Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| new antimicrobial contact catalyst killing antibiotic resistant clinical and waterborne pathogens. | microbial growth on medical and technical devices is a big health issue, particularly when microorganisms aggregate to form biofilms. moreover, the occurrence of antibiotic-resistant bacteria in the clinical environment is dramatically growing, making treatment of bacterial infections very challenging. in search of an alternative, we studied a novel antimicrobial surface coating based on micro galvanic elements formed by silver and ruthenium with surface catalytic properties. the antimicrobial c ... | 2015 | 25746238 |
| molecular mechanisms of resistance to human pathogenic bacteria in caenorhabditis elegans by mev-1 mediated oxidative stress. | both mutation and knockdown in mev-1 gene render caenorhabditis elegans susceptibility to enterococcus faecalis infection. however, the mechanisms by which of mev-1 defects pathogen resistance remain unclear. here we show that mev-1rnai causes a dramatic decrease in oxidative stress and antioxidant enzyme expressions, thereby leading to increased e. faecalis accumulation in nematode intestine. mitochondrial superoxide change after infection induced these oxidative responses through daf-16 activi ... | 2015 | 25747713 |
| [analysis of pathogenic bacteria and drug resistance in neonatal purulent meningitis]. | to study the clinical characteristics, pathogenic bacteria, and antibiotics resistance of neonatal purulent meningitis in order to provide the guide for early diagnosis and appropriate treatment. | 2015 | 25748405 |
| ag-loaded mesoporous bioactive glasses against enterococcus faecalis biofilm in root canal of human teeth. | ag-loaded mesoporous bioactive glass (ag-mbg) powders were synthesized and characterized. the ions release of ag-mbgs in tris-hcl and the ph stability of simulated body fluids after immersing ag-mbgs were tested. root canals were inoculated with enterococcus faecalis for 4 weeks, and the antibacterial activity of mbgs, ag-mbgs and calcium hydroxide against e. faecalis biofilm were evaluated. results showed that ag-mbgs possessed highly ordered mesoporous structure with silver nanoparticles depos ... | 2015 | 25748459 |
| fluid dynamics and biofilm removal generated by syringe-delivered and 2 ultrasonic-assisted irrigation methods: a novel experimental approach. | thorough understanding of fluid dynamics in root canal irrigation and corresponding antibiofilm capacity will support improved disinfection strategies. this study aimed to develop a standardized, simulated root canal model that allows real-time analysis of fluid/irrigation dynamics and its correlation with biofilm elimination. | 2015 | 25749254 |
| quantitative profiling of colorectal cancer-associated bacteria reveals associations between fusobacterium spp., enterotoxigenic bacteroides fragilis (etbf) and clinicopathological features of colorectal cancer. | various studies have presented clinical or in vitro evidence linking bacteria to colorectal cancer, but these bacteria have not previously been concurrently quantified by qpcr in a single cohort. we quantify these bacteria (fusobacterium spp., streptococcus gallolyticus, enterococcus faecalis, enterotoxigenic bacteroides fragilis (etbf), enteropathogenic escherichia coli (epec), and afac- or pks-positive e. coli) in paired tumour and normal tissue samples from 55 colorectal cancer patients. we f ... | 2015 | 25751261 |
| β-lactams enhance daptomycin activity against vancomycin-resistant enterococcus faecalis and enterococcus faecium in in vitro pharmacokinetic/pharmacodynamic models. | enterococcus faecalis and enterococcus faecium are frequently resistant to vancomycin and β-lactams. in enterococcal infections with reduced glycopeptide susceptibility, combination therapy is often administered. our objective was to conduct pharmacokinetic/pharmacodynamic (pk/pd) models to evaluate β-lactam synergy with daptomycin (dap) against resistant enterococci. one e. faecalis strain (r6981) and two e. faecium strains (r6370 and 8019) were evaluated. dap mics were obtained. all strains we ... | 2015 | 25753639 |
| inoculation of tannin-degrading bacteria into novel hosts increases performance on tannin-rich diets. | it has been hypothesized that herbivores host tannin-degrading bacteria (tdb) to overcome the toxic challenges posed by plant tannins. while tdb have been isolated from the guts of numerous mammals, their functional significance to their hosts has never been explicitly tested. we introduced tdb into lab rats, which do not host tdb, and measured host performance on tannin-rich diets. we first isolated three species of tdb, escherichia coli, bacillus subtilis and enterococcus faecalis, from the gu ... | 2016 | 25753857 |
| antimicrobial action of oleanolic acid on listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. | this study investigated the antimicrobial action of oleanolic acid against listeria monocytogenes, enterococcus faecium, and enterococcus faecalis. to determine the cytotoxicity of oleanolic acid, hep-2 cells were incubated with oleanolic acid at 37°c. mics (minimal inhibition concentrations) for l. monocytogenes, e. faecium, and e. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid a ... | 2015 | 25756202 |
| fluoroquinolone resistance in bacteremic and low risk febrile neutropenic patients with cancer. | the low risk febrile neutropenic patients with multinational association for supportive care in cancer (mascc) score of more than 20 are recommended to be treated with fluoroquinolone-based oral treatment by the national comprehensive cancer network (nccn) guideline. this recommendation relies, at least partially, on the high sensitivity of the blood culture isolates to fluoroquinolone in clinical trials conducted in western countries. whether this also applies in middle or low income countries ... | 2015 | 25763661 |
| bacteriological findings and antimicrobial susceptibility pattern of isolated pathogens from visual threatening ocular infections. | to study the profiles and antimicrobial susceptibility pattern of isolated bacteria from visual threatening ocular infection. | 2015 | 25764616 |
| re: enterococcus faecalis subverts and invades the host urothelium in patients with chronic urinary tract infection. | 2015 | 25765392 | |
| could β-hemolytic, group b enterococcus faecalis be mistaken for streptococcus agalactiae? | a β-hemolytic enterococcus faecalis strain agglutinating lancefield group a, b, c, d, f, and g antisera was observed from a rectovaginal swab, in the context of antenatal screening for streptococcus agalactiae (group b streptococcus [gbs]). this is the first multi-lancefield antisera-agglutinating isolate of this species, and it raised particular concern, as it may mimic gbs, leading to false reporting and useless receipt of intrapartum antibiotics. | 2015 | 25766004 |
