotic cells have been unsuccessful, probably because of the presence of the pre-s1 peptide with an unknown function which appears to be inhibitory to the host secreto ... | 1992 | 1370486 |
| seroprevalence of hepatitis c virus nucleocapsid antibodies in patients with cryptogenic chronic liver disease. | the serological responses to two different hepatitis c virus antigens were studied by enzyme-linked immunosorbent assay in a variety of chronic liver diseases and in healthy blood donors. the study population comprised 97 cases of cryptogenic chronic liver disease (40% with a history suggestive of parenterally transmitted non-a, non-b hepatitis and 60% without such a history), 87 cases of other well-characterized chronic liver diseases and 96 voluntary blood donors. the commercially available c1 ... | 1992 | 1370945 |
| expression of the p-protein of the human hepatitis b virus in a vaccinia virus system and detection of the nucleocapsid-associated p-gene product by radiolabelling at newly introduced phosphorylation sites. | hepatitis b virus (hbv) contains a particle-associated dna polymerase/reverse transcriptase activity encoded by the p (pol) open reading frame. due to its low abundance, the corresponding protein has so far escaped direct detection and structural analysis. as a first step to overcome these difficulties, a series of recombinant vaccinia viruses was constructed and used for the synthesis in human hepatoma cells of both the authentic full length protein and of its functional domains. pulse chase ex ... | 1992 | 1371344 |
| analysis of the antigenic epitopes of hepatitis b surface antigen involved in the induction of a protective antibody response. | vaccination with hepatitis b surface antigen (hbsag) has shown that antibody directed against the common 'a' determinant of this antigen is protective against infection with hepatitis b virus (hbv). in this study the antigenic epitopes of the 'a' determinant have been analysed by competitive inhibition assays and by binding studies to synthetic peptides using a panel of monoclonal antibodies prepared against hbsag, all of which are shown to recognise the common group determinant. one murine mono ... | 1992 | 1371369 |
| hepatocellular carcinoma in transgenic mice: a consequence of continued expression of the hbx gene? | 1992 | 1371486 | |
| hepatitis b virus envelope epitopes: gene assembly and expression in escherichia coli of an immunologically reactive novel multiple-epitope polypeptide 1 (mep-1). | a novel synthetic 323-bp gene with the open reading frame of a multiple-epitope polypeptide has been assembled and cloned. the gene is engineered by contiguous alignment of selected epitopes and functional domains of the hepatitis b virus envelope proteins separated by pairs of glycine residues. high-level bacterial production of this 100-amino acid (approx. 10 kda) protein has been achieved and the gene product is stable. elisa and western blot experiments using epitope-specific antisera confir ... | 1992 | 1371488 |
| expression of a hepatitis e virus (hev)-trpe fusion protein containing epitopes recognized by antibodies in sera from human cases and experimentally infected primates. | a 1700 base cdna fragment coding for the putative structural gene(s) of hepatitis e virus (hev) was inserted into the path 10 expression vector. the fusion protein (c2) expressed by this plasmid was found to contain epitopes recognized by anti-hev antibodies. c2 protein was used in a western blot format to examine its usefulness in detecting anti-hev antibodies in well documented human cases of hev and non-human primates infected with hev. both igm and igg anti-hev could be detected in our weste ... | 1992 | 1373282 |
| altered glycosylation of alpha-fetoprotein in hepadnavirus-induced hepatocellular carcinoma of the woodchuck. | altered glycosylation of alpha-fetoprotein (afp) has been proposed as a marker of hepatocellular carcinoma (hcc) in humans. the lectin-binding properties of woodchuck afp were investigated to determine if woodchuck hepatitis virus (whv)-induced hccs are also accompanied by changes in afp glycosylation. ninety-eight to 100% of the afp from normal, whv-free woodchucks with physiologic afp elevations and from whv-carrier woodchucks with hcc bound to concanavalin a, indicating that virtually all of ... | 1992 | 1373341 |
| sero epidemiological characteristics of hepatitis c encountered in general practice in belgium. | a study carried out between 1982 and 1984 established by exclusion diagnosis that 35% of viral hepatitis cases registered in belgium were due to non a, non b (nanb) viruses. recently, a new anti-hepatitis c virus (hcv) detection test was used to analyse the sera of patients in whom nanb hepatitis was diagnosed in that study. using this new serological test for hcv, 29% of the nanb group was found to be positive for anti-hcv. in the 1982-84 study on viral hepatitis diagnosed by general practition ... | 1992 | 1373384 |
