Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| composition and genetic diversity of mosquitoes (diptera: culicidae) on islands and mainland shores of kenya's lakes victoria and baringo. | the lake baringo and lake victoria regions of kenya are associated with high seroprevalence of mosquito-transmitted arboviruses. however, molecular identification of potential mosquito vector species, including morphologically identified ones, remains scarce. to estimate the diversity, abundance, and distribution of mosquito vectors on the mainland shores and adjacent inhabited islands in these regions, we collected and morphologically identified adult and immature mosquitoes and obtained the co ... | 2016 | 27402888 |
| pathogenesis of zika virus-associated embryopathy. | a strong causal association has become evident between zika virus (zikv) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the guillain-barré syndrome, are due to an endogenous form of hypervitaminosis a resulting from zikv infection-induced damage to the liver and the spillage of stored vitamin a compounds ("retinoids") into the ma ... | 2016 | 27403405 |
| efficacy of thai neem oil against aedes aegypti (l.) larvae. | trees with larvicidal activity may be found in thailand. we conducted this study to evaluate the efficacy and length of efficacy of thai neem (azadirachta siamensis) oil emulsion and an alginate bead of thai neem oil formulation against early fourth stage aedes aegypti larvae using a dipping test. the thai neem oil emulsion had significantly greater larvicidal activity than the alginate bead formulation at 12 to 60 hours post-exposure (p < 0.01). the thai neem oil formulation resulted in 100% mo ... | 2016 | 27405123 |
| transfer of dna from bacteria to eukaryotes. | historically, the members of the agrobacterium genus have been considered the only bacterial species naturally able to transfer and integrate dna into the genomes of their eukaryotic hosts. yet, increasing evidence suggests that this ability to genetically transform eukaryotic host cells might be more widespread in the bacterial world. indeed, analyses of accumulating genomic data reveal cases of horizontal gene transfer from bacteria to eukaryotes and suggest that it represents a significant fo ... | 2016 | 27406565 |
| a laboratory investigation into oviposition responses of aedes aegypti to some common household substances and water from conspecific larvae. | the surveillance of dengue vector - aedes aegypti, is routinely done by using ovitraps (a dark container filled with water and a wooden paddle/towel cloth provided as a oviposition substrate), which indicates the extent of aedes activity in an area. | 2011 | 27407765 |
| vector databank in the indian armed forces. | medical intelligence of disease vectors deals with understanding vector distribution and control. | 2011 | 27408077 |
| proceedings of the 3rd ipleiria's international health congress : leiria, portugal. 6-7 may 2016. | 2016 | 27409075 | |
| molecular diversity between salivary proteins from new world and old world sand flies with emphasis on bichromomyia olmeca, the sand fly vector of leishmania mexicana in mesoamerica. | sand fly saliva has been shown to have proteins with potent biological activities, salivary proteins that can be used as biomarkers of vector exposure, and salivary proteins that are candidate vaccines against different forms of leishmaniasis. sand fly salivary gland transcriptomic approach has contributed significantly to the identification and characterization of many of these salivary proteins from important leishmania vectors; however, sand fly vectors in some regions of the world are still ... | 2016 | 27409591 |
| larval food quantity affects the capacity of adult mosquitoes to transmit human malaria. | adult traits of holometabolous insects are shaped by conditions experienced during larval development, which might impact interactions between adult insect hosts and parasites. however, the ecology of larval insects that vector disease remains poorly understood. here, we used anopheles stephensi mosquitoes and the human malaria parasite plasmodium falciparum, to investigate whether larval conditions affect the capacity of adult mosquitoes to transmit malaria. we reared larvae in two groups; one ... | 0 | 27412284 |
| inhibitor designing, virtual screening, and docking studies for methyltransferase: a potential target against dengue virus. | aim of this work was to design and identify some s-adenosyl-l-homocysteine (sah) analogs as inhibitors of s-adenosyl-l-methionine-dependent methyltransferase (mtase) protein using computational approaches. | 0 | 27413346 |
| applications of a rapid and sensitive dengue duo rapid immunochromatographic test kit as a diagnostic strategy during a dengue type 2 epidemic in an urban city. | dengue infection is a major health problem in tropical and subtropical countries. a prospective observational study in a university-affiliated hospital was conducted between august 2015 and september 2015. patients who visited the emergency department (ed) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (ns1), igm/igg rapid immunochromatographic tests and real-time polymerase chain reaction (rt-pcr) to evaluate the performance of the rapid test ... | 2016 | 27415767 |
| larval application of sodium channel homologous dsrna restores pyrethroid insecticide susceptibility in a resistant adult mosquito population. | mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. the emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and zika. therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. herein we demonstrated the use of rna interference ... | 2016 | 27416771 |
| tyrosine hydroxylase is crucial for maintaining pupal tanning and immunity in anopheles sinensis. | tyrosine hydroxylase (th), the initial enzyme in the melanin pathway, catalyzes tyrosine conversion into dopa. although expression and regulation of th have been shown to affect cuticle pigmentation in insects, no direct functional studies to date have focused on the specific physiological processes involving the enzyme during mosquito development. in the current study, silencing of asth during the time period of continuous high expression in anopheles sinensis pupae led to significant impairmen ... | 2016 | 27416870 |
| receptors are affected by selection with each bacillus thuringiensis israelensis cry toxin but not with the full bti mixture in aedes aegypti. | bacillus thuringiensis israelensis (bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. by using rna sequencing, we investigated change in gene transcription level and polymorphism variations associated with resistance to each bti cry toxin and to the full bti toxin mixture in the dengue vector aedes aegypti. the up-regulation of genes related to chitin metabolism in a ... | 2016 | 27418233 |
