Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| sialic acid-dependent interactions between influenza viruses and streptococcus suis affect the infection of porcine tracheal cells. | bacterial co-infections are a major complication in influenza-virus-induced disease in both humans and animals. either of the pathogens may induce a host response that affects the infection by the other pathogen. a unique feature in the co-infection by swine influenza viruses (siv) and streptococcus suis serotype 2 is the direct interaction between the two pathogens. it is mediated by the haemagglutinin of siv that recognizes the α2,6-linked sialic acid present in the capsular polysaccharide of ... | 2015 | 26297001 |
| catabolite control protein a has an important role in the metabolic regulation of streptococcus suis type 2 according to itraq-based quantitative proteomic analysis. | the catabolite control protein a (ccpa) regulates the carbon metabolism in streptococcus suis type 2 and has pleiotropic regulatory functions in bacterial virulence and transcription. the present study systematically investigated ccpa activity in streptococcus suis type 2 using isobaric tag for relative and absolute quantification (itraq) liquid chromatography‑tandem mass spectrometry‑based proteomics. gene ontology and kyoto encyclopedia of genes and genomes analyses demonstrated that ccpa is a ... | 2015 | 26299628 |
| virulence genotyping and population analysis of streptococcus suis serotype 2 isolates from china. | streptococcus suis is one of the most important swine pathogens worldwide. in this study, a total of 22 virulence-related genes in 101 strains of s. suis serotype 2 (ss2) were detected by pcr, namely, mrp, epf, sly, fbps, rgg, ofs, srta, pgda, gapdh, iga, endod, ciarh, salkr, mann, purd, rgg, dppiv, neub, dlta, smu_61-like, spym3_0908 (permease) and the sspa gene. the distribution of virulence-related genes among isolates was visualized using bionumerics software to study similarities among the ... | 2015 | 26303637 |
| antioxidant activity and antibacterial effects on clinical isolated streptococcus suis and staphylococcus intermedius of extracts from several parts of cladogynos orientalis and their phytochemical screenings. | the in vitro antioxidant and antibacterial assays against clinically isolated streptococcus suis and staphylococcus intermedius of the extracts prepared by decoction and ethanolic reflux of different parts of chettaphangki (cladogynos orientalis zipp. ex span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (dpph) scavenging assay and disc diffusion method were conducted. quantitative analysis of total phenolic and total flavonoid contents in the extracts using spect ... | 2015 | 26347795 |
| assessment of maldi-tof ms as alternative tool for streptococcus suis identification. | the accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for identifying streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a pcr-based identification assay as the gold standard. in addition, maldi-tof ms was compared with the commercial multi-tests rapid id 32 strep system. from the 129 s. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score val ... | 2015 | 26347858 |
| emergence of streptococcus suis serotype 9 infection in humans. | 2015 | 26362754 | |
| expression, purification, crystallization and structure determination of the n terminal domain of fhb, a factor h binding protein from streptococcus suis. | fhb is a surface virulence protein from streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor h to inactivate c3b deposited on bacterial surface in blood. here we successfully expressed and purified the n terminal domain of fhb (n-fhb) and obtained crystals of the n-fhb by sitting-drop vapor diffusion method with a resolution of 1.50 å. the crystals belong to space group c2 with unit cell parameters a = 127.1 å, b = 77.3 å, ... | 2015 | 26365348 |
| assessing the metabolic diversity of streptococcus from a protein domain point of view. | understanding the diversity and robustness of the metabolism of bacteria is fundamental for understanding how bacteria evolve and adapt to different environments. in this study, we characterised 121 streptococcus strains and studied metabolic diversity from a protein domain perspective. metabolic pathways were described in terms of the promiscuity of domains participating in metabolic pathways that were inferred to be functional. promiscuity was defined by adapting existing measures based on dom ... | 2015 | 26366735 |
| complex population structure and virulence differences among serotype 2 streptococcus suis strains belonging to sequence type 28. | streptococcus suis is a major swine pathogen and a zoonotic agent. serotype 2 strains are the most frequently associated with disease. however, not all serotype 2 lineages are considered virulent. indeed, sequence type (st) 28 serotype 2 s. suis strains have been described as a homogeneous group of low virulence. however, st28 strains are often isolated from diseased swine in some countries, and at least four human st28 cases have been reported. here, we used whole-genome sequencing and animal i ... | 2015 | 26375680 |
| investigations of selected pathogens among village pigs in central papua, indonesia. | village pig husbandry is an important part of livestock production in papua province, eastern indonesia. however, high level of disease and mortality constrains production. the aim of this study was to investigate the prevalence of the selected pathogens in village pigs in the jayawijaya region of papua province, indonesia. two studies were conducted: study 1 determined the prevalence of selected pathogens in dead or moribund pigs sent to the main local market for sale. study 2 recorded the prev ... | 2016 | 26381546 |
| multi-etiological nature of tuberculosis-like lesions in condemned pigs at the slaughterhouse. | tuberculosis-like lesions (tbl) in pigs have been associated with microorganisms other than mycobacteria. in this work a histopathological and microbiological evaluation of tbl in pigs is shown. a total of 352 samples belonging to 171 pigs totally condemned at slaughterhouse due to generalized tbl were sampled and selected for analysis. pyogranulomatous (56.2%) and granulomatous lesions (20.2%) were observed in all analysed organs. most of the granulomas observed in both lymph nodes and lungs be ... | 2015 | 26418681 |
| first human case report of sepsis due to infection with streptococcus suis serotype 31 in thailand. | streptococcus suis is a zoonotic pathogen that causes invasive infections in humans and pigs. it has been reported that s. suis infection in humans is mostly caused by serotype 2. however, human cases caused by other serotypes have rarely been reported. this is the first report of a human case of infection with s. suis serotype 31 in thailand. | 2015 | 26420029 |
| characterization of five zoonotic streptococcus suis strains from germany, including one isolate from a recent fatal case of streptococcal toxic shock-like syndrome in a hunter. | a streptococcus suis isolate from a german hunter with streptococcal toxic shock-like syndrome (stsls) and four additional zoonotic isolates were genotyped as mrp(+) epf* (variant 1890) sly(+) cps2(+). all five zoonotic german strains were characterized by high multiplication in human blood samples ex vivo, but induction of only low levels of proinflammatory cytokines compared to a chinese stsls strain. | 2015 | 26424844 |
