Publications

development of hibit-tagged recombinant infectious bronchitis coronavirus for efficient in vitro and in vivo viral quantification.	coronaviruses (covs) are enveloped (+) ssrna viruses of veterinary and medical importance. because recombinant covs with reporter proteins fused with viral proteins are usually non-viable or unstable, a small and quantifiable epitope tag would be beneficial to cov research. in this study, we integrated the nanoluc binary technology to the reverse genetics of infectious bronchitis virus (ibv), a prototypic gammacoronavirus. the 11-amino-acid hibit tag was inserted to the spike (s) or membrane (m) ...	2020	32983065
infectious bronchitis coronavirus infection in chickens: multiple system disease with immune suppression.	in the early 1930s, infectious bronchitis (ib) was first characterized as a respiratory disease in young chickens; later, the disease was also described in older chickens. the etiology of ib was confirmed later as being due to a coronavirus: the infectious bronchitis virus (ibv). being a coronavirus, ibv is subject to constant genome change due to mutation and recombination, with the consequence of changing clinical and pathological manifestations. the potential use of live attenuated vaccines f ...	2020	32987684
efficacy of commercial polyvalent avian infectious bronchitis vaccines against chinese qx-like and tw-like strain via different vaccination strategies.	the infectious bronchitis virus (ibv) is an acute and highly contagious disease, which affects chickens of all ages. vaccination is the most important way to control this disease. nevertheless, novel variant strains are constantly reported because of the lack of proofreading capabilities of rna polymerase and high frequency of homologous rna recombination. cross-protection studies has demonstrated that the vaccines could provide great protective effects against viruses of same serotype or genoty ...	2020	32988513
research note: rapid detection of avian infectious laryngotracheitis virus with real-time fluorescence-based recombinase-aided amplification.	in this study, specific primers and fluorescent probes were designed to target the thymidine kinase (tk) gene sequence of avian infectious laryngotracheitis virus (iltv). through specificity and sensitivity tests, a real-time fluorescence-based recombinase-aided amplification (rf-raa) method for detecting iltv was established. the results showed that the method was specific and could be used to accurately detect iltv, and there was no cross-reaction with newcastle disease virus (ndv), avian infl ...	2020	32988516
a bio-safe multiple antigenic peptide (map) enzyme-linked immunoassay for the detection of antibodies to infectious bronchitis virus in chickens.	the objective of the study was to develop a bio-safe synthetic peptide elisa for the detection of antibodies against the infectious bronchitis virus (ibv) using a novel multiple antigenic peptide approach (map). after initial elisa optimization, diagnostic sensitivity (dsn) and specificity (dsp) for the linear peptides were determined using receiver operator curve (roc) analysis. the peptide ibvp1 showed 90.44% dsn and 88.64% dsp at roc cut off 22.8% while ibvp2 showed 88.24% dsn and 85.23% dsp ...	2020	32999814
development of a recombinant thermostable newcastle disease virus (ndv) vaccine express infectious bronchitis virus (ibv) multiple epitopes for protecting against ibv and ndv challenges.	newcastle disease (nd) and infectious bronchitis (ib) are two highly contagious diseases that severely threaten the poultry industry. the goal of this study is to prevent these two diseases and reduce the vaccine costs during storage and transportation. in this study, we design a thermostable recombinant newcastle disease virus (ndv) candidate live vaccine strain designated as rls-t-hn-t/b, which expresses the multiple epitope cassette of the identified infectious bronchitis virus (ibv) (s-t/b). ...	2020	33019497
phylogenetic analysis of infectious bronchitis virus circulating in southern china in 2016-2017 and evaluation of an attenuated strain as a vaccine candidate.	avian infectious bronchitis (ib) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (ibv), that poses an important economic threat to the poultry industry. in recent years, genotypes gi-7, gi-13, and gi-19 have been the most prevalent ibv strains in china. however, in this study, we found that most ibv strains from southern china in 2016-2017 belonged to genotype gvi-1. this genotype, for which there is no vaccine, has been reported sporadically in the region ...	2020	33067648
development and immunogenic potentials of chitosan-saponin encapsulated dna vaccine against avian infectious bronchitis coronavirus.	infectious bronchitis (ib) is an economically important disease of poultry that also serve as model for the understanding of other coronaviruses associated diseases. ib is considered as a major challenge to the poultry industry worldwide as a result of its effect on egg production, weight gain as well as mortality. different ibv genotypes continue to emerge, thus, the need for broad based vaccines to curb the disease. based on bioinformatic data obtained in this study, sets of monovalent (either ...	2020	33068733
coronavirus: proteomics analysis of chicken kidney tissue infected with variant 2 (is-1494)-like avian infectious bronchitis virus.	avian infectious bronchitis virus is one of the most important gammacoronaviruses, which causes a highly contagious disease. in this study, we investigated changes in the proteome of kidney tissue of specific-pathogen-free (spf) chickens that were infected with an isolate of the nephrotropic variant 2 genotype (is/1494/06) of avian coronavirus. twenty 1-day-old spf white leghorn chickens were randomly divided into two groups, each comprising 10 chickens, which were kept in separate positive-pres ...	2020	33083914
novel cyclovirus identified in broiler chickens with transmissible viral proventriculitis in china.	in october 2018, an outbreak of transmissible viral proventriculitis (tvp) occurred in 30-day-old commercial broiler chickens on a farm in weifang, china. tvp, an infectious viral disease characterized by runting and stunting, is associated with many viruses, and has a significant economic impact on the global poultry industry. tvp is diagnosed according to clinical symptoms, gross and histological lesions, and negative pcr results for pathogenic bacteria, avian leukosis virus subgroup j, marek' ...	2020	33134354
genetic and antigenic heterogeneity of gi-1/massachusetts lineage infectious bronchitis virus variants recently isolated in china.	four gi-1/massachusetts-type (gi-1/mass-type) infectious bronchitis virus (ibv) strains were isolated and the complete genomes of these isolates, coupled with the mass-type live-attenuated vaccine h120 and the mass-type pathogenic m41 strains, were sequenced in the present study. our results show that isolates ljl/140820 and i0306/17 may be derived from the ma5 (another mass-type live-attenuated vaccine strain) and h120 vaccine strains, respectively. the i1124/16 strain was found to be a m41 var ...	2020	33142461
the chicken mhc: insights into genetic resistance, immunity, and inflammation following infectious bronchitis virus infections.	the chicken immune system has provided an immense contribution to basic immunology knowledge by establishing major landmarks and discoveries that defined concepts widely used today. one of many special features on chickens is the presence of a compact and simple major histocompatibility complex (mhc). despite its simplicity, the chicken mhc maintains the essential counterpart genes of the mammalian mhc, allowing for a strong association to be detected between the mhc and resistance or susceptibi ...	2020	33147703
comparative trachea transcriptome analysis between avian infectious bronchitis virus and avian pathogenic e. coli individual infection and co-infection in spf chickens.	avian infectious bronchitis virus (ibv) and avian pathogenic escherichia coli (apec) are two important respiratory pathogens in the chicken. the co-infection can lead to chronic complications and considerable economic losses in the poultry industry worldwide. in the present study, we compared differential transcriptional profiles in the trachea tissue of three infected groups (ibv, apec, and co-infection) with the control group to investigate transcriptome profile changes at the early stage of t ...	2020	33151740