| ammonia sanitization of blackwater for safe use as fertilizer. | source-separated blackwater from low-flush toilets contains plant-available nutrients and can be used as a fertilizer. the aim of the study was to evaluate the impact on pathogen inactivation when treating blackwater with urea and/or lime. blackwater was spiked with salmonella typhimurium, escherichia coli o157, enterococcus faecalis, and ascaris suum eggs, and treated with urea and/or lime in concentrations up to 0.1% w/w. the bottles were kept in a storage facility (manure slurry tank) for 102 ... | 2015 | 25768229 |
| rapid fluorescence-based measurement of toxicity in anaerobic digestion. | a rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. by initially using a pure facultative anaerobic strain, enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (pcp). the technique revealed significant metabolic changes in enterococcus faecalis with a pcp spike ranging from 0.05 to 100 mg/l, and ... | 2015 | 25768985 |
| prevalence, diversity and characterization of enterococci from three coraciiform birds. | coraciiform birds hoopoe (upupa epops), common kingfisher (alcedo atthis) and european roller (coracius garrulus) were examined for enterococci in their cloacae and uropygial glands. the enterococcal isolates were identified at the species level using several genomic and proteomic methods, screened for antibiotic susceptibility and genotyped by pulsed-field gel electrophoresis (pfge). clonality of isolates from the common kingfisher was also assessed by multi-locus sequence typing (mlst). using ... | 2015 | 25772302 |
| efficacy of low concentrations of sodium hypochlorite and low-powered er,cr:ysgg laser activated irrigation against an enterococcus faecalis biofilm. | to establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without er,cr:ysgg laser activation on enterococcus faecalis biofilms in extracted teeth. | 2016 | 25772335 |
| a genomic virulence reference map of enterococcus faecalis reveals an important contribution of phage03-like elements in nosocomial genetic lineages to pathogenicity in a caenorhabditis elegans infection model. | in the present study, the commensal and pathogenic host-microbe interaction of enterococcus faecalis was explored using a caenorhabditis elegans model system. the virulence of 28 e. faecalis isolates representing 24 multilocus sequence types (mlsts), including human commensal and clinical isolates as well as isolates from animals and of insect origin, was investigated using c. elegans strain glp-4 (bn2ts); sek-1 (km4). this revealed that 6 e. faecalis isolates behaved in a commensal manner with ... | 2015 | 25776747 |
| unraveling antimicrobial resistance genes and phenotype patterns among enterococcus faecalis isolated from retail chicken products in japan. | multidrug-resistant enterococci are considered crucial drivers for the dissemination of antimicrobial resistance determinants within and beyond a genus. these organisms may pass numerous resistance determinants to other harmful pathogens, whose multiple resistances would cause adverse consequences. therefore, an understanding of the coexistence epidemiology of resistance genes is critical, but such information remains limited. in this study, our first objective was to determine the prevalence of ... | 2015 | 25781022 |
| increased membrane surface positive charge and altered membrane fluidity leads to cationic antimicrobial peptide resistance in enterococcus faecalis. | to understand the role of cell membrane phospholipids during resistance development to cationic antimicrobial peptides (camps) in enterococcus faecalis, gradual dose-dependent single exposure pediocin-resistant (pedr) mutants of e. faecalis (efv2.1, efv3.1, efv3.2, efv4.1, efv4.2, efv5.1, efv5.2 and efv5.3), conferring simultaneous resistance to other camps, selected in previous study were characterized for cell membrane phospholipid head-groups and fatty acid composition. the involvement of pho ... | 2015 | 25782727 |
| hydrogel-based microfluidic incubator for microorganism cultivation and analyses. | this work presents an array of microfluidic chambers for on-chip culturing of microorganisms in static and continuous shear-free operation modes. the unique design comprises an in-situ polymerized hydrogel that forms gas and reagent permeable culture wells in a glass chip. utilizing a hydrophilic substrate increases usability by autonomous capillary priming. the thin gel barrier enables efficient oxygen supply and facilitates on-chip analysis by chemical access through the gel without introducin ... | 2015 | 25784966 |
| antimicrobial activity of plectasin nz2114 in combination with cell wall targeting antibiotics against vana-type enterococcus faecalis. | antimicrobial peptide plectasin targeting bacterial cell wall precursor lipid ii has been reported to be active against benzylpenicillin-resistant streptococcus pneumoniae but less potent against vancomycin-resistant enterococci than their susceptible counterparts. the aim of this work was to test plectasin nz2114 in combination with cell wall targeting antibiotics on vancomycin-resistant enterococcus faecalis. the activity of antibiotic combinations was evaluated against vana-type vancomycin-re ... | 2015 | 25785733 |
| cytotoxic and antibacterial angucycline- and prodigiosin-analogues from the deep-sea derived streptomyces sp. scsio 11594. | two new c-glycoside angucyclines, marangucycline a (1) and marangucycline b (2), along with three known compounds, dehydroxyaquayamycin (3), undecylprodigiosin (4) and metacycloprodigiosin (5), have been identified as products of the deep-sea sediment strain streptomyces sp. scsio 11594. new structures were elucidated on the basis of hresims, 1d and 2d nmr analyses and comparisons to previously reported datasets. compounds 2 and 4 displayed in vitro cytotoxicity against four cancer cell lines a5 ... | 2015 | 25786061 |
| biological evaluation and molecular modelling study of thiosemicarbazide derivatives as bacterial type iia topoisomerases inhibitors. | in the present article, we describe the inhibitory potency of nine thiosemicarbazide derivatives against bacterial type iia topoisomerases, their antibacterial profile and molecular modelling evaluation. we found that one of the tested compounds, compound 7, significantly inhibits activity of staphylococcus aureus dna gyrase with an ic(50) below 15 μm. besides, this compound displays antibacterial activity on reference staphylococuss spp. and enterococcus faecalis strains as well as clinical s. ... | 2016 | 25792505 |
| antimicrobial activity of seven essential oils from iranian aromatic plants against common causes of oral infections. | over the past two decades, there has been a growing trend in using oral hygienic products originating from natural resources such as essential oils (eos) and plant extracts. seven aromatic plants used in this study are among popular traditional iranian medicinal plants with potential application in modern medicine as anti-oral infectious diseases. | 2015 | 25793100 |
