Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| tooth colored dental restorative materials: porosities and surface topography in relation to bacterial adhesion. | the present investigation was carried out to determine whether differences in initial bacterial accumulation on dental restorations could be explained by differences in surface topography, particularly porosities, of the materials. a point counting method was used to determine the number and the size of the porosities in 15 materials. the results demonstrated great variation among the materials in the number of porosities. however, in vitro bacterial adhesion tests failed to show any relationshi ... | 1982 | 6954828 |
| saliva-induced aggregation of streptococci. solubilization of a bacterial surface receptor. | 1982 | 6956448 | |
| inhibition of neutrophil chemotaxis by soluble bacterial products. | bacterial-neutrophil interactions may be critical determinants of virulence in periodontal diseases. this study was undertaken to examine the ability of major bacterial species from the human oral cavity to inhibit (1) peripheral blood neutrophil chemotaxis, (2) chemotactic formylmethionyl peptide binding, and (3) phagocytosis. included were cultured supernatants and sonic extracts obtained from strains of capnocytophaga, bacteroides gingivalis, fusobacterium nucleatum, bacteroides asaccharolyti ... | 1982 | 6956714 |
| streptococcus mutans in plaque and saliva after mouthrinsing with snf2. | mouthrinses with snf2 in 11 subjects significantly reduced (p less than 0.01) the total colony forming units (cfu) count and the numbers of streptococcus mutans and streptococcus sanguis in plaque. the numbers of s. mutans and s. sanguis were significantly more reduced than was the total cfu count. after rinsing with snf2s. sanguis was present in 97% and s. mutans in only 42% of plaque samples from tooth surfaces where they were detected after rinsing with water. snf2 also significantly reduced ... | 1982 | 6957968 |
| production of acids in rat dental plaque with or without streptococcus mutans. | 1982 | 6958370 | |
| minimum inhibitory concentration of surfactants for plaque antiadherents. | 1982 | 6959696 | |
| adherence of streptococcus sanguis to salivary mucin bound to glass. | this study demonstrated that human submandibular-sublingual saliva (hsmsl) provided a better substrate than did whole saliva or parotid saliva for the binding of streptococcus sanguis in a glass adherence assay. additional evidence indicated that the lower molecular weight salivary mucin in hsmsl was involved in these interactions. mucin's sialic acid residues were found to play a major role in mediating the binding of certain strains of streptococcus sanguis. | 1982 | 6960040 |
| colonization and cariogenic potential in hamsters of the bacterium streptococcus sanguis isolated from human dental plaque. | strains of strep. sanguis, freshly isolated from human dental plaque, were successfully implanted into albino hamsters. transmission of the organisms from infected to uninfected animals occurred naturally. the transfer was as effective between unrelated hamsters as between dams and their offspring. three of the strains tested did not cause caries in hamsters. laboratory strains of strep. sanguis did not colonize the hamsters. two morphological variants of strep. sanguis with different abilities ... | 1982 | 6961894 |
| effect of extracellular polysaccharides on diffusion of naf and [14c]-sucrose in human dental plaque and in sediments of the bacterium streptococcus sanguis 804 (nctc 10904). | it has been postulated that extracellular polysaccharides form a barrier to diffusion in dental plaque. diffusion coefficients, d, were measured for naf and [14c]-sucrose in glucan-free and glucan-containing sediments of strep. sanguis 804 at 37 degrees c. there was a tendency for naf and [14c]-sucrose to diffuse faster as the carbohydrate concentration in the sediments increased. naf diffused only 38 per cent more slowly in cell-free glucan sediment than in water, suggesting that glucan per se ... | 1982 | 6961914 |
| [on the external action of fluorides: their antibacterial and plaque-inhibiting significance. i: literature survey and in vitro studies]. | 1982 | 6963802 | |
| microbial studies on plaque from carious and caries-free proximal tooth surfaces in a population with high caries experience. | 1981 | 6973394 | |
| regulatory effect of monocytes on t cell proliferative responses to oral microbial antigens. | mononuclear cell preparations isolated by ficoll-hypaque centrifugation from human peripheral blood were found to vary considerably in the number of monocytes they contained (mean, 20.3%; range, 13 to 33%). the regulatory role of monocytes in t cell proliferative responses to sonic extracts of a panel of oral microorganisms was therefore investigated. t cells were fractionated by anti-immunoglobulin chromatography and depleted of monocytes by treatment with a monoclonal anti-human ia-like (dr lo ... | 1982 | 6984019 |
| characterization of tufted streptococci isolated from the "corn cob" configuration of human dental plaque. | streptococci isolated from "corn cob" configurations of human dental plaque possess a polar fibrillar tuft extending 100 to 150 nm from one pole of the cell. the two strains studied were physiologically related to the streptococcus sanguis-streptococcus mitior group and were most similar to streptococcus mitis atcc 903. the corn cob streptococci were serologically related to s. sanguis serotype 1. the polar tuft contained at least two antigenically distinct components, one serologically related ... | 1980 | 6987171 |
| interactions of competent streptococcus sanguis (wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids. | competent cell-deoxyribonucleic acid (dna) interactions were examined using tritium-labeled homologous or heterologous native or denatured dnas and competent streptococcus sanguis wicky cells (strain we4). the dnas used were extracted from we4 cells, escherichia coli b cells, and e. coli bacteriophages t2, t4, t6, and t7. the reactions examined were: (i) total dna binding, (ii) deoxyribonuclease-resistant dna binding, and (iii) the production of acid-soluble products from the dna. optimal temper ... | 1980 | 6991480 |
| identification of tetracycline-resistant r-plasmids in streptococcus agalactiae (group b). | in this report, 30 tetracycline-resistant clinical isolates of group b streptococcus were examined to assess the extent to which tetracycline resistance is plasmid mediated. of these, 27 showed no physical or genetic evidence of plasmid-mediated resistance; however, one conjugative and two small (3.5 x 10(6)-dalton) multicopy non-self-transmissible tetracycline resistance plasmids were identified. the conjugative plasmid was transmissible to streptococcus faecalis as well as to streptococcus aga ... | 1980 | 7004347 |