| expression of heterologous peptides at two permissive sites of the male protein: antigenicity and immunogenicity of foreign b-cell and t-cell epitopes. | we previously determined a number of 'permissive' sites in the periplasmic maltose-binding protein (male) from escherichia coli. these sites accept the insertion of heterologous peptides without major deleterious consequences for the activities, structure and cellular location of the protein. this study explores the versatility of two such permissive sites for the synthesis of foreign peptides, and examines the antigenicity and the immunogenicity of the inserts. one site is located after amino a ... | 1992 | 1373395 |
| the clinical outcome of hepatitis c virus antibody-positive renal allograft recipients. | in order to investigate the prevalence of antibody to hepatitis c virus (anti-hcv) in renal transplant patients, the evolution of anti-hcv status, and clinical outcome in anti-hcv-positive renal allograft recipients, we tested the sera from 120 renal transplant patients for anti-hcv. thirty-eight patients were hepatitis b surface antigen (hbsag)-positive. two patients were anti-delta-positive. a total of 79 patients (65.8%) had at least one serum positive for anti-hcv. anti-hcv positivity decrea ... | 1992 | 1373534 |
| hepatitis b and hepatitis c in emergency department patients. | infections with hepatitis b virus (hbv), hepatitis c virus (hcv), and the human immunodeficiency virus type 1 (hiv-1) are common in inner-city populations, but their frequency and interrelations are not well established. | 1992 | 1373867 |
| immunoreactivity for c-erbb-2 oncopeptide in benign and malignant diseases of the liver. | the immunohistochemical detection of the c-erbb-2 oncopeptide (p185erbb2) has been shown to be a valid marker for over-expression of this oncogene. to evaluate the possible relevance of gene expression to the proliferation of hepatocytes and bile ducts in human disease, the authors applied a monoclonal anti-p185 antibody to formalin-fixed, paraffin-embedded tissues from 67 examples of benign proliferative and neoplastic hepatic lesions and fetal liver. focal membrane-based reactivity for the onc ... | 1992 | 1374219 |
| prevalence of antibody to hepatitis c virus in japanese schoolchildren: comparison with adult blood donors. | a total of 1,442 schoolchildren in the matsumoto city area were investigated for the prevalence of hepatitis virus-related serum markers, including antibody to hepatitis c virus (anti-hcv) and an abnormal serum transaminase level. despite the large number of children tested, none was positive for anti-hcv antibodies or had been diagnosed as having viral hepatitis. the prevalence of anti-hcv antibodies in children and adult blood donors in the same area increased significantly with age from 0% in ... | 1992 | 1374221 |
| [investigation of anti-hepatitis c virus in the sera of different populations of beijing]. | a study of anti-hcv in the sera of different populations of beijing was conducted. of the general population, 2.1% (9/438) were anti-hcv positive, and of the patients that underwent blood transfusion 11.1% (6/54) were anti-hcv positive. among patients with chronic liver diseases, anti-hcv was positive in 10.5% (36/342) of patients with chronic persistent hepatitis (cph), 12.1% (13/107) of those with chronic active hepatitis (cah), 42.6% (63/148) of those with liver cirrhosis (lc) and 38.4% (20/5 ... | 1992 | 1374286 |
| hcv and hbv infection among multitransfused thalassemics from eastern sicily. | serum specimens from 152 sicilian multitransfused thalassemic subjects were tested for antibodies to hepatitis c virus (anti- hcv) and for hbv markers by enzyme linked immunoassay and with reference to anti-hcv, confirmed by recombinant immunoblot assay. a high rate (47%) of subjects was anti-hcv positive. hbsag was found in 8% of patients and 55% had anti-hbs or anti-hbc antibodies or both. contrary to hbv infection, anti-hcv seropositivity was related to the number of transfused units. the hig ... | 1992 | 1374737 |