| assessment of the ability of aedes species mosquitoes to transmit feline mycoplasma haemofelis and 'candidatus mycoplasma haemominutum'. | the objective of this study was to evaluate wild-caught mosquitoes for evidence of hemotropic mycoplasma species dna and to determine whether the feline hemoplasmas, mycoplasma haemofelis (mhf) and 'candidatus mycoplasma haemominutum' (mhm), can be transmitted by aedes aegypti mosquitoes in a laboratory setting. | 2016 | 27418288 |
| apoptosis-related genes control autophagy and influence denv-2 infection in the mosquito vector, aedes aegypti. | the mosquito aedes aegypti is the primary urban vector for dengue virus (denv) worldwide. insight into interactions occurring between host and pathogen is important in understanding what factors contribute to vector competence. however, many of the molecular mechanisms for vector competence remain unknown. our previous global transcriptional analysis suggested that differential expression of apoptotic proteins is involved in determining refractoriness vs susceptibility to denv-2 infection in ae. ... | 2016 | 27418459 |
| the effects of interspecific courtship on the mating success of aedes aegypti and aedes albopictus (diptera: culicidae) males. | satyrization, a form of asymmetric reproductive interference, has recently been shown to play a role in competitive displacements of aedes aegypti (l.) by aedes albopictus (skuse). furthermore, female ae. aegypti from populations in sympatry with ae. albopictus have evolved reproductive character displacement and changes in mating behavior to reduce interspecific mating. in this article, we examine evolutionary responses of males to interspecific mating and show that satyrization has also evoked ... | 2015 | 27418696 |
| resistance status to the insecticides temephos, deltamethrin, and diflubenzuron in brazilian aedes aegypti populations. | insecticides are still largely applied in public health to control disease vectors. in brazil, organophosphates (op) and pyrethroids (py) are used against aedes aegypti for years. since 2009 insect growth regulators (igr) are also employed in the control of larvae. we quantified resistance to temephos (op), deltamethrin (py), and diflubenzuron (igr) of a. aegypti samples from 12 municipalities distributed throughout the country, collected between 2010 and 2012. high levels of resistance to neuro ... | 2016 | 27419140 |
| time to empower release of insects carrying a dominant lethal and wolbachia against zika. | ridl (release of insects with dominant lethality) and wolbachia are 2 potentially powerful tools in the fight against zika, but their technological advancement is being hampered by policy barriers. in this study, we discuss what could be done to overcome these regulatory deadlocks. | 2016 | 27419175 |
| experiences with insecticide-treated curtains: a qualitative study in iquitos, peru. | dengue is an arthropod-borne viral disease responsible for approximately 400 million infections annually; the only available method of prevention is vector control. it has been previously demonstrated that insecticide treated curtains (itcs) can lower dengue vector infestations in and around houses. as part of a larger trial examining whether itcs could reduce dengue transmission in iquitos, peru, the objective of this study was to characterize the participants' experience with the itcs using qu ... | 2016 | 27422403 |
| two novel epistatic mutations (e1:k211e and e2:v264a) in structural proteins of chikungunya virus enhance fitness in aedes aegypti. | expansion of chikv outbreaks with appearance of novel mutations are reported from many parts of the world. two novel mutations viz. e1:k211e and e2:v264a in background of e1:226a are recently identified from aedes aegypti dominated areas of india. in this study, the role of these mutations in modulation of infectivity, dissemination and transmission by two different aedes species was studied. mutations were sequentially constructed in chikv genome and female ae. aegypti and aedes albopictus mosq ... | 2016 | 27423270 |
| a transgenic approach for controlling lygus in cotton. | lygus species of plant-feeding insects have emerged as economically important pests of cotton in the united states. these species are not controlled by commercial bacillus thuringiensis (bt) cotton varieties resulting in economic losses and increased application of insecticide. previously, a bt crystal protein (cry51aa2) was reported with insecticidal activity against lygus spp. however, transgenic cotton plants expressing this protein did not exhibit effective protection from lygus feeding dama ... | 2016 | 27426014 |
| effect of spatial repellent exposure on dengue vector attraction to oviposition sites. | aedes aegypti is a primary vector of dengue virus (denv), the causative agent of dengue fever, an arthropod-borne disease of global importance. although a vaccine has been recommended for prevention, current dengue prevention strategies rely on vector control. recently, volatile pyrethroids-spatial repellents-have received interest as a novel delivery system for adult ae. aegypti control. understanding the full range of behavioral effects spatial repellents elicit in mosquito species will be cri ... | 2016 | 27428011 |
| who is vulnerable to dengue fever? a community survey of the 2014 outbreak in guangzhou, china. | unprecedented dengue fever (df) outbreaks impel china to develop useful disease control strategies. integrated vector management (ivm) focuses on identifying vulnerable populations and interrupting human-vector contact; however, vulnerable populations have not been clearly identified in china. we conducted a case-control study during the initial stage of the 2014 df outbreak in guangzhou, china to assess risk factors for df infection. cases were randomly sampled from the national notifiable infe ... | 2016 | 27428986 |
| rna encapsidation and packaging in the phleboviruses. | the bunyaviridae represents the largest family of segmented rna viruses, which infect a staggering diversity of plants, animals, and insects. within the family bunyaviridae, the phlebovirus genus includes several important human and animal pathogens, including rift valley fever virus (rvfv), severe fever with thrombocytopenia syndrome virus (sftsv), uukuniemi virus (uukv), and the sandfly fever viruses. the phleboviruses have small tripartite rna genomes that encode a repertoire of 5-7 proteins. ... | 2016 | 27428993 |
| thymosin from bombyx mori is down-regulated in expression by bmnpv exhibiting antiviral activity. | thymosins have been highly conserved during evolution. these hormones exist in many animal species and play an essential role in many biological events. however, little is known regarding the physiological function of silkworm bombyx mori thymosin (bmthy). in this study, we investigated the expression pattern of bmthy in a bombyx mori larval ovarian cell line (bmn) challenged with bombyx mori nuclear polyhydrosis virus (bmnpv) and the antiviral effect of recombinant bmthy (rbmthy) for bombyx mor ... | 2016 | 27432352 |
