Publications

primary structure and biochemical characterization of yeast gtpase-activating proteins with substrate preference for the transport gtpase ypt7p.	small gtpases of the ypt/rab family are regulators of vesicular protein trafficking in exo-and endocytosis. gtpase-activating proteins (gap) play an important role as down regulators of gtpases. we here report the molecular cloning of a novel gap-encoding gene (gyp7, for gap for ypt7) by high expression from a saccharomyces cerevisiae genomic library. the gyp7 gene encodes a hydrophilic protein with a molecular mass of 87 kda. comparison of its primary sequence with that of the three other known ...	1999	10091609
high-level expressing yac vector for transgenic animal bioreactors.	the position effect is one major problem in the production of transgenic animals as mammary gland bioreactors. in the present study, we introduced the human growth hormone (hgh) gene into 210-kb human alpha-lactalbumin position-independent yac vectors using homologous recombination and produced transgenic rats via microinjection of yac dna into rat embryos. the efficiency of producing transgenic rats with the yac vector dna was the same as that using plasmid constructs. all analyzed transgenic r ...	1999	10092121
pax6 and pdx1 form a functional complex on the rat somatostatin gene upstream enhancer.	the somatostatin upstream enhancer (sms-ue) is a highly complex enhancer element. the distal a-element contains overlapping pdx1 and pbx binding sites. however, a point mutation in the a-element that abolishes both pdxl and pbx binding does not impair promoter activity. in contrast, a point mutation that selectively eliminates pdx1 binding to a proximal b-element reduces the promoter activity. the b-element completely overlaps with a pax6 binding site, the c-element. a point mutation in the c-el ...	1999	10094480
identification of nsf as a beta-arrestin1-binding protein. implications for beta2-adrenergic receptor regulation.	previous studies have demonstrated that beta-arrestin1 serves to target g protein-coupled receptors for internalization via clathrin-coated pits and that its endocytic function is regulated by dephosphorylation at the plasma membrane. using the yeast two-hybrid system, we have identified a novel beta-arrestin1-binding protein, nsf (n-ethylmaleimide-sensitive fusion protein), an atpase essential for many intracellular transport reactions. we demonstrate that purified recombinant beta-arrestin1 an ...	1999	10196135
[effect of biologically active food additives containing autolysate of baker's yeast and spirulina on intestinal permeability in an experiment].	influence of bioactive food supplements (bfa) intake on intestinal barrier permeability to macromolecules of polyethylene glycol 4000 was studied in rats with intestinal anaphylaxis and after external gamma-irradiation. bfa studied included autolysed baker's yeast ("vitasil") and edible algae spirulina platensis. intake of complex additive vitasil + spirulina resulted in significant diminution of permeability before irradiation and its partial normalization (24% decrease) after irradiation. spir ...	1999	10198958
differentially expressed genes in c6.9 glioma cells during vitamin d-induced cell death program.	c6.9 rat glioma cells undergo a cell death program when exposed to 1, 25-dihydroxyvitamin d3 (1,25-d3). as a global analytical approach, we have investigated gene expression in c6.9 engaged in this cell death program using differential screening of a rat brain cdna library with probes derived from control and 1,25-d3-treated cells. using this methodology we report the isolation of 61 differentially expressed cdnas. forty-seven cdnas correspond to genes already characterized in rat cells or tissu ...	1998	10200452
the peroxin pex14p. cdna cloning by functional complementation on a chinese hamster ovary cell mutant, characterization, and functional analysis.	rat cdna encoding a 376-amino acid peroxin was isolated by functional complementation of a peroxisome-deficient chinese hamster ovary cell mutant, zp110, of complementation group 14 (cg14). the primary sequence showed 28 and 24% amino acid identity with the yeast pex14p from hansenula polymorpha and saccharomyces cerevisiae, respectively; therefore, we termed this cdna rat pex14 (rnpex14). human and chinese hamster pex14p showed 96 and 94% identity to rat pex14p, except that both pex14p comprise ...	1999	10212238
pdk1 acquires pdk2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of prk2.	protein kinase b (pkb) is activated by phosphorylation of thr308 and of ser473. thr308 is phosphorylated by the 3-phosphoinositide-dependent protein kinase-1 (pdk1) but the identity of the kinase that phosphorylates ser473 (provisionally termed pdk2) is unknown.	1999	10226025
the mouse major histocompatibility complex: some assembly required.	we have assembled a contig of 81 yeast artificial chromosome clones that spans 8 mb and contains the entire major histocompatibility complex (mhc) from mouse strain c57bl/6 (h2b), and we are in the process of assembling an mhc contig of bacterial artificial chromosome (bac) clones from strain 129 (h2bc), which differs from c57bl/6 in the h2-q and h2-t regions. the current bac contig extends from tapasin to d17leh89 with gaps in the class ii, h2-q, and distal h2-m regions. only four bac clones we ...	1999	10319263
lipocortin v may function as a signaling protein for vascular endothelial growth factor receptor-2/flk-1.	binding of vascular endothelial growth factor (vegf) to its receptor, vegfr-2 (flk-1/kdr), induces dimerization and activation of the tyrosine kinase domain of the receptor, resulting in autophosphorylation of cytoplasmic tyrosine residues used as docking sites for signaling proteins that relay the signals for cell proliferation, migration, and permeability enhancement. we explored the vegf/receptor signaling pathway by performing a two-hybrid screen of a rat lung cdna library in yeast using the ...	1999	10329451
dietary selenium reduces the formation of aberrant crypts in rats administered 3,2'-dimethyl-4-aminobiphenyl.	human epidemiologic studies suggest that low selenium status is associated with increased cancer risk and that selenium supplementation is associated with reduction in the incidence of several cancers, including colorectal cancer. aromatic and heterocyclic amine carcinogens are thought to be important in the etiology of human colorectal cancer, but no information is available on the effects of selenium on aromatic amine-induced colon cancer. in order to investigate this effect, aberrant crypt fo ...	1999	10329505
role of acylcoa binding protein in acylcoa transport, metabolism and cell signaling.	long chain acylcoa esters (lcas) act both as substrates and intermediates in intermediary metabolism and as regulators in various intracellular functions. acylcoa binding protein (acbp) binds lcas with high affinity and is believed to play an important role in intracellular acylcoa transport and pool formation and therefore also for the function of lcas as metabolites and regulators of cellular functions [1]. the major factors controlling the free concentration of cytosol long chain acylcoa este ...	1999	10331663
t-star/etoile: a novel relative of sam68 that interacts with an rna-binding protein implicated in spermatogenesis.	rbm is an rna-binding protein encoded on the y chromosome in mammals and is expressed only in the nuclei of male germ cells. genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. of a number of potential partners for rbm identified by a yeast two-hybrid screen with testis cdna, the most frequent isolates encoded a novel rna-binding protein, termed t-star, that is closely related to sam68, an src-associated protein of unknown function. the mouse homolo ...	1999	10332027