| streptococcus suis in employees and the environment of swine slaughterhouses in são paulo, brazil: occurrence, risk factors, serotype distribution, and antimicrobial susceptibility. | streptococcus suis is an important pathogen in the swine industry. this article is the first to report the occurrence, risk factors, serotype distribution, and antimicrobial susceptibility of s. suis recovered from employees and environmental samples of swine slaughterhouses in brazil. tonsillar swabs from all 139 pig-slaughtering employees and 261 environmental swabs were collected for detection of s. suis and serotyping by monoplex and multiplex polymerase chain reaction, respectively. antimic ... | 2015 | 26424907 |
| clonal distribution of streptococcus suis isolated from diseased pigs in the central region of chile. | the characteristics of 29 chilean field strains of streptococcus suis recovered between 2007 and 2011 from pigs with clinical signs at different farms were studied. serotyping with use of the coagglutination test revealed that all but 1 strain belonged to serotype 6; the remaining strain was serotype 22. all the serotype-6 strains were suilysin (hemolysin)-negative; in addition, they were found to be genotypically homogeneous by enterobacterial repetitive intergenic consensus sequence-based poly ... | 2015 | 26424917 |
| antibacterial effect of porcine ptx3 against streptococcus suis type 2 infection. | pentraxin 3 (ptx3), a soluble pattern recognition receptor, plays an important role in innate immunity and has been implicated to be a candidate resistance gene against streptococcus suis 2 infection. to discover the antibacterial effect of porcine ptx3 against s. suis 2, the 42-kda ptx3 protein was expressed by chinese hamster ovary cells (cho), and an additional eukaryotic expression vector pvax-ptx3 was constructed. the expressed porcine ptx3 mediated a range of antibacterial activities inclu ... | 2015 | 26427882 |
| third case of streptococcus suis infection in greece. | streptococcus suis is a facultative anaerobic, gram-positive coccus that can cause severe disease to both pigs and humans. its zoonotic potential was first recognized in 1968 when the first human case of meningitis was reported in denmark. since then, over 1600 human cases have been reported worldwide, the vast majority of which originated in southeast asia, and, thus, s. suis has been fairly characterized as an emerging pathogen. infection in humans presents most commonly as bacteremia and/or m ... | 2015 | 26435860 |
| interaction of fibrinogen and muramidase-released protein promotes the development of streptococcus suis meningitis. | muramidase-released protein (mrp) is as an important virulence marker of streptococcus suis (s. suis) serotype 2. our previous works have shown that mrp can bind human fibrinogen (hfg); however, the function of this interaction in s. suis meningitis is not known. in this study, we found that the deletion of mrp significantly impairs the hfg-mediated adherence and traversal ability of s. suis across human cerebral microvascular endothelial cells (hcmec/d3). measurement of the permeability to luci ... | 2015 | 26441928 |
| emodin affects biofilm formation and expression of virulence factors in streptococcus suis atcc700794. | streptococcus suis (s. suis) is a swine pathogen and also a zoonotic agent. in this study, the effects of subinhibitory concentrations (sub-mics) of emodin on biofilm formation by s. suis atcc700794 were evaluated. as quantified by crystal violet staining, biofilm formation by s. suis atcc700794 was dose-dependently decreased after growth with 1/2 mic, 1/4 mic, or 1/8 mic of emodin. by scanning electron microscopy, the structural architecture of the s. suis atcc700794 biofilms was examined follo ... | 2015 | 26446827 |
| genomic comparison between pathogenic streptococcus agalactiae isolated from nile tilapia in thailand and fish-derived st7 strains. | streptococcus agalactiae, or group b streptococcus (gbs), is a highly virulent pathogen in aquatic animals, causing huge mortalities worldwide. in thailand, the serotype ia, β-hemolytic gbs, belonging to sequence type (st) 7 of clonal complex (cc) 7, was found to be the major cause of streptococcosis outbreaks in fish farms. in this study, we performed an in silico genomic comparison, aiming to investigate the phylogenetic relationship between the pathogenic fish strains of thai st7 and other st ... | 2015 | 26455417 |
| streptococcus suis vaccines: candidate antigens and progress. | streptococcus suis is a major swine pathogen and an emerging zoonotic agent of human meningitis and streptococcal toxic shock-like syndrome. s. suis is a well-encapsulated pathogen and multiple serotypes have been described based on the capsular polysaccharide antigenic diversity. in addition, high genotypic, phenotypic and geographic variability exits among strains within the same serotype. besides, s. suis uses an arsenal of virulence factors to evade the host immune system. together, these ch ... | 2015 | 26468755 |
| canada: distribution of streptococcus suis (from 2012 to 2014) and actinobacillus pleuropneumoniae (from 2011 to 2014) serotypes isolated from diseased pigs. | 2015 | 26483588 | |
| crystal structure and identification of two key amino acids involved in ai-2 production and biofilm formation in streptococcus suis luxs. | streptococcus suis has emerged as an important zoonotic pathogen that causes meningitis, arthritis, septicemia and even sudden death in pigs and humans. quorum sensing is the signaling network for cell-to-cell communication that bacterial cells can use to monitor their own population density through production and exchange of signal molecules. s-ribosylhomocysteinase (luxs) is the key enzyme involved in the activated methyl cycle. autoinducer 2 (ai-2) is the adduct of borate and a ribose derivat ... | 2015 | 26484864 |
| interactions of streptococcus suis serotype 2 with human meningeal cells and astrocytes. | streptococcus suis serotype 2 is an important porcine pathogen and emerging zoonotic agent responsible for meningitis, of which different sequence types predominate worldwide. though bacterial meningitis is defined as an exacerbated inflammation of the meninges, the underlying astrocytes of the glia limitans superficialis may also be implicated. however, the interactions between this pathogen and human meningeal cells or astrocytes remain unknown. furthermore, the roles of well-described virulen ... | 2015 | 26502903 |
| streptococcus suis meningitis: a systematic review and meta-analysis. | streptococcus suis is the most common cause of meningitis in pork consuming and pig rearing countries in south-east asia. we performed a systematic review of studies on s. suis meningitis to define the clinical characteristics, predisposing factors and outcome. | 2015 | 26505485 |
| a novel endolysin disrupts streptococcus suis with high efficiency. | streptococcus suis serotype 2 (s. suis 2) is a zoonotic pathogen that exhibits high-level resistance and multi-drug resistance to classic antibiotics and causes serious human casualties and heavy economic losses in the swine industry worldwide. therefore, alternative therapies or novel antibacterial agents need to be developed to combat this pathogen. a novel endolysin derived from the s. suis temperate phage phi7917, termed ly7917, was identified, which had broad lytic activity against s. suis ... | 2015 | 26534896 |
