Publications

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[localization and identification of adhemiba i, a protein which participates in the adhesion of entamoeba histolytica to human erythrocytes and epithelial cells]. 19862884949
adhesion of entamoeba histolytica trophozoites to human erythrocytes. 1. maintenance of the integrity, viability and adhesion of trophozoites in different media. 19862884950
adhesion of entamoeba histolytica trophozoites to human erythrocytes. 3. the inhibition patterns by carbohydrates are strain-specific. 19862884951
[characterization of 3 mutants of entamoeba histolytica deficient in virulence with different phenotypes for adhesion and toxin activity]. 19862884952
[mitogenic and lectin activity of mutants of entamoeba histolytica deficient in virulence]. 19862884953
[multifactorial mechanism for the cytolytic activity of entamoeba histolytica]. 19862884954
cytopathogenicity of entamoeba histolytica; a cytoplasmic heat stable factor stimulates dna synthesis in mammalian cells. 19862884955
alteration of isoenzyme patterns of a cloned culture of non pathogenic entamoeba histolytica upon changes in growth conditions. 19862884956
anti-surface antibodies to entamoeba histolytica and their role in complement lysis. 19862884957
[acid phosphatase activity of 2 strains of entamoeba histolytica of different virulence]. 19862884958
[diapedesis in experimental amebiasis]. 19862884959
[experimental amebiasis in the guinea pig: a model for resistance]. 19862884960
[immunogenicity of a delipidated antigen of entamoeba histolytica]. 19862884961
[inhibition by antibodies of the adhesion of trophozoites of entamoeba histolytica]. 19862884962
in vitro studies on the interaction of human monocytes and the monocyte locomotion inhibitory factor produced by e. histolytica. 19862884963
[ultrastructural changes associated with the inhibition of human monocyte chemotaxis caused by products of e. histolytica cultured in axenic medium]. 19862884964
[effect of supernatants obtained from lymphocytes stimulated with concanavalin a on trophozoites of entamoeba histolytica]. 19862884965
[effect of cellular immunity on the interaction between the peritoneal cell and the ameba in vitro]. 19862884966
[induction of a local humoral immune response against antigens of entamoeba histolytica]. 19862884967
[detection of class iga antibodies directed against a lipopeptidophosphoglycan of e. histolytica in samples of human colostrum]. 19862884968
[interaction in vitro of trophozoites of entamoeba histolytica with macrophages from human colostrum]. 19862884969
[genetic transformation in entamoeba histolytica]. 19862884970
[evaluation of cellular immunity in patients with amebic liver abscess using the leukocyte migration inhibition test]. 19862884971
[clinical correlation of the zymodeme of entamoeba histolytica in patients in a psychiatric hospital]. 19862884972
epidemiology of amebiasis in a rural community of mexico: serologic and coproparasitoscopic survey. 19862884973
[cell-cycle phases of entamoeba histolytica]. 19862884974
[epidemiology and control of amebiasis in mexico]. 19862884975
amebiasis in the world. 19862884976
[comparative study of zymodemes of entamoeba histolytica and the genus entamoeba in polyacrylamide gels]. 19862884977
[production of e. histolytica hm-1 in agitated axenic culture and by fermenter]. 19862884978
[purification of the polysaccharide portion of the lipopeptidophosphoglycan extracted from trophozoites of entamoeba histolytica]. 19862884979
[characterization of the ribosomal proteins of entamoeba histolytica]. 19862884980
[motility mutants of entamoeba histolytica resistant to cytochalasin d]. 19862884981
[phases of nuclear division in entamoeba histolytica]. 19862884982
modification of the ultrastructure of entamoeba histolytica after phagocytosis of liposomes loaded with phalloidin.the specific actin-interacting drug phalloidin has been introduced into the cytoplasm of a highly motile amoeba, entamoeba histolytica, by a new technique: the phagocytosis of liposomes containing phalloidin. after ingestion of these liposomes, two important modifications of the ultrastructure of the amoeba were observed. first, large nodules of densely packed fine filaments are formed, which may be due to the polymerization of actin induced by the release of phalloidin within the cell's cytopla ...19872885196
inhibition of growth of entamoeba histolytica by allicin, the active principle of garlic extract (allium sativum). 19872885381