| adenosine tri-phosphate (atp)-based cleaning monitoring in health care: how rapidly does environmental atp deteriorate? | ensuring cleaning compliance of housekeeping staff is critical to ensure adequate application of surface disinfectants. adenosine triphosphate (atp) testing has been recommended as a way to monitor cleaning compliance; however, little is known about the stability of atp on environmental surfaces. | 2015 | 25794441 |
| detection of vancomycin-resistant enterococcus faecalis st6-vanb2 and e. faecium st915-vana in faecal samples of wild rattus rattus in spain. | the detection of vancomycin-resistant-enterococci (vre) among wild animals represents a worrisome public health concern. the objectives of the study were to determine the possible presence of vre in faecal samples of wild small mammals in spain, to characterize the vancomycin resistance mechanisms and genetic lineages of recovered isolates and to know the diversity of enterococcal species in these animals. a total of 155 faecal samples from small mammals were inoculated in slanetz-bartley agar s ... | 2015 | 25795517 |
| antibacterial and antioxidant xanthones and benzophenone from garcinia smeathmannii. | a new prenylated xanthone, 1,3,5,8-tetrahydroxy-2-(3-methybut-2-enyl)-4-(3,7-dimethylocta-2,6-dienyl) xanthone (1), and a new benzophenone (2), together with four known xanthone derivatives, cheffouxanthone (3), smeathxanthone a (4), smeathxanthone b (5), ananixanthone (6), and two pentacyclic triterpenes, epi-friedelinol (7) and friedelin (8), were isolated from the stem bark of garcinia smeathmannii. the structures of the compounds were elucidated on the basis of 1d and 2d nmr experiments, and ... | 2015 | 25798641 |
| enterococcus species diversity and molecular characterization of biomarker genes in enterococcus faecalis in port blair bay, andaman and nicobar islands, india. | this study was performed to evaluate the abundance and diversity of enterococcus sp. and the distribution of biomarker genes in enterococcus faecalis in port blair bay, andaman and nicobar islands. the enterococcus sp. densities at the seven sampling stations were highly influenced by tidal fluctuations and season. the distributions and diversities of species varied in the inner and outer regions of port blair bay. among the 1816 total isolates, the occurrence of fecal enterococcus was high (1.7 ... | 2015 | 25798888 |
| evaluation of apically extruded bacteria associated with different nickel-titanium systems. | the aim of this in vitro study was to evaluate the amount of apically extruded bacteria associated with several root canal preparation systems. | 2015 | 25799537 |
| biodiversity and γ-aminobutyric acid production by lactic acid bacteria isolated from traditional alpine raw cow's milk cheeses. | "nostrano-cheeses" are traditional alpine cheeses made from raw cow's milk in trentino-alto adige, italy. this study identified lactic acid bacteria (lab) developing during maturation of "nostrano-cheeses" and evaluated their potential to produce γ-aminobutyric acid (gaba), an immunologically active compound and neurotransmitter. cheese samples were collected on six cheese-making days, in three dairy factories located in different areas of trentino and at different stages of cheese ripening (24 ... | 2015 | 25802859 |
| antimicrobial activity of apitoxin, melittin and phospholipase a₂ of honey bee (apis mellifera) venom against oral pathogens. | in this work, we used the minimum inhibitory concentration (mic) technique to evaluate the antibacterial potential of the apitoxin produced by apis mellifera bees against the causative agents of tooth decay. apitoxin was assayed in natura and in the commercially available form. the antibacterial actions of the main components of this apitoxin, phospholipase a2, and melittin were also assessed, alone and in combination. the following bacteria were tested: streptococcus salivarius, s. sobrinus, s. ... | 2015 | 25806982 |
| bactericidal activity of curcumin i is associated with damaging of bacterial membrane. | curcumin, an important constituent of turmeric, is known for various biological activities, primarily due to its antioxidant mechanism. the present study focused on the antibacterial activity of curcumin i, a significant component of commercial curcumin, against four genera of bacteria, including those that are gram-positive (staphylococcus aureus and enterococcus faecalis) and gram-negative (escherichia coli and pseudomonas aeruginosa). these represent prominent human pathogens, particularly in ... | 2015 | 25811596 |
| optimizing antibiotic therapy of bacteremia and endocarditis due to staphylococci and enterococci: new insights and evidence from the literature. | gram-positive cocci are a well-recognised major cause of nosocomial infection worldwide. bloodstream infections due to methicillin-resistant staphylococcus aureus, methicillin-resistant coagulase-negative staphylococci, and multi-drug resistant enterococci are a cause of concern for physicians due to their related morbidity and mortality rates. aim of this article is to review the current state of knowledge regarding the management of bsi caused by staphylococci and enterococci, including infect ... | 2015 | 25813608 |
| matrix metalloproteinase inhibitor as an antimicrobial agent to eradicate enterococcus faecalis biofilm. | successful endodontic treatment outcomes require new strategies for the complete eradication of microbial biofilms in the root canal system. matrix metalloproteinases (mmps) are essential enzymes in microbial cell growth and homeostasis, and they require transition metal ion cofactors to function. targeting mmp activity also preserves dentin collagen integrity. in this study, 1,10-phenanthroline-5,6-dione (phendione), a metal chelator, was tested as a potentially novel antimicrobial agent agains ... | 2015 | 25814242 |
| antibacterial activity of synthetic peptides derived from lactoferricin against escherichia coli atcc 25922 and enterococcus faecalis atcc 29212. | peptides derived from human and bovine lactoferricin were designed, synthesized, purified, and characterized using rp-hplc and maldi-tof-ms. specific changes in the sequences were designed as (i) the incorporation of unnatural amino acids in the sequence, the (ii) reduction or (iii) elongation of the peptide chain length, and (iv) synthesis of molecules with different number of branches containing the same sequence. for each peptide, the antibacterial activity against escherichia coli atcc 25922 ... | 2015 | 25815317 |