| aphthous ulcers--a review of the literature on etiology, pathogenesis, diagnosis, and treatment. | recurrent aphthous ulcerations are seen in everyday dental practice. these lesions may easily be misdiagnosed and mistreated. a comprehensive but nondefinitive review of the literature is presented in hope that the practitioner can better diagnose, evaluate, research, and treat ras. although many modalities of treatment have been advocated, a truly effective therapeutic regimen to prevent ras does not yet seem available. additional research on the ideology, pathogenesis, and treatment of ras is, ... | 1980 | 7005287 |
| quantitative in vitro assay for "corncob" formation. | the interaction of bacterionema matruchotii with strains of streptococcus sanguis produces a structure which morphologically resembles a corncob. to determine the specific bacterial surface receptors involved in the interaction, we developed a quantitative assay. the assay consisted of mixing saline suspensions of [ch(3)-(3)h]thymidine-labeled streptococci and b. matruchotii, incubating at 37 degrees c for 2 h, and filtering the mixture through a 5-mum polycarbonate membrane filter. the free coc ... | 1980 | 7011981 |
| effect of human saliva on glucose uptake by streptococcus mutans and other oral microorganisms. | we examined the effects of human whole salivary supernatant and parotid fluid on glucose uptake by streptococcus mutans, streptococcus sanguis, streptococcus mitis, actinomyces viscosus, staphylococcus aureus, and escherichia coli. the following three effects of saliva were observed: (i) inhibition of glucose uptake (s. mutans, s. sanguis), (ii) promotion of a transient, rapid (0 to 30 s) burst of glucose uptake (s. mutans, s. sanguis), and (iii) enhancement of glucose uptake (s. mitis, a. visco ... | 1981 | 7012014 |
| ecology and nature of immunoglobulin a1 protease-producing streptococci in the human oral cavity and pharynx. | the identity and proportional distribution of immunoglobulin a1 (iga1) protease-producing streptococci in the oral and pharyngeal microflora were studied. a collection of 459 streptococcal strains, including reference strains of streptococcus species, and fresh isolates from human dental plaque and buccal and pharyngeal mucosa were identified by biochemical means and were examined for iga1 protease production. iga1 protease production was demonstrated in some, but not all, strains of streptococc ... | 1981 | 7014463 |
| interference of secretory immunoglobulin a with sorption of oral bacteria to hydroxyapatite. | the potential of secretory immunoglobulin a (s-iga) to interfere with the initial phase of dental plaque formation was studied by using an in vitro method which permits the quantitative determination of the sorption of radiolabeled oral bacterial cells to hydroxyapatite (ha) beads. the importance of specific s-iga antibodies was evaluated by a comparison of the effect of pure preparations of colostral s-iga, polymeric myeloma iga, or preabsorbed s-iga. specific antibody molecules bound at the ha ... | 1981 | 7014466 |
| bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. | recent studies have indicated that the attachment of bacteria to mucosal surfaces is the initial event in the pathogenesis of most infectious diseases due to bacteria in animals and humans. an understanding of the mechanisms of attachment and a definition of the adhesive molecules on the surfaces of bacteria (adhesins) as well as those on host cell membranes (receptors) have suggested new approaches to the prevention of serious bacterial infections: (1) application of purified adhesion or recept ... | 1981 | 7014727 |
| new method that uses binding of immunoglobulin a to group a streptococcal immunoglobulin a fc receptors for demonstration of microbial immunoglobulin a protease activity. | a new method is described for the detection of bacterial immunoglobulin a (iga) protease which splits iga into fab and fc fragments. the method takes advantage of a recent finding that receptors for iga fragments occur commonly among type 4 group a streptococci. the bacterial preparation to be tested for protease activity was first incubated with radiolabeled purified iga1 myeloma protein, and the proportion of radioactivity bound to a standard suspension of the streptococci was then measured. s ... | 1981 | 7016917 |
| genetic study of plasmid-associated zonal resistance to lincomycin in streptococcus pyogenes. | the phenomenon of zonal resistance to lincomycin, which is characteristic of most clinical isolates with lincomycin resistance in streptococcus pyogenes, has been studied. these strains grow within a defined concentration range of lincomycin (approximately 60 to 200 microgram/ml), or at lincomycin concentrations below the minimal inhibitory concentration for susceptible strains. it is shown that the zonal growth phenomenon is a stable phenotype and results from induction of resistance only withi ... | 1981 | 7018388 |
| lactoperoxidase and thiocyanate protect bacteria from hydrogen peroxide. | lactoperoxidase and thiocyanate were shown to protect escherichia coli and three oral streptococcal species from the bactericidal effect of hydrogen peroxide under aerobic conditions. lactoperoxidase in the absence of thiocyanate was also protective for two of the bacterial species in a dilution medium but potentiated hydrogen peroxide toxicity for the other two under the same conditions. the products of the reaction between hydrogen peroxide and thiocyanate in the presence of lactoperoxidase we ... | 1982 | 7033135 |
| ultrastructural location of streptococcus mutans and streptococcus sanguis antigens in carious human dentine. | the ultrastructural localization of bacterial antigens of streptococcus sanguis and of the various serotypes a,b,c,d,e,f and g of streptococcus mutans was studied in human carious dentine using the indirect peroxidase-antiperoxidase method with appropriate controls. no positive staining was seen in adjacent normal dentine. in the inner dentine underlying the cytoplasm of fibroblasts and schwann cells of unmyelinated nerve fibrils. in sclerosed tubules, or on the plasmalemma of the odontoblast pr ... | 1981 | 7035442 |
| expression of streptococcal plasmid-determined resistance to erythromycin and lincomycin in escherichia coli. | 1981 | 7035838 | |
| microbial surface interactions: reduction of the haemagglutination activity of the oral bacterium fusobacterium nucleatum by absorption with streptococcus and bacteroides. | oral strains of fusobacterium nucleatum showed haemagglutination (ha) of sheep red blood cells and attachment of ha-active f. nucleatum fragments to other microorganisms allowed a means of studying microbial surface interactions. ha-active sonicated fragments (sf) prepared from f. nucleatum were mixed with whole cell suspensions of 48 bacterial strains and, after incubation, the whole cells were separated from the non-absorbed fragments by differential centrifugation. attachment of f. nucleatum ... | 1981 | 7041865 |