use of monoclonal antibodies to identify, characterize, and purify a 96,000-dalton surface antigen of pathogenic entamoeba histolytica.we identified and partially characterized a surface antigen of entamoeba histolytica by using seven monoclonal antibodies obtained after injecting mice with a pathogenic strain of amoeba. an intrinsically radiolabeled 96,000-dalton antigen was immunoprecipitated by five of the seven monoclonal antibodies; this antigen was present in three strains of e. histolytica. the antigen was situated on the external surface of e. histolytica, as demonstrated by agglutination and immunofluorescence staining ...19872885384
ultrastructural changes associated with the inhibition of monocyte chemotaxis caused by products of axenically grown entamoeba histolytica.the supernatant fluid of axenically grown entamoeba histolytica-hm1 significantly modifies the ultrastructural features associated with monocyte chemotaxis as assayed in boyden chambers. this morphological evidence supports the existence of a factor, monocyte locomotion inhibitory factor (mlif), produced by e. histolytica that inhibits the in vitro locomotion of human monocytes. none of the leucocyte-locomotion modifying drugs included in this study (i.e., cytochalasin-b, colchicine, vinblastine ...19872885445
ameba-bacterium relationship in amebiasis. 19872885733
[is this ameba dangerous?]. 19872885764
in vitro amoebicidal testing of natural products; part 2. alkaloids related to emetine. 19872885873
a dot immunobinding assay (dia) on nitrocellulose membrane for the serological detection of antibodies to entamoeba histolytica.a rapid, cheap, simple and specific serological test of adequate sensitivity for detecting igg antibodies against entamoeba histolytica antigen is described. axenically cultured amoebic antigen was used to precoat the nitrocellulose membrane. the strips were incubated with test samples and later with horseradish peroxidase (hrpo) labelled protein-a conjugate. a dark blue spot was obtained by treatment with peroxidase substrate, 4-chloro-1-naphthol, in positive samples. serum samples from 32 heal ...19862885949
seroepidemiology of e. histolytica infection in hyderabad. 19862885950
seroepidemiologic study of giardiasis patients and high-risk groups in a midwestern city in the united states.serum antibodies to giardia lamblia were measured in giardiasis patients, in groups at high risk for intestinal parasite infection, and in controls by an indirect fluorescent antibody (ifa) technique. symptomatic patients had the highest antibody titers, and antibodies remained present for up to 18 months in persons with chronic infection. indochinese refugees and male homosexuals with the acquired immunodeficiency syndrome (aids) and pre-aids had higher mean antibody levels than did healthy con ...19872886069
the role of gamma interferon in the generation of human macrophages cytotoxic for entamoeba histolytica trophozoites.upon exposure to entamoeba histolytica antigen, lymphocytes from patients treated for amebic liver abscess produce lymphokines which activate monocyte-derived macrophages to kill e. histolytica trophozoites. we now demonstrate that gamma interferon (ifn-gamma) is produced by these stimulated lymphocytes and is sufficient but not exclusively necessary to activate monocyte-derived macrophage amebicidal activity. supernatants from mononuclear cells of 7 patients when stimulated with amebic antigen ...19872886071
evidence for two mechanisms of human erythrocyte endocytosis by entamoeba histolytica-like amoebae (laredo strain).a study of the ultrastructural aspects of endocytosis of human erythrocytes by entamoeba histolytica-like amoebae (laredo) revealed two different mechanisms of endocytosis. first, there is classical phagocytosis which consists of the formation of a phagocytotic pseudopod. this process begins with the engulfment of the red blood cell followed by its entrapment in a food vacuole of the same size as the erythrocyte. it is then digested in the food vacuole. the second means of endocytosis is achieve ...19872886165
entamoeba histolytica: chemoattractant activity for gerbil neutrophils in vivo and in vitro.the kinetics of the host's cellular response in the peritoneal cavity of gerbils toward axenic pathogenic and nonpathogenic entamoeba histolytica strains were examined. amebae contained in diffusion chambers or free in the peritoneum elicited a neutrophilic response accompanied by decreased levels of macrophages and lymphocytes. pathogenic amebae (ip:0682:1 strain) elicited a neutrophilic response greater than the nonpathogenic dkb and "entamoeba-like" laredo amebae. the neutrophil eliciting fac ...19872886359