| protein composition of the hepatitis b virus e antigen in the natural course of disease and following interferon therapy. | the protein composition of hepatitis b virus (hbv) e antigen (hbeag) in serum was analyzed for 63 viremic patients with chronic hbv and found to consist of polypeptides with molecular masses of 16, 18, and 20 kda (p16e, p18e, and p20e, respectively). several experiments demonstrated their viral nature and hbeag specificity and that p16e occurs either in free soluble form or as aggregates or immunocomplexes, while p18e and p20e occur essentially as immunocomplexes. of 63 patients, 45 (71%) had p1 ... | 1992 | 1374762 |
| detection of acute hepatitis c virus infection by elisa using a synthetic peptide comprising a structural epitope. | an enzyme-linked immunosorbent assay (elisa) was developed by using a synthetic polypeptide (sp) whose sequence was derived from the structural region of hepatitis c virus (hcv). results of several coded panels of sera obtained from volunteer blood donors and patients with apparent non-a, non-b hepatitis and/or hepatitis b virus used in this elisa were compared with those of a commercially available first-generation c-100 elisa (using nonstructural hcv antigens), an experimental second-generatio ... | 1992 | 1374903 |
| hepatitis c virus infection in chronic haemodialysis patients, a clinicopathologic study. | fifty-two patients on regular haemodialysis at our institution were evaluated for the presence of hcv infection. evaluation included detailed history, clinical examination, and monthly screening for anti-hcv antibody, liver enzymes (alt, ast), serum iron and ferritin. also, three-monthly screening for other viral markers, hbv (hbsag, hbsab, hbcab), cmv (igg and igm), ebv, and hiv. anti-hcv antibody was found in 21 patients (40.4%). there was a significant (p less than 0.05) relationship between ... | 1992 | 1375712 |
| a universal t cell epitope-containing peptide from hepatitis b surface antigen can enhance antibody specific for hiv gp120. | peptide-based vaccines that directly target t cell or b cell epitopes may have significant advantages over conventional vaccines. further, synthetic chimeric peptides that combine strong t cell epitopes with poorly immunogenic, but immunodominant, b cell epitopes or strain-conserved b cell epitopes may be useful in eliciting antibody to such important regions. here we characterize a human t cell epitope analyzed in 54 individuals immunized with a hepatitis b virus surface ag vaccine. primary cul ... | 1992 | 1376346 |
| post-transplant recurrent hepatitis b viral liver disease. viral-burden, steatoviral, and fibroviral hepatitis b. | recurrence of hepatitis is a well-documented complication of hepatitis b liver disease, post-transplantation. it is well established also that the earliest hepatocellular change is the appearance of hepatitis b viral (hbv) markers and that the disease is rapidly progressive. in this article on 17 liver transplants in 16 hbv positive patients with long-term follow-ups (100-1234 days), the distinctive pathologic features of this disease are emphasized: the extreme viral load, the steatosis, and/or ... | 1992 | 1376555 |
| fine specificity of the human t-cell response to the hepatitis b virus pres1 antigen. | the t-cell response to hepatitis b virus envelope antigens was studied in 11 hepatitis b vaccine recipients; 7 were selected to analyze the fine specificity of the t-cell response to the pres1 antigen. four distinct t-cell epitopes were identified by peripheral blood lymphomononuclear cell stimulation with a panel of short synthetic peptides covering the pres1 sequence. the immunodominance of the pres1 epitopes included within peptides 21-30 and 29-48 was shown by their capacity to restimulate a ... | 1992 | 1377142 |
| analysis of antigenic polypeptides of dane particles and antibody response ability of hbv infected subjects to pres1 polypeptides. | we demonstrated the constitutive polypeptides (pp) of dane particles employing western blot and investigated the antibody response ability of hbv infected subjects to pres1 pp in comparison with other serum markers from hbv infected individuals. the results indicated that 1) the major reason for discrepant results may be related to the detergents used in the sample solutions and the degree of denaturation the samples had undergone; 2) there are 12 bands in the page-graph of dane particles. by we ... | 1992 | 1377756 |
| immune response of peripheral blood mononuclear cells to antigenic determinants within hepatitis b core antigen in hb virus-infected man. | residues 72-146 within hepatitis b core ag (hbcag) represent t-cell recognition site in hb-virus-infected man. this study was undertaken to define critical residues involved in the immunogenicity of dominant t-cell determinants of hbcag. for this purpose, p120-131 and its analog (p120-131 [a] containing alanine substitutions at residues 122 and 125, which were identified as epitopic residues in mice, were synthesized. these peptides and recombinant hbcag were analyzed for their ability to stimul ... | 1992 | 1377767 |