| two novel regulators of n-acetyl-galactosamine utilization pathway and distinct roles in bacterial infections. | bacterial pathogens can exploit metabolic pathways to facilitate their successful infection cycles, but little is known about roles of d-galactosamine (galn)/n-acetyl-d-galactosamine (galnac) catabolism pathway in bacterial pathogenesis. here, we report the genomic reconstruction of galn/galnac utilization pathway in streptococci and the diversified aga regulons. we delineated two new paralogous agar regulators for the galn/galnac catabolism pathway. the electrophoretic mobility shift assays exp ... | 2015 | 26540018 |
| draft genome sequences of nine streptococcus suis strains isolated in the united states. | streptococcus suis is a swine pathogen responsible for economic losses to the pig industry worldwide. additionally, it is a zoonotic agent that can cause severe infections in those in close contact with infected pigs and/or who consume uncooked or undercooked pork products. here, we report nine draft genome sequences of s. suis. | 2015 | 26543125 |
| whole genome investigation of a divergent clade of the pathogen streptococcus suis. | streptococcus suis is a major porcine and zoonotic pathogen responsible for significant economic losses in the pig industry and an increasing number of human cases. multiple isolates of s. suis show marked genomic diversity. here, we report the analysis of whole genome sequences of nine pig isolates that caused disease typical of s. suis and had phenotypic characteristics of s. suis, but their genomes were divergent from those of many other s. suis isolates. comparison of protein sequences predi ... | 2015 | 26583006 |
| hyaluronate lyase activity of streptococcus suis serotype 2 and modulatory effects of hyaluronic acid on the bacterium's virulence properties. | streptococcus suis serotype 2 is a major swine pathogen and zoonotic agent worldwide causing mainly meningitis and septicemia. hyaluronate lyases are enzymes that degrade hyaluronic acid, a major constituent of animal tissues, and have been reported as virulence factors in various bacterial species. since the hyaluronate lyase of s. suis has been considered ambiguously as a virulence factor, we screened 50 isolates from the three major clonal complexes found in north america (sequence type [st] ... | 2015 | 26611338 |
| targeting trem-1 signaling in the presence of antibiotics is effective against streptococcal toxic-shock-like syndrome (stsls) caused by streptococcus suis. | streptococcus suis (s.suis), a major swine pathogen, is also a severe threat to human health. infection with highly virulent strains of s. suis can cause human streptococcal toxic-shock-like syndrome (stsls), which is associated with high serum pro-inflammatory cytokine levels and a high mortality rate. our previous study indicated that highly virulent s. suis infection could activate the trem-1 signaling pathway, which promotes host clearance of s. suis during early infection. however, it remai ... | 2015 | 26618144 |
| identification of novel laminin- and fibronectin-binding proteins by far-western blot: capturing the adhesins of streptococcus suis type 2. | bacterial cell wall (cw) and extracellular (ec) proteins are often involved in interactions with extracellular matrix (ecm) proteins such as laminin (ln) and fibronectin (fn), which play important roles in adhesion and invasion. in this study, an efficient method combining proteomic analysis and far-western blotting assays was developed to screen directly for bacterial surface proteins with ln- and fn-binding capacity. with this approach, fifteen potential ln-binding proteins and five potential ... | 2015 | 26636044 |
| expression of a tandemly arrayed plectasin gene from pseudoplectania nigrella in pichia pastoris and its antimicrobial activity. | in recent years, various naturally occurring defence peptides such as plectasin have attracted considerable research interest because they could serve as alternatives to antibiotics. however, the production of plectasin from natural microorganisms is still not commercially feasible because of its low expression levels and weak stability. a tandemly arrayed plectasin gene (1,002 bp) from pseudoplectania nigrella was generated using the isoschizomer construction method, and was inserted into the p ... | 2016 | 26643963 |
| multiplex pcr and microarray for detection of swine respiratory pathogens. | porcine respiratory disease complex (prdc) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. no simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with prdc. furthermore, the detection of most of the bacterial pathogens implicated in prdc currently requires time-consuming culture-based methods that can take several days to obtain results. in this study, ... | 2015 | 26662640 |
| in vivo pharmacodynamics of cefquinome in a neutropenic mouse thigh model of streptococcus suis serotype 2 at varied initial inoculum sizes. | streptococcus suis serotype 2 is an emerging zoonotic pathogen and causes severe disease in both pigs and human beings. cefquinome (ceq), a fourth-generation cephalosporin, exhibits broad-spectrum activity against gram-positive bacteria such as s. suis. this study evaluated the in vitro and in vivo antimicrobial activities of ceq against four strains of s. suis serotype 2 in a murine neutropenic thigh infection model. we investigated the effect of varied inoculum sizes (10(6) to 10(8) cfu/thigh) ... | 2016 | 26666923 |
| purification and characterization of suicin 65, a novel class i type b lantibiotic produced by streptococcus suis. | bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent streptococcus suis (serotype 2). in this study, a novel bacteriocin produced by s. suis has been identified and characterized. the producing strain s. suis 65 (serotype 2) was fo ... | 2015 | 26709705 |
| prioritization of zoonotic diseases of public health significance in vietnam. | prioritization of zoonotic diseases is critical as it facilitates optimization of resources, greater understanding of zoonotic diseases and implementation of policies promoting multisectoral collaboration. this study aimed to establish strategic priorities for zoonotic diseases in vietnam taking a key stakeholder approach. | 2015 | 26719937 |
| enolase of streptococcus suis serotype 2 enhances blood-brain barrier permeability by inducing il-8 release. | streptococcus suis serotype 2 (ss2) is an emerging zoonosis, and meningitis is the most frequent clinical manifestation, but mechanism of its virulent factor, enolase (eno), is unknown in meningitis. in this study, eno was inducibly expressed and added to an in vitro transwell co-culture model of the blood-brain barrier (bbb) consisted of porcine brain microvascular endothelial cells (pbmecs) and astrocytes (acs), the results showed that eno induces a significant increase in bbb permeability and ... | 2016 | 26732390 |
| host-pathogen interactions in bacterial meningitis. | bacterial meningitis is a devastating disease occurring worldwide with up to half of the survivors left with permanent neurological sequelae. due to intrinsic properties of the meningeal pathogens and the host responses they induce, infection can cause relatively specific lesions and clinical syndromes that result from interference with the function of the affected nervous system tissue. pathogenesis is based on complex host-pathogen interactions, some of which are specific for certain bacteria, ... | 2016 | 26744349 |