cytopathogenicity of entamoeba histolytica: the role of amebic adherence and contact-dependent cytolysis in pathogenesis.the mechanisms by which entamoeba histolytica trophozoites adhere to and lyse target cells are reviewed from the perspective of pathogenesis. adherence via the galactose and n-acetyl-galactosamine inhibitable amebic lectin and possible additional amebic adhesin molecules is followed by target cell death. inhibition of the gal/galnac lectin with galnac inhibits amebic cytolysis of target cells. amebic activities implicated in the cytolytic event include vesicle exocytosis and maintenance of an ac ...19872886360
[synthesis of new diheterocyclic derivatives of imidazole with antiparasitic activity].a description is given of potential cyclic imidazoles such as 3,3-dichloro-2-methylenimino-acrylonitrile derived from nitro or non-nitro heterocycles, as well as the hydrazone, semicarbazone and arylpropenone derivatives of the 4-chloro-2-(5-nitro-2-thienyl) (v), 5-imidazole carboxaldehyde (iv) and the 5-bromothienyl analogue. the antiparasitic activity was tested in vitro against trichomonas vaginalis and entameba histolytica. some of the nitro containing compounds showed trichomonacidal activi ...19872886364
stimulation by target cell membrane lipid of actin polymerization and phagocytosis by entamoeba histolytica.the identity of molecules of mammalian target cells that stimulate contact-dependent attack by entamoeba histolytica was sought using human erythrocytes (rbc) as a model. protein-free liposomes prepared from rbc membrane lipids stimulated the same rapid e. histolytica actin polymerization and phagocytosis as did whole target cells. liposomes constructed from the major phospholipids of rbc stimulated these responses but only if a negatively charged phospholipid was included. the addition to these ...19872886434
enzyme linked protein-a: an elisa for detection of amoebic antibody.enzyme linked protein-a was used to develop an enzyme linked immunosorbent assay (elisa) system for the detection of circulating antibodies to amoebic antigen. the specificity of protein-a to bind igg only through fc receptors, makes the test more specific for the detection of igg antibodies to amoebic antigen. the elisa system was used to detect amoebic antibody in control subjects (56), patients with amoebic liver abscess (79) and entamoeba histolytica cyst-passers (10) and the results compare ...19872887049
physiochemical alteration of surface membrane of axenic entamoeba histolytica during repeated cholesterol passage. 19872887508
elicitation of protective immunity to entamoeba histolytica--an experimental study.immunization of hamsters with plasma membrane (pm) antigens of virulent subline of axenic entamoeba histolytica (nih: 200 v) entrapped in multilamellar phosphatidyl choline liposomes conferred 100% protection to a subsequent intrahepatic amoebic challenge. in contrast, vaccination of hamsters with live amoebic trophozoites injected intradermally failed to protect any of the animals. the protected animals had significantly high levels of anti-pm anti-amoebic antibodies, cellular sensitization and ...19872887509
blastocystis hominis and human disease. 19872887585
vaccination against experimental hepatic amoebic infection--an evaluation of phosphatidylcholine liposomes as immunopotentiator. 19872887647
entamoeba histolytica: cloning and characterization of actin cdna.in order to study gene expression in the human parasite entamoeba histolytica, a cdna library of e. histolytica strain 200:nih was constructed using the phage vector lambda gt10. three cdna clones (a, b and c) were selected for further analysis. each of the three clones hybridized to a distinct mrna. two of these mrnas were translated in vitro after hybrid selection, and yielded distinct translation products. one of these mrnas, selected by hybridization to clone a, encodes the most abundantly e ...19872888016
[amebiasis: recent progress]. 19872888181
a double-antibody sandwich elisa for the detection of entamoeba histolytica antigen in stool samples of humans.a double-antibody sandwich elisa was developed to detect detergent-solubilized antigens of entamoeba histolytica in stool samples of humans. the test system was evaluated for its methodical and diagnostic sensitivity and specificity. in recovery experiments the lower limit of detection was 400 ng e. histolytica (hk9) protein/ml stool, corresponding to approximately 2000 amoebic trophozoites/ml stool. samples of 97 patients with suspected intestinal amoebiasis were examined. specific antigens wer ...19872888183
[effect of the biotope and the intensity of invasion on the activity of various oxidation-reduction enzymes in entamoeba histolytica]. 19872888240