| bacterial contamination in tips of electrocautery devices during total hip arthroplasty. | surgical equipment can become contaminated during surgery. it is unknown if electrocautery tips can become contaminated in clean orthopedic procedures despite the produced heat. therefore, we conducted a prospective study to address this concern. the tips from 25 primary and 25 aseptic revision thas were collected and an additional 5 sterile tips served as negative controls. aerobic and anaerobic cultures were incubated for a minimum of 3 days. there were 3 positive cultures (6%); one in primary ... | 2015 | 25817186 |
| genome modification in enterococcus faecalis og1rf assessed by bisulfite sequencing and single-molecule real-time sequencing. | enterococcus faecalis is a gram-positive bacterium that natively colonizes the human gastrointestinal tract and opportunistically causes life-threatening infections. multidrug-resistant (mdr) e. faecalis strains have emerged, reducing treatment options for these infections. mdr e. faecalis strains have large genomes containing mobile genetic elements (mges) that harbor genes for antibiotic resistance and virulence determinants. bacteria commonly possess genome defense mechanisms to block mge acq ... | 2015 | 25825433 |
| comparative evaluation of antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite, and chlorhexidine against enterococcus faecalis and candida albicans. | the aim of this study is to compare the antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite (naocl), and chlorhexidine (chx) against enterococcus faecalis and candida albicans. | 2017 | 25829691 |
| evaluation of minimal inhibitory concentration of two new materials using tube dilution method: an in vitro study. | the aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials mta plus and biodentine with proroot mta using tube dilution method. | 2017 | 25829698 |
| 2-alkyl(aryl)-quinazolin-4(3h)-thiones, 2-r-(quinazolin-4(3h)-ylthio)carboxylic acids and amides: synthesis, molecular docking, antimicrobial and anticancer properties. | in this study, a series of novel 2-alkyl(aryl)-quinazolin-4(3h)-thiones, 2-r-(quinazolin-4(3h)-ylthio)carboxylic acids and amides were synthesized and evaluated for antimicrobial and anticancer activities. their structure was confirmed by elemental analysis and spectral data (ft-ir, lc-ms, (1)h-nmr). antimicrobial activity was tested in vitro against staphylococcus aureus, enterococcus faecalis, enterobacter aerogenes, pseudomonas aeruginosa, escherichia coli, klebsiella pneumonia, candida albic ... | 2016 | 25831026 |
| different extracts of zingiber officinale decrease enterococcus faecalis infection in galleria mellonella. | dried, fresh and glycolic extracts of zingiber officinale were obtained to evaluate the action against g. mellonella survival assay against enterococcus faecalis infection. eighty larvae were divided into: 1) e. faecalis suspension (control); 2) e. faecalis + fresh extract of z. officinale (feo); 3) e. faecalis + dried extract of z. officinale (deo); 4) e. faecalis + glycolic extract of z. officinale (geo); 5) phosphate buffered saline (pbs). for control group, a 5 μl inoculum of standardized su ... | 2016 | 25831098 |
| auranofin exerts broad-spectrum bactericidal activities by targeting thiol-redox homeostasis. | infections caused by antibiotic-resistant bacteria are a rising public health threat and make the identification of new antibiotics a priority. from a cell-based screen for bactericidal compounds against mycobacterium tuberculosis under nutrient-deprivation conditions we identified auranofin, an orally bioavailable fda-approved antirheumatic drug, as having potent bactericidal activities against both replicating and nonreplicating m. tuberculosis. we also found that auranofin is active against o ... | 2015 | 25831516 |
| anaerobic degradation of tannins in acacia nilotica pods by enterococcus faecalis in co-culture with ruminal microbiota. | 2015 | 25833679 | |
| prevalence, resistance patterns, and risk factors for antimicrobial resistance in bacteria from retail chicken meat in colombia. | as a step toward implementing the colombian integrated program for antimicrobial resistance surveillance (coipars), this study aimed to establish the baseline antimicrobial resistance patterns of salmonella serovars, escherichia coli, and enterococcus spp. isolates in retail poultry meat from independent stores and from a main chain distributor center. mics of the isolates were determined for antimicrobials used both in humans and animals, using an automated system. salmonella serovars were isol ... | 2015 | 25836401 |
| purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris. | malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ... | 2016 | 25837975 |
| enterococcus faecalis lipoteichoic acid suppresses aggregatibacter actinomycetemcomitans lipopolysaccharide-induced il-8 expression in human periodontal ligament cells. | periodontitis is caused by multi-bacterial infection and aggregatibacter actinomycetemcomitans and enterococcus faecalis are closely associated with inflammatory periodontal diseases. although lipopolysaccharide (lps) of a. actinomycetemcomitans (aa.lps) and lipoteichoic acid of e. faecalis (ef.lta) are considered to be major virulence factors evoking inflammatory responses, their combinatorial effect on the induction of chemokines has not been investigated. in this study, we investigated the in ... | 2015 | 25840438 |
| immune response profile against persistent endodontic pathogens candida albicans and enterococcus faecalis in vitro. | persistent microorganisms such as candida albicans and enterococcus faecalis might be directly related to endodontic treatment failure. the host response to these microorganisms impairs the reestablishment of intraradicular and periradicular health. | 2015 | 25845887 |
| nanostructured sensor based on carbon nanotubes and clavanin a for bacterial detection. | unusual methods for specific detection of pathogenic bacteria are becoming key points for control and identification of problems related to health and (bio)safety. in this context, this work aims to propose a new approach for the development of nanostructured biosensors based on carbon nanotubes (cnts) and antimicrobial peptides for bacterial detection. firstly, the antimicrobial peptide clavanin a (clava) was chemically immobilized on cnts and surface-immobilized clava was used to detect klebsi ... | 2015 | 25847459 |
| [antimicrobial activity of actinomycetale isolated from the lagoon in algeria]. | in the aim of the study of the taxonomy and the antimicrobial activity, a strain of actinomycete sm2/2gf which was isolated from sediment of the lagoon el-mellah which is situated in the city of el-kala in the northeast of algeria, was tested against diverse pathogenic microorganisms and against a gram-negative bacterium pseudomonas alcaliphila which was isolated from water of the lagoon el-mellah. the phenotypic and the molecular characteristics show that the isolate sm2/2gf belongs to the kind ... | 2015 | 25847739 |