glucuronidation of the environmental oestrogen bisphenol a by an isoform of udp-glucuronosyltransferase, ugt2b1, in the rat liver.	bisphenol a, an environmental oestrogenic chemical, was found to conjugate highly with glucuronic acid in male rat liver microsomes studied in vitro. in the various isoforms tested (1a1, 1a3, 1a5, 1a6, 1a7 and 2b1), glucuronidation of bisphenol a and of diethylstilboestrol, a synthetic crystalline compound possessing oestrogenic activity and known to be glucuronidated by liver microsomes, was catalysed by an isoform of udp-glucuronosyltransferase (ugt), namely ugt2b1, which glucuronidates some e ...	1999	10333482
signal presequences increase mitochondrial permeability and open the multiple conductance channel.	we have reported that the signal presequence of cytochrome oxidase subunit iv from neurospora crassa increases the permeability of isolated rat liver mitochondria [p. m. sokolove and k. w. kinnally (1996) arch. biochem. biophys. 336, 69] and regulates the behavior of the mutiple conductance channel (mcc) of yeast inner mitochondrial membrane [t. a. lohret and k. w. kinnally (1995) j. biol. chem. 270, 15950]. here we examine in greater detail the action of a number of mitochondrial presequences f ...	1999	10334870
amino-terminal region of secreted form of amyloid precursor protein stimulates proliferation of neural stem cells.	beta-amyloid precursor protein (app) has been reported to be expressed in the cns from the early stages of development. however, the functional role of app during early development remains unclear. in the present study, we found that the secreted form of app (sapp) significantly enhanced proliferation of neural stem cells. cells were prepared from 13-day embryonic rat neocortex, which was dissected with a pasteur pipette to make cell clusters. after 12 h of cultivation in the medium without seru ...	1999	10336659
the klpmr1 gene of kluyveromyces lactis encodes for a p-type ca(2+)-atpase.	a novel p-type ca(2+)-atpase gene has been cloned and sequenced in the yeast kluyveromyces lactis. the gene has been named klpmr1 and is localized on chromosome i. the putative gene product contains 936 residues and has a calculated molecular weight of 102,437 da. analysis of deduced amino acid sequence (klpmr1p) indicated that the encoded protein retains all the highly conserved domains characterizing the p-type atpases. klpmr1p shares 71% amino acid identity with pmr1p of s. cerevisiae, 62% wi ...	1999	10341422
heterologous expression in saccharomyces cerevisiae of an arabidopsis thaliana cdna encoding mevalonate diphosphate decarboxylase.	sequence comparison with the mevalonate diphosphate decarboxylase (mvd) amino acid sequence of saccharomyces cerevisiae identified an est clone corresponding to a cdna that may encode arabidopsis thaliana mvd (atmvd1). this enzyme catalyses the synthesis of isopentenyl diphosphate, the building block of sterol and isoprenoid biosynthesis, and uses mevalonate diphosphate as a substrate. sequencing of the full-length cdna was performed. the predicted amino acid sequence presents about 55% identity ...	1999	10344201
genetic analysis of cytochrome b5 from arachidonic acid-producing fungus, mortierella alpina 1s-4: cloning, rna editing and expression of the gene in escherichia coli, and purification and characterization of the gene product.	information on the amino acid sequences of the internal peptide fragments of cytochrome b5 from mortierella hygrophila was used to prepare synthetic oligonucleotides as primers for the polymerase chain reaction. a 100-base dna fragment was thus amplified, by using a genomic gene from mortierella alpina 1s-4 as a template, which produced polyunsaturated fatty acids such as arachidonic acid. the amplified dna fragment was used as the probe to clone both a 523-base cdna fragment and a 2.1-kilobase ...	1999	10348912
an example of intron junctional sliding in the gene families encoding squalene monooxygenase homologues in arabidopsis thaliana and brassica napus.	sequences of three arabidopsis thaliana and two brassica napus cdnas encoding squalene monooxygenase homologues (sqp1 and sqp2) are reported. southern analysis confirmed that these cdnas are derived from small gene families in both species. expression analysis indicates that sqp1 genes in b. napus are strongly expressed in leaves but not roots or developing seeds. comparison of cdna and genomic sequences indicate that the 3' splice site of an intron in these genes has undergone junctional slidin ...	1999	10350086
tandem mass spectrometry of alcohol dehydrogenase and related biomolecules.		1999	10352699
rnase treatment of yeast and mammalian cell extracts affects in vitro substrate methylation by type i protein arginine n-methyltransferases.	type i protein arginine n-methyltransferases catalyze the formation of omega-ng-monomethylarginine and asymmetric omega-ng, ng-dimethylarginine residues using s-adenosyl-l-methionine as the methyl donor. in vitro these enzymes can modify a number of soluble methyl-accepting substrates in yeast and mammalian cell extracts including several species that interact with rna. we treated normal and hypomethylated saccharomyces cerevisiae and rat1 cell extracts with rnase prior to in vitro methylation b ...	1999	10362520
x-linked dyskeratosis congenita is predominantly caused by missense mutations in the dkc1 gene.	dyskeratosis congenita is a rare inherited bone marrow-failure syndrome characterized by abnormal skin pigmentation, nail dystrophy, and mucosal leukoplakia. more than 80% of patients develop bone-marrow failure, and this is the major cause of premature death. the x-linked form of the disease (mim 305000) has been shown to be caused by mutations in the dkc1 gene. the gene encodes a 514-amino-acid protein, dyskerin, that is homologous to saccharomyces cerevisiae cbf5p and rat nap57 proteins. by a ...	1999	10364516
dominant negative murine serum response factor: alternative splicing within the activation domain inhibits transactivation of serum response factor binding targets.	primary transcripts encoding the mads box superfamily of proteins, such as mef2 in animals and zema in plants, are alternatively spliced, producing several isoformic species. we show here that murine serum response factor (srf) primary rna transcripts are alternatively spliced at the fifth exon, deleting approximately one-third of the c-terminal activation domain. among the different muscle types examined, visceral smooth muscles have a very low ratio of srfdelta5 to srf. increased levels of srf ...	1999	10373507
cloning and functional expression of a cdna encoding a metabolic acyl-coa delta 9-desaturase of the cabbage looper moth, trichoplusia ni.	acyl-coa delta 9-desaturases play essential roles in fatty acid metabolism and the regulation of cell membrane fluidity. in this research, a cdna sequence was obtained from trichoplusia ni adult fat body mrna by using rt-pcr with degenerate primers based on other characterized delta 9-desaturase sequences. the remainder of the sequence was amplified using 3'- and 5'-race. a 1439 bp cdna reconstructed from three overlapping pcr products contains an orf encoding a 353-amino acids (aa) protein that ...	1999	10380655
edg-2 in myelin-forming cells: isoforms, genomic mapping, and exclusion in charcot-marie-tooth disease.	edg-2 is an heptahelical receptor whose spatio-temporal distribution during rat brain development is consistent with a role in the control of myelination. we have now identified two splice variants of edg-2 mrna in rat brain that encode two receptor isoforms differing by a stretch of 18 amino acids in the nh2-terminal extracellular tail of the receptor. prenatally (i.e., before oligodendrocyte myelination), the two variants detected by selective in situ hybridization are equally abundant, vary i ...	1999	10384882
