Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| structures of new phenolics isolated from licorice, and the effectiveness of licorice phenolics on vancomycin-resistant enterococci. | licorice, which is the underground part of glycyrrhiza species, has been used widely in asian and western countries as a traditional medicine and as a food additive. our continuous investigation on the constituents of roots and stolons of glycyrrhiza uralensis led to the isolation of two new phenolics, in addition to 14 known compounds. structural studies including spectroscopic and simple chemical derivatizations revealed that both of the new compounds had 2-aryl-3-methylbenzofuran structures. ... | 2014 | 25157467 |
| evaluation of enterococcus faecalis clinical isolates with 'penicillin-resistant, ampicillin-susceptible' phenotype as reported by vitek-2 compact system. | it has been recently reported that ampicillin susceptibility cannot accurately predict piperacillin and imipenem susceptibilities in penicillin-resistant, ampicillin-susceptible (pen-r, amp-s) enterococcus faecalis isolates, contrary to the current clinical and laboratory standards institute (clsi) recommendations. this has important therapeutic implications. such isolates were noted after the use of vitek-2 compact system ast-gp67 susceptibility cards in a singapore general hospital and they we ... | 2014 | 25158809 |
| which tree orders in southern africa have the highest antimicrobial activity and selectivity against bacterial and fungal pathogens of animals? | the study randomly screened leaf extracts of several hundred southern african tree species against important microbial pathogens to determine which taxa have the highest activity and may yield useful products to treat infections in the animal health market. | 2014 | 25164197 |
| what's new in the treatment of enterococcal endocarditis? | enterococcus spp. are among the common pathogens causing infective endocarditis (ie). despite major medical advances and new potent antimicrobial agents, the mortality has not significantly improved for several decades. the usual lack of bactericidal activity of penicillin or ampicillin, the toxicity from the combination of penicillin plus aminoglycosides, and the increased reports of high-level resistance to aminoglycosides have led to the exploration of other regimens for treatment of enteroco ... | 2014 | 25165018 |
| the influence of urinary ph on antibiotic efficacy against bacterial uropathogens. | to determine the effects of ph on the activity of clinically relevant antibiotics against bacterial uropathogens. numerous factors affect antibiotic efficacy within the urinary tract including ph. because human urine can substantially vary from acidic (ph 4.5) to alkaline (ph 8) conditions and can be easily clinically manipulated, it would be a great advantage to better understand the role of ph in antibiotic treatment of urinary tract infection. | 2014 | 25168568 |
| vapor-induced transfer of bacteria in the absence of mechanical disturbances. | transfer of bacteria through water vapor generated at moderate temperatures (30-50°c) in passive solar stills, has scarcely been reported. the objective of this research was to investigate whether bacteria in highly humid atmospheres can get transferred through water vapor in the absence of other transfer media to find their way to the distillate. to achieve this objective, passive solar reactors were chosen as the medium for experimentation, and distillation experiments were conducted by spikin ... | 2014 | 25169809 |
| enterococcus faecalis infection and reactive oxygen species down-regulates the mir-17-92 cluster in gastric adenocarcinoma cell culture. | chronic inflammation due to bacterial overgrowth of the stomach predisposes to the development of gastric cancer and is also associated with high levels of reactive oxygen species (ros). in recent years increasing attention has been drawn to micrornas (mirnas) due to their role in the pathogenesis of many human diseases including gastric cancer. here we studied the impact of infection by the gram-positive bacteria enterococcus faecalis (e. faecalis) on global mirna expression as well as the effe ... | 2014 | 25170597 |
| control of tyramine and histamine accumulation by lactic acid bacteria using bacteriocin forming lactococci. | the aim of this study was to evaluate the competitive effects of three bacteriocin producing strains of lactococcus lactis subsp. lactis against two aminobiogenic lactic acid bacteria, i.e. the tyramine producing strain enterococcus faecalis ef37 and the histamine producing strain streptococcus thermophilus pri60, inoculated at different initial concentrations (from 2 to 6 log cfu/ml). the results showed that the three l. lactis subsp. lactis strains were able to produce bacteriocins: in particu ... | 2014 | 25173915 |
| evaluation of detachment methods for the enumeration of bacteroides fragilis in sediments via propidium monoazide quantitative pcr, in comparison with enterococcus faecalis and escherichia coli. | the aim was to develop an optimized detachment method for separating bacteroidales from sediments to allow enumeration via pma-qpcr. the effectiveness of four different detachment treatments in removing bacteroides fragilis was compared as a function of time as well as in relation to enterococcus faecalis and escherichia coli as detected by cultivation and qpcr. | 2014 | 25175698 |
| antibacterial validation of electrogenerated hypochlorite using carbon-based electrodes. | this proof-of-concept study explores the novel use of carbon-based electrodes for the electrochemical generation of hypochlorite and compares the antimicrobial efficacy against commercial hypochlorite solution. antimicrobial concentrations of hypochlorite were generated using pad-printed carbon and carbon fibre electrodes, yielding up to 0·027% hypochlorite in 60 min and 0·1% hypochlorite in 15 min, respectively, in a nondivided assembly. the minimum inhibitory concentration (mic) of the electro ... | 2014 | 25179583 |
| antimicrobial activity of some essential oils against oral multidrug-resistant enterococcus faecalis in both planktonic and biofilm state. | to evaluate some essential oils in treatment of intractable oral infections, principally caused by biofilm of multidrug-resistant enterococcus faecalis (e. faecalis), such as persistent endodontic infections in which their treatment exhibits a real challenge for dentists. | 2014 | 25182948 |
| using mineral trioxide aggregate powder as an apical barrier: a bacterial leakage study. | mineral trioxide aggregate (mta) has been used successfully for the treatment of necrotic immature teeth as part of a 2-step apical barrier. recently, some researchers have used mta powder instead of wet-mix mta. it has also been used in a single-step approach in order to decrease the number of patient appointments. the aim of this study was to evaluate the bacterial microleakage of mta powder in comparison with wet-mix mta, in single or 2-step barrier approaches. this ex vivo study was carried ... | 2014 | 25184718 |
