Publications

costs and benefits of vertical and horizontal transmission of dengue virus.	parasites can be transmitted vertically and/or horizontally, but the costs or benefits for the host of infection have only been tested after horizontal transmission. here, we report for the first time, to our knowledge, the survival, reproduction and infection of aedes aegypti during vertical and horizontal transmission of dengue virus 2 (denv-2). females infected horizontally produced more eggs, with a sex ratio skewed towards males, compared with uninfected controls. however, there was no sign ...	2016	27618859
an improved mosquito electrocuting trap that safely reproduces epidemiologically relevant metrics of mosquito human-feeding behaviours as determined by human landing catch.	reliable quantification of mosquito host-seeking behaviours is required to determine the efficacy of vector control methods. for malaria, the gold standard approach remains the risky human landing catch (hlc). here compare the performance of an improved prototype of the mosquito electrocuting grid trap (met) as a safer alternative with hlc for measuring malaria vector behaviour in dar es salaam, tanzania.	2016	27618941
predicting arboviral disease emergence using bayesian networks: a case study of dengue virus in western australia.	a bayesian belief network (bbn) for assessing the potential risk of dengue virus emergence and distribution in western australia (wa) is presented and used to identify possible hotspots of dengue outbreaks in summer and winter. the model assesses the probabilities of two kinds of events which must take place before an outbreak can occur: (1) introduction of the virus and mosquito vectors to places where human population densities are high; and (2) vector population growth rates as influenced by ...	2017	27620510
developmental expression of ecdysone-related genes associated with metamorphic changes during midgut remodeling of silkworm bombyx mori (lepidoptera:bombycidae).	steroid hormone 20-hydroxyecdysone is known as the systemic regulators of insect cells; however, how to impact the fate and function of mature and stem cells is unclear. for the first time, we report ecdysone regulatory cascades in both mature midgut cell and stem cell fractions related to developmental events by using histological, immunohistochemical, biochemical and gene expression analysis methods. ecdysone receptor-b1 (ecr-b1) and ultraspiracle 1 (usp-1) mrnas were detected mainly in mature ...	2016	27620558
[zika: a defeat in the struggle against aedes aegypti].		2016	27622433
aedes aegypti in rural areas: public health implications.		2016	27622476
presence of aedes (stegomyia) aegypti (linnaeus, 1762) and its natural infection with dengue virus at unrecorded heights in colombia.	aedes aegypti is the main vector of urban yellow fever, dengue, chikungunya and zika viruses. the biogeographical distribution of this species has expanded due to global warming, and socioeconomic and cultural factors. the changes in the altitudinal distribution patterns of this vector and its natural infection are priority fields of research to develop entomological, virological and public health surveillance strategies.	2016	27622492
agricultural chemicals: life changer for mosquito vectors in agricultural landscapes?	although many mosquito species develop within agricultural landscapes where they are potentially exposed to agricultural chemicals (fertilizers and pesticides), the effects of these chemicals on mosquito biology remain poorly understood. this study investigated the effects of sublethal concentrations of four agricultural chemicals on the life history traits of anopheles arabiensis and culex quinquefasciatus mosquitoes.	2016	27624456
small rna regulators of plant-hemipteran interactions: micromanagers with versatile roles.	non-coding small rnas (srnas) in plants have important roles in regulating biological processes, including development, reproduction, and stress responses. recent research indicates significant roles for srna-mediated gene silencing during plant-hemipteran interactions that involve all three of these biological processes. plant responses to hemipteran feeding are determined by changes in the host transcriptome that appear to be fine-tuned by srnas. the role of srna in plant defense responses is ...	2016	27625654
detection of invasive mosquito vectors using environmental dna (edna) from water samples.	repeated introductions and spread of invasive mosquito species (ims) have been recorded on a large scale these last decades worldwide. in this context, members of the mosquito genus aedes can present serious risks to public health as they have or may develop vector competence for various viral diseases. while the tiger mosquito (aedes albopictus) is a well-known vector for e.g. dengue and chikungunya viruses, the asian bush mosquito (ae. j. japonicus) and ae. koreicus have shown vector competenc ...	2016	27626642
bacterial larvicide, bacillus thuringiensis israelensis strain am 65-52 water dispersible granule formulation impacts both dengue vector, aedes aegypti (l.) population density and disease transmission in cambodia.	a multi-phased study was conducted in cambodia from 2005-2011 to measure the impact of larviciding with the bacterial larvicide, bacillus thuringiensis israelensis (bti), a water dispersible granule (wg) formulation on the vector, aedes aegypti (l.) and the epidemiology. in our studies, all in-use containers were treated at 8 g/1000 l, including smaller containers and animal feeders which were found to contribute 23% of ae aegypti pupae. the treated waters were subjected to routine water exchang ...	2016	27627758
maintenance of host dna integrity in field-preserved mosquito (diptera: culicidae) blood meals for identification by dna barcoding.	determination of the interactions between hematophagous arthropods and their hosts is a necessary component to understanding the transmission dynamics of arthropod-vectored pathogens. current molecular methods to identify hosts of blood-fed arthropods require the preservation of host dna to serve as an amplification template. during transportation to the laboratory and storage prior to molecular analysis, genetic samples need to be protected from nucleases, and the degradation effects of hydroly ...	2016	27629021
morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus.	candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ...	2016	27630042