| streptococcus sanguis biotype ii meningitis in a premature infant. | 1982 | 7044645 | |
| phagocytic and killing activity of human blood, gingival crevicular, and salivary polymorphonuclear leukocytes for oral streptococci. | the phagocytosis and killing of oral streptococci by blood, crevicular, and salivary polymorphonuclear leukocytes (pmnl) were examined using a visual assay based on differential staining of viable and non-viable microorganisms by acridine orange. crevicular pmnl were 83% viable, 19% contained bacteria on collection, and phagocytosis occurred in vitro in 66% of glass-adherent leukocytes. salivary pmnl were 56% viable, 11% contained bacteria on collection, and 44% phagocytosed streptococci in vitr ... | 1982 | 7045181 |
| the streptococcal flora of the tongue of the monkey macaca fascicularis. | the major streptococcal biotypes in the 8 monkeys investigated resembled streptococcus sanguis and streptococcus mitior with streptococcus salivarius forming only 5 to 6 per cent of the total colony count and being isolated from only 4 monkeys. the predominant tongue streptococci were not isolated from the dental plaque. similarly, streptococcus mutans, present in large numbers in the plaque of monkeys fed sucrose-rich diets, was isolated from the tongue of only 2 animals and formed only 2.4 per ... | 1982 | 7046706 |
| effect of altitude exposure on induction of streptococcal endocarditis in young and middle-aged rats. | young (age 2 months) and middle-aged (age 10 month) rats were injected once with a culture of streptococcus sanguis and exposed for 24 h to 7620 m altitude. at 6 d 54% of the exposed and 30% of the unexposed middle-aged rats had bacterial endocarditis. myocarditis developed in 63% of the injected exposed rats of both ages, in 11% of the injected unexposed middle-aged rats, and in none of the unexposed young adults. interstitial nephritis was found in 46-66% of the injected, unexposed young and m ... | 1982 | 7055489 |
| an effect of communal caging on the streptococcal flora of the dental plaque of monkeys (macaca fascicularis). | 22 monkeys were randomly allocated to one or other of 2 communes and fed a caries-promoting high sucrose diet. after 28 weeks commune 1 (13 monkeys) was found to harbour streptococcus mutans serotype e as the predominant streptococcal species, but no monkey in commune 2 harboured this serotype. instead the 9 monkeys of commune 2 harboured streptococcus milleri in their dental plaque. the significance of these observations on the use of monkeys as a model of human dental caries is discussed. | 1982 | 7062714 |
| clinical significance of viridans streptococci isolated from blood cultures. | the clinical significance of viridans streptococci isolated from the blood cultures of 86 patients was determined. isolates that were significant or suggestive of infection represented only 21% of the cases. among 54 isolates for which the species was known, streptococcus sanguis ii was the most common. however, a significant association between species and clinical significance was not found. | 1982 | 7068840 |
| septic sternoclavicular arthritis with pasteurella multocida and streptococcus sanguis. | 1982 | 7073777 | |
| adsorption of lysozyme from human whole saliva by streptococcus sanguis 903 and other oral microorganisms. | several strains of streptococcus sanguis, streptococcus mutans, streptococcus mitis, actinomyces viscosus, and actinomyces naeslundii plus fresh isolates of streptococcus salivarius were surveyed for their abilities to deplete lysozyme from human-whole-saliva supernatant. bacteria were incubated in saliva for 60 min at 37 degrees c and then removed by centrifugation, and the recovered supernatant solutions were assayed for lysozyme activity by using whole cells of micrococcus lysodeikticus as th ... | 1982 | 7076291 |
| role of granulocytes in experimental streptococcus sanguis endocarditis. | we investigated the role of granulocytes during the induction and course of experimental streptococcus sanguis endocarditis in rabbits by depleting blood granulocytes with nitrogen mustard. the induction of the endocarditis was not influenced by granulocytopenia: the 50% infectious dose was 5.4 x 10(4) colony-forming units in normal and granulocytopenic rabbits. however, granulocytopenia influenced the curse of the endocarditis, as shown by a significant increase in the number of colony-forming ... | 1982 | 7076302 |
| inhibitors of coaggregation between actinomyces viscosus t14v and streptococcus sanguis 34: beta-galactosides, related sugars, and anionic amphipathic compounds. | coaggregation between actinomyces viscosus t14v (t14v) and streptococcus sanguis 34 (ss34) depends upon specific reaction between lectin on t14v and carbohydrate on ss34. studies on coaggregation inhibition by sugars related to d-galactose, beta-galactosides, and amphipathic molecules revealed: (i) d-fucose, d-talose approximately equal to d-galactose, which was 0.2 potency of lactose. no other hexoses or pentoses inhibited at 0.1 m. (ii) gal beta (1 leads to 3)galnac alpha och2c6h5 was the most ... | 1982 | 7076303 |
| influence of sodium and potassium ions on acid production by washed cells of streptococcus mutans ingbritt and streptococcus sanguis nctc 7865 grown in a chemostat. | a comparison was made of acid production by cells of streptococcus mutans ingbritt and s. sanguis nctc 7865 that had been washed twice and incubated in different concentrations of sodium and potassium ions. organisms were grown under defined conditions in a chemostat under both glucose limitation and glucose excess conditions at a dilution rate of 0.1 h(-1) (mean generation time, 6.9 h). acid production after a pulse of glucose, sucrose, and fructose was measured by ph fall experiments and as a ... | 1982 | 7085068 |
| production of lipoteichoic acid by lactobacilli and streptococci grown in different environments. | representative strains of streptococcus sanguis serotype 2 and of four lactobacillus species were examined for the production of cellular and extracellular lipoteichoic acid (lta) when grown at ph 6.0 in batch culture to the stationary phase with either glucose or fructose. extracellular lta was a minor component in all cases except for l. fermentum and l. casei nird r094 grown in fructose. the total amount of lta (cellular and extracellular) produced by fructose-grown cultures was also consider ... | 1982 | 7095852 |
| specificity of salivary-bacterial interactions: ii. evidence for a lectin on streptococcus sanguis with specificity for a neuac alpha 2, 3ga1 beta 1, 3ga1nac sequence. | 1982 | 7104000 | |