[synthesis and antiparasitic activity of new nitro-5-imidazole derivatives].syntheses are given for derivatives of the type: 2-amino-1-[(1-methyl-5-nitro-2-imidazolyl) methyleneimino] imidazole, of new hydrazones of 1-methyl-5-nitroimidazole-2-carboxaldehyde and also for semicarbazones and a hydrazone of 1-methyl-5-nitro-2-(4-formyl-styryl)imidazole.19872888689
recognition of the galactose- or n-acetylgalactosamine-binding lectin of entamoeba histolytica by human immune sera.cure of amebic liver abscess is associated with resistance to recurrent invasive amebiasis and the development of a humoral and cell-mediated immune response. we determined whether human immune sera contain blocking antibody for the 170-kilodalton (kda) galactose or n-acetylgalactosamine (gal/galnac)-binding lectin of entamoeba histolytica. by western blot (immunoblot) of whole amebae subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all eight immune sera studied here promi ...19872888730
complement activation in patients with amebic liver abscess.serum or plasma concentrations of components of the classical (c1q, c4) and alternative (c3, factor b) pathways, regulatory protein factor h, and one of the c3 products of degradation, c3d, were determined in 19 patients with amebic liver abscess (ala). patients were divided into two groups. thirteen patients that recovered under medical treatment who had a significantly shorter clinical course on admission (p less than 0.05) (group 1) exhibited either normal (c1q; c4; factor b; c3d) or increase ...19872888804
isolation of a 220-kilodalton protein with lectin properties from a virulent strain of entamoeba histolytica.a 220-kilodalton (kda) protein with lectin properties was isolated from entamoeba histolytica strain hm1:imss and was purified by sepharose 4b chromatography and electroelution from 5% sds-polyacrylamide gels. the protein contains 9% carbohydrate by weight; is rich in hydrophobic residues; and is very immunogenic in mice, hamsters, and rabbits. the protein binds to fixed monolayers of mdck cells and inhibits trophozoite attachment to the cultured cells. the 220-kda protein agglutinates human ery ...19872888825
use of antibodies to characterize a 220-kilodalton surface protein from entamoeba histolytica.antibodies were prepared against a 220-kilodalton (kda) protein partially purified by sepharose 4b chromatography of entamoeba histolytica strain hm38:imss homogenates, and the protein was found to have lectin properties. the antibodies specifically recognized this protein in trophozoite homogenates. immunologically related molecules with the same molecular weight were identified by polyclonal antibodies in strains hm1:imss, entamoeba invadens, and entamoeba histolytica laredo. six monoclonal an ...19872888826
unloaded polyisobutylcyanoacrylate nanoparticles: efficiency against bloodstream trypanosomes.the potential use of polyisobutylcyanoacrylate nanoparticles in antiparasitic chemotherapy is described. the nanoparticles on their own proved to have trypanocidal activity against trypanosoma brucei brucei in-vitro but not against trichomonas vaginalis or entamoeba histolytica. the trypanocidal activity was partly confirmed in-vivo with t. brucei-infected mice.19872888859
a fast electrophoretic isoenzyme technique for the identification of invasive and non-invasive entamoeba histolytica and "e. histolytica-like" organisms.cellulose acetate electrophoresis (cae) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive entamoeba histolytica and "e. histolytica-like" organisms. each of these morphologically similar organisms possessed a unique cae isoenzyme profile that can be used as an aid in their identification. the cae technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither speci ...19872888886
in vitro evaluation of the activity of entamoeba histolytica studies on motility.the tube migration test for leukocyte function studies has been modified to test the motility of entamoeba histolytica. the test was more sensitive to the effect of antiamebic drugs than was a test for phagocytic activity. a 50% reduction in amebic motility was seen instantly after exposure to metronidazole (40 mg/l). a similar reduction of phagocytosis at the same concentration of the drug was seen only after two hours' preexposure. the extent of amebic migration in the test tube is dependent o ...19872889170