artemisia absinthium-borne compounds as novel larvicides: effectiveness against six mosquito vectors and acute toxicity on non-target aquatic organisms.	the eco-friendly control of mosquito vectors is a crucial challenge of public health importance. here we evaluated the larvicidal potential of artemisia absinthium essential oil (eo) and its three major chemical constituents against six mosquito vectors: anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. the eo was obtained by leaf hydro-distillation. its chemical composition was analyzed using gas chromatography-mass s ...	2016	27630101
the genetic basis of host preference and resting behavior in the major african malaria vector, anopheles arabiensis.	malaria transmission is dependent on the propensity of anopheles mosquitoes to bite humans (anthropophily) instead of other dead end hosts. recent increases in the usage of long lasting insecticide treated nets (llins) in africa have been associated with reductions in highly anthropophilic and endophilic vectors such as anopheles gambiae s.s., leaving species with a broader host range, such as anopheles arabiensis, as the most prominent remaining source of transmission in many settings. an. arab ...	2016	27631375
zika virus disease cases - 50 states and the district of columbia, january 1-july 31, 2016.	zika virus is a mosquito-borne flavivirus primarily transmitted to humans by aedes aegypti mosquitoes (1). zika virus infections have also been documented through intrauterine transmission resulting in congenital infection; intrapartum transmission from a viremic mother to her newborn; sexual transmission; blood transfusion; and laboratory exposure (1-5). most zika virus infections are asymptomatic (1,6). clinical illness, when it occurs, is generally mild and characterized by acute onset of fev ...	2016	27631604
aedes aegypti d7 saliva protein inhibits dengue virus infection.	aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (denv) types 1-4. ae. aegypti transmits denv by inoculating virus-infected saliva into host skin during probing and feeding. ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. previously, we showed that ae. aegypti salivary gland extracts (sges) enhanced dissemination of denv to draining lymph nodes. in con ...	2016	27632170
analysis of genotype diversity and evolution of dengue virus serotype 2 using complete genomes.	dengue is one of the most common arboviral diseases prevalent worldwide and is caused by dengue viruses (genus flavivirus, family flaviviridae). there are four serotypes of dengue virus (denv-1 to denv-4), each of which is further subdivided into distinct genotypes. denv-2 is frequently associated with severe dengue infections and epidemics. denv-2 consists of six genotypes such as asian/american, asian i, asian ii, cosmopolitan, american and sylvatic. comparative genomic study was carried out t ...	2016	27635316
smshb, the sh2-containing adaptor protein b of schistosoma mansoni regulates venus kinase receptor signaling pathways.	venus kinase receptors (vkrs) are invertebrate receptor tyrosine kinases (rtks) formed by an extracellular venus fly trap (vft) ligand binding domain associated via a transmembrane domain with an intracellular tyrosine kinase (tk) domain. schistosoma mansoni vkrs, smvkr1 and smvkr2, are both implicated in reproductive activities of the parasite. in this work, we show that the sh2 domain-containing protein smshb is a partner of the phosphorylated form of smvkr1. expression of these proteins in xe ...	2016	27636711
lbd1 of vitellogenin receptor specifically binds to the female-specific storage protein sp1 via lbr1 and lbr3.	storage proteins are the major protein synthesized in the fat body, released into hemolymph and re-sequestered into the fat body before pupation in most insect species. storage proteins are important amino acid and nutrition resources during the non-feeding pupal period and play essential roles for the metamorphosis and oogenesis of insects. the sequestration of storage protein is a selective, specific receptor-mediated process. however, to date, the potential receptor mediating the sequestratio ...	2016	27637099
non-mendelian dominant maternal effects caused by crispr/cas9 transgenic components in drosophila melanogaster.	the crispr/cas9 system has revolutionized genomic editing. the cas9 endonuclease targets genomic dna via an experimentally determined guide rna (grna). this results in a double-strand break at the target site. we generated transgenic drosophila melanogaster in which the crispr/cas9 system was used to target a gal4 transgene in vivo to our surprise, progeny whose genomes did not contain crispr/cas9 components were still capable of mutating gal4 sequences. we demonstrate this effect was caused by ...	2016	27638686
antimosquito property of petroselinum crispum (umbellifereae) against the pyrethroid resistant and susceptible strains of aedes aegypti (diptera: culicidae).	the increasing and widespread resistance to conventional synthetic insecticides in vector populations has underscored the urgent need to establish alternatives in the mosquito management system. this study was carried out with the aim to investigate the antimosquito property, larvicidal and adulticidal potential, of plant products against both the pyrethroid-susceptible and resistant strains of aedes aegypti. seventeen plant products, including essential oils and ethanolic extracts, were obtaine ...	2016	27638800
raw sewage as breeding site to aedes (stegomyia) aegypti (diptera, culicidae).	the selection of oviposition sites by females of aedes (stegomyia) aegypti is a key factor for the larval survival and egg dispersion and has a direct influence in vector control programs. in this study, we evaluated the aspects of reproductive physiology of ae. aegypti mosquitoes tested in the presence of raw sewage. ae. aegypti females were used in oviposition bioassays according to two methodologies: (i) choice assay, in which three oviposition substrates were offered in the same cage: treatm ...	2016	27640323
salinity responsive aquaporins in the anal papillae of the larval mosquito, aedes aegypti.	the larvae of the mosquito, aedes aegypti normally inhabit freshwater (fw) where they face dilution of body fluids by osmotic influx of water. in response, the physiological actions of the anal papillae result in ion uptake while the malpighian tubules and rectum work in concert to excrete excess water. in an apparent paradox, the anal papillae express aquaporins (aqps) and are sites of water permeability which, if aqps are expressed by the epithelium, apparently exaggerates the influx of water ...	2017	27642095
another pandemic disaster looms: yellow fever spreading from angola.		2016	27642446
