Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacterial populations associated with meat from the deboning room of a high throughput red meat abattoir. | developing countries are faced with high incidences of food poisoning outbreaks, with obvious economic consequences. in highly perishable foodstuffs such as fresh red meat the threat of food poisoning is particularly intense. in this study, red meat samples were collected from a deboning room of a high throughput abattoir. the samples were analysed for the presence of bacillus cereus., staphylococcus aureus., pseudomonas spp., listeria monocytogenes., escherichia coli and salmonella spp. the aer ... | 2004 | 22060877 |
| production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples. | a total of 51 sucuk samples, obtained from different regions of turkey, were examined for lactic acid bacteria (lab) strains producing putative bacteriocin-like metabolites. for detection of antagonistic activity, agar spot and well diffusion assay tests were used. lactobacillus sake lb790, listeria monocytogenes li6, staphylococcus aureus st44 and escherichia coli nrrl b-3704 were used as indicator organisms. strains having antimicrobial activity were also tested against clostridium perfringens ... | 2000 | 22060908 |
| effect of food grade organic acids on inoculated s. aureus, l. monocytogenes, e. coli and s. typhimurium in sheep/goat meat stored at refrigeration temperature. | sheep/goat forequarters procured from freshly slaughtered animals were decontaminated with hot water and inoculated with staphylococcus aureus, listeria monocytogenes, escherichia coli and salmonella typhimurium. the forequarters were individually spray washed with 2% lactic acid and 1.5% acetic + 1.5% propionic acid combination. total viable count (tvc) of the treated meat samples was reduced by about 0.52 and 1.16 log units with marginal changes in colour and odour scores. inoculated organisms ... | 2004 | 22061013 |
| inhibition of listeria monocytogenes by a bacteriocinogenic lactobacillus sake strain in modified atmosphere-packaged brazilian sausage. | lactobacillus sake 2a is a bacteriocinogenic strain isolated from "lingüiça frescal", a brazilian sausage. the combined effect of modified-atmosphere (ma) packaging (100% co(2) and 50% co(2)/50% n(2)) and addition of l. sake 2a on inhibition of growth of listeria monocytogenes was evaluated in "lingüiça" stored at 6 °c. by the end of the first week, the inhibition of l. monocytogenes due to ma was significant (p?0.05) while the presence of l. sake 2a did not influence significantly the growth of ... | 2002 | 22061076 |
| production of mortadella: behavior of listeria monocytogenes during processing and storage conditions. | the presence of listeria monocytogenes in ready-to-eat meat products is cause of concern to the food industry as well as to health authorities. studies were conducted to evaluate the presence of l. monocytogenes in mortadellas acquired at retail stores and to evaluate the fate of two levels of a l. monocytogenes pool spiked in two different formulations of the product, cooked under commercial conditions and stored at refrigeration (7°c) and room temperature (25°c). among the samples collected at ... | 2001 | 22061161 |
| listeria monocytogenes: occurrence in beef and identification of the main contamination points in processing plants. | this study aimed to establish the occurrence of listeria spp., especially l. monocytogenes and its main serotypes, in beef and processing plants. a total of 443 samples were obtained from equipment, installations and products from 11 meat processing establishments from paraná state, brazil. all samples were analyzed using usda methodology for listeria spp. detection, followed by species identification. the occurrence of listeria spp. in the samples was 38.1% of which 51.4% were from equipment, 3 ... | 2007 | 22061233 |
| antilisterial activity of lactic acid bacteria isolated from "alheiras" (traditional portuguese fermented sausages): in situ assays. | a total of 226 lactic acid bacteria (lab) isolated from "alheira", a traditional portuguese fermented sausage, were screened for antagonistic activity against some pathogenic microorganisms, including listeria monocytogenes. the objective was to isolate lab with antibacterial activity from "alheiras" and to select strains that could be used in "alheira" production. isolates displaying antibacterial activity against listeria innocua and l. monocytogenes were investigated for the nature of the ant ... | 2007 | 22061260 |
| effect of a bacteriocin produced by pediococcus acidilactici against listeria monocytogenes and clostridium perfringens on spanish raw meat. | the inhibitory effect of a bacteriocin, produced by pediococcus acidilactici, against listeria monocytogenes and clostridium perfringens on spanish raw meat surface, was evaluated by in situ assays. samples were incubated with the bacteriocin and then with a culture of the pathogenic bacteria. the treatment with 500, 1000 or 5000 bacteriocin units/ml (bu/ml) reduced the counts of l. monocytogenes after storage at 15°c during 72h by 1, 2 or 3 log cycles and with 1000 or 5000 bu/ml after storage a ... | 2006 | 22061374 |
| a note on the incidences of salmonella spp., listeria spp. and escherichia coli o157:h7 serotypes in turkish sausage (soudjouck). | the incidence of salmonella spp., listeria monocytogenes and escherichia coli o157:h7 was determined in 100 turkish sausage (soudjouck) samples collected from shops and markets in the afyon province, turkey. salmonella spp. were detected in 7% of the samples. all of the isolates were s. enterica paratyphi b. in addition, listeria spp. were detected in 9% of the samples. its distribution was 7% l. monocytogenes and 1% each of l. ivanovii and l. innocua. serological study of the seven l. monocytog ... | 2006 | 22061388 |
| effects of chemical hurdles on microbiological and oxidative stability of a cooked cured emulsion type meat product. | two combinations of hurdles, 2.0% lactate+0.5% acetate or 2.0% lactate+0.25 % glucono-delta-lactone (gdl), were both found to prevent growth of listeria monocytogenes inoculated onto sliced saveloys manufactured with 60 or 150 ppm nitrite. the saveloys were packed in modified atmosphere (80% n(2)/20% co(2)) using a film with low oxygen transmission rate (0.45 cm(3)/m(2)/atm/24 h) and stored at 5 or 10°c for up to 4 weeks. changes in red colour (measured as minolta a-values) and lipid oxidation [ ... | 2000 | 22061582 |
| new mild technologies in meat processing: high pressure as a model technology. | as a consequence of market globalization, the production and manufacture of meat products is at a stage of innovative dynamics. consumers demand high quality and convenient meat products, with natural flavour and taste, and very much appreciate the fresh appearance of minimally processed food. to harmonize or to blend all these demands without compromising safety, it is necessary to implement new preservation technologies in the meat industry and in the food industry in general. high hydrostatic ... | 2002 | 22061612 |
| bacteriological safety issues in red meat and ready-to-eat meat products, as well as control measures. | the importance of eschericha coli o157, listeria monocytogenes and salmonella typhimurium dt104 as meat-borne pathogens is well established. pathogenic bacteria such as aeromonas spp., arcobacter spp., psychrotrophic bacillus cereus, campylobacter spp., clostridium botulinum and non-invasive listeria monocytogenes can be regarded as rookies, but not yet firmly associated with today's production of red meat and meat products. the development of pcr and other dna-based techniques will shed new lig ... | 2002 | 22061614 |