immunological studies in amebiasis: isotypic characterization of specific antibodies by enzyme-linked immunofiltration assay.fifty sera from 17 cases of invasive amebiasis (15 hepatic localizations, 1 ameboma, 1 diffused colic localization) were studied by enzyme-linked immunofiltration assay (elifa). igm and ige anti-entamoeba histolytica were detected in 10 and 15, respectively, of the 17 patients studied. igm and ige antibodies were found simultaneously in 10 cases; these 2 isotypes were only recognized twice in the same serum by the same antigenic components. during post-therapy monitoring, in less than 90 days ig ...19872889384
the electrophoretic isoenzyme patterns of strains of entamoeba histolytica isolated in two major cities in canada.the isoenzyme patterns of 92 isolates of entamoeba histolytica from british columbia and 28 from ontario were determined. seropositivity for e. histolytica was assessed by indirect hemagglutination and enzyme-linked immunosorbent assay in the one center and by elisa and amebic gel diffusion in the other. in both british columbia and ontario nonpathogenic zymodemes i and iii were most common. a newly described isoenzyme pattern was identified in ontario. only 9 of 120 zymodeme patterns identified ...19872889385
cholesterol induced changes in glucose-6-phosphate generating enzymes, concanavalin a agglutinability and haemolytic activity of axenic entamoeba histolytica.repeated passage of the 200-nih strain of entamoeba histolytica through cholesterol-enriched axenic growth medium induced marked increases in cholesterol, phosphoglucomutase and hexokinase levels and a less prominent rise in the protein content of amoebic cells. there was also pronounced enhancement of haemolytic activity and concanavalin a (con a) agglutinability of the culture, but no significant change was observed in glucose phosphate isomerase. these cholesterol-induced effects persisted to ...19872889429
a simplified in vitro assay correlating amebicidal activity with chelation by 8-hydroxyquinoline and related compounds. 19872889432
interaction of pathogenic and nonpathogenic entamoeba histolytica with human complement. 19872889433
isoenzyme patterns of strains of entamoeba histolytica isolated in british columbia. 19872889434
the reliability of entamoeba histolytica zymodemes in clinical laboratory diagnosis. 19872889435
specificity of a cysteine proteinase of entamoeba histolytica towards the alpha 1-cb2 peptide of bovine collagen type i.the cysteine proteinase of entamoeba histolytica was shown to hydrolyze the cyanogen bromide peptide alpha 1-cb2 of calf skin collagen type i yielding two split products. amino acid analyses of the formed peptides and estimation of the amino-terminal residues revealed that the alpha 1-cb2 peptide was exclusively cleaved between gly10 and leu11 within the sequence -arg9-gly10-leu11-yielding two peptides with 7 and 29 amino acids, respectively. under identical conditions the ratio of hydrolysis of ...19872890103
isolation and partial characterization of the hexokinase isoenzymes from pathogenic and non-pathogenic strains of entamoeba histolytica.isoenzyme electrophoretic patterns (zymodemes) are increasingly used to distinguish between pathogenic and non-pathogenic strains of entamoeba histolytica. isolates of e. histolytica from asymptomatic and symptomatic cases have been shown to differ in the electrophoretic mobility of their hexokinase and phosphoglucomutase isoenzymes. the hexokinase isoenzymes from a non-pathogenic strain and from a pathogenic strain of e. histolytica were purified by fast protein liquid chromatography in several ...19872890104
[confirmation of the identity of intestinal amoebae and evaluation of the virulence of entamoeba histolytica by isoenzyme electrophoresis]. 19872890195
alterations of humoral, cell mediated and antibody dependent cell mediated cytotoxic responses during the course of amoebic infection in guinea pigs.the cellular and antibody dependent cellular cytotoxic (adcc) responses of splenic lymphocytes and peritoneal macrophages obtained from animals at variable intervals after inoculation were studied against trophozoites of axenic e histolytica (nih:200). cytotoxic responses of effector cells from infected animals were compared with those of effector cells from vaccine stimulated and unstimulated uninfected control animals. cellular and antibody-dependent cellular cytotoxic responses of the effecto ...19872890558
isolation of the galactose-binding lectin that mediates the in vitro adherence of entamoeba histolytica.entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (gal) or n-acetyl-d-galactosamine (galnac) inhibitable surface lectin. blockade of this adherence lectin with gal or galnac in vitro prevents amebic killing of target cells. we have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared wi ...19872890654
