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efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports.different technologies may be used for decolorization of wastewater containing dyes. among them, biological processes are the most promising because they seem to be environmentally safe. the aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (pleurotus ostreatus - bwph, gleophyllum odoratum - dca and polyporus picipes - rwp17). different solid supports were teste ...201729129408
development of novel polymorphic est-ssr markers in bailinggu (pleurotus tuoliensis) for crossbreeding.identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of pleurotus tuoliensis (bailinggu). however, conventional crossbreeding methods are troublesome and time consuming. using rna-seq technology, we developed new expressed sequence tag-simple sequence repeat (est-ssr) markers for bailinggu to easily and rapidly identify monokaryons and their mating types, genetic diversity and hybrid offspring. we identified 1110 potentia ...201729149037
differential expression patterns of pleurotus ostreatus catalase genes during developmental stages and under heat stress.catalases are ubiquitous hydrogen peroxide-detoxifying enzymes. they participate in fungal growth and development, such as mycelial growth and cellular differentiation, and in protecting fungi from oxidative damage under stressful conditions. to investigate the potential functions of catalases in pleurotus ostreatus, we obtained two catalase genes from a draft genome sequence of p. ostreatus, and cloned and characterized them (po-cat1 and po-cat2). po-cat1 (group ii) and po-cat2 (group iii) enco ...201729160795
establishment of an efficient transformation system for pleurotus ostreatus.pleurotus ostreatus is widely cultivated worldwide, but the lack of an efficient transformation system regarding its use restricts its genetic research. the present study developed an improved and efficient agrobacterium tumefaciens-mediated transformation method in p. ostreatus. four parameters were optimized to obtain the most efficient transformation method. the strain lba4404 was the most suitable for the transformation of p. ostreatus. a bacteria-to-protoplast ratio of 100:1, an acetosyring ...201729164387
a polysaccharide fraction extracted from pleurotus ostreatus mycelial biomass inhibit sarcoma 180 tumor.fungi of pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. these properties are attributed mainly to polysaccharides synthesized by pleurotus. this work aimed to study the mycelial growth of p. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/l of glucose) and the ph (4 and 6) on kinetic parameters of production of biomass. the effectiveness of di ...201729166531
laccase fermentation of clove extract increases content of dehydrodieugenol, which has neuroprotective activity against glutamate toxicity in ht22 cells.enzyme fermentation is a type of food processing technique generally used to improve the biological activities of food and herbal medicines. in this study, the syzygium aromaticum (clove) extract was fermented using laccase derived from trametes versicolor (ltv). the fermented clove extract showed greater neuroprotective effects against glutamate toxicity on ht22 than the non-fermented extract did. hplc analysis revealed that eugenol (1) and dehydrodieugenol (2) contents had decreased and increa ...201729169218
immunomodulatory effects of extracellular β-glucan isolated from the king oyster mushroom pleurotus eryngii (agaricomycetes) and its sulfated form on signaling molecules involved in innate immunity.the aim of this study was to determine, using murine raw 264.7 macrophages, the immunomodulatory effect of extracellular β-glucan isolated from pleurotus eryngii (pebg) and its sulfated derivative (pebg-s) on signaling molecules implicated in host innate immunity. β-glucan was extracted and purified from the mycelial culture using optimal medium concentrations. it was then chemically converted to its sulfated form. monosaccharide composition of β-glucan was characterized with p-aminobenzoic acid ...201729199562
effect of fungal species on the development and reproductive traits of the fungal-feeding mite rhizoglyphus robini (astigmata: acaridae).the bulb mite, rhizoglyphus robini (claparède; astigmata: acaridae), is a cosmopolitan pest with a broad host range, including commercially grown edible fungi in china. in this study, we recorded the development and reproductive traits of the bulb mite reared on four mushroom species: agaricus bisporus lange, pleurotus ostreatus kumm, pleurotus pulmonarius (fr.) quél., and lentinula edodes (berk.) pegler at six constant temperatures ranging from 15 to 31°c and 80% rh. developmental time for the ...201729202191
production of bio-fertilizer from microwave vacuum pyrolysis of palm kernel shell for cultivation of oyster mushroom (pleurotus ostreatus).microwave vacuum pyrolysis of palm kernel shell (pks) was performed to produce biochar, which was then tested as bio-fertilizer in growing oyster mushroom (pleurotus ostreatus). the pyrolysis approach produced biochar containing a highly porous structure with a high bet surface area of up to 270m2/g and low moisture content (≤10wt%), exhibiting desirable adsorption properties to be used as bio-fertilizer since it can act as a housing that provides many sites on which living microorganisms (mycel ...201729245037
water-soluble polysaccharide extracts from the oyster culinary-medicinal mushroom pleurotus ostreatus (agaricomycetes) with hmgcr inhibitory activity.water extracts from pleurotus ostreatus containing no statins showed 3-hydroxy-3-methyl-glutaryl coa reductase (hmgcr) inhibitory activity (in vitro) that might be due to specific water-soluble polysaccharides (wsps); when isolated and deproteinized, increasing concentrations of the wsp extract induced higher inhibition. the wsp extract contained mainly β-glucans, mannogalactans, and glycogen (e.g., α-glucans), although derivatives or fragments with lower molecular weights (between 14 and 3.5 kd ...201729256842