assessment of local mosquito species incriminates aedes aegypti as the potential vector of zika virus in australia.	within the last 10 years zika virus (zikv) has caused unprecedented epidemics of human disease in the nations and territories of the western pacific and south america, and continues to escalate in both endemic and non-endemic regions. we evaluated the vector competence of australian mosquitoes for zikv to assess their potential role in virus transmission.	2016	27643685
functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts.	as both larvae and adults, mosquitoes encounter a barrage of immune insults, ranging from microbe-rich communities in larval habitats to ingested blood-borne pathogens in adult blood meals. given that mosquito adults have evolved an efficient means of eliminating infections in their hemocoel (body cavity) via the coordinated action of their immune and circulatory systems, the goal of the present study was to determine whether such functional integration is also present in larvae.	2016	27643786
sit 2.0: 21(st) century genetic technology for the screwworm sterile-insect program.	release of sterile insects, the sterile insect technique (sit), can be an extremely effective and precise method of pest control. a study in bmc biology from the new world screwworm sit program and others shows that modern genetic methods can provide major improvements even to this well-established and highly successful sit program.see research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-016-0296-8.	2016	27643991
identification of immunity-related genes in dialeurodes citri against entomopathogenic fungus lecanicillium attenuatum by rna-seq analysis.	dialeurodes citri is a major pest in citrus producing areas, and large-scale outbreaks have occurred increasingly often in recent years. lecanicillium attenuatum is an important entomopathogenic fungus that can parasitize and kill d. citri. we separated the fungus from corpses of d. citri larvae. however, the sound immune defense system of pests makes infection by an entomopathogenic fungus difficult. here we used rna sequencing technology (rna-seq) to build a transcriptome database for d. citri ...	2016	27644092
guy1 confers complete female lethality and is a strong candidate for a male-determining factor in anopheles stephensi.	despite their importance in sexual differentiation and reproduction, y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. here, we show that guy1, a unique y chromosome gene of a major urban malaria mosquito anopheles stephensi, confers 100% female lethality when placed on the autosomes. we show that the small guy1 protein (56 amino acids in length) causes female lethality and that males carrying the transgene are reproductively more com ...	2016	27644420
zika virus: epidemiology, current phobia and preparedness for upcoming mass gatherings, with examples from world olympics and pilgrimage.	to describe zika virus (zikv) epidemiology, current phobia, and the required preparedness for its prevention during the upcoming mass gathering (mg) events.	0	27648063
dengueme: a tool for the modeling and simulation of dengue spatiotemporal dynamics.	the prevention and control of dengue are great public health challenges for many countries, particularly since 2015, as other arboviruses have been observed to interact significantly with dengue virus. different approaches and methodologies have been proposed and discussed by the research community. an important tool widely used is modeling and simulation, which help us to understand epidemic dynamics and create scenarios to support planning and decision making processes. with this aim, we propo ...	2016	27649226
origin of the zika virus revealed: a historical journey across the world.	zika virus (zikv) is an arbovirus within the flaviviridae family, the recent spread of which has promoted public concern.	2016	27650823
evaluation of clove oil, icaridin, and transfluthrin for spatial repellent effects in three tests systems against the aedes aegypti (diptera: culicidae).	one essential oil (clove oil), one skin repellent (icaridin), and one insecticide (transfluthrin) were tested for spatial repellent effects against non-blood-fed female aedes aegypti (l.) mosquitoes. the compounds were tested in acetone dilution series using a y-olfactometer, a double cage system, and a double room system. all compounds exhibited spatial repellent effects at certain concentrations. clove oil required relative high dosages to cause high effects (y-olfactometer 6 mg, double cage 6 ...	2016	27651437
rhodopsin 7-the unusual rhodopsin in drosophila.	rhodopsins are the major photopigments in the fruit fly drosophila melanogaster. drosophila express six well-characterized rhodopsins (rh1-rh6) with distinct absorption maxima and expression pattern. in 2000, when the drosophila genome was published, a novel rhodopsin gene was discovered: rhodopsin 7 (rh7). rh7 is highly conserved among the drosophila genus and is also found in other arthropods. phylogenetic trees based on protein sequences suggest that the seven drosophila rhodopsins cluster in ...	2016	27651995
the mysterious zika virus: adding to the tropical flavivirus mayhem.	until now, known as the demure cousin of dengue virus (denv) inhabiting africa, zika virus (zikv) has reinvented itself to cause explosive epidemics captivating the western hemisphere. the outbreak causing potential for zikv was realized when it made its way from africa to yap island micronesia in 2007, and in french polynesia in 2013. from there, it moved on to brazil in 2015. now zikv has infected people in more than 33 countries in central and south america and the caribbean. moreover the epi ...	2016	27652985
dna microarray platform for detection and surveillance of viruses transmitted by small mammals and arthropods.	viruses transmitted by small mammals and arthropods serve as global threats to humans. most emergent and re-emergent viral agents are transmitted by these groups; therefore, the development of high-throughput screening methods for the detection and surveillance of such viruses is of great interest. in this study, we describe a dna microarray platform that can be used for screening all viruses transmitted by small mammals and arthropods (smaviruschip) with nucleotide sequences that have been depo ...	2016	27654806
vector competence of french polynesian aedes aegypti and aedes polynesiensis for zika virus.	in 2013-2014, french polynesia experienced for the first time a zika outbreak. two aedes mosquitoes may have contributed to zika virus (zikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito.	2016	27654962
