Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| conjugal mobilization of streptococcal plasmid pmv158 between strains of lactococcus lactis subsp. lactis. | pmv158, a non-self-transmissible plasmid encoding tetracycline resistance, was conjugally transferred from enterococcus faecalis jh203 to lactococcus lactis subsp. lactis il1403. this transfer appeared to be dependent on the cotransfer of the conjugative plasmids pam beta 1 or pip501. intraspecies conjugal transfer of pmv158 also occurred in strain il1403. in contrast to the transfer from e. faecalis, transfer in il1403 did not require the presence of a conjugative plasmid in the donor strain bu ... | 1990 | 2104609 |
| synthesis and structure-activity relationships of new 7-[3-(fluoromethyl)piperazinyl]- and -(fluorohomopiperazinyl)quinolone antibacterials. | some novel 6-fluoro-7-substituted-1,4-dihydro-4-oxoquinoline-3-carboxylic acids have been prepared. at the n-1 position "standard" substitution was employed with the ethyl, cyclopropyl, and p-fluorophenyl groups being used. at c-7 the introduction of some novel piperazines was made. most notably, 2-(fluoromethyl)piperazine (10) and hexahydro-6-fluoro-1h-1,4-diazepine (16, fluorohomopiperazine) at the quinolone c-7 position produced products with similar in vitro antibacterial activity as the cip ... | 1990 | 2104934 |
| enterococcal sepsis in neonates: features by age at onset and occurrence of focal infection. | fifty-six neonates with enterococcal septicemia in a single hospital from 1977 through 1986 were studied. the incidence was low and constant until 1983, when an increase, attributable to infections in infants older than 7 days of age (late-onset), was noted. these infants were more premature (mean gestational age 29.5 vs 36.9 weeks) and had lower birth weights (mean 1250 vs 2700 g) than those with early-onset enterococcal sepsis, and in most the infections were characterized by a nosocomial orig ... | 1990 | 2104974 |
| characterization of a class of nonformylated enterococcus faecalis-derived neutrophil chemotactic peptides: the sex pheromones. | bacteria produce a heterogeneous mixture of neutrophil chemotactic agents in culture filtrates. formylmethionyl peptides have been shown to comprise a significant portion of the chemotactic activity in bacterial culture filtrates; however, not all of the chemotactic agents in bacterial culture filtrates are formylated peptides. to examine whether nonformylated peptides derived from bacteria could act as chemotactic agents, we studied several nonformylated hepta- and octapeptide enterococcus faec ... | 1990 | 2104986 |
| successful prophylaxis of experimental streptococcal endocarditis with single-dose amoxicillin administered after bacterial challenge. | rats with catheter-induced aortic vegetations were challenged intravenously with various inoculum sizes of tolerant streptococcus sanguis or streptococcus faecalis. single-dose amoxicillin (40 mg/kg) was given intravenously either 30 min before or 30-240 min after bacterial challenge. prophylaxis of endocarditis against both strains was successful when the inocula used for challenge were in the range of the minimum inoculum producing bacterial endocarditis in 90% of control animals (id90) but wa ... | 1990 | 2105361 |
| the evaluation of urine ph in screening for asymptomatic bacteriuria in pregnancy. | the purpose of this prospective investigation was to determine whether an alteration in urine ph, either by itself or in combination with other rapid screening tests, could be used to identify asymptomatic bacteriuria in pregnant women. clean catch urine specimen was used to evaluate 510 asymptomatic obstetric patients. urine specimens were tested for ph, leukocyte esterase activity, and the presence of nitrites. the ph, leukocyte esterase activity, and nitrite reaction were evaluated singly and ... | 1990 | 2106648 |
| new screening test to determine the acceptability of 0.45-micron membrane filters for analysis of water. | during routine membrane filter (mf) quality control testing, irregularities such as partial or complete inhibition of microbial growth at grid lines, abnormal spreading of colonies, growth in or along the grid lines, nonwetting areas, poor colony sheen and metallic sheen on the mf surface with mendo agar, brittleness, decreased recovery, and severe wrinkling were observed with several lots of filters. to study these effects and to develop a more sensitive screening test for mf quality, we compar ... | 1990 | 2106827 |
| interactions between antibiotics and antineoplastic drugs on antibacterial activity in vitro. | the effects of various combinations of antibacterial (ampicillin, cephadroxil, doxycycline, imipenem, trimethoprimsulfadiazine) and antineoplastic (cisplatin, epirubicin, mitoxantrone) drugs were evaluated in vitro with regard to antibacterial activity on five clinical isolates from cancer patients of respectively escherichia coli, staphylococcus aureus and streptococcus faecalis. with one exception no significant effects on the bacterial growth were observed in the presence of the antitumor dru ... | 1990 | 2106907 |
| serological response in enterococcus faecalis endocarditis determined by enzyme-linked immunosorbent assay. | enterococcus (streptococcus) faecalis expresses three species-specific surface protein antigens of molecular weights 73,000, 40,000, and 37,000. on western blotting (immunoblotting), they were detected strongly by immunoglobulin g (igg) in sera from patients with e. faecalis endocarditis, but not in sera from patients with other e. faecalis infections or with endocarditis due to other streptococci. we developed an enzyme-linked immunosorbent assay system to measure igg, igm, and iga levels to th ... | 1990 | 2107199 |
| abnormal maxillary sinus radiographs in children: do they represent bacterial infection? | thirty-three children with chronic tonsillitis and/or adenoid enlargement and without previous diagnosis of sinusitis were studied regarding the bacterial flora of their maxillary sinuses. puncture of maxillary sinus was performed at surgery (adenoidectomy and/or tonsillectomy) and aspirates were cultured. streptococcus pneumoniae was isolated from 8 of 12 (66.7%) patients whose x-rays showed completely opacified maxillary sinus. streptococcus viridans, streptococcus faecalis, and staphylococcus ... | 1990 | 2107515 |
| the enterococci: an enigma and a continuing therapeutic challenge. | 1990 | 2108029 | |
| recent taxonomic changes in the genus enterococcus. | recent taxonomic changes among the catalase-negative gram-positive cocci have led to the recognition of a separate genus for bacteria previously considered to be members of the genus streptococcus. presently, a total of 12 species of the genus enterococcus have been described. the characteristics, taxonomy and nomenclature of these species, along with the possible clinical implications of revised enterococcal taxonomy, are discussed. | 1990 | 2108030 |
