Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the prevalence of cervical infection with human papillomaviruses and cervical dysplasia in alaska native women. | alaska native women historically have high rates of sexually transmitted diseases (stds) and invasive cervical cancer. their prevalence of cervical infections with human papillomavirus (hpv) in relation to cervical dysplasia was determined with a commercial dot hybridization test for seven hpv genotypes. type-specific hpv dna, similarly distributed between genotype groups 16/18 and 31/33/35, was detected in 234 cervical specimens (21%) from 1126 alaska native women seeking routine care and colpo ... | 1994 | 8133094 |
| a virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16. | previous studies have demonstrated that genital infection with high-risk types of human papillomavirus (hpv), most often hpv16, is the most significant risk factor for the development of cervical cancer. however, serologic assays that have been developed to identify high-risk hpv infection have either failed to associate serum reactivity with other indicators of hpv infection or have identified only a minority of hpv-infected individuals. | 1994 | 8133532 |
| prevalence in sweden of human papillomavirus type 16 infection in cin ii-iii cases assessed by a pcr method. | the presence of human papillomavirus (hpv) 16 in cervical intraepithelial neoplasia (cin)ii-iii cases was studied by means of a polymerase chain reaction (pcr)-based method applied to paraffin-embedded tissues. twenty-two of 45 (49%) cases showed a positive signal determined by direct visualization on gel stained with ethidium bromide. fourteen of these cases were confirmed by dot blot hybridization, which accords with a prevalence of hpv 16 infection in 31% of stockholm patients with cin ii-iii ... | 1993 | 8139214 |
| adult rhabdomyoma. a light microscopic, ultrastructural, virologic, and immunologic analysis. | adult rhabdomyoma is a rare benign tumor of skeletal muscle origin that frequently arises from musculature of the head and neck region. in this study we report five cases of adult rhabdomyoma. light and electron microscopic examination revealed the typical cellular features as described by others. the results of immunophenotyping and quantitation of nucleolar organizer regions were consistent for a benign skeletal muscle tumor. epstein-barr virus and human papillomavirus genomic dna could not be ... | 1994 | 8139832 |
| 1993 sexually transmitted diseases treatment guidelines. centers for disease control and prevention. | these guidelines for the treatment of patients with sexually transmitted diseases (stds) were developed by staff members of cdc after consultation with a group of invited experts who met in atlanta on january 19-21, 1993. included are new recommendations for single-dose oral therapy for gonococcal infections, chlamydial infections, and chancroid; new regimens for the treatment of bacterial vaginosis (bv) and outpatient management of pelvic inflammatory disease (pid); a new patient-applied medica ... | 1993 | 8145706 |
| cationic liposomes mediated delivery of antisense oligonucleotides targeted to hpv 16 e7 mrna in caski cells. | the "high risk" types 16 and 18 of human papillomavirus (hpv) are involved in the etiology of genital squamous cell carcinoma. the early genes 6 and 7 (e6-e7) of these viruses code for the major transforming proteins, capable of inducing cell transformation alone or acting synergistically with other oncogenes. antisense oligonucleotides, recently applied to inhibit the functions of a number of cellular and viral proteins, might provide the basis for a new therapeutic strategy against hpv-induced ... | 1994 | 8147581 |
| sequence variation in the capsid protein genes of human papillomavirus type 16. | we have cloned and sequenced the l1 and l2 genes from human papillomavirus type 16 (hpv16) dna-containing cervical cytology samples collected from the u.k. and trinidad. samples containing high copy numbers of hpv16 dna were selected as being likely to contain fully functional virus dna molecules in an episomal state, rather than in an integrated and possibly altered state. in comparison with the previously published sequence of hpv16 isolated from an invasive cancer a variety of differences wer ... | 1994 | 8151305 |
| [analysis of p53 gene in gynecologic tumors]. | the inactivation of the tumor suppressor gene p53 has been demonstrated in a variety of human tumors. herein, we performed a p53 gene analysis of human gynecologic tumor cell lines and tumor tissues. in the sk-ov-3 cell line, southern analysis suggested the presence of sequence deletions/rearrangements in at least one allele of p53 gene. transcripts were not detectable by either northern or pcr analysis. sequencing analysis of the entire coding region revealed mutations changing the p53 amino ac ... | 1994 | 8152158 |
| local epidermal viral infections: comparative aspects of vaccinia virus, herpes simplex virus and human papillomavirus in man and orf virus in sheep. | 1994 | 8156048 | |
| human cytotoxic t lymphocytes stimulated by endogenously processed human papillomavirus type 11 e7 recognize a peptide containing a hla-a2 (a*0201) motif. | cytotoxic t lymphocytes (ctl) may play an important role in the control of human papillomavirus (hpv)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. to address this we have stimulated human hpv-specific ctl in vitro using low-density cells (ldc) from peripheral blood mononuclear cells (pbmc). low-density cells were used to present synthetic peptides, or endogenously processed peptides expressed from ... | 1994 | 8157270 |
| detection of human papillomaviruses in paraffin-embedded biopsies of cervical intraepithelial lesions: analysis by immunohistochemistry, in situ hybridization, and the polymerase chain reaction. | one hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (cil) were tested for the presence of human papillomavirus (hpv) by immunohistochemistry and in situ hybridization using commercial biotinylated probes (vira-type in situ assay; digene diagnostics, silver spring, md) or probes labeled with digoxigenin by the random primer technique. immunohistochemistry was more inferior to the in situ hybridization method, with a detection rate of 14% (20/140) compared t ... | 1994 | 8159640 |
| absence of human papilloma virus dna in oropharyngeal spindle-cell squamous carcinomas. | the presence of human papilloma virus (hpv) dna in verrucous carcinomas and some squamous-cell carcinomas (scc) of the oropharynx suggests a role for the virus in their pathogenesis. spindle-cell squamous carcinoma is an uncommon variant of oropharyngeal scc. its association with the hpv has not been reported. in situ and dot blot hybridization, using biotinylated probes to hpv 6, 11, 16, 18, 31, 33, and 35, failed to detect hpv dna in nine studied cases of spindle-cell squamous carcinoma, as di ... | 1994 | 8160645 |