| use of irradiation to control foodborne pathogens and extend the refrigerated market life of rabbit meat. | this study set out to evaluate the microbiological status of rabbit meat and the possibility of using irradiation to control foodborne pathogenic bacteria and extend the refrigerated storage life of meat. rabbit meat samples were ? irradiated at doses of 0, 1.5 and 3 kgy. the samples were stored at refrigeration temperature, then the effects of irradiation and storage on their microbiological, chemical and sensory properties were studied. irradiation at 1.5 kgy significantly reduced the counts o ... | 2004 | 22061802 |
| utilization of microbes to process and preserve meat. | this paper discusses how, and to what extent, the addition of microorganisms to meats helps to meet the needs of consumers and industry. lactic acid bacteria adapted to meats improve the safety of fermented sausages by means of acid formation. using selected strains, the safety of certain non-fermented, perishable meat products may be improved without affecting their shelf life. certain bacteriocin-forming cultures may reduce the levels of listeria monocytogenes in some meat products significant ... | 2000 | 22061897 |
| microbiological contamination of raw beef trimmings and ground beef. | to survey the microbiological quality of beef trimmings and final-ground beef, samples were collected from eight commercial grinding facilities, including trimmings from fed-cattle, culled-beef cows, culled-dairy cows, imported-beef trimmings and finished-ground products. trim samples (core and purge) and ground product samples (n=586) were evaluated for aerobic plate (apc), total coliform (tcc), escherichia coli (ecc) and staphylococcus aureus counts and the presence of salmonella spp. and list ... | 2000 | 22061902 |
| traditional dry fermented sausages produced in small-scale processing units in mediterranean countries and slovakia. 1: microbial ecosystems of processing environments. | microbial ecosystems were surveyed in 314 environmental samples from 54 southern and eastern european small-scale processing units (pus) manufacturing traditional dry fermented sausages. the residual microflora contaminating the surfaces and the equipment were analysed after cleaning and disinfection procedures. all the pu environments were colonised at various levels by spoilage and technological microflora with excessive contamination levels in some of the pus. sporadic contamination by pathog ... | 2007 | 22061943 |
| antioxidant and antimicrobial effects of four garlic-derived organosulfur compounds in ground beef. | the antioxidant and antimicrobial protection of diallyl sulfide (das), diallyl disulfide (dads), s-ethyl cysteine (sec), n-acetyl cysteine (nac) in ground beef against discoloration, lipid oxidation and microbial contamination were studied. the exogenous addition of these garlic-derived organosulfur compounds significantly delayed both oxymyoglobin and lipid oxidations (p<0.05). the antioxidant protection from these organosulfur compounds was dose-dependent (p<0.05), and showed significantly gre ... | 2003 | 22061980 |
| effect of nisin and its combination with sodium chloride on the survival of listeria monocytogenes added to raw buffalo meat mince. | antilisterial activity of nisin (nisaplin), alone at concentrations of 400 and 800 iu/g and in combination with 2% sodium chloride was incorporated in raw buffalo meat mince. samples of the raw meat mince were inoculated with 10(3) colony forming units (cfu)/g of l. monocytogenes and stored at 4°c for 16 days and at 37°c for 36 h. initial estimates of ph, extract release volume, mesophilic and psychrophilic counts were found to be 5.74, 48 ml, 3.5×10(5) and 1.0×10(5) cfu/g of meat, respectively. ... | 2000 | 22062071 |
| challenges to meat safety in the 21st century. | the safety of meat has been at the forefront of societal concerns in recent years, and indications exist that challenges to meat safety will continue in the future. major meat safety issues and related challenges include the need to control traditional as well as "new," "emerging," or "evolving" pathogenic microorganisms, which may be of increased virulence and low infectious doses, or of resistance to antibiotics or food related stresses. other microbial pathogen related concerns include cross- ... | 2008 | 22062090 |
| microbial safety of meat in the european union. | the two most frequently reported zoonotic diseases in humans in the eu in 2005 were campylobacter and salmonella infections with incidences of 51.6 and 38.2 cases per 100,000 population, respectively. reported human infections caused by yersinia spp., verocytotoxigenic escherichia coli, and listeria monocytogenes had comparably lower incidences of 2.6, 1.2 and 0.3 cases per 100,000 population, respectively. meat and meat products are important sources for these infections but knowledge on exactl ... | 2008 | 22062091 |
| inhibition of salmonella sp. listeria monocytogenes and staphylococcus aureus in cooked ham by combining antimicrobials, high hydrostatic pressure and refrigeration. | recontamination of ready-to-eat products such as cooked ham during post-processing may be the cause of outbreaks of food-borne disease. the effectiveness of the combination of high pressure processing (hpp) at 600mpa with the natural antimicrobials nisin and potassium lactate has been evaluated in sliced cooked ham spiked with 4logcfu/g of salmonella sp., listeria monocytogenes and staphylococcus aureus after 3-months of storage at 1 and 6°c. in non-hpp sliced cooked ham, the addition of nisin p ... | 2008 | 22062095 |
| molecular tracking of listeria monocytogenes in an iberian pig abattoir and processing plant. | the environment and products from an iberian pig abattoir and processing plant were sampled to investigate the prevalence and genetic diversity of listeria monocytogenes. the organism was not detected in the pig carcasses prior to processing. fresh and dry-cured pork did show detectable levels, always ranging below 100cfu per gram. a total of 163 l. monocytogenes isolates collected during one year were characterized by pcr-based serotyping and pulsed-field gel electrophoresis (pfge) restriction ... | 2008 | 22062102 |
| effect of citric acid on the radiation resistance of listeria monocytogenes and frankfurter quality factors. | listeria monocytogenes is a common contaminant of ready-to-eat meat products, including frankfurters. ionizing (gamma) radiation can eliminate l. monocytogenes from frankfurters. citric acid (ca) is an antioxidant synergist and anti-microbial agent that can be applied to the surfaces of cured meat products prior to packaging. the effect of ca on the radiation resistance of l. monocytogenes that was surface-inoculated onto frankfurters was determined. the d(10) values, the radiation doses require ... | 2003 | 22062395 |
| comparison of electrolyzed oxidizing water with other antimicrobial interventions to reduce pathogens on fresh pork. | to date, the effectiveness of electrolyzed oxidizing (eo) water against bacteria associated with fresh pork has not been determined. using a hand-held, food-grade garden sprayer, distilled water (w), chlorinated water (cl; 25 ppm), 2% lactic acid (la), acidic eo water (eoa), or "aged" acidic eo water (aeoa; stored at 4 °c for 24 h) was sprayed (15 s) onto pork bellies inoculated with feces containing listeria monocytogenes (lm), salmonella typhimurium (st), and campylobacter coli (cc). remaining ... | 2004 | 22062415 |