| survival strategy of escherichia coli and enterococcus faecalis in illuminated fresh and marine systems. | some effects of visible light on escherichia coli and enterococcus faecalis in natural freshwater and seawater were studied by plate counts, colony area measurements, and direct counts. a large number of somnicells (non-culturable cells) were noted in illuminated systems as compared with non-illuminated ones. colony areas were significantly smaller in illuminated systems. indirect activity measurements were used to test the effects of visible light on the ability of e. coli and ent. faecalis to ... | 1990 | 2108110 |
| recurrent bacterial endocarditis in a man with tetralogy of fallot: earliest recurrence on record. | enterococcal endocarditis occurred in a young man with tetralogy of fallot who had just completed therapy for staphylococcal endocarditis. the patient had a transient reversal of flow through the ventricular septal defect during staphylococcal endocarditis with lung abscesses and had persistently elevated teichoic acid antibody titers during the second episode caused by enterococcus. our review of the literature on recurrent infective endocarditis indicates that our case represents one of the sh ... | 1990 | 2108502 |
| gentamicin-resistant enterococcal endocarditis: the need for routine screening for high-level resistance to aminoglycosides. | we have reported a case of high-level gentamicin-resistant enterococcal endocarditis as a complication of intravenous narcotic abuse. because routine screening of enterococcal blood isolates for high-level aminoglycoside resistance was not done, the patient possibly received suboptimal therapy. this case amplifies the necessity of a systematic screening program for enterococcal blood isolates to detect high-level resistance to gentamicin and streptomycin in clinical laboratories. | 1990 | 2108503 |
| enterococcal sepsis and lung microvascular injury in sheep. | in a common bile duct contamination model, we studied the effect of streptococcus faecalis compared with escherichia coli in sheep with chronic lymph fistulas to investigate the role of enterococcus in acute lung injury and acute sepsis. early pulmonary hypertension in the e coli group was not expressed in the s faecalis group, probably due to a failure of s faecalis to illicit a thromboxane a2 response. in the late period, e coli was associated with significantly greater lung microvascular dama ... | 1990 | 2108651 |
| hatching eggs sanitized with chlorine dioxide foam: egg hatchability and bactericidal properties. | the efficacy of chlorine dioxide (clo2) as an alternative sanitizing agent for hatching eggs was investigated because of the health concerns about formaldehyde fumigation. hatchability of chicken eggs was reduced when the eggs were dipped in the clo2 solutions for more than 5 minutes or in concentrations greater than 100 ppm cl. however, treating hatching eggs with a clo2 foam or fumigating with formaldehyde had no adverse effect on hatchability compared with untreated control eggs. sanitizing s ... | 1990 | 2108662 |
| species identities of enterococci isolated from clinical specimens. | conventional tests and commercially available systems were used to determine the species identities of clinical isolates of enterococci. strict adherence to the conventional test scheme of facklam and collins (r. r. facklam and m. d. collins, j. clin. microbiol. 27:731-734, 1989) resulted in the misidentification of lactose-negative enterococcus faecalis isolates as enterococcus solitarius, but this problem was overcome by the application of additional tests. the commercially available systems t ... | 1990 | 2108992 |
| recovery of vancomycin-resistant gram-positive cocci from children. | a cross-sectional survey of vancomycin-resistant gram-positive cocci (vrgpc) in the feces of children was initiated after several bacteremic infections with these organisms occurred at our hospital. a selective medium consisting of colistin-nalidixic acid agar, 5% sheep blood, vancomycin (5 mg/liter), and amphotericin b (8 mg/liter) was developed to isolate vrgpc. a single stool specimen submitted to the clinical microbiology laboratory from each of 48 patients was inoculated onto the medium. pl ... | 1990 | 2108993 |
| molecular epidemiology of beta-lactamase-producing enterococci. | plasmids from the first six reported beta-lactamase-producing (bla+) enterococci were compared for genetic relatedness. bla+ enterococcal plasmids from strains isolated in houston, tex.; philadelphia, pa.; connecticut; and pittsburgh, pa., had heterogeneous haeiii and mspi-clai restriction endonuclease digestion patterns. a staphylococcal beta-lactamase probe hybridized to all six bla+ enterococcal plasmids, but hybridization was detected on different haeiii and mspi-clai fragments of the six pl ... | 1990 | 2109577 |
| paradoxical response of enterococcus faecalis to the bactericidal activity of penicillin is associated with reduced activity of one autolysin. | ten clinical isolates of enterococcus faecalis were examined for susceptibility to the bactericidal activity of penicillin. four of these had mbcs of penicillin equal to 2 to 4 x the mic, and six exhibited a paradoxical response to penicillin, i.e., the bactericidal activity of the antibiotic had a concentration optimum at 2 to 4 x the mic and decreased significantly at concentrations above this. we found that the paradoxical response to penicillin was an intrinsic and stable property of a strai ... | 1990 | 2109578 |
| in vitro investigation of bk-218, a new oral and parenteral cephalosporin. | the antibacterial activity of bk-218 was similar to that of cefamandole when it was tested against several laboratory strains. the inhibiting effect of bk-218 was greater than that of cephalexin and cefoxitin on penicillin-binding proteins of escherichia coli hb101. this result was in close correlation with the relative inhibition of radiolabeled glucosamine incorporation (greatest with bk-218) and with the lytic effect (most intensive with bk-218). bk-218 proved to be a good inhibitor for all f ... | 1990 | 2109582 |
| recurrent postoperative endophthalmitis. | we treated five patients for postoperative endophthalmitis who demonstrated an initially good response to intravitreal management of their infection and then suffered a later recurrence. four of the five patients received a single intravitreal injection of antibiotics as the only intravitreal therapy, and the fifth patient received a single antibiotic injection in addition to a partial vitrectomy. all recurrent infections occurred between 10 and 21 days after the original intravitreal injection ... | 1990 | 2109672 |
