Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| listeria monocytogenes engineered to activate the nlrc4 inflammasome are severely attenuated and are poor inducers of protective immunity. | inflammasomes are intracellular multiprotein signaling complexes that activate caspase-1, leading to the cleavage and secretion of il-1ß and il-18, and ultimately host cell death. inflammasome activation is a common cellular response to infection; however, the consequences of inflammasome activation during acute infection and in the development of long-term protective immunity is not well understood. to investigate the role of the inflammasome in vivo, we engineered a strain of listeria monocyto ... | 2011 | 21746921 |
| sublethal triclosan exposure decreases susceptibility to gentamicin and other aminoglycosides in listeria monocytogenes. | the human foodborne pathogen listeria monocytogenes is capable of persisting in food processing plants despite cleaning and sanitation and is likely exposed to sublethal biocide concentrations. this could potentially affect susceptibility of the bacterium to biocides and other antimicrobial agents. the purpose of the present study was to determine if sublethal biocide concentrations affected antibiotic susceptibility in l. monocytogenes. exposure of l. monocytogenes strains egd and n53-1 to subl ... | 2011 | 21746948 |
| worldwide distribution of major clones of listeria monocytogenes. | listeria monocytogenes is worldwide a pathogen, but the geographic distribution of clones remains largely unknown. genotyping of 300 isolates from the 5 continents and diverse sources showed the existence of few prevalent and globally distributed clones, some of which include previously described epidemic clones. cosmopolitan distribution indicates the need for genotyping standardization. | 2011 | 21749783 |
| comparison of two aflp methods and pfge using strains of listeria monocytogenes isolated from environmental and food samples obtained from piedmont, italy. | listeria monocytogenes ranks among the most frequent causes of death due to foodborne illness (20-30% case fatality rate). discriminative subtyping methods are important to detect the relatedness of isolates and verify epidemiologic associations. aflp analysis is a dna fingerprinting technique based on the selective amplification of genomic restriction fragments. in this study, two aflp methods and pfge were compared in regard to discriminatory power, typeability and concordance. a total of 103 ... | 2011 | 21752482 |
| rhombencephalitis caused by listeria monocytogenes with striking involvement of trigeminal nerve on mr imaging. | 2011 | 21755145 | |
| listeria monocytogenes cell invasion: a new role for cofilin in co-ordinating actin dynamics and membrane lipids. | actin reorganization, mediated by the actin dynamizing protein cofilin, is essential for host cell invasion by the intracellular pathogenic bacterium listeria monocytogenes. during invasion, the inlb bacterial surface ligands closely interact with host cell met receptors to induce phagocytosis. in this issue of molecular microbiology, han et al. (pp. ••-••) clearly demonstrate that phospholipase d (pld)-dependent production of membrane phosphatidic acid is required for invasion. they further sho ... | 2011 | 21762221 |
| specific detection of viable listeria monocytogenes in spanish wastewater treatment plants by fluorescent in situ hybridization and pcr. | listeria monocytogenes detection in wastewater can be difficult because of the large amount of background microbiota and the presence of viable but non-culturable forms in this environment. the aim of this study was to evaluate a fluorescent in situ hybridization (fish) assay combined with direct viable count (dvc) method for detecting viable l. monocytogenes in wastewater samples, as an alternative to conventional culture methods. 16s rrna sequence data were used to design a specific oligonucle ... | 2011 | 21762946 |
| cellular accumulation of fluoroquinolones is not predictive of their intracellular activity: studies with gemifloxacin, moxifloxacin and ciprofloxacin in a pharmacokinetic/pharmacodynamic model of uninfected and infected macrophages. | fluoroquinolones enter eukaryotic cells but the correlation between cellular accumulation and activity remains poorly established. gemifloxacin is known to accumulate to a larger extent than most other fluoroquinolones in tissues. using murine j774 macrophages and human thp-1 monocytes, we show that gemifloxacin accumulates more than ciprofloxacin and even moxifloxacin. whilst showing indistinguishable kinetics of accumulation in j774 macrophages, gemifloxacin was released at an approximately tw ... | 2011 | 21764262 |