| application of copper-based ovitraps in local houses in west sumatra, indonesia: a field test of a simple and affordable larvicide for mosquito control. | the application of oviposition traps (ovitraps) is one of the currently available rational methods used in mosquito control campaigns because it eliminates the larval stage. however, the use of current larvicides is hampered by their cost and applicability. therefore, a more economical and practical alternative is urgently needed. we previously reported that copper in liquid form is a promising candidate due to its potent larvicide properties in a laboratory setting, affordability, and availabil ... | 2016 | 27433130 |
| culex pipiens and aedes triseriatus mosquito susceptibility to zika virus. | 2016 | 27434194 | |
| vaccines and immunization strategies for dengue prevention. | dengue is currently the most significant arboviral disease afflicting tropical and sub-tropical countries worldwide. dengue vaccines, such as the multivalent attenuated, chimeric, dna and inactivated vaccines, have been developed to prevent dengue infection in humans, and they function predominantly by stimulating immune responses against the dengue virus (denv) envelope (e) and nonstructural-1 proteins (ns1). of these vaccines, a live attenuated chimeric tetravalent denv vaccine developed by sa ... | 2016 | 27436365 |
| outbreak of zika virus infection, chiapas state, mexico, 2015, and first confirmed transmission by aedes aegypti mosquitoes in the americas. | after decades of obscurity, zika virus (zikv) has spread through the americas since 2015 accompanied by congenital microcephaly and guillain-barré syndrome. although these epidemics presumably involve transmission by aedes aegypti, no direct evidence of vector involvement has been reported, prompting speculation that other mosquitoes such as culex quinquefasciatus could be involved. | 2016 | 27436433 |
| seasonal and geographical variation of dengue vectors in narathiwat, south thailand. | using gis-based land use map for the urban-rural division (the relative ratio of population density adjusted to relatively aedes-infested land area), we demonstrated significant independent observations of seasonal and geographical variation of aedes aegypti and aedes albopictus vectors between muang narathiwat district (urban setting) and neighbor districts (rural setting) of narathiwat, southern thailand, based on binomial distribution of aedes vectors in water-holding containers (water storag ... | 2016 | 27437001 |
| leukemoid reaction in chikungunya fever. | chikungunya is a viral illness caused by an arbovirus which is transmitted by aedes mosquito. fever and polyarthralgia are hallmark of this viral illness. viral infections are generally associated with leucopenia and bacterial infections with leukocytosis. leukemoid reaction (lr) is defined by reactive increase in leukocyte count of more than 50,000/cu mm with increase in mature leukocytes on peripheral blood. leukocytosis is common in chikungunya but leukemoid reaction has not been reported in ... | 2016 | 27437276 |
| historical environmental change in africa drives divergence and admixture of aedes aegypti mosquitoes: a precursor to successful worldwide colonization? | increasing globalization has promoted the spread of exotic species, including disease vectors. understanding the evolutionary processes involved in such colonizations is both of intrinsic biological interest and important to predict and mitigate future disease risks. the aedes aegypti mosquito is a major vector of dengue, chikungunya and zika, the worldwide spread of which has been facilitated by ae. aegypti's adaption to human-modified environments. understanding the evolutionary processes invo ... | 2016 | 27439067 |
| expression patterns, mutation detection and rna interference of rhopalosiphum padi voltage-gated sodium channel genes. | the voltage-gated sodium channel (vgsc) is the target of sodium-channel-blocking insecticides. traditionally, animals were thought to have only one vgsc gene comprising a α-subunit with four homologous domains (di-div). the present study showed that rhopalosiphum padi, an economically important crop pest, owned a unique heterodimeric vgsc (h1 and h2 subunits) encoded by two genes (rpvgsc1 and rpvgsc2), which is unusual in insects and other animals. the open reading frame (orf) of rpvgsc1 consist ... | 2016 | 27439594 |
| identification of a diazinon-metabolizing glutathione s-transferase in the silkworm, bombyx mori. | the glutathione s-transferase superfamily play key roles in the metabolism of numerous xenobiotics. we report herein the identification and characterization of a novel glutathione s-transferase in the silkworm, bombyx mori. the enzyme (bmgstu2) conjugates glutathione to 1-chloro-2,4-dinitrobenzene, as well as metabolizing diazinon, one of the organophosphate insecticides. quantitative reverse transcription-polymerase chain reaction analysis of transcripts demonstrated that bmgstu2 expression was ... | 2016 | 27440377 |
| gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development. | cold-induced mortality has historically been a key aspect of mountain pine beetle, dendroctonus ponderosae hopkins (coleoptera: curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. we used rna-seq analysis to monitor gene expression patterns of mountain pine beetle larvae at four time points during their overwintering period-early-autumn, late-autumn, early-spring, and late-spring. changing transcript profiles over the winter indica ... | 2016 | 27441109 |
| anti-dengue efficacy of bioactive andrographolide from andrographis paniculata (lamiales: acanthaceae) against the primary dengue vector aedes aegypti (diptera: culicidae). | the current study investigated the toxic effect of the leaf extract compound andrographolide from andrographis paniculata (burm.f) against the dengue vector ae. aegypti. gc-ms analysis revealed that andrographolide was recognized as the major chemical constituent with the prominent peak area compared with other compounds. all isolated toxic compounds were purified and confirmed through rp-hplc against chemical standards. the larvicidal assays established at 25ppm of bioactive compound against th ... | 2016 | 27443607 |
| physical influence on larvicidal and pupicidal activity of the silicone-based monomolecular film. | although silicone-based monomolecular film (mmf) has been accepted as larvicide in several countries, its mosquito control potential has never been investigated in thailand. laboratory assessment in this study was conducted to determine the mmf efficacy against aedes aegypti. at the recommended dosage (1ml/m(2) of water surface), mortality of pupae (99.17±0.83%) was significantly greater than mortality of old and young larvae (73.33±9.13, 11.67±3.47%; respectively). pupicidal activity was rapidl ... | 2016 | 27443608 |