| physical characterisation of the replication region of the streptococcus faecalis plasmid pam beta 1. | the complete nucleotide (nt) sequence of a 5.1-kb ecori dna restriction fragment carrying the replication region of the streptococcus faecalis plasmid pam beta 1 has been determined. of the seven major open reading frames (orf a-g) identified within this fragment, two (c and e) were shown to be encoding by in vitro transcription/translation assays. evidence was obtained that synthesis of the polypeptide (mr 57,380) encoded by the largest orf (e) was essential for replication. deletion analysis i ... | 1990 | 2110101 |
| a transferable plasmid associated with as-48 production in enterococcus faecalis. | enterococcus faecalis s-48 produces a peptide antibiotic, as-48, and a bacteriocin, bc-48. we have isolated mutants that lack these inhibitory characteristics. further analysis of the mutants indicates that a plasmid of 56 kilobases (pmb2) may harbor the genes for as-48. in conjugation experiments, pmb2 has been transferred into a plasmid-free og1x strain of e. faecalis. the og1x(pmb2) transconjugant produces the antibiotic as-48 in solid medium, and the mic of as-48 for this strain is the same ... | 1990 | 2110152 |
| ampicillin-resistant enterococci. | 1990 | 2110182 | |
| in vitro activity of cefpirome (hr810) against enterococcus species. | 1990 | 2110905 | |
| purulent tendon sheath infection. | 1990 | 2111007 | |
| mutation rates to 4-quinolone resistance. | the sensitivity of staph. aureus, staph. warneri, pseudomonas aeruginosa, strep. pneumoneae and strep. faecalis to eleven 4-quinolone antibacterials was compared by determining their minimum inhibitory concentrations on nutrient agar. in all cases where the minimum inhibitory concentration was less than 10 micrograms/ml then the frequency at which 4-quinolone-resistant mutants arose was estimated using each drug at 5, 10 and 20 times its minimum inhibitory concentration. it was found with all fi ... | 1990 | 2111135 |
| effects of incorporating ampicillin, bile salts and carbohydrates in media on the recognition and selection of aeromonas spp. from faeces. | the effects of incorporating ampicillin, some bile salts and sugars into media on the primary recognition and selection of aeromonads from faeces were examined. most (88%) of the 101 aeromonas strains examined had an ampicillin mic of greater than or equal to 40 mg/l, and would be isolated on blood agar containing ampicillin 30 mg/l. the strains with an ampicillin mic of less than 40 mg/l were all of human origin and predominantly a. caviae. although ampicillin at 10, 20 or 30 mg/l in blood agar ... | 1990 | 2111407 |
| [the in vitro activity of veterinary antiseptics]. | the in vitro activity of 28 veterinary antiseptic drugs was studied on 4 bacterial strains either with or without calf serum. eight preparations (vt dose, dulcidrine, collyre clément, cronic, detecaïne, lotion maudor, tano-patt, aurikan), half of which were eye-lotions, were found to be non antiseptic according to afnor standards. in the presence of calf serum, 4 other preparations (albacetine, nybocaïne, pediplasme, pedifort) did not meet the standard nf t 72.171 criteria. | 1990 | 2111651 |
| [a clinical case. infectious endocarditis]. | 1990 | 2112101 | |
| [a clinical study of asymptomatic bacteriospermia]. | asymptomatic bacteriospermia was studied as an entity and its treatment was investigated in 65 subjects who clinically needed their semen tested. the obtained results are summarized as follows. 1. the rate of detection of bacteria in semen was 89.2%, and the rates of detection of aerobic and anaerobic bacteria were 55.4% and 70.8%, respectively. forty gram-positive strains were identified among the aerobic bacteria; rates of detection for staphylococcus epidermidis and enterococcus faecalis were ... | 1990 | 2112205 |
| evidence for the translocation of enterococcus faecalis across the mouse intestinal tract. | the pathogenesis of enterococcus (streptococcus) faecalis was studied in mice with e. faecalis intestinal overgrowth (10(9) - 10(10) per gram of cecum) induced by metronidazol and streptomycin treatment coupled with oral inoculation of e. faecalis. e. faecalis was recovered from the mesenteric lymph nodes, liver, and spleen; mortality was noted in 8 (13%) of 62 mice after 14 days of e. faecalis intestinal overgrowth. light, immunofluorescent, and electron (scanning and transmission) microscopy o ... | 1990 | 2113080 |
| biological characterization of a new radioactive labeling reagent for bacterial penicillin-binding proteins. | radiolabeled penicillin g is widely used as the imaging agent in penicillin-binding protein (pbp) assays. the disadvantages of most forms of labeled penicillin g are instability on storage and the long exposure times usually required for autoradiography or fluorography of electrophoretic gels. we investigated the utility of radioiodinated penicillin v as an alternative reagent. radioiodination of p-(trimethylstannyl)penicillin v with [125i]na, using a modification of the chloramine-t method, is ... | 1990 | 2113792 |
| amino acid sequence of pheromone-inducible surface protein in enterococcus faecalis, that is encoded on the conjugative plasmid ppd1. | the major pheromone-inducible protein, pd78, believed to contribute to bacterial conjugation, was purified from enterococcus (formerly streptococcus) faecalis cells containing the plasmid ppd1. a cloned ecori-bglii 3.6-kbp fragment of the plasmid pam351 (ppd1::tn916) contained an open reading frame corresponding to 467 amino acid residues representing pd78. in a central region of the deduced protein, there is a repeated sequence of x-x-pro that is repeated 15 times. this is analogous to the gln- ... | 1990 | 2114324 |
| growth rate, proteolysis and acid production of streptococcus faecalis subsp. liquefaciens in skim milk with some additives. | growth rate, proteolysis and acid production of streptococcus faecalis subsp. liquefaciens were examined in skim milk with and without 0.15% calcium lactate, 0.03 m na, k phosphate, 5% caco3 or 2-8% nacl, at 30 degrees c. calcium lactate affected neither the growth rate nor the breakdown of casein. the highest acid production and degradation of casein occurred in phosphate-buffered skim milk (ph 7.1). inorganic phosphate did not have any influence on the generation time and hydrolysis of casein. ... | 1990 | 2114547 |