the tomato spotted wilt virus genome is processed differentially in its plant host arachis hypogaea and its thrips vector frankliniella fusca.	thrips-transmitted tospoviruses are economically important viruses affecting a wide range of field and horticultural crops worldwide. tomato spotted wilt virus (tswv) is the type member of the tospovirus genus with a broad host range of more than 900 plant species. interactions between these viruses and their plant hosts and insect vectors via rnai pathways are likely a key determinant of pathogenicity. the current investigation, for the first time, compares biogenesis of small rnas between the ...	2016	27656190
dengue protease substrate recognition: binding of the prime side.	dengue virus (denv), transmitted predominantly in tropical and subtropical regions by the mosquito aedes aegypti, infects millions of people and leads to dengue fever and thousands of deaths each year. there are no direct-acting antivirals to combat denv, and molecular and structural knowledge is required to develop such compounds. the dengue ns2b/ns3 protease is a promising target for direct-acting antivirals, as viral polyprotein cleavage during replication is required for the maturation of th ...	2016	27657335
the whole genome sequence of the mediterranean fruit fly, ceratitis capitata (wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species.	the mediterranean fruit fly (medfly), ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. it exhibits a unique ability to invade and adapt to ecological niches throughout tropical and subtropical regions of the world, though medfly infestations have been prevented and controlled by the sterile insect technique (sit) as part of integrated pest management programs (ipms). the genetic analysis and manipulation of medf ...	2016	27659211
predicted structure of a minus-c obp from batocera horsfieldi (hope) suggests an intermediate structure in evolution of obps.	odorant binding proteins (obps) transport hydrophobic odorants from the environment to odorant receptors and play an important role in specific recognition of volatiles. here, we expressed and purified a minus-c obp, bhorobpm2, from batocera horsfieldi, a major pest of popolus, to determine its binding characteristics with 58 candidate volatiles using a fluorescence competition-binding assay. we showed that bhorobpm2 exhibited high binding affinity with chain volatiles and that ligands were sele ...	2016	27659921
potential of camellia sinensis proanthocyanidins-rich fraction for controlling malaria mosquito populations through disruption of larval development.	anopheles arabiensis and a. gambiae (sensu stricto) are the most prolific afrotropical malaria vectors. population control efforts of these two vectors have been hampered by extremely diverse larval breeding sites and widespread resistance to currently available insecticides. control of mosquito larval stages using bioactive compounds of plant origin has the potential to suppress vector populations leading to concomitant reduction in disease transmission rates. in this study, we evaluated the ef ...	2016	27660043
dengue serotype immune-interactions and their consequences for vaccine impact predictions.	dengue is one of the most important and wide-spread viral infections affecting human populations. the last few decades have seen a dramatic increase in the global burden of dengue, with the virus now being endemic or near-endemic in over 100 countries world-wide. a recombinant tetravalent vaccine candidate (cyd-tdv) has recently completed phase iii clinical efficacy trials in south east asia and latin america and has been licensed for use in several countries. the trial results showed moderate-t ...	2016	27663790
arbovirosis and potential transmission blocking vaccines.	infectious diseases caused by arboviruses (viruses transmitted by arthropods) are undergoing unprecedented epidemic activity and geographic expansion. with the recent introduction of west nile virus (1999), chikungunya virus (2013) and zika virus (2015) to the americas, stopping or even preventing the expansion of viruses into susceptible populations is an increasing concern. with a few exceptions, available vaccines protecting against arboviral infections are nonexistent and current disease pre ...	2016	27664127
the evolutionary divergence of stat transcription factor in different anopheles species.	anopheles mosquito transmits plasmodium, the malaria causing parasite. different species of anopheles mosquito dominate in a particular geographical location and are capable of transmitting specific strains of plasmodium. it is important to understand the biology of different anophelines to control the parasite transmission. stat is an evolutionary conserved transcription factor that regulates the parasite development in african malaria vector anopheles gambiae. unlike drosophila and aedes aegyp ...	2017	27664587
evaluating the performance of infectious disease forecasts: a comparison of climate-driven and seasonal dengue forecasts for mexico.	dengue viruses, which infect millions of people per year worldwide, cause large epidemics that strain healthcare systems. despite diverse efforts to develop forecasting tools including autoregressive time series, climate-driven statistical, and mechanistic biological models, little work has been done to understand the contribution of different components to improved prediction. we developed a framework to assess and compare dengue forecasts produced from different types of models and evaluated t ...	2016	27665707
impact of micropollutants on the life-history traits of the mosquito aedes aegypti: on the relevance of transgenerational studies.	hazard assessment of chemical contaminants often relies on short term or partial life-cycle ecotoxicological tests, while the impact of low dose throughout the entire life cycle of species across multiple generations has been neglected. this study aimed at identifying the individual and population-level consequences of chronic water contamination by environmental concentrations of three organic micropollutants, ibuprofen, bisphenol a and benzo[a]pyrene, on aedes aegypti mosquito populations in e ...	2017	27667679
n6-(2-hydroxyethyl)-adenosine exhibits insecticidal activity against plutella xylostella via adenosine receptors.	the diamondback moth, plutella xylostella, is one of the most important pests of cruciferous crops. we have earlier shown that n6-(2-hydroxyethyl)-adenosine (hea) exhibits insecticidal activity against p. xylostella. in the present study we investigated the possible mechanism of insecticidal action of hea on p. xylostella. hea is a derivative of adenosine, therefore, we speculated whether it acts via p. xylostella adenosine receptor (pxador). we used rnai approach to silence pxador gene and used ...	2016	27668428
