Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| use of nanoparticulate zinc oxide as intracanal medication in endodontics: ph and antimicrobial activity. | the aim of this study was to evaluate the ph and antimicrobial activity of micro or nanoparticulate zinc oxide (zno) pastes with or without calcium hydroxide (ch). the following medications were evaluated: microparticulate zno + polyethylene glycol (peg) 400; nanoparticulate zno + peg 400; peg 400; ch + microparticulate zno + peg 400 and ch + nanoparticulate zno + peg 400. the ph was assessed between 12 hours and 28 days, using a digital ph meter. the antimicrobial activity against enterococcus ... | 2013 | 25335366 |
| engineering acetyl coenzyme a supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of saccharomyces cerevisiae. | the energetic (atp) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. cytosolic acetyl coenzyme a (acetyl-coa) is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into saccharomyces cerevisiae, such as isoprenoids or lipids. in this yeast, synthesis of cytosolic acetyl-coa via acetyl-coa synthetase (acs) involves hydrolysis of atp to amp and pyrophosphat ... | 2014 | 25336454 |
| clinical epidemiological study on the incidence of escherichia coli infections in the cancer patients admitted to surgery department ii of the iasi regional oncology institute in 2013. | the aim of study was to identify pathogens involved in hospital-acquired or community-acquired infections in patients suffering from various types of cancers. | 2014 | 25341303 |
| first report of new delhi metallo-β-lactamase-1-producing strains in slovakia. | occurrence of carbapenemase-producing organisms, including new delhi metallo-β-lactamase-1 (ndm-1) is increasingly reported worldwide. the aim of this study was to assess the distribution of carbapenemase producers among multidrug-resistant gram-negative bacteria isolated from blood cultures. all carbapenem-resistant strains collected from december 2011 to december 2012 were analyzed. presence of carbapenemases was assessed with combined disc test and carba np test followed by polymerase chain r ... | 2015 | 25343711 |
| riboflavin-shuttled extracellular electron transfer from enterococcus faecalis to electrodes in microbial fuel cells. | great attention has been focused on gram-negative bacteria in the application of microbial fuel cells. in this study, the gram-positive bacterium enterococcus faecalis was employed in microbial fuel cells. bacterial biofilms formed by e. faecalis zer6 were investigated with respect to electricity production through the riboflavin-shuttled extracellular electron transfer. trace riboflavin was shown to be essential for transferring electrons derived from the oxidation of glucose outside the peptid ... | 2014 | 25345758 |
| baseline activity of telavancin against gram-positive clinical isolates responsible for documented infections in u.s. hospitals (2011-2012) as determined by the revised susceptibility testing method. | telavancin had mic50 and mic90 values of 0.03 and 0.06 μg/ml (100.0% susceptible), respectively, against methicillin-resistant and -susceptible staphylococcus aureus. telavancin was active against vancomycin-susceptible enterococcus faecalis (mic50/90, 0.12/0.12 μg/ml; 100% susceptible) and enterococcus faecium (mic50/90, 0.03/0.06 μg/ml), while higher mic values were obtained against vancomycin-resistant e. faecium (mic50/90, 1/2 μg/ml) and e. faecalis (mic50/90, >2/>2 μg/ml). streptococci show ... | 2015 | 25348529 |
| [study of association between parvimonas micra and pulp dominant pathogens in the infected root canals with chronic periradicular periodontitis]. | to study the prevalence of parvimonas micra (pm) and the associations between pm and pulp dominant pathogens in order to reflect the colonization of pm in the infected root canals with chronic periradicular periodontitis. | 2014 | 25351224 |
| biological activities of xylooligosaccharides generated from garlic straw xylan by purified xylanase from bacillus mojavensis ueb-fk. | a newly isolated bacterium strain named ueb-fk was selected from tunisian sahara, exhibiting the highest clear zone on agar plates containing oat spelt xylan by staining with congo red. on the basis of 16s rdna sequence analysis, this strain was identified as bacillus mojavensis. this strain produced extracellular xylanase. xylanase from the strain was purified to homogeneity and had an apparent molecular weight of 14 kda. the k m and v max values of the purified xylanase on oat spelt xylan were ... | 2015 | 25351626 |
| healthcare-associated infective endocarditis: a case series in a referral hospital from 2006 to 2011. | healthcare-associated infective endocarditis (hca-ie), a severe complication of medical care, shows a growing incidence in literature. | 2014 | 25352503 |
| genomic annotation for the temperate phage efc-1, isolated from enterococcus faecalis kbl101. | the temperate phage efc-1 was newly isolated from a mitomycin-c-induced lysate of enterococcus faecalis kbl101. efc-1 has an isometric head and a long tail. the phage belongs to the family siphoviridae according to its genomic structure and morphology. the phage efc-1 has 40,286 base pairs of double-stranded dna and a g+c content of 35.05 %. bioinformatic analysis of the phage revealed 60 putative open reading frames (orfs). the genome of the temperate phage efc-1 was not significantly similar t ... | 2015 | 25359106 |
| a liar deletion restores susceptibility to daptomycin and antimicrobial peptides in multidrug-resistant enterococcus faecalis. | daptomycin is a lipopeptide antibiotic that is used clinically against many gram-positive bacterial pathogens and is considered a key frontline bactericidal antibiotic to treat multidrug-resistant enterococci. emergence of daptomycin resistance during therapy of serious enterococcal infections is a major clinical issue. in this work, we show that deletion of the gene encoding the response regulator, liar (a member of the liafsr system that controls cell envelope homeostasis), from daptomycin-res ... | 2015 | 25362197 |
| activity of oritavancin against gram-positive clinical isolates responsible for documented skin and soft-tissue infections in european and us hospitals (2010-13). | to assess oritavancin activity in vitro against clinically relevant gram-positive pathogens causing skin and soft-tissue infections (sstis) in european and us hospitals. | 2015 | 25362568 |