| [studies on delayed skin reactivity to streptococci in behçet's disease]. | delayed type hypersensitivity of patients with behçet's disease was investigated by intradermal skin test and prick test, using various inactivated microorganisms as antigens. in the intradermal skin test, 13 patients showed significantly increased skin reactivity to e. faecalis, compared with 5 normal controls. no significant differences were observed between these two groups in terms of reaction to s. pyogenes. prick test was performed in 11 patients and 6 controls, using s. pyogenes, s. sangu ... | 1990 | 2114734 |
| effect of in vivo growth conditions upon expression of surface protein antigens in enterococcus faecalis. | western blotting of whole-cell preparations of enterococcus faecalis showed the protein-antigen profiles to be markedly influenced by growth conditions. the e. faecalis-specific antigens of 40 and 37 kda, which are prominent in endocarditis, were strongly expressed following growth in serum or brain heart infusion, but not after growth in a chemically defined medium. the expression of these antigens in vivo was demonstrated in cells grown as a biofilm on silastic discs in the peritoneum of rabbi ... | 1990 | 2114887 |
| a case of bacteremia and a hepatic lesion. | 1990 | 2115611 | |
| [evaluation of microbial indicators of health risk related to river swimming places]. | a retrospective follow-up study was conducted among 5,737 tourists in 8 holiday camps, during the 1986 summer, in the ardèche river basin, in order to study the relationship between the bacteriological water quality and the morbidity that occurred to swimmers. total morbidity was more frequent among bathers than non-bathers (risk ratio = 1.7 [95% confidence interval = 1.4-2.1]); gastro-intestinal illness was the major type of morbidity (rr = 2.8 [1.8-4.4] for "objective gastro-intestinal disease ... | 1990 | 2115683 |
| the structure of nadh peroxidase from streptococcus faecalis at 3.3 a resolution. | nadh peroxidase (ec 1.11.1.1) previously isolated from streptococcus faecalis 10c1 has been crystallized. the crystal structure has been solved by multiple isomorphous replacement and solvent-flattening at 3.3 a (1 a = 0.1 nm) resolution. the enzyme forms a tetramer consisting of 4 crystallographically related subunits. the monomer chain fold is in general similar to those of glutathione reductase and lipoamide dehydrogenase. fad binds in the same region and in a similar conformation as in gluta ... | 1990 | 2116319 |
| genetic stability of the antagonistic character of enterococcus faecalis ssp. liquefaciens and the detection of a new inhibitory bacteriocin-like substance. | the inhibitory capacity of strain s-48 of enterococcus faecalis ssp. liquefaciens was studied. the strain produces a broad-spectrum peptide antibiotic (as-48) that has been characterized elsewhere. the isolation of mutants from s-48 after mutagenic treatment revealed another inhibitory substance which remained masked in the wild strain. the protein nature and restricted spectrum of this substance points to its being a bacteriocin. | 1990 | 2116372 |
| induction of inhibitory agent produced by enterococcus faecalis. | the effect of treatment with inducing agents, such as mitomycin c, hydrogen peroxide and uv irradiation on the production of two inhibitors by different mutants from enterococcus faecalis s-48 was studied. with hydrogen peroxide and uv light no increase in either the absolute or the relative amount of antagonistic substances was observed. with mitomycin c, a significant increase in the individual cell capacity for inhibitor production was detected. | 1990 | 2116373 |
| evaluation of microscan for identification of enterococcus species. | emerging drug resistance of the enterococci necessitates differentiation from group d streptococci and accurate species identification. microscan (baxter healthcare corp., west sacramento, calif.) has recently developed a microdilution system for identification and antibiotic susceptibility testing of gram-positive cocci. to evaluate the ability of this system to identify enterococcus species, 100 isolate identified as enterococci by microscan were tested by conventional media and 60 isolates of ... | 1990 | 2116449 |
| amino acid sequence of pheromone-inducible surface protein in enterococcus faecalis, that is encoded on the conjugative plasmid ppd1. | the major pheromone-inducible protein, pd78, believed to contribute to bacterial conjugation, was purified from enterococcus (formerly streptococcus) faecalis cells containing the plasmid ppd1. a cloned ecori-bglii 3.6-kbp fragment of the plasmid pam351(ppdl::tn916) contained an open reading frame corresponding to 467 amino acid residues representing pd78. in a central region of the deduced protein, there is a repeated sequence of x-x-pro that is repeated 15 times. this is analogous to the gln-g ... | 1990 | 2116976 |
| high-level gentamicin resistance in enterococcus: microbiology, genetic basis, and epidemiology. | antibiotic resistance is an ever-increasing problem in enterococci. these bacteria are remarkable in their ability to acquire and disseminate antibiotic resistance genes by a variety of routes. since first described in 1979, high-level resistance to gentamicin (mic, greater than 2,000 micrograms/ml) has spread worldwide and has been responsible for serious infections. resistance is plasmid-mediated and due to aminoglycoside-modifying enzymes. high-level gentamicin resistance indicates that there ... | 1990 | 2117300 |
| win 57273, a new fluoroquinolone with enhanced in vitro activity versus gram-positive pathogens. | win 57273 is a new fluoroquinolone with excellent in vitro activity versus gram-positive pathogens, including methicillin-susceptible and -resistant staphylococcus aureus and staphylococcus epidermidis and gentamicin-susceptible and -resistant enterococcus faecalis. we compared the microdilution mics and mbcs of this compound to those of other antimicrobial agents for more than 30 clinical isolates of each of these groups of organisms and found that with few exceptions, it was at least 10 times ... | 1990 | 2117417 |
| does a tetracycline resistance determinant of class n exist? | pmv120 was reported to carry the tetracycline resistance (tcr) determinant of class n. we obtained tetracycline-susceptible transconjugants harboring plasmids with restriction enzyme profiles indistinguishable from those of pmv120 isolated from tetracycline-resistant clones. we conclude that pmv120 is a cryptic plasmid and that class n of tcr determinants does not exist. | 1990 | 2117419 |