salivary gland proteome during adult development and after blood feeding of female anopheles dissidens mosquitoes (diptera: culicidae).	understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. anopheles dissidens (formerly anopheles barbirostris species a1) is a potential vector of plasmodium vivax in thailand. in this study, analyses of the proteomic profiles of female an. dissidens salivary glands during adult development and after blood feeding were carried out us ...	2016	27669021
spatial variations in dengue transmission in schools in thailand.	dengue is an important neglected tropical disease, with more than half of the world's population living in dengue endemic areas. good understanding of dengue transmission sites is a critical factor to implement effective vector control measures.	2016	27669170
global genetic diversity of aedes aegypti.	mosquitoes, especially aedes aegypti, are becoming important models for studying invasion biology. we characterized genetic variation at 12 microsatellite loci in 79 populations of ae. aegypti from 30 countries in six continents, and used them to infer historical and modern patterns of invasion. our results support the two subspecies ae. aegypti formosus and ae. aegypti aegypti as genetically distinct units. ae. aegypti aegypti populations outside africa are derived from ancestral african popula ...	2016	27671732
proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016.		2016	27677701
insights into zika virus history, human health effects, and control measures.		2016	27679523
new paradigms for virus detection, surveillance and control of zika virus vectors in the settings of southeast asia.	zika virus (zikv) has now become a global public health concern. the vectors for zikv are aedes aegypti and a. albopictus. both these mosquitoes are predominant in southeast asia and are also responsible for the spread of other arboviral diseases like dengue virus and chikungunya virus. the incidence of dengue has been increasing over the years and this is of concern to public health workers. simple laboratory tools for the detection of zikv is also lacking. in the absence of drugs and vaccine f ...	2016	27679623
isolation of zika virus imported from tonga into australia.	the globally emergent zika virus (zikv) is a threat to australia, given the number of imported cases from epidemic regions and the presence of competent mosquito vectors. we report the isolation of zikv from a female traveler who recently returned from tonga to brisbane, queensland, australia in 2016.	2016	27679739
climate change influences potential distribution of infected aedes aegypti co-occurrence with dengue epidemics risk areas in tanzania.	dengue is the second most important vector-borne disease of humans globally after malaria. incidence of dengue infections has dramatically increased recently, potentially due to changing climate. climate projections models predict increases in average annual temperature, precipitation and extreme events in the future. the objective of this study was to assess the effect of changing climate on distribution of dengue vectors in relation to epidemic risk areas in tanzania.	2016	27681327
kap surveys and dengue control in colombia: disentangling the effect of sociodemographic factors using multiple correspondence analysis.	during the last few decades, several studies have analyzed and described knowledge, attitudes, and practices (kap) of populations regarding dengue. however, few studies have applied geometric data analytic techniques to generate indices from kap domains. results of such analyses have not been used to determine the potential effects of sociodemographic variables on the levels of kap. the objective was to determine the sociodemographic factors related to different levels of kap regarding dengue in ...	2016	27682141
the golgi associated eri3 is a flavivirus host factor.	dengue virus (denv) is a mosquito-borne flavivirus classified into four serotypes (denv-1-4) that causes dengue fever (df), dengue hemorrhagic fever (dhf) or dengue shock syndrome (dss). an estimated 390 million people are at risk for infection with denv and there are no effective vaccines or therapeutics. we utilized rna chromatography coupled with quantitative mass spectrometry (qms) to identify host rna binding proteins (rbps) that interact with denv-2 rna. we identified eri3 (also prnpip and ...	2016	27682269
oviposition ecology and species composition of aedes spp. and aedes aegypti dynamics in variously urbanized settings in arbovirus foci in southeastern côte d'ivoire.	aedes mosquito-transmitted outbreaks of dengue and yellow fever have been reported from rural and urban parts of côte d'ivoire. the present study aimed at assessing aedes spp. oviposition ecology in variously urbanized settings within arbovirus foci in southeastern côte d'ivoire.	2016	27682270
discovery of novel viruses in mosquitoes from the zambezi valley of mozambique.	mosquitoes carry a wide variety of viruses that can cause vector-borne infectious diseases and affect both human and veterinary public health. although mozambique can be considered a hot spot for emerging infectious diseases due to factors such as a rich vector population and a close vector/human/wildlife interface, the viral flora in mosquitoes have not previously been investigated. in this study, viral metagenomics was employed to analyze the viral communities in culex and mansonia mosquitoes ...	2016	27682810
local mosquito-borne transmission of zika virus - miami-dade and broward counties, florida, june-august 2016.	during the first 6 months of 2016, large outbreaks of zika virus disease caused by local mosquito-borne transmission occurred in puerto rico and other u.s. territories, but local mosquito-borne transmission was not identified in the continental united states (1,2). as of july 22, 2016, the florida department of health had identified 321 zika virus disease cases among florida residents and visitors, all occurring in either travelers from other countries or territories with ongoing zika virus tran ...	2016	27684886
uv-b radiation reduces in vitro germination of metarhizium anisopliae s.l. but does not affect virulence in fungus-treated aedes aegypti adults and development on dead mosquitoes.	control of diurnal aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to uv-b radiation that might affect activity of conidia applied on the mosquito's surface.	2016	27685030