| characterization of iga1 protease as a surface protective antigen of streptococcus suis serotype 2. | iga1 protease of streptococcus suis serotype 2 (ss2) has been proven to be relative with virulence and immunogenicity, however, its protective efficacy remained to be evaluated. the present study found evidence that immunization with purified recombinant iga1 protease (600-1926aa) could induce high igg antibody titers and could confer complete protection against a challenge with a lethal dose of ss2 in a mouse model. in addition, our findings confirmed that the iga1 protease distributes on the s ... | 2016 | 26774332 |
| further identification of novel lantibiotic operons using lanm-based genome mining. | lantibiotics are gene-encoded antimicrobial peptides that are distinguished by the presence of the unusual structures, lanthionine and β-methyllanthionine, which are introduced through enzyme-catalysed post-translational modification. lantibiotics can be subdivided on the basis of the nature of the enzyme(s) which catalyse this reaction. lantibiotic synthetases, generically designated lanm, which catalyse the dehydration of serines (and threonines) followed by the formation lanthionine (and β-me ... | 2011 | 26781497 |
| a novel virulence-associated protein, vape, in streptococcus suis serotype 2. | streptococcus suis serotype 2 (ss2) is an important pathogen that affects pigs. however, neither its virulence nor its pathogenesis of infection has yet to be fully elucidated. the present study identifies a novel virulence‑associated protein e gene (vape) of ss2. to investigate the importance of vape in ss2 infection, a vape knock‑out mutant based on ss2 wild‑type strain zy458 was designated 458δvape. 458δvape was generated through homologous recombination, using a combined plasmid with a vape ... | 2016 | 26821177 |
| [streptococcal toxic shock-like syndrome due to streptococcus suis serotype 2 in a japanese pig farmer]. | streptococcus suis is a major swine pathogen. it has recently been recognized as an emerging zoonosis that causes mainly meningitis and sepsis in human. in particular, toxic shock-like syndrome (tsls) caused by this pathogen has a high mortality rate. however, misidentification of s. suis by conventional biochemical and commercial identification tests is not rare. the patient was a 71-year-old man who worked as a pig farmer who was admitted for fever, oliguria and subcutaneous hemorrhage. he was ... | 2015 | 26821523 |
| screening of virulence-associated genes as a molecular typing method for characterization of streptococcus suis isolates recovered from wild boars and pigs. | streptococcus suis is an important zoonotic pathogen associated with a wide range of diseases in pigs, but has also been isolated from wild animals such as rabbits and wild boars. in the current study, 126 s. suis isolates recovered from pigs (n = 85) and wild boars (n = 41) were tested by polymerase chain reaction (pcr) for the presence of nine virulence-associated genes. s. suis isolates from wild boars were differentiated by the lower detection rates of the epf, sly, mrp, sao and dlta genes ( ... | 2016 | 26831161 |
| a novel inhibition elisa for the detection and monitoring of penicillium marneffei antigen in human serum. | the thermally dimorphic fungus penicillium marneffei is a causative agent of penicilliosis marneffei, a disease considered to be an acquired immune deficiency syndrome (aids)-defining illness in southeast asia and southern china. we have developed an inhibition enzyme-linked immunosorbent assay (inh-elisa) incorporating the yeast phase specific mannoprotein-binding monoclonal antibody 4d1 for the detection of p. marneffei infection. in our sample set, the test detected antigenemia in all 45 (100 ... | 2016 | 26838686 |
| identification and characterization of igde, a novel igg-degrading protease of streptococcus suis with unique specificity for porcine igg. | streptococcus suisis a major endemic pathogen of pigs causing meningitis, arthritis, and other diseases. zoonotics. suisinfections are emerging in humans causing similar pathologies as well as severe conditions such as toxic shock-like syndrome. recently, we discovered an ides family protease ofs. suisthat exclusively cleaves porcine igm and represents the first virulence factor described, linkings. suisto pigs as their natural host. here we report the identification and characterization of a no ... | 2016 | 26861873 |
| comparative genomic analysis of the icesa2603 family ices and spread of erm(b)- and tet(o)-carrying transferable 89k-subtype ices in swine and bovine isolates in china. | integrative and conjugative elements (ices) of the icesa2603 family have been isolated from several species of streptococcus spp.; however, the comparative genomic and evolutionary analyses of these particular ices are currently only at their initial stages. by investigating 13 ices of the icesa2603 family and two icesa2603 family-like ices derived from diverse hosts and locations, we have determined that ices comprised a backbone of 30 identical syntenic core genes and accessory genes that were ... | 2016 | 26870017 |
| neutrophil extracellular taps play an important role in clearance of streptococcus suis in vivo. | streptococcus suis infection induces formation of neutrophil extracellular traps (nets) in vitro; however, the contribution of nets-mediated killing to the pathogenesis of s. suis in vivo is yet to be elicited. the findings of the present study indicated that extracellular dna fiber can be induced in a murine model in response to s. suis infection. a nuclease that destroys their structure was used to evaluate the role of nets on s. suis infection. treatment with nuclease resulted in a greater ba ... | 2016 | 26876770 |
| the cody regulator is essential for virulence in streptococcus suis serotype 2. | the main role of cody, a global regulatory protein in most low g + c gram-positive bacteria, is in transcriptional repression. to study the functions of cody in streptococcus suis serotype 2 (s. suis 2), a mutant cody clone named ∆cody was constructed to explore the phenotypic variation between ∆cody and the wild-type strain. the result showed that the cody mutation significantly inhibited cell growth, adherence and invasion ability of s. suis 2 to hep-2 cells. the cody mutation led to decreased ... | 2016 | 26883762 |
| a hypothetical model of host-pathogen interaction of streptococcus suis in the gastro-intestinal tract. | streptococcus suis (ss) is a zoonotic pathogen that can cause systemic infection in pigs and humans. the ingestion of contaminated pig meat is a well-established risk factor for zoonotic s. suis disease. in our studies, we provide experimental evidence that s. suis is capable to translocate across the host gastro-intestinal tract (git) using in vivo and in vitro models. hence, s. suis should be considered an emerging foodborne pathogen. in this addendum, we give an overview of the complex intera ... | 2016 | 26900998 |