| development and evaluation of a quadruplex taq man real-time pcr assay for simultaneous detection of clinical isolates of enterococcus faecalis, enterococcus faecium and their vana and vanb genotypes. | we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. | 2014 | 25848524 |
| urinary tract infections: epidemiology, mechanisms of infection and treatment options. | urinary tract infections (utis) are a severe public health problem and are caused by a range of pathogens, but most commonly by escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterococcus faecalis and staphylococcus saprophyticus. high recurrence rates and increasing antimicrobial resistance among uropathogens threaten to greatly increase the economic burden of these infections. in this review, we discuss how basic science studies are elucidating the molecular details of the crossta ... | 2015 | 25853778 |
| emergence of multidrug resistant enterococci at a tertiary care centre. | enterococci have assumed great clinical importance because of their increasing resistance to various antimicrobial agents. thus, knowledge about the antibiogram of these multidrug resistant isolates is of utmost importance in formulating an effective antibiotic policy to treat these infections and reducing the morbidity and mortality. aim of this study was to assess the antimicrobial resistance pattern of enterococci and determine the prevalence of multidrug resistance among them. | 2015 | 25859076 |
| the conserved modular elements of the acyl carrier proteins of lipid synthesis are only partially interchangeable. | prior work showed that expression of acyl carrier proteins (acps) of a diverse set of bacteria replaced the function of escherichia coli acp in lipid biosynthesis. however, the acpas of lactococcus lactis and enterococcus faecalis were inactive. both failed to support growth of an e. coli acpp mutant strain. this defect seemed likely because of the helix ii sequences of the two acpas, which differed markedly from those of the proteins that supported growth. to test this premise, chimeric acps we ... | 2015 | 25861991 |
| erratum for la rosa et al., in vivo assessment of growth and virulence gene expression during commensal and pathogenic lifestyles of luxabcde-tagged enterococcus faecalis strains in murine gastrointestinal and intravenous infection models. | 2015 | 25862783 | |
| evaluation of antimicrobial effectiveness and dentine mechanical properties after use of chemical and natural auxiliary irrigants. | to evaluate the effect of gse, naocl, chx and qmix as an antimicrobial agents against enterococcus faecalis and their influence on flexural and ultimate tensile strength of root canal dentine. | 2015 | 25863158 |
| semi-synthesis of new antimicrobial esters from the natural oleanolic and maslinic acids. | in this article, we report an effective procedure for the selective isolation of oleanolic acid 1 and maslinic acid 2 (3.4 and 8.5mg/g dw, respectively) from pomace olive (olea europaea l.) using an ultrasonic bath, and the synthesis of a series of new triterpenic acid esters. the compounds were characterized by their spectral data and were evaluated for their antimicrobial activity. among the compounds tested, those having sulfur and chlorine atoms were found to be antibacterial. they showed ac ... | 2015 | 25863603 |
| incidence, risk factors, microbiology of venous catheter associated bloodstream infections--a prospective study from a tertiary care hospital. | central venous catheters (cvcs) though indispensable in current medical and intensive care treatment, also puts patients at risk of catheter related infection (cri) resulting in increased morbidity and mortality. we analysed the incidence, risk factors, bacteriological profile and antimicrobial susceptibility pattern of the isolates in central venous catheter associated bloodstream infection (cvc-bsi) in the intensive care unit (icu) patients and studied the formation of biofilm in cvcs. | 2015 | 25865976 |
| in vitro evaluation of antimicrobial photodynamic therapy associated with hydroalcoholic extracts of schinopsis brasiliensis engl.: new therapeutic perspectives. | the aim of this study was to evaluate the photodynamic potential of extracts of schinopsis brasiliensis engl. on bacteria involved in several human infections. | 2015 | 25866862 |
| dissemination of enterococcus faecalis and enterococcus faecium in a ricotta processing plant and evaluation of pathogenic and antibiotic resistance profiles. | in this work, the sources of contamination by enterococcus spp. in a ricotta processing line were evaluated. the isolated strains were tested for virulence genes (gele, cyla,b, m, esp, agg, ace, efaa, vanb), expression of virulence factors (hemolysin and gelatinase), and the resistance to 10 different antibiotics. enterococcus faecium and enterococcus faecalis were subjected to discriminatory identification by intergenic spacer region (its)-polymerase chain reaction and sequencing of the its reg ... | 2015 | 25874819 |
| comparison of fluid filtration and bacterial leakage techniques for evaluation of microleakage in endodontics. | apical leakage assessment is a way to compare the efficiency of a filling material to seal the apical region of the tooth. many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results. the aim of this study was to compare the accuracy of fluid filtration and bacterial leakage techniques in the assessment of the apical sealing ability of mineral trioxide aggregate (mta) and calcium enriched mixtu ... | 2017 | 25878674 |
| antibacterial and cytotoxic activity from the extract and fractions of a marine derived bacterium from the streptomyces genus. | marine natural products are a rich source of potent, selective, and structurally novel compounds. marine bacteria are considered the most promising source of biologically active compounds which can be applied to treat a wide range of diseases. | 2015 | 25880141 |
| antimicrobial evaluation of plants used for the treatment of diarrhoea in a rural community in northern maputaland, kwazulu-natal, south africa. | zulu people living in the rural area of maputaland (kwazulu-natal, south africa) rely heavily on medicinal plants for the treatment of diarrhoea. abundant availability of medicinal plants in the study area offers low cost health care, but scientific validation is needed in order to lend credibility to the traditional use against many ailments including diarrhoeal infections. with this in mind a study was designed to test the in vitro antimicrobial efficacy of 23 plant species which are used for ... | 2015 | 25881066 |
| probiotic potential of enterococcus faecalis strains isolated from meconium. | 107 bacterial isolates with gram positive staining and negative catalase activity, presumably assumed as lactic acid bacteria, were isolated from samples of meconium of 6 donors at roubaix hospital, in the north of france. all these bacterial isolates were identified by maldi-tof mass spectrometry as enterococcus faecalis. however, only six isolates among which e. faecalis 14, e. faecalis 28, e. faecalis 90, e. faecalis 97, and e. faecalis 101 (obtained from donor 3), and e. faecalis 93 (obtaine ... | 2015 | 25883590 |