| effect of photodynamic therapy (pdt) on enterococcus faecalis biofilm in experimental primary and secondary endodontic infections. | to determine the antibacterial effect of photodynamic therapy on enterococcus faecalis (e. faecalis) biofilms in experimentally infected human root canals in primary infections and endodontic retreatments. | 2014 | 25366394 |
| bacteriocin protein bacl1 of enterococcus faecalis targets cell division loci and specifically recognizes l-ala2-cross-bridged peptidoglycan. | bacteriocin 41 (bac41) is produced from clinical isolates of enterococcus faecalis and consists of two extracellular proteins, bacl1 and baca. we previously reported that bacl1 protein (595 amino acids, 64.5 kda) is a bacteriolytic peptidoglycan d-isoglutamyl-l-lysine endopeptidase that induces cell lysis of e. faecalis when an accessory factor, baca, is copresent. however, the target of bacl1 remains unknown. in this study, we investigated the targeting specificity of bacl1. fluorescence micros ... | 2015 | 25368300 |
| antibacterial efficacy of aqueous ozone in root canals infected by enterococcus faecalis. | in endodontics, the elimination of resistant bacteria such as enterococcus faecalis plays an important role for treatment success in root canals. therefore, new alternative irrigants (instead of sodium hypochlorite) have been researched to achieve ideal endodontic treatment. | 2014 | 25368798 |
| identification of peptidoglycan-associated proteins as vaccine candidates for enterococcal infections. | infections by opportunistic bacteria have significant contributions to morbidity and mortality of hospitalized patients and also lead to high expenses in healthcare. in this setting, one of the major clinical problems is caused by gram-positive bacteria such as enterococci and staphylococci. in this study we extract, purify, identify and characterize immunogenic surface-exposed proteins present in the vancomycin resistant enterococci (vre) strain enterococcus faecium e155 using three different e ... | 2014 | 25369230 |
| a tir domain protein from e. faecalis attenuates myd88-mediated signaling and nf-κb activation. | toll-like receptor signaling, mediated by functional toll/interleukin-1 receptor (tir) domains, plays a critical role in activating the innate immune response responsible for controlling and clearing infection. bacterial protein mimics of components of this signaling pathway have been identified and function through inhibition of interactions between toll-like receptors (tlrs) and their adaptor proteins, mediated by tir domains. a previously uncharacterized gene, which we have named tcpf (for ti ... | 2014 | 25369374 |
| phytochemical investigation and anti-microbial activity of clausena anisata (willd), hook. | clausena anisata belongs to the family rutaceae, a shrub widely used in west africa for the treatment of bacterial and fungal infections of the skin including boils, ringworm and eczema. the study was designed to evaluate the antimicrobial activity and phytochemical screening of ethanol leaf extract of c. anisata (cle). | 2014 | 25371583 |
| in vitro evaluation of the antimicrobial efficacy of four endodontic biomaterials against enterococcus faecalis, candida albicans, and staphylococcus aureus. | root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. this study evaluated the antimicrobial activity of four endodontic sealers, ah plus, tubliseal ewt, endorez, and iroot sp, against three different microorganisms, e. faecalis, c. albicans, and s. aureus, by direct contact test. 10 μl microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°c. subsequentl ... | 2014 | 25371678 |
| aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland. | we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ... | 2015 | 25380369 |
| differential susceptibility of bacteria to mouse paneth cell α-defensins under anaerobic conditions. | small intestinal paneth cells secrete α-defensin peptides, termed cryptdins (crps) in mice, into the intestinal lumen, where they confer immunity to oral infections and define the composition of the ileal microbiota. in these studies, facultative bacteria maintained under aerobic or anaerobic conditions displayed differential sensitivities to mouse α-defensins under in vitro assay conditions. regardless of oxygenation, crps 2 and 3 had robust and similar bactericidal activities against s. typhim ... | 2014 | 25383215 |
| antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review - part i. in vitro studies. | the goal of endodontic treatment is the prevention and control of pulpal and periradicular infections. calcium hydroxide (ca(oh)2) has been widely used in endodontics as an intracanal medicament to eliminate the remaining microorganisms after chemomechanical preparation. the purpose of this article is to review the antimicrobial properties of ca(oh)2 as an intracanal medicament in root canal treatment. the first part of this review details the characteristics of ca(oh)2 and summarizes the result ... | 2014 | 25383341 |
| microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. in vitro sem study. | this in vitro study aimed to investigate the ability of candida albicans (c. albicans) and enterococcus faecalis (e. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. | 2014 | 25383343 |
| an in vivo comparison of internal bacterial colonization in two dental implant systems: identification of a pathogenic reservoir. | the aim of this study was to compare internal bacterial colonization in two implant systems, one screw root form (srf) with an external hexagon connection and one plateau root form (prf) with a morse taper internal connection. | 2015 | 25385682 |
| the two-component system grvrs (etars) regulates ace expression in enterococcus faecalis og1rf. | expression of ace (adhesin to collagen of enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46 °c. however, the mechanism of ace/ace regulation under different conditions is still unknown. in this study, we identified a two-component regulatory system grvrs as the main regulator of ace expression under these stress con ... | 2015 | 25385790 |