| lmcyp4g102: an oenocyte-specific cytochrome p450 gene required for cuticular waterproofing in the migratory locust, locusta migratoria. | cytochrome p450 superfamily proteins play important roles in detoxification of xenobiotics and during physiological and developmental processes. to contribute to our understanding of this large gene family in insects, we have investigated the function of the cytochrome p450 gene lmcyp4g102 in the migratory locust locusta migratoria. suppression of lmcyp4g102 expression by rna interference (rnai) does not interfere with moulting but causes rapid loss of body weight - probably due to massive loss ... | 2016 | 27444410 |
| neuroethology of olfactory-guided behavior and its potential application in the control of harmful insects. | harmful insects include pests of crops and storage goods, and vectors of human and animal diseases. throughout their history, humans have been fighting them using diverse methods. the fairly recent development of synthetic chemical insecticides promised efficient crop and health protection at a relatively low cost. however, the negative effects of those insecticides on human health and the environment, as well as the development of insect resistance, have been fueling the search for alternative ... | 2016 | 27445858 |
| limited specificity in the injury and infection priming against bacteria in aedes aegypti mosquitoes. | injury and infection priming has been observed in several insect groups, reported as host immune protection against contact with a pathogen caused by a previous infection with the same. however, the specific response against a pathogen has not been demonstrated in all insect species. investigating the specific priming response in insects is important because their immune strategies probably reflect particular selective pressures exerted by different pathogens. here, we determined whether previou ... | 2016 | 27446016 |
| synthesis and insecticidal activity of novel pyrimidine derivatives containing urea pharmacophore against aedes aegypti. | aedes aegypti is a major mosquito vector for the transmission of serious diseases, especially dengue and yellow fever. more than 1 billion people in developing countries are at risk. the widespread and continual use of pesticides can lead to resistant mosquitoes. in order to maintain mosquito control gains, it is critical to develop and evaluate novel bioactive molecules that differ in mode of action from currently used products. | 2017 | 27448764 |
| a detailed comparative analysis of codon usage bias in zika virus. | zika virus (zikv) is a member of the family flaviviridae and its genome consists of a single-stranded positive sense rna molecule with 10,794 nucleotides. clinical manifestations of disease caused by zikv infection range from asymptomatic cases to an influenza-like syndrome. there is an increasing concern about the possible relation among microcephaly and zikv infection. to get insight into the relation of codon usage among viruses and their hosts is extremely important to understand virus survi ... | 2016 | 27449601 |
| synthesis and biological evaluation of 3,5-dimethoxystilbene analogs. | in our continuing effort to discover natural product-based pest management agents, derivatives of 3,5-dimethoxystilbene were synthesized yielding 27 new and six known compounds. compounds 11 and 12 showed strong aedes aegypti larvicidal activity (lc50 45.31 and 49.93 μm, respectively). furthermore, 11 and 12 exhibited high effectiveness against larvae of pesticide-susceptible and pyrethroid-resistant strains of ae. aegypti; activity against the adult mosquitoes was low. compounds 6f, 6g, and 6i ... | 2016 | 27450661 |
| the epidemiology and transmissibility of zika virus in girardot and san andres island, colombia, september 2015 to january 2016. | transmission of zika virus (zikv) was first detected in colombia in september 2015. as of april 2016, colombia had reported over 65,000 cases of zika virus disease (zvd). we analysed daily surveillance data of zvd cases reported to the health authorities of san andres and girardot, colombia, between september 2015 and january 2016. zvd was laboratory-confirmed by reverse transcription-polymerase chain reaction (rt-pcr) in the serum of acute cases within five days of symptom onset. we use daily i ... | 0 | 27452806 |
| determination and characterization of destruxin production in metarhizium anisopliae tk6 and formulations for aedes aegypti mosquitoes control at the field level. | destruxins, cyclic hexadepsipeptide toxins, secreted by the entomopathogenic fungus, metarhizium anisopliae through extracellular synthesis. the present study reports a new approach for the analysis of dtxs produced by the fungal strain metarhizium anisoliae tk6, using frir-hplc-lc-ms and h(1) nmr. the results also showed that production of the major dtxs a, b, c, and e have to be determined in czapek dextrose (cd) liquid culture filtrate from 9 to 12 days post-inoculation. purified dtx were fur ... | 2016 | 27452930 |
| correlation study between platelet count, leukocyte count, nonhemorrhagic complications, and duration of hospital stay in dengue fever with thrombocytopenia. | dengue is one of the common diseases presenting as fever with thrombocytopenia, also causing significant morbidity and complications. | 0 | 27453855 |
| selective factors associated with the evolution of codon usage in natural populations of arboviruses. | arboviruses (arthropod borne viruses) have life cycles that include both vertebrate and invertebrate hosts with substantial differences in vector and host specificity between different viruses. most arboviruses utilize rna for their genetic material and are completely dependent on host trnas for their translation, suggesting that virus codon usage could be a target for selection. in the current study we analyzed the relative synonymous codon usage (rscu) patterns of 26 arboviruses together with ... | 2016 | 27455096 |
| de novo assembly and characterization of the transcriptome of grasshopper shirakiacris shirakii. | the grasshopper shirakiacris shirakii is an important agricultural pest and feeds mainly on gramineous plants, thereby causing economic damage to a wide range of crops. however, genomic information on this species is extremely limited thus far, and transcriptome data relevant to insecticide resistance and pest control are also not available. | 2016 | 27455245 |
| infection rates by dengue virus in mosquitoes and the influence of temperature may be related to different endemicity patterns in three colombian cities. | colombia is an endemic country for dengue fever where the four serotypes of virus dengue (denv1-4) circulate simultaneously, and all types are responsible for dengue cases in the country. the control strategies are guided by entomological surveillance. however, heterogeneity in aedic indices is not well correlated with the incidence of the disease in cities such as riohacha, bello and villavicencio. as an alternative, molecular detection of dengue virus in mosquitoes has been proposed as a usefu ... | 2016 | 27455289 |