| monte carlo simulation of parameter confidence intervals for non-linear regression analysis of biological data using microsoft excel. | this study describes a method to obtain parameter confidence intervals from the fitting of non-linear functions to experimental data, using the solver and analysis toolpak add-in of the microsoft excel spreadsheet. previously we have shown that excel can fit complex multiple functions to biological data, obtaining values equivalent to those returned by more specialized statistical or mathematical software. however, a disadvantage of using the excel method was the inability to return confidence i ... | 2011 | 21764476 |
| physiological and transcriptional characterisation of persistent and non-persistent listeria monocytogenes isolates. | this study aimed to characterise physiological differences between persistent and presumed non-persistent listeria monocytogenes strains isolated at processing facilities, and to investigate the molecular basis for this by transcriptomic sequencing. full metabolic profiles of two strains, one persistent and one non-persistent, were initially obtained using biolog's phenotype microarray™ (pm) technology. based on these results in which major differences to selected antimicrobial agents were detec ... | 2011 | 21764947 |
| a multiparameter viability assay for stress profiling applied to the food pathogen listeria monocytogenes f2365. | a novel generic approach for stress profiling was applied to listeria monocytogenes strain f2365. this food-borne pathogen was exposed to gradients of five different stresses of increasing intensity, typically ranging from moderate to lethal conditions. the stress factors included heat, acidic ph, a detergent disinfectant, an oxidant, and hyperosmotic conditions. in addition to cfu's and lag time, five different molecular viability parameters were measured by fluorescence-based assays, including ... | 2011 | 21764948 |
| transcriptomic response of listeria monocytogenes during transition to the long-term-survival phase. | listeria monocytogenes can change its cellular morphology from bacilli to cocci during the transition to the long-term-survival (lts) phase. the lts cells demonstrated increased baro- and thermotolerance than their vegetative counterparts. so far, the underlying mechanisms that trigger this morphological and physiological transition remain largely unknown. in this study, we compared the transcriptomic profiles of a l. monocytogenes serotype 4b strain f2365 at different growth stages in tryptic s ... | 2011 | 21764970 |
| vascular endograft infection with listeria monocytogenes reated with surgical debridement but without graft removal. | the awareness of listeria monocytogenes as a pathogen in meningitis and bacteremia in immunosuppressed patients is high. we report a case of vascular graft infection due to listeria monocytogenes as an example of a less well-known manifestation of listeriosis and focus on the possible treatment procedures emphasizing a management with surgical debridement but preservation of the endograft, in contrast to the gold standard treatment of vascular graft infections which consists of a removal of the ... | 2011 | 21765844 |
| listeria monocytogenes-induced bacterial peritonitis caused by contaminated cheese in a patient with haemochromatosis. | infections with listeria monocytogenes can present clinically with a wide range of different organ manifestations such as gastroenteritis, meningoencephalitis or osteomyelitis, posing a serious threat, particularly to immunocompromised patients. we present the case of a 76-year-old female patient with advanced liver disease due to underlying haemochromatosis, who was admitted to the hospital with increasing abdominal pain. she was diagnosed with spontaneous bacterial peritonitis caused by infect ... | 2011 | 21766262 |
| a complex lipoate utilization pathway in listeria monocytogenes. | although a complete pathway of lipoic acid metabolism has been established in escherichia coli, lipoic acid metabolism in other bacteria is more complex and incompletely understood. listeria monocytogenes has been shown to utilize two lipoate-protein ligases for lipoic acid scavenging whereas only one of the ligases can function in utilization of host-derived lipoic acid modified peptides. we report that lipoic acid scavenging requires not only ligation of lipoic acid, but also a lipoyl relay pa ... | 2011 | 21768091 |