| in vitro susceptibilities of oxacillin-resistant staphylococcus aureus to dapsone and sulfamethoxazole alone and in combination with trimethoprim. | the in vitro activity of trimethoprim plus dapsone against oxacillin-resistant staphylococcus aureus was comparable to that of trimethoprim plus sulfamethoxazole. because of its different pharmacologic properties, dapsone may be a useful agent in combination with trimethoprim for the treatment of patients colonized with oxacillin-resistant s. aureus. | 1990 | 2117420 |
| isolation of three strains of beta-lactamase-producing enterococcus faecalis in argentina. | of the 90 strains of enterococcus spp. isolated in an argentine laboratory in the period from january to july 1989, three were identified as beta-lactamase-producing enterococcus faecalis. according to the literature reviewed, these appear to be the first beta-lactamase-positive strains of enterococcus isolated outside the usa. they differed from most beta-lactamase-producing strains already described in that they were susceptible to low concentrations of macrolides. all three strains were resis ... | 1990 | 2117531 |
| infection of the symphysis pubis by streptococcus faecalis. | 1990 | 2117662 | |
| genetic functions and cell-cell interactions in the pheromone-inducible plasmid transfer system of enterococcus faecalis. | pheromone-inducible plasmid transfer is a novel form of bacterial conjugation which has, to date, been observed only in enterococcus (streptococcus) faecalis. this process includes several important stages of interaction between the donor and recipient cell. the initial interaction is the transmission of a chemical signal from the recipient to the donor cell. recent evidence has shown that the signal is in the form of a small hydrophobic peptide, which is capable of inducing a complex mating res ... | 1990 | 2117692 |
| antibacterials. synthesis and structure-activity studies of 3-aryl-2-oxooxazolidines. 2. the "a" group. | the synthesis and structure-activity relationship (sar) studies of the effect of varying the "a" group in a series of 5-(acetamidomethyl)oxazolidonone antibacterials (2) are described. compounds 2 were principally prepared either by the six-step synthesis described previously (j. med. chem. 1989, 32, 1673) or by elaboration of the synthetic intermediate 2 (a = h) via electrophilic aromatic substitution or elaboration of the intermediate 2 (a = i) by transition metal catalyzed carbon-carbon bond- ... | 1990 | 2118186 |
| chemoprophylactic efficacy against experimental endocarditis caused by beta-lactamase-producing, aminoglycoside-resistant enterococci is associated with prolonged serum inhibitory activity. | we studied the prevention of experimental aortic endocarditis caused by a beta-lactamase-producing, aminoglycoside-resistant strain of enterococcus faecalis (hh22) in 146 catheterized rabbits. both vancomycin and ampicillin-sulbactam readily killed this resistant enterococcus strain in vitro. at a challenge inoculum of approximately 10(9) cfu, vancomycin (40 mg/kg intravenously [i.v.]), ampicillin (40 mg/kg i.v.), or a combination of ampicillin plus a beta-lactamase inhibitor, sulbactam (20 mg/k ... | 1990 | 2118323 |
| antibacterial activity of lomefloxacin in a pharmacokinetic in vitro model. | an in vitro model simulating two-compartment pharmacokinetics was used to study the antibacterial activity of lomefloxacin after single oral doses of 200 and 400 mg. lomefloxacin produced reliable bactericidal activity against gram-negative aerobic bacteria and staphylococci. bacterial strains for which mics were less than 0.5 microgram/ml were inhibited with both dosing schedules. doubling the dose from 200 to 400 mg increased the bactericidal activity only against pseudomonas aeruginosa. again ... | 1990 | 2118324 |
| evidence for a staphylococcal-like mercury resistance gene in enterococcus faecalis. | we investigated mercury resistance (hgr) in 52 clinical isolates of enterococcus faecalis from two different geographical regions. eleven of the 52 strains were resistant to hgcl2, and plasmids from these enterococci hybridized with a staphylococcal mercury resistance gene probe. hgr from 5 of the 11 transferred at frequencies ranging from approximately 2 x 10(-7) to 2 x 10(-3). | 1990 | 2118329 |
| involvement of pyruvate dehydrogenase in product formation in pyruvate-limited anaerobic chemostat cultures of enterococcus faecalis nctc 775. | enterococcus faecalis nctc 775 was grown anaerobically in chemostat culture with pyruvate as the energy source. at low culture ph values, high in vivo and in vitro activities were found for both pyruvate dehydrogenase and lactate dehydrogenase. at high culture ph values the carbon flux was shifted towards pyruvate formate lyase. some mechanisms possibly involved in this metabolic switch are discussed. in particular attention is paid to the nadh/nad ratio (redox potential) and the fructose-1,6-bi ... | 1990 | 2118752 |
| comparison of vancomycin-inducible proteins from four strains of enterococci. | vancomycin-inducible proteins of 39.5 and 39 kda from respectively, low-level and high-level resistant enterococci were compared. electrophoretic, immunoblot and peptide analysis revealed three types of protein, one in a low-level resistant strain of e. faecium, one in 2 high-level-resistant strains of e. faecium, and one in a high-level resistant strain of e. faecalis. the inducible proteins of e. faecium and e. faecalis, of 39.5 and 39 kda respectively, which may function in a similar fashion ... | 1990 | 2118867 |
| validation of flow cytometry for rapid enumeration of bacterial concentrations in pure cultures. | flow cytometry was investigated as a rapid detection and counting method for bacteria in pure cultures. a simple two-parameter detection scheme was employed: particle size was measured by forward angle light scatter and nucleic acid content by fluorescence of the dna/rna-binding dye ethidium bromide. the technique gave results that correlated exceptionally well with conventional plate counting for four species of bacteria, and concentrations in the range 10(2) to 10(7) cfu/ml. cytometric counts ... | 1990 | 2118900 |
| studies on the synthesis, characterisation and antimicrobial activity of new co(ii), ni(ii) and zn(ii) complexes of schiff base derived from ninhydrin and glycine. | for the first time, co(ii), ni(ii) and zn(ii) complexes have been synthesized involving an intermediate schiff base, indane-1,3-dione-2-imine-n-acetic acid the condensed product of ninhydrin and glycine. these coloured complexes were characterised by elemental analysis, molar conductivity, thermogravimetric analyses/differential thermal analysis, infrared, magnetic susceptibility, nmr and electronic spectral studies. mechanisms for their formation have been proposed. the experimental studies rev ... | 1990 | 2119197 |