ethnomycological and nutritional analyses of some wild edible mushrooms from western himalayas, azad jammu and kashmir (pakistan).mushrooms have had a long association with humankind and have great biological and economic effects. this study aimed to record ethnomycological and nutritional analysis of some edible mushrooms from the western himalayas (neelum valley), azad jammu and kashmir, pakistan. mushrooms from different taxonomical and ecological groups were collected and properly identified with the help of the available literature: morchella esculenta, m. crassipes, m. elata, m. conica, pleurotus ostreatus, lycoperdo ...201729256848
comparison of anticancer effect of pleurotus ostreatus extract with doxorubicin hydrochloride alone and plus thermotherapy on erythroleukemia cell line.background recent studies have introduced pleurotus ostreatus (pleurotaceae) as a herbal medicine for treating different types of cancer. this survey utilizes p. ostreatus and doxorubicin hydrochloride (dox) alone and then with hyperthermia to investigate the erythroleukemia cell line. this study evaluates and compares the apoptotic and necrotic effects of various treatments on the kg-1 cell line. methods the proliferation of kg-1 cells was measured by using a tetrazolium salt (mtt)-based colori ...201729257758
increasing antioxidant activity and protein digestibility in phaseolus vulgaris and avena sativa by fermentation with the pleurotus ostreatus fungus.the aim of the research was to determine the impact of fermentation with pleurotus ostreatus on kidney beans, black beans, and oats. the results indicate that the fungus has a positive effect on the substrates when compared to the controls. the antioxidant activity (39.5% on kidney beans and 225% on oats in relation to the controls) and content of total polyphenols (kidney beans three times higher regarding the controls) increased significantly by the presence of the fungus mycelium, even after ...201729261152
diseases and pests noxious to pleurotus spp. mushroom crops.the pleurotus genus is one of most extensively studied white-rot fungi due to its exceptional ligninolytic properties. it is an edible mushroom that possesses biological effects, as it contains important bioactive molecules. it is a rich source of nutrients, particularly proteins, minerals as well as vitamins b, c and d. in basidiomycete fungi, intensive cultivations of edible mushrooms can often be affected by some bacterial, mold and virus diseases that rather frequently cause dramatic product ...201729289439
characterization of se-enriched pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities. selenium-enriched pleurotus ostreatus polysaccharide (se-pop) and the native polysaccharide (pop) were obtained by hot water extraction from fresh fruiting bodies of p. ostreatus with or without selenium. se-pop and pop had similar average molecular weights (0.95×104da and 0.94×104da, respectively). se-pop was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xy ...201729292140
different residue behaviors of four pesticides in mushroom using two different application methods.pesticide residue in mushrooms is less known. in this study, the risks of beta-cypermethrin, pyriproxyfen, avermectin, and diflubenzuron in oyster and shiitake mushrooms were evaluated using two different treatments: substrate mixture and surface spraying. almost all the concentrations of these pesticides at day 90 were higher than 80% of the initial concentrations, while it was less than 45% for all cases within 35 days by spraying. for surface spraying, the residues of beta-cypermethrin were 0 ...201829307062
enzymatic preparation of 2,5-furandicarboxylic acid (fdca)-a substitute of terephthalic acid-by the joined action of three fungal enzymes.enzymatic oxidation of 5-hydroxymethylfurfural (hmf) and its oxidized derivatives was studied using three fungal enzymes: wild-type aryl alcohol oxidase (aao) from three fungal species, wild-type peroxygenase from agrocybe aegerita (aaeupo), and recombinant galactose oxidase (gao). the effect of ph on different reaction steps was evaluated and apparent kinetic data (michaelis-menten constants, turnover numbers, specific constants) were calculated for different enzyme-substrate ratios and enzyme ...201829315223
ethanolic extract of the golden oyster mushroom, pleurotus citrinopileatus (agaricomycetes), alleviates metabolic syndrome in diet-induced obese mice.pleurotus citrinopileatus is an edible medicinal mushroom rich in biomolecules and thus has a high potential for use in formulating pharmaceutical and nutraceutical products. to test its effect on body weight and glucose control, we generated diet-induced obese (dio) c57bl/6j male mice by feeding the mice a high-fat diet (60% fat) for 8 weeks and treating them with an ethanolic p. citrinopileatus extract (pce) at either 200 or 500 mg/kg body weight for 12 additional weeks. the results showed tha ...201729345562
minimum cocktail of cellulolytic multi-enzyme complexes obtained from white rot fungi via solid-state fermentation.the objective of this work was to optimize the total cellulase activity of the crude extract cocktails from five white rot fungi produced by solid-state fermentation, by means of the central composite design. the white rot fungi pleurotus ostreatus plo 06, pleurotus eryngii ple 04, trametes versicolor tram 01, pycnosporus sanguineus pyc 02 and phanerochaete chrysosporium pc were tested. for optimization process aiming at the maximum value of total cellulase activity (fpase), the multi-enzyme cel ...201829354357
effects of pleuran (β-glucan from pleurotus ostreatus) supplementation on incidence and duration of copd exacerbations.1,3/1,6-β-glucans are recognised as immunomodulators in human and veterinary medicine for over 50 years.201729362614
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