| isolation and analysis of sacculi from streptococcus sanguis. | sacculi were prepared from streptococcus sanguis 34 by exhaustive extraction of bacteria with hot 1% sodium dodecyl sulfate-0.5% 2-mercaptoethanol. lyophilized residue was dissociated by brief sonication to single bodies closely resembling streptococci in phase-contrast microscopic density, staining properties, and morphology. electron micrographs revealed bodies that contained variable amounts of cellular contents and were bounded by intact cell walls. chemical analyses of sacculi demonstrated ... | 1982 | 7107558 |
| growth inhibition of oral streptococci in saliva by anionic proteins from two caries-free individuals. | mixed saliva from two caries-free individuals possessed antimicrobial activity toward streptococcus mutans and s. sanguis. this inhibitory activity was attributed to the presence of a group of four anionic proteins each of which strongly inhibited the growth of the oral streptococci in a saliva protein-based medium but not in a medium containing amino acids as a nitrogen source. these proteins, with isoelectric points of 4.70, 4.90, 4.98, and 5.05, respectively, neither reacted with antisera to ... | 1982 | 7118248 |
| failure of erythromycin in preventing bacterial endocarditis. | use of oral erythromycin to prevent bacterial endocarditis has been accepted in the treatment of penicillin-allergic patients who required oral or sinusal surgery. a case of streptococcus sanguis endocarditis, however, developed in a patient following intravenous erythromycin administration during surgery on the maxillary sinus. the organism isolated proved resistant to erythromycin. this isolation of an erythromycin-resistant organism, together with previous reports of the drug's failure in pre ... | 1982 | 7125782 |
| isolation and characterization of coaggregation-defective mutants of actinomyces viscosus, actinomyces naeslundii, and streptococcus sanguis. | spontaneously occurring coaggregation-defective (cog-) mutants of oral actinomycetes and streptococci were isolated and used to study interactions between cells of these two kinds of bacteria. cog- mutants of each kind of bacteria were isolated by a simple enrichment scheme. parent strains were mixed with a coaggregating partner strain, coaggregated cells were removed by low-speed centrifugation, and non-coaggregated cells were recycled by the addition of more partner strain cells. cog- mutants ... | 1982 | 7129635 |
| biosynthesis of glucosyl monophosphoryl undecaprenol and its role in lipoteichoic acid biosynthesis. | a glucophospholipid was detected in an incubation mixture containing udp-glucose, mgcl2, atp, and a particulate enzyme prepared from streptococcus sanguis. the synthesis of this lipid was inhibited strongly by udp and moderately by ump. the molar ratio of glucose to phosphate in the purified lipid was found to be 1:1. glucose and glucose 1-phosphate were released by mild alkaline hydrolysis of the glucophospholipid. the lipid produced by mild acid degradation of the purified lipid yielded a thin ... | 1982 | 7130126 |
| successful prophylaxis against experimental streptococcal endocarditis with bacteriostatic antibiotics. | because bacteriostatic concentrations of vancomycin are effective in prophylaxis against endocarditis due to streptococcus sanguis in rats, the efficacy of three other bacteriostatic antibiotics was investigated against three different streptococcal species that cause subacute endocarditis in humans: streptococcus intermedius. s. sanguis, and streptococcus mitior. rats were challenged by intravenous injection of 2 x 10(5) colony-forming units of streptococci 24 hr after intracardiac insertion of ... | 1982 | 7142750 |
| enhanced saliva-mediated bacterial aggregation and decreased bacterial adhesion in caries-resistant versus caries-susceptible individuals. | a study of saliva-mediated aggregation and adhesion has been carried out in a group of caries-resistant (cr) and caries-susceptible (cs) individuals. the submandibular saliva of the cs group had a much greater potency, as determined by dilution, in promoting adherence to hydroxyapatite beads than did the saliva of cr group. in contrast, the cr group demonstrated a twofold enhancement of saliva-mediated aggregation compared with the cs group. these observations support the hypothesis that saliva- ... | 1982 | 7152662 |
| attachment of oral cytophaga species to hydroxyapatite-containing surfaces. | model systems simulating the cementum portion of teeth were used to characterize the attachment process by which certain species of oral cytophaga initiate the colonization of the tooth root surface in vitro. the adsorption of these bacteria to spheroidal hydroxyapatite beads and mechanically powdered root material followed langmuir isotherm kinetics. from such data, the number of binding sites per 20 mg of substrate and the affinity constants were evaluated for two strains of cytophaga sp. rest ... | 1980 | 7216436 |
| transformation of streptococcus sanguis with monomeric pva736 plasmid deoxyribonucleic acid. | monomeric and oligomeric forms of a 5.0 x 10(6)-dalton plasmid (conferring erythromycin resistance) were able to genetically transform naturally competent streptococcus sanguis. transformation with electrophoretically purified monomer was a second-order process, whereas transformation with a dye-buoyant density gradient-purified plasmid preparation followed one-hit kinetics. | 1981 | 7217019 |
| constrictive pericarditis due to streptococcus sanguis. | we have described the third case of pericarditis caused by anaerobic streptococci in children. despite operative drainage and high-dose penicillin therapy, there was rapid progression to constrictive pericarditis. complete pericardiectomy was life-saving. | 1981 | 7221646 |
| bacterial aggregating activity in human saliva: comparisons of bacterial species and strains. | using a quantitative assay to measure saliva-mediated bacterial aggregating activity, we have surveyed 20 streptococcal strains with saliva samples obtained from a large population study. individual saliva samples demonstrated characteristic levels of aggregating activity for streptococcus sanguis m5. in general, high activity for this strain was associated with high activity for other strains of s. sanguis. streptococcus mitis, and streptococcus salivarius. the population distribution of aggreg ... | 1981 | 7228395 |
| effect of bacterial aggregation on the adherence of oral streptococci to hydroxyapatite. | several in vitro assay systems to measure the adherence of human dental plaque bacteria to solid surfaces such as teeth, glass, and hydroxyapatite have been published. in many studies a variety of macromolecular solutes have been used to study the adherence process. often these solutes are able to aggregate the test bacterial and thus may alter the outcome of adherence experiments. in this study, the effects of the aggregation of streptococcus sanguis on adherence to spheroidal hydroxyapatite is ... | 1981 | 7228408 |