| occurrence of escherichia coli o157:h7, listeria monocytogenes, salmonella and campylobacter spp. on beef carcasses in northern ireland. | a survey of beef carcasses was conducted in all 10 european community approved abattoirs in northern ireland to determine the incidence of escherichia coli o157:h7. analyses were based on excised samples of neck meat taken less than 48 h post-kill. overall, 780 carcasses were sampled and all were negative for e. coli o157:h7. a sub-set of samples was analysed for the presence of listeria monocytogenes (n=200), salmonella (n=200) and campylobacter spp.(n=100). l. monocytogenes was not detected bu ... | 2001 | 22062423 |
| behaviour of listeria monocytogenes in raw sausages (merguez) in presence of a bacteriocin-producing lactococcal strain as a protective culture. | the effectiveness of a bacteriocin produced by lactococcus lactis subsp. lactis m in reducing population level and growth of listeria monocytogenes atcc 7644 in fermented merguez sausage was examined. two different formulas (with or without added nitrites) were assayed and predetermined numbers of listeria (ca 10(6) cfu g(-1)) were added to sausage mixture. the effect of in situ production of the bacteriocin by lactococcus lactis m on listeria monocytogenes atcc 7644 during fermentation and stor ... | 2003 | 22062517 |
| growth of listeria monocytogenes on vacuum-packaged horsemeat for human consumption. | in order to investigate the likelihood of listeria monocytogenes (serotype 4b, atcc 19115) growth on vacuum-packaged horsemeat at refrigeration temperature, fourteen horsemeat surface/volume homogeneous 150 g weight pieces were superficially inoculated with serotype 4b l. monocytogenes and vacuum packaged. the samples were stored at 4±1 °c. two pieces (one for ph determination and one for l. monocytogenes counts) were examined at days 0, 7, 14, 21, 28, 35 and 42. surface ph did not show signific ... | 2004 | 22062545 |
| role of quantitative risk assessment and food safety objectives in managing listeria monocytogenes on ready-to-eat meats. | listeria monocytogenes may be found on ready-to-eat (rte) meats, posing a public health risk. to minimize the public health impact, an appropriate level of protection (alop) can be established for a population with respect to l. monocytogenes, and ideally should be based on a scientific assessment of the risk, as well as societal and economic factors. food safety systems can be based on meeting the alop. food safety objectives (fso) provide a link between the alop and performance objectives that ... | 2006 | 22062717 |
| influence of rosemary-tocopherol/packaging combination on meat quality and the survival of pathogens in restructured irradiated pork loins. | irradiated restructured pork loins treated with rosemary-tocopherol/double-packaging had lower tbars values than vacuum-packaged control after 10 days of refrigerated storage. the rosemary-tocopherol combination, however, had no effect on the production of sulfur volatiles responsible for the irradiation off-odor, and color changes in irradiated pork. v7/a3 double-packaging was effective in reducing the sulfur volatiles significantly. rosemary-tocopherol combination was highly effective in reduc ... | 2006 | 22062849 |
| antagonistic effect on listeria monocytogenes and l. innocua of a bacteriocin-like metabolite produced by lactic acid bacteria isolated from sucuk. | two lactobacilli and four pediococci strains producing bacteriocin-like metabolities isolated from sucuk were tested with agar spot tests and well diffusion assays for their inhibitory activity against 16 listeria strains, also isolated from sucuk. the production of organic acids and hydrogen peroxide limited, l. sake lb 706 (used as a bacteriocin producer strain) and the isolated lactic acid bacteria (lab) showed inhibitory activity against all of the listeria strains, while l. sake lb 706-a (u ... | 2001 | 22062969 |
| characterisation of naturally fermented sausages produced in the north east of italy. | in the friuli venezia giulia region, in the north east of italy, a traditional fermented sausage is produced without the use of microbial starters. it is characterized at the end of the ripening period by accentuated acidity, slight sourness and elastic, semi-hard consistency. in this study, three fermentations, carried out in different seasons (winter, spring and summer) were followed analyzing the microbiological, physicochemical and sensory aspects of this product. the sausages were character ... | 2005 | 22062975 |
| microbial heat resistance of listeria monocytogenes and the impact on ready-to-eat meat quality after post-package pasteurization. | several methods using bactericides, hydrostatic pressure, and post-package pasteurization technologies to control listeria monocytogenes (lm) in ready-to-eat meats have been attempted. in addition to controlling lm contamination, any newly developed technology must have minimal effects on organoleptic properties. the objectives of this study were to: (1) determine the heat resistance of lm in two brands (a and b) of bologna differing in formulations, and, (2) evaluate the effects of post-package ... | 2006 | 22063046 |
| the sensory acceptability of cooked meat products treated with a protective culture depends on glucose content and buffering capacity: a case study with lactobacillus sakei 10a. | biopreservation has been proven to be a promising natural preservation technique, but the impact of protective cultures on the sensory properties of cooked meat products (cmp) is not well documented. this work presents a case study on the protective culture lactobacillus sakei 10a to obtain a clear view on the real consequences of using protective cultures on the sensory quality of cmp. a preliminary screening study on 13 different cmp and more elaborate application trials at 7°c on vacuum packa ... | 2006 | 22063058 |
| antilisterial activity of lactic acid bacteria inoculated on cooked ham. | this study was conducted to evaluate the ability of lactobacillus sakei 1, a bacteriocin-producing (bac(+)) lactic acid bacterium (lab), isolated from brazilian fresh pork sausage to inhibit two listeria monocytogenes strains (serotypes 4b and 1/2a) on cooked, sliced vacuum-packaged ham. l. sakei atcc 15521 was used as a non-bacteriocin producer (bac(-)). l. monocytogenes (ca. 2 logcfu/ml) and lab (ca. 6 logcfu/ml) were inoculated on the sterilized ham, vacuum-sealed and incubated at 8°c for 10 ... | 2006 | 22063215 |
| evaluation of sodium lactate as a replacement for conventional chemical preservatives in comminuted sausages inoculated with listeria monocytogenes. | sodium lactate (sl) as a potential replacer for potassium sorbate (ps) or sodium benzoate (sb) in comminuted sausages was evaluated. sausages manufactured with 3.3% sl were compared with a control and 0.05 or 0.1% of ps and sb with regard to its influence on changes of chemical composition, physico-chemical and textural properties, and the growth of inoculated listeria monocytogenes (lm) stored at 4 °c for up to 8 weeks. the sausages contained 62-64% moisture, 15-17% fat and 12-14% protein with ... | 2003 | 22063246 |
| use of ionizing radiation doses of 2 and 4kgy to control listeria spp. and escherichia coli o157:h7 on frozen meat trimmings used for dry fermented sausage production. | this study evaluated survival of listeria spp. (four-strain mixture of listeria innocua plus a non-virulent listeria monocytogenes strain) and escherichia coli o157:h7 strain atcc 43888 during fermentation and ripening of greek dry sausages formulated from meat and pork fat trimmings previously inoculated with ca. 6logcfug(-1) of the target bacteria and then irradiated in frozen (-25°c) blocks at doses of 0 (control), 2 or 4kgy. irradiation of the trimmings at 2kgy reduced initial contamination ... | 2005 | 22063296 |