| viral rna silencing suppression: the enigma of bunyavirus nss proteins. | the bunyaviridae is a family of arboviruses including both plant- and vertebrate-infecting representatives. the tospovirus genus accommodates plant-infecting bunyaviruses, which not only replicate in their plant host, but also in their insect thrips vector during persistent propagative transmission. for this reason, they are generally assumed to encounter antiviral rna silencing in plants and insects. here we present an overview on how tospovirus nonstructural nss protein counteracts antiviral r ... | 2016 | 27455310 |
| purification and characterization of a novel kazal-type trypsin inhibitor from the leech of hirudinaria manillensis. | kazal-type serine proteinase inhibitors are found in a large number of living organisms and play crucial roles in various biological and physiological processes. although some kazal-type serine protease inhibitors have been identified in leeches, none has been reported from hirudinaria manillensis, which is a medically important leech. in this study, a novel kazal-type trypsin inhibitor was isolated from leech h. manillensis, purified and named as bdellin-hm based on the sequence similarity with ... | 2016 | 27455325 |
| the interplay of vaccination and vector control on small dengue networks. | dengue fever is a major public health issue affecting billions of people in over 100 countries across the globe. this challenge is growing as the invasive mosquito vectors, aedes aegypti and aedes albopictus, expand their distributions and increase their population sizes. hence there is an increasing need to devise effective control methods that can contain dengue outbreaks. here we construct an epidemiological model for virus transmission between vectors and hosts on a network of host populatio ... | 2016 | 27457093 |
| the insect-specific palm creek virus modulates west nile virus infection in and transmission by australian mosquitoes. | insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. these viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. following the isolation of the first australian insect-specific flavivirus (isf), palm creek virus (pcv), we investigated routes of infection and transmission of this virus in key australian arbovirus vectors and its impact on replication ... | 2016 | 27457250 |
| characterization of a midgut mucin-like glycoconjugate of lutzomyia longipalpis with a potential role in leishmania attachment. | leishmania parasites are transmitted by phlebotomine sand flies and a crucial step in their life-cycle is the binding to the sand fly midgut. laboratory studies on sand fly competence to leishmania parasites suggest that the sand flies fall into two groups: several species are termed "specific/restricted" vectors that support the development of one leishmania species only, while the others belong to so-called "permissive" vectors susceptible to a wide range of leishmania species. in a previous s ... | 2016 | 27457627 |
| opposing roles for dna replication initiator proteins orc1 and cdc6 in control of cyclin e gene transcription. | newly born cells either continue to proliferate or exit the cell division cycle. this decision involves delaying expression of cyclin e that promotes dna replication. orc1, the origin recognition complex (orc) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the preceding mitosis. we demonstrate that orc1 represses cyclin e gene (ccne1) transcription, an e2f1 activated gene that is also repressed by the retinoblastoma (rb) protein. orc1 binds to r ... | 2016 | 27458800 |
| ecotoxicological assessment of pyriproxyfen under environmentally realistic exposure conditions of integrated vector management for aedes aegypti control in brazil. | there is increasing concern to control aedes aegypti mosquito exposure in developing countries such as brazil. thus, integrated approaches using a combination of chemical, pyriproxyfen larvicide, and biological, xiphophorus maculatus, larvivorous fish species approaches are necessary and important to initiate more effective control against mosquito borne diseases. this study describes the toxicological effects of pyriproxyfen larvicide on the fish xiphophorus maculatus, the larvivorous fish spec ... | 2016 | 27458879 |
| a sequence of flushing and drying of breeding habitats of aedes aegypti (l.) prior to the low dengue season in singapore. | in dengue-endemic areas, transmission shows both a seasonal and interannual variability. to investigate how rainfall impacts dengue seasonality in singapore, we carried out a longitudinal survey in the geylang neighborhood from august 2014 to august 2015. the survey comprised of twice-weekly random inspections to outdoor breeding habitats and continuous monitoring for positive ones. in addition, observations of rainstorms were collected. out of 6824 inspected habitats, 67 contained aedes aegypti ... | 2016 | 27459322 |
| heat sensitivity of wmel wolbachia during aedes aegypti development. | the wmel strain of wolbachia bacteria is known to prevent dengue and zika virus transmission in the mosquito vector aedes aegypti. accordingly, the release of wmel-infected a. aegypti in endemic regions has been recommended by the world health organization as a potential strategy for controlling dengue and zika outbreaks. however, the utility of this approach could be limited if high temperatures in the aquatic habitats where a. aegypti develop are detrimental to wolbachia. we exposed wmel-infec ... | 2016 | 27459519 |
| additive effect of knockdown resistance mutations, s989p, v1016g and f1534c, in a heterozygous genotype conferring pyrethroid resistance in aedes aegypti in thailand. | mutation in the voltage-gated sodium channel gene that results in knockdown resistance (kdr), is a major mechanism of pyrethroid resistance in several mosquito species. in aedes aegypti, v1016g (occurring with and without s989p) and f1534c mutations are common and widely distributed throughout asia. the g1016 allele is known to be associated with resistance to type i and ii pyrethroids. the c1534 allele is primarily associated with resistance to type i pyrethroids and is known to be a recessive ... | 2016 | 27460671 |
| dynamic spatiotemporal trends of imported dengue fever in australia. | dengue fever (df) epidemics in australia are caused by infected international travellers and confined to northern queensland where competent vectors exist. recent analyses suggest that global trade and climate change could lead to the re-establishment of ae. aegypti across the country and promote the spread of dengue nationally. this study aimed to describe the dynamic spatiotemporal trends of imported df cases and their origins, identify the current and potential future high-risk regions and lo ... | 2016 | 27460696 |