highly divergent amino termini of the homologous human alr and yeast scerv1 gene products define species specific differences in cellular localization.	the yeast scerv1 gene product is involved in the biogenesis of mitochondria and is indispensable for viability and regulation of the cell cycle. recently the general importance of this gene for the eukaryotic cell was shown by the identification of a structural and functional human homologue. the homologous mammalian alr (augmenter of liver regeneration) genes from man, mouse and rat are involved in the phenomenon of liver regeneration. a low expression rate of the genes is found in all investig ...	1999	10384986
drosophila roadblock and chlamydomonas lc7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis.	eukaryotic organisms utilize microtubule-dependent motors of the kinesin and dynein superfamilies to generate intracellular movement. to identify new genes involved in the regulation of axonal transport in drosophila melanogaster, we undertook a screen based upon the sluggish larval phenotype of known motor mutants. one of the mutants identified in this screen, roadblock (robl), exhibits diverse defects in intracellular transport including axonal transport and mitosis. these defects include intr ...	1999	10402468
bcr binds to the xeroderma pigmentosum group b protein.	the bcr gene is involved in the formation of the bcr-abl oncogene responsible for the pathogenesis of philadelphia chromosome-positive human leukemias. we have previously shown that p210 bcr-abl binds to the xeroderma pigmentosum group b protein (xpb) through the portion of bcr that is homologous to the catalytic domain of gdp-gtp exchangers such as yeast cdc24 and dbl. in the baculovirus overexpression system which facilitates binding of coexpressed proteins, we now show that xpb binds to the i ...	1999	10403766
interaction of the alpha(1b)-adrenergic receptor with gc1q-r, a multifunctional protein.	gc1q-r, a multifunctional protein, was found to bind with the carboxyl-terminal cytoplasmic domain of the alpha(1b)-adrenergic receptor (173 amino acids, amino acids 344-516) in a yeast two-hybrid screen of a cdna library prepared from the rat liver. in a series of studies with deletion mutants in this region, the ten arginine-rich amino acids (amino acids 369-378) were identified as the site of interaction. the interaction was confirmed by specific co-immunoprecipitation of gc1q-r with full-len ...	1999	10409668
cell differentiation increases astrocyte phagocytic activity. a quantitative analysis of both gfap labeling and pas-stained yeast cells.	since efficiency of phagocytic potential in activated astrocytes is still a subject of controversy, an attempt was made to quantify simultaneously phagocytic activity and astrocyte differentiation. resorting to junin virus, known to induce astrocyte activation, infected vs control samples of cultured rat astroglial cells were serially harvested up to day 12 post-inoculation (pi), and subjected to a triple staining procedure consisting in immunoperoxidase labeling of gfap, periodic acid-schiff (p ...	1999	10413896
avirulence of candida albicans cahk1 mutants in a murine model of hematogenously disseminated candidiasis.	deletion of both alleles of the candida albicans cahk1 gene, which causes cells to flocculate when grown at ph 7.5, a ph comparable to that of mammalian blood, abolishes the ability of the yeast to establish a successful infection in a murine model of hematogenously disseminated candidiasis. within 72 h all mice inoculated with the parental c. albicans strain had died. the mice infected with either the heterozygote or revertant strain, either of which harbors only one functional cahk1 allele, al ...	1999	10417206
aps, an adapter protein with a ph and sh2 domain, is a substrate for the insulin receptor kinase.	aps (adapter protein with a ph and sh2 domain) is the newest member of a family of tyrosine kinase adapter proteins including sh2-b and lnk. we previously identified sh2-b as an insulin-receptor-binding protein and substrate [kotani, wilden and pillay (1998) biochem j. 335, 103-109]. here we show that aps interacts with the insulin receptor kinase activation loop through its sh2 domain and insulin stimulates the tyrosine-phosphorylation of aps. furthermore, the phosphorylation of activation-loop ...	1999	10417330
characterization of a multicopy family of genes encoding a surface-expressed serine endoprotease in rat pneumocystis carinii.	a unique family of genes encoding serine endoproteases related to the saccharomyces cerevisiae serine endoprotease kexin was identified in pneumocystis carinii. unlike previously described serine endoprotease genes that are single copies, multiple copies of the p. carinii serine endoprotease are distributed throughout the genome. the proteins predicted by these variant genes demonstrate sequence variability, but they retain the conserved active sites associated with endoprotease activity. the se ...	1999	10417743
involvement of crm1, a nuclear export receptor, in mrna export in mammalian cells and fission yeast.	crm1, an evolutionarily conserved protein, was shown to be a receptor for leucine-rich nuclear export signal (nes)-dependent protein transport. in lower eukaryotes crm1 is reported to be required for the export of mrna, however, involvement of the nes-dependent transport pathway in mrna export in higher eukaryotes has not been established.	1999	10421839
differential changes in alpha2-macroglobulin and hemopexin in brain and liver in response to acute inflammation.	changes in serum and cerebrospinal fluid (csf) proteins following generalized acute inflammation induced by fermented yeast in the rat was examined by concanavalin a-blotting, immunoblotting, and radioimmunoassay. using alpha2-macroglobulin (alpha2-m) and hemopexin (hpx) as marker proteins, the concentration alpha2-m was found to increase in serum and csf by 150- and 5-fold, respectively, whereas the concentration of hpx increased by about 4-fold in both fluids following yeast-induced inflammati ...	1999	10424910
synthesis of furanonaphthoquinones with hydroxyamino side chains.	several furanonaphthoquinones have shown useful activity in a yeast assay for dna-damaging agents and cytotoxicity in mammalian cell culture assays. these results, together with the planar aromatic character of the furanonaphthoquinones, suggested that they might be acting as dna intercalators. in an attempt to improve this activity, various analogues containing a hydroxyamino side chain have been synthesized. the analogues were prepared by standard methods, but some unexpected reactions were ob ...	1999	10425117
new applications of low-c0tdna as a dna fingerprint probe.	new applications of low-c0t dna are reported as probes for genetic identification and genome characterization. these fast and intermediately reannealing fractions have sometimes either been discarded in genomic library construction to enhance the probability of finding single copy genes, or they are used as resources for identifying individual repetitive sequences. in addition, they are used as blockers to enhance hybridization signals. c0t-1 dna serves as a probe for dna fingerprinting of human ...	1999	10435445
mammalian chk2 is a downstream effector of the atm-dependent dna damage checkpoint pathway.	in response to dna damage and replication blocks, cells activate pathways that arrest the cell cycle and induce the transcription of genes that facilitate repair. in mammals, atm (ataxia telangiectasia mutated) kinase together with other checkpoint kinases are important components in this response. we have cloned the rat and human homologs of saccharomyces cerevisiae rad 53 and schizosaccharomyces pombe cds1, called checkpoint kinase 2 (chk2). complementation studies suggest that chk2 can partia ...	1999	10435585