| the polyamine n-acetyltransferase-like enzyme pmve plays a role in the virulence of enterococcus faecalis. | we previously showed that the mutant strain of enterococcus faecalis lacking the transcriptional regulator slya is more virulent than the parental strain. we hypothesized that this phenotype was due to overexpression of the second gene of the slya operon, ef_3001, renamed pmve (for polyamine metabolism and virulence of e. faecalis). pmve shares strong homologies with n(1)-spermidine/spermine acetyltransferase enzymes involved in the metabolism of polyamines. in this study, we used an e. faecalis ... | 2015 | 25385793 |
| enterococcus faecalis 6-phosphogluconolactonase is required for both commensal and pathogenic interactions with manduca sexta. | enterococcus faecalis is a commensal and pathogen of humans and insects. in manduca sexta, e. faecalis is an infrequent member of the commensal gut community, but its translocation to the hemocoel results in a commensal-to-pathogen switch. to investigate e. faecalis factors required for commensalism, we identified e. faecalis genes that are upregulated in the gut of m. sexta using recombinase-based in vivo expression technology (rivet). the rivet screen produced 113 clones, from which we identif ... | 2015 | 25385794 |
| an in vitro comparison of the antibacterial efficacy of triphala with different concentrations of sodium hypochlorite. | the antimicrobial efficacy of root canal irrigant plays an important role in increasing the success of root canal treatment (rct). the aim of the present experimental study was to compare the antimicrobial activity of triphala (a plant-derived solution) with 0.5, 1, 2.5 and 5% concentrations of sodium hypochlorite (naocl), against enterococcus faecalis (e. faecalis). | 2014 | 25386211 |
| comparative prevalence of antimicrobial resistance in community-acquired urinary tract infection cases from representative states of northern and southern india. | urinary tract infections (utis) are amongst the most common infections described in outpatient settings. increased antimicrobial resistance (amr) of urinary tract pathogens is a matter of global public health concern. treatment of uti depends on both prevalence and antimicrobial resistance (amr) of causative bacteria at any specific geographical location. | 2014 | 25386432 |
| estimation of cultivable bacterial diversity in the cloacae and pharynx in eurasian griffon vultures (gyps fulvus). | in this work, we describe the biodiversity of cloacal and pharynx culture-based bacteria (commensal and pathogenic), in 75 eurasian griffon vultures (gyps fulvus) from two geographic areas. we address the question of whether the cultivable microbiota of vultures is organised into assemblages occurring by chance. in addition, we assess bacterial diversity in both anatomic regions and geographic areas. bacterial diversity was represented by 26 gram-negative and 20 gram-positive genera. the most co ... | 2015 | 25388757 |
| macular infarction after enterococcus faecalis endophthalmitis. | to describe a case of macular infarction occurring after enterococcus faecalis endophthalmitis in a patient who had undergone cataract surgery. | 2009 | 25389576 |
| australian enterococcal sepsis outcome progamme, 2011. | from 1 january to 31 december 2011, 29 institutions around australia participated in the australian enterococcal sepsis outcome programme (aesop). the aim of aesop 2011 was to determine the proportion of enterococcal bacteraemia isolates in australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterise the molecular epidemiology of the enterococcus faecalis and e. faecium isolates. of the 1,079 unique episodes of ba ... | 2014 | 25391408 |
| anti-bacterial and anti-oxidant activities of leaf extracts of combretum vendae (combretecacea) and the isolation of an anti-bacterial compound. | combretum vendae a.e. van wyk (combretaceae) is used for the treatment of bacterial related infections and oxidative related diseases by indigenous people of south africa. dried leaves extracts of c. vendae were investigated for bioactivity against a variety of bacterial strains and their antioxidant potential evaluated. | 2014 | 25395708 |
| role of secondary metabolites in establishment of the mutualistic partnership between xenorhabdus nematophila and the entomopathogenic nematode steinernema carpocapsae. | xenorhabdus nematophila engages in a mutualistic partnership with the nematode steinernema carpocapsae, which invades insects, migrates through the gut, and penetrates into the hemocoel (body cavity). we showed previously that during invasion of manduca sexta, the gut microbe staphylococcus saprophyticus appeared transiently in the hemocoel, while enterococcus faecalis proliferated as x. nematophila became dominant. x. nematophila produces diverse secondary metabolites, including the major water ... | 2015 | 25398871 |
| molecular characterization and antibiotic resistance of enterococcus species from gut microbiota of chilean altiplano camelids. | enterococcus is one of the major human pathogens able to acquire multiple antibiotic-resistant markers as well as virulence factors which also colonize remote ecosystems, including wild animals. in this work, we characterized the enterococcus population colonizing the gut of chilean altiplano camelids without foreign human contact. | 2014 | 25405007 |
| effects of adsorbed and templated nanosilver in mesoporous calcium-silicate nanoparticles on inhibition of bacteria colonization of dentin. | mesoporous calcium-silicate nanoparticles (mcsns) are advanced biomaterials for controlled drug delivery and mineralization induction. nanosilver-incorporated mcsns (ag-mcsns) were prepared in the present study using both the adsorption and template methods. both versions of ag-mcsns showed characteristic morphology of mesoporous materials and exhibited sustained release of ions over time. in antibacterial testing against planktonic enterococcus faecalis, ag-mcsns showed significantly better ant ... | 2014 | 25419127 |
| mechanisms of linezolid resistance in staphylococci and enterococci isolated from two teaching hospitals in shanghai, china. | linezolid is one of the most effective treatments against gram-positive pathogens. however, linezolid-resistant/intermediate strains have recently emerged in worldwide. the purpose of this study was to analyse the prevalence and resistance mechanisms of linezolid-resistant/intermediate staphylococci and enterococci in shanghai, china. | 2014 | 25420718 |