| [hepatitis serology: use and interpretation]. | presently five viruses causing hepatitis are known, the hepatitis viruses a (hav), b (hbv), c (hcv), d (hdv) and e (hev). the genomic structure is known of most of all these viruses as well as some of their structural and regulatory gene products. using radio- and enzyme immunoassays viral antigens can be detected for hbv and hdv as well as specific antibodies against all the five viruses. the results of these tests are the basis for the diagnosis and the follow-up of these infections but differ ... | 1992 | 1377839 |
| [interferon treatment of chronic viral hepatitis]. | the interferon treatment of chronic viral hepatitis leads to a measurable inhibition of replication of the various hepatitis viruses. in adult patients with chronic hbe-ag-positive hepatitis b, the seroconversion rate is tripled by therapy when compared with untreated controls. in chronic hepatitis c, biochemical remission is achieved in about 50% of patients, but there is a high tendency to relapse. interferon treatment has little effect in hepatitis b virus infection transmitted perinatally an ... | 1992 | 1377840 |
| the sequential change of serum 2',5' oligoadenylate synthetase in different infectious patterns of duck hepatitis b virus in ducks in experimental transmission. | duck hepatitis b virus (dhbv) shows clear age-dependent infectious patterns like that of hepatitis b virus, and many factors have been assumed to have a role in the persistence of the infection. in the present study, the activities of the interferon-induced enzyme 2',5' oligoadenylate synthetase (2,5as) were observed sequentially in the serum of ducks experimentally infected with dhbv on posthatch days 1, 7 and 14. these were compared with the infectious pattern to investigate whether the endoge ... | 1992 | 1378031 |
| serum pre-s1 and pre-s2 antigens as prognostic markers in interferon therapy for chronic hepatitis b. | we investigated the changes of serum pre-s1 and pre-s2 antigens before and after interferon therapy in 35 carriers with hbeag, to examine their clinical significance in the therapy. both antigens were measured quantitatively by solid-phase enzyme immunoassays using monoclonal antibodies specific to each antigen. the titers of these antigens of responders before therapy were significantly lower (p less than 0.001) than those of non-responders (6.23 +/- 1.09 versus 8.65 +/- 2.09 and 4.46 +/- 1.27 ... | 1992 | 1379373 |
| prevalence of anti-hcv in subjects of various age groups. | 812 serum samples from 382 males and 430 females from various age groups were examined. all the samples were tested for anti-hcv hepatitis c virus, anti-hbc and hbsag with an enzymeimmunoassay. the total serum prevalence was 2.9% for anti-hvc, 22.2% for anti-hbc and 4.6% for hbsag. the seropositivity rates of anti-hcv and anti-hbc tended to increase with age, while for hbsag a more regular pattern was observed for the different subject groups. the fact that anti-hcv are more frequently found tog ... | 1992 | 1379536 |
| expression of the amino-terminal half of the ns1 region of the hepatitis c virus genome and detection of an antibody to the expressed protein in patients with liver diseases. | a cdna fragment encompassing the 5'-terminal half of the ns1 region of the hepatitis c virus (hcv) genome was cloned. the cdna was expressed in insect cells using a recombinant baculovirus, and a protein band of approximately 21k was identified by immunoblotting with a serum sample from a patient with chronic hepatitis c. antibody to the protein was detected in sera from 13.4% of patients with chronic non-a, non-b hepatitis (nanbh), 20.8% of patients with liver cirrhosis and 16.8% of patients wi ... | 1992 | 1379627 |
| hepatitis-c-virus-related chronic liver disease of sporadic type: clinical, serological and histological features. | hepatitis c virus (hcv) is the most important cause of transfusion-related non-a, non-b hepatitis. it is also thought to be the prime cause of non-transfusion-related or sporadic chronic liver disease. to assess the extent of hcv infection and its significance in this last form, we evaluated the clinical, serological and histological features of 84 consecutive hcv-related patients without a history of blood or blood products transfusion, alcohol or intravenous drug abuse or other known risk fact ... | 1992 | 1379948 |