expression of human papillomavirus 16 e2 protein in schizosaccharomyces pombe delays the initiation of mitosis.	infection by some types of human papillomavirus (hpv) is associated with the development of cervical cancer. analysis of viral dna from cervical tumours shows that the e2 gene is frequently disrupted during integration into the host cell's dna. it has therefore been suggested that loss of e2p is an important step in malignant transformation. expression of e2p in the fission yeast schizosaccharomyces pombe retards the g2-m transition, by delaying activation of cdc2p kinase. in contrast, s phase p ...	1999	10435625
exo84p is an exocyst protein essential for secretion.	the exocyst is a multiprotein complex that plays an important role in secretory vesicle targeting and docking at the plasma membrane. here we report the identification and characterization of a new component of the exocyst, exo84p, in the yeast saccharomyces cerevisiae. yeast cells depleted of exo84p cannot survive. these cells are defective in invertase secretion and accumulate vesicles similar to those in the late sec mutants. exo84p co-immunoprecipitates with the exocyst components, and a por ...	1999	10438536
aberrant interactions of transcriptional repressor proteins with the huntington's disease gene product, huntingtin.	we detected an interaction of the n-terminus of huntingtin (htt171) with the c-terminal region of the nuclear receptor co-repressor (n-cor) using the yeast two-hybrid system. this interaction was repeat length dependent and specific to htt171; the co-repressor did not interact with the repeat carrying a section of atrophin 1 nor with the androgen receptor or polyglutamine alone. the interaction was confirmed using his-tagged escherichia coli -expressed c-terminal human and rat co-repressor prote ...	1999	10441327
the significance and mechanism of mitochondrial proton conductance.	there is a futile cycle of pump and leak of protons across the mitochondrial inner membrane. the contribution of the proton cycle to standard metabolic rate is significant, particularly in skeletal muscle, and it accounts for 20% or more of the resting respiration of a rat. the mechanism of the proton leak is uncertain: basal proton conductance is not a simple biophysical leak across the unmodified phospholipid bilayer. equally, the evidence that it is catalysed by homologues of the brown adipos ...	1999	10454114
competitive interaction of seven in absentia homolog-1a and ca2+/calmodulin with the cytoplasmic tail of group 1 metabotropic glutamate receptors.	group 1 metabotropic glutamate receptors (mglur1 and mglur5) are coupled to inositol trisphosphate/ca2+ signaling via g proteins and play an important role in excitatory synaptic transmission. to explore the regulation of group 1 mglur function, we applied the yeast two-hybrid system using the intracellular carboxy-terminal domain of group 1 mglurs (group 1 ct-mglurs) and attempted to identify novel protein-protein interactions of group 1 mglurs.	1999	10469171
export pumps for anionic conjugates encoded by mrp genes.	several members of the multidrug resistance protein (mrp) family mediate the atp-dependent transport of amphiphilic anions across membranes. the substrate specificity of recombinant human mrp1 has been most extensively defined by use of inside-out membrane vesicles. substrates include the glutathione s-conjugate leukotriene c4, 17 beta-glucuronosyl estradiol, glucuronosyl bilirubin, glutathione disulfide, in addition to the fluorescent lipophilic anion fluo-3. these substances are also substrate ...	1999	10470375
human very long-chain acyl-coa synthetase and two human homologs: initial characterization and relationship to fatty acid transport protein.	several human genes with a high degree of homology to rat very long-chain acyl-coa synthetase (rvlcs) and mouse fatty acid transport protein (mfatp) were identified. full-length cdna clones were obtained for three genes, and predicted amino acid sequences were generated. initial characterization indicated that one gene was most likely hvlcs, the human ortholog of rvlcs. the other two (hvlcs-h1 and hvlcs-h2) were more closely related to rvlcs than to mfatp. phylogenetic analysis of amino acid seq ...	1999	10471116
expression profile of the copper homeostasis gene, ratox1, in the rat brain.	in humans the regulation of cellular copper homeostasis is essential for proper organ development and function. a novel cytosolic protein, named atox 1, was recently identified in yeast that functions in shuttling intracellular mononuclear copper [cu(i)] to copper-requiring proteins. atox 1 and its human homolog, hatox1, are members of an emerging family of proteins termed copper chaperones that are involved in the maintenance of copper homeostasis. northern blot analysis demonstrates that atox ...	1999	10473283
identification of an nadh-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, mortierella alpina 1s-4, by sequencing of the encoding cdna and heterologous expression in a fungus, aspergillus oryzae.	based on the sequence information for bovine and yeast nadh-cytochrome b(5) reductases (cbrs), a dna fragment was cloned from mortierella alpina 1s-4 after pcr amplification. this fragment was used as a probe to isolate a cdna clone with an open reading frame encoding 298 amino acid residues which show marked sequence similarity to cbrs from other sources, such as yeast (saccharomyces cerevisiae), bovine, human, and rat cbrs. these results suggested that this cdna is a cbr gene. the results of a ...	1999	10473389
rat tumour necrosis factor-alpha: expression in recombinant pichia pastoris, purification, characterization and development of a novel elisa.	tumour necrosis factor-alpha is a pro-inflammatory cytokine involved in many aspects of acute phase and immune responses. species specificity in the biological action and receptor binding of tnf-alpha make it desirable to use homologous reagents in experimental models, both in vivo and in vitro. as the rat is the model of choice in many investigations on fever, trauma and pathology, there is a need for specific rat reagents. in this paper, we describe the production of recombinant rat tnf-alpha ...	1999	10477395
human liver-specific very-long-chain acyl-coenzyme a synthetase: cdna cloning and characterization of a second enzymatically active protein.	activation of fatty acids, catalyzed by acyl-coenzyme a (acyl-coa) synthetases, is required for their subsequent metabolism. peroxisomes and microsomes contain very-long-chain acyl-coa synthetases (vlcss) capable of activating fatty acids with a chain length of 22 or more carbons. decreased peroxisomal vlcs activity is, in part, responsible for the biochemical pathology in x-linked adrenoleukodystrophy (x-ald), illustrating the importance of vlcss in cellular fatty acid homeostasis. we previousl ...	1999	10479480
sertolin is a novel gene marker of cell-cell interactions in the rat testis.	a novel testicular protein designated sertolin was cloned. the full-length sertolin cdna consists of 853 base pairs with an open reading frame of 381 base pairs coding for a 127-amino acid polypeptide that shares limited identities with antaxin/josephin and thrombospondin proteins. sertolin (calculated molecular mass, 13,759 daltons) has two mrna transcripts of 2.3 and 1 kilobase. a 22-amino acid peptide based on the deduced amino acid sequence of sertolin (nh(2)-kkehfnlfkaasvshlvqvvpq) was synt ...	1999	10480919
molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids.	to study the putative role of human carnitine octanoyltransferase (cot) in the beta-oxidation of branched-chain fatty acids, we identified and cloned the cdna encoding human cot and expressed it in the yeast saccharomyces cerevisiae. enzyme activity measurements showed that cot efficiently converts one of the end products of the peroxisomal beta-oxidation of pristanic acid, 4, 8-dimethylnonanoyl-coa, to its corresponding carnitine ester. production of the carnitine ester of this branched/medium- ...	1999	10486279
cooperation of six and eya in activation of their target genes through nuclear translocation of eya.	drosophila sine oculis and eyes absent genes synergize in compound-eye formation. the murine homologues of these genes, six and eya, respectively, show overlapping expression patterns during development. we hypothesized that six and eya proteins cooperate to regulate their target genes. cotransfection assays were performed with various combinations of six and eya to assess their effects on a potential natural target, myogenin promoter, and on a synthetic promoter, the thymidine kinase gene promo ...	1999	10490620
emk protein kinase-null mice: dwarfism and hypofertility associated with alterations in the somatotrope and prolactin pathways.	gene trapping was used in embryonic stem (es) cells in an attempt to inactivate genes involved in development. the emk (elkl motif kinase) gene has been disrupted and a mutant mouse line derived. previous work had shown that emk kinases, called mark in the rat, exert a major control on microtubule stability by phosphorylating microtubule-associated proteins and that genes homologous to emk in yeast or caenorhabditis elegans are essential for cell and embryonic polarity. although we found the emk ...	1999	10491259
cloning and expression of ctp:phosphoethanolamine cytidylyltransferase cdna from rat liver.	ctp:phosphoethanolamine cytidylyltransferase (et) is a key regulatory enzyme in the cdp-ethanolamine pathway for phosphatidylethanolamine synthesis. as a first step in the elucidation of the structure-function relationship and the regulation of et, an et cdna was cloned from rat liver. the cloned cdna encodes a protein of 404 amino acid residues with a calculated molecular mass of 45.2 kda. the deduced amino acid sequence is very similar to that of human et (89% identity). furthermore, it shows ...	1999	10493918
chicken ovalbumin upstream promoter-transcription factor ii, a new partner of the glucose response element of the l-type pyruvate kinase gene, acts as an inhibitor of the glucose response.	transcription of the l-type pyruvate kinase (l-pk) gene is induced by glucose in the presence of insulin and repressed by glucagon via cyclic amp. the dna regulatory sequence responsible for mediating glucose and cyclic amp responses, called glucose response element (glre), consists of two degenerated e boxes spaced by 5 base pairs and is able to bind basic helix-loop-helix/leucine zipper proteins, in particular the upstream stimulatory factors (usfs). from ex vivo and in vivo experiments, it ap ...	1999	10497199
reconstitution of fibroblast growth factor receptor interactions in the yeast two hybrid system.	fibroblast growth factors (fgf) activate their receptors through the formation of trimolecular complexes, composed of a ligand, a receptor, and a heparan sulfate oligosaccharide, all of which are members of particularly large families capable of multiple interactions in a combinatorial fashion. understanding this large network of interactions not only presents a great challenge, but is practically beyond the capacity of most classical techniques routinely used to study ligand receptor interactio ...	1999	10503237
disruption of six orfs on saccharomyces cerevisiae chromosome x: the yjl069c gene of unknown function is essential to cell viability.	the short flanking homology pcr strategy (wach et al., 1994) was used to disrupt six open reading frames (orfs) on chromosome x of diploid strains (fy1679 and w303) of the yeast saccharomyces cerevisiae. two of the six orfs analysed (yjl069c and yjl066c) display no similarity to known sequences. three others (yjl065c, yjl068c, and yjl070c) are similar to those respectively encoding the dna polymerase epsilon subunit c, human esterase d and rat amp deaminase 1. yjl071w has recently been identifie ...	1999	10509023
a functional and quantitative mutational analysis of p53 mutations in yeast indicates strand biases and different roles of mutations in dmba- and bbn-induced tumors in rats.	in order to analyze the mutational events and to understand the biological significance of the p53 gene in chemical carcinogenesis, we applied a new yeast-based p53 functional assay to ovarian tumors induced by 7, 12-dimethylbenz[a]anthracene (dmba), as well as to transitional cell carcinomas of the urinary bladder induced by n-butyl-n-(4-hydroxybutyl) nitrosamine (bbn) in rats. the assay demonstrated that 15 of 19 dmba induced tumors harbored clonal p53 mutations, which is consistent with the e ...	1999	10521810
vap-33 localizes to both an intracellular vesicle population and with occludin at the tight junction.	tight junctions create a regulated intercellular seal between epithelial and endothelial cells and also establish polarity between plasma membrane domains within the cell. tight junctions have also been implicated in many other cellular functions, including cell signaling and growth regulation, but they have yet to be directly implicated in vesicle movement. occludin is a transmembrane protein located at tight junctions and is known to interact with other tight junction proteins, including zo-1. ...	1999	10523508
interaction of the metalloprotease disintegrins mdc9 and mdc15 with two sh3 domain-containing proteins, endophilin i and sh3px1.	metalloprotease disintegrins (a disintegrin and metalloprotease (adam) and metalloprotease, disintegrin, cysteine-rich proteins (mdc)) are a family of membrane-anchored glycoproteins that function in diverse biological processes, including fertilization, neurogenesis, myogenesis, and ectodomain processing of cytokines and other proteins. the cytoplasmic domains of adams often include putative signaling motifs, such as proline-rich sh3 ligand domains, suggesting that interactions with cytoplasmic ...	1999	10531379
heterologous gene expression in a membrane-protein-specific system.	we have constructed an expression system for heterologous proteins which uses the molecular machinery responsible for the high level production of bacteriorhodopsin in halobacterium salinarum. cloning vectors were assembled that fused sequences of the bacterio-opsin gene (bop) to coding sequences of heterologous genes and generated dna fragments with cloning sites that permitted transfer of fused genes into h. salinarum expression vectors. gene fusions include: (i) carboxyl-terminal-tagged bacte ...	1999	10545281
biochemical characterization and expression analysis of neural thrombospondin-1-like proteins nell1 and nell2.	two closely related genes coding for nell proteins (nell1 and nell2) have been cloned by the yeast two-hybrid screening of a rat brain cdna library with the regulatory domain of protein kinase c betai (pkcbetai) as bait. the rat nell proteins show about 55% identity with each other and contain several protein motifs assigned to a secretion signal peptide, an nh(2)-terminal thrombospondin-1 (tsp-1)-like module, five von willebrand factor c domains, and six epidermal growth factor-like domains; th ...	1999	10548494
identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity.	p21-activated kinases (pak)/ste20 kinases are regulated in vitro and in vivo by the small gtp-binding proteins rac and cdc42 and lipids, such as sphingosine, which stimulate autophosphorylation and phosphorylation of exogenous substrates. the mechanism of pak activation by these agents remains unclear. we investigated pak kinase activation in more detail to gain insight into the interplay between the gtpase/sphingosine binding, an intramolecular inhibitory interaction, and autophosphorylation. w ...	1999	10551809
molecular cloning of rat atx1 homologue protein.	an atx1 homologue of 503 bp length was cloned from a rat cdna library, and the deduced protein from the cdna was found to contain 68 amino acids with a predicted molecular mass of 7.2 kda. the rat atx1 homologue protein (rah1p), which shows 35%, 38%, and 89% identities with atx1p, cuc-1, and hah1, respectively, conserves both the mtcxxc copper-binding site in the n terminus and the ktgk lysine-rich region in the c terminus. in northern blot analysis, rah1 mrna was found to be expressed at high l ...	1999	10558899
receptor-independent activators of heterotrimeric g-protein signaling pathways.	heterotrimeric g-protein signaling systems are activated via cell surface receptors possessing the seven-membrane span motif. several observations suggest the existence of other modes of stimulus input to heterotrimeric g-proteins. as part of an overall effort to identify such proteins we developed a functional screen based upon the pheromone response pathway in saccharomyces cerevisiae. we identified two mammalian proteins, ags2 and ags3 (activators of g-protein signaling), that activated the p ...	1999	10559191
functional analysis of aquaporin-2 mutants associated with nephrogenic diabetes insipidus by yeast expression.	mutations of aquaporin-2 (aqp2) vasopressin water channel cause nephrogenic diabetes insipidus (ndi). it has been suggested that impaired routing of aqp2 mutants to the plasma membrane causes the disease; however, no determinations have been made of mutation-induced alterations of aqp2 channel water permeability. to address this issue, a series of aqp2 mutants were expressed in yeast, and the osmotic water permeability (p(f)) of the isolated vesicles was measured. wild-type and mutant aqp2 were ...	1999	10564236
suppression of microphthalmia transcriptional activity by its association with protein kinase c-interacting protein 1 in mast cells.	microphthalmia (mi) is a transcription factor that plays a major role in the regulation of growth and function in mast cells and melanocytes. association of mi with other proteins is a critical step in the regulation of mi-mediated transcriptional activation. we found protein kinase c-interacting protein 1 (pkci) specifically associated with mi in yeast two-hybrid screening. immunoprecipitation of mi from quiescent rat basophilic leukemic cells or mouse melanocytes resulted in the specific co-im ...	1999	10567402
specific interaction of the 70-kda heat shock cognate protein with the tetratricopeptide repeats.	using a yeast two-hybrid system with the 70-kda heat shock cognate protein (hsc70) or its c-terminal 30-kda domain as baits, we isolated several proteins interacting with hsc70, including hip/p48 and p60/hop. both are known to interact with hsc70. except for hip/p48, all of the proteins that we isolated interact with the 30-kda domain. moreover, the eevd motif at the c terminus of the 30-kda domain appears essential for this interaction. sequence analysis of these hsc70-interacting proteins reve ...	1999	10567422
neural restrictive silencer factor recruits msin3 and histone deacetylase complex to repress neuron-specific target genes.	accumulative evidence suggests that more than 20 neuron-specific genes are regulated by a transcriptional cis-regulatory element known as the neural restrictive silencer (nrs). a trans-acting repressor that binds the nrs, nrsf [also designated re1-silencing transcription factor (rest)] has been cloned, but the mechanism by which it represses transcription is unknown. here we show evidence that nrsf represses transcription of its target genes by recruiting msin3 and histone deacetylase. transfect ...	1999	10570134
the novel kinase peptidylglycine alpha-amidating monooxygenase cytosolic interactor protein 2 interacts with the cytosolic routing determinants of the peptide processing enzyme peptidylglycine alpha-amidating monooxygenase.	the cytosolic domain of the peptide-processing integral membrane protein peptidylglycine alpha-amidating monooxygenase (pam; ec 1.14. 17.3) contains multiple signals determining its subcellular localization. three pam cytosolic interactor proteins (p-cips) were identified using the yeast two hybrid system (alam, m. r., caldwel, b. d., johnson, r. c., darlington, d. n., mains, r. e., and eipper, b. a. (1996) j. biol. chem. 271, 28636-28640); the partial amino acid sequence of p-cip2 suggested tha ...	1999	10574929
yeast smf1 mediates h(+)-coupled iron uptake with concomitant uncoupled cation currents.	yeast membrane proteins smf1, smf2, and smf3 are homologues of the dct1 metal ion transporter family. their functional characteristics and the implications of these characteristics in vivo have not yet been reported. here we show that smf1 expressed in xenopus oocytes mediates h(+)-dependent fe(2+) transport and uncoupled na(+) flux. smf1-mediated fe(2+) transport exhibited saturation kinetics (k(m) = 2.2 microm), whereas the na(+) flux did not, although both processes were electrogenic. smf1 is ...	1999	10574989
from yeast to man--from mitochondria to liver regeneration: a new essential gene family.	the purpose of this review is to bring to the attention of the reader the latest developments in research on an important new emerging gene family. the respective genes are found in eukaryotes from yeast to man and even on the genome of some doubled-stranded dna viruses. they have essential functions in the biogenesis of mitochondria, the cell division cycle and, in higher eukaryotes, in the development of organs like liver and testis. the most important medical implication is their probable rol ...	1999	10575569
the profile of fr140423, a novel anti-inflammatory compound, in yeast-induced rat hyperalgesia.	the mechanism of action of fr140423 (3-(difluoromethyl)-1-(4-methoxyphenyl)-5-[4-(methylsulfinyl)phenyl]pyra zole), a novel anti-inflammatory compound, in a rat yeast-induced hyperalgesic model was investigated and compared with those of indomethacin and morphine. we tested the inhibitory effects of fr140423 on the formation of arachidonic acid metabolites, prostaglandin (pg) e2, thromboxane (tx) b2 and leukotriene (lt) b4, in yeast-injected inflamed paws and the effect of the opioid receptor an ...	1999	10580376
the dynamin-like protein dlp1 is essential for normal distribution and morphology of the endoplasmic reticulum and mitochondria in mammalian cells.	the dynamin family of large gtpases has been implicated in vesicle formation from both the plasma membrane and various intracellular membrane compartments. the dynamin-like protein dlp1, recently identified in mammalian tissues, has been shown to be more closely related to the yeast dynamin proteins vps1p and dnm1p (42%) than to the mammalian dynamins (37%). furthermore, dlp1 has been shown to associate with punctate vesicles that are in intimate contact with microtubules and the endoplasmic ret ...	1999	10588666
mammalian mitochondrial ribosomal proteins (2). amino acid sequencing, characterization, and identification of corresponding gene sequences.	four different classes of mammalian mitochondrial ribosomal proteins were identified and characterized. mature proteins were purified from bovine liver and subjected to n-terminal or matrix-assisted laser-desorption mass spectroscopic amino acid sequencing after tryptic in-gel digestion and high pressure liquid chromatography separation of the resulting peptides. peptide sequences obtained were used to virtually screen expressed sequence tag data bases from human, mouse, and rat. consensus cdnas ...	1999	10593885