| isolation and immunobiological classification of streptococcus sanguis from human tooth surfaces. | a total of 113 pure cultures of streptococcus sanguis were obtained from dental plaque samples of 64 subjects. all isolates synthesized glucan from sucrose, elaborated peroxide, and were alpha-hemolytic. two biotypes and four serotypes were differentiated within the species. biotype a (95 isolates) fermented salicin and inulin and hydrolyzed arginine and esculin, whereas biotype b (18 isolates) did not possess these activities. the isolates were serotyped with autoclaved extracts against whole-c ... | 1980 | 7229009 |
| the deposition of streptococcus sanguis nctc 7868 from a flowing suspension. | measurements were made of the rates at which continuously cultured streptococcus sanguis nctc 7868 cells accumulated on the inside surfaces of narrow glass capillaries from suspensions of the bacteria flowing down the capillaries at different velocities. initially, the rate of accumulation of bacteria on the clean walls of the capillary was rapid. the deposition rate decreased with time, however, resulting in a saturation coverage of the glass surface which was considerably less than a monolayer ... | 1980 | 7229614 |
| pulmonary clearance and phagocytic cell response to normal pharyngeal flora. | because human lungs are repetitively inoculated with the normal bacterial flora of the pharynx, we determined the pulmonary clearance of representative species after aerosol inoculation of a murine model, and characterized the phagocytic cell response by bronchoalveolar lavage. viable bacteria remaining in the lungs at 1, 2, and 4 h were: streptococcus sanguis, 24%, 8%, and 1%; streptococcus salivarius, 49%, 24%, and 5%; neisseria catarrhalis, 69%, 49%, and 22%. clearance of streptococcus sangui ... | 1981 | 7235361 |
| manganese antagonizes the inhibitory effect of fluoride on the glucose metabolism of streptococcus mutans. | manganese stimulated the utilization of glucose by streptococcus mutans, streptococcus sanguis, streptococcus mitior and streptococcus milleri. s. mutans serotype c strains formed larger amounts of intracellular polysaccharide, lactic acid and produced a lower terminal ph in glucose broth when grown in the presence of 0.5 mm mn. manganese stimulated the utilization of glucose by resting cell suspensions of each of the four oral streptococcal species examined. fluoride inhibited the utilization o ... | 1980 | 7242378 |
| adherence of streptococcus mutans and streptococcus sanguis to salivary components bound to glass. | adherence of radiolabeled streptococcus mutans and streptococcus sanguis to saliva-treated glass surfaces was studied under conditions which minimized bacteria-glass interactions. treatment of glass with an alkylsilane solution decreased nonspecific bacterial adherence and enhanced adsorption of radiolabeled salivary components to these surfaces. addition of triton x-100 to the bacterial suspensions also reduced nonspecific adherence to siliconized glass, but did not affect adherence to salivary ... | 1981 | 7251139 |
| genetic transformation of streptococcus mutans. | three strains of streptococcus mutans belonging to serotypes a, c, and f were transformed to streptomycin resistance by deoxyribonucleic acids derived from homologous and heterologous streptomycin-resistant strains of s. mutans and streptococcus sanguis strain challis. homologous transformation of s. mutans was less efficient than heterologous transformation by deoxyribonucleic acids from other strains of s. mutans. | 1981 | 7251168 |
| role of bacteriocin during plaque formation by streptococcus salivarius and streptococcus sanguis on a tooth in an artificial mouth. | 1981 | 7251515 | |
| genetic transformation in streptococcus sanguis. competence factor and competence factor inactivator. | genetic transformation in streptococcus sanguis is a complex, multi-step process, involving several factors. the competence factor (cf), occurring in the culture filtrates of some strains, is essential in streptococcal transformation, but other factors are also involved. the presence of serum during growth is not obligatory for the preparation of active culture filtrate, but serum increases the amount and duration of cf activity of some strains. the stability of the cf activity differs distinctl ... | 1981 | 7257776 |
| genetic transformation of streptococcus sanguis. further studies on the production and isolation of the competence factor. | two new media were developed, containing only the dialyzable components of todd hewitt broth (th) with (medium ii) or without (medium iv) inactivated horse serum. the two media were used to detect activity of the competence factor (cf) and the competence factor inactivator (cgi) of streptococcus sanguis, and in preliminary experiments of cf isolation. because all the dissolved substances with molecular weight (mol. wt.) less than 12,000 can be removed from these media by dialysis, leaving the cf ... | 1981 | 7257777 |
| lactose-reversible coaggregation between oral actinomycetes and streptococcus sanguis. | freshly isolated strains of oral actinomycetes were obtained from human dental plaque and were tested for the ability to coaggregate with common laboratory stock strains of streptococcus sanguis. strains belonging to the genera actinomyces, arachnia, bifidobacterium, and bacterionema were isolated. only members of the genus actinomyces coaggregated with the streptococci, and only actinomyces viscosus and actinomyces naeslundii exhibited lactose-reversible interactions. a total of 61 strains, con ... | 1981 | 7263074 |
| experimental endocarditis caused by streptococcus sanguis: single and combined antibiotic therapy. | the effectiveness of penicillin g, fosfomycin, and cefoxitin alone and in combination was studied in vitro and in the treatment of left-sided streptococcus sanguis endocarditis in rabbits. in vitro, the combinations penicillin g plus fosfomycin, penicillin g plus cefoxitin, and fosfomycin plus cefoxitin were synergistic or partially synergistic for s sanguis. therapy with the combinations was more effective in eradicating the species from cardiac vegetations that was that with each antibiotic us ... | 1981 | 7283409 |
| salivary immunoglobulin a antibodies reacting with antigens from oral streptococci: longitudinal study in humans. | the salivary immunoglobulin a (iga) activity to antigens from four common oral streptococci was analyzed in samples from five humans. from each individual, parotid and whole saliva were collected 12 times over a period of 4 months. in samples collected at different occasions, the salivary iga activity varied considerably. the variations showed a covariation with the concentration of total iga in the saliva samples. a covariation was also found between salivary iga antibodies of different specifi ... | 1981 | 7287178 |