| explaining the serological characteristics of streptococcus suis serotypes 1 and 1/2 from their capsular polysaccharide structure and biosynthesis. | the capsular polysaccharide (cps) is a major virulence factor in many encapsulated pathogens, as it is the case for streptococcus suis, an important swine pathogen and emerging zoonotic agent. moreover, the cps is the antigen at the origin of s. suis classification into serotypes. hence, analyses of the cps structure are an essential step to dissect its role in virulence and the serological relations between important serotypes. here, the cpss of serotypes 1 and 1/2 were purified and characteriz ... | 2016 | 26912653 |
| preparation, characterization and pharmacokinetics of doxycycline hydrochloride and florfenicol polyvinylpyrroliddone microparticle entrapped with hydroxypropyl-β-cyclodextrin inclusion complexes suspension. | in order to effectively control the bacterial pneumonia in pigs, doxycycline hydrochloride (doxhcl) and florfenicol (ff) microparticle suspension together with inclusion complexes was prepared by using hydroxypropyl-β-cyclodextrin (hp-β-cd) as host molecules, polyvinylpyrroliddone (pvp) as polymer carriers and hydroxypropyl methyl cellulose (hpmc) as suspending agents. in vitro antibacterial activity, properties, stability and pharmacokinetics of the suspension were studied. the results demonstr ... | 2016 | 26918512 |
| development of a multiplex pcr assay to detect the major clonal complexes of streptococcus suis relevant to human infection. | multilocus sequence typing (mlst) is considered a reliable method for providing insight into the streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. indeed, mlst has revealed the presence of several clonal complexes (ccs) within the streptococcus suis population. however, the method is costly, time-consuming and difficult to use for screening large numbers of isolates. in this study, a multiplex pcr assay was developed to identif ... | 2016 | 26932590 |
| effect of the glycosyltransferases on the capsular polysaccharide synthesis of streptococcus suis serotype 2. | streptococcus suis serotype 2 (s. suis 2) is a serious zoonotic pathogen causing septicemia and meningitis in piglets and humans. the capsular polysaccharide (cps) is an essential virulence factor for s. suis 2 to infect the host. the synthesis of cps repeating units involves multiple glycosyltransferases. in this study, four genes (cps2e, cps2g, cps2j and cps2l) encoding different glycosyltransferases involved in cps synthesis were researched in s. suis 2. four deletion mutants (δcps2e, δcps2g, ... | 2016 | 26946377 |
| population structure and antimicrobial resistance profiles of streptococcus suis serotype 2 sequence type 25 strains. | strains of serotype 2 streptococcus suis are responsible for swine and human infections. different serotype 2 genetic backgrounds have been defined using multilocus sequence typing (mlst). however, little is known about the genetic diversity within each mlst sequence type (st). here, we used whole-genome sequencing to test the hypothesis that s. suis serotype 2 strains of the st25 lineage are genetically heterogeneous. we evaluated 51 serotype 2 st25 s. suis strains isolated from diseased pigs a ... | 2016 | 26954687 |
| the role of porcine monocyte derived dendritic cells (modc) in the inflammation storm caused by streptococcus suis serotype 2 infection. | streptococcus suis is an important swine pathogen and zoonotic agent. infection with this highly pathogenic strain can cause streptococcal toxic shock-like syndrome (stsls), characterized by a th-1 inflammatory cytokine storm, and a high mortality rate. monocyte derived dendritic cells (modcs) are known to stimulate th-1 cell differentiation, but the role of modcs in stsls remains to be elucidated. | 2016 | 26974437 |
| streptococcus suis in invasive human infections in poland: clonality and determinants of virulence and antimicrobial resistance. | the purpose of this study was to perform an analysis of streptococcus suis human invasive isolates, collected in poland by the national reference centre for bacterial meningitis. isolates obtained from 21 patients during 2000-2013 were investigated by phenotypic tests, multilocus sequence typing (mlst), analysis of the tr9 locus from the multilocus variable number tandem repeat (vntr) analysis (mlva) scheme and pulsed-field gel electrophoresis (pfge) of smai-digested dna. determinants of virulen ... | 2016 | 26980093 |
| a zebrafish larval model to assess virulence of porcine streptococcus suis strains. | streptococcus suis is an encapsulated gram-positive bacterium, and the leading cause of sepsis and meningitis in young pigs resulting in considerable economic losses in the porcine industry. it is also considered an emerging zoonotic agent. in the environment, both avirulent and virulent strains occur in pigs, and virulent strains appear to cause disease in both humans and pigs. there is a need for a convenient, reliable and standardized animal model to assess s. suis virulence. a zebrafish (dan ... | 2016 | 26999052 |
| first case in spain of septic shock by streptococcus suis possible food-borne. | 2016 | 27021908 | |
| structural insight for substrate tolerance to 2-deoxyribose-5-phosphate aldolase from the pathogen streptococcus suis. | 2-deoxyribose-5-phosphate aldolase (dera) is a class i aldolase that catalyzes aldol condensation of two aldehydes in the active site, which is particularly germane in drug manufacture. structural and biochemical studies have shown that the active site of dera is typically loosely packed and displays broader substrate specificity despite sharing conserved folding architecture with other aldolases. the most distinctive structural feature of dera compared to other aldolases is short and flexible c ... | 2016 | 27033207 |
| species-specific real-time pcr assay for the detection of streptococcus suis from clinical specimens. | a real-time polymerase chain reaction was developed to detect all known strains of streptococcus suis. the assay was highly specific, and sensitivity was <10 copies/assay for s. suis detection from clinical samples. | 2016 | 27041105 |
| sub-mic tylosin inhibits streptococcus suis biofilm formation and results in differential protein expression. | streptococcus suis (s.suis) is an important zoonotic pathogen that causes severe diseases in humans and pigs. biofilms of s. suis can induce persistent infections that are difficult to treat. in this study, the effect of tylosin on biofilm formation of s. suis was investigated. 1/2 minimal inhibitory concentration (mic) and 1/4 mic of tylosin were shown to inhibit s. suis biofilm formation in vitro. by using the itraq strategy, we compared the protein expression profiles of s. suis grown with su ... | 2016 | 27065957 |
| a streptococcus suis lysm domain surface protein contributes to bacterial virulence. | streptococcus suis (ss) is a major swine pathogen, as well as a zoonotic agent for humans. numerous factors contribute to ss virulence, but the pathogenesis of ss infection is poorly understood. here, we show that a novel ss surface protein containing a lysm at the n-terminus (ss9-lysm) contributes to ss virulence. homology analysis revealed that the amino acid sequence of ss9-lysm from the ss strain gz0565 shares 99.8-68.7% identity with homologous proteins from other ss strains and 41.2% ident ... | 2016 | 27066710 |