| the biology of human papillomaviruses: from warts to cancer. | papillomaviruses are small dna viruses that induce a variety of proliferative lesions in most mammals, including humans. of the 66 types of human papillomaviruses (hpvs) that have been identified, a subset that includes types 16, 18, 31, 33, and 51 is associated frequently with anogenital cancers. these cancers develop from precursor lesions, which, for cervical cancer, are termed cervical intraepithelial neoplasias (cin), and are graded from i to iii depending on the degree of disruption of epi ... | 1993 | 8162357 |
| detection of human papillomavirus in squamous intraepithelial lesions by consensus and type-specific polymerase chain reaction. | polymerase chain reaction (pcr) was used to identify human papillomavirus (hpv) in 216 cervical biopsy specimens from women referred to the gynecological out-patient unit for colposcopy because of an abnormal smear. hpv dna was screened using type-specific primers for hpv6, 11, 16, 18, 31 and 33 (ts-pcr) as well as a consensus primer located in the e1 region of the hpv genome (c-pcr). ts-pcr specificity was validated by southern blot analysis. low-grade (sil 1) and high-grade (sil 2) squamous in ... | 1993 | 8163035 |
| tracheobronchial involvement of laryngeal papillomatosis at onset. | a case of adult onset laryngeal papillomas with tracheal and bronchial involvement present at onset is presented. the presence of human papilloma virus (hpv) dna type 6/11 is demonstrated by in situ hybridization. | 1994 | 8163923 |
| the absence of vaginal human papillomavirus 16 dna in women who have not experienced sexual intercourse. | to determine whether young women who have not experienced sexual intercourse may harbor genital human papillomavirus (hpv) infection in the vaginal-ectocervical mucosa. | 1994 | 8164934 |
| the state of the p53 gene in human papillomavirus (hpv)-positive and hpv-negative genital precancer lesions and carcinomas as determined by single-strand conformation polymorphism analysis and sequencing. | human papillomavirus (hpv) is frequently associated with cervical carcinoma. inactivation of the p53 tumor suppressor gene product by binding to the hpv encoded e6 protein is considered as an important pathway for malignant progress in hpv-infected cells. in contrast, mutations of the p53 gene have been found in hpv-negative cervical carcinoma cells. to evaluate the involvement of p53 inactivation for the development of genital carcinoma, we determined the state of the p53 gene in 20 genital pre ... | 1994 | 8166446 |
| human papillomavirus and epstein-barr virus in the etiology of testicular germ cell tumours. | epidemiological features suggest that the risk of testicular cancer may be related to exposure to unknown infectious agents, including viruses. therefore a series of twenty specimens of testicular germ cell tumours, including preinvasive carcinoma in-situ, were tested for the presence of dna sequences of two viruses with known transforming abilities, human papillomavirus (hpv) and epstein-barr virus (ebv). the polymerase chain reaction (pcr) technique was used. in none of the 19 successfully amp ... | 1994 | 8166998 |
| activation of eukaryotic transcriptional promoters by the bovine papillomavirus e1-replication factor. | it has been suggested that the bovine papillomavirus type 1 e1 replication factor may inhibit e2-conditional gene expression from viral promoter p89. to study the possible role of the e1 protein in gene expression, hela cells were transfected with e2-conditional or e2-independent reporter plasmids and with vectors expressing the e1 and e2 open reading frames. the data show that: (i) replication factor e1 stimulates gene expression from a variety of eucaryotic transcriptional promoters; (ii) acti ... | 1993 | 8169116 |
| prevalence of anal human papillomavirus infection and intraepithelial neoplasia in renal allograft recipients. | a study was performed to test the hypothesis that renal allograft recipients are at high risk of developing anal human papillomavirus (hpv) infection and anal intraepithelial neoplasia (ain). a total of 133 renal allograft recipients and 145 control patients underwent anoscopy and biopsy. a polymerase chain reaction was used to detect hpv16 dna in biopsy samples. a histological diagnosis of anal hpv infection or ain was made in 32 allograft recipients (hpv infection, five; ain i, 20; ain ii, thr ... | 1994 | 8173899 |
| interferon and retinoic acid suppress the growth of human papillomavirus type 16 immortalized cervical epithelial cells, but only interferon suppresses the level of the human papillomavirus transforming oncogenes. | in the present study, we examine the effects of all-trans-retinoic acid (ra) and interferons-alpha and -gamma (ifn-alpha and ifn-gamma) on the growth of hpv16-immortalized cell lines, ece16-1 and caski. treating proliferating ece16-1 cells with ra causes a concentration-dependent decrease in cell number. at 1 microm ra, cell growth is suppressed by 65% and the level of mrna encoding cytokeratin k5, a biochemical marker of retinoid action, is also suppressed. in contrast, the level of transcript ... | 1994 | 8174114 |
| analysis of hpv-positive and -negative vulvar carcinomas for alterations in c-myc, ha-, ki-, and n-ras genes. | mutation or overexpression of certain host genes, including c-myc, ha-ras, and ki-ras, have been associated with genital squamous neoplasia, specifically in the cervix, and have been implicated in the natural history of these tumors. the relationship of these host gene alterations to vulvar squamous cell carcinomas has not been previously studied. we analyzed archival material from 13 human papillomavirus-positive and -negative vulvar squamous cell carcinomas for mutations in ha-, ki-, and n-ras ... | 1994 | 8175026 |
| fast solid support detection of human papillomavirus in in vitro amplified dna using a dnp-anti dnp monoclonal antibody couple. | in some anogenital lesions the detection of certain types of human papilloma virus, especially oncogenic types, is of interest. in a first step during a prospective study, we compared two methods for the detection of human papillomavirus (hpv) dna in clinical samples: southern blotting followed by hybridization with a cloned radioactive genomic probe and a classical polymerase chain reaction (pcr) followed by hybridization with a 32p-labelled oligonucleotide probe. 118 biopsies and swabs were ex ... | 1994 | 8175945 |
| stomach cancer in transgenic mice expressing human papillomavirus type 16 early region genes from a keratin promoter. | certain human papillomaviruses (hpvs) have been implicated as important contributory factors in the development of cervical carcinoma and other epithelial malignancies. in order to investigate the role of papillomavirus early gene expression in epithelial oncogenesis in vivo, we produced transgenic mice expressing hpv-16 early region genes from the promoter of the bovine keratin 6 gene. spliced transcripts were detected in the tongue, forestomach, glandular stomach, female reproductive tract and ... | 1994 | 8176373 |