| surface free energy activated high-throughput cell sorting. | cell sorting is an important screening process in microbiology, biotechnology, and clinical research. existing methods are mainly based on single-cell analysis as in flow cytometric and microfluidic cell sorters. here we report a label-free bulk method for sorting cells by differentiating their characteristic surface free energies (sfes). we demonstrated the feasibility of this method by sorting model binary cell mixtures of various bacterial species, including pseudomonas putida kt2440, enteroc ... | 2014 | 25184988 |
| impact of minimal inhibitory concentration breakpoints on local cumulative bacterial susceptibility data and antibiotic consumption. | the phenotypic antimicrobial susceptibility testing (ast) of bacteria depends on minimal inhibitory concentration breakpoints issued by national and international breakpoint committees. the current study was performed in order to test the influence of different ast standards on local cumulative ast data and on antibiotic consumption. | 2014 | 25185565 |
| time-dependent antibacterial effects of aloe vera and zataria multiflora plant essential oils compared to calcium hydroxide in teeth infected with enterococcus faecalis. | in the present in vitro study, we investigated the time-related antimicrobial efficacy of aloe vera and zataria multiflora (z. multiflora) plant essential oils compared to calcium hydroxide ([ca[oh]2 ) to eliminate enterococcus faecalis (e. faecalis) from root canals. | 2016 | 25187255 |
| development of an antibacterial root canal filling system containing mdpb. | an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (mdpb)-containing experimental, chemically cured primer was prepared to develop a new resin-based root canal filling system. this study investigated the antibacterial effects of the mdpb-containing primer (experimental primer [ep]) against enterococcus faecalis and assessed the in vitro bonding and sealing abilities of the filling system, consisting of ep and a bis-gma-based sealer resin. antibacterial effects of ep were evaluat ... | 2014 | 25192898 |
| daptomycin in the treatment of prosthetic joint infection by enterococcus faecalis: safety and efficacy of high-dose and prolonged therapy. | enterococci are implicated in less than 2.3% of prosthetic joint infections. these infections can be difficult to treat and therapeutic failures are not uncommon. in these situations, daptomycin is a safe and effective alternative. we present a clinical case with a successful response to the prolonged use of high-dose daptomycin. | 2014 | 25192907 |
| the effect of sodium hypochlorite on enterococcus faecalis when grown on dentine as a single- and multi-species biofilm. | enterococcus faecalis is often involved in the aetiology of apical periodontitis after endodontic treatment. this project aimed to establish, on dentine in vitro, a multi-species biofilm containing e. faecalis, and to determine if the organism had an increased resistance to sodium hypochlorite compared with an axenic biofilm. biofilms were established on dentine discs in flow cells with either e. faecalis alone (axenic) or together with fusobacterium nucleatum and streptococcus sanguinis. follow ... | 2014 | 25195495 |
| structure of the double-stranded dna-binding type iv secretion protein tran from enterococcus. | conjugative transfer through type iv secretion multiprotein complexes is the most important means of spreading antimicrobial resistance. plasmid pip501, frequently found in clinical enterococcus faecalis and enterococcus faecium isolates, is the first gram-positive (g+) conjugative plasmid for which self-transfer to gram-negative (g-) bacteria has been demonstrated. the pip501-encoded type iv secretion system (t4ss) protein tran localizes to the cytoplasm and shows specific dna binding. the spec ... | 2014 | 25195751 |
| bimix antimicrobial scaffolds for regenerative endodontics. | eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. the aim of this study was to synthesize and determine in vitro both the antimicrobial effectiveness and cytocompatibility of bimix antibiotic-containing polydioxanone-based polymer scaffolds. | 2014 | 25201643 |
| changing pattern of infectious agents in postneurosurgical meningitis. | the rates of postneurosurgical infections, particularly meningitis and the most common pathogens have been reported variable during the recent years. the aim of this research was to determine the prevalence of postneurosurgical meningitis and its current spectrum of infectious agent. | 2014 | 25202446 |
| enzymatic elucidation of haemocyanin from kuruma shrimp marsupenaeus japonicus and its molecular recognition mechanism towards pathogens. | haemocyanin is an important non-specific immune protein present in the hemolymph of invertebrates, which have the ability to recognize the microbial pathogens and trigger the innate immune system. in this study, we isolated and purified the haemocyanin using gel filtration chromatography and investigated its microbial recognition mechanism against the invading pathogens. kuruma shrimp marsupenaeus japonicus haemocyanin showed the single band with a molecular weight of 76 kda on sds-page and its ... | 2015 | 25204648 |
| antimicrobial efficacy of endodontic irrigants against enterococcus faecalis and escherichia coli: an in vitro study. | to evaluate the relative antimicrobial efficacy of five different commonly used antimicrobial agents with regard to reduction in the number of enterococcus faecalis and escherichia coli as compared to normal saline. an agar disk diffusion in vitro method was used to test the efficacy of the root canal irrigants against these two microorganisms. the root canal irrigants used were: 5.25% sodium hypochlorite (naocl), 10% citric acid, 17% ethylene diamine tetraacetic acid (edta), 3% hydrogen peroxid ... | 2013 | 25206218 |
| driving forces of vancomycin-resistant e. faecium and e. faecalis blood-stream infections in children. | rates of invasive vancomycin-resistant enterococcus (vre) in the usa remains on the rise. efforts to control vancomycin use and nosocomial transmission have had limited success in halting the spread of this pathogen. the role of antibiotic exposure remains a topic of controversy. we evaluated the association between emergence of vre-blood-stream infections (bsi), aggregate and individual-patient vancomycin- exposure, and clonal transmission of vre at an academic pediatric tertiary care hospital. | 2014 | 25206975 |
| presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men. | urogenital tract infections in males are one of the significant etiological factors in infertility. in this prospective study, 72 patients with abnormal semen parameters or any other symptoms of urogenital tract infection were examined. semen analysis according to the who 2010 manual was performed together with microbial assessment: aerobic bacteria culture, chlamydia antigen test, candida culture, ureaplasma and mycoplasma-specific culture. in total, 69.4% of semen samples were positive for at ... | 2015 | 25209133 |
| healthcare-associated infective endocarditis: a case series in a referral hospital from 2006 to 2011. | background: healthcare-associated infective endocarditis (hca-ie), a severe complication of medical care, shows a growing incidence in literature. objective: to evaluate epidemiology, etiology, risk factors for acquisition, complications, surgical treatment, and outcome of hca-ie. methods: observational prospective case series study (2006-2011) in a public hospital in rio de janeiro. results: fifty-three patients with hca-ie from a total of 151 cases of infective endocarditis (ie) were included. ... | 2014 | 25211203 |
| draft genome sequence of an enterococcus faecalis strain isolated from a neonatal blood sepsis patient. | herein, we report the draft genome sequence of enterococcus faecalis ed-ngs-1009, cultivated from a blood sample taken from a neonatal sepsis patient at the royal infirmary in edinburgh, scotland, united kingdom. | 2014 | 25212626 |
| synergistic and antagonistic effect of lactic acid bacteria on tyramine production by food-borne pathogenic bacteria in tyrosine decarboxylase broth. | the effect of lactic acid bacteria (lab) strains on tyramine (tyr) and also other biogenic amines (ba) production by eight common food-borne pathogen (fbp) in tyrosine decarboxylase broth (tdb) was investigated by using a rapid hplc method. significant differences were observed among the fbp strains in ammonia (amn) and ba production apart from tryptamine, histamine (his) and spermine formation (p<0.05). salmonella paratyphi a was characterised as the main amine producer. lab had an important sy ... | 2011 | 25214109 |
| the cyclic dinucleotide c-di-amp is an allosteric regulator of metabolic enzyme function. | cyclic di-adenosine monophosphate (c-di-amp) is a broadly conserved second messenger required for bacterial growth and infection. however, the molecular mechanisms of c-di-amp signaling are still poorly understood. using a chemical proteomics screen for c-di-amp-interacting proteins in the pathogen listeria monocytogenes, we identified several broadly conserved protein receptors, including the central metabolic enzyme pyruvate carboxylase (lmpc). biochemical and crystallographic studies of the l ... | 2014 | 25215494 |
| construction of a chimeric lysin ply187n-v12c with extended lytic activity against staphylococci and streptococci. | developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. in the present work, a novel chimeric lysin (ply187n-v12c) was constructed by fusing the catalytic domain (ply187n) of the bacteriophage lysin ply187 with the cell binding domain (146-314aa, v12c) of the lysin plyv12. the results showed that the chimeric lysin ply187n-v12c had not only lytic activity similar to ply187n against staphylococcal strains but al ... | 2015 | 25219798 |
| control of listeria monocytogenes in fresh cheese using protective lactic acid bacteria. | in the past years, there has been a particular focus on the application of bacteriocins produced by lactic acid bacteria (lab) in controlling the growth of pathogenic bacteria in foods. the aim of this study was to select lab strains with antimicrobial activity, previously isolated from a traditional azorean artisanal cheese (pico cheese), in order to identify those with the greatest potential in reducing listeria monocytogenes in fresh cheese. eight bacteriocin producer strains identified as la ... | 2014 | 25222327 |
| antibacterial properties of root canal lubricants: a comparison with commonly used irrigants. | the aim was to assess in vitro the antibacterial activity of 10 root canal lubricants. k-y jelly personal lubricant, rc-prep, file-eze, file-rite, endoprep gel, endosure prep crème 15%, prep-rite, glyde, slickgel es and alpha glide were selected and compared in their antimicrobial properties to seven irrigants. serial dilutions of each agent in tryptic soy broth were inoculated with either enterococcus faecalis or pseudomonas aeruginosa and incubated at 37c for 24 h. during incubation bacterial ... | 2014 | 25223525 |
| carbamidocyclophanes f and g with anti-mycobacterium tuberculosis activity from the cultured freshwater cyanobacterium nostoc sp. | two new (1 and 2) and three known (3-5) carbamidocyclophanes were isolated from a cultured freshwater cyanobacterium nostoc sp. (uic 10274) obtained from a sample collected at des plaines, illinois. their planar structures and stereoconfigurations were determined by extensive spectroscopic analysis including 1d/2d nmr experiments, hresims as well as cd spectroscopy. carbamidocyclophane f (1) showed potent anti-mycobacterium tuberculosis activity in the microplate alamar blue assay and low-oxygen ... | 2014 | 25225453 |
| antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials propolis and aloe vera against enterococcus faecalis: an ex vivo study. | the aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, propolis and aloe vera on enterococcus faecalis in deep dentin. | 2014 | 25225560 |
| highly diverse microbiota in dental root canals in cases of apical periodontitis (data of illumina sequencing). | chronic apical periodontitis (cap) is a frequent condition that has a considerable effect on a patient's quality of life. we aimed to reveal root canal microbial communities in antibiotic-naive patients by applying illumina sequencing (illumina inc, san diego, ca). | 2014 | 25227214 |
| ebpa vaccine antibodies block binding of enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice. | enterococci bacteria are a frequent cause of catheter-associated urinary tract infections, the most common type of hospital-acquired infection. treatment has become increasingly challenging because of the emergence of multiantibiotic-resistant enterococcal strains and their ability to form biofilms on catheters. we identified and targeted a critical step in biofilm formation and developed a vaccine that prevents catheter-associated urinary tract infections in mice. in the murine model, formation ... | 2014 | 25232179 |
| plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: an in vitro study. | autologous platelet concentrates are successfully adopted in a variety of medical fields to stimulate bone and soft tissue regeneration. the rationale for their use consists in the delivery of a wide range of platelet-derived bioactive molecules that promotes wound healing. in addition, antimicrobial properties of platelet concentrates have been pointed out. in this study, the effect of the platelet concentration, of the activation step and of the presence of plasmatic components on the antimicr ... | 2014 | 25232963 |
| recurrent linezolid-resistant enterococcus faecalis infection in a patient with pneumonia. | it has been reported that lzd-resistant enterococcus in the gastrointestinal tract of mice colonizes persistently and shows variable minimum inhibitor concentration (mic) values. however, the colonization characteristics of enterococcus with lzd resistance in patients remain elusive. here, we report the case of a patient with recurrent pneumonia due to infection with lzd-resistant enterococcus faecalis strains. the colonization characteristics of the strains isolated from this patient were analy ... | 2015 | 25236388 |
| [development of an enterococcus faecalis periapical biofilm model for in vitro morphological study]. | this study aims to develop and observe a model system of the periapical biofilm structure of enterococcus faecalis (e. faecalis). | 2014 | 25241529 |
| efficacy of a combined nanoparticulate/calcium hydroxide root canal medication on elimination of enterococcus faecalis. | the purpose of this study was to evaluate the efficacy of ca(oh)2 with or without a silver nanoparticle suspension to eliminate enterococcus faecalis from root canals. a total of 66 extracted human single-rooted teeth contaminated with e. faecalis were treated with 10% ca(oh)2 alone, ca(oh)2 with nanosilver or sterile water (as a negative control). samples were obtained with paper points and gates-glidden burs at 1 and 7 days after root canal preparation and the number of colony-forming units (c ... | 2014 | 25244219 |
| in vitro assessment of the recurrent doses of topical gaseous ozone in the removal of enterococcus faecalis biofilms in root canals. | to evaluate the potential antibacterial effect of recurrent doses of topical gaseous ozone on the enterococcus faecalis biofilms0 growth in human root canals in vitro. | 2014 | 25244266 |
| monotreme lactation protein is highly expressed in monotreme milk and provides antimicrobial protection. | monotremes (platypus and echidna) are the descendants of the oldest ancestor of all extant mammals distinguished from other mammals by mode of reproduction. monotremes lay eggs following a short gestation period and after an even briefer incubation period, altricial hatchlings are nourished over a long lactation period with milk secreted by nipple-less mammary patches located on the female's abdomen. milk is the sole source of nutrition and immune protection for the developing young until weanin ... | 2014 | 25245409 |
| transcriptomic response of enterococcus faecalis v583 to low hydrogen peroxide levels. | enterococcus faecalis is a gram-positive commensal bacterium inhabiting the gastrointestinal tracts of human and other mammals, but is also increasingly recognized as an opportunistic human pathogen. oxidative stress is one of the major challenges encountered by enterococci, both in their natural environment and during infection. in this paper, we evaluated the transcriptomic response of e. faecalis to oxidative stress, and showed that transcript abundance was reduced for 93 genes and increased ... | 2015 | 25245959 |
| oligomerization of esculin improves its antibacterial activity and modulates antibiotic resistance. | in this particular study, the antibacterial activity of esculin and oligomer fractions was assessed. mic values of esculin and its oligomer fractions as well as of some antibiotics against gram-positive and gram-negative strains and against escherichia coli multiresistant variants were determined by the standard broth microdilution method. both esculin and oligoesculin fractions exhibited antibacterial effect against reference strains; staphylococcus aureus, enterococcus faecalis, salmonella ent ... | 2015 | 25248728 |
| an abc transporter is required for secretion of peptide sex pheromones in enterococcus faecalis. | enterococci are leading causes of hospital-acquired infection in the united states and continue to develop resistances to new antibiotics. many enterococcus faecalis isolates harbor pheromone-responsive plasmids that mediate horizontal transfer of even large blocks of chromosomal genes, resulting in hospital-adapted strains over a quarter of whose genomes consist of mobile elements. pheromones to which the donor cells respond derive from lipoprotein signal peptides. using a novel bacterial killi ... | 2014 | 25249282 |
| peritonitis in a continuous ambulatory peritoneal dialysis patient by two different species of enterococci: a rare finding. | peritonitis in a continuous ambulatory peritoneal dialysis patient by two different species of enterococci is a rare condition. we report a case of peritonitis from which vancomycin sensitive enterococcus faecalis and vancomycin resistant enterococcusc faecium were isolated. it also emphasizes the effectiveness of linezolid for the treatment of vancomycin resistant enterococcal infection. | 2014 | 25249726 |
| antibacterial power of sodium hypochlorite combined with surfactants and acetic acid. | the aim of this ex vivo study was to compare the antibacterial power of 1% naocl with 1% acetic acid, 5.25% naocl and two commercially available naocl modified with surfactants in bovine root dentin. a total of 120 dentin tubes prepared from intact bovine incisors were infected for 21 days with enterococcus faecalis and randomly divided into six groups as follows: 5.25%naocl; hypoclean; chlor-xtra; 1% naocl with 1% acetic acid; infected dentin tubes (positive control); and sterile dentin tubes ( ... | 2014 | 25250491 |
| antimicrobial efficacy of er,cr:ysgg laser-activated irrigation compared with passive ultrasonic irrigation and rinsendo(®) against intracanal enterococcus faecalis. | the aim of this study was to evaluate the antibacterial efficacy of active irrigation techniques: erbium, chromium: yttrium, scandium, gallium, garnet (er,cr:ysgg) laser-activated irrigation (lai), passive ultrasonic irrigation (pui), rinsendo(®), and conventional syringe irrigation, against intracanal enterococcus faecalis. | 2014 | 25251217 |
| measurement of cytokine levels in cerebrospinal fluid over time in neonatal enterococcal meningitis. | enterococcus faecalis is rarely involved in neonatal meningitis. several studies have indicated that the cytokines related to bacterial infection may induce nerve cell damage; therefore, the cytokine levels in cerebrospinal fluid (csf) could represent a valuable hallmark for rapid recognition of the disease and evaluation of the degree of neurological involvement. we analyzed cytokine levels in the csf of a neonate with e. faecalis meningitis over time. tumor necrosis factor-α (tnf-α) tended to ... | 2014 | 25252071 |