| screening for antibacterial and antioxidant activities and phytochemical analysis of oroxylum indicum fruit extracts. | oroxylum indicum, which is called pheka in thai, is a traditional thai plant in the bignoniaceae family with various ethnomedical uses such as as an astringent, an anti-inflammatory agent, an anti-bronchitic agent, an anti-helminthic agent and an anti-microbial agent. the young fruits of this plant have also been consumed as vegetables. however, there has been no report concerning its antibacterial activities, especially activities related to clinically isolated pathogenic bacteria and the in vi ... | 2016 | 27070560 |
| sbp2 plays an important role in the virulence changes of different artificial mutants of streptococcus suis. | streptococcus suis (ss) is an important bacterial zoonotic pathogen, which can cause infections in pigs and humans. however, the pathogenesis of this bacterium remains unclear, even though some putative virulence factors (vfs) have been reported. comparative proteomics could be used to identify markers that can distinguish bacterial strains with different virulence; however, the application of this method is restricted by the genome diversities existing in different strains. in this study, two m ... | 2016 | 27077729 |
| structural basis of the interaction between the meningitis pathogen streptococcus suis adhesin fhb and its human receptor. | the recently identified streptococcus suis adhesin factor h-binding protein (fhb) targets the host cellular receptor glycolipid gbo3 through its n terminus. however, it is unclear how fhb interacts with its receptor. here, we determined the complex structure of factor h-binding protein receptor-binding domain (fhb rbd) with gb2, an analog of its receptor, revealing that gb2 binds in a pocket of the β sandwich core domain. we identified the key residues for fhb rbd receptor binding using mutagene ... | 2016 | 27086582 |
| monitoring of antimicrobial susceptibility of respiratory tract pathogens isolated from diseased cattle and pigs across europe, 2009-2012: vetpath results. | vetpath is an ongoing pan-european antibiotic susceptibility monitoring programme that collects pathogens from diseased cattle, pigs and poultry. in the current study, 996 isolates from cattle and pig respiratory tract infections were tested for their antimicrobial susceptibilities. non-replicate lung samples or nasopharyngeal/nasal swabs were collected from animals with acute clinical signs in 10 countries during 2009-2012. pasteurella multocida, mannheimia haemolytica and histophilus somni fro ... | 2016 | 27102206 |
| impact of sub-inhibitory concentrations of amoxicillin on streptococcus suis capsule gene expression and inflammatory potential. | streptococcus suis is an important swine pathogen and emerging zoonotic agent worldwide causing meningitis, endocarditis, arthritis and septicemia. among the 29 serotypes identified to date, serotype 2 is mostly isolated from diseased pigs. although several virulence mechanisms have been characterized in s. suis, the pathogenesis of s. suis infections remains only partially understood. this study focuses on the response of s. suis p1/7 to sub-inhibitory concentrations of amoxicillin. first, caps ... | 2016 | 27104570 |
| protection against streptococcus suis serotype 2 infection using a capsular polysaccharide glycoconjugate vaccine. | streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. despite decades of research for an efficient vaccine, none is currently available. based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was selected to elicit opsonizing anti-capsular polysaccharide (anti-cps) igg antibodies. in this work, glycoconjugate prototypes were prepared by coupling s. suis type 2 cps to tetanu ... | 2016 | 27113360 |
| genetic diversity of streptococcus suis serotype 2 isolated from pigs in brazil. | streptococcus suis is an emerging zoonotic pathogen that causes septicemia, meningitis, arthritis, and pneumonia in swine and humans. the present study aimed to characterize the genetic diversity of s. suis serotype 2 isolated from pigs showing signs of illness in brazil using pulsed-field gel electrophoresis (pfge), single-enzyme amplified fragment length polymorphism (se-aflp), and profiling of virulence-associated markers. a total of 110 isolates were studied, 62.7% of which were isolated fro ... | 2016 | 27127337 |
| monitoring of antimicrobial susceptibility of streptococcus suis in the netherlands, 2013-2015. | the objective of the present study was to analyse the in vitro antimicrobial susceptibility of streptococcus suis isolates from post-mortem samples from pigs in the netherlands. s. suis isolates originated from diagnostic submissions of pigs sent to the pathology department of gd animal health, from april 2013 till june 2015. minimal inhibitory concentrations (mics) of in total 15 antimicrobials were assessed by broth microdilution following clsi recommendations. mic50 and mic90 values were dete ... | 2016 | 27131836 |
| gida, a trna modification enzyme, contributes to the growth, and virulence of streptococcus suis serotype 2. | glucose-inhibited division protein (gida), is a trna modification enzyme functioning together with mnme in the addition of a carboxymethylaminomethyl group to position 5 of the anticodon wobble uridine of trna. here, we report a gida homolog from a chinese isolate sc-19 of the zoonotic streptococcus suis serotype 2 (ss2). gida disruption led to a defective growth, increased capsule thickness, and reduced hemolytic activity. moreover, the gida deletion mutant (δgida) displayed reduced mortality a ... | 2016 | 27148493 |
| metabolic context of the competence-induced checkpoint for cell replication in streptococcus suis. | natural genetic transformation is a transient, rapidly progressing energy-consuming process characterized by expression of the transformasome and competence-associated regulatory genes. this transient state is tightly controlled to avoid potentially adverse effects of genetic recombination on genome integrity during cell division. we investigated the global response of streptococcus suis to exposure to the sigx competence-inducing peptide (xip), and thus to the activation of the competence machi ... | 2016 | 27149631 |
| impact of serotype and sequence type on the preferential aerosolization of streptococcus suis. | streptococcus suis is a swine pathogen that causes pneumonia, septicemia and meningitis. it is also an important zoonotic agent responsible of several outbreaks in china. s. suis strains are classified into 35 serotypes based on the composition of their polysaccharide capsule. s. suis serotype 2 causes the majority of severe infections in pigs and in human, and can be further subdivided into sequence types (sts) based on multilocus sequence typing. the st1 is associated with highly virulent stra ... | 2016 | 27180230 |
| loss of 89k pathogenicity island in epidemic streptococcus suis, china. | 2016 | 27192444 | |
| transcriptional analysis of prrsv-infected porcine dendritic cell response to streptococcus suis infection reveals up-regulation of inflammatory-related genes expression. | the porcine reproductive and respiratory syndrome virus (prrsv) is one of the most important swine pathogens and often serves as an entry door for other viral or bacterial pathogens, of which streptococcus suis is one of the most common. pre-infection with prrsv leads to exacerbated disease caused by s. suis infection. very few studies have assessed the immunological mechanisms underlying this higher susceptibility. since antigen presenting cells play a major role in the initiation of the immune ... | 2016 | 27213692 |