| blood stream infections in renal transplant recipients: a single-center study. | bacteremias among renal transplant recipients are more frequent as a result of immunosuppression. they are considered extremely high-risk because they are correlated with decreased allograft and recipient survival. | 2014 | 25420856 |
| definitive structural assessment of enterococcal diheteroglycan. | enterococcus faecalis is one of most important nosocomial and often multi-antibiotic resistant pathogens responsible for infections that are difficult to treat. previously, a cell-wall polysaccharide termed diheteroglycan (dhg) was isolated and characterized as a promising vaccine candidate. however, the configuration of its lactic acid (la) residue attached to the galactofuranoside unit was not assessed, although it influences conformation of dhg chain in terms of biological recognition and imm ... | 2015 | 25421964 |
| mechanical reduction of the intracanal enterococcus faecalis population by hyflex cm, k3xf, protaper next, and two manual instrument systems: an in vitro comparative study. | in the present study, the effectiveness of three rotary and two manual nickel titanium instrument systems on mechanical reduction of the intracanal enterococcus faecalis population was evaluated. | 2016 | 25424649 |
| comparative structural and functional analysis of staphylococcus aureus glucokinase with other bacterial glucokinases. | glucokinase is classified in bacteria based upon having atp binding site and 'repressor/open reading frames of unknown function/sugar kinases' motif, the sequence of glucokinase gene (jn645812) of staphylococcus aureus atcc12600 showed presence of atp binding site and 'repressor/open reading frames of unknown function/sugar kinases' motif. we have earlier observed glucokinase of s. aureus has higher affinity towards the substrate compared to other bacterial glucokinase and under anaerobic condit ... | 2014 | 25425757 |
| bacterial leakage in root canals filled with resin-based and mineral trioxide aggregate-based sealers. | sealing ability is one of the most important features of endodontic sealers. the purpose of this in vitro study was to compare the sealing ability of a resin-based sealer with a mineral trioxide aggregate-based sealer. | 2014 | 25426153 |
| in vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens. | we compared different immunoglobulin preparations containing igg (intraglobin/intratect) or a mixture of igg, iga, and igm (pentaglobin) to assess the opsonic and protective efficacy of human immunoglobulin preparations against multiresistent nosocomial pathogens. | 2015 | 25428225 |
| in vitro activity of heather [calluna vulgaris (l.) hull] extracts on selected urinary tract pathogens. | calluna vulgaris l. hull (ericaceae) has been used for treatment of urinary tract infections in traditional medicine. in this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of escherichia coli, enterococcus faecalis and proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) were determined. the concentrations of total phenols ... | 2014 | 25428676 |
| antimicrobial bioassay-guided fractionation of a methanol extract of eupatorium triplinerve. | eupatorium triplinerve vahl (asteraceae), popularly known as japana, is widely used in folk medicine, due its analgesic, anticoagulant, antianorexic, antiparasitic, anthelmintic, sedative, antifungal, and antibacterial properties. | 2015 | 25430540 |
| enterococcus faecalis pcf10-encoded surface proteins prga, prgb (aggregation substance) and prgc contribute to plasmid transfer, biofilm formation and virulence. | enterococcus faecalis pcf10 transfers at high frequencies upon pheromone induction of the prgq transfer operon. this operon codes for three cell wall-anchored proteins - prga, prgb (aggregation substance) and prgc - and a type iv secretion system through which the plasmid is delivered to recipient cells. here, we defined the contributions of the prg surface proteins to plasmid transfer, biofilm formation and virulence using the caenorhabditis elegans infection model. we report that a combination ... | 2015 | 25431047 |
| effect of zirconium oxide and zinc oxide nanoparticles on physicochemical properties and antibiofilm activity of a calcium silicate-based material. | the aim of the present study was to evaluate the antibiofilm activity against enterococcus faecalis, compressive strength. and radiopacity of portland cement (pc) added to zirconium oxide (zro2), as radiopacifier, with or without nanoparticulated zinc oxide (zno). the following experimental materials were evaluated: pc, pc + zro2, pc + zro2 + zno (5%), and pc + zro2 + zno (10%). antibiofilm activity was analyzed by using direct contact test (dct) on enterococcus faecalis biofilm, for 5 h or 15 h ... | 2014 | 25431798 |
| pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea. | linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea. | 2015 | 25433250 |
| in-vitro screening of malaysian honey from different floral sources for antibacterial activity on human pathogenic bacteria. | different researches on therapeutic effects of honey have been conducted in different regions; however the study on the potential antibacterial activity of malaysian honey is still limited. in this study, antibacterial activities of different monofloral honey samples were tested against several common human pathogenic bacteria. | 2014 | 25435614 |
| molecular recognition of live methicillin-resistant staphylococcus aureus cells using dna aptamers. | to generate dna-aptamers binding to methicillin-resistant staphylococcus aureus (mrsa). | 2013 | 25436184 |
| the question of pathogen quantification in disinfected graywater. | graywater (gw) reuse for irrigation is recognized as a sustainable solution for water conservation. one of the major impediments to gw reuse is the presence of pathogenic microorganisms. this study monitored three similar on-site gw treatment systems bi-monthly over the course of a year to compare the presence of pathogens and indicators in raw, biologically treated, and biologically treated and disinfected [by chlorine and ultraviolet light (uv)] gw. the systems were designed to allow the testi ... | 2015 | 25437766 |