identification and characterization of cvhsp. a novel human small stress protein selectively expressed in cardiovascular and insulin-sensitive tissues.	starting with computational tools that search for tissue-selective expression of assembled expressed sequenced tags, we have identified by focusing on heart libraries a novel small stress protein of 170 amino acids that we named cvhsp. cvhsp was found as being computationally selectively and highly (0.3% of the total rna) expressed in human heart. the cvhsp gene mapped to 1p36.23-p34.3 between markers d1s434 and d1s507. the expression of cvhsp was analyzed with rna dot, northern blots, or revers ...	1999	10593960
interaction partners of dlk/zip kinase: co-expression of dlk/zip kinase and par-4 results in cytoplasmic retention and apoptosis.	dlk/zip kinase is a newly discovered serine/threonine kinase which, due to its homology to dap kinase, was named dap like kinase, dlk. this kinase is tightly associated with nuclear structures, it undergoes extensive autophosphorylation and phosphorylates myosin light chain and core histones h3, h2a and h4 in vitro. moreover, it possesses a leucine zipper which mediates interaction with transcription factor atf-4, therefore it was called zip kinase. we employed the yeast two-hybrid system to ide ...	1999	10602480
comparison in metabolic activity of cytochrome p450 1a1 on heterocyclic amines between human and rat.	in mutagenicity and antimutagenicity tests, the toxicants have been activated to the ultimate mutagenic forms usually with rat cytochrome p450 (cyp) enzymes. an understanding is important of whether these data can be available for human. in this paper are compared the activating abilities of cyp1a1 between human and rat using recombinant yeast cells that express respective cyp1a1 and yeast nadph-cyp-oxidoreductase simultaneously. three different types of dietary procarcinogens, heterocyclic amin ...	1999	10606558
apobec-1 dependent cytidine to uridine editing of apolipoprotein b rna in yeast.	cytidine to uridine editing of apolipoprotein b (apob) mrna requires the cytidine deaminase apobec-1 as well as a tripartite sequence motif flanking a target cytidine in apob mrna and an undefined number of auxiliary proteins that mediate rna recognition and determine site-specific editing. yeast engineered to express apobec-1 and apob mrna supported editing under conditions of late log phase growth and stationary phase. the cis -acting sequence requirements and the intracellular distribution of ...	2000	10606639
adenovirus type 5 e1a immortalizes primary rat cells expressing wild-type p53.	adenovirus (ad) e1a induces apoptosis in cells expressing wild-type p53, and stable transformation by ad e1a requires the co-introduction of an anti-apoptotic gene such as ad e1b 19k. thus, cells immortalized by ad e1a alone might have lost functional p53. in order to analyze the p53 in rat cells expressing ad e1a, we established rat cell lines by transfecting primary rat embryo fibroblast (ref) and baby rat kidney (brk) cells with cloned ad5 e1a. by using a yeast functional assay, we analyzed p ...	1999	10609613
the essential cofactor trrap recruits the histone acetyltransferase hgcn5 to c-myc.	the c-myc protein functions as a transcription factor to facilitate oncogenic transformation; however, the biochemical and genetic pathways leading to transformation remain undefined. we demonstrate here that the recently described c-myc cofactor trrap recruits histone acetylase activity, which is catalyzed by the human gcn5 protein. since c-myc function is inhibited by recruitment of histone deacetylase activity through mad family proteins, these opposing biochemical activities are likely to be ...	2000	10611234
yotiao protein, a ligand for the nmda receptor, binds and targets camp-dependent protein kinase ii(1).	a yeast two-hybrid screen revealed that regulatory subunits (rii) of pkaii bind the yotiao protein. yotiao interacts with the nr1 subunit of the nmda receptor. a purified c-terminal fragment of yotiao binds pkaii, via an rii binding site constituted by amino acid residues 1452-1469, with a dissociation constant (k(d)) between 50 and 90 nm in vitro. a stable complex composed of yotiao, rii and nr1 was immunoprecipitated from whole rat brain extracts. immunostaining analysis disclosed that yotiao, ...	1999	10618500
nxp-1, a human protein related to rad21/scc1/mcd1, is a component of the nuclear matrix.	nuclear matrix is a complex intranuclear network supposed to be involved in the various nuclear functions. in order to identify the nuclear matrix proteins, we isolated a cdna clone from a human placenta cdna library. this clone was partially represented a known cdna clone ha1237. ha1237 encoded a 631-amino-acid peptide, which we designated nxp-1. nxp-1 was related to yeast rad21/scc1/mcd1, xenopus xrad21, and mouse pw29, and identical with hr21spa isolated from a human testis cdna library. we d ...	2000	10623634
an african swine fever virus erv1-alr homologue, 9gl, affects virion maturation and viral growth in macrophages and viral virulence in swine.	the african swine fever virus (asfv) genome contains a gene, 9gl, with similarity to yeast erv1 and alr genes. erv1 has been shown to function in oxidative phosphorylation and in cell growth, while alr has hepatotrophic activity. 9gl encodes a protein of 119 amino acids and was highly conserved at both nucleotide and amino acid levels among all asfv field isolates examined. monospecific rabbit polyclonal antibody produced to a glutathione s-transferase-9gl fusion protein specifically immunopreci ...	2000	10627538
neuronal inwardly rectifying k(+) channels differentially couple to pdz proteins of the psd-95/sap90 family.	several signaling proteins clustered at the postsynaptic density specialization in neurons harbor a conserved c-terminal pdz domain recognition sequence (x-s/t-x-v/i) that mediates binding to members of the psd-95/sap90 protein family. this motif is also present in the c termini of some inwardly rectifying k(+) (kir) channels. constitutively active kir2 channels as well as g protein-gated kir3 channels, which are fundamental for neuronal excitability, were analyzed as candidates for binding to p ...	2000	10627592
protein inhibitor of activated stat-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis.	an androgen receptor (ar) interacting protein was isolated from a hela cell cdna library by two-hybrid screening in yeast using the ar dna+ligand binding domains as bait. the protein has sequence identity with human protein inhibitor of activated signal transducer and activator of transcription (pias1) and human gu rna helicase ii binding protein (gbp). binding of pias1 to human ar dna+ligand binding domains was androgen dependent in the yeast liquid beta-galactosidase assay. activation of bindi ...	2000	10628744
the rna interacting domain but not the protein interacting domain is highly conserved in ribosomal protein p0.	protein p0 interacts with proteins p1alpha, p1beta, p2alpha, and p2beta, and forms the saccharomyces cerevisiae ribosomal stalk. the capacity of rpp0 genes from aspergillus fumigatus, dictyostelium discoideum, rattus norvegicus, homo sapiens, and leishmania infantum to complement the absence of the homologous gene has been tested. in s. cerevisiae w303dgp0, a strain containing standard amounts of the four p1/p2 protein types, all heterologous genes were functional except the one from l. infantum ...	2000	10636918