| development of a broad spectrum pcr assay for papillomaviruses and its application in screening lung cancer biopsies. | a pcr assay was developed to detect known and as yet unidentified papillomaviruses (pvs). for this purpose we analysed the conserved amino acid sequences in the l1 and e1 open reading frames of 45 human and nine animal pvs. candidate regions for the design of a primer were identified as those having the least number of amino acid and nucleotide sequence variants among the different pvs. these regions in the l1 orf have been described previously. we modified the sequences of the backward and the ... | 1994 | 8176375 |
| assembly of the major and the minor capsid protein of human papillomavirus type 33 into virus-like particles and tubular structures in insect cells. | native virions of human papillomaviruses (hpv) can be isolated from genital lesions only in very limited amounts. recent studies have shown that virus-like particles can be obtained by expression of the capsid proteins using vaccinia virus recombinants or the baculovirus system. we now present the first detailed characterization of virus-like particles of a human papillomavirus associated with malignant genital lesions, hpv-33, produced in high yield using the baculovirus expression system. asse ... | 1994 | 8178438 |
| cervical neoplasia in the patient with hiv infection. | with heterosexual transmission of hiv becoming the primary mode of transmission to women in the usa, and a high rate of human papillomavirus (hpv) and cervical neoplasia in hiv-infected women, obstetrician-gynecologists have become primary care providers for hiv-infected women. reports of a high rate of recurrence and progression of cervical neoplasia in this population suggest that gynecologists must strive to identify those women with cervical neoplasia who are hiv infected. alterations in loc ... | 1994 | 8180357 |
| association between hla-dqb1 alleles and risk for cervical cancer in african-american women. | squamous-cell carcinoma of the cervix and its precursor lesions are associated with human papillomavirus (hpv) infection. epidemiological studies indicate that hpv infection in itself is not sufficient for cervical-cancer induction, suggesting that other factors contribute to carcinogenesis. we have investigated the potential role of host genetic background as one such factor. we screened a series of squamous-cell carcinomas of the cervix for hla-class-ii dqb1* alleles by the polymerase chain re ... | 1994 | 8181853 |
| genital human papillomavirus (hpv) infection in hungarian women. | the prevalence of genital human papillomavirus (hpv) infection in hungarian female populations is not essentially different from that found in other countries of europe and north-america. using filter in situ hybridization (fish), we found that, in a group of cytologically normal women some low risk hpv types (such as hpv 6 and 11) and the most important high risk hpv types (hpv 16 and 18) were present in 23% and 8%, respectively. eighty-eight percent of condyloma acuminatum patients harboured h ... | 1993 | 8184666 |
| human papillomavirus dna in adenocarcinoma and adenosquamous carcinoma of the uterine cervix detected by polymerase chain reaction (pcr). | although human papillomaviruses (hpv) have been implicated strongly in the pathogenesis of squamous cell carcinomas of the uterine cervix, a few studies have suggested that hpv may be an etiological factor for adenocarcinoma of the cervix. to evaluate the association between hpv infection and cervical adenocarcinoma, we analyzed formalin-fixed, paraffin-embedded tissues from 64 patients with adenocarcinoma or adenosquamous carcinoma of the uterine cervix for the presence of hpv dna using polymer ... | 1994 | 8188078 |
| erythrocyte folate levels, oral contraceptive use and abnormal cervical cytology. | the initial hypothesis of this study was that folate depletion is a risk factor for human papillomavirus infection and cervical epithelial cell abnormalities, including dysplasia. the prevalences of low erythrocyte folate levels (defined as < 140 ng/ml erythrocytes and determined by the growth of lactobacillus) were measured in 250 university of california at los angeles students. among oral contraceptive users, low erythrocyte folate was a risk factor for an abnormal cytologic smear in both ben ... | 1994 | 8191820 |
| lack of detection of human papillomavirus (hpv) in transformed laryngeal keratoses by in situ hybridization (ish) technique. | laryngeal keratosis (lk) is a precancerous mucosal change with a variable possibility of malignant transformation. recent studies evidencing hpv-dna genomes in a large series of non-malignant and malignant laryngeal lesions suggest a role of hpv in the transformation of laryngeal lesions possibly in synergistic interaction with other carcinogens. in this study, we analyzed 115 biopsy specimens from benign laryngeal lesions to evaluate the risk of malignant transformation and its relationship to ... | 1994 | 8203204 |
| malignant anal tumours. | anal tumours represent 5 per cent of anorectal cancers and exist as two clinical entities: tumours of the anal canal and those of the anal margin. smoking and sexual behaviour, particularly homosexual anal intercourse, are important aetiological factors. this association is related to anal warts and human papillomavirus infection, notably type 16, which is found in around 70 per cent of warts. symptoms are non-specific and are frequently attributed to benign conditions. rectal examination reveal ... | 1994 | 8205420 |
| primer-directed sequencing of human papillomavirus types. | 1994 | 8205838 | |
| epidemiology of cervical human papillomavirus infections. | 1994 | 8205846 | |
| the absence of human papilloma virus (hpv) related parameters in sexually non-active women. | in to order to investigate the prevalence of hpv related parameters in sexually non-active women, smears of 75 virgins were microscopically reviewed for alterations caused by hpv i.e. koilocytosis, parakeratosis and multinucleinization. in none of these smears were the mentioned parameters seen together, so the diagnosis of hpv infection could not be assessed. in an age-matched control population consisting of 75 sexually active women smears were reviewed. in this group 10 smears (13%) were foun ... | 1994 | 8206070 |
| human papillomavirus infections of the genital region in human immunodeficiency virus seropositive women: integration of type 16 correlates with rapid progression. | the efficacy of treatment of hpv infections was evaluated in 14 hiv-1 seropositive women. disease evolution was evaluated at follow-up examinations, and findings were compared to those in 14 historic hiv-negative women who had received similar treatment. hpv sequences were demonstrated by southern blot in 13/14 hiv-positive women and by in situ hybridization in 5/13 hiv-negative women. types 6/11, 16, and 31/33/35 were equally distributed in both groups; type 18 was never detected. in all but on ... | 1994 | 8206072 |