| identification of enterococcus faecium and enterococcus faecalis as vanc-type vancomycin-resistant enterococci (vre) from sewage and river water in the provincial city of miyazaki, japan. | as a first step for assessing the risk to human health posed by vancomycin-resistant enterococci (vre) in the aquatic environment, we screened sewage and urban river water samples from miyazaki, japan for vre. because vancomycin-resistant organisms are not as prevalent in sewage and river water as vancomycin-susceptible organisms, the samples were screened by minimum inhibitory concentration test using the vancomycin-supplemented membrane-enterococcus indoxyl-β-d-glucoside (mei) agar. the isolat ... | 2015 | 25438128 |
| [enterococcal infections: from simple to most complex]. | enterococci are microorganisms with a remar- kable ability to adapt to their environment. two species have a significant clinical implication, enterococcus faecalis and enterococcus faecium. the risk factors for colonization and infection must be recognized, including prior treatment with antibiotics such as cephalosporins or quinolones. because of their native resistance to several classes of antibiotics and the increase of acquired resistance to penicillins, the initial empiric treatment of a ... | 2014 | 25438375 |
| enhanced free fatty acid production by codon-optimized lactococcus lactis acyl-acp thioesterase gene expression in escherichia coli using crude glycerol. | fatty acid production and composition are determined by the type of acyl-acyl carrier protein thioesterases (acyl-acp tes) expressed in escherichia coli. bacterial acyl-acp tes from lactococcus lactis (sgjs47), enterococcus faecalis (sgjs49), and burkholderia cepacia (sgjs50) were codon-optimized and expressed in e. coli for enhanced fatty acid production. samples were extracted at the lag, log, and stationary phases of cell growth, and gene expression levels of the codon optimized acy-acp tes a ... | 2014 | 25442943 |
| penicillin-resistant, ampicillin-susceptible enterococcus faecalis of hospital origin: pbp4 gene polymorphism and genetic diversity. | despite the spread of penicillin-resistant, ampicillin-susceptible enterococcus faecalis (prasef) isolates in diverse countries, the mechanisms leading to this unusual resistance phenotype have not yet been investigated. the aim of this study was to evaluate whether polymorphism in the pbp4 gene is associated with penicillin resistance in prasef isolates and to determine their genetic diversity. e. faecalis isolates were recovered from different clinical specimens of hospitalized patients from f ... | 2014 | 25445645 |
| antimicrobial and immunomodulatory properties of pgla-am1, cpf-am1, and magainin-am1: potent activity against oral pathogens. | cationic amphipathic α-helical peptides are intensively studied classes of host defence peptides (hdps). three peptides, peptide glycine-leucine-amide (pgla-am1), caerulein-precursor fragment (cpf-am1) and magainin-am1, originally isolated from norepinephrine-stimulated skin secretions of the african volcano frog xenopus amieti (pipidae), were studied for their antimicrobial and immunomodulatory activities against oral and respiratory pathogens. minimal effective concentrations (mecs), determine ... | 2014 | 25447193 |
| synthesis, antibacterial, antioxidant activity and qsar studies of novel 2-arylidenehydrazinyl-4-arylthiazole analogues. | a novel series of 2-arylidenehydrazinyl-4-arylthiazole analogues (3a-p) was designed and synthesized in excellent yields using a rapid, simple, efficient methodology. sixteen novel compounds were screened for in vitro antimicrobial activities against eleven bacteria, namely, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, klebsiella pneumonia, citrobacter freundii, cronobacter sakazakii, salmonella enteritidis, escherichia coli, yersinia pestis, and pseud ... | 2014 | 25450634 |
| new antimicrobial pregnane glycosides from the stem of ecdysanthera rosea. | phytochemical investigation on the stem of ecdysanthera rosea led to the isolation of eight new c-21 pregnane glycoside ecdysosides a-h (1-8), together with one known pregnane glycoside ecdysantheroside a (9). their structures were elucidated based on extensive spectroscopic data (ms, ir, 1d and 2d nmr) analysis, as well as comparison with the reported literature data. antimicrobial activities of all the compounds were evaluated against bacteria and yeasts. compounds 1, 9, 3 and 5 exhibited mode ... | 2014 | 25454459 |
| photoactivation of curcumin and sodium hypochlorite to enhance antibiofilm efficacy in root canal dentin. | to test the effect of ultrasonic or light activated curcumin and sodium hypochlorite against enterococcus faecalis biofilms in vitro. | 2015 | 25462576 |
| stable synthetic mono-substituted cationic bacteriochlorins mediate selective broad-spectrum photoinactivation of drug-resistant pathogens at nanomolar concentrations. | three stable synthetic mono-substituted cationic bacteriochlorins (bc37, bc38 and bc39) were recently reported to show exceptional activity (low nanomolar) in mediating photodynamic killing of human cancer cells after a 24h incubation upon excitation with near-infrared light (730 nm). the presence of cationic quaternary ammonium groups in each compound suggested likely activity as antimicrobial photosensitizers. herein this hypothesis was tested against a panel of pathogenic microorganisms that ... | 2014 | 25463659 |
| isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. | endosymbionts influence many aspects of their hosts' health conditions, including physiology, development, immunity, metabolism, etc. tree shrews (tupaia belangeri chinensis) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. to clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. based on morphologic ... | 2014 | 25465085 |
| antimicrobial activity of different filling pastes for deciduous tooth treatment. | guedes-pinto paste is the filling material most employed in brazil for endodontic treatment of deciduous teeth; however, the rifocort® ointment has been removed. thus, the aim of this study was to investigate the antimicrobial potential of filling pastes, by proposing three new pharmacological associations to replace rifocort® ointment with drugs of already established antimicrobial power: nebacetin® ointment, 2% chlorhexidine gluconate gel, and maxitrol® ointment. a paste composed of iodoform, ... | 2015 | 25466327 |