| iatrogenic aortic insufficiency following mitral valve replacement: case report and review of the literature. | we report a 28-year-old white female who suffered significant aortic insufficiency (ai) following mitral valve (mv) replacement for endocarditis. the patient had history of rheumatoid arthritis and presented to our emergency department with a 3-month history of dyspnea, orthopnea, fevers and weight loss, worsening over 2 weeks, for which she took intermittent acetaminophen. on admission, vital signs revealed blood pressure of 99/70 mm hg, heart rate of 120 beats/minute, and temperature of 98.8 ° ... | 2015 | 25883714 |
| review and phylogenetic analysis of qac genes that reduce susceptibility to quaternary ammonium compounds in staphylococcus species. | the qac genes of staphylococcus species encode multidrug efflux pumps: membrane proteins that export toxic molecules and thus increase tolerance to a variety of compounds such as disinfecting agents, including quaternary ammonium compounds (for which they are named), intercalating dyes and some antibiotics. in stapylococcus species, six different plasmid-encoded qac efflux pumps have been described, and they belong to two major protein families. qaca and qacb are members of the major facilitator ... | 2015 | 25883793 |
| genetic relatedness and risk factor analysis of ampicillin-resistant and high-level gentamicin-resistant enterococci causing bloodstream infections in tanzanian children. | while enterococci resistant to multiple antimicrobials are spreading in hospitals worldwide, causing urinary tract, wound and bloodstream infections, there is little published data on these infections from africa. | 2015 | 25884316 |
| investigating the mobilome in clinically important lineages of enterococcus faecium and enterococcus faecalis. | the success of enterococcus faecium and e. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes encoded by mobile genetic elements (mges). here, we investigate this mobilome in successful hospital associated genetic lineages, e. faecium sequence type (st)17 (n=10) and st78 (n=10), e. faecalis st6 (n=10) and st40 (n=10) by dna microarray analyses. | 2015 | 25885771 |
| comprehensive molecular, genomic and phenotypic analysis of a major clone of enterococcus faecalis mlst st40. | enterococcus faecalis is a multifaceted microorganism known to act as a beneficial intestinal commensal bacterium. it is also a dreaded nosocomial pathogen causing life-threatening infections in hospitalised patients. isolates of a distinct mlst type st40 represent the most frequent strain type of this species, distributed worldwide and originating from various sources (animal, human, environmental) and different conditions (colonisation/infection). since enterococci are known to be highly recom ... | 2015 | 25887115 |
| evaluation of residual antibacterial effect of human radicular dentin treated with triple and double antibiotic pastes. | the aim of this study was to investigate the residual antibacterial effect of human radicular dentin treated with various concentrations of triple antibiotic paste and double antibiotic paste (dap). | 2015 | 25887806 |
| micropan: an r-package for microbial pan-genomics. | a pan-genome is defined as the set of all unique gene families found in one or more strains of a prokaryotic species. due to the extensive within-species diversity in the microbial world, the pan-genome is often many times larger than a single genome. studies of pan-genomes have become popular due to the easy access to whole-genome sequence data for prokaryotes. a pan-genome study reveals species diversity and gene families that may be of special interest, e.g because of their role in bacterial ... | 2015 | 25888166 |
| evaluation of regional antibiograms to monitor antimicrobial resistance in hampton roads, virginia. | we studied recent antibiograms (2010 to 2011) from 12 hospitals in the hampton roads area, virginia, that refer patients to a tertiary-care facility affiliated with eastern virginia medical school. the data was compiled into a regional antibiogram, and sensitivity rates of common isolates from the tertiary-care facility (central) were compared to those of referring hospitals grouped by locale. staphylococcus aureus was the most common gram- positive and e. coli the most common gram- negative org ... | 2015 | 25890362 |
| minimizing concentration of sodium hypochlorite in root canal irrigation by combination of ultrasonic irrigation with photodynamic treatment. | concentration of sodium hypochlorite (naocl) is positively correlated with its effectiveness in root canal disinfection but negatively correlated with its biocompatibility. the objective of this in vitro study was to compare the bactericidal effects among ultrasonic irrigation with different concentration of naocl alone or together with photodynamic treatment (pdt) against enterococcus faecalis (e. faecalis) in infected root canals. one hundred and twenty bovine root canals contaminated with e. ... | 2015 | 25892274 |
| in vitro antimicrobial and anti-endotoxin action of zingiber officinale as auxiliary chemical and medicament combined to calcium hydroxide and chlorhexidine. | this study was conducted in vitro to compare the effectiveness of zingiber officinale as an auxiliary chemical substance followed by placement of different intra-canal medication in removing endotoxins and cultivable micro-organisms from infected root canals. | 2015 | 25892281 |
| total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis. | most studies of the microbiota in root canal-treated teeth focused only on the main canal, not distinguishing regions nor incorporating the intricate anatomy in the analysis. moreover, most of them provided only prevalence data. this study was designed to evaluate the total bacterial counts and the presence, levels, and relative abundance of candidate endodontic pathogens exclusively in the apical root canal system associated with post-treatment apical periodontitis. | 2015 | 25892512 |
| urinary tract infections and antimicrobial sensitivity among diabetic patients at khartoum, sudan. | patients with diabetes mellitus (dm) are more susceptible to urinary tract infection (uti) than non-diabetics. due to the emergence of multidrug resistant (mdr) uropathogenic strains, the choice of antimicrobial agent is restricted. this study investigated the epidemiology of uti, antimicrobial susceptibility, and resistance patterns of bacterial isolates from adult diabetic patients. | 2015 | 25896611 |