| interaction of hpv e6 with p53 and associated proteins. | 1994 | 8206279 | |
| inhibition of p53 dna binding by human papillomavirus e6 proteins. | transformation by the human papillomavirus (hpv) early gene products, e6 and e7, involves their interaction with cellular proteins p53 and rb. using glutathione s-transferase (gst) fusion proteins, we found that hpv e6 bound human p53 and that the relative efficiency of binding varied such that the gst-hpv type 16 e6 (16e6) protein bound p53 with highest affinity, followed by gst-31e6, gst-18e6, and gst-11e6. the gst-e6 fusion proteins were sufficient for binding p53 purified from a baculovirus ... | 1994 | 8207801 |
| three-dimensional structure of vaccinia virus-produced human papillomavirus type 1 capsids. | the capsid proteins of papillomavirus self-assemble to form empty capsids or virus-like particles that appear quite similar to naturally occurring virions by conventional electron microscopy. to characterize such virus-like particles more fully, cryoelectron microscopy and image analysis techniques were used to generate three-dimensional reconstructions of capsids produced by vaccinia virus recombinants (v capsids) that expressed human papillomavirus type 1 l1 protein only or both l1 and l2 prot ... | 1994 | 8207824 |
| papillomavirus genomes in human cervical carcinoma: analysis of their integration and transcriptional activity. | eighty-four biopsies derived from cervical tissues were analyzed for the presence of human papillomavirus (hpv) dna types 6, 16 and 18 using southern blot hybridization. hpv 6 was found in none of the cervical biopsies, and hpv types 16 and 18 were found in 44% of them. the rate of hpv 16/18 positive samples increased proportionally to the severity of the lesion. in normal tissue there were no positive samples, in mild and moderate dysplasias hpv 16/18 was present in 20% and in severe dysplasias ... | 1994 | 8208320 |
| detection of human papilloma virus (hpv) infection in paraffin-embedded tissues of endometrial carcinoma. | although human papilloma virus (hpv) associated lesions constitute a well recognized clinical entity in the female lower genital tract, namely vulva, vagina and cervix, few studies have demonstrated hpv infection in other genital sites, particularly in the ovary and uterine corpus. recently, with the highly sensitive polymerase chain reaction (pcr) technique, hpv infections were found in an ovarian tumour and adenocarcinoma of the cervix. this prompted a retrospective analysis of hpv dna in 22 c ... | 1993 | 8216121 |
| what is vestibular papillomatosis? a study of its prevalence, aetiology and natural history. | to clarify the prevalence, aetiology, symptoms and natural history of vestibular papillomatosis. | 1993 | 8217979 |
| anogenital warts in prepubertal children; sexual abuse or not? | forty-two prepubertal children presenting with anogenital (ag) warts (15 boys and 27 girls) were prospectively followed up (mean 15.9 months, sd 12.24). most (73.8%) of these children had perianal condylomatous-type warts and 11 (26.2%) had concurrent non-genital (ng) warts. none had any other ag infections or sexually transmitted disease (std). twelve (28.6%) children acquired their ag warts by vertical transmission from an infected maternal birth canal, 3 (7.1%) by autoinoculation from common ... | 1993 | 8218514 |
| risk factors for cervical intraepithelial neoplasia grade iii/carcinoma in situ in spain and colombia. | a case-control study of 525 histologically confirmed cases of cervical intraepithelial neoplasia grade iii and 512 controls was done in spain and colombia to assess the role of various risk factors taking into account the effect of human papillomavirus (hpv). the presence of hpv dna, assessed by a polymerase chain reaction-based method, was the strongest risk factor identified. in spain the adjusted odds ratio (or) and 95% confidence interval (ci) (numbers in parentheses) were 56.9 (24.8-130.6) ... | 1993 | 8220086 |
| the hpv-16 e6 and e6-ap complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. | the ubiquitin-dependent proteolytic pathway plays a major role in selective protein degradation. ubiquitination of proteins requires the sequential action of the ubiquitin-activating enzyme (e1), ubiquitin-conjugating enzymes (e2), and in some cases ubiquitin-protein ligases (e3s). the oncogenic human papillomavirus (hpv) types 16 and 18 utilize this cellular proteolytic system to target the tumor suppressor protein p53. the hpv e6 oncoprotein binds to a cellular protein of 100 kd, termed e6-ass ... | 1993 | 8221889 |
| [condyloma acuminatum: histopathology and detection of human papilloma virus]. | comparing with the results of hpv dna detection by pcr, 44 cases of condyloma acuminatum and condyloma-like lesions in female genital tract were studied histopathologically. among 40 cases formerly diagnosed as condyloma acuminatum or suspicious condyloma acuminatum, all were hpv dna detectable. hpv6 and hpv11 were found in 4 cases without koilocytes. hpv6 was found in one out of 4 cases formerly diagnosed as pseudocondyloma. the result showed that koilocytes are characteristic and diagnostic, a ... | 1993 | 8221990 |
| immunohistochemical detection of mutant p53 protein and human papillomavirus-related e6 protein in anal cancers. | the wild-type p53 protein, a product of the p53 gene, is a normal growth controlling protein. mutation of the p53 gene generates a mutant p53 protein which promotes tumor formation through loss of growth suppression. some of the agents responsible for p53 gene mutation are known, one of which may be tumorigenic human papillomavirus (hpv) infection. anal cancers are demonstrating a changing trend in the affected population, from older females in the older reported series to younger males more rec ... | 1993 | 8223054 |
| translational properties of the human papillomavirus type-6 l1-coding mrna. | a cdna encoding a bicistronic mrna, e1e4l1, which was generated by double-splicing of the e1, e4 and l1 genes, of the type-6 human papillomavirus (hpv-6), was cloned. the e1e4 and l1 open reading frames (orfs) in this cdna were expressed in cos-1 or cv-1 cells as fusion proteins with escherichia coli beta-galactosidase (beta gal), and the products were analyzed by immunoprecipitation and enzyme assay. the results showed that the translational efficiency of the l1 orf was about 9-15-fold less eff ... | 1993 | 8224910 |
| simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to pcr for human papillomavirus detection. | sample preparation is an important step in the detection of viral dna by the polymerase chain reaction (pcr) technique. the method used should achieve release of cellular dna with the minimum of manipulation steps so as to reduce the possibility of contamination. the present report demonstrates that either microwaving or 20 min of boiling in the heating block of a thermal cycler lead to satisfactory results in the detection by pcr of human papillomavirus in cervicovaginal epithelial cell specime ... | 1993 | 8227280 |