| vancomycin prophylaxis of experimental streptococcus sanguis. inhibition of bacterial adherence rather than bacterial killing. | using a strain of streptococcus sanguis tolerant to vancomycin to infect aortic vegetations in rats, we found that prophylactic intravenous vancomycin given 30 min before bacterial challenge decreased the incidence of endocarditis from 88 to 8% (p less than 10(-5)). because peak vancomycin serum levels were below the minimal bactericidal concentration, mechanisms of protection other than bacterial killing were investigated. s. sanguis were incubated with inhibitory concentration of vancomycin (5 ... | 1981 | 7287904 |
| sucrose-induced ecological response of experimental dental plaques from caries-free and caries-susceptible human volunteers. | microbial succession, experimental cariogenicity, and sucrose metabolism were examined in dental plaques which developed on sterile bovine enamel inserts in acrylic palatal appliances. the appliances were worn for a period of 14 days by 10 caries-free and 10 caries-susceptible human volunteers. three of six enamel inserts on each appliance were exposed extraorally to 10% sucrose in 0.85% saline six times a day, and three were exposed simultaneously to 0.85% saline as a control environment. the r ... | 1981 | 7333665 |
| enolases from fluoride-sensitive and fluoride-resistant streptococci. | the enolase from a highly fluoride-sensitive strain of streptococcus salivarius and its fluoride-resistant mutant, as well as those from strains of streptococcus sanguis and streptococcus mutans with intermediate and low sensitivities to fluoride have been shown to be inhibited by fluoride. comparisons of the purified, strain-specific enzymes showed a high degree of similarity for all preparations. the michaelis constants for the substrate 2-phosphoglycerate were 1.3 x 10(-4) to 2.4 x 10(-4) m, ... | 1981 | 7333671 |
| viability of six species of normal oropharyngeal bacteria after exposure to cigarette smoke in vitro. | bacteria in culture medium at 37 degrees c were subjected to eight puffs of smoke from one cigarette, and viable cell counts were done at intervals ranging from 0 min to 3 h after exposure to smoke. the cigarette contained 23.0 mg of tar and 1.4 mg of nicotine. each 25.0 ml puff of smoke from a mechanical smoking apparatus was passed over a 1.0 ml suspension of bacteria dispersed over a 25 cm2 surface in a sterile flask. filtered air was in contact with the suspension between puffs, and during t ... | 1981 | 7339446 |
| presynaptic donor dna-protein complexes in transformation of streptococcus sanguis: identification of the protein component. | 1980 | 7378079 | |
| effects of monocytopenia and anticoagulation in experimental streptococcus sanguis endocarditis. | the role of blood monocytes in the attachment of streptococci to endocardial vegetations was investigated in an experimental streptococcus sanguis endocarditis by depletion of blood monocytes with the cytostatic drug vp 16-213 alone and combined with anticoagulant treatment with warfarin sodium. the numbers of streptococci in the vegetations of control, monocytopenic, and monocytopenic/anticoagulated rabbits were comparable. in the vegetations streptococci were found mainly in areas free of phag ... | 1980 | 7378272 |
| simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci. | a simple procedure for rapidly demonstrating small plasmids (less than 10 megadaltons) in oral streptococci is described. logarithmic-phase, glycine-treated cells from 1.5-ml broth cultures were converted to osmotically fragile forms and lysed with sodium dodecyl sulfate. after the hydrodynamic shearing of host chromosomal deoxyribonucleic acid, such lysates were analyzed by low-voltage agarose gel electrophoresis. small plasmids, migrating significantly faster than chromosomal deoxyribonucleic ... | 1980 | 7396483 |
| genetic transformation of streptococcus sanguis (challis) with cryptic plasmids from streptococcus ferus. | by using the basic methodology initially published by kretschmer et al. (j. bacteriol. 124:225-231, 1975), we have been able to introduce phenotypically cryptic plasmids from streptococcus ferus (formerly streptococcus mutans subsp. ferus) into streptococcus sanguis by genetic transformation. in this system, the entry of the cryptic plasmids is selected indirectly. this is effected with transforming deoxyribonucleic acid mixtures in which the cryptic plasmid deoxyribonucleic acid is present in a ... | 1980 | 7399689 |
| modulation of immunoreactivity to periodontal disease-associated microorganisms during pregnancy. | the lymphocyte blastogenic response to a panel of antigens and mitogens was assessed in a group of 20 women throughout their pregnancy. in addition, a group of five nonpregnant women was monitored simultaneously to identify variations in response to the same stimulants. the stimulants included orally associated bacterial antigens (streptococcus sanguis, actinomyces viscosus, bacteroides asaccharolyticus, bacteroides melaninogenicus subsp. intermedius, bacteroides [capnocytophaga] ochraceus, and ... | 1980 | 7399691 |
| lymphoproliferative responses to oral bacteria in humans with varying severities of periodontal disease. | we assessed in vitro the lymphocyte blastogenic resp onsess of peripheral blood lymphocytes to antigen extracts of a large battery of oral microorganisms in a population of humans with varying severities of periodontal disease. when the magnitudes and frequencies of statistically positive blastogenic responses to various oral microorganisms were compared, three general patterns emerged. the actinomyces species proved to be potent stimulators of lymphocyte blastogenesis in most subjects tested, w ... | 1980 | 7399694 |
| microflora and chemical composition of dental plaque from subjects with hereditary fructose intolerance. | we compared the microbiological and chemical composition of dental plaque from subjects with hereditary fructose intolerance who restrict their dietary sugar intake with that of control subjects who do not. the two groups showed no significant differences in chemical composition of plaque: the mean protein, carbohydrate, calcium, magnesium, and phosphate contents were similar. dental plaque from both groups contained similar numbers of total colony-forming units per microgram of plaque protein, ... | 1980 | 7399699 |
| streptococcus sanguis sepsis and meningitis: a complication of vacuum extraction. | 1980 | 7416226 | |