| [the conjugative transfer of beta-hemolysin plasmid in enterococcus faecalis to enterococcus faecium]. | the conjugative transfer by using a filter mating method in 109 cases of combinations between 20 strains of beta-hemolytic enterococcus faecalis and 28 strains of alpha-hemolytic enterococcus faecium was tried. a stable transconjugation was observed in two cases: from e. faecalis no. 170 to e. faecium no. 49 and from e. faecalis no. 500 to e. faecium no. 48. from the results, it was proved that the beta-hemolysin plasmid of e. faecalis is transferred by conjugation to e. faecium. the virulence ( ... | 1990 | 2119404 |
| [the virulence of enterococcus faecalis in experimental urinary tract infection in mice]. | the virulence of enterococcus faecalis in the urinary tract in mice was studied. pyelonephritis was distinctly caused by e. faecalis which have a high affinity to mouse kidneys. this infection was continuous, but did not turn into sepsis. the relationship between the production of hemolysin or protease and the virulence in the urinary tract was investigated without finding any distinct association. it is concluded that e. faecalis is a pathogenic organism in the urinary tract and presumably indu ... | 1990 | 2119407 |
| antibiotic resistance in enterococci in nottingham. | 1990 | 2120172 | |
| treatment of postpartum endometritis. | in this study, cefoxitin alone (2 gm, every 8 hours) was generally sufficient to cure postpartum endometritis, even when the patient had received prophylaxis with a beta-lactam antibiotic. when single-agent therapy fails, addition of ampicillin usually results in a prompt clinical response. | 1990 | 2120267 |
| the growth-inhibitory effect of the enterococcus faecalis bacteriocin encoded by pad1 extends to the oral streptococci. | many strains of enterococcus faecalis, a normal inhabitant of the oral cavity, elaborate a plasmid-encoded bacteriocin. the growth-inhibitory effect of this bacteriocin was observed to extend to a variety of pathogenic oral streptococci, including those that play a major role in tooth surface colonization and caries formation. these results suggest that transient colonization by bacteriocin-producing e. faecalis may effect shifts in oral colonization by susceptible organisms and that bacteriocin ... | 1990 | 2120304 |
| [fever during labor. a prospective study of 6,305 deliveries]. | 1990 | 2120318 | |
| the bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. a microbiological and spectroscopic study. | the combined antibacterial activity of various porphyrins with hemin on gram-positive bacteria was studied. protoporphyrin, hematoporphyrin derivative and deuteroporphyrin show only a marginal inhibitory effect in the dark. however, hemin has a strong cytotoxic effect which is independent of illumination and is equally strong in the dark. the disadvantage of hemin treatment is that it is temporary. in this study, we have demonstrated that a combination of deuteroporphyrin and hemin has a unique ... | 1990 | 2120409 |
| sequence analysis of enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pad1. | the location of the structural gene for aggregation substance on the sex pheromone plasmid pad1 of enterococcus faecalis was determined using an oligonucleotide deduced from the n-terminal amino acid sequence of the purified protein. the nucleotide sequence was determined for the corresponding region and two open reading frames (orfs) could be identified. orf1 codes for a small (mr 13,160) acidic protein of unknown function. the gene for aggregation substance (named asa1) was found to code for a ... | 1990 | 2120541 |
| maintenance of a neutral cytoplasmic ph is not obligatory for growth of escherichia coli and streptococcus faecalis at an alkaline ph. | both escherichia coli and streptococcus faecalis grew in an alkaline medium containing 100 microm carbonyl cyanide m-chlorophenylhydrazone (cccp). our data suggested that cccp functioned as a protonophore at a high ph and that the proton-motive force was dissipated almost completely under such conditions. the ph gradient measured with dimethyloxazolidine-2,4-dione and acetylsalicylic acid was very small in both bacteria at a high ph above 8, and was not affected significantly by the addition of ... | 1990 | 2121723 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1987). i. susceptibility distribution]. | since 1979, ikemoto et al. have been retrospectively surveying the sensitivity of major species of bacteria isolated from patients with urinary tract infections to various antibacterial agents and antibiotics. their findings for the past year are reported below. a total of 825 clinical strains of bacteria was investigated. of this total, gram-positive bacteria accounted for 28.0% (231 strains) and gram-negative bacteria for 72.0% (594 strains). taxonomically, escherichia coli accounted for 34.7% ... | 1990 | 2122040 |
| recurrent episodes of enterococcaemia from an infected hickman line precipitated by ganciclovir infusion. | 1990 | 2122430 | |
| epidemiology of high-level gentamicin resistant enterococcal isolates from zimbabwe. | high-level gentamicin resistance (minimum inhibitory concentration of greater than or equal to 2,000 mcg/ml) in enterococcus faecalis has not previously been reported in africa to our knowledge. eight of 28 (29%) rectal swab specimens obtained from hospitalized patients in zimbabwe had gentamicin resistant enterococci. previous exposure to penicillins or aminoglycosides were risk factors for colonization with these organisms. this study documents the presence of high-level gentamicin resistant e ... | 1990 | 2122746 |
| quantitative changes in faecal microflora preceding necrotising enterocolitis in premature neonates. | quantitative studies of faecal bacterial flora were carried out during the week preceding the clinical onset of 12 episodes of neonatal necrotising enterocolitis. there were considerable quantitative changes in the faecal flora preceding the clinical onset of both definite and possible episodes of necrotising enterocolitis. there was a decline in the numbers of some species from up to 72 hours before the clinical onset of the disease. enterobacteriaceae were isolated from samples collected durin ... | 1990 | 2122814 |
| rapid dissemination of beta-lactamase-producing, aminoglycoside-resistant enterococcus faecalis among patients and staff on an infant-toddler surgical ward. | 1990 | 2123301 | |
| an hlyb-type function is required for expression of the enterococcus faecalis hemolysin/bacteriocin. | the nucleotide sequence of a 3,422-bp internal restriction fragment from the enterococcus faecalis pad1 hemolysin/bacteriocin-encoding region was determined. this fragment was associated with expression of hemolysin/bacteriocin component l and contained a 2,142-bp open reading frame. the inferred amino acid sequence revealed a protein which shared extensive similarity with hlyb of the escherichia coli alpha-hemolysin operon. the inferred protein, cylb, was observed to be independently expressed ... | 1990 | 2123826 |