| detection of human papillomavirus infection by non-isotopic in situ hybridisation in condylomatous and cin lesions. | to study the value of non-isotopic in situ hybridisation (nish) in detecting human papillomavirus (hpv) infection in female genital lesions positive for the virus by conventional histology but negative by filter dna hybridisation. | 1993 | 8227412 |
| [cancer of the cervix, papillomavirus, contraception and tobacco]. | this survey started in may 1989 and deals with 2,436 patients treated by 10 gynecologists from the brussels region. in addition to routine cytological analysis in cervical smears, an additional test for human papilloma virus (hpv 16, 18 and 33) was advised for these women. gynecologists filled in a questionnaire about these patients. in cases with benign cytology, virus prevalence was 2 times greater in woman who took contraceptive pills, over at least 6 months, than in each of the other groups ... | 1993 | 8228010 |
| analysis of p107-associated proteins: p107 associates with a form of e2f that differs from prb-associated e2f-1. | the binding of viral oncogenes to cellular proteins is thought to modulate the activities of these cellular targets. the p107 protein is targeted by many viral proteins, including adenovirus e1a, simian virus 40 large t antigen, and human papillomavirus type 16 e7 protein. a panel of monoclonal antibodies against p107 was raised and used to identify cellular proteins that interact with the p107 protein in vivo. p107-associated proteins included cyclin a, cyclin e, and cdk2. in addition, p107 was ... | 1993 | 8230483 |
| white lichenoid lesions of the buccal mucosa in patients with hiv infection. | we report on eight patients who developed white lichenoid lesions of the buccal mucosa during the course of human immunodeficiency virus infection. in five patients the lesions appeared after the administration of zidovudine, in two after the intake of both zidovudine and ketoconazole, and in one after ketoconazole. in the majority of cases, lesions presented as bilateral reticular keratosis or atrophic changes of the buccal mucosa. three patients manifested lichenoid atrophic changes of the dor ... | 1993 | 8233426 |
| detection of human papillomavirus deoxyribonucleic acid sequences in amniotic fluid during different periods of pregnancy. | 1993 | 8238123 | |
| p53 gene mutations and mdm2 amplification are uncommon in primary carcinomas of the uterine cervix. | the p53 gene is the most frequently altered gene known thus far in a wide variety of human cancers. inactivation of p53, either through mutation or through interaction with the human papillomavirus (hpv) e6 oncoprotein, is a characteristic feature of all cervical carcinoma cell lines that have been studied. these findings suggest that p53 inactivation is required for cervical carcinoma development and that hpv infection and p53 mutation may be mutually exclusive. we have studied the p53 gene in ... | 1993 | 8238255 |
| the association between sexually transmitted pathogens and cervical intra-epithelial neoplasia in a developing community. | objective--to determine the association of sexually transmitted pathogens in women with cervical intra-epithelial neoplasia (cin). setting--an urban tertiary referral hospital serving a large indigent developing community. participants--48 women attending a colposcopy clinic and 49 women attending a family planning clinic. methods--vaginal, endocervical, rectal swab specimens and sera were collected for the detection of sexually transmitted pathogens. cervical cytology was performed on all patie ... | 1993 | 8244352 |
| serologic response in human papillomavirus-associated invasive cervical cancer. | human papillomavirus (hpv) transforming proteins e6 and e7 are uniformly expressed in hpv-associated cervical cancer. our objective was to measure antibodies to hpv-16 e6 and e7 proteins in cervical cancer patients using an assay which would detect antibodies to conformational epitopes. serum specimens obtained from two case-control studies of hpvs and cervical cancer were tested. the studies were performed in cali, colombia, south america and in 9 provinces of spain. cases consisted of women wi ... | 1993 | 8244575 |
| human papillomavirus type 1 dna in verrucous carcinoma of the leg. | 1993 | 8245239 | |
| trans-activation of the adenovirus e2 promoter by human papillomavirus type 16 e7 is mediated by retinoblastoma-dependent and -independent pathways. | in common with the adenovirus e1a and simian virus 40 large t oncoproteins, the e7 protein of human papillomavirus (hpv) type 16 interacts with the retinoblastoma (rb) tumour suppressor protein (prb). the functional importance of this interaction for hpv-16 e7 protein was investigated by analysis of the transactivating function of e7 at the adenovirus e2 promoter in a set of breast tumour cell lines. trans-activation by hpv-16 e7 in two prb-deficient cell lines demonstrated that prb is not essen ... | 1993 | 8245866 |
| double infection with human papillomavirus 1 and human papillomavirus 63 in single cells of a lesion displaying only an human papillomavirus 63-induced cytopathogenic effect. | specific histologic features are associated with the presence of certain human papillomaviruses (hpv) in verrucous lesions of the skin. hpv 1 is known to be associated with eosinophilic keratohyalin-like intracytoplasmic inclusion bodies, granular in appearance, whereas the recently described hpv 63 seems to induce inclusion bodies displaying a filamentous structure. although an infection by two distinct hpv types within a single cell has not been previously reported, we have recently treated a ... | 1993 | 8246450 |
| detection of human immunodeficiency virus type 1 and human papillomavirus type 16 in acquired immunodeficiency syndrome-related and classic kaposi's sarcoma. | 1993 | 8250588 | |
| analysis of human papillomavirus types in exophytic condylomata acuminata by hybrid capture and southern blot techniques. | exophytic condylomata acuminata of the external genitalia of 40 patients were analyzed for human papillomavirus (hpv) dna by the southern blot and hybrid capture methods. all lesions were initially analyzed by the southern blot method by using a mixture of hpv type 6, 11, 16, and 18 whole genomic probes. southern blots demonstrated characteristic psti restriction patterns of hpv type 6, 11, or 16 in all but one lesion. hpv 6 subtypes accounted for 28 of 39 hpv-positive lesions. twenty-seven of t ... | 1993 | 8253963 |
| [detection of hpv infection in cin and invasive cancers by in situ hybridization method--comparison with histopathology]. | the causal association of human papilloma virus (hpv) with cervical cancer has been supported by multiple lines of evidence. therefore, in the case of dysplasia, the presence of hpv-dna should be detected and its subtypes identified. this is important in the determination of the prognosis for cervical disease. we reported a study in which the localization and types of hpv in cervical diseases was identified by in situ hybridization using biotin-labelled dna probes. seven types (3 basic forms) of ... | 1993 | 8254963 |