| a variable dna recognition site organization establishes the liar-mediated cell envelope stress response of enterococci to daptomycin. | liar is a 'master regulator' of the cell envelope stress response in enterococci and many other gram-positive organisms. mutations to liar can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. liar is phosphorylated in response to membrane stress to regulate downstream target operons. using dna footprinting of the regions upstream of the liaxyz and liafsr operons we show that liar binds an extended stretch of dna that extends beyond the propos ... | 2015 | 25897118 |
| quantitatively predicting bacterial adhesion using surface free energy determined with a spectrophotometric method. | bacterial adhesion onto solid surfaces is of importance in a wide spectrum of problems, including environmental microbiology, biomedical research, and various industrial applications. despite many research efforts, present thermodynamic models that rely on the evaluation of the adhesion energy are often elusive in predicting the bacterial adhesion behavior. here, we developed a new spectrophotometric method to determine the surface free energy (sfe) of bacterial cells. the adhesion behaviors of ... | 2015 | 25898026 |
| competition between vanu(g) repressor and vanr(g) activator leads to rheostatic control of vang vancomycin resistance operon expression. | enterococcus faecalis bm4518 is resistant to vancomycin by synthesis of peptidoglycan precursors ending in d-alanyl-d-serine. in the chromosomal vang locus, transcription of the resistance genes from the pyg resistance promoter is inducible and, upstream from these genes, there is an unusual three-component regulatory system encoded by the vanurs(g) operon from the p(ug) regulatory promoter. in contrast to the other van operons in enterococci, the vang operon possesses the additional vanu(g) gen ... | 2015 | 25898178 |
| genotypic and phenotypic diversity in enterococcus faecalis: is agar invasion a pathogenicity score? | the main objective of the present study is to analyze different genotypic and phenotypic traits related to virulence in enterococcus faecalis, as well as evaluated the agar invasion phenotype in a collection of isolates with different clinical origins. | 2015 | 25904518 |
| ph-induced change in cell susceptibility to butanol in a high butanol-tolerant bacterium, enterococcus faecalis strain cm4a. | though butanol is considered as a potential biofuel, its toxicity toward microorganisms is the main bottleneck for the biological butanol production. recently, butanol-tolerant bacteria have been proposed as alternative butanol production hosts overcoming the end product inhibition. one remaining key issue to be addressed is how physicochemical properties such as ph and temperature affect microbial butanol tolerance during cultivation and fermentation. | 2015 | 25904984 |
| interplay between copper and zinc homeostasis through the transcriptional regulator zur in enterococcus faecalis. | by integrating the microarray expression data and a global e. faecalis transcriptional network we identified a sub-network activated by zinc and copper. our analyses indicated that the transcriptional response of the bacterium to copper and zinc exposure involved the activation of two modules, module i that contains genes implicated in zinc homeostasis, including the zur transcriptional repressor, and module ii containing a set of genes associated with general stress response and basal metabolis ... | 2015 | 25906431 |
| preparation, characterization and antibacterial properties of zno/kaoline nanocomposites. | this paper describes laboratory preparation, characterization and antibacterial activity testing of zno/kaoline composites. zno/kaoline composites with 50 wt.% of zno were laboratory prepared, dried at 105 °c and calcined at 500 °c. xrpd analysis revealed that thermal treatment caused the phase transformation of zn containing precursor into zno. scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. a standard microdilution ... | 2015 | 25909474 |
| localization of the dual oxidase bli-3 and characterization of its nadph oxidase domain during infection of caenorhabditis elegans. | dual oxidases (duox) are enzymes that contain an nadph oxidase domain that produces hydrogen peroxide (h2o2) and a peroxidase domain that can utilize h2o2 to carry out a variety of reactions. the model organism caenorhabditis elegans produces the duox, bli-3, which has roles in both cuticle development and in protection against infection. in previous work, we demonstrated that while certain peroxidases were protective against the human bacterial pathogen enterococcus faecalis, the peroxidase dom ... | 2015 | 25909649 |
| a novel method for screening of potential probiotics for high adhesion capability. | to screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. lactobacillus plantarum (hm218749), lactobacillus reuteri (eu547310), and enterococcus faecalis (hm218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37°c in an anaerobic environment and could r ... | 2015 | 25912863 |
| tyrosine decarboxylase activity of enterococci grown in media with different nutritional potential: tyramine and 2-phenylethylamine accumulation and tyrdc gene expression. | the ability to accumulate tyramine and 2-phenylethylamine by two strains of enterococcus faecalis and two strains enterococcus faecium was evaluated in two cultural media added or not with tyrosine. all the enterococcal strains possessed a tyrosine decarboxylase (tyrdc) which determined tyramine accumulation in all the conditions tested, independently on the addition of high concentration of free tyrosine. enterococci differed in rate and level of biogenic amines accumulation. e. faecalis ef37 a ... | 2015 | 25914676 |
| proteomic investigation of the response of enterococcus faecalis v583 when cultivated in urine. | enterococcus faecalis is a robust bacterium, which is able to survive in and adapt to hostile environments such as the urinary tract and bladder. in this label-free quantitative proteomic study based on maxquant lfq algorithms, we identified 127 proteins present in the secretome of the clinical vancomycin-resistant isolate e. faecalis v583 and we compared proteins secreted in the initial phase of cultivation in urine with the secretome during cultivation in standard laboratory medium, 2xyt. of t ... | 2015 | 25915650 |
| enterococcus faecalis gelatinase mediates intestinal permeability via protease-activated receptor 2. | microbial protease-mediated disruption of the intestinal epithelium is a potential mechanism whereby a dysbiotic enteric microbiota can lead to disease. this mechanism was investigated using the colitogenic, protease-secreting enteric microbe enterococcus faecalis. caco-2 and t-84 epithelial cell monolayers and the mouse colonic epithelium were exposed to concentrated conditioned media (ccm) from e. faecalis v583 and e. faecalis lacking the gelatinase gene (gele). the flux of fluorescein isothio ... | 2015 | 25916983 |