| dengue deaths: associated factors and length of hospital stay. | background. dengue continues to pose a public health problem globally. objective. to review factors associated with patients who died from dengue in trinidad. methods. a retrospective case note review of hospitalized patients who died during 2001 to 2010. results. a total of 23 cases were identified: 13 males, 10 females-12 east indians, 9 africans, and 2 unknown. more than half (n = 17) were over 40 years of age with 10 being over 60 years of age; three were children. a falling platelet count w ... | 2016 | 27462472 |
| bioethical considerations at times of zika virus. | 0 | 27462882 | |
| comparative performance of transcriptome assembly methods for non-model organisms. | the technological revolution in next-generation sequencing has brought unprecedented opportunities to study any organism of interest at the genomic or transcriptomic level. transcriptome assembly is a crucial first step for studying the molecular basis of phenotypes of interest using rna-sequencing (rna-seq). however, the optimal strategy for assembling vast amounts of short rna-seq reads remains unresolved, especially for organisms without a sequenced genome. this study compared four transcript ... | 2016 | 27464550 |
| zika. an emerging teratogenic virus. | [no abstract available]. | 0 | 27464857 |
| storm drains as larval development and adult resting sites for aedes aegypti and aedes albopictus in salvador, brazil. | dengue (denv), chikungunya (chikv), zika (zikv), as well as yellow fever (yfv) viruses are transmitted to humans by aedes spp. females. in salvador, the largest urban center in north-eastern brazil, the four denv types have been circulating, and more recently, chikv and zikv have also become common. we studied the role of storm drains as aedes larval development and adult resting sites in four neighbourhoods of salvador, representing different socioeconomic, infrastructure and topographic condit ... | 2016 | 27464886 |
| preventing zika virus infection during pregnancy using a seasonal window of opportunity for conception. | it has come to light that zika virus (zikv) infection during pregnancy can result in trans-placental transmission to the fetus along with fetal death, congenital microcephaly, and/or central nervous system (cns) malformations. there are projected to be >9,200,000 births annually in countries with ongoing zikv transmission. in response to the zikv threat, the world health organization (who) is strategically targeting prevention of infection in pregnant women and funding contraception in epidemic ... | 2016 | 27467271 |
| zika virus: a new arboviral public health problem. | zika virus (zikv) is a single-stranded rna virus in the flaviviridae family and transmitted to human through infected mosquitos (aedes aegypti and aedes albopictus). virus is closely related with other flaviviruses; dengue virus, yellow fever virus, west nile virus, and japanese encephalitis virus phylogenetically. due to the possible relationship between virus and clinical features including microcephaly, ventricule, and eye deformities, guillain-barre syndrome increases the interest on this vi ... | 2016 | 27469112 |
| advances and perspectives in the application of crispr/cas9 in insects. | insects compose more than half of all living organisms on earth, playing essential roles in global ecosystems and forming complex relationships with humans. insect research has significant biological and practical importance. however, the application of genetic manipulation technology has long been restricted to several model insects only, such as gene knockout in drosophila, which has severely restrained the development of insect biology research. recently, with the increase in the release of i ... | 0 | 27469253 |
| chikugunya and zika virus dissemination in the americas: different arboviruses reflecting the same spreading routes and poor vector-control policies. | this review gathers the most recent investigations about chikungunya and zika viruses in america and would help in creating new research approaches. | 2016 | 27472289 |
| one-pot fabrication of silver nanocrystals using nicandra physalodes: a novel route for mosquito vector control with moderate toxicity on non-target water bugs. | mosquitoes (diptera: culicidae) as vectors for important diseases and parasites causing millions of deaths every year. the use of synthetic pesticides against culicidae leads to resistance and environmental concerns. therefore, eco-friendly control tools are a priority. in this research, nicandra physalodes-mediated synthesis of silver nanoparticles (ag nps) was conducted, in order to control larval populations of three important mosquito vectors, anopheles stephensi, aedes aegypti and culex qui ... | 2016 | 27473981 |
| dengue in a changing climate. | dengue is the world's most important arboviral disease in terms of number of people affected. over the past 50 years, incidence increased 30-fold: there were approximately 390 million infections in 2010. globalization, trade, travel, demographic trends, and warming temperatures are associated with the recent spread of the primary vectors aedes aegypti and aedes albopictus and of dengue. overall, models project that new geographic areas along the fringe of current geographic ranges for aedes will ... | 2016 | 27475051 |
| easy and inexpensive molecular detection of dengue, chikungunya and zika viruses in febrile patients. | dengue (denv), chikungunya (chikv) and zika (zikv) are arthropod-borne viruses (arboviruses) sharing a common vector, the mosquito aedes aegypti. at initial stages, patients infected with these viruses have similar clinical manifestations, however, the outcomes and clinical management of these diseases are different, for this reason early and accurate identification of the causative virus is necessary. this paper reports the development of a rapid and specific nested-pcr for detection of denv, c ... | 2016 | 27477452 |
| zika virus challenges for neuropsychiatry. | before 2007, zika virus (zikv) was generally considered as an arbovirus of low clinical relevance, causing a mild self-limiting febrile illness in tropical africa and southeast asia. currently, a large, ongoing outbreak of zikv that started in brazil in 2015 is spreading across the americas. virus infection during pregnancy has been potentially linked to congenital malformations, including microcephaly. in addition to congenital malformations, a temporal association between zikv infection and an ... | 2016 | 27478378 |
| potential for extrinsic incubation temperature to alter interplay between transmission potential and mortality of dengue-infected aedes aegypti. | the extrinsic incubation period is a critical component in the assessment of arboviral transmission potential. it defines the time it takes for a mosquito to become infectious following exposure to an arbovirus. since this is a temporal process, the lifespan of a mosquito is intimately tied to the extrinsic incubation period and thus transmission potential of these viruses. temperature is a known effector of both vector competence (the ability of a vector to transmit a pathogen) and mosquito mor ... | 2016 | 27478382 |