| enhancement of hepatitis-b surface-antigen expression by 5-azacytidine in a hepatitis-b-virus-transfected cell line. | the human hepatoblastoma-derived cell line hb611 secretes hepatitis-b surface antigen (hbsag) and hepatitis-b e antigen (hbeag) into the medium. hepatitis-b-virus (hbv) dna integrated into the cellular genome was found to be hypermethylated. when the cells were treated with 5-azacytidine for 3 days, the level of hbsag in the medium increased, while the level of hbeag remained constant. the level of alpha-fetoprotein (afp) decreased with the 5-azacytidine treatment. southern blot analysis of dna ... | 1992 | 1379994 |
| hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral rna genome. | hepadnaviruses, as well as other pararetroviruses, express their pol (p) gene product unfused to the preceding core gene implying that these retroelements have developed a mechanism for initiating assembly and replication that is principally different from the one used by retroviruses and retrotransposons. we have analysed this mechanism for the human hepatitis b virus by using a newly developed, highly sensitive detection method based upon radiolabelling of the p protein at newly introduced tar ... | 1992 | 1380455 |
| spontaneous exacerbation of disease activity in patients with chronic delta hepatitis infection: the role of hepatitis b, c or d? | forty-six patients with chronic hepatitis delta virus infection were followed between 6 and 116 mo (mean = 32.8 mo; median = 24 mo). nineteen patients (41%) demonstrated clinical courses with episodes of biochemical reactivation (alt levels greater than or equal to 10 times baseline values [group a]). twenty-seven patients (59%) had stable clinical courses without biochemical reactivation (group b). patients in group a were younger than those in group b (30.5 vs. 35.3 yr; p = 0.03), were less li ... | 1992 | 1380478 |
| hepatitis b and c viral infections in patients with hepatocellular carcinoma. | the prevalence of hepatitis b and c virus infections was studied in 70 patients diagnosed as having hepatocellular carcinoma. in addition to viral serological markers, serum hepatitis b virus dna and hepatitis c virus rna were determined with a nested polymerase chain reaction assay. twelve patients (17%) were hbsag positive, 26 (37%) had antibodies to hbs, hbc or both and 32 (46%) were negative for all hepatitis b virus serological markers. prevalence of the antibody to hepatitis c virus was 63 ... | 1992 | 1380480 |
| long-term follow-up of hepatitis b chronic carriers who responded to interferon therapy. | we studied the long-term outcome of patients with chronic hepatitis b virus (hbv) infection who responded to interferon (ifn) therapy. between 1983 and 1988, 120 patients were included in 5 different protocols; 94 patients were treated with ifn and 26 were controls. loss of serum hbv-dna was considered a partial response and occurred in 34 of the treated patients and in 10 of the controls. only the partial-response patients were followed up for 14-64 months (mean 46 months). hbeag disappeared in ... | 1992 | 1380526 |
| risk factors for dialysis-associated hepatitis c in venezuela. | utilizing the first and second generation of enzyme immunoassays which detect antibodies to the c virus we investigated the frequency of anti-hcv antibodies in 315 patients undergoing hemodialysis. other subpopulations at risk were used as reference groups. one hundred and twenty-three samples (39%) from the hemodialysis group repeatedly showed anti-hcv positive antibodies while only 19% and 1% were positive in the reference groups. the rate of anti-hcv reactive patients correlated with time on ... | 1992 | 1381002 |
| identification of a new group-specific determinant on hepatitis b surface antigen with a synthetic peptide. | in a recent study we demonstrated that a synthetic peptide representing residues 124-147 of the major protein of hepatitis b surface ag (hbsag) undergoes spontaneous oligomerization to reconstruct one or more conformational group-specific determinants on hbsag. the present study was undertaken to identify and characterize the hbsag-related antigenic determinants on this oligomeric peptide (peptide os[124-147]). a panel of nine analogs of this peptide was generated by either deleting, substitutin ... | 1992 | 1381396 |
| presentation of hepatitis b virus pres2 epitope on bluetongue virus core-like particles. | a chimeric protein containing most of the hepatitis b virus pres2 region (amino acid residues 1-48) upstream to, and colinear with the amino-terminus of bluetongue virus vp7 protein (pres2-vp7) was expressed by a recombinant autographa californica nuclear polyhedrosis virus (acnpv). the chimeric protein formed btv core-like particles (clps) in spodoptera frugiperda cells only when the cells were coinfected with this recombinant virus and a recombinant baculovirus that expresses unmodified vp7 an ... | 1992 | 1381538 |