| a population-based seroepidemiological study of cervical cancer. | the epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (hpv) type 16 or 18. we performed a seroepidemiological study of hpv and cervical cancer in the counties of västerbotten and norrbotten in northern sweden, a low-risk area for cervical cancer. sera from 94 cases of incident cervical cancer were matched against 188 age- and sex-matched controls derived from a population-based blood ... | 1994 | 8261434 |
| massive precorneal extension of squamous cell carcinoma of the conjunctiva. | a 73-year-old man had only light perception vision from a slowly enlarging fleshy papillomatous mass covering the entire cornea. the lesion was excised completely, leaving a clear corneal bed and 20/25 vision. histopathologically, the lesion was found to be squamous cell carcinoma in situ with early microinvasion of the basement membrane. viral studies for human papilloma virus were negative. this case represents a massive superficial involvement of the cornea by squamous cell carcinoma and show ... | 1993 | 8261787 |
| prevalence of serum igg antibodies for the e7 and l2 proteins of human papillomavirus type 16 in cervical cancer patients and controls. | relationship between the prevalence of the antibodies to hpv16e7 and l2 proteins and the development of cervical cancer was examined. sera from 57 patients with invasive cervical cancer and from 200 age-matched healthy blood donors (16 to 64 years old) were examined for antibodies against e7 and l2 proteins of human papillomavirus (hpv) type 16. bacterially expressed fusion antigens were used in a western immunoblot assay. ten (18%) of the patients and 10 (5%) of the controls were positive for o ... | 1993 | 8266324 |
| [detection of human papillomavirus dna in condyloma acuminatum of the urethra: a case report]. | a case in a 35-year-old male with recurrence of the condyloma acuminatum of the urethra is presented and the involvement of human papilloma virus (hpv) with this disease is mentioned. the hpv-dna in the excised specimen was detected by the dot blot hybridization method with radiolabelled rna probes, and the type of the dna was investigated by the southern blot hybridization method with biotinylated dna probes, which could detect the hpv-dna of types 6, 11, 16, 18, 31, 33 and 35. these analyses d ... | 1993 | 8266864 |
| [interferon in anogenital infections with human papillomavirus]. | interferons (ifn) provide a systemic alternative for the therapy of human papillomavirus (hpv) induced anogenital warts. local therapy cannot totally eliminate latent viral genome in the perilesional epithelium. this causes the danger of new exacerbations. reviewing different reports and by own therapeutic experience we think, that ifn treatment may be successful in combination with local therapy and for pre-surgical diminution of extended condyloma. sufficient dose is necessary. poor effects ar ... | 1993 | 8273374 |
| confirmation of the association of human papillomavirus with human colon cancer. | the human papillomavirus (hpv) has been shown to be associated with neoplasms of the human colon using immunohistochemistry and in situ hybridization. we now report our use of the polymerase chain reaction and southern blotting to investigate that same association. we selected 38 carcinomas, 21 adenomas, and 24 normal mucosal samples for the current study. tissue sections were prepared, and then dna was extracted and subjected to 40 cycles of amplification using thermus aquaticus dna polymerase ... | 1993 | 8273860 |
| case-control study of risk factors for cervical squamous-cell neoplasia in denmark. iii. role of oral contraceptive use. | the role of oral contraceptive (oc) use in relation to the risk of cervical neoplasia (squamous cell) was investigated in a population-based case-control study in denmark of 586 women with histologically verified cervical carcinoma in situ (cis), 59 women with invasive cervical cancer from copenhagen, and 614 controls drawn at random from the female population in the study area. ever use of ocs was associated with an increased crude risk for carcinoma in situ (relative risk [rr] = 1.8, 95 percen ... | 1993 | 8280828 |
| laser treatment of urethral condyloma: a five-year experience. | twenty cases of men treated with the neodymium:yttrium-aluminum-garnet (nd:yag) laser for meatal and/or urethral condyloma over five years were reviewed. | 1994 | 8284887 |
| confronting the hiv epidemic in asia and the pacific: developing successful strategies to minimize the spread of hiv infection. | in asia, the cumulative total of hiv-infected adults will reach 1.22 million by 1995, and, by 2000, the number is estimated to reach 11-45 million. the modes of transmission vary from country to country and include injecting drug users, commercial sex workers and their clients, commercial blood donors, hemophiliacs, and homosexuals. social, cultural, and health factors also affect transmission, such as rites of passage to adulthood, lack of female autonomy, multiple sex partners, wars and civi ... | 1993 | 8286067 |
| contraception choice and sexually transmitted disease. | sexually active couples need to be concerned with the risk of sexually transmitted diseases (stds) and how their choice of contraception influences that risk. condoms provide the best documented protection against such pathogens as: gonorrhea, herpes simplex virus (hsv), hepatitis b, hiv, and chlamydia. female dependent barrier methods also provide protection against most stds and also possibly hiv. most hormonal non-barrier contraceptives, although providing excellent protection against unwante ... | 1993 | 8286696 |
| a comparison of uterine cervical cytology and biopsy results: indications and outcomes for colposcopy. | there is some debate in the literature over the proper approach to the patient with a mildly abnormal cervical cytologic finding. one current approach for handling low-grade cytologic abnormalities is to perform colposcopy and biopsy if atypia, human papillomavirus (hpv) changes, or mild dysplasia is noted on cytologic examination. if a papanicolaou (pap) smear shows inflammation without atypia, the test is repeated after 3 months, and if inflammation does not clear, colposcopy is performed. thi ... | 1994 | 8289050 |
| human papillomavirus type 11 e2 proteins repress the homologous e6 promoter by interfering with the binding of host transcription factors to adjacent elements. | the e6 promoter of human papillomaviruses (hpvs) trophic for epithelia for the lower genital tract and the upper respiratory tract is regulated in vitro by homologous and heterologous papillomaviral e2 proteins that bind to a consensus responsive sequence (e2-rs) accn6ggt. when hpv type 11 (hpv-11) expression is examined in epithelial cell lines, the hpv-11 e2-c protein, which lacks the amino-terminal transactivating domain of the full-length e2 protein, invariably represses the homologous viral ... | 1994 | 8289341 |