rat and human colonic mucins bind to and inhibit adherence lectin of entamoeba histolytica.establishment of adherence by entamoeba histolytica is mediated by a 170-kd gal/galnac inhibitable lectin and is required for cytolysis and phagocytosis of mammalian target cells. we studied the biochemical mechanisms of the in vitro interaction between rat and human colonic mucins and axenic e. histolytica trophozoites. crude mucus prevented amebic adherence to chinese hamster ovary (cho) cells by up to 70%. purification of the colonic mucins by sepharose 4b chromatography, nuclease digestion, ...19872890655
entamoeba histolytica cyst passers--to treat or not to treat? 19872891196
a longitudinal study of asymptomatic carriers of pathogenic zymodemes of entamoeba histolytica.the value of treating subjects who pass entamoeba histolytica cysts in their faeces is currently in question. in the endemic durban area iso-enzyme electrophoresis of e. histolytica isolates indicated that 1% of the asymptomatic population are infected with pathogenic zymodemes. the outcome of these potentially invasive infections was established by means of a longitudinal survey. all subjects had strongly positive serological responses--10% of them developed amoebic colitis while the rest remai ...19872891197
cellular and humoral mechanisms of cytotoxicity: structural and functional analogies. 19872891261
longitudinal study on the health status of children in a rural tanzanian community: parasitoses and nutrition following control measures against intestinal parasites.three repeated cross-sectional surveys were undertaken among children (1 month to 15 years) of a rural community in southeastern tanzania. the study was part of a longitudinal project on the interactions among nutrition, parasitic infections and immunity within a primary health care programme emphasizing village health workers. all children underwent interviews and parasitological, anthropometric, anamnestic and clinical examinations. out of 550-590 children examined each year, a cohort of 170 c ...19872891267
immunoprotective behaviour of plasma-membrane-associated antigens of axenic entamoeba histolytica.immunisation of golden hamsters with plasma-membrane-associated antigens of a virulent subline of axenic entamoeba histolytica strain nih 200 v, entrapped in multilamellar phosphatidylcholine liposomes (mpl) or freund's complete adjuvant (fca), afforded protection against intrahepatic challenge with axenic amoebic trophozoites of the same strain. amoebic liver abscess developed in 86% and 80% of the animals that received empty liposomes or buffer emulsified in fca but in none of the animals that ...19872891855
iga protease in entamoeba histolytica trophozoites. 19872892356
antiamoebic properties of human colostrum. 19872892357
[entamoeba histolytica surface antigens that interact with specific human iga immune sera]. 19872892474
ova, larvae and cysts in fingernail contents. 19882892676
[pseudomembranous colitis after antibiotic therapy associated with the presence of entamoeba histolytica histolytica. apropos of 2 cases].the authors report on two cases of pseudomembranous colitis (p.m.c.) developed in two senegalese women of 38 and 36 years, and discovered at the 4th and 5th day respectively of an antibiotherapy based on ampicillin. in these two observations, cysts of entamoeba histolytica histolytica were found in both feces and biopsies. they recall the circumstances of the occurrence, diagnosis techniques and treatment. they underline the unfrequency of this disease in africa south of sahara and they discuss ...19872893239
catalytic classes of proteinases of entamoeba histolytica.endopeptidase inhibitors were used to determine the catalytic classes of proteinases present in extracts of entamoeba histolytica (strain hm 1:imss) axenically grown in vitro. cysteine proteinases account for most of the proteolytic activity; one or more proteinases with different catalytic mechanisms are also present but could not be unambiguously assigned to a particular catalytic class. proteinases in amebic lysates were resolved by polyacrylamide gel electrophoresis with sodium dodecyl sulfa ...19872893258
hla antigens associated to amoebic abscess of the liver in mexican mestizos.worldwide prevalence of amoebiasis is estimated at 4 x 10(8) cases/year, yet only one of about 300 individuals harbouring entamoeba histolytica suffers tissue invasion and these cases are mostly concentrated in certain areas of asia, africa and latin america. patients with amoebic abscess of the liver (aal) represent only a small fraction of that. these contrasting figures have been tentatively explained on the one hand through variations in sex, immunocompetence, nutritional and other socioecon ...19872893327