| in vitro antimicrobial activity of an experimental dentifrice based on ricinus communis. | this study evaluated the antimicrobial activity of a ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: staphylococcus aureus, escherichia coli, streptococcus mutans, enterococcus faecalis, candida albicans and candida glabrata. the minimum inhibitory concentration (mic) assay was performed with r. communis in pure oil at 2.5%. only e. coli was not inhibited by r. communis, but the mic (0.0781%) was effective against the other microorganism ... | 2014 | 25252252 |
| intestinal translocation of clinical isolates of vancomycin-resistant enterococcus faecalis and esbl-producing escherichia coli in a rat model of bacterial colonization and liver ischemia/reperfusion injury. | the objectives of this study were to develop a rat model of gastrointestinal colonization with vancomycin-resistant enterococcus faecalis (vre) and extended-spectrum beta-lactamase (esbl)-producing e. coli and to evaluate intestinal translocation to blood and tissues after total and partial hepatic ischemia. methods - we developed a model of rat colonization with vre and esbl-e coli. then we studied four groups of colonized rats: group i (with hepatic pedicle occlusion causing complete liver isc ... | 2014 | 25255079 |
| a widely used in vitro biofilm assay has questionable clinical significance for enterococcal endocarditis. | biofilm formation may play an important role in the pathogenesis of infections caused by enterococcus faecalis, including endocarditis. most biofilm studies use a polystyrene dish assay to quantify biofilm biomass. however, recent studies of e. faecalis strains in tissue and animal models suggest that polystyrene dish results need to be interpreted with caution. we evaluated 158 clinical e. faecalis isolates using a polystyrene dish assay and found variation in biofilm formation, with many isola ... | 2014 | 25255085 |
| terpenoids, flavonoids and caffeic acid derivatives from salvia viridis l. cvar. blue jeans. | three diterpenoids, 1-oxomicrostegiol (1), viroxocin (2), viridoquinone (3), were isolated from the roots of salvia viridis l. cvar. blue jeans. five known diterpenoids, microstegiol (4), 7α-acetoxy-14-hydroxy-8,13-abietadiene-11,12-dione (5; 7-o-acetylhorminone tautomer), 7α,14-dihydroxy-8,13-abietadiene-11,12-dione (6; horminone tautomer), ferruginol and salvinolonyl 12-methyl ether (7) were also found in the roots together with 1-docosyl ferulate (8), and a mixture of 2-(4'-alkoxyphenyl) ethy ... | 2014 | 25256822 |
| antibiofilm efficacy of photosensitizer-functionalized bioactive nanoparticles on multispecies biofilm. | newer disinfection strategies based on antibacterial nanoparticles and photodynamic therapy (pdt) aim to eliminate residual biofilm bacteria during root canal treatment. the aim of the current study was to test the newly developed rose bengal-functionalized chitosan nanoparticles (csrbnps) for their interaction/uptake with monospecies bacteria/biofilm and assess their antibiofilm efficacy on a multispecies biofilm model in vitro. | 2014 | 25260731 |
| in vitro evaluation of antibacterial effect of ah plus incorporated with quaternary ammonium epoxy silicate against enterococcus faecalis. | the purpose of this study was to evaluate the in vitro antibacterial effect of ah plus (dentsply, detrey, konstanz, germany) incorporated with quaternary ammonium epoxy silicate (qaes) against enterococcus faecalis. | 2014 | 25260732 |
| epithelial il-22ra1-mediated fucosylation promotes intestinal colonization resistance to an opportunistic pathogen. | our intestinal microbiota harbors a diverse microbial community, often containing opportunistic bacteria with virulence potential. however, mutualistic host-microbial interactions prevent disease by opportunistic pathogens through poorly understood mechanisms. we show that the epithelial interleukin-22 receptor il-22ra1 protects against lethal citrobacter rodentium infection and chemical-induced colitis by promoting colonization resistance against an intestinal opportunistic bacterium, enterococ ... | 2014 | 25263220 |
| antimicrobial activity of a sodium hypochlorite/etidronic acid irrigant solution. | the aim of this study was to evaluate the antimicrobial activity of a 2.5% sodium hypochlorite (naocl)/9% etidronic acid (hebp) irrigant solution on enterococcus faecalis growing in biofilms and a dentinal tubule infection model. | 2014 | 25266466 |
| a comparative study on sealing ability of mineral trioxide aggregate, calcium enriched cement and bone cement in furcal perforations. | the aim of this study was to compare the bacterial leakage of mineral trioxide aggregate (mta), calcium enriched cement (cem), and bone cement (bc) as repair materials in furcal perforations. | 2014 | 25267149 |
| enterococcus faecalis infection activates phosphatidylinositol 3-kinase signaling to block apoptotic cell death in macrophages. | apoptosis is an intrinsic immune defense mechanism in the host response to microbial infection. not surprisingly, many pathogens have evolved various strategies to manipulate this important pathway to benefit their own survival and dissemination in the host during infection. to our knowledge, no attempts have been made to explore the host cell survival signals modulated by the bacterium enterococcus faecalis. here, we show for the first time that during early stages of infection, internalized en ... | 2014 | 25267834 |
| selection of potential probiotic enterococcus faecium isolated from portuguese fermented food. | four enterococcus faecium strains isolated from fermented products were evaluated for potential use as probiotic strains. in addition to efaafm gene, commonly found in e. faecium food isolates, none of the isolates possessed virulence genes and none had positive reactions for the production of tyramine, histamine, putrescine and cadaverine in the screening medium used. all of these four isolates proved to be resistant to 65 °c. e. faecium 119 did not show antimicrobial activity against any of th ... | 2014 | 25268323 |
| positively charged imidazolium-based ionic liquid-protected silver nanoparticles: a promising disinfectant in root canal treatment. | to synthesize and characterize silver nanoparticles (ag nps) with different surface charges in order to evaluate their cytotoxicity and antibacterial activity in the absence and presence of dentine compared with naocl and chx. | 2015 | 25269666 |
| commensal bacteria directly suppress in vitro degranulation of mast cells in a myd88-independent manner. | the intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. however, the cellular and molecular mechanisms underlying these effects remain to be determined. here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a myd88-independent manner. i ... | 2014 | 25273132 |