| antibacterial effect of calcium hydroxide combined with chlorhexidine on enterococcus faecalis: a systematic review and meta-analysis. | enterococcus faecalis (e. faecalis) is the most frequently isolated strain in failed endodontic therapy cases since it is resistant to calcium hydroxide (ch). whether a combination of ch and chlorhexidine (chx) is more effective than ch alone against e. faecalis is a matter of controversy. thus, the aim of this study was to conduct a systematic review and meta-analysis of the literature. | 2017 | 25466470 |
| an agmatine-inducible system for the expression of recombinant proteins in enterococcus faecalis. | scientific interest in enterococcus faecalis has increased greatly over recent decades. some strains are involved in food fermentation and offer health benefits, whereas others are vancomycin-resistant and cause infections that are difficult to treat. the limited availability of vectors able to express cloned genes efficiently in e. faecalis has hindered biotechnological studies on the bacterium's regulatory and pathogenicity-related genes. the agmatine deiminase (agdi) pathway of e. faecalis, i ... | 2014 | 25471381 |
| the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening. | the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ... | 2015 | 25475270 |
| atomic force microscopy visualization of injuries in enterococcus faecalis surface caused by er,cr:ysgg and diode lasers. | to visualize by atomic force microscopy the alterations induced on enterococcus. faecalis surface after treatment with 2 types of laser: erbium chromium:yttrium-scandium-gallium-garnet (er,cr:ysgg) laser and diode laser. | 2015 | 25475770 |
| in vitro activity of amazon plant extracts against enterococcus faecalis. | previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from brazilian forests' plants. in the present study, these extracts were subjected to microdilution broth assay (mdba) and disk diffusion assay (dda) using planktonic enterococcus faecalis atcc(®) 29212™ and were submitted to phytochemical analysis in tlc and hplc. three extracts obtained from ipomoea alba (mic < 40 μg/ml), diclinanona calycina (mic ≤ 40 μg/ml) and moronobea coccinea (40 ... | 2014 | 25477906 |
| the use of the replication region of plasmid prs7 from oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria. | a chimeric plasmid, prs7rep (6.1 kb), was constructed using the replication region of prs7, a large plasmid from oenococcus oeni, and pem64, a plasmid derived from pij2925 and containing a gene for resistance to chloramphenicol. prs7rep is a shuttle vector that replicates in escherichia coli using its pij2925 component and in lactic acid bacteria (lab) using the replication region of prs7. high levels of transformants per µg of dna were obtained by electroporation of prs7rep into pediococcus aci ... | 2015 | 25479060 |
| biosynthesis of silver nanoparticles using ethanolic petals extract of rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities. | the present study was aimed at biosynthesis of silver nanoparticles (agnps) using ethanolic extract of rose (rosa indica) petals and testing their potential antibacterial activity using selective human pathogenic microbes, anticancer activity using human colon adenocarcinoma cancer cell line hct 15 as well as anti-inflammatory activity using rat peritoneal macrophages in vitro. the biologically synthesized agnps were also characterized by uv-visible spectroscopy, scanning electron microscopy (se ... | 2015 | 25481491 |
| synergistic effect of 2% chlorhexidine combined with proteolytic enzymes on biofilm disruption and killing. | to investigate the dynamics of a disinfection regimen using 1% trypsin and 1% proteinase k in combination with 2% chlorhexidine (with or without ultrasonics) using a nutrient-stressed endodontic multispecies model biofilm. | 2015 | 25482847 |
| outcome of aortic valve replacement for active infective endocarditis in patients on chronic hemodialysis. | the high risk of morbidity and mortality for patients on hemodialysis who are undergoing cardiac surgery is increased for those with active infective endocarditis (aie). this retrospective observational single-center study evaluated the impact of chronic hemodialysis on the outcome of aortic valve replacement in patients with aortic aie. | 2015 | 25483000 |
| integration of non-oral bacteria into in vitro oral biofilms. | biofilms are polymicrobial communities that grow on surfaces in nature. oral bacteria can spontaneously form biofilms on the surface of teeth, which may compromise the health of the teeth, or their surrounding (periodontal) tissues. while the oral bacteria exhibit high tropism for their specialized ecological niche, it is not clear if bacteria that are not part of the normal oral microbiota can efficiently colonize and grow within oral biofilms. by using an in vitro "supragingival" biofilm model ... | 2015 | 25483866 |
| influence of trace elements mixture on bacterial diversity and fermentation characteristics of liquid diet fermented with probiotics under air-tight condition. | cu2+, zn2+, fe2+ and i- are often supplemented to the diet of suckling and early weaning piglets, but little information is available regarding the effects of different cu2+, zn2+, fe2+ and i- mixtures on bacteria growth, diversity and fermentation characteristics of fermented liquid diet for piglets. pyrosequencing was performed to investigate the effect of cu2+, zn2+, fe2+ and i- mixtures on the diversity, growth and fermentation characteristics of bacteria in the liquid diet fermented with ba ... | 2014 | 25486254 |
| transfer of tetracycline resistance genes with aggregation substance in food-borne enterococcus faecalis. | enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (as) and inducible sex pheromones to exchange genetic elements in food matrix. to evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne e. faecalis were collected in korea. among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(m) and tet(l) genes together, followed by tet(m) (19.6 %), and tet(l) (6.8 %) alone. also, we ... | 2015 | 25487115 |
| community-acquired methicillin-resistant staphylococcus epidermidis pyelonephritis in a child: a case report. | staphylococcus epidermidis is currently the most frequent pathogen of opportunistic and nosocomial infections worldwide. most cases of staphylococcus epidermidis infections are associated with indwelling medical devices and/or immunocompromised conditions. community-acquired urinary tract infections are rare, particularly among pediatric populations, and clinicians often do not consider staphylococcus epidermidis as a uropathogen. | 2014 | 25488491 |