| physiological studies on regulation of glycerol utilization by the phosphoenolpyruvate:sugar phosphotransferase system in enterococcus faecalis. | in vitro studies with purified glycerol kinase from enterococcus faecalis have established that this enzyme is activated by phosphorylation of a histidyl residue in the protein, catalyzed by the phosphoenolpyruvate-dependent phosphotransferase system (pts), but the physiological significance of this observation is not known. in the present study, the regulation of glycerol uptake was examined in a wild-type strain of e. faecalis as well as in tight and leaky ptsi mutants, altered with respect to ... | 1990 | 2123855 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| [experimental study of the course of the immune response at the urinary level following repeated oral vaccination]. | the progression of the specific immunoreactions in the urines of rabbits vaccinated by oral route from 1 to 10 times with 20-40 billion of some antigens (e. coli, proteus vulgaris, staph aureus, str. faecalis), administered daily or by-weekly, has been evaluated by agglutination and elisa. the immunoenzymatic elisa technique has revealed increases of specific immunoglobulins also one day after the first oral administration of the antigens and a final progression of iga titres (after ten treatmen ... | 1990 | 2123973 |
| [fever, somnolence, vomiting, diarrhea]. | a 69-year-old female was admitted because of a febrile state, somnolence, nausea and diarrhea. addison's disease known to have developed several years earlier after adrenal tuberculosis suggested addisonian crisis triggered by an acute infection. therapy with steroids and substitution of electrolytes and fluid led to rapid improvement. streptococcus bovis was identified as causative agent. the known association of this germ with cancer of the colon led to further investigation which revealed can ... | 1990 | 2124719 |
| inhibitory activity of papain on facultative pathogens--short communication. | papain, a proteolytic enzyme obtained from papaya was found inhibitory on few facultative pathogens. while escherichia coli, staphylococcus albus, klebsiella pneumoniae and enterobacter cloacae were inhibited at concentration from 200 ppm on the cell density of staphylococcus faecalis decreased only at higher concentration of papain (400-800 ppm). | 1990 | 2124762 |
| mutants of enterococcus faecalis deficient as recipients in mating with donors carrying pheromone-inducible plasmids. | from enterococcus faecalis cells containing random chromosomal insertions of tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. the phage-resistant strains all showed decreased recipient ability (con-) in broth matings with donors carrying pheromone-inducible plasmids. these strains were normal with respect to donor ability in broth matings and recipient ability in filter matings. the data suggest that the mutants are deficient in the binding substanc ... | 1990 | 2125350 |
| an investigation of indirect conductimetry for detection of some food-borne bacteria. | indirect conductimetry using a rapid automated bacterial impedance technique was investigated. strains of staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, aeromonas hydrophila and salmonella spp. grown in whitley impedance broth all elicited indirect conductimetric changes. these indirect conductance responses were improved by the addition of 2 g/l glucose to the medium and resulted in maximum changes of 2340-4300 ... | 1990 | 2126006 |
| high efficiency introduction of plasmid dna into glycine treated enterococcus faecalis by electroporation. | a highly efficient electroporation system for enterococcus faecalis was developed by systematically optimizing different parameters. one parameter found to be particularly critical for electroporation was cultivation of e. faecalis in medium containing a high glycine concentration, prior to electroporation. osmotic stabilization of cells with 0.5 m sucrose was also found to be critical during glycine treatment. 10(6) transformants per microgram of plasmid dna were consistently obtained within 48 ... | 1990 | 2126058 |
| histamine formation by enterococci in goat cheese. | cheeses were made of heat-treated goat milk inoculated with large numbers of a histamine-producing strain of streptococcus faecium or a non-histamine-producing strain of s. faecalis. every second week during ripening (91 days) the cheeses were sampled for histamine analyses and bacteriological analyses. the numbers of enterococci remained high throughout the whole period of investigation and the maximum amount of histamine detected was 8.2 micrograms/g in one of the cheeses. the enterococci seem ... | 1990 | 2126443 |
| conjugal transfer of plasmid pwbg637 from staphylococcus aureus to staphylococcus epidermidis and streptococcus faecalis. | plasmids pwbg636 (gmr) and pwbg642 (emr) derived from the conjugative plasmid pwbg637 were tested for ability to transfer conjugatively to staphylococcus epidermidis, streptococcus faecalis, bacillus subtilis and escherichia coli. both plasmids transferred to s. epidermidis and strept. faecalis but not to b. subtilis and e. coli. once in s. epidermis and strept. faecalis they were transferred back to s. aureus. restriction endonuclease analysis showed that the plasmids were stably maintained in ... | 1990 | 2126516 |
| [clinical analysis of streptococcus faecalis septicemia in children]. | the clinical analysis of 59 cases of streptococcus faecalis sepsis in infants aged 1 to 5 months has been presented. streptococcus faecalis strains isolated from the blood were characterized by drug resistance especially against penicillin or its derivatives. the clinical picture of the streptococcus faecalis sepsis in a majority of cases is similar to that of other gram-positive sepsis. meningitis, first of all purulent, was noted in twenty percent of infected children. about one third of neona ... | 1990 | 2126632 |
| nonenterococcal group d streptococcal septicemia: association with unrecognized endocarditis. | 68 patients presented to the veterans general hospital, taipei with nonenterococcal group d streptococcal septicemia in the years 1985-1987. 36 patients (53%) had nonenterococci as part of a polymicrobial bacteremia. the large intestine was not examined in most patients. five patients (7%) had associated colonic carcinoma, and 17 patients (25%) had colorectal diseases. only 7/68 patients (10%) were clinically diagnosed as having infective endocarditis by the doctors in charge. the others were re ... | 1990 | 2126642 |