| artisanal extraction and traditional knowledge associated with medicinal use of crabwood oil (carapa guianensis aublet.) in a peri-urban várzea environment in the amazon estuary. | várzea forests of the amazon estuary contain species of importance to riverine communities. for example, the oil extracted from the seeds of crabwood trees is traditionally used to combat various illnesses and as such artisanal extraction processes have been maintained. the objectives of this study were to (1) describe the process involved in artisanal extraction of crabwood oil in the fazendinha protected area, in the state of amapá; (2) characterise the processes of knowledge transfer associat ... | 2016 | 27478479 |
| molecular characterization of an adiponectin receptor homolog in the white leg shrimp, litopenaeus vannamei. | adiponectin (adipoq) and its receptors (adipors) are strongly related to growth and development of skeletal muscle, as well as glucose and lipid metabolism in vertebrates. herein we report the identification of the first full-length cdna encoding an adipor homolog (liv-adipor) from the decapod crustacean litopenaeus vannamei using a combination of next generation sequencing (ngs) technology and bioinformatics analysis. the full-length liv-adipor (1,245 bp) encoded a protein that exhibited the ca ... | 2016 | 27478708 |
| using wolbachia releases to estimate aedes aegypti (diptera: culicidae) population size and survival. | mosquitoes carrying the endosymbiont bacterium wolbachia have been deployed in field trials as a biological control intervention due to wolbachia effects on reducing transmission of arboviruses. we performed mark, release and recapture (mrr) experiments using wolbachia as an internal marker with daily collections with bg-traps during the first two weeks of releases in rio de janeiro, brazil. the mrr design allowed us to investigate two critical parameters that determine whether wolbachia would s ... | 2016 | 27479050 |
| yellow fever-more a policy and planning problem than a biological one. | 2016 | 27479749 | |
| species-specific escape of plasmodium sporozoites from oocysts of avian, rodent, and human malarial parasites. | malaria is transmitted when an infected mosquito delivers plasmodium sporozoites into a vertebrate host. there are many species of plasmodium and, in general, the infection is host-specific. for example, plasmodium gallinaceum is an avian parasite, while plasmodium berghei infects mice. these two parasites have been extensively used as experimental models of malaria transmission. plasmodium falciparum and plasmodium vivax are the most important agents of human malaria, a life-threatening disease ... | 2016 | 27480269 |
| genome-wide identification and characterization of carboxypeptidase genes in silkworm (bombyx mori). | the silkworm (bombyx mori) is an economically-important insect that can secrete silk. carboxypeptidases have been found in various metazoan species and play important roles in physiological and biochemical reactions. here, we analyzed the silkworm genome database and characterized 48 carboxypeptidases, including 34 metal carboxypeptidases (bmmcp1-bmmcp34) and 14 serine carboxypeptidases (bmscp1-bmscp14), to better understand their diverse functions. compared to other insects, our results indicat ... | 2016 | 27483237 |
| the sex locus is tightly linked to factors conferring sex-specific lethal effects in the mosquito aedes aegypti. | in many taxa, sex chromosomes are heteromorphic and largely non-recombining. evolutionary models predict that spread of recombination suppression on the y chromosome is fueled by the accumulation of sexually antagonistic alleles in close linkage to the sex determination region. however, empirical evidence for the existence of sexually antagonistic alleles is scarce. in the mosquito aedes aegypti, the sex-determining chromosomes are homomorphic. the region of suppressed recombination, which surro ... | 2016 | 27485667 |
| tourniquet test for dengue diagnosis: systematic review and meta-analysis of diagnostic test accuracy. | dengue fever is a ubiquitous arboviral infection in tropical and sub-tropical regions, whose incidence has increased over recent decades. in the absence of a rapid point of care test, the clinical diagnosis of dengue is complex. the world health organisation has outlined diagnostic criteria for making the diagnosis of dengue infection, which includes the use of the tourniquet test (tt). | 2016 | 27486661 |
| zika virus: indian perspectives. | the emergence of zika virus (ziv), a mosquito borne flavivirus like dengue (den) and chikungunya (chik), in brazil in 2014 and its spread to various countries have led to a global health emergency. aedes aegypti is the major vector for ziv. fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ziv and there is abundance of aedes mosquitoes. in this review, we focus on current global scenar ... | 0 | 27487998 |
| racing against time against zika. | 0 | 27489331 | |
| microrna-309 targets the homeobox gene six4 and controls ovarian development in the mosquito aedes aegypti. | obligatory blood-triggered reproductive strategy is an evolutionary adaptation of mosquitoes for rapid egg development. it contributes to the vectorial capacity of these insects. therefore, understanding the molecular mechanisms underlying reproductive processes is of particular importance. here, we report that microrna-309 (mir-309) plays a critical role in mosquito reproduction. a spatiotemporal expression profile of mir-309 displayed its blood feeding-dependent onset and ovary-specific manife ... | 2016 | 27489347 |
| prospects for dengue vaccines for travelers. | travel-acquired dengue cases have been increasing as the overall global dengue burden has expanded. in korea, imported dengue cases have been reported since 2000 when it first became a notifiable disease. during the first four months of 2016, three times more dengue cases were reported in korea than during the same period the previous year. a safe and efficacious vaccine for travelers would be beneficial to prevent dengue disease in individual travelers and potentially decrease the risk of virus ... | 2016 | 27489798 |
| update: ongoing zika virus transmission - puerto rico, november 1, 2015-july 7, 2016. | zika virus is a flavivirus transmitted primarily by aedes aegypti and aedes albopictus mosquitoes, and infection can be asymptomatic or result in an acute febrile illness with rash (1). zika virus infection during pregnancy is a cause of microcephaly and other severe birth defects (2). infection has also been associated with guillain-barré syndrome (gbs) (3) and severe thrombocytopenia (4,5). in december 2015, the puerto rico department of health (prdh) reported the first locally acquired case o ... | 2016 | 27490087 |