developmental pirna profiles of the invasive vector mosquito aedes albopictus.	in eukaryotic organisms, piwi-interacting rnas (pirnas) control the activities of mobile genetic elements and ensure genome maintenance. recent evidence indicates that pirnas are involved in multiple biological pathways, including transcriptional regulation of protein-coding genes, sex determination and even interactions between host and pathogens. aedes albopictus is a major invasive species that transmits a number of viral diseases in humans. ae. albopictus has the largest genome and the highe ...	2016	27686069
management of infection by the zika virus.	a panel of national experts was convened by the brazilian infectious diseases society in order to organize the national recommendations for the management of zika virus infection. the focus of this document is the diagnosis, both clinical and laboratorial, and appropriate treatment of the diverse manifestations of this infection, ranging from acute mild disease to guillain-barré syndrome and also microcephaly and congenital malformations.	2016	27686610
zika virus strains potentially display different infectious profiles in human neural cells.	the recent zika virus (zikv) epidemic has highlighted the poor knowledge on its physiopathology. recent studies showed that zikv of the asian lineage, responsible for this international outbreak, causes neuropathology in vitro and in vivo. however, two african lineages exist and the virus is currently found circulating in africa. the original african strain was also suggested to be neurovirulent but its laboratory usage has been criticized due to its multiple passages. in this study, we compared ...	2016	27688094
dengue: a clinicohaematological profile.	fifty cases of fever, clinically suspected to be dengue were studied.	2011	27688570
colour cues: effects of ipomoea plant extract on culex quinquefasciatus say gravid females in choosing oviposition site.	the interaction between plants and insects is dynamic, and may favour either the plant or the insect. plant chemicals are deeply implicated in this relationship and influence insect behaviour. here, we investigated the oviposition behaviour response of culex quinquefasciatus mosquitoes based on the colour cues produced by ipomoea cairica leaves extract. in this study, two sets of oviposition choice experiments were conducted: (1) single solution in a cage; and (2) multiple concentration solution ...	0	27688853
transcript abundance of photorhabdus insect-related (pir) toxin in manduca sexta and galleria mellonella infections.	in this study, we assessed pirab toxin transcription in photorhabdus luminescens laumondii (strain tt01) (enterobacteriaceae) by comparing mrna abundance under in vivo and in vitro conditions. in vivo assays considered both natural and forced infections with two lepidopteran hosts: galleria mellonella and manduca sexta. three portals of entry were utilized for the forced infection assays: (a) integument; (b) the digestive route (via mouth and anus); and (c) the tracheal route (via spiracles). we ...	2016	27690103
research contributing to improvements in controlling florida's mosquitoes and mosquito-borne diseases.	research on mosquitoes and mosquito-borne diseases has contributed to improvements in providing effective, efficient, and environmentally proper mosquito control. florida has benefitted from several research accomplishments that have increased the state's mosquito control capabilities. research with florida's mosquitoes has resulted in the development of ecologically sound management of mosquito impoundments on florida's east coast. this strategy, called rotational impoundment management (rim), ...	2016	27690112
wolbachia restricts insect-specific flavivirus infection in aedes aegypti cells.	mosquito-borne viruses are known to cause disease in humans and livestock and are often difficult to control due to the lack of specific antivirals and vaccines. the wolbachia endosymbiont has been widely studied for its ability to restrict positive-strand rna virus infection in mosquitoes, although little is known about the precise antiviral mechanism. in recent years, a variety of insect-specific viruses have been discovered in mosquitoes and an interaction with mosquito-borne viruses has been ...	2016	27692043
sexual chemoecology of mosquitoes (diptera, culicidae): current knowledge and implications for vector control programs.	mosquitoes (diptera: culicidae) act as vectors of medical and veterinary importance, due to their ability to transmit many pathogens and parasites. renewed interest has been recently devoted to the potential of sterile insect technique (sit) for mosquito suppression. however, the success of the sit is mostly dependent on the ability of sterile males to compete for mates with the wild ones in the field. nevertheless, little is known on the sexual chemical ecology of mosquitoes, with special refer ...	2017	27692501
excito-repellency of citrus hystrix dc leaf and peel essential oils against aedes aegypti and anopheles minimus (diptera: culicidae), vectors of human pathogens.	the essential oils of kaffir lime (citrus hystrix dc.) at four different concentrations (0.5, 1.0, 2.5, and 5.0% v/v) were studied for their repellency, excitation, and knockdown properties against laboratory strains of aedes aegypti (l.) and anopheles minimus theobald using an excito-repellency test system. both contact and noncontact escape responses to leaf- and peel-derived kaffir lime oils were observed. comparing unadjusted escape responses for an. minimus, leaf oil had strong combined irr ...	2016	27694144
disruption of phenylalanine hydroxylase reduces adult lifespan and fecundity, and impairs embryonic development in parthenogenetic pea aphids.	phenylalanine hydroxylase (pah) is a key tyrosine-biosynthetic enzyme involved in neurological and melanin-associated physiological processes. despite extensive investigations in holometabolous insects, a pah contribution to insect embryonic development has never been demonstrated. here, we have characterized, for the first time, the pah gene in a hemimetabolous insect, the aphid acyrthosiphon pisum. phylogenetic and sequence analyses confirmed that appah is closely related to metazoan pah, exhi ...	2016	27694983
climate change influences on the global potential distribution of the mosquito culex quinquefasciatus, vector of west nile virus and lymphatic filariasis.	rapid emergence of most vector-borne diseases (vbds) may be associated with range expansion of vector populations. culex quinquefasciatus say 1823 is a potential vector of west nile virus, saint louis encephalitis virus, and lymphatic filariasis. we estimated the potential distribution of cx. quinquefasciatus under both current and future climate conditions. the present potential distribution of cx. quinquefasciatus showed high suitability across low-latitude parts of the world, reflecting the c ...	2016	27695107