amoebic peritonitis in pregnancy in the united kingdom.we describe a woman who developed hepatic amoebic abscesses during pregnancy, and subsequently amoebic peritonitis. we believe this to be the first such recorded case in the united kingdom, and unusual in that the patient had never been abroad.19872893366
molecular weight analysis of entamoeba histolytica antigens recognized by igg and igm antibodies in the sera of patients with amoebiasis.the sera of four patients with amoebic liver abscess and two patients with diarrhea caused by entamoeba histolytica were tested on "western blots" prepared from e. histolytica strains a3, sfl-3 and hk-9 separated by sds-page. igg antibodies in the sera from patients with liver abscess were found to react with several "major" (i.e. strongly staining) band of approximate molecular weights 150-250 kd, 100 kd, 80 kd, 60 kd, 35 kd, 30 kd, 20 kd, and 5-15 kd and the 150-250 kd group of bands. a compar ...19872893443
course of entamoeba histolytica infection in nude mice.the course of intestinal infection by entamoeba histolytica was examined in nu/nu balb/c mice. mice were inoculated intracecally with 2.5 x 10(5) polyxenic trophozoites and killed at various times after injection. there was no significant difference in the number of e. histolytica trophozoites recovered in the ceca of nu/+ and t cell-deficient nu/nu mice for the first 25 days after amebic injection. thirty days following amebic injection the number of trophozoites recovered in the ceca of nu/nu ...19872893444
antigens of entamoeba histolytica recognized by immune sera from liver abscess patients.immune sera from 11 patients cured of amebic liver abscess was used to identify antigens of entamoeba histolytica. strain hm1-imss, among the most virulent in axenic culture, was used. the 37 and 90 kd antigens were surface glycoproteins as indicated by lactoperoxidase iodination and by concanavalin a blotting; the 59 kd antigen was a mannose containing glycoprotein that did not appear to be on the cell surface. western blots of 11 different immune sera revealed specific binding of immune igg to ...19882893553
appearance of sialoglycoproteins in encysting cells of entamoeba histolytica.amoeba-bacterium cultures of entamoeba histolytica transferred to a hypoosmotic medium depleted of nutrients changed morphologically and biochemically. the cells ejected grains of rice starch, rounded up, and formed a distinct cell wall that was resistant to detergent, bound the sialic acid-specific lectin from limulus polyphemus, and became fluorescent with calcofluor m2r. a subpopulation of these cells displayed more than one nucleus. all these signs are characteristic of encysting cells and w ...19882893775
amebic liver abscess in a european patient: zymodeme classification of entamoeba histolytica.this is a case report of a 36-year-old patient who developed an amebic liver abscess after a stay in the sudan. he was first misdiagnosed as having pneumonia of the right lower lobe. following establishment of the correct diagnosis, the patient recovered fully after metronidazole treatment. the fecal culture in robinson's medium yielded extensive growth of entamoeba histolytica. electrophoretic characterization proved it to be a zymodeme xix, which is one of the zymodemes associated with pathoge ...19882893858
comparison of repeated dna from strains of entamoeba histolytica and other entamoeba.restriction enzyme digestion patterns of total genomic dna revealed the presence of highly repeated dna in entamoeba histolytica and e. histolytica-like (laredo type) amoebae of humans, e. invadens and e. invadens and e. terrapinae of reptiles, and e. moshkovskii isolated from sewage polluted waters. homology with plasmodium berghei ribosomal dna was striking. northern blot analysis of e. histolytica rna provided further evidence that the highly repeated dna codes for ribosomal rna. the distribu ...19882893976
inhibition of the adhesion of entamoeba histolytica trophozoites to human erythrocytes by carbohydrates.we have analyzed the effect of 14 carbohydrates (seven monosaccharides, four disaccharides and three aminosugars) on the adhesion of entamoeba histolytica hk9 trophozoites to human red blood cells (rbc). amebal adhesion was significantly inhibited by five of these carbohydrates with the following order of potency: lactose (lac) greater than n-acetylgalactosamine (galnac) greater than melibiose (mel) greater than galactose (gal) greater than n-acetylglucosamine (glcnac). the mean inhibitory conce ...19872894024
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