| functional definition of bira suggests a biotin utilization pathway in the zoonotic pathogen streptococcus suis. | biotin protein ligase is universal in three domains of life. the paradigm version of bpl is the escherichia coli bira that is also a repressor for the biotin biosynthesis pathway. streptococcus suis, a leading bacterial agent for swine diseases, seems to be an increasingly-important opportunistic human pathogen. unlike the scenario in e. coli, s. suis lacks the de novo biotin biosynthesis pathway. in contrast, it retains a bioy, a biotin transporter-encoding gene, indicating an alternative survi ... | 2016 | 27217336 |
| chemical constituents from campylotropis hirtella. | a phytochemical investigation on the roots of campylotropis hirtella afforded nine new isoflavones (3-9, 12, 15), two new isoflavans (10 and 11), one new coumestan (1), and three new prenylated benzoic acid derivatives (2, 13, 14), together with twenty-four known compounds. their structures were established by spectroscopic analysis and circular dichroism data. the isolated compounds were also evaluated for their antibacterial activities against enterococcus faecalis, salmonella gallinarum, stre ... | 2016 | 27220079 |
| efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions. | streptococci may colonize the epithelium in the airways and other entry sites. while local infection often remains asymptomatic, severe or even fatal diseases occur when streptococci become invasive and spread to different sites in the infected host. we have established porcine respiratory air-liquid interface cultures (ali) from the porcine lung to analyze the interaction of streptococci with their primary target cells. as representative of the streptococcal family we chose streptococcus suis ( ... | 2016 | 27229328 |
| binding of human fibrinogen to mrp enhances streptococcus suis survival in host blood in a αxβ2 integrin-dependent manner. | the gram-positive bacterium streptococcus suis serotype 2 (s. suis 2), an important zoonotic pathogen, induces strong systemic infections in humans; sepsis and meningitis are the most common clinical manifestations and are often accompanied by bacteremia. however, the mechanisms of s. suis 2 survival in human blood are not well understood. in our previous study, we identified muramidase-released protein (mrp), a novel human fibrinogen (hfg)-binding protein (fbp) in s. suis 2 that is an important ... | 2016 | 27231021 |
| effects of dietary fibre source on microbiota composition in the large intestine of suckling piglets. | this study aimed to investigate the effects of dietary fibre sources on the gut microbiota in suckling piglets, and to test the hypothesis that a moderate increase of dietary fibre may affect the gut microbiota during the suckling period. suckling piglets were fed different fibre-containing diets or a control diet from postnatal day 7 to 22. digesta samples from cecum, proximal colon and distal colon were used for pig intestinal tract chip analysis. the data showed that the effects of fibre-cont ... | 2016 | 27231242 |
| the roles of rela/(p)ppgpp in glucose-starvation induced adaptive response in the zoonotic streptococcus suis. | the (p)ppgpp signal molecules play a central role in the stringent response (sr) to adapt to nutrient starvation in bacteria, yet the carbohydrate starvation induced adaptive response and the roles of sr in this response is not well characterized, especially in gram-positives. here, two (p)ppgpp synthetases rela and relq are identified in streptococcus suis, an important emerging zoonotic gram-positive bacterium, while only rela is functional under glucose starvation. to characterize the roles o ... | 2016 | 27255540 |
| a novel suicide shuttle plasmid for streptococcus suis serotype 2 and streptococcus equi ssp. zooepidemicus gene mutation. | the mariner-based himar1 system has been utilized for creating mutant libraries of many gram-positive bacteria. streptococcus suis serotype 2 (ss2) and streptococcus equi ssp. zooepidemicus (sez) are primary pathogens of swine that threaten the swine industry in china. to provide a forward-genetics technology for finding virulent phenotype-related genes in these two pathogens, we constructed a novel temperature-sensitive suicide shuttle plasmid, pmar4s, which contains the himar1 system transposo ... | 2016 | 27256117 |
| subtilisin-like protease-1 secreted through type iv secretion system contributes to high virulence of streptococcus suis 2. | streptococcus suis serotype 2 is an emerging zoonotic pathogen that triggered two outbreaks of streptococcal toxic shock syndrome (stss) in china. our previous research demonstrated that a type iv secretion system (t4ss) harbored in the 89k pathogenicity island contributes to the pathogenicity of s. suis 2. in the present study, a shotgun proteomics approach was employed to identify the effectors secreted by t4ss in s. suis 2, and surface-associated subtilisin-like protease-1 (sspa-1) was identi ... | 2016 | 27270879 |
| antimicrobial activity of penicillin g and n-acetylcystein on planktonic and sessile cells of streptococcus suis. | the aim of this study was to investigate the capacity of streptococcus suis strains to form biofilms and to evaluate the antimicrobial activity of penicillin g and n-acetylcystein (nac) on both s. suis sessile and planktonic forms. only non-typeable isolates of s. suis were correlated with a greater biofilm formation capacity. the mci of penicillin g and nac required for inhibiting biofilm growth were higher than the required concentration for inhibiting planktonic growth. the combinations of na ... | 2016 | 27282001 |
| astrocytes enhance streptococcus suis-glial cell interaction in primary astrocyte-microglial cell co-cultures. | streptococcus (s.) suis infections are the most common cause of meningitis in pigs. moreover, s. suis is a zoonotic pathogen, which can lead to meningitis in humans, mainly in adults. we assume that glial cells may play a crucial role in host-pathogen interactions during s. suis infection of the central nervous system. glial cells are considered to possess important functions during inflammation and injury of the brain in bacterial meningitis. in the present study, we established primary astrocy ... | 2016 | 27304968 |
| identification of streptococcus suis meningitis through population-based surveillance, togo, 2010-2014. | during 2010-2014, we enrolled 511 patients with suspected bacterial meningitis into surveillance in 2 districts of northern togo. we identified 15 persons with streptococcus suis infection; 10 had occupational contact with pigs, and 12 suffered neurologic sequelae. s. suis testing should be considered in rural areas of the african meningitis belt. | 2016 | 27314251 |
| contribution of nadh oxidase to oxidative stress tolerance and virulence of streptococcus suis serotype 2. | streptococcus suis is a major swine and zoonotic pathogen that causes severe infections. previously, we identified 2 spx regulators in s. suis, and demonstrated that spxa1 affects oxidative stress tolerance and virulence. however, the mechanism behind spxa1 function remains unclear. in this study, we targeted 4 genes that were expressed at significantly reduced levels in the spxa1 mutant, to determine their specific roles in adaptation to oxidative stress and virulence potential. the δnox strain ... | 2017 | 27315343 |