| mutations of the listeria monocytogenes peptidoglycan n-deacetylase and o-acetylase result in enhanced lysozyme sensitivity, bacteriolysis and hyper-induction of innate immune pathways. | listeria monocytogenes is a gram-positive intracellular pathogen that is naturally resistant to lysozyme. recently, it was shown that peptidoglycan modification by n-deacetylation or o-acetylation confers resistance to lysozyme in various gram-positive bacteria including l. monocytogenes. l. monocytogenes peptidoglycan is deacetylated by the action of n-acetylglucosamine deacetylase (pgd) and acetylated by an o-acetylmuramic acid transferase (oat). we characterized pgd-minus, oat-minus and doubl ... | 2011 | 21768286 |
| [antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760]. | antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ... | 2011 | 21780664 |
| a glance at listeria and salmonella cell invasion: different strategies to promote host actin polymerization. | the facultative intracellular bacterial pathogens listeria monocytogenes and salmonella enterica have evolved multiple strategies to invade a large panel of mammalian cells. these pathogens use the host cell actin system for invasion and became a paradigm for the study of host-pathogen interactions and bacterial adaptation to mammalian hosts. the key signaling component that these pathogens use to orchestrate actin remodeling is the arp2/3 complex, which is related to polymerization of actin fil ... | 2011 | 21783413 |
| the quorum sensing luxs gene is induced in lactobacillus acidophilus ncfm in response to listeria monocytogenes. | the luxs gene involved in quorum sensing has been shown to control different behaviour of probiotic lactobacilli. in this study we investigated if luxs in lactobacillus acidophilus ncfm was up-regulated in response to listeria monocytogenes egd-e. the two bacterial strains were grown in mono- and co-culture and the growth of both bacteria and the transcriptional level of luxs in l. acidophilus cells were monitored. contrary to l. acidophilus, the growth of l. monocytogenes was significantly affe ... | 2011 | 21784546 |
| the microrna mir-29 controls innate and adaptive immune responses to intracellular bacterial infection by targeting interferon-? | interferon-? (ifn-?) has a critical role in immune responses to intracellular bacterial infection. micrornas (mirnas) are important in the regulation of innate and adaptive immunity. however, whether mirnas can directly target ifn-? and regulate ifn-? production post-transcriptionally remains unknown. here we show that infection of mice with listeria monocytogenes or mycobacterium bovis bacillus calmette-guérin (bcg) downregulated mir-29 expression in ifn-?-producing natural killer cells, cd4(+) ... | 2011 | 21785411 |
| immune unresponsiveness to secondary heterologous bacterial infection after sepsis induction is trail dependent. | sepsis is the leading cause of death in most intensive care units, and patients who survive the hyperinflammation that develops early during sepsis later display severely compromised immunity. not only is there apoptosis of lymphoid and myeloid cells during sepsis that depletes these critical cellular components of the immune system, but also the remaining immune cells show decreased function. using a cecal-ligation and puncture (clp) model to induce intra-abdominal polymicrobial peritonitis, we ... | 2011 | 21788440 |
| positive selecting cell type determines the phenotype of mhc class ib-restricted cd8+ t cells. | several studies have demonstrated an apparent link between positive selection on hematopoietic cells (hcs) and an "innate" t-cell phenotype. whereas conventional cd8(+) t cells are primarily selected on thymic epithelial cells (tecs) and certain innate t cells are exclusively selected on hcs, mhc class ib-restricted cd8(+) t cells appear to be selected on both tecs and hcs. however, whether tec- and hc-selected t cells represent distinct lineages or whether the same t-cell precursors have the ca ... | 2011 | 21788511 |
| the cell wall binding domain of listeria bacteriophage endolysin plyp35 recognizes terminal glcnac residues in cell wall teichoic acid. | the cell wall binding domains (cbd) of bacteriophage endolysins target the enzymes to their substrate in the bacterial peptidoglycan with extraordinary specificity. despite strong interest in these enzymes as novel antimicrobials, little is known regarding their interaction with the bacterial wall and their binding ligands. we investigated the interaction of listeria phage endolysin plyp35 with carbohydrate residues present in the teichoic acid polymers on the peptidoglycan. biochemical and gene ... | 2011 | 21790805 |