| seropositivity to hepatitis c virus (hcv) in saudi children with chronic renal failure maintained on haemodialysis. | twenty saudi children (mean age: 7.7 years) with chronic renal failure who had received several blood transfusions were screened for antibodies to hepatitis c virus (anti-hcv), antibodies to the human immunodeficiency virus (anti-hiv) and antibodies to the various markers of hepatitis b virus (hbv). prevalence of anti-hcv antibodies was significantly higher in these patients (45%) than in controls (1%) (p less than 0.001). in contrast, the exposure rate to hbv was similar in both groups (15.0% i ... | 1992 | 1381899 |
| immunogenicity of recombinant core particles of hepatitis b virus containing epitopes of human immunodeficiency virus 1 core antigen. | a gag protein segment of human immunodeficiency virus 1 (hiv-1) has been fused to a c terminally truncated core antigen of hepatitis b virus (hbcag) using an e. coli expression system. fusion of 90 amino acids of hiv-1 gag protein to hbcag still allowed the formation of capsids presenting on their surface epitopes of hiv-1 core protein, whereas fusion of 317, 189, or 100 amino acids of gag prevented self-assembly of chimeric particles. mice immunized with recombinant particles emulsified with fr ... | 1992 | 1381912 |
| [liver transplantation and hepatitis b virus. some reasons to advance]. | 1992 | 1382029 | |
| [hepatitis c infection and liver cell carcinoma]. | epidemiological data disclose that the incidence of hepatocellular carcinoma (hcc) is increasing world-wide, whereas the number of cases positive for hbv-marker has remained almost stable, at least in japan. data from europe show positivity of antibodies against hepatitis c virus (anti-hcv) in 72% of hbsag negative cases with hcc and in 28% of patients positive for hbsag. nearly 90% of hbsag negative patients with hcc showed a histology of cirrhosis or chronic active hepatitis in the noncancerou ... | 1992 | 1382306 |
| relationship between fatty liver, alanine aminotransferase, hbsag and hepatitis c virus. | a community health survey of 923 residents aged 30 years or more was performed in putai township of taiwan. to elucidate the relationships between hepatitis c virus (hcv) and surrogate tests for non-a, non-b hepatitis in hyperendemic areas of hepatitis b virus (hbv) serum levels of alanine aminotransferase (alt), triglycerides, cholesterol, hepatitis b surface antigen (hbsag) and antibody to hcv (anti-hcv) were examined. glucose tolerance tests and the history of diabetes treatment were used to ... | 1992 | 1382655 |
| analysis of cost-effectiveness of different strategies for hepatocellular carcinoma screening in hepatitis b virus carriers. | a mathematical model was used to calculate the efficacy of screening to detect hepatocellular carcinoma at a resectable stage in hepatitis b virus carriers. data relating to tumour incidence, efficacy of screening tests and tumour growth times were obtained from a literature review. various tests were costed according to charges currently prevailing at the authors' institution. the cost per early tumour detected is inversely proportional to tumour incidence. it is relatively low for populations ... | 1992 | 1382657 |
| hepatitis c virus infection in chronic liver disease in bombay. | to find out the prevalence of antibody of hepatitis c virus (anti-hcv) in patients with chronic liver disease in bombay, sera from 126 patients (93 men, 33 women; aged 9-70 years, mean 39.7) with chronic liver disease (cirrhosis 103, cirrhosis with hepatocellular carcinoma 3, chronic active hepatitis 20) were tested for hbsag and anti-hcv antibody. hbsag positive sera were tested for anti-delta antibody and igm anti-hbc. all the tests were carried out by elisa. of 126 patients, 51 (40.5%) were h ... | 1992 | 1383141 |
| antibodies to hepatitis c virus among patients with hepatocellular carcinoma and blood donors in thailand. | hcc is the most cancer among thai men. it is not known if hcv plays an oncogenic role in hcc in this country where hbv is endemic. anti-hcv and hbsag were assayed in 154 sera from hcc and 3,387 voluntary blood donors. the prevalence of anti-hcv in hcc (8.4%) was significantly higher than blood donors (1.38%). the prevalence of hbsag in hcc (61%) was also significantly higher than blood donors (5.28%). the prevalence of anti-hcv in hcc was lower than that of spain, italy, africa and taiwan. anti- ... | 1992 | 1383370 |