| use of selected lactic acid bacteria in the eradication of helicobacter pylori infection. | helicobacter pylori is among the major pathogenic bacteria that cause chronic gastritis and peptic ulcer disease and is related to the development of gastric cancer. several chemicals, including antibiotics, have been used to eradicate h. pylori; however, they do not always curb the infection. ten representative type strains of lactic acid bacteria (lab) were screened for antagonism toward h. pylori via inhibition of urease activity. strains inhibiting the binding of h. pylori to human gastric c ... | 2014 | 25277407 |
| evolution of the resistance to antibiotics of bacteria involved in urinary tract infections: a 7-year surveillance study. | we conducted a retrospective analysis on the identification and antibiogram of all bacteria isolated from urine samples with microbiological confirmation of urinary tract infection (uti) in a spanish reference hospital over a 7-year period. | 2014 | 25278389 |
| biohybrid polymer-antimicrobial peptide medium against enterococcus faecalis. | antimicrobial peptides (amps) are conserved evolutionary components of the innate immune system that are being tested as alternatives to antibiotics. slow release of amps using biodegradable polymers can be advantageous in maintaining high peptide levels for topical treatment, especially in the oral environment in which dosage retention is challenged by drug dilution with saliva flow and by drug inactivation by salivary enzymatic activity. enterococcus faecalis is a multidrug resistant nosocomia ... | 2014 | 25279943 |
| comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis: an in vitro study. | the purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (ca[ocl]2) and sodium hypochlorite (naocl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with enterococcus faecalis. | 2014 | 25282371 |
| new antibacterial xanthone from the marine sponge-derived micrococcus sp. eg45. | microluside a [4 (19-para-hydroxy benzoyloxy-o-β-d-cellobiosyl), 5 (30-para-hydroxy benzoyloxy-o-β-d-glucopyranosyl) xanthone (1)] is a unique o-glycosylated disubstituted xanthone isolated from the broth culture of micrococcus sp. eg45 cultivated from the red sea sponge spheciospongia vagabunda. the structure of microluside a was determined by 1d- and 2d-nmr techniques as well as high resolution tandem mass spectrometry. the antimicrobial activity evaluation showed that 1 exhibited antibacteria ... | 2014 | 25283555 |
| a comparative evaluation of antibacterial efficacy of 'activ points' and 'combi points' as intra-canal medicaments against enterococcus faecalis: an ex vivo study. | enterococcus faecalis has been found to be one of the most predominant bacterial species associated with failed endodontic cases. the ability of this microbe to form biofilms; penetrate into dentinal tubules; survival in low ph, high salinity and high temperatures and resistance to many intracanal medicaments, makes it one of the most resistant pathogen of all the root canal flora. studies have shown that chlorhexidine is relatively more effective against e. faecalis as compared to other intra c ... | 2014 | 25284521 |
| mapping of the modular closthioamide architecture reveals crucial motifs of polythioamide antibiotics. | closthioamide, the first known secondary metabolite from an anaerobic microorganism (clostridium cellulolyticum), represents a highly potent antibiotic that is active against methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus faecalis (vre) at nanomolar concentrations. to unveil structure-activity relationships of the unusual polythioamide natural product we have designed a synthetic grid to access analogues with altered terminal aromatic moieties, diverse p ... | 2014 | 25284750 |
| effects of storage temperature on tyramine production by enterococcus faecalis r612z1 in water-boiled salted ducks. | tyramine production by enterococcus faecalis r612z1 in water-boiled salted ducks was evaluated during storage at different temperatures. the results showed that e. faecalis r612z1 could produce tyramine in meat samples when the storage temperature was no less than 4°c. the e. faecalis r612z1 counts of the meat samples reached 10(8) cfu/g on day 7 at 4°c and on day 4 at 10°c. however, the tyramine content of the meat samples stored at 10°c increased to 23.73 μg/g (on day 10), which was greater th ... | 2014 | 25285502 |
| infantile subdural empyema: the role of brain sonography and percutaneous subdural tapping in a resource-challenged region. | this study explored the outcome of children with patent anterior fontanelles who were treated with trans-fontanelle ultrasound scan (tfuss), which is more affordable and available than ct scan and mri in the diagnosis of childhood intracranial pathologies and treatment of subdural empyema, in developing countries. | 2014 | 25288836 |
| enterococcus--virulence and susceptibility to photodynamic therapy of clinical isolates from lower silesia, poland. | a collection of 400 enterococcal isolates from clinical samples of hospitalized patients were studied for their virulence traits according to the isolation site. formation of biofilm and production of dnase, hemolysin, lipase, and gelatinase were characterized. biofilms of selected strains were examined for their susceptibility to antimicrobial photodynamic therapy (apdt). | 2014 | 25290581 |
| complete genome assembly of enterococcus faecalis 29212, a laboratory reference strain. | enterococcus faecalis is a nonmotile gram-positive coccus, found both as a commensal organism in healthy humans and animals and as a causative agent of multiple diseases, in particular endocarditis. we sequenced the genome of e. faecalis atcc 29212, a commonly used reference strain in laboratory studies, to complete "finished" annotated assembly (3 mb). | 2014 | 25291775 |
| prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients. | mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ... | 2014 | 25292217 |
| antimicrobial activity, toxicity and selectivity index of two biflavonoids and a flavone isolated from podocarpus henkelii (podocarpaceae) leaves. | different parts of podocarpus henkelii have been used in many cultures around the world to treat ailments such as cholera, stomach diseases, rheumatism, cancer, canine distemper in dogs and gall sickness in cattle. the aim of this study was to evaluate the biological activity and toxicity of isolated compounds from podocarpus henkelii after an earlier study indicated a promising activity in crude extracts against viral pathogens of veterinary importance. | 2014 | 25293523 |