| in vitro antibacterial activity of faeriefungin, a new broad-spectrum polyene macrolide antibiotic. | the in vitro activity of faeriefungin, a new pentaene macrolide lactone antibiotic produced by streptomyces griseus var. autotrophicus, against 263 clinical bacterial isolates was examined. in contrast to the related fungicidal antibiotics nystatin and amphotericin b, which show no activity against bacteria, faeriefungin showed bactericidal activity against all species of gram-positive bacteria examined. mics for these species ranged from 8 to 64 micrograms/ml, and the mic for 90% of the isolate ... | 1990 | 2126689 |
| failure of trimethoprim-sulfamethoxazole therapy in experimental enterococcal endocarditis. | to assess the potential efficacy of trimethoprim-sulfamethoxazole (tmp-smx) against serious enterococcal infections, we used a rat enterococcal endocarditis model comparing tmp-smx therapy (500 mg of tmp plus 2,500 mg of smx per kg of body weight per day given every 8 h by intragastric gavage) with intravenous ampicillin therapy (1,000 mg/kg per day). despite concentrations of active drug in serum well in excess of the mic and mbc, the mean residual vegetation bacterial titer in tmp-smx-treated ... | 1990 | 2126691 |
| efficacy of ampicillin versus trimethoprim-sulfamethoxazole in a mouse model of lethal enterococcal peritonitis. | lethal enterococcal peritonitis in mice was used to compare trimethoprim-sulfamethoxazole (tmp-smx) therapy with ampicillin therapy. peritoneal fluid showed a 10(3)-cfu decrease in enterococci with ampicillin compared with tmp-smx. mortality of the untreated mice was 100%, compared with 40% for ampicillin and 95% for tmp-smx, despite adequately measured levels in serum and peritoneal fluid. | 1990 | 2126692 |
| in vitro activity of dirithromycin (ly 237216) compared with activities of other macrolide antibiotics. | dirithromycin inhibited streptococcus pyogenes, streptococcus pneumoniae, and other hemolytic streptococci at concentrations of less than or equal to 0.03 to 0.12 micrograms/ml, with 90% inhibition at 0.12 micrograms/ml, which is comparable to results using erythromycin. group a streptococci, listeriae, and enterococci resistant to erythromycin were resistant to dirithromycin. erythromycin-susceptible staphylococci were inhibited by 0.5 micrograms/ml, but for erythromycin-resistant isolates mics ... | 1990 | 2126695 |
| in vitro detection of enterococcal vancomycin resistance. | by using agar dilution as the standard method, we determined the ability of broth microdilution, disk diffusion, and an automated system (ams vitek) to detect three different levels of vancomycin resistance among six enterococcal isolates. enterococcus gallinarum aib-38 and aib-39 exhibited low-level resistance (mic, 16 to 32 micrograms/ml) that was detected only by agar and broth dilution methods. e. faecalis v583, e. faecium aib-42, and e. faecalis aib-41 showed moderately high-level resistanc ... | 1990 | 2126696 |
| in vitro activity of bay v 3522, a new oral cephalosporin. | the in vitro activity of bay v 3522 was compared with the activities of cephalexin, cefaclor, cefuroxime, cefixime, and amoxicillin-clavulanate. mics (in micrograms/ml) of bay v 3522 were as follows: staphylococcus spp. (except for oxacillin-resistant strains), 0.13 to 1; streptococcus spp. (except for some viridans group streptococci), less than or equal to 0.015 to 0.25; enterococcus faecalis, 2 to 8; other enterococci, 0.5 to greater than 32; beta-lactamase-negative haemophilus influenzae and ... | 1990 | 2126697 |
| [experimental criteria for interpretation of bacterial sensitivity to dioxidine determined by diffusion from the disks]. | antibacterial activity of dioxidine against aerobic and facultative anaerobic organisms under conditions of anaerobiosis i. e. conditions really observed for example in abscess cavities or necrotic tissues is 30 to 100 times as high as that under aerobic conditions. there is a relationship between sensitivity of bacteria to dioxidine under aerobic and anaerobic conditions which is expressed by the regression equation. therefore, comparison of the mics determined under anaerobic conditions with t ... | 1990 | 2126705 |
| [optimal control of the microflora environment in suppurative foci]. | the informative capacity of various statistic parameters characterizing the ecology of pathogens of purulent processes was studied. many-years' results of the routine bacteriological tests of the materials from purulent wounds in patients from surgical, traumatological and burn departments served as the study subjects. the frequency, persistence and contamination capacity of the pathogens were investigated. data on the changes in the frequency parameter with respect to the main pathogens of woun ... | 1990 | 2126707 |
| enterococcus faecalis and otitis media with effusion. how to treat. | although microorganisms are cultured in only one out of 3 middle ear effusions, viable and non-viable bacteria are presumed to be responsible in part for otitis media with effusion (ome). because of this association, antibiotics in sublethal, bacteriostatic, or bacteriocidal concentrations are frequently used as non-surgical therapy for ome. antibiotic treatment is predicated on the assumption that microorganisms responsible for ome are the same ones which produce acute otitis media. this may no ... | 1990 | 2126780 |
| growth inhibition of selected food-borne bacteria, particularly listeria monocytogenes, by plant extracts. | six extracts from chinese medicinal plants: tin men chu, sey lau pai, siu mao heung, bak tao yung, kam chin chiu and liao ya, were tested for their inhibitory effect on selected food-borne bacteria by the well assay technique. among them, tin men chu, siu mao heung and sey lau pai inhibited the growth of staphylococcus aureus, klebsiella pneumonia, escherichia coli, shigella flexneri, streptococcus faecalis, salmonella paratyphi, salm. enteritidis, enterobacter aerogenes, pseudomonas fluorescens ... | 1990 | 2127264 |
| a model for aggregation-dispersion dynamics of a population. | an important feature that distinguishes the movement of living systems from the random motion of inorganic material is a delicate balance between spreading and concentrating. this movement is based on the kind of interactions which a bacterial colony may establish during migration. namely, the antagonistic effects of dispersal which take place preferentially down the population gradient and the tendency in grouping together. in this work a model is proposed which considers these effects. the pha ... | 1990 | 2127375 |
| lactic acid production by membrane reactors. | 1990 | 2127523 |