| hepatitis b surface antigen particles of subtypes adw and adr, and compound subtype (adwr) in symptom-free carriers in japan. | of sera from 1,878 japanese blood donors who carried hepatitis b surface antigen (hbsag), 420 were subtyped as adw (22.4%) and 1,443 as adr (76.8%); only 15 (0.8%) contained hbsag of subtype ayw or ayr. sera with hbsag/adr had higher hbsag titres than those with hbsag/adw (geometric mean of haemagglutination titre: 10.1 +/- 2.4 vs. 9.7 +/- 2.4, p less than 0.01), and a higher prevalence of hepatitis b e antigen (24% vs. 13%, p less than 0.001). carriers of hbsag/adr progressively predominated ov ... | 1992 | 1383415 |
| persistence of hepatitis b virus dna after reduction of viral replication in serum and liver. | liver and serum samples from 67 children with hepatitis b chronic infection, whether or not treated with recombinant interferon, were analyzed for the presence of hepatitis b virus dna. after follow-up, 44/67 (66%) still had serum and liver viral dna; 23/67 (34%) were negative for serum hepatitis b virus dna. of the 23 children in the latter group, liver biopsy was available in 21 and viral dna was not detected by southern-blot in 20. in the remaining patient, viral dna was in an episomal nonrep ... | 1992 | 1383416 |
| effects of transforming growth factor-beta 1 against the inhibitory action of interferon on dna synthesis and viral replication in hepatitis b virus dna-transfected cell. | studies were undertaken to examine the effects of recombinant human transforming growth factor beta 1 (tgf-beta 1) on dna synthesis and antiviral actions of interferons (ifns) in hepg2 cell, a hepatoma cell line, transfected with hepatitis b virus (hbv) dna. the inhibitory effects of ifn-alpha and -gamma on dna synthesis of hepg2 cells were enhanced in a dose-dependent manner by a simultaneous addition of tgf-beta. the degree of suppression by the reagents was greater in hbv-nontransfected cells ... | 1992 | 1383417 |
| a role for hepatitis c virus infection in type ii cryoglobulinemia. | type ii cryoglobulinemia is a vasculitis characterized by cryoglobulins consisting of complexes of polyclonal igg and monoclonal igm rheumatoid factors. the cause of these immune complexes is unknown, though both the hepatitis b (hbv) and c (hcv) viruses have been suspected. | 1992 | 1383822 |
| hepatitis c core antibody detection in acute hepatitis and cirrhosis patients from tunisia. | the detection of anti-hepatitis c virus (hcv) core antibodies is an important addition to hcv antibody testing. in this study it appears to be more specific than the first generation hcv tests and in combination with the detection of anti-c33c antibodies, it is possibly more sensitive. in tunisia hepatitis c virus is implicated by the presence of anti-core antibodies in only 8% of the adult cases of acute hepatitis as opposed to 60% for hepatitis b virus (hbv) and 4% for hepatitis a virus (hav). ... | 1992 | 1383914 |
| mutations within the s gene of hepatitis b virus transmitted from mothers to babies immunized with hepatitis b immune globulin and vaccine. | a variant of hepatitis b virus (hbv) having a specific mutation within the s gene has been found to infect vaccinees. to know whether similar variants were involved in japan, we analyzed two cases of maternal transmission of hbv in infants immunized with hepatitis b immune globulin and hepatitis b vaccine. dna clones of hbv s genes were propagated from patients and family members and sequenced. in one family, the dna clones from the baby patient had a gly-to-arg mutation at the 145th codon of th ... | 1992 | 1383917 |
| seroepidemiologic study of hepatitis c virus in sexually transmitted disease risk groups. | to identify the importance of heterosexual activity as a possible route for the transmission of the hepatitis c virus (hcv), a screening of antibodies against hcv (anti-hcv) was performed in 200 sexually transmitted disease patients with different risks for incurring genital infections as well as in 100 registered prostitutes. out of all 300 persons tested, 14 cases of hcv infection were detected. anti-hcv was present in 3 of the prostitutes and in 11 of the std patients. evaluating known risk f ... | 1992 | 1384151 |
| toleration of amino acid substitutions within hepatitis b virus envelope protein epitopes established by peptide replacement set analysis. ii. region s(122-136). | the envelope of the hepatitis b virus (hbv) consists of three related proteins, designated s-, m- and l-protein, all of which share a common 226-amino acid residue sequence, corresponding to the s-protein that is sufficient for eliciting protective immunity against hbv. hbv variants, resulting from point mutations leading to replacements of amino acids within the s(122-160) segment of s-protein, have been recently recognized. in order to assure the continued success of vaccination against hbv an ... | 1992 | 1384823 |