| an in silico dna vaccine against listeria monocytogenes. | listeria monocytogenes causes listeriosis with mortality rate >20%. listeriolysin-o (llo), a pore-forming hemolysin, belongs to the family of cholesterol-dependent toxins (cdtx) and plays roles in the pathogenicity. in this study bioinformatic analyses were carried out on llo sequence as a major immunodominant listerial antigen toward designing a dna vaccine stimulating cytotoxic t-lymphocytes (ctls). mouse and human constructs were designed based on predicted t cell epitopes and mhc class i bin ... | 2011 | 21791233 |
| prior frozen storage enhances the effect of edible coatings against listeria monocytogenes on cold-smoked salmon during subsequent refrigerated storage. | aims: listeria monocytogenes is a major safety concern for ready-to-eat foods. the overall objective of this study was to investigate whether prior frozen storage could enhance the efficacy of edible coatings against l. monocytogenes on cold-smoked salmon during subsequent refrigerated storage. methods and results: a formulation consisting of sodium lactate (sl, 1.2-2.4%) and sodium diacetate (sd, 0.125-0.25%) or 2.5% opti.form (a commercial formulation of sl and sd) were incorporated into eac ... | 2011 | 21794035 |
| [biologic therapy and infections]. | biologic therapy is an important therapeutic arsenal in rheumatic diseases. anti-tnf therapies affect host defenses against infections, since tnf mediates inflammation and modulates cellular immune responses. cases of tuberculosis have been observed in patients treated with tnf antagonists, mainly due to the presence of latent or "dormant" tuberculosis infection (ltb1). other microorganisms responsible for the infectious complications associated with biologic therapy are generally intracellular ... | 2008 | 21794348 |
| effectiveness of zataria multiflora boiss essential oil and grape seed extract impregnated chitosan film on ready-to-eat mortadella-type sausages during refrigerated storage. | background: the effectiveness of chitosan films containing zataria multiflora boiss essential oil (zeo) (5 and 10 g kg(-1) ) and grape seed extract (gse) (10 g kg(-1) ) on lipid oxidation and microbial (lactic acid bacteria, aerobic mesophiles and inoculated listeria monocytogenes) characteristics of mortadella sausage at 4 °c for 21 days was evaluated. the release of total phenolics (tps) into sausage was also assessed. results: all films exhibited antibacterial activity against l. monocytogene ... | 2011 | 21796636 |
| aberrant immune responses in a mouse with behavioral disorders. | btbr t+tf/j (btbr) mice have recently been reported to have behaviors that resemble those of autistic individuals, in that this strain has impairments in social interactions and a restricted repetitive and stereotyped pattern of behaviors. since immune responses, including autoimmune responses, are known to affect behavior, and individuals with autism have aberrant immune activities, we evaluated the immune system of btbr mice, and compared their immunity and degree of neuroinflammation with tha ... | 2011 | 21799730 |
| [thermal inactivation model of listeria monocytogenes in ground beef]. | to set up suitable kinetic models for the evaluation of heat inactivation rate of listeria monocytogenes in ground beef. | 2011 | 21800632 |
| microbial inactivation in cloudy apple juice by multi-frequency dynashock power ultrasound. | the study determined the efficacy of dynashock wave power ultrasound as an alternative processing technique for apple juice against a number of pathogenic and spoilage microorganisms. the effects of several implicit, intrinsic and extrinsic properties on the dynashock wave inactivation of the microorganisms were also investigated. results showed that acid adaptation increased the resistance of escherichia coli o157:h7 and salmonella spp. but decreased that of listeria monocytogenes. spoilage yea ... | 2011 | 21802974 |
| biocontrol of listeria monocytogenes and e. coli o157:h7 in meat using phage immobilized on modified cellulose membranes. | the ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. the range of applications of bacteriophage can be extended by their immobilization on inert surfaces. a novel method for the oriented immobilization of bacteriophage has been developed. the method was based on charge differences between the bacteriophage head, which exhibits an overall net negative charge, and the tail fibers, which possess an overall net positive charge. hence ... | 2011 | 21803890 |