atpase-defective mammalian vps4 localizes to aberrant endosomes and impairs cholesterol trafficking.	the yeast vacuolar sorting protein vps4p is an atpase required for endosomal trafficking that couples membrane association to its atpase cycle. to investigate the function of mammalian vps4 in endosomal trafficking, we have transiently expressed wild-type or atpase-defective human vps4 (hvps4) in cultured cells. wild-type hvps4 was cytosolic, whereas a substantial fraction of hvps4 that was unable to either bind or hydrolyze atp was localized to membranes, including those of specifically induced ...	2000	10637304
intraperoxisomal localization of very-long-chain fatty acyl-coa synthetase: implication in x-adrenoleukodystrophy.	x-adrenoleukodystrophy (x-ald) is a demyelinating disorder characterized by the accumulation of saturated very-long-chain (vlc) fatty acids (>c(22:0)) due to the impaired activity of vlc acyl-coa synthetase (vlcas). the gene responsible for x-ald was found to code for a peroxisomal integral membrane protein (aldp) that belongs to the atp binding cassette superfamily of transporters. to understand the function of aldp and how aldp and vlcas interrelate in the peroxisomal beta-oxidation of vlc fat ...	2000	10640429
chromosomal localization of phospholipase a2 activating protein, an ets2 target gene, to 9p21.	a murine ets2 target gene isolated by differential display cloning was identified as the phospholipase a2 activating protein (plaa) gene. a 2.7-kb human cdna demonstrating high homology to mouse and rat plaa genes was then isolated and characterized. human plaa contains six wd-40 repeat motifs and three different protein kinase consensus domains. fluorescence in situ hybridization (fish) mapping placed plaa on chromosome 9p21, a region frequently deleted in various cancers. a comprehensive mappi ...	1999	10644453
identification of a human brain-specific isoform of mammalian ste20-like kinase 3 that is regulated by camp-dependent protein kinase.	a novel isoform of mammalian ste20-like kinase 3 (mst3) with a different 5' coding region from mst3, termed mst3b, was identified by searching through expressed sequence tag data base and obtained by rapid amplification of cdna 5'-ends. mst3b was assigned to the long arm of human chromosome 13, d13s159-d13s280, by use of the national center for biotechnology information sequence-tagged sites data base. reverse transcription-polymerase chain reaction and northern blot analysis with a probe derive ...	2000	10644707
molecular cloning and characterization of amida, a novel protein which interacts with a neuron-specific immediate early gene product arc, contains novel nuclear localization signals, and causes cell death in cultured cells.	amida was isolated by the yeast two-hybrid system as a novel protein which associated with arc, a non-transcriptional immediate early gene specific to the brain. amida was confirmed to be associated with arc in vitro and in vivo. amida shows no homology to known proteins. amida is ubiquitously expressed, although it is abundant in the brain. a transfection study revealed that amida was localized in the nucleus and after 72 h the transfected cells underwent apoptosis. furthermore, we found two nu ...	2000	10644725
pap i interacts with itself, pap ii, pap iii, and lithostathine/regialpha.	pap i, pap ii, pap iii, and lithostathine/regialpha are members of a multigenic family of proteins expressed in several tissues. pap i was shown to be antiapoptotic, mitogenic, and anti-inflammatory and can promote cell adhesion to the extracellular matrix. lithostathine/regialpha can be mitogenic. because polymerization might regulate activity, we examined the ability of rat pap i to interact with itself (homodimerization), pap ii, pap iii, and lithostathine/regialpha (heterodimerization) by th ...	1999	10662590
active intracellular domain of notch enhances transcriptional activation of ccaat/enhancer binding protein beta on a rat pregnancy-specific glycoprotein gene.	pregnancy-specific glycoproteins (psgs) are primarily expressed in the placenta and become the major glycoproteins at term. to understand the regulation of psg expression, we characterized the promoter elements of a rodent psg gene, rncgm3, and delineated three nuclear protein binding sites: fpi, -ii, and -iii in the 5'-flanking region of the gene. the fpii-binding factor is shown to be c/ebpbeta, which positively regulates rncgm3 expression [chen, h., et al. (1995) dna cell biol. 14, 681-688]. ...	2000	10677215
pancreatic eukaryotic initiation factor-2alpha kinase (pek) homologues in humans, drosophila melanogaster and caenorhabditis elegans that mediate translational control in response to endoplasmic reticulum stress.	in response to different cellular stresses, a family of protein kinases regulates translation by phosphorylation of the alpha subunit of eukaryotic initiation factor-2 (eif-2alpha). recently, we identified a new family member, pancreatic eif-2alpha kinase (pek) from rat pancreas. pek, also referred to as rna-dependent protein kinase (pkr)-like endoplasmic reticulum (er) kinase (perk) is a transmembrane protein implicated in translational control in response to stresses that impair protein foldin ...	2000	10677345
an electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins.	choline is an important metabolite in all cells due to the major contribution of phosphatidylcholine to the production of membranes, but it takes on an added role in cholinergic neurons where it participates in the synthesis of the neurotransmitter acetylcholine. we have cloned a suppressor for a yeast choline transport mutation from a torpedo electric lobe yeast expression library by functional complementation. the full-length clone encodes a protein with 10 putative transmembrane domains, two ...	2000	10677542
conserved composition of mammalian box h/aca and box c/d small nucleolar ribonucleoprotein particles and their interaction with the common factor nopp140.	small nucleolar ribonucleoprotein particles (snornps) mainly catalyze the modification of rrna. the two major classes of snornps, box h/aca and box c/d, function in the pseudouridylation and 2'-o-methylation, respectively, of specific nucleotides. the emerging view based on studies in yeast is that each class of snornps is composed of a unique set of proteins. here we present a characterization of mammalian snornps. we show that the previously characterized nap57 is specific for box h/aca snornp ...	2000	10679015
dissecting the interactions between ntf2, rangdp, and the nucleoporin xfxfg repeats.	we have used a range of complementary biochemical and biophysical methods to investigate the interactions between nuclear transport factor 2 (ntf2), the ras family gtpase ran, and xfxfg nucleoporin repeats that are crucial for nuclear trafficking. microcalorimetry, microtiter plate binding, and fluorescence quenching in solution are all consistent with the binding constant for the ntf2-rangdp interaction being in the 100 nm range, whereas the interaction between ntf2 and xfxfg repeat-containing ...	2000	10681579
identification of a brain specific protein that associates with a refsum disease gene product, phytanoyl-coa alpha-hydroxylase.	refsum disease is an autosomal recessive neurologic disorder of the lipid metabolism. major diagnostic clinical findings include retinitis pigmentosa, peripheral polyneuropathy, cerebellar ataxia, increased cerebrospinal fluid protein without pleocytosis, nerve deafness, and cardiac involvement. we have identified a novel protein (pahx-ap #1) associated with phytanoyl-coa alpha-hydroxylase (pahx), a refsum disease gene product, using the yeast-based two-hybrid assay. the middle portion (amino ac ...	2000	10686344