| the reverse transcriptase of hepatitis b virus acts as a protein primer for viral dna synthesis. | hepatitis b viruses (hepadnaviruses) replicate their dna genomes by reverse transcription of an rna intermediate. efforts to examine the biochemical mechanism for viral dna synthesis have been hampered by the failure to solubilize the reverse transcriptase from virions and to express the polymerase in heterologous systems in an enzymatically active form. here, we demonstrate that the polymerase of a hepadnavirus synthesized in an in vitro translation reaction exhibits reverse transcriptase activ ... | 1992 | 1384989 |
| viral hepatitis in the 1990s, part iii: hepatitis c, hepatitis e, and other viruses. | acute hepatitis can be caused by a number of viruses, especially a, b, c, e, delta, epstein-barr virus, and cytomegalovirus. hepatitis a and b have been discussed previously in this series. the virus responsible for most cases of what commonly has been referred to as non-a non-b hepatitis has been tracked, and antibodies to certain proteins of this virus have been identified. this virus is now referred to as hepatitis c. the possible clinical outcomes after acute hepatitis c virus infection are ... | 1992 | 1385012 |
| role of hepatitis c virus infection in german patients with fulminant and subacute hepatic failure. | to investigate the possible role of hepatitis c virus (hcv) in fulminant and subacute liver failure, we tested serum and liver of 13 patients undergoing orthotopic liver transplantation for the presence of hcv rna. hcv rna was detected in specimens from two out of eight patients negative for all viral markers with suspected hepatitis non-a, non-b infection and in one out of four patients with hepatitis b virus infection. only in this patient replication of hcv could be demonstrated. we conclude, ... | 1992 | 1385150 |
| serum and liver hepatitis b virus dna in chronic hepatitis b after sustained loss of surface antigen. | polymerase chain reaction (pcr) was used to detect hepatitis b virus dna in the sera and livers of nine patients with chronic hepatitis b after treatment-induced or spontaneous loss of serum hepatitis b surface antigen. patients were evaluated at intervals ranging from 3 to 67 months after disappearance of hepatitis b surface antigen. pcr was performed using primer pairs from the surface and core gene regions, and surface gene products were quantitated. liver tissue was also evaluated by in situ ... | 1992 | 1385254 |
| prevalence of antibodies to hepatitis c virus among saudi patients with chronic liver diseases. | the prevalence of antibodies against hepatitis c virus (anti-hcv) was determined in 55 patients with chronic liver diseases including liver cirrhosis (42 patients), liver cirrhosis and hepatocellular carcinoma (8 patients), and chronic active hepatitis (4 patients). a total of 63.6% of these patients were positive for anti-hcv, a significantly higher prevalence than the rate of 3.9% observed in 488 asymptomatic volunteers. of the 42 patients with liver cirrhosis 16 (38.1%) had positive anti-hcv ... | 1992 | 1385286 |
| the antiviral effects of the interferons and their inhibition. | 1992 | 1385551 | |
| immunisation of children born to mothers positive for anti-hbe. | 1992 | 1385744 | |
| efficacy of hepatitis b vaccination in primary school children from a village endemic for schistosoma mansoni. | to determine whether chronic schistosoma mansoni infection interferes with hepatitis b virus (hbv) immunization, 308 schoolchildren aged 6-12 years with no evidence of prior hbv infection (156 with active schistosomiasis) were vaccinated with three 5-micrograms injections of recombinant dna-derived hbv vaccine. the vaccine was given in the deltoid muscle at time 0 and 1 and 7 months later. all vaccinees were examined 1 and 3 years after vaccination for quantitative antibody to hepatitis b surfac ... | 1992 | 1386097 |
| microbiology, infection control, immunizations, and infectious disease exposure: education and practices in united states nursing schools. | previous data suggest that nursing students in the united states are inadequately protected against hepatitis b. this survey focused on the immunization and education practices, infection control knowledge, and follow-up to infectious disease exposure by u.s. nursing schools. | 1992 | 1386200 |
| hepatitis b virus infection among swedish adults: aspects on seroepidemiology, transmission, and vaccine response. | 1992 | 1386474 |