| mechanisms of host-pathogen protein complex formation and bacterial immune evasion of streptococcus suis protein fhb. | streptococcus suis serotype 2 (s. suis 2)-induced sepsis and meningitis are often accompanied by bacteremia. the evasion of polymorphonuclear leukocyte-mediated phagocytic clearance is central to the establishment of bacteremia caused by s. suis 2 and is facilitated by the ability of factor h (fh)-binding protein (fhb) to bind fh on the bacterial surface, thereby impeding alternative pathway complement activation and phagocytic clearance. here, c3b/c3d was found to bind to fhb, along with fh, fo ... | 2016 | 27342778 |
| current taxonomical situation of streptococcus suis. | streptococcus suis, a major porcine pathogen and an important zoonotic agent, is considered to be composed of phenotypically and genetically diverse strains. however, recent studies reported several "s. suis-like strains" that were identified as s. suis by commonly used methods for the identification of this bacterium, but were regarded as distinct species from s. suis according to the standards of several taxonomic analyses. furthermore, it has been suggested that some s. suis-like strains can ... | 2016 | 27348006 |
| auxotrophic actinobacillus pleurpneumoniae grows in multispecies biofilms without the need for nicotinamide-adenine dinucleotide (nad) supplementation. | actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, which causes important worldwide economic losses in the swine industry. several respiratory tract infections are associated with biofilm formation, and a. pleuropneumoniae has the ability to form biofilms in vitro. biofilms are structured communities of bacterial cells enclosed in a self-produced polymer matrix that are attached to an abiotic or biotic surface. virtually all bacteria can grow as a biofi ... | 2016 | 27349384 |
| live streptococcus suis type 5 strain xs045 provides cross-protection against infection by strains of types 2 and 9. | streptococcus suis is one of the common pathogens causing diseases in pigs and covers 35 serotypes with the type 2 strains being more pathogenic and zoonotic. existing inactivated or subunit vaccines, in clinical use or under trial, could not provide cross protection against other serotypes. we identified a natural low-virulence s. suis type 5 strain xs045 as a live vaccine candidate because it is highly adhesive to the cultured hep-2 cells, but with no apparent pathogenicity in mice and piglets ... | 2016 | 27349838 |
| biological activities of suilysin: role in streptococcus suis pathogenesis. | streptococcus suis is an important swine and zoonotic pathogen equipped with several virulence factors. the pore-forming toxins are the most abundant bacterial toxins and classified as critical virulence (associated) factors of several pathogens. the role of suilysin (sly), a pore-forming cholesterol-dependent cytolysin of s. suis, as a true virulence factor is under debate. most of the bacterial toxins have been reported to modulate the host immune system to facilitate invasion and subsequent r ... | 2016 | 27357518 |
| [isolation and characterization of a streptococcus suis serotype 9 from a wild cat]. | streptococcus suis (s. suis) is an emerging zoonotic pathogenic bacterium capable of infecting piglets and human and with sporadic infections in a variety of mammalian species. the aim of this study is to investigate the prevalence of s. suis in wild cats. | 2016 | 27373075 |
| simultaneous quantification and differentiation of streptococcus suis serotypes 2 and 9 by quantitative real-time pcr, evaluated in tonsillar and nasal samples of pigs. | invasive streptococcus suis (s. suis) infections in pigs are often associated with serotypes 2 and 9. mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. to unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. a quantitative real-time pcr (qpcr) targeting cps2j (serotypes 2 and 1/2) and cps9h (serotype 9) was evaluated with nasal and tonsillar samples from s. suis expos ... | 2016 | 27376336 |
| increase of β2-integrin on adhesion of thp-1 cells to collagen vitrigel membrane. | when human monocyte-derived leukemia (thp-1) cells, which are floating cells, are stimulated with lipid peroxides, or streptococcus suis, these cells adhere to a plastic plate or endothelial cells. however, it is unclear whether or not non-stimulated thp-1 cells adhere to collagen vitrigel membrane (cvm). in this study, firstly, we investigated the rate of adhesion of thp-1 cells to cvm. when thp-1 cells were not stimulated, the rate of adhesion to cvm was high. then, to identify adhesion molecu ... | 2016 | 27379801 |
| an emerging zoonotic clone in the netherlands provides clues to virulence and zoonotic potential of streptococcus suis. | streptococcus suis is a zoonotic swine pathogen and a major public health concern in asia, where it emerged as an important cause of bacterial meningitis in adults. while associated with food-borne transmission in asia, zoonotic s. suis infections are mainly occupational hazards elsewhere. to identify genomic differences that can explain zoonotic potential, we compared whole genomes of 98 s. suis isolates from human patients and pigs with invasive disease in the netherlands, and validated our ob ... | 2016 | 27381348 |
| recruitment of factor h to the streptococcus suis cell surface is multifactorial. | streptococcus suis is an important bacterial swine pathogen and a zoonotic agent. recently, two surface proteins of s. suis, fhb and fhbp, have been described for their capacity to bind factor h-a soluble complement regulatory protein that protects host cells from complement-mediated damages. results obtained in this study showed an important role of host factor h in the adhesion of s. suis to epithelial and endothelial cells. both fhb and fhbp play, to a certain extent, a role in such increased ... | 2016 | 27399785 |
| virulence studies of different sequence types and geographical origins of streptococcus suis serotype 2 in a mouse model of infection. | multilocus sequence typing previously identified three predominant sequence types (sts) of streptococcus suis serotype 2: st1 strains predominate in eurasia while north american (na) strains are generally st25 and st28. however, st25/st28 and st1 strains have also been isolated in asia and na, respectively. using a well-standardized mouse model of infection, the virulence of strains belonging to different sts and different geographical origins was evaluated. results demonstrated that although a ... | 2016 | 27409640 |
| structure determination of streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis. | streptococcus suis serotype 9 is the most prevalent s. suis serotype in several european countries. in spite of its pathogenicity for pigs and increasing zoonotic potential, limited information is available on this serotype. here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (cps), a major bacterial virulence factor and the antigen at the origin of s. suis classification into serotypes. chemical and spectroscopic data gave the repea ... | 2016 | 27423880 |