| purification and characterization of a bacteriocin produced by leuconostoc mesenteroides e131. | leuconostoc mesenteroides e131, isolated from greek traditional fermented sausage, prepared without the addition of starters, produces a bacteriocin which is active against the pathogen listeria monocytogenes. the bacteriocin was purified by 50% ammonium sulphate precipitation, cation exchange, and reverse-phase chromatography. bacteriocin is active at ph values between 4.0 and 9.0 and retains activity after incubation for 1h at 100°c. proteolytic enzymes inactivated the bacteriocin after 1h of ... | 2008 | 22063322 |
| microbial profiles of frozen trimmings and silver sides prepared at indian buffalo meat packing plants. | to assess microbiological quality of buffalo meat trimmings (tt=114) and silver sides (ss=41), samples were collected from four different indian meat packing plants. the aim of this study was: (i) to evaluate standard plate count (spc), psychrotrophic count (ptc), enterococcus feacalis count (efc), staphylococcus aureus count (sac) and escherichia coli count (ecc) and the presence of salmonella spp. and listeria monocytogenes; and (ii) also to determine vero toxic e. coli (vtec) by polymerase ch ... | 2008 | 22063348 |
| characterization of lactic acid bacteria isolated from a greek dry-fermented sausage in respect of their technological and probiotic properties. | a total of 147 lactic acid bacteria was isolated from two types of naturally fermented dry sausages at four different stages of the ripening process studied in order to select the most suitable strains according to their technological characteristics including probiotic properties and antimicrobial activity against food-borne pathogens. identification of the isolates revealed that 90% were lactobacilli, 4% enterococci, 3% pediococcus sp. and sporadic isolates of weissella viridescens, leuconosto ... | 2003 | 22063449 |
| enhancing the antilisterial effect of lactobacillus curvatus cwbi-b28 in pork meat and cocultures by limiting bacteriocin degradation. | this work focused on listeria monocytogenes growth inhibition and growth rebound in raw and cooked pork meat inoculated with lactobacillus curvatus strains. during storage of raw meat homogenates in the presence of the bacteriocin-producing strain lactobacillus curvatus cwbi-b28wt, the listeria monocytogenes cfu count was initially reduced to an undetectable level, but a growth rebound occurred after two weeks, coinciding with loss of 70% of the bacteriocin activity present at the end of week 2. ... | 2008 | 22063576 |
| effect of pork lard content on the chemical, microbiological and sensory properties of a typical fermented meat product (pitina) obtained from alpagota sheep. | the aim was to investigate the physicochemical, microbiological and sensory properties of pitina, a typical fermented meat product and evaluate the effect of two levels of pork lard content (low fat, lf, 10% vs. high fat, hf, 30%) on its attributes. hf attained lower ph than lf pitina, which reached lower water activity. lab comprised the major flora with substantial counts of micrococci, enterococci and mould and yeast. gram negative enterobacteria were recovered as coliforms and faecal colifor ... | 2008 | 22063596 |
| the effects of sodium lactate and starter cultures on ph, lactic acid bacteria, listeria monocytogenes and salmonella spp. levels in pure chicken dry fermented sausage. | two starter cultures (a and b) and seven sodium lactate concentrations were evaluated for chicken raw dry-fermented sausage processing. starter culture b contained more lactic acid bacteria and less staphylococci than starter a. their effects on acidification and inhibition of pathogens (listeria monocytogenes and salmonella spp.) were monitored. starter culture b grew faster and was less inhibited by sodium lactate, thus inducting a faster and more important ph drop into the sausages. with lowe ... | 2003 | 22063699 |
| viability of listeria monocytogenes on commercially-prepared hams surface treated with acidic calcium sulfate and lauric arginate and stored at 4°c. | we demonstrated the effectiveness of delivering an antimicrobial purge/fluid into shrink-wrap bags immediately prior to introducing the product and vacuum sealing, namely the "sprayed lethality in container" (slic™) intervention delivery method. the pathogen was listeria monocytogenes, the antimicrobials were acidic calcium sulfate (acs; calcium sulfate plus lactic acid; 1:1 or 1:2 in dh(2)o) and lauric arginate (lae; ethyl-n-dodecanoyl-l-arginate hydrochloride; 5% or 10% in dh(2)o), and the pro ... | 2005 | 22064055 |
| diversity of microorganisms in the environment and dry fermented sausages of small traditional french processing units. | naturally fermented sausages produced in nine traditional french processing units and their environmental surfaces were characterised by microbial and physico-chemical analyses. salmonella and staphylococcus aureus were not detected in the environment whereas listeria monocytogenes was detected in four samples. staphylococcus/kocuria, lactic acid bacteria (lab), enterobacteriaceae, pseudomonas, yeasts/moulds and enterococci contaminated the surfaces of two processing units, indicating insufficie ... | 2007 | 22064197 |
| the fate of two listeria monocytogenes serotypes in "cig kofte" at different storage temperatures. | cig kofte is a traditional turkish food prepared from minced beef, bulgur, onions, garlic and varieties of spices. it is generally consumed within a few hours. however, leftovers can be kept in refrigerator or in room temperature up to 24h until they are consumed. in this study, survival and growth of two listeria monocytogenes serotypes were investigated in cig kofte during the storage. for this purpose, the prepared samples were separately contaminated with serotypes 1/2b or 4b of l. monocytog ... | 2007 | 22064198 |
| application of electrolyzed oxidizing water to reduce listeria monocytogenes on ready-to-eat meats. | experiments were conducted to determine the effectiveness of acidic (eoa) or basic electrolyzed oxidizing (eob) water, alone or in combination, on ready-to-eat (rte) meats to reduce listeria monocytogenes (lm). frankfurters or ham surfaces were experimentally inoculated with lm and subjected to dipping or spraying treatments (25 or 4°c for up to 30 min) with eoa, eob, and other food grade compounds. lm was reduced the greatest when frankfurters were treated with eoa and dipped at 25°c for 15 min ... | 2005 | 22064233 |
| influence of oxygen exclusion and temperature on pathogenic bacteria levels and sensory characteristics of packed ostrich steaks throughout refrigerated storage. | ostrich steaks (290) were obtained from iliofibularis muscles. for microbiological and ph determinations, samples were inoculated with listeria monocytogenes nctc 11994 (80 steaks) or escherichia coli atcc 12806 (80), then air- or vacuum-packed and stored at either 4±1°c or 10±1°c. analyses were carried out on days 0, 3, 6 and 9 of storage. for sensory evaluation, samples (130) were air- or vacuum-packed and stored at 4±1°c or at 10±1°c. sensory attributes (odour, colour, drip loss, texture and ... | 2007 | 22064287 |
| the effect of nisin on l. monocytogenes in turkish fermented sausages (sucuks). | turkish fermented sausage (sucuk) is a traditional, well-known meat product in turkey. the aim of this study was to evaluate the effect of different nisin concentrations on listeria monocytogenes in experimentally contaminated sucuk. analyses were performed on at 0, 1, 3, 5, 7, 10, 15, 20, 25 and 30days for l. monocytogenes and other microbiological parameters (total mesophilic aerobic bacteria and lactic acid bacteria) and physico-chemical parameters (ph, a(w) and moisture content).the sucuk do ... | 2007 | 22064303 |