| structure-based modification of a clostridium difficile targeting endolysin affects activity and host range. | endolysin cd27l causes cell lysis of the pathogen c. difficile, a major cause of nosocomial infection. we report a structural and functional analysis of the catalytic activity of cd27l against c. difficile and other bacterial strains. we show that truncation of the endolysin to the n-terminal domain cd27l(1-179) gave an increased lytic activity against cells of c. difficile, while the c-terminal region cd27l(180-270) failed to produce lysis. cd27l(1-179) also has increased activity against other ... | 2011 | 21803993 |
| the metalloprotease of listeria monocytogenes is regulated by ph. | listeria monocytogenes is an intracytosolic bacterial pathogen. among the factors contributing to escape from vacuoles are a phosphatidylcholine phospholipase c (pc-plc) and a metalloprotease (mpl). both enzymes are translocated across the bacterial membrane as inactive proproteins, whose propeptides serve in part to maintain them bacterium-associated. we have shown that pc-plc maturation is regulated by mpl and ph, and that mpl maturation occurs by autocatalysis. in this study, we tested the hy ... | 2011 | 21803995 |
| autocrine il-2 is required for secondary population expansion of cd8(+) memory t cells. | two competing theories have been put forward to explain the role of cd4(+) t cells in priming cd8(+) memory t cells: one proposes paracrine secretion of interleukin 2 (il-2); the other proposes the activation of antigen-presenting cells (apcs) via the costimulatory molecule cd40 and its ligand cd40l. we investigated the requirement for il-2 by the relevant three cell types in vivo and found that cd8(+) t cells, rather than cd4(+) t cells or dendritic cells (dcs), produced the il-2 necessary for ... | 2011 | 21804558 |
| amplified fragment length polymorphism analysis of listeria monocytogenes by experion™ automated microfluidic electrophoresis. | fifty listeria monocytogenes strains were genotyped by saflp and pcr products were separated by agarose gel and automated chip-based microfluidic electrophoresis. a high congruency of results was observed comparing the two techniques, although for some cultures a better separation of saflp fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of l. monocytogenes, requiring lower volumes of samples and reducing ... | 2011 | 21807035 |
| clemastine causes immune suppression through inhibition of extracellular signal-regulated kinase-dependent proinflammatory cytokines. | background: antihistamines are considered safe and used worldwide against allergy, pruritus, nausea, and cough and as sleeping aids. nonetheless, a growing number of reports suggest that antihistamines also have immunoregulatory functions. objective: we examined the extent and by what potential mechanisms histamine-1-receptor (h1r) antagonists exert immune suppressive effects. methods: immune suppression by antihistamines and immunosuppressants was tested in mice infected with listeria monocytog ... | 2011 | 21807405 |
| microbial control of food-related surfaces: na-chlorophyllin-based photosensitization. | the aim of this study was to evaluate efficiency of photosensitization as surface sanitation alternative using model systems when food pathogens, their spores and biofilms were attached to the food-related surface (polyolefine). in addition it was important to compare antibacterial efficiency of na-chlorophyllin (na-chl)-based photosensitization with conventional sanitizers. obtained results indicate that bacilluscereus atcc 12826 and listeriamonocytogenes atcc 7644 as well as their thermoresist ... | 2011 | 21807530 |
| early onset of neonatal listeriosis. | background l. monocytogenes infection is not commonly reported in china. increasing the awareness of neonatal listeriosis is necessary. methods six cases of neonatal listeriosis were diagnosed in the neonatal intensive care unit at beijing obstetrics & gynecology hospital in 2008. we reviewed the clinical features, laboratory findings, timing of treatment, medications used, and the history of the mothers during pregnancy. result all six cases were preterm infants. blood cultures were positive fo ... | 2011 | 21810151 |
| foxp3+ regulatory t cells impede the priming of protective cd8+ t cells. | t cell activation is controlled by incompletely defined opposing stimulation and suppression signals that together sustain the balance between optimal host defense against infection and peripheral tolerance. in this article, we explore the impacts of foxp3(+) regulatory t cell (treg) suppression in priming ag-specific t cell activation under conditions of noninfection and infection. we find the transient ablation of foxp3(+) tregs unleashes the robust expansion and activation of peptide-stimulat ... | 2011 | 21810602 |