| contraceptive use among nonpregnant and postpartum women at risk for unintended pregnancy, and female high school students, in the context of zika preparedness - united states, 2011-2013 and 2015. | zika virus infection during pregnancy can cause congenital microcephaly and brain abnormalities (1,2). since 2015, zika virus has been spreading through much of the world health organization's region of the americas, including u.s. territories. zika virus is spread through the bite of aedes aegypti or aedes albopictus mosquitoes, by sex with an infected partner, or from a pregnant woman to her fetus during pregnancy.* cdc estimates that 41 states are in the potential range of aedes aegypti or ae ... | 2016 | 27490117 |
| one-pot biogenic fabrication of silver nanocrystals using quisqualis indica: effectiveness on malaria and zika virus mosquito vectors, and impact on non-target aquatic organisms. | currently, mosquito vector control is facing a number of key challenges, including the rapid development of resistance to synthetic pesticides and the recent spread of aggressive arbovirus outbreaks. the biosynthesis of silver nanoparticles (agnps) is currently considered an environmental friendly alternative to the employ of pyrethroids, carbamates and microbial agents (e.g. bacillus thuringiensis var. israelensis), since agnps are easy to produce, effective and stable in the aquatic environmen ... | 2016 | 27491031 |
| knockdown resistance mutations in aedes aegypti (diptera: culicidae) from puerto rico. | permethrin resistance is widespread in aedes aegypti (l.), the main dengue, zika, and chikungunya virus vector in latin america and the caribbean. a common mechanism of resistance to pyrethroids-knockdown resistance (kdr)-is conferred through mutations in the insect's voltage-dependent sodium channel. in this mosquito, around 10 replacement substitutions in the voltage-gated sodium channel gene (vgsc) have been reported in pyrethroid-resistant strains. two of these mutations, named ile1,016 and ... | 2016 | 27493252 |
| coexistence of aedes aegypti and aedes albopictus (diptera: culicidae) in peninsular florida two decades after competitive displacements. | the spread of aedes albopictus (skuse) eastward in the mid-1980s from its initial establishment in houston, tx, was associated with rapid declines and local disappearances of aedes aegypti (l.) in gulf coast states and florida where annual larval surveillance during the early 1990s described temporal and spatial patterns of competitive displacements in cemeteries and tire shops. approximately 20 yr later in 2013-2014, we re-visited former collection sites and sampled aquatic immatures of these t ... | 2016 | 27493253 |
| persistence of neutralizing antibody against dengue virus 2 after 70 years from infection in nagasaki. | this study aimed to investigate the duration of humoral immune responses to dengue virus (denv) infection in japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in nagasaki, japan, from 1942 to 1944. a japanese volunteer requested serological diagnosis of denv infection in 2014 and donated blood sample to measure the antibody titer against denv by antiflavi igg indirect elisa, focus reduction neutralization test, and pl ... | 2016 | 27493841 |
| rise and fall of vector infectivity during sequential strain displacements by mosquito-borne dengue virus. | each of the four serotypes of mosquito-borne dengue virus (denv-1-4) comprises multiple, genetically distinct strains. competitive displacement between strains within a serotype is a common feature of denv epidemiology and can trigger outbreaks of dengue disease. we investigated the mechanisms underlying two sequential displacements by denv-3 strains in sri lanka that each coincided with abrupt increases in dengue haemorrhagic fever (dhf) incidence. first, the post-dhf strain displaced the pre-d ... | 2016 | 27500505 |
| calculation of the average cost per case of dengue fever in mexico using a micro-costing approach. | the increasing burden of dengue fever (df) in the americas, and the current epidemic in previously unaffected countries, generate major costs for national healthcare systems. there is a need to quantify the average cost per df case. in mexico, few data are available on costs, despite df being endemic in some areas. extrapolations from studies in other countries may prove unreliable and are complicated by the two main mexican healthcare systems (the secretariat of health [ss] and the mexican soci ... | 2016 | 27501146 |
| the critical role of early dengue surveillance and limitations of clinical reporting - implications for non-endemic countries. | the increasing dengue burden and epidemic severity worldwide have highlighted the need to improve surveillance. in non-endemic areas such as taiwan, where outbreaks start mostly with imported cases from southeast asia, a closer examination of surveillance dynamics to detect cases early is necessary. to evaluate problems with dengue surveillance and investigate the involvement of different factors at various epidemic stages, we investigated 632 laboratory-confirmed indigenous dengue cases in kaoh ... | 2016 | 27501302 |
| larvicidal activity of natural and modified triterpenoids against aedes aegypti (diptera: culicidae). | insecticide resistance to commonly used substances demands new molecules for the chemical control of the dengue vector aedes aegypti. because natural product sources have been an alternative to obtain larvicidal compounds, the aim of this study was to evaluate the triterpenoids betulinic (ba) and ursolic (ua) acids and their semi-synthetic derivatives against larval ae. aegypti. ba, ua, ten derivatives modified at the c-3 position and a positive control (diflubenzuron) were evaluated. larvicidal ... | 2016 | 27501778 |
| identification of aacasps7, an effector caspase in aedes albopictus. | aedes albopictus mosquito is a vector of various arboviruses and is becoming a significant threat to public health due to its rapid global expansion. several reports suggest that apoptosis could be a factor limiting arbovirus infection in mosquitoes. thus, it is significant to identify apoptosis pathway and study the correlation between apoptosis and virus infection in mosquitoes. apoptosis is a type of programmed cell death that plays a vital role in immunity, development, and tissue homeostasi ... | 2016 | 27502418 |
| systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs. | acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ... | 2016 | 27502490 |
| computational characterization of iron metabolism in the tsetse disease vector, glossina morsitans: ire stem-loops. | iron metabolism and regulation is an indispensable part of species survival, most importantly for blood feeding insects. iron regulatory proteins are central regulators of iron homeostasis, whose binding to iron response element (ire) stem-loop structures within the utrs of genes regulate expression at the post-transcriptional level. despite the extensive literature on the mechanism of iron regulation in human, less attention has been given to insect and more specifically the blood feeding insec ... | 2016 | 27503259 |