| effects of photodynamic therapy on enterococcus faecalis biofilms. | microbial biofilms are involved in almost all infectious pathologies of the oral cavity. this has led to the search for novel therapies specifically aimed at biofilm elimination. in this study, we used atomic force microscopy (afm) to visualize injuries and to determine surface roughness, as well as confocal laser scanning microscopy (clsm) to enumerate live and dead bacterial cells, to determine the effects of photodynamic therapy (pdt) on enterococcus faecalis biofilms. the afm images showed t ... | 2015 | 25917515 |
| multiple roles for enterococcus faecalis glycosyltransferases in biofilm-associated antibiotic resistance, cell envelope integrity, and conjugative transfer. | the emergence of multidrug-resistant bacteria and the limited availability of new antibiotics are of increasing clinical concern. a compounding factor is the ability of microorganisms to form biofilms (communities of cells encased in a protective extracellular matrix) that are intrinsically resistant to antibiotics. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and also has the propensity to acquire resistance determinants via horizontal gene transfer. there is i ... | 2015 | 25918141 |
| genotypic variations of virulent genes in enterococcus faecium and enterococcus faecalis isolated from three hospitals in malaysia. | the genus enterococcus is of increasing significance as a cause of nosocomial infections, and this trend is exacerbated by the development of antibiotic resistance. | 2015 | 25923096 |
| effect of apically separated rotary instruments on bacterial leakage of obturated root canals using resilon. | the purpose of this study was to determine the effect of a separated rotary instrument on the time required for bacterial penetration of obturated root canals using resilon. eighty-four extracted human maxillary premolars with single canals were used in this study. group 1 consisted of teeth obturated with gutta-percha and ah-26 sealer (n=15); group 2 consisted of teeth obturated similarly to group 1 but without a separated file; group 3 consisted of teeth obturated with resilon and epiphany sea ... | 2015 | 25928974 |
| the intestinal microbiota influences campylobacter jejuni colonization and extraintestinal dissemination in mice. | campylobacter jejuni is a leading cause of human foodborne gastroenteritis worldwide. the interactions between this pathogen and the intestinal microbiome within a host are of interest as endogenous intestinal microbiota mediates a form of resistance to the pathogen. this resistance, termed colonization resistance, is the ability of commensal microbiota to prevent colonization by exogenous pathogens or opportunistic commensals. although mice normally demonstrate colonization resistance to c. jej ... | 2015 | 25934624 |
| the all-alpha domains of coupling proteins from the agrobacterium tumefaciens virb/vird4 and enterococcus faecalis pcf10-encoded type iv secretion systems confer specificity to binding of cognate dna substrates. | bacterial type iv coupling proteins (t4cps) bind and mediate the delivery of dna substrates through associated type iv secretion systems (t4sss). t4cps consist of a transmembrane domain, a conserved nucleotide-binding domain (nbd), and a sequence-variable helical bundle called the all-alpha domain (aad). in the t4cp structural prototype, plasmid r388-encoded trwb, the nbd assembles as a homohexamer resembling reca and dna ring helicases, and the aad, which sits at the channel entrance of the hom ... | 2015 | 25939830 |
| mutations associated with reduced surotomycin susceptibility in clostridium difficile and enterococcus species. | clostridium difficile infection (cdi) is an urgent public health concern causing considerable clinical and economic burdens. cdi can be treated with antibiotics, but recurrence of the disease following successful treatment of the initial episode often occurs. surotomycin is a rapidly bactericidal cyclic lipopeptide antibiotic that is in clinical trials for cdi treatment and that has demonstrated superiority over vancomycin in preventing cdi relapse. surotomycin is a structural analogue of the me ... | 2015 | 25941217 |
| antimicrobial 2-hydroxyisocaproic acid and chlorhexidine resist inactivation by dentine. | to compare the antibacterial activity of 2-hydroxyisocaproic acid (hica) with currently used root canal medicaments and to examine their interactions with potential inhibitors in nutrient-deficient and nutrient-rich conditions. | 2016 | 25944560 |
| antibacterial and antiproliferative activities of plumericin, an iridoid isolated from momordica charantia vine. | plumericin, an iridoid lactone, was isolated with relatively high yield from momordica charantia vine using the supercritical fluid extraction (sfe) and the separation box (sepbox) comprising dual combination of high-performance liquid chromatography and solid phase extraction. this compound showed antibacterial activity against enterococcus faecalis and bacillus subtilis with minimum inhibitory concentration (mic) values better than cloxacillin. plumericin potently inhibited proliferation of tw ... | 2015 | 25945113 |
| collagen degradation and mmp9 activation by enterococcus faecalis contribute to intestinal anastomotic leak. | even under the most expert care, a properly constructed intestinal anastomosis can fail to heal, resulting in leakage of its contents, peritonitis, and sepsis. the cause of anastomotic leak remains unknown, and its incidence has not changed in decades. we demonstrate that the commensal bacterium enterococcus faecalis contributes to the pathogenesis of anastomotic leak through its capacity to degrade collagen and to activate tissue matrix metalloproteinase 9 (mmp9) in host intestinal tissues. we ... | 2015 | 25947163 |
| the origin of endodontic enterococcus faecalis explored by comparison of virulence factor patterns and antibiotic resistance to that of isolates from stool samples, blood cultures and food. | to elucidate the origin of enterococcus faecalis isolated from secondary root canal infections and the possibility for a foodborne transmission by comparing them to strains recovered from food, blood and stool regarding putative virulence factors and antibiotic susceptibility profiles, where strains from common origin were hypothesized to harbour similar characteristics. | 2016 | 25950381 |
| effect of nacl treatments on tyramine biosynthesis of enterococcus faecalis. | the effect of nacl stress (0 to 8%, wt/vol) on the growth and tyramine production in two enterococcus faecalis strains was examined during culture time. the growth of e. faecalis was inhibited by the increase in nacl concentration, but tyramine production was unaffected. tyramine accumulated rapidly during the logarithmic phase of the strains, and the final tyramine levels were approximately 800 μg/ml. relative gene expression of four genes in the tyrosine decarboxylase locus, namely, tyrrs, tyr ... | 2015 | 25951388 |