| intrinsic tween 20 improves release and antilisterial properties of co-encapsulated nisin and thymol. | antimicrobial delivery systems have been proposed as potential solutions to improve effectiveness of antimicrobials in food matrices by shielding antimicrobials from contacting food matrix components and releasing them continuously. in this work, spray-dried capsules were produced from zein solutions with same concentrations of nisin and thymol but with varying tween 20 contents for characterization of release kinetics of antimicrobials and antilisterial properties. at intermediate levels of twe ... | 2011 | 21812480 |
| mpys is required for ifn response factor 3 activation and type i ifn production in the response of cultured phagocytes to bacterial second messengers cyclic-di-amp and cyclic-di-gmp. | cyclic-di-gmp and cyclic-di-amp are second messengers produced by bacteria and influence bacterial cell survival, differentiation, colonization, biofilm formation, virulence, and bacteria-host interactions. in this study, we show that in both raw264.7 macrophage cells and primary bone marrow-derived macrophages, the production of ifn-β and il-6, but not tnf, in response to cyclic-di-amp and cyclic-di-gmp requires mpys (also known as sting, mita, and tmem173). furthermore, expression of mpys was ... | 2011 | 21813776 |
| identification of bacteriocin genes in enterococci isolated from game animals and saltwater fish. | bacteriocins produced by enterococci, referred to as enterocins, possess great interest for their potential use as biopreservatives in food and feed, as well as alternative antimicrobials in humans and animals. in this context, the aim of the present study was to determine the antimicrobial activity and the presence of bacteriocin structural genes in fecal enterococcal isolates from animal origins. evaluation of the direct antimicrobial activity of 253 isolates from wild boars (sus scrofa, n = 6 ... | 2011 | 21819651 |
| a predictive model for the inactivation of listeria innocua in cooked poultry products during postpackage pasteurization. | contamination of listeria monocytogenes in ready-to-eat poultry products poses potential risk of listeriosis to the public. to control the level of listeria contamination, attention has been focused on the postpackage pasteurization of fully cooked poultry products. in this study, we sought to develop a model to predict the thermal inactivation of l. monocytogenes in chicken drumettes during postpackage hot water pasteurization. fully cooked chicken drumettes were inoculated with listeria innocu ... | 2011 | 21819652 |
| quantitative risk assessment of listeriosis due to consumption of raw milk. | the objectives of this study were to estimate the risk of illness for raw milk consumers due to listeria monocytogenes in raw milk sold by permitted dealers, and the risk for people on farms who consume raw milk. three scenarios were evaluated for raw milk sold by dealers: raw milk purchased directly from bulk tanks, from on-farm stores, and from retail. to assess the effect of mandatory testing of raw milk by regulatory agencies, the number of listeriosis cases per year was compared where no ra ... | 2011 | 21819653 |
| reduced detectability of listeria monocytogenes in the presence of listeria innocua. | recent foodborne crises have demonstrated the importance of monitoring food safety. in terms of microbiological criteria, food safety requires the reliable detection of pathogens such as listeria monocytogenes along the food chain by appropriate analytical methods. however, indications exist that accompanying listeria innocua strains suppress the growth of l. monocytogenes during selective enrichment, which may cause reduced or even inhibited detection. to study these effects, the limit of detec ... | 2011 | 21819654 |
| prevalence of foodborne pathogens in grilled chicken from street vendors and retail outlets in reynosa, tamaulipas, mexico. | we analyzed a total of 70 grilled chicken samples bought randomly from street vendors and retail outlets in the city of reynosa, mexico, to determine the prevalence of escherichia coli (shiga toxin producing and enterotoxin producing), salmonella spp., staphylococcus aureus, listeria spp., and campylobacter spp. using microbiological methods and pcr detection of bacterial sequences. of the 70 samples, 27 (38.5%) were from retail outlets and 43 (61.4%) from street vendors. all specimens were nega ... | 2011 | 21819659 |