massive yet grossly underestimated global costs of invasive insects.	insects have presented human society with some of its greatest development challenges by spreading diseases, consuming crops and damaging infrastructure. despite the massive human and financial toll of invasive insects, cost estimates of their impacts remain sporadic, spatially incomplete and of questionable quality. here we compile a comprehensive database of economic costs of invasive insects. taking all reported goods and service estimates, invasive insects cost a minimum of us$70.0 billion p ...	2016	27698460
zika: an old virus with a new face.	zika virus is a mosquito-borne flavivirus that represents a public health emergency at the ongoing epidemic. this obscure virus was limited to sporadic cases in africa and asia, until the emergence of zika virus in brazil in 2015, when it rapidly spread throughout the americas. most zika virus infections are subclinical or characterized by mild febrile illness. however, neurological complications, including guillain-barré syndrome in adults, and congenital anomalies, including microcephaly in ba ...	2016	27703544
rapid and high throughput molecular identification of diverse mosquito species by high resolution melting analysis.	mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. the correct identification of mosquitoes is paramount to the control of the diseases that they transmit. however, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among species complexes (sibling and cryptic species). high resolution melting (hrm) analyses, a closed-tube, ...	2016	27703667
widespread rift valley fever emergence in senegal in 2013-2014.	rift valley fever (rvf), which caused epizootics and epidemics among human and livestock populations, occurred in senegal in 2013-2014. a multidisciplinary field investigation was carried out in 3 regions of senegal. we found 11 confirmed human cases of rift valley fever, including severe cases with encephalitis and retinitis, 1 pool of mosquito (aedes ochraceus), and 52 animals tested positive for the disease. symptoms such as encephalitis and macular retinitis were the most severe cases report ...	2016	27704007
full genome sequence and sfrna interferon antagonist activity of zika virus from recife, brazil.	the outbreak of zika virus (zikv) in the americas has transformed a previously obscure mosquito-transmitted arbovirus of the flaviviridae family into a major public health concern. little is currently known about the evolution and biology of zikv and the factors that contribute to the associated pathogenesis. determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative proces ...	2016	27706161
first detection of natural infection of aedes aegypti with zika virus in brazil and throughout south america.	zika virus (zikv) has caused a major epidemic in brazil and several other american countries. zikv is an arbovirus whose natural vectors during epidemics have been poorly determined. in this study, 1,683 mosquitoes collected in the vicinity of zikv suspected cases in rio de janeiro, brazil, from june 2015 to may 2016 were screened for natural infection by using molecular methods. three pools of aedes aegypti were found with the zikv genome, one of which had only one male. this finding supports t ...	2016	27706382
detecting multiple dna human profile from a mosquito blood meal.	criminal traces commonly found at crime scenes may present mixtures from two or more individuals. the scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation. the aim of the study was to obtain a single aedes aegypti mosquito profile from a human dna mixture containing genet ...	2016	27706606
field comparisons of the gravid aedes trap (gat) and bg-sentinel trap for monitoring aedes albopictus (diptera: culicidae) populations and notes on indoor gat collections in vietnam.	we report on the use of the gravid aedes trap (gat) as a surveillance device for aedes albopictus (skuse) relative to the bg-sentinel (bgs) trap in field studies conducted in trenton, nj, and on hammond island, queensland, australia. a parallel study conducted in nha trang, vietnam, assessed the use of the gat as an indoor surveillance device as well as the use of canola oil as a noninsecticide killing agent. in trenton and hammond island, the gat collected fewer male (0.40 ± 0.12 and 0.43 ± 0.3 ...	2017	27707983
zika: the origin and spread of a mosquito-borne virus.	to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic).	2016	27708473
detecting guillain-barré syndrome caused by zika virus using systems developed for polio surveillance.		2016	27708476
persistent infection by wolbachia walbb has no effect on composition of the gut microbiota in adult female anopheles stephensi.	the bacteria in the midgut of anopheles stephensi adult females from laboratory colonies were studied by sequencing the v4 region of 16s rrna genes, with respect to three experimental factors: stable or cured wolbachia infection; sugar or blood diet; and age. proteobacteria and bacteroidetes dominated the community [>90% of operational taxonomic units (otus)]; most taxa were in the classes flavobacteriia, gammaproteobacteria, and alphaproteobacteria, and were assigned to elizabethkingia (46.9%), ...	2016	27708633
emergence of epidemic dengue-1 virus in the southern province of sri lanka.	dengue is a frequent cause of acute febrile illness with an expanding global distribution. since the 1960s, dengue in sri lanka has been documented primarily along the heavily urbanized western coast with periodic shifting of serotypes. outbreaks from 2005-2008 were attributed to a new clade of denv-3 and more recently to a newly introduced genotype of denv-1. in 2007, we conducted etiologic surveillance of acute febrile illness in the southern province and confirmed dengue in only 6.3% of febri ...	2016	27711206
an analysis of the potential impact of climate change on dengue transmission in the southeastern united states.	dengue fever, caused by a mosquito-transmitted virus, is an increasing health concern in the americas. meteorological variables such as temperature and precipitation can affect disease distribution and abundance through biophysical impacts on the vector and on the virus. such tightly coupled links may facilitate further spread of dengue fever under a changing climate. in the southeastern united states, the dengue vector is widely established and exists on the current fringe of dengue transmissio ...	2017	27713106