| interaction of human papillomavirus (hpv) type 16 capsid proteins with hpv dna requires an intact l2 n-terminal sequence. | encapsidation of papillomavirus dna involves dna-protein and protein-protein interactions. we sought to define the role of each human papillomavirus (hpv) capsid protein in hpv dna encapsidation. hpv16 major (l1) and minor (l2) capsid proteins purified from recombinant vaccinia virus-infected cells were compared for their ability to bind nucleic acids. l2 protein, but not l1 protein, could bind hpv dna. to map the dna-binding region of l2, a series of truncated or point-mutated l2 protein open r ... | 1994 | 8289365 |
| human cancer cell lines express a negative transcriptional regulator of the interferon regulatory factor family of dna binding proteins. | members of the interferon regulatory factor (irf) family of dna binding transcription factors have roles in growth regulation, antiviral responses, and transcriptional induction of interferon (ifn)-activated early response genes. the irf family member isgf3 gamma is the dna binding component of ifn-stimulated gene factor 3 (isgf3), a multicomponent complex responsible for the stimulation of ifn-alpha-responsive genes. ifn-alpha-stimulated formation of isgf3 and subsequent gene expression can be ... | 1994 | 8289823 |
| dna damage induced p53 mediated transcription is inhibited by human papillomavirus type 18 e6. | cervical cancer is similar to other human cancers in that it develops through a multistep process. however, infection with oncogenic human papillomaviruses (hpvs) is believed to be essential for the initiation of this disease. although hpv may play a central role in the early stages of neoplasia, the accumulation of mutations in an assortment of genes precedes the development of malignant cervical carcinoma. the mechanisms by which abnormalities accumulate are various, but it is possible that vi ... | 1994 | 8290274 |
| molecular biology of human papillomavirus. | the papillomavirus is at present considered to be an independent group of viruses, possibly an independent family. human papillomavirus (hpv) can cause warts of the skin and mucosa, and is now widely recognized as an important etiologic factor of both benign and malignant tumors in the lower genital tract. in this paper the biological properties of hpv, including its genomic organization and the open reading frames, are described; the relationship between hpv and uterine cervical carcinoma, espe ... | 1993 | 8292796 |
| pseudo oral hairy leukoplakia in a renal allograft recipient. | oral hairy leukoplakia (ohl) is a disorder of the tongue associated with epstein-barr virus (ebv). ohl is seen mainly in hiv infection but is also rarely seen in the course of iatrogenic immunosuppression, especially in kidney transplantation; ohl is even more rarely seen in immunocompetent hosts. lesions that clinically and histologically mimicked ohl but were not associated with ebv were recently characterized as pseudo hairy leukoplakia. we present such a case that occurred in a renal allogra ... | 1994 | 8294586 |
| the p53 tumor suppressor gene frequently is altered in gynecologic cancers. | mutation of the p53 tumor suppressor gene, often accompanied by overexpression of mutant p53 protein, is the most frequent molecular genetic event described thus far in human cancers. in adenocarcinomas of the ovary and endometrium, p53 overexpression is seen in approximately 10% to 15% of early and 40% to 50% of advanced cancers. similar to many other types of human cancers, ovarian and endometrial cancers that overexpress p53 protein contain mutations in conserved regions of the p53 gene. thes ... | 1994 | 8296829 |
| human papillomavirus type 16 dna in oesophageal squamous cell carcinoma. | recent studies, mainly from high risk areas of oesophageal cancer (oc), have suggested a possible role of oesophageal infection with human papillomavirus (hpv) in the carcinogenesis of oc. twenty-two cases of oc (among them 20 cases of squamous cell, 1 case of verrucous and 1 case of adenoid cystic carcinoma) originating from a middle risk area of oc, were analysed by in situ hybridization (ish) and polymerase chain reaction (pcr) to demonstrate hpv infection. no evidence of hpv dna was found us ... | 1993 | 8297121 |
| human papillomavirus dna in the dermis of condyloma acuminatum. | condyloma acuminatum (ca) has high recurrence rates after local treatments. why this lesion is difficult to eradicate is unclear. one possible explanation for recurrence after superficial destructive therapy is the presence of residual human papillomavirus (hpv) in the superficial dermis beneath the treated epidermis. thirteen samples of ca were excised from 13 patients. thirteen samples of basal cell carcinoma (bcc) were studied for purposes of control. epidermis was separated from dermis by tr ... | 1993 | 8300929 |
| laryngeal verrucous carcinoma: a clinicopathologic study and detection of human papillomavirus using polymerase chain reaction. | laryngeal verrucous carcinoma (lvc) is a rare, well-differentiated variant of squamous carcinoma with a low malignant potential. human papillomavirus (hpv)-16 dna has been identified in a small number of lvc and an etiologic relationship has been suggested. a correlative clinical and molecular pathological study was performed in order to determine the prevalence and typing of hpv dna in lvc. possible associations between patient and tumor subsets, and the presence of hpv dna were also investigat ... | 1994 | 8302116 |
| p53 mutations in hpv-negative cervical carcinoma. | human papillomavirus (hpv) infection has been strongly linked to the development of cervical carcinoma. two viral oncoproteins, e6 and e7, produced by hpv, have been shown to immortalize primary human genital epithelial cells by interacting with the protein products of cellular tumor suppressor genes p53 and rb, respectively. e6 binds to the cellular p53 protein promoting p53 degradation and inactivity. this mechanism has been suggested to contribute to the oncogenesis of hpv-positive anogenital ... | 1994 | 8302581 |
| multicentric papillomavirus infection of the female genital tract. a study of morphologic pattern, possible risk factors and viral prevalence. | we have investigated 103 biopsy specimens obtained from 50 women (average 28.1 years, range 16-66) with multicentric condylomatosis of the genital tract, including the uterine cervix (48 samples), the vagina (23), and the vulva (32). each patient had at least 2 biopsies. a positive in situ hybridization for a human papilloma virus (hpv) cocktail was observed in samples from 38 patients: in these multiplicity of lesions regarded the cervix and vulva (22 cases), cervix and vagina (12), vulva and v ... | 1993 | 8302720 |