| inactivation of trea, the gene encoding a phosphotrehalase, causes increased thermal and osmotic stress resistance in listeria monocytogenes 568 when grown in the presence of trehalose. | the foodborne pathogen, listeria monocytogenes is a problem for food processors and consumers alike, as the organism is resistant to harsh environmental conditions and inimical barriers implemented to prevent the survival and/or growth of harmful bacteria. one mechanism by which listeriae mediates survival is through the accumulation of compatible solutes, such as proline, betaine and carnitine. in other bacteria including escherichia coli, the synthesis and accumulation of another compatible so ... | 2011 | 21821737 |
| streptolysin s-like virulence factors: the continuing saga. | streptolysin s (sls) is a potent cytolytic toxin and virulence factor that is produced by nearly all streptococcus pyogenes strains. despite a 100-year history of research on this toxin, it has only recently been established that sls is just one of an extended family of post-translationally modified virulence factors (the sls-like peptides) that are produced by some streptococci and other gram-positive pathogens, such as listeria monocytogenes and clostridium botulinum. in this review, we descri ... | 2011 | 21822292 |
| variability in biofilm production by listeria monocytogenes correlated to strain origin and growth conditions. | this study aimed to identify factors that influence the development of biofilm by listeria monocytogenes strains and to determine the extent to which biofilm production protects against quaternary ammonium compound (qac) disinfectant challenge. a total of 95 l. monocytogenes strains were studied and biofilm production was assessed as a function of incubation temperature, media ph, strain origin, serotype, and environmental persistence status. attachment and biofilm development (inferred by the l ... | 2011 | 21824672 |
| a protein thermometer controls temperature-dependent transcription of flagellar motility genes in listeria monocytogenes. | facultative bacterial pathogens must adapt to multiple stimuli to persist in the environment or establish infection within a host. temperature is often utilized as a signal to control expression of virulence genes necessary for infection or genes required for persistence in the environment. however, very little is known about the molecular mechanisms that allow bacteria to adapt and respond to temperature fluctuations. listeria monocytogenes (lm) is a food-borne, facultative intracellular pathog ... | 2011 | 21829361 |
| recruitment of the major vault protein by inlk: a listeria monocytogenes strategy to avoid autophagy. | l. monocytogenes is a facultative intracellular bacterium responsible for listeriosis. it is able to invade, survive and replicate in phagocytic and non-phagocytic cells. the infectious process at the cellular level has been extensively studied and many virulence factors have been identified. yet, the role of inlk, a member of the internalin family specific to l. monocytogenes, remains unknown. here, we first show using deletion analysis and in vivo infection, that inlk is a bona fide virulence ... | 2011 | 21829365 |
| signal 3 requirement for memory cd8(+) t-cell activation is determined by the infectious pathogen. | the relevance of direct inflammatory signals (signal 3) for the activation of memory cd8(+) t cells during recall responses is so far unknown. we therefore investigated the direct impact of il-12 and type i ifn on the formation, recall potential and protective capacity of memory t cells. using cd8(+) t cells deficient for il-12 or type i ifn receptors in an adoptive transfer system, we generated memory populations after infection with vaccinia virus, lymphocytic choriomeningitis virus or listeri ... | 2011 | 21830209 |
| evaluation of beneficial attributes for phytate-degrading pediococcus pentosaceus cfr r123. | in this study we have screened and selected potent phytate-degrading lactic acid bacteria (lab), and evaluated their beneficial attributes. around 60 lab strains were isolated from several cereal- and pulse-based conventional fermented preparations. they were screened for their ability to degrade myo-inositol hexakisphosphate (ip6) by cobalt chloride qualitative staining method (plate assay). one of the cultures, pediococcus pentosaceus cfr r123, was capable of degrading both calcium and sodium ... | 2010 | 21831762 |
| characterization of two safe enterococcus strains producing enterocins isolated from egyptian