| physicochemical and biological properties of oxovanadium(iv), cobalt(ii) and nickel(ii) complexes with oxydiacetate anions. | the potentiometric and conductometric titration methods have been used to characterize the stability of series of vo(iv)-, co(ii)- and ni(ii)-oxydiacetato complexes in dmso-water solutions containing 0-50 % (v/v) dmso. the influence of dmso as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (nbt) assay. the biological pro ... | 2015 | 25488702 |
| removal of total and antibiotic resistant bacteria in advanced wastewater treatment by ozonation in combination with different filtering techniques. | elimination of bacteria by ozonation in combination with charcoal or slow sand filtration for advanced sewage treatment to improve the quality of treated sewage and to reduce the potential risk for human health of receiving surface waters was investigated in pilot scale at the sewage treatment plant eriskirch, baden-wuerttemberg/germany. to determine the elimination of sewage bacteria, inflowing and leaving wastewater of different treatment processes was analysed in a culture-based approach for ... | 2015 | 25497174 |
| epidemiology and antibiotic susceptibility of aerococci in urinary cultures. | in this study, we present population-based data regarding the prevalence of aerococci in clinical urinary samples. during a 3-month period, all aerococcal isolates from urinary samples from 2 clinical microbiology laboratories were collected. we identified 64 aerococcus urinae isolates and 40 aerococcus sanguinicola isolates, which correlates with an incidence of 33 cases of aerococcal bacteriuria per 100,000 inhabitants per year. the median age was 83years for all patients with aerococcal bacte ... | 2015 | 25497460 |
| efficacy of antimicrobial photodynamic therapy for root canals infected with enterococcus faecalis. | effective decontamination of root canal systems is a constant concern in clinical practice. in this article, we compare the performance of photodynamic therapy (pdt) and sodium hypochlorite (naocl) in reducing the amount of enterococcus faecalis in root canals. relevant studies were identified by searching electronic databases, including web of science, pubmed, bvs (medline, scielo, lilacs and bbo), scopus, and cochrane, and by manually searching the references of identified studies. the terms u ... | 2014 | 25500925 |
| photodynamic therapy in root canals contaminated with enterococcus faecalis using curcumin as photosensitizer. | the aim of the study was to evaluate the photodynamic therapy (pdt) effect on root canals contaminated with enterococcus faecalis using a light emitting diode (led) light and a curcumin solution (cur) as photosensitizer (ps). eighty root canals from uniradicular human teeth were prepared with protaper universal rotary system and contaminated with e. faecalis for 21 days. they were divided as: gia-pdt (cur, pre-irradiation for 5 + 5 min of irradiation); gib-pdt (cur, pre-irradiation for 5 + 10 mi ... | 2015 | 25502831 |
| disinfection of gutta-percha cones using three reagents and their residual effects. | the purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and mtad in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. | 2014 | 25506147 |
| [enterococcal infections: from simple to most complex]. | enterococci are microorganisms with a remar- kable ability to adapt to their environment. two species have a significant clinical implication, enterococcus faecalis and enterococcus faecium. the risk factors for colonization and infection must be recognized, including prior treatment with antibiotics such as cephalosporins or quinolones. because of their native resistance to several classes of antibiotics and the increase of acquired resistance to penicillins, the initial empiric treatment of a ... | 2014 | 25507892 |
| [clinical efficacy of the antimicrobial drug furamag in nosocomial urinary tract infections]. | to evaluate the efficacy of the combined drug furamag (furasidine potassium and magnesium hydroxycarbonate) in combination with the third-generation cephalosporin cefotaxime versus cephalosporin monotherapy for nosocomial urinary tract infections (nuti). | 2014 | 25509895 |
| antimicrobial effect of ozonated water, sodium hypochlorite and chlorhexidine gluconate in primary molar root canals. | the aim was to determine the antimicrobial effect of ozonated water, ozonated water with ultrasonication, sodium hypochloride and chlorhexidine (chx) in human primary root canals contaminated by enterococcus faecalis (e. faecalis). | 2014 | 25512726 |
| antimicrobial activity of chlorhexidine, peracetic acid and sodium hypochlorite/etidronate irrigant solutions against enterococcus faecalis biofilms. | to evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (naocl) and associated with 9% hebp (naocl/hebp), 2% peracetic acid (paa) and 2% chlorhexidine (chx), on the viability of enterococcus faecalis biofilms attached to dentine. | 2015 | 25515403 |
| effect of a surfactant on the antimicrobial activity of sodium hypochlorite solutions. | the objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (naocl) associated with a surfactant. seventy single-rooted extracted human teeth were inoculated with enterococcus faecalis, and incubated for 21 days (37 °c). the groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% naocl; 5%, 2.5% and 1% hypoclean®, a solution containing a surfactant (cetrimide) associated with naocl. three microbiolo ... | 2016 | 25517777 |
| prevalence of enterococcus faecalis and porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: a molecular study. | because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. | 2014 | 25518530 |
| antimicrobial and antioxidant activities of alcoholic extracts obtained from vegetative organs of a. retroflexus. | in vitro antimicrobial and antioxidant activities of amaranthus retroflexus leaves and inflorescence alcoholic (ethanol 70%) extracts of various concentrations ranging from 0.78 to 400 μl/ml were analyzed on different clinical and reference bacterial strains (staphylococcus aureus, bacillus subtills, enterococcus faecalis, klebsiella pneumoniae, escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii) and fungal strains (candida albicans, c. famata, c. utilis, saccharomyces cerevisiae) ... | 2014 | 25518569 |