| [malignant transformation of juvenile-type laryngeal papillomatosis]. | juvenile laryngeal papillomatosis is characterized by benign budding tumors, developing from the endolaryngeal epithelium. because the evolution of these tumors is unpredictable, choice of the best therapy is difficult. we report two cases where invasive juvenile laryngeal papillomatosis, progressively colonized the trachea and bronchi. malignant transformation occurred without carcinoma predisposing factors. in both cases patients died after pulmonary dissemination. we discuss the role of the h ... | 1993 | 8304702 |
| bowen's disease of the nail bed and periungual area. a clinicopathologic analysis of seven cases. | this article describes the clinical and histologic features of seven cases of bowen's disease (bd) of the nail bed, evaluates the role of human papillomavirus in the bowenoid change, and discusses optimal therapy. | 1994 | 8304759 |
| significance of human papillomavirus-induced squamous cell carcinoma to dermatologists. | 1994 | 8304764 | |
| cervical cancer, human papillomavirus and vaccines. | hpv-16 has been strongly implicated in the aetiology of cervical cancer and pre-malignant cervical intraepithelial neoplasia. despite current technical difficulties it may soon be possible to treat these tumours with anti-hpv-16 vaccines. a prophylactic vaccine could, in theory, be developed to induce neutralizing antibodies to hpv-16 virions in genital secretions, and a therapeutic vaccine to elicit cytotoxic t-cell responses against established lesions. | 1993 | 8305362 |
| southern blot analysis of skin biopsies for human papillomavirus dna: renal allograft recipients in south-eastern queensland. | the 104 skin biopsies from 34 patients who attended a renal transplant unit in brisbane over 12 months included 40 squamous cell carcinoma (scc), 22 solar keratoses, 4 hyperkeratoses, 18 warts and 11 basal cell carcinoma (bcc). human papillomavirus (hpv) dna was identified by southern blot hybridisation using, as individual probes, purified insert dna from recombinant hpv 1, 2, 3 or 3/10, 4, 5 or 5/8, 7, 11, 16, 18 and 41 under relaxed conditions and characterised by restriction enzyme analysis ... | 1993 | 8311831 |
| detection of human papillomavirus type 16 e6/e7 transcripts in histologically cancer-free pelvic lymph nodes of patients with cervical carcinoma. | human papillomavirus (hpv) 16 e6/e7 mrnas have been detected in paraffin-embedded sections of histologically cancer-free pelvic lymph nodes from four of six patients with hpv 16-associated cervical cancer. the cdna obtained from the viral mrna by reverse transcription was amplified by the polymerase chain reaction (pcr). transcripts were present in a small but significant proportion (6/42, 14%) of the histologically negative/hpv 16 dna-positive lymph node blocks; but neither hpv 16 dna nor trans ... | 1994 | 8314141 |
| vulvar squamous cell carcinoma and papillomaviruses: indications for two different etiologies. | the vulvectomy specimens of 78 patients with vulvar squamous cell carcinoma were reviewed and examined for human papillomavirus (hpv) types 6, 11, 16, 18, and 33 by the polymerase chain reaction technique. the tumors were classified as keratinizing squamous cell carcinoma (ksc), as warty carcinoma (wc), and as basaloid carcinoma (bc). dna of hpv types 16 and 33 was found in 2/51 ksc, in 12/17 wc, and in 10/10 bc. hpv types 6, 11, and 18 were not detected. patients with wc and bc were younger, an ... | 1994 | 8314146 |
| parathyroid hormone-related protein and human papillomavirus in gynecological tumors. | the presence of parathyroid hormone-related protein (pthrp) and human papillomavirus (hpv) in a series of gynecological tumors from 131 unselected patients was examined. pthrp was localized immunohistochemically using a highly specific rabbit polyclonal anti-serum against pthrp(1-16). the results confirmed that gynecological malignancies, although rarely associated with humoral hypercalcemia of malignancy (hhm), stained for pthrp in a majority of the squamous-cell carcinomas (scc) at all sites, ... | 1994 | 8314318 |
| breast and cervical cancer screening of poor, elderly, black women: clinical results and implications. harlem study team. | our objective is to describe the clinical findings from a nurse-practitioner-based breast and cervical cancer screening program for poor, elderly, black women. we designed a cross-sectional descriptive study set at an urban public hospital medical clinic. all women 65 years of age and older were eligible to be screened. we measured these main outcomes: rates of participation, abnormal tests, and neoplasia. women were offered screening during a routine visit. of 689 women, 491 (71%) participated. ... | 1993 | 8347363 |
| interleukin-6 (il-6) functions as an autocrine growth factor in cervical carcinomas in vitro. | many factors have been implicated in the etiology of cervical neoplasia, with human papilloma virus being the latest in a long line of agents that may on their own or in combination exert various initiating and promoting effects on cervical cells, resulting in their transformation. however, for such altered cells to become invasive, it is clear that they must undergo alterations in their rate of turnover, state of differentiation, and motility. we have investigated the production of the multifun ... | 1993 | 8349158 |
| anal dysplasia in homosexual men: role of anoscopy and biopsy. | both anal squamous cell cancer and human papilloma virus (hpv) are increased in homosexual men. this study evaluates histology of internal anal abnormalities in a high-risk population of human immunodeficiency virus (hiv) seropositive and seronegative homosexual men. | 1993 | 8359638 |
| sv40 t antigen abrogates p53-mediated transcriptional activity. | recent evidence suggests that the tumor-suppressor protein p53 functions as a transcriptional regulator to control cell proliferation. an interaction with p53 is required for sv40 t antigen to transform primary cells; however, the effect of t antigen binding on p53 function is not known. in order to determine if an interaction with t antigen results in loss of p53-mediated transcriptional activity, we have used vectors expressing either a p53-gal4 fusion protein or a wild-type p53 protein in tra ... | 1993 | 8378089 |
| ras gene mutations and hpv infection are common in human laryngeal carcinoma. | to evaluate the role of ras activation and human papillomavirus (hpv) infection in laryngeal carcinoma, we analyzed tumor dna from 43 cases, including 25 primary laryngeal tumors, 12 lymph-node and one skin metastases, and 5 recurrent laryngeal carcinomas. thirteen normal laryngeal tissues and 7 benign laryngeal nodule biopsy specimens along with normal tissue surrounding laryngeal carcinoma in 2 cases were also included. the polymerase-chain-reaction technique was used to amplify dna fragments ... | 1993 | 8380055 |