| experimental streptococcus sanguis endocarditis: immune complexes and renal involvement. | an increase of agglutinins and circulating immune complexes (cic) was observed in the sera of rabbits after the induction of bacterial endocarditis (be) by intravenous injection of live streptococci into animals with a non-bacterial thrombotic endocarditis. rabbits immunized for 12 days with heat-killed streptococci developed higher levels of agglutinins and cic than did those unimmunized during be. the levels remained elevated in the immunized rabbits after the induction of streptococcal endoca ... | 1980 | 7418263 |
| bactericidal effect of hydrogen peroxide is prevented by the lactoperoxidase-thiocyanate system under anaerobic conditions. | streptococcus sanguis and peptostreptococcus anaerobius were exposed to various combinations of the components of the lactoperoxidase-thiocyanate-hydrogen peroxide system. the bactericidal effect of hydrogen peroxide was prevented under anaerobic conditions by lactoperoxidase together with thiocyanate, but not by lactoperoxidase or thiocyanate alone. thiocyanate was effective already at a molar ratio to hydrogen peroxide of 1:100. | 1980 | 7429633 |
| the effects of fluoride on the growth of oral streptococci. | four species of oral streptococci were examined for their ability to grow in a complex medium supplemented with naf, and at various ph levels. streptococcus mutans strains were the most susceptible to inhibition, with streptococcus mitior and streptococcus sanguis being the least affected. the s. mutans genotype comprising serotypes c, e and f was more susceptible to inhibition by fluoride than was the genotype comprising serotypes d and g. the results suggest that the fluoride concentration of ... | 1980 | 7432189 |
| an immunoelectrophoretic analysis of the strep. sanguis and adult human oral mucosa antigen extracts used for immunological investigations of recurrent aphthous stomatitis. | water-soluble antigens of strep. sanguis strain 2a, atcc 10556, and adult human oral mucosa (ahom) were analysed by four immunoelectrophoretic methods. crossed immunoelectrophoresis (cie), using rabbit antibodies raised against the soluble antigens, revealed 26 antigens in the standard strep. sanguis antigen extract and 16 antigens in the standard adult human oral mucosa extract. all 26 strep. 2a antigen-antibody precipitates observed in the cie were identified as streptococcal components. eleve ... | 1980 | 7446131 |
| infective endocarditis, 1970-1979. a study of culture-positive cases in st. thomas' hospital. | ninety-three episodes of culture-positive endocarditis occurred in 88 patients in the years 1970 to 1979. streptococci caused two-thirds of the cases, with streptococcus mitior and streptococcus sanguis most common. among the group d streptococci, streptococcus bovis was much more common than streptococcus faecalis. staphylococci accounted for a quarter of the episodes. streptococcal endocarditis was commonest in the elderly, whereas staphylococcal endocarditis occurred at all ages. degenerative ... | 1980 | 7465764 |
| kinetic analysis of microbial adhesion. | 1995 | 7476386 | |
| bacterial adhesion to hydroxylapatite. | 1995 | 7476417 | |
| isolation and structural characterization of adhesin polysaccharide receptors. | the procedure for the purification of the adhesin polysaccharide receptor and its hexasaccharide repeating unit from whole s. oralis atcc 55229 by chemical, enzymatic, and chromatographic techniques has been described. chemical, chromatographic, and mass spectrometric procedures allow preliminary structural characterization of the hexasaccharide repeating unit and polysaccharide. the structural characterizations of the hexasaccharide and polysaccharide are completed using several 1d and 2d nmr t ... | 1995 | 7476419 |
| characteristics of accumulation of oral gram-positive bacteria on mucin-conditioned glass surfaces in a model system. | strains of streptococcus, actinomyces and lactobacillus were grown on glass surfaces in semi-defined medium (ph 7.0) with mucin, at a dilution rate of d = 0.1 h-1, in a modified chemostat. the accumulation of cells followed four phases. in phase 1 (0-1 h), cells did not divide on the surfaces and adhesion accounted for rapid accumulation. phase 2 (1-4 h) comprised adhesion and cell division, and accumulation slowed, cell number doubling times (cdt) streptococcus, 2.7 h to 8.6 h, actinomyces, 2.3 ... | 1994 | 7478748 |
| growth-inhibitory effect of pyrophosphate on oral bacteria. | the purpose of this investigation was to determine whether pyrophosphate, the anticalculus component of tartar-control dentifrices, exerts antimicrobial activity against oral bacteria commonly found in supragingival plaque. minimal inhibitory concentrations of pyrophosphate were determined for streptococcus sanguis, streptococcus mutans (serotype c), actinomyces viscosus and actinomyces naeslundii. all of the bacteria tested were susceptible to pyrophosphate with identical minimal inhibitory con ... | 1994 | 7478751 |
| in vitro modulation of oral bacterial adhesion to saliva-coated hydroxyapatite beads by milk casein derivatives. | bovine caseinate, derivatives of its glycosylated moiety [caseinoglycomacropeptide (cgp)], and caseinophosphopeptides were evaluated as inhibitors of adhesion of oral bacteria to saliva-coated hydroxyapatite beads (s-ha). all milk casein-derived components behaved as potent inhibitors of streptococcus sanguis omz 9 and streptococcus sobrinus omz 176 adhesion to s-ha, whereas neither bovine serum albumin nor polyethyleneglycol were able to interfere with the adhesion of these strains. by contrast ... | 1994 | 7478758 |
| biochemical change exhibited by oral streptococci resulting from laboratory subculturing. | the intent of this study was to assess the effects of continued laboratory subculturing on selected biochemical properties of oral streptococci freshly isolated from dental plaque. six fresh isolates (3 streptococcus mutans and 3 non-mutans) and 2 laboratory strains were subcultured daily for a total of 225 transfers, and cells were harvested every 75 transfers from duplicate batch cultures grown with glucose at a constant ph. eleven biochemical properties were assayed with cells, membranes and ... | 1994 | 7478760 |
| immunization of mice by oral colonization with live recombinant commensal streptococci. | to test the use of recombinant streptococci as live vaccine vectors, colonization/immunization experiments were performed with streptococcus gordonii expressing heterologous cell-surface antigens. three isogenic strains of s. gordonii were used: a wild-type, a recombinant expressing the m6 protein of streptococcus pyogenes, and a recombinant expressing the e7 protein of human papillomavirus type 16 as a fusion with the m6 protein. a single dose of live bacteria was used to inoculate outbred mice ... | 1995 | 7483795 |