| Deciphering the biodiversity of Listeria monocytogenes lineage III strains by polyphasic approaches. | Listeria monocytogenes is a foodborne pathogen of humans and animals. The majority of human listeriosis cases are caused by strains of lineages I and II, while lineage III strains are rare and seldom implicated in human listeriosis. We revealed by 16S rRNA sequencing the special evolutionary status of L. monocytogenes lineage III, which falls between lineages I and II strains of L. monocytogenes and the non-pathogenic species L. innocua and L. marthii in the dendrogram. Thirteen lineage III stra ... | 2011 | 22068492 |
| in vitro anti-listerial activities of crude n-hexane and aqueous extracts of garcinia kola (heckel) seeds. | we assessed the anti-listerial activities of crude n-hexane and aqueous extracts of garcinia kola seeds against a panel of 42 listeria isolates previously isolated from wastewater effluents in the eastern cape province of south africa and belonging to listeria monocytogenes, listeria grayi and listeria ivanovii species. the n-hexane fraction was active against 45% of the test bacteria with zones of inhibition ranging between 8-17 mm, while the aqueous fraction was active against 29% with zones o ... | 2011 | 22072929 |
| the pore-forming toxin listeriolysin o mediates a novel entry pathway of l. monocytogenes into human hepatocytes. | intracellular pathogens have evolved diverse strategies to invade and survive within host cells. among the most studied facultative intracellular pathogens, listeria monocytogenes is known to express two invasins-inla and inlb-that induce bacterial internalization into nonphagocytic cells. the pore-forming toxin listeriolysin o (llo) facilitates bacterial escape from the internalization vesicle into the cytoplasm, where bacteria divide and undergo cell-to-cell spreading via actin-based motility. ... | 2011 | 22072970 |
| a lysm and sh3-domain containing region of the listeria monocytogenes p60 protein stimulates accessory cells to promote activation of host nk cells. | listeria monocytogenes (lm) infection induces rapid and robust activation of host natural killer (nk) cells. here we define a region of the abundantly secreted lm endopeptidase, p60, that potently but indirectly stimulates nk cell activation in vitro and in vivo. lm expression of p60 resulted in increased ifnγ production by naïve nk cells co-cultured with treated dendritic cells (dcs). moreover, recombinant p60 protein stimulated activation of naive nk cells when co-cultured with tlr or cytokine ... | 2011 | 22072975 |
| alanine racemase mutants of mycobacterium tuberculosis require d-alanine for growth and are defective for survival in macrophages and mice. | alanine racemase (alr) is an essential enzyme in most bacteria, however some species (e.g. l. monocytogenes) can utilise d-amino acid transaminase (dat) for generating d-alanine, which renders alr non-essential. in addition to the conflicting reports on gene knockout of alr in m. smegmatis, a recent study concluded that depletion of alr does not affect the growth of m. smegmatis. in order to get an unambiguous answer on the essentiality of alr in m. tuberculosis and validate it as a drug target ... | 2011 | 22075031 |
| Listeria and autophagy escape: Involvement of InlK, an internalin-like protein. | Autophagy is a cell-autonomous mechanism of innate immunity that protects the cytosol against bacterial infection. Invasive bacteria, including Listeria monocytogenes, have thus evolved strategies to counteract a process that limits their intracellular growth. ActA is a surface protein produced by L. monocytogenes to polymerize actin and mediate intra- and intercellular movements, which plays a critical role in autophagy escape. We have recently investigated the role of another L. monocytogenes ... | 2012 | 22082958 |
| oral infection with signature-tagged listeria monocytogenes reveals organ-specific growth and dissemination routes in guinea pigs. | listeria monocytogenes causes a serious food-borne disease due to its ability to spread from the intestine to other organs, a process that is poorly understood. in this study we used 20 signature-tagged wild-type clones of l. monocytogenes in guinea pigs in combination with extensive quantitative data analysis to gain insight into extraintestinal dissemination. we show that l. monocytogenes colonized the liver in all asymptomatic animals. spread to the liver occurred as early as 4 h after ingest ... | 2011 | 22083714 |
| thiamine plays a critical role in the acid tolerance of listeria monocytogenes. | understanding the molecular basis of acid tolerance in the food-borne pathogen listeria monocytogenes is important as this property contributes to survival in the food-chain and enhances survival within infected hosts. the aim of this study was to identify genes contributing to acid tolerance in l. monocytogenes using transposon mutagenesis and subsequently to elucidate the physiological role of these genes in acid tolerance. one mutant harboring a tn917 insertion in the thit gene (formerly lmo1 ... | 2012 | 22092299 |
| Listeria monocytogenes tyrosine phosphatases affect wall teichoic acid composition and phage resistance. | Tyrosine phosphatase (PTP)-like proteins exist in many bacteria and are segregated into two major groups: low molecular weight and conventional. The latter group also has activity as phosphoinositide phosphatases. These two kinds of PTP are suggested to be involved in many aspects of bacterial physiology including stress response, DNA binding proteins, virulence, and capsule/cell wall production. By annotation, Listeria monocytogenes possesses two potential low molecular weight and two conventio ... | 2012 | 22092439 |
| listeria monocytogenes adapts to long-term stationary phase survival without compromising bacterial virulence. | bacteria withstand starvation during long-term stationary phase through the acquisition of mutations that increase bacterial fitness. the evolution of the growth advantage in stationary phase (gasp) phenotype results in the ability of bacteria from an aged culture to outcompete bacteria from a younger culture when the two are mixed together. the gasp phenotype was first described for escherichia coli, but has not been examined for an environmental bacterial pathogen, which must balance long-term ... | 2011 | 22092717 |
| [Bacteriological quality of traditional, organic and hydroponic cultured lettuce in Costa Rica]. | The main objective of this work was to evaluate the microbiological quality of lettuces commercialized in the Metropolitan Area of San José, Costa Rica, and cultured in different ways, in order to detect differences between the culturing methods and the risk that these products may represent for Public Health. The study was done at the Food Microbiology Laboratory, Universidad de Costa Rica, from March to July, 2010. 30 lettuce samples were analyzed (10 obtained by traditional culture, 10 by org ... | 2011 | 22097292 |
| visualisation of morphological interaction of diamond and silver nanoparticles with salmonella enteritidis and listeria monocytogenes. | currently, medicine intensively searches for methods to transport drugs to a target (sick) point within the body. the objective of the present investigation was to evaluate morphological characteristics of the assembles of silver or diamond nanoparticles with salmonella enteritidis (g-) or listeria monocytogenes (g+), to reveal possibilities of constructing nanoparticle-bacteria vehicles. diamond nanoparticles (nano-d) were produced by the detonation method. hydrocolloids of silver nanoparticles ... | 2011 | 22097468 |
| A strange case of waitress headache. | 2011 | 22098855 | |