| large vegetation in a 60-year-old man with enterococcus faecalis cardiac implantable electronic device infection. | 2014 | 25293687 | |
| in vitro activity of oritavancin against gram-positive pathogens isolated in canadian hospital laboratories from 2011 to 2013. | gram-positive pathogens isolated in 15 canadian hospital laboratories between 2011 and 2013 were tested for susceptibility to oritavancin and comparative antimicrobial agents using the clinical and laboratory standards institute broth microdilution method. oritavancin demonstrated in vitro activity equivalent to, or more potent than, vancomycin, daptomycin, linezolid, and tigecycline against the isolates of methicillin-susceptible staphylococcus aureus (n=1460; oritavancin mic90, 0.06 μg/ml; 99. ... | 2014 | 25294303 |
| a novel role for d-alanylation of lipoteichoic acid of enterococcus faecalis in urinary tract infection. | enterococci are the third most common cause of healthcare-associated infections, which include urinary tract infections, bacteremia and endocarditis. cell-surface structures such as lipoteichoic acid (lta) have been poorly examined in e. faecalis, especially with respect to urinary tract infections (utis). the dlt operon is responsible for the d-alanylation of lta and includes the gene dlta, which encodes the d-alanyl carrier protein ligase (dcl). the involvement of lta in uti infection by e. fa ... | 2014 | 25296179 |
| antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against enterococcus faecalis biofilm. | the aim of the present study was to compare the antibacterial effect of calcium hydroxide (ch), triantibiotic mixture (tam), and ch in combination with chlorhexidine (chx), sodium hypochlorite (naocl) or colchicine (col) against enterococcus faecalis (e. faecalis) in surface and deep dentinal tubules. seventy five fresh single-rooted human teeth were infected and divided into five experimental groups (n=15). the experimental groups were treated with ch+distilled water, ch+chx, ch+naocl, ch+col+d ... | 2014 | 25297855 |
| viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy. | several disinfection techniques have been recently introduced with the main objective of improving root canal disinfection in the inaccessible areas of the root canal system. this in vitro study was done to evaluate the antimicrobial effect and viability of enterococcus faecalis biofilms using conventional irrigation, endoactivator (dentsply, tulsa dental, usa), diode laser irradiation and photon-initiated photoacoustic streaming (pips). | 2014 | 25298645 |
| chemical constituent and antimicrobial effect of essential oil from myrtus communis leaves on microorganisms involved in persistent endodontic infection compared to two common endodontic irrigants: an in vitro study. | persistent infections of human root canals play a fundamental role in the failure of endodontic treatment. the purpose of this study is to determine the chemical composition of myrtus communis (m. communis) essential oil and to assess its antimicrobial activity against enterococcus faecalis, staphylococcus aureus and candida albicans compared to that of sodium hypochlorite (naocl) and chlorhexidine (chx). | 2014 | 25298646 |
| a delicate balance: maintaining mutualism to prevent disease. | the intestinal microbial ecosystem is complex, and few of the principles that contribute to homeostasis in health are well understood. pham et al. (2014) show that a network including the epithelial interleukin-22 receptor protects against infection with the opportunistic pathogen enterococcus faecalis through promotion of host-microbiota mutualism. | 2014 | 25299326 |
| comparative evaluation of antimicrobial activity of two root canal sealers: mta fillapex and ah 26. | even after a complete root canal therapy, reinfection may occur as a result of incomplete seal and activation of residual bacteria. thus, antimicrobial activity is an important characteristic of root canal sealers. mta fillapex is a newly developed mta-based sealer. it has been subjected to various studies evaluating its physical and chemical properties. in this study, we aimed to compare the antimicrobial activity of this sealer to the commonly known widely accepted sealer, ah26. | 2014 | 25299361 |
| virulence factors genes in enterococci isolated from beavers (castor fiber). | only limited information exists concerning the microbiota in beaver (castor fiber). this study has been focused on the virulence factors genes detection in enterococci from beavers. in general, animals are not affected by enterococcal infections, but they can be a reservoir of, e.g. pathogenic strains. moreover, detection of virulence factors genes in enterococci from beavers was never tested before. free-living beavers (12), male and female (age 4-5 years) were caught in the north-east part of ... | 2015 | 25300356 |
| comparison of the antibacterial efficacy of manuka honey against e.faecalis and e.coli - an in vitro study. | to compare the antibacterial efficacy of manuka honey against e.faecalis and e.coli. | 2014 | 25302263 |
| ceftobiprole and ampicillin increase daptomycin susceptibility of daptomycin-susceptible and -resistant vre. | the synergistic combination of daptomycin plus ampicillin has proven to be effective against vre including daptomycin-non-susceptible strains. ceftobiprole is a cephalosporin with broad binding affinity for enterococcal pbp subtypes including pbp5. given the synergy between β-lactams and daptomycin against vre, it was of interest to determine whether ceftobiprole offered any synergistic advantage with daptomycin compared with ampicillin. | 2015 | 25304643 |
| novel in vitro methodology for induction of enterococcus faecalis biofilm on apical resorption areas. | teeth with periapical lesion usually present external root resorption around the apical foramen. these areas facilitate adhesion and co-aggregation of microorganisms developing biofilms. up to the present moment, there is no methodology in the literature that enables the in vitro evaluation of endodontic irrigants and intracanal dressings on biofilms located in apical external root resorptions of human teeth. | 2014 | 25307923 |
| anaerobic bacteria grow within candida albicans biofilms and induce biofilm formation in suspension cultures. | the human microbiome contains diverse microorganisms, which share and compete for the same environmental niches. a major microbial growth form in the human body is the biofilm state, where tightly packed bacterial, archaeal, and fungal cells must cooperate and/or compete for resources in order to survive. we examined mixed biofilms composed of the major fungal species of the gut microbiome, candida albicans, and each of five prevalent bacterial gastrointestinal inhabitants: bacteroides fragilis, ... | 2014 | 25308076 |