transcriptome comparison analysis of ostrinia furnacalis in four developmental stages.	the asian corn borer, ostrinia furnacalis, is one of the most destructive pests of maize and causes huge losses in maize yield each year. in order to characterize the different developmental stages, a high-throughput sequencing platform was employed to perform de novo transcriptome assembly and gene expression analysis for the egg, larva, pupa and adult stages. approximately 185 million reads were obtained, trimmed, and assembled into 42,638 unigenes with an average length of 801.94 bp and an n5 ...	2016	27713521
glycoside hydrolases family 20 (gh20) represent putative virulence factors that are shared by animal pathogenic oomycetes, but are absent in phytopathogens.	although interest in animal pathogenic oomycetes is increasing, the molecular basis mediating oomycete-animal relationships remains virtually unknown. crinkler (crn) genes, which have been traditionally associated with the cytotoxic activity displayed by plant pathogenic oomycetes, were recently detected in transcriptome sequences from the entomopathogenic oomycete lagenidium giganteum, suggesting that these genes may represent virulence factors conserved in both animal and plant pathogenic oomy ...	2016	27716041
zika virus on a spreading spree: what we now know that was unknown in the 1950's.	zika virus (zikv) is a mosquito-borne flavivirus that is transmitted through the bite of aedes spp mosquitoes and less predominantly, through sexual intercourse. prior to 2007, zikv was associated with only sporadic human infections with minimal or no clinical manifestations. recently the virus has caused disease outbreaks from the pacific islands, the americas, and off the coast of west africa with approximately 1.62 million people suspected to be infected in more than 60 countries around the g ...	2016	27716242
genetic variability and spatial distribution in small geographic scale of aedes aegypti (diptera: culicidae) under different climatic conditions in northeastern brazil.	the study of the genetic structure of aedes aegypti is essential to understanding their population dynamics as well as for the analysis of factors responsible for their resistance and ecological adaptation. the use of molecular markers in identifying differences amongst populations of ae. aegypti in different geographical areas as well as the temporal variation of the vector populations has contributed to the improvement of vector control strategies. the present study aims to determine the genet ...	2016	27716392
analyzing climate variations at multiple timescales can guide zika virus response measures.	the emergence of zika virus (zikv) in latin america and the caribbean in 2014-2016 occurred during a period of severe drought and unusually high temperatures, conditions that have been associated with the 2015-2016 el niño event, and/or climate change; however, no quantitative assessment has been made to date. analysis of related flaviviruses transmitted by the same vectors suggests that zikv dynamics are sensitive to climate seasonality and longer-term variability and trends. a better understan ...	2016	27716414
mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats.	mosquitoes are insects of interest because several species vector disease-causing pathogens to humans and other vertebrates. we previously reported that mosquitoes from long-term laboratory cultures require living bacteria in their gut to develop, but development does not depend on particular species of bacteria. here, we focused on three distinct but interrelated areas of study to better understand the role of bacteria in mosquito development by studying field and laboratory populations of aede ...	2016	27718295
an updated review of zika virus.	the current outbreak of zika virus (zikv) in south america is one of the most serious public health emergencies since the ebola outbreak of west africa [2014]. zikv belongs to the flaviviridae family and has two lineages (asian and african). the virus was first discovered in uganda [1947] and the first human infection was identified in nigeria [1952]. the current epidemic is the third of its type after that of yap island, micronesia [2007] and french polynesia [2013]. phylogenetic studies reveal ...	2016	27721110
darwin's finches treat their feathers with a natural repellent.	darwin's finches are highly innovative. recently we recorded for the first time a behavioural innovation in darwin's finches outside the foraging context: individuals of four species rubbed leaves of the endemic tree psidium galapageium on their feathers. we hypothesised that this behaviour serves to repel ectoparasites and tested the repellency of p. galapageium leaf extracts against parasites that negatively affect the fitness of darwin's finches, namely mosquitoes and the invasive hematophago ...	2016	27721475
epidemiological and molecular characterization of dengue viruses imported into guangzhou during 2009-2013.	dengue virus causes one of the most significant infectious diseases in tropical and subtropical regions, notable number of which is imported into china every year.	2016	27722053
multiple insecticide resistance in the malaria vector anopheles funestus from northern cameroon is mediated by metabolic resistance alongside potential target site insensitivity mutations.	despite the recent progress in establishing the patterns of insecticide resistance in the major malaria vector anopheles funestus, central african populations of this species remain largely uncharacterised. to bridge this important gap and facilitate the implementation of suitable control strategies against this vector, we characterised the resistance patterns of an. funestus population from northern cameroon.	2016	27723825
infection with plasmodium berghei ookinetes alters protein expression in the brain of anopheles albimanus mosquitoes.	the behaviour of anopheles spp. mosquitoes, vectors for plasmodium parasites, plays a crucial role in the propagation of malaria to humans. consequently, it is important to understand how the behaviour of these mosquitoes is influenced by the interaction between the brain and immunological status. the nervous system is intimately linked to the immune and endocrine systems. there is evidence that the malaria parasite alters the function of these systems upon infecting the mosquito. although there ...	2016	27724938
forecasting united states heartworm dirofilaria immitis prevalence in dogs.	this paper forecasts next year's canine heartworm prevalence in the united states from 16 climate, geographic and societal factors. the forecast's construction and an assessment of its performance are described.	2016	27724981