| listeria monocytogenes-associated joint and bone infections: a study of 43 consecutive cases. | background. little is known about listeria monocytogenes-associated bone and joint infections. only case reports of this infection have been published. methods. retrospective study of culture-proven bone and joint cases reported to the french national reference center for listeria from 1992 to 2010. results. forty-three patients were studied: 61% were men, and the median age was 72 (range, 16-89); 24 patients exhibited comorbidities (56%). thirty-six patients (84%) had orthopedic implant devices ... | 2012 | 22100574 |
| Listeria monocytogenes cell wall constituents' charge effect on electroporation threshold. | Genetically engineered cells with mutations of relevance to electroporation, cell membrane permeabilization by electric pulses, can become a promising new tool for fundamental research on this important biotechnology. Listeria monocytogenes mutants lacking DltA or MprF and assayed for sensitivity to the cathelicidin like anti-microbial cationic peptide (mCRAMP), were developed to study the effect of cell wall charge on electroporation. Working in the irreversible electroporation regime (IRE), we ... | 2011 | 22100748 |
| purification, crystallization and preliminary x-ray crystallographic analysis of pbp4 from listeria monocytogenes. | penicillin-binding proteins (pbps), which catalyze peptidoglycan synthesis, have been extensively studied as a well established target of antimicrobial agents, including β-lactam derivatives. however, remarkable resistance to β-lactams has developed among pathogenic bacteria since the clinical use of penicillin began. recently, the glycosyltransferase (gt) domain of class a pbps has been proposed as an attractive target for antibiotic development as moenomycin-bound gt-domain structures have bee ... | 2011 | 22102039 |
| technological characterization of bacteriocin producing lactococcus lactis strains employed to control listeria monocytogenes in cottage cheese. | in recent years, there has been a particular focus on the application of antimicrobial compounds produced by lactic acid bacteria (lab) as natural preservatives to control the growth of spoilage and pathogenic bacteria in food. bacteriocins are antimicrobial peptides which can be added to foods in concentrated forms as food preservatives, e.g. additives, or they can be produced in situ by starters or protective cultures. in this study, twenty lactococcus lactis bacteriocin producers previously i ... | 2011 | 22104121 |
| Listeria monocytogenes inhibition by defatted mustard meal-based edible films. | An antimicrobial edible film was developed from defatted mustard meal (Sinapis alba) (DMM), a byproduct from the bio-fuel industry, without incorporating external antimicrobials and its antimicrobial activity against Listeria monocytogenes and physical properties were investigated. The DMM colloidal solution consisting of 184g water, 14g DMM, and 2g glycerol was homogenized and incubated at 37°C for 0.2, 0.5, 24 or 48h to prepare a film-forming solution. The pH of a portion of the film-forming s ... | 2011 | 22104123 |
| purification and characterization of enterocin mc13 produced by a potential aquaculture probiont enterococcus faecium mc13 isolated from the gut of mugil cephalus. | a bacteriocin producer strain mc13 was isolated from the gut of mugil cephalus (grey mullet) and identified as enterococcus faecium. the bacteriocin of e. faecium mc13 was purified to homogeneity, as confirmed by tricine sodium dodecyl sulphate - polyacrylamide gel electrophoresis (sds-page). reverse-phase high-performance liquid chromatography (hplc) analysis showed a single active fraction eluted at 26 min, and matrix-assisted laser desorption ionization time of flight (maldi-tof) mass spectro ... | 2011 | 22112158 |
| illuminating the landscape of host-pathogen interactions with the bacterium listeria monocytogenes. | listeria monocytogenes has, in 25 y, become a model in infection biology. through the analysis of both its saprophytic life and infectious process, new concepts in microbiology, cell biology, and pathogenesis have been discovered. this review will update our knowledge on this intracellular pathogen and highlight the most recent breakthroughs. promising areas of investigation such as the increasingly recognized relevance for the infectious process, of rna-mediated regulations in the bacterium, an ... | 2011 | 22114192 |
| listeria monocytogenes infection in macrophages induces vacuolar-dependent host mirna response. | listeria monocytogenes is a gram-positive facultative intracellular pathogen, causing serious illness in immunocompromised individuals and pregnant women. upon detection by macrophages, which are key players of the innate immune response against infection, l. monocytogenes induces specific host cell responses which need to be tightly controlled at transcriptional and post-transcriptional levels. here, we ask whether and how host mirnas, which represent an important mechanism of post-transcriptio ... | 2011 | 22114673 |
| Pulsed-field gel electrophoresis (PFGE) analysis of Listeria monocytogenes isolates of clinical, animal, food, and environmental origin from Ireland. | Listeria monocytogenes is an important foodborne human pathogen. Human infection is associated with high mortality rates. Epidemiological investigation and molecular sub-typing can be useful in linking specific sources of infection with human illness. This study describes retrospective use of pulsed-field gel electrophoresis (PFGE) to examine relationships of 222 isolates from human and non-human sources in Ireland. Human clinical isolates from other countries were also examined. Eight small cl ... | 2011 | 22116984 |
| transfer of foodborne pathogenic bacteria to non-inoculated beef fillets through meat mincing machine. | the aim of this study was to evaluate the transfer of pathogens population to non-inoculated beef fillets through meat mincing machine. in this regard, cocktails of mixed strain cultures of each listeria monocytogenes, salmonella enterica ser. typhimurium and escherichia coli o157:h7 were used for the inoculation of beef fillets. three different initial inoculum sizes (3, 5, or 7 log cfu/g) were tested. the inoculated beef fillets passed through meat mincing machine and then, six non-inoculated ... | 2012 | 22119672 |
| identification of genes involved in listeria monocytogenes biofilm formation by mariner-based transposon mutagenesis. | listeria monocytogenes is a ubiquitous food-borne pathogen, whose distribution and survival in food-processing environments are associated with the ability to form biofilms. the process of biofilm formation is complex and its molecular mechanism is relatively poorly understood in l. monocytogenes. to better understand the genetics of this process, a mariner-based transposon mutagenesis strategy was used to identify genes involved in biofilm formation of l. monocytogenes. a library of 6,500 mutan ... | 2011 | 22120623 |
| HTLV-1 bZIP factor impairs cell-mediated immunity by suppressing production of Th1 cytokines. | Adult T-cell leukemia (ATL) patients and human T-cell leukemia virus-1 (HTLV-1) infected individuals succumb to opportunistic infections. Cell mediated immunity is impaired, yet the mechanism of this impairment has remained elusive. The HTLV-1 basic leucine zipper factor (HBZ) gene is encoded in the minus strand of the viral DNA and is constitutively expressed in infected cells and ATL cells. To test the hypothesis that HBZ contributes to HTLV-1 associated immunodeficiency, we challenged transge ... | 2011 | 22123848 |
| cytotoxicity of listeriolysin o produced by membrane-encapsulated bacillus subtilis on leukemia cells. | encapsulation of biological material in the permiselective membrane allows to construct a system separating cells from their products, which may find biotechnological as well as biomedical applications in biological processes regulation. application of a permiselective membrane allows avoiding an attack of the implanted microorganisms on the host. our aim was to evaluate the performance of bacillus subtilis encapsulated in an elaborate membrane system producing listeriolysin o, a cytolysin from ... | 2011 | 22127132 |