| antibacterial and antibiofilm activities of polysaccharides, essential oil, and fatty oil extracted from laurus nobilis growing in lebanon. | to evaluate the antibacterial activity of the extracts of laurus nobilis against three gram-positive bacteria (staphylococcus aureus atcc 25923, enterococcus faecalis atcc 29212 and staphylococcus epidermidis cip 444) and two gram-negative bacteria (escherichia coli atcc 35218 and pseudomonas aeruginosa atcc 27853). also, the antibiofilm activity has been investigated against the biofilm produced by staphylococcus epidermidis cip 444. | 2014 | 25312182 |
| enterococcus faecalis zinc-responsive proteins mediate bacterial defence against zinc overload, lysozyme and oxidative stress. | two enterococcus faecalis genes encoding the p-type atpase ef1400 and the putative sapb protein ef0759 were previously shown to be strongly upregulated in the presence of high concentrations of zinc. in the present work, we showed that a zn(2+)-responsive dna-binding motif (zim) is present in the promoter regions of these genes. both proteins were further studied with respect to their involvement in zinc homeostasis and invasion of the host. ef0759 contributed to intramacrophage survival by an a ... | 2014 | 25312746 |
| the type i toxin-antitoxin par locus from enterococcus faecalis plasmid pad1: rna regulation by both cis- and trans-acting elements. | the pad1 par determinant was the first type i toxin-antitoxin system identified in gram-positive bacteria and has recently been shown to be the prototype of a family of loci that is widespread in these organisms. all family members have (i) convergently transcribed toxin message and regulatory rnas, (ii) three non-contiguous complementary regions for potential interaction, and (iii) intramolecular structures within the toxin message that modulate translation and transcript stability. therefore, ... | 2015 | 25312777 |
| optimizing therapy for vancomycin-resistant enterococcal bacteremia in children. | uncertainties exist regarding the optimal treatment for vancomycin-resistant enterococcal (vre) bloodstream infections, particularly in settings in which ampicillin cannot be used. | 2014 | 25313503 |
| pyranoside-into-furanoside rearrangement: new reaction in carbohydrate chemistry and its application in oligosaccharide synthesis. | great interest in natural furanoside-containing compounds has challenged the development of preparative methods for their synthesis. herein a novel reaction in carbohydrate chemistry, namely a pyranoside-into-furanoside (pif) rearrangement permitting the transformation of selectively o-substituted pyranosides into the corresponding furanosides is reported. the discovered process includes acid-promoted sulfation accompanied by rearrangement of the pyranoside ring into a furanoside ring followed b ... | 2014 | 25319316 |
| assessing outcomes of adult oncology patients treated with linezolid versus daptomycin for bacteremia due to vancomycin-resistant enterococcus. | the incidence and severity of vancomycin-resistant enterococcus blood stream infections continue to rise and is a significant burden in the healthcare setting. literature thus far is minimal regarding treatment outcomes in patients with malignancy and vancomycin-resistant enterococcus bacteremia. appropriate antibiotic selection is vital to treatment success due to high rates of resistance, limited antimicrobials and mortality in this patient population. we conducted this study to determine whet ... | 2016 | 25326010 |
| distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using rna- and dna-based quantitative pcr assays. | the identification of fecal pollution sources is commonly carried out using dna-based methods. however, there is evidence that dna can be associated with dead cells or present as "naked dna" in the environment. furthermore, it has been shown that rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) assays can be more sensitive than rrna gene-based qpcr assays since metabolically active cells usually contain higher numbers of ribosomes than quiescent cells. to this end, we compared the ... | 2015 | 25326295 |
| acarbose treatment affects the serum levels of inflammatory cytokines and the gut content of bifidobacteria in chinese patients with type 2 diabetes mellitus. | the effects of acarbose add-on therapy on gut microbiota and inflammatory cytokines were investigated in chinese patients with type 2 diabetes mellitus (dm). | 2015 | 25327485 |
| [clinical and bacteriological characteristic of infectious purulent complications in patients after related renal transplantation]. | we investigated the frequency and characteristics of infectious purulent and non-infectious complications in living related renal transplant recipients in early postoperative period. it was identified the prevalent microorganisms in urinary tract infections and its antibiotic sensitivity: pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, citrobacter freundii, staphylococcus epidermidis, enterococcus faecalis, candida albicans. 182 strains of bacteria and cand ... | 2014 | 25327674 |
| central line associated blood stream infection rate after intervention and comparing outcome with national healthcare safety network and international nosocomial infection control consortium data. | benchmarking of central line associated blood stream infection (clabsi) rates remains a problem in developing countries due to the variations in surveillance practices and/or infection risk as non-availability of national data. | 2014 | 25328774 |
| oxidative stress enhances cephalosporin resistance of enterococcus faecalis through activation of a two-component signaling system. | enterococcus faecalis is a low-gc gram-positive bacterium, a normal resident of the gastrointestinal (gi) tract, and an important hospital-acquired pathogen. an important risk factor for hospital-acquired enterococcal infections is prior therapy with broad-spectrum cephalosporins, antibiotics that impair cell wall biosynthesis by inhibiting peptidoglycan cross-linking. enterococci are intrinsically resistant to cephalosporins; however, environmental factors that modulate cephalosporin resistance ... | 2015 | 25331701 |
| synthetic teichoic acid conjugate vaccine against nosocomial gram-positive bacteria. | lipoteichoic acids (lta) are amphiphilic polymers that are important constituents of the cell wall of many gram-positive bacteria. the chemical structures of lta vary among organisms, albeit in the majority of gram-positive bacteria the ltas feature a common poly-1,3-(glycerolphosphate) backbone. previously, the specificity of opsonic antibodies for this backbone present in some gram-positive bacteria has been demonstrated, suggesting that this minimal structure may be sufficient for vaccine dev ... | 2014 | 25333799 |