| hemolytic anemia in a patient with subacute bacterial endocarditis due to streptococcus sanguis. | we describe a case of hemolytic anemia with subacute bacterial endocarditis due to streptococcus sanguis. the major hematological features of the patient were a normocytic anemia with reticulocytosis, an elevation of serum lactate dehydrogenase, and a marked reduction of serum haptoglobin. all these signs of hemolytic anemia fluctuated in parallel with changes in inflammatory activity and were dramatically improved by the administration of benzylpenicillin, suggesting that the infection with s. ... | 1995 | 7484020 |
| streptococcus oralis previously identified as uncommon 'streptococcus sanguis' in behçet's disease. | the relation between the biochemical and serological properties of 'streptococcus sanguis' was studied to characterize the strains isolated from dental plaque of patients with behçet's disease and controls. seven reference and 100 clinical strains preserved by the behçet's disease research committee of japan were identified using established criteria and differentiated with antisera against strep. oralis atcc 10557, strep. sanguis atcc 10556 and 'strep. sanguis' st7, compatible with the criteria ... | 1995 | 7487566 |
| bacteria in human mouths involved in the production and utilization of hydrogen peroxide. | earlier studies have demonstrated that pure cultures of oral streptococci produce hydrogen peroxide but none has found any free peroxide in dental plaque or salivary sediment despite streptococci being major components of their mixed bacterial populations. the absence of peroxide in plaque and sediment could be due to the dominance of its destruction over its formation by bacterial constituents. to identify which of the oral bacteria might be involved in such a possibility, pure cultures of 27 d ... | 1995 | 7487577 |
| adsorption of saliva-coated and just-harvested streptococcus sanguis to saliva-coated hydroxyapatite beads. | it is possible that salivary proteins on bacterial cells have some influence on the adsorption of oral bacteria to the surface of oral tissues. the aim of this study was to clarify the influence of salivary proteins attached to the surface of streptococcus sanguis atcc 10556 (s. sanguis) on bacterial adsorption to hydroxyapatite beads treated with salivary proteins (s-ha beads). just-harvested cells (n-system) and saliva-coated cells (c-system) were used. the results showed that adsorption to s- ... | 1995 | 7490611 |
| antibacterial action of dental cements: an in vitro study. | the antibacterial activity of seven commercially available dental cements (eugespad, dentical, dycal, expaliner, pr. scell, pr. base cement, pr. lining cement) against 1) bacterial species implicated in carious lesions or in dental plaque (actinomyces israelii atcc 10048, actinomyces viscosus atcc 19246, streptococcus mutans atcc 25175, streptococcus sanguis atcc 10557) and 2) bacterial samples of stimulated saliva was studied, in vitro, using a modification of the method of mccomb and ericson ( ... | 1995 | 7492899 |
| therapeutic advantage of recombinant human plasminogen activator in endocarditis: evidence from experiments in rabbits. | in infective endocarditis vegetations are stabilized by fibrin. to learn if fibrin digestion would be therapeutic, experimental endocarditis was induced in rabbits by inoculation with a platelet-aggregating strain (agg+) of streptococcus sanguis and treated with recombinant tissue plasminogen activator (rt-pa), rt-pa with penicillin, or penicillin alone. control rabbits were inoculated with saline. all treatments of agg+ endocarditis reduced the mass of valvular vegetations and clinical signs of ... | 1995 | 7495078 |
| use of restriction fragment polymorphism analysis of rrna genes to assign species to unknown clinical isolates of oral viridans streptococci. | this study evaluated restriction fragment length polymorphisms of rrna genes (ribotyping) for genotypic identification of 53 oral isolates classified as "streptococcus sanguis" by colony morphology. isolates were from 8-h buccal plaque on lower first permanent molars of 20 subjects. dna was digested with aatii and hybridized with digoxygenin-labeled cdna of escherichia coli 16s and 23s rrna. strains were ribotyped again with alwni or pvuii on the basis of the presence or absence of a 2,290-bp aa ... | 1994 | 7512095 |
| fine antigen specificity of human gamma delta t cell lines (v gamma 9+) established by repetitive stimulation with a serotype (kth-1) of a gram-positive bacterium, streptococcus sanguis. | we have established human gamma delta t cell lines specific for streptococcus sanguis (s. sanguis) kth-1 present in normal oral cavity flora. the cd4-cd8-cd3+v gamma 9+v delta 1-cd45ro+ cd25+ t cell lines showed a proliferative response to the streptococcal antigen (ag) in the presence of autologous antigen-presenting cells without apparent evidence of hla restriction. the proliferative response of the gamma delta t cell lines was completely blocked by anti-tcr gamma delta monoclonal antibody (m ... | 1994 | 7517873 |
| the use of lectins in monitoring degradation of oligosaccharide chains in mucin by oral streptococci. | the ability of utilize mucin oligosaccharides as sources of carbohydrate and energy is believed to be an important mechanism in the ecology of oral streptococci. in this study we have used digoxigenin-labelled lectins of various specificities to monitor changes in the nonreducing end groups of oligosaccharide chains following their degradation by streptococcus oralis ny 586 and streptococcus sanguis ny 584. the reaction of degraded mucin with peanut lectin, that recognizes the core disaccharide ... | 1994 | 7520835 |
| identification of a vibrio cholerae toxr-activated gene (tagd) that is physically linked to the toxin-coregulated pilus (tcp) gene cluster. | the toxin-coregulated pilus (tcp)-encoding gene cluster (tcp) specifies a type-iv pilus that is a major colonization determinant of vibrio cholerae. we have identified a gene 200 bp upstream from the tcp cluster that requires toxr for expression. we have designated this gene tagd (toxr-activated gene) and have shown that tagd is encoded on a 600-nt transcript. the deduced tagd product is a 164-amino-acid polypeptide (20 kda). interestingly, tagd shares a high degree of similarity to a protein of ... | 1994 | 7523254 |
| determination of 16s rrna sequences of streptococcus mitis and streptococcus gordonii and phylogenetic relationships among members of the genus streptococcus. | we determined the 16s rrna sequences of the type strains of streptococcus mitis and streptococcus gordonii and calculated the phylogenetic distances between those organisms and other members of the genus streptococcus. the viridans group streptococci were separated into five phylogenetic groups; we named these groups the anginosus group, the mitis group, the salivarius group, the bovis group, and the mutans group. s. mitis and s. gordonii clustered in the mitis group together with streptococcus ... | 1995 | 7537076 |