| the listeria monocytogenes σb regulon and its virulence-associated functions are inhibited by a small molecule. | the stress-responsive alternative sigma factor σ(b) is conserved across diverse gram-positive bacterial genera. in listeria monocytogenes, σ(b) regulates transcription of >150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such as those encountered in the human gastrointestinal lumen. an inhibitor of l. monocytogenes σ(b) activity was identified by screening ~57,000 natural and synthesized small molecules using a high-throughpu ... | 2011 | 22128349 |
| phage and their lysins as biocontrol agents for food safety applications. | bacteriophage (phage) are bacterial viruses and are considered to be the most widely distributed and diverse natural biological entities. soon after their discovery, bacteriophage were found to have antimicrobial properties that were exploited in many early anti-infection trials. however, the subsequent discovery of antibiotics led to a decline in the popularity of bacteriophage in much of the western world, although work continued in the former soviet union and eastern europe. as a result of th ... | 2010 | 22129344 |
| inhibitory effects of uv treatment and a combination of uv and dry heat against pathogens on stainless steel and polypropylene surfaces. | pathogens that contaminate the surfaces of food utensils may contribute to the occurrence of foodborne disease outbreaks. we investigated the efficacy of uv treatment combined with dry heat (50 °c) for inhibiting 5 foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, pseudomonas aeruginosa, listeria monocytogenes, and staphylococcus aureus) on stainless steel and polypropylene surfaces in this study. we inoculated substrates with each of the 5 foodborne pathogens cultured on ag ... | 2011 | 22132742 |
| effect of packaging and storage time on survival of listeria monocytogenes on kippered beef steak and turkey tenders. | the objective of our study was to determine effect of packaging method and storage time on reducing listeria monocytogenes in shelf-stable meat snacks. commercially available kippered beef steak strips and turkey tenders were dipped into a 5-strain l. monocytogenes cocktail, and dried at 23 °c until a water activity of 0.80 was achieved. inoculated samples were packaged with 4 treatments: (1) vacuum, (2) nitrogen flushed with oxygen scavenger, (3) heat sealed with oxygen scavenger, and (4) heat ... | 2011 | 22133033 |
| Quality Parameters and Antioxidant and Antibacterial Properties of Some Mexican Honeys. | A total of 14 Mexican honeys were screened for quality parameters including color, moisture, proline, and acidity. Antioxidant properties of complete honey and its methanolic extracts were evaluated by the DPPH, ABTS, and FRAP assays. In addition, the antimicrobial activity of complete honeys against Bacillus cereus ATCC 10876, Listeria monocytogenes Scott A, Salmonella Typhimurium ATCC 14028, and Sthapylococcus aureus ATCC 6538 was determined. Most of honeys analyzed showed values within qual ... | 2011 | 22133067 |
| Investigation of the role of ZurR in the physiology and pathogenesis of Listeria monocytogenes. | Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocatalysts in cellular processes. The zinc uptake regulator (ZurR) is predicted to coordinate uptake of zinc from the external environment. An in-frame deletion of the zurR gene resulted i ... | 2011 | 22133190 |
| atypical listeria monocytogenes serotype 4b strains harboring a lineage ii-specific gene cassette. | listeria monocytogenes is the etiological agent of listeriosis, a severe food-borne illness. the population of l. monocytogenes is divided into four lineages (i to iv), and serotype 4b in lineage i has been involved in numerous outbreaks. several serotype 4b epidemic-associated clonal groups (eci, -ii, and -ia) have been identified. in this study, we characterized a panel of strains of serotype 4b that produced atypical results with a serotype-specific multiplex pcr and possessed the lmo0734 to ... | 2011 | 22138999 |
| sigb-dependent tolerance to protein synthesis-inhibiting antibiotics in listeria monocytogenes egde. | the alternative sigma factor sigb in food-borne pathogen listeria monocytogenes was determined in this study to be required for tolerance to protein synthesis-inhibiting antibiotics. the minimum inhibitory concentrations of tetracycline hcl and gentamicin sulphate against egdeδsigb were two- and fourfold less than those for egde, respectively. the ability of egdeδsigb to overcome the growth arrest caused by erythromycin and rifampin was also weaker than that of egde. the transcription analysis o ... | 2011 | 22143398 |
| bacteriocin production and resistance to drugs are advantageous features for lactobacillus acidophilus la-14, a potential probiotic strain. | l. acidophilus la-14 produces bacteriocin active against l. monocytogenes scotta (1600 au/ml) in mrs broth at 30°c or 37°c. the bacteriocin proved inhibitory to different serological types of listeria spp. antimicrobial activity was completely lost after treatment of the cell-free supernatant with proteolytic enzymes. addition of bacteriocin produced by l. acidophilus la-14 to a 3 h-old culture of l. monocytogenes scotta repressed cell growth in the following 8h. treatment of stationary phase ce ... | 2011 | 22143809 |
| Characterization of an anti-listerial enterocin from wheat silage based Enterococcus faecium. | Two Enterococcus faecium and one E. faecalis strains isolated and identified from wheat silage were characterized based on plasmid content, hemolytic activity, antibiotic resistance patterns, bacteriocin production potential, and presence of enterocin structural genes (entA, entB, entP, entL50B). Among the isolates, only the E. faecium U7 strain exhibited bacteriocin activity against Listeria monocytogenes ATCC 7644, and vancomycin resistant Enterococcus spp. (VRE). A combination of three struct ... | 2011 | 22144257 |
| Molecular Techniques in Ecohealth Research Toolkit: Facilitating Estimation of Aggregate Gastroenteritis Burden in an Irrigated Periurban Landscape. | Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques i ... | 2011 | 22146856 |
| microbial infection-induced expansion of effector t cells overcomes the suppressive effects of regulatory t cells via an il-2 deprivation mechanism. | regulatory foxp3(+) t cells are a critical cell population that suppresses t cell activation in response to microbial and viral pathogens. we identify a cell-intrinsic mechanism by which effector cd4(+) t cells overcome the suppressive effects of regulatory t (treg) cells in the context of three distinct infections: toxoplasma gondii, listeria monocytogenes, and vaccinia virus. the acute responses to the parasitic, bacterial, and viral pathogens resulted in a transient reduction in frequency and ... | 2011 | 22147768 |
| a live-attenuated listeria vaccine (anz-100) and a live-attenuated listeria vaccine expressing mesothelin (crs-207) for advanced cancers: phase i studies of safety and immune induction. | purpose: listeria monocytogenes (lm)-based vaccines stimulate both innate and adaptive immunity. anz-100 is a live-attenuated lm strain (lm δacta/δinlb). uptake by phagocytes in the liver results in local inflammatory responses and activation and recruitment of natural killer (nk) and t cells, in association with increased survival of mice bearing hepatic metastases. the lm δacta/δinlb strain, engineered to express human mesothelin (crs-207), a tumor-associated antigen expressed by a variety of ... | 2011 | 22147941 |