| effects of the probiotic enterococcus faecium ncimb 10415 on selected lactic acid bacteria and enterobacteria in co-culture. | enterococcus faecium ncimb 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. however, the mode of action of this probiotic modification is still unclear. one possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. reference strain ... | 2015 | 25519527 |
| evaluating the efficiency of humic acid to remove micro-organisms from denture base material. | to evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials. | 2016 | 25523597 |
| evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis. | photodynamic therapy (pdt) has been proven to be effective in disinfecting root canals. the aim of this present study was to evaluate the effects of pdt on the viability of enterococcus faecalis using methylene blue (mb) and malachite green (mg) as photosensitizers. solutions containing e. faecalis (atcc 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with mb and mg. samples were individually irradiated by the diode laser at a distance of 1mm for ... | 2014 | 25523956 |
| identification and antimicrobial resistance of enterococcus spp. isolated from the river and coastal waters in northern iran. | as fecal streptococci commonly inhabit the intestinal tract of humans and warm blooded animals, and daily detection of all pathogenic bacteria in coastal water is not practical, thus these bacteria are used to detect the fecal contamination of water. the present study examined the presence and the antibiotic resistance patterns of enterococcus spp. isolated from the babolrud river in babol and coastal waters in babolsar. seventy samples of water were collected in various regions of the babolrud ... | 2014 | 25525617 |
| effects of epigallocatechin gallate against enterococcus faecalis biofilm and virulence. | epigallocatechin-3-gallate (egcg), the most abundant polyphenol in green tea (camellia sinesis) has been shown to exert antimicrobial effects on numerous bacterial pathogens. however its efficacy against enterococcus faecalis biofilm, which is associated with persistent root canal infection is unknown. the aims of this study were to investigate the effects of egcg against e. faecalis biofilm and virulence. | 2015 | 25526623 |
| using a genome-scale metabolic model of enterococcus faecalis v583 to assess amino acid uptake and its impact on central metabolism. | increasing antibiotic resistance in pathogenic bacteria necessitates the development of new medication strategies. interfering with the metabolic network of the pathogen can provide novel drug targets but simultaneously requires a deeper and more detailed organism-specific understanding of the metabolism, which is often surprisingly sparse. in light of this, we reconstructed a genome-scale metabolic model of the pathogen enterococcus faecalis v583. the manually curated metabolic network comprise ... | 2015 | 25527553 |
| tyramine biosynthesis is transcriptionally induced at low ph and improves the fitness of enterococcus faecalis in acidic environments. | enterococcus faecalis is a commensal bacterium of the human gut that requires the ability to pass through the stomach and therefore cope with low ph. e. faecalis has also been identified as one of the major tyramine producers in fermented food products, where they also encounter acidic environments. in the present work, we have constructed a non-tyramine-producing mutant to study the role of the tyramine biosynthetic pathway, which converts tyrosine to tyramine via amino acid decarboxylation. wi ... | 2015 | 25529314 |
| syntheses, structures, and antimicrobial activity of new remarkably light-stable and water-soluble tris(pyrazolyl)methanesulfonate silver(i) derivatives of n-methyl-1,3,5-triaza-7-phosphaadamantane salt - [mpta]bf4. | two new silver(i) complexes of formula [ag(mpta)4](tpms)4(bf4) (1) and [ag(tpms)(mpta)](bf4) (2) (mpta = n-methyl-1,3,5-triaza-7-phosphaadamantane cation, tpms = tris(pyrazol-1-yl)methanesulfonate anion) have been synthesized and fully characterized by elemental analyses, (1)h and (31)p{(1)h} nmr, esi-ms, and ir spectroscopic techniques. the single-crystal x-ray diffraction study of 1 discloses a noncoordinated nature of the tpms species, existing as counterions around the highly charged metal c ... | 2015 | 25531979 |
| performance evaluation of a dna chip assay in the identification of major genitourinary pathogens. | to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections. | 2015 | 25533217 |
| transcriptome analysis of enterococcus faecalis during mammalian infection shows cells undergo adaptation and exist in a stringent response state. | as both a commensal and a major cause of healthcare-associated infections in humans, enterococcus faecalis is a remarkably adaptable organism. we investigated how e. faecalis adapts in a mammalian host as a pathogen by characterizing changes in the transcriptome during infection in a rabbit model of subdermal abscess formation using transcriptional microarrays. the microarray experiments detected 222 and 291 differentially regulated genes in e. faecalis og1rf at two and eight hours after subderm ... | 2014 | 25545155 |
| new role for human α-defensin 5 in the fight against hypervirulent clostridium difficile strains. | clostridium difficile infection (cdi), one of the most common hospital-acquired infections, is increasing in incidence and severity with the emergence and diffusion of hypervirulent strains. cdi is precipitated by antibiotic treatment that destroys the equilibrium of the gut microbiota. human α-defensin 5 (hd5), the most abundant enteric antimicrobial peptide, is a key regulator of gut microbiota homeostasis, yet it is still unknown if c. difficile, which successfully evades killing by other hos ... | 2015 | 25547793 |
| population biology of intestinal enterococcus isolates from hospitalized and nonhospitalized individuals in different age groups. | the diversity of enterococcal populations from fecal samples from hospitalized (n = 133) and nonhospitalized individuals (n = 173) of different age groups (group i, ages 0 to 19 years; group ii, ages 20 to 59 years; group iii, ages ≥60 years) was analyzed. enterococci were recovered at similar rates from hospitalized and nonhospitalized persons (77.44% to 79.77%) of all age groups (75.0% to 82.61%). enterococcus faecalis and enterococcus faecium were predominant, although seven other enterococcu ... | 2015 | 25548052 |