| Cold Plasma Decontamination of Foods. | Cold plasma is a novel nonthermal food processing technology that uses energetic, reactive gases to inactivate contaminating microbes on meats, poultry, fruits, and vegetables. This flexible sanitizing method uses electricity and a carrier gas, such as air, oxygen, nitrogen, or helium; antimicrobial chemical agents are not required. The primary modes of action are due to UV light and reactive chemical products of the cold plasma ionization process. A wide array of cold plasma systems that operat ... | 2011 | 22149075 |
| the survival of e. coli o157:h7, s. typhimurium and l. monocytogenes in black carrot (daucus carota) juice. | in this study the survival and growth patterns of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in various concentrations of black carrot juice were investigated during incubation period at 4°c and 37°c for 7days. several parameters, such as juice concentration (%), ph, incubation temperature (°c) and time (days) were found effective on the survival of pathogens tested. although l. monocytogenes has been found to be the less resistant microorganism to the variable c ... | 2011 | 22153385 |
| [listeria monocytogenes osteomyelitis.] | 2011 | 22153782 | |
| transcription of pattern recognition receptors and abortive agents induced chemokines in the bovine pregnant uterus. | pattern recognition receptors (prrs) are important components of the innate immune system whose ligands are specific pathogen associated molecular patterns (pamps). considering the scarcity of studies on transcription of prrs in the pregnant uterus of cows, and its response to pamps and microorganisms that cause abortion in cattle, this study aimed to characterize the transcription of tlr1-10, nod1, nod2 and md2 in bovine uterus throughout gestation and to investigate the sensitivity of differen ... | 2011 | 22153993 |
| Nano-magnetic primer based electrochemiluminescence-polymerase chain reaction (NMPE-PCR) assay. | Here we have developed a novel nano-magnetic primer based electrochemiluminescence-polymerase chain reaction (NMPE-PCR) strategy for detection of genome. The key idea of this method is integrating the two in situ processes: PCR on the surface of magnetic nanoparticles (MNPs) and magnetic beads based ECL readout platform, to avoid some laborious manual operations and achieve rapid yet sensitive detection. At first, the approach employs a pair of functional primers for amplification: one is tris-( ... | 2012 | 22154403 |
| establishing equivalence for microbial growth inhibitory effects ("iso-hurdle rules") by analysing disparate data on listeria monocytogenes with a gamma-type predictive model. | preservative factors act as hurdles against microorganisms by inhibiting their growth; these are essential control measures for particular foodborne pathogens. different combinations of hurdles can be quantified and compared to each other in terms of their inhibitory effect ("iso-hurdle"). this paper presents a methodology for establishing microbial iso-hurdle rules in three steps: developing a predictive model based on existing but disparate datasets; building an experimental design focused on ... | 2011 | 22156426 |
| Identification of components of the host type IA PI 3-kinase pathway that promote internalization of Listeria monocytogenes. | The bacterial pathogen Listeria monocytogenes causes food-borne illnesses resulting in gastroenteritis, meningitis, or abortion. Listeria promotes its internalization into some human cells through binding of the bacterial surface protein InlB to the host receptor tyrosine kinase Met. Interaction of InlB with the Met receptor stimulates host signaling pathways that promote cell surface changes driving bacterial uptake. One human signaling protein that plays a critical role in Listeria entry is ty ... | 2011 | 22158742 |
| characterization and expression of attacin, an antibacterial protein-encoding gene, from the beet armyworm, spodoptera exigua (hübner) (insecta: lepidoptera: noctuidae). | to isolate antimicrobial-related genes from the beet armyworm, spodoptera exigua, we performed genefishing, a polymerase chain reaction (pcr)-based differential display technique. an attacin-like complementary dna (cdna) including a 3'-untranslated region was identified from among 18 over-expressed genes in microbial-infected larvae. the full-length attacin cdna from s. exigua cdna (seattacin) was cloned using rapid amplification of cdna ends pcr. the attacin-like cdna transcript was 765 nucleot ... | 2011 | 22160467 |
| perforin plays an unexpected role in regulating t-cell contraction during prolonged listeria monocytogenes infection. | after infection or vaccination, antigen-specific t cells proliferate then contract in numbers to a memory set point. t-cell contraction is observed after both acute and prolonged infections although it is unknown if contraction is regulated similarly in both scenarios. here, we show that contraction of antigen-specific cd8(+) and cd4(+) t cells is markedly reduced in tnf/perforin-double deficient (dko) mice responding to attenuated listeria monocytogenes infection. reduced contraction in dko m ... | 2011 | 22161269 |
| generation of variants in listeria monocytogenes continuous-flow biofilms is dependent on radical-induced dna damage and reca-mediated repair. | the food-borne pathogen listeria monocytogenes is a gram-positive microaerophilic facultative anaerobic rod and the causative agent of the devastating disease listeriosis. l. monocytogenes is able to form biofilms in the food processing environment. since biofilms are generally hard to eradicate, they can function as a source for food contamination. in several occasions biofilms have been identified as a source for genetic variability, which potentially can result in adaptation of strains to foo ... | 2011 | 22163039 |
| Phage-based biocontrol strategies to reduce foodborne pathogens in foods. | There has been much recent interest in the use of phages as biocontrol agents of foodborne pathogens in animals used for food production, and in the food products themselves. This interest seems to be driven by consumers' request for more natural foods, as well as the fact that foodborne outbreaks continue to occur, globally, in many foods, some of which (such as fresh produce), lack adequate methods to control any pathogenic contamination present. Also, the many successes with respect to regula ... | 2011 | 22164346 |
| Reporter bacteriophage A511::celB transduces a hyperthermostable glycosidase from Pyrococcus furiosus for rapid and simple detection of viable Listeria cells. | Reporter bacteriophages for detection of pathogenic bacteria offer fast and sensitive screening for live bacterial targets. We present a novel strategy employing a gene encoding a hyperthermophilic enzyme, permitting the use of various substrates and assay formats. The celB gene from the hyperthermophilic archaeon Pyrococcus furiosus specifying an extremely thermostable ß-glycosidase was inserted into the genome of the broad host range, virulent Listeria phage A511 by homologous recombination. I ... | 2011 | 22164348 |
| Evaluation of applied biosystems MicroSeq real-time PCR system for detection of Listeria monocytogenes in food. Performance Tested Method 011002. | Increasingly, more food companies are relying on molecular methods, such as PCR, for pathogen detection due to their improved simplicity, sensitivity, and rapid time to results. This report describes the validation of a new Real-Time PCR method to detect Listeria monocytogenes in nine different food matrixes. The complete system consists of the MicroSEQ L. monocytogenes Detection Kit, sample preparation, the Applied Biosystems 7500 Fast Real-Time PCR instrument, and RapidFinder Express software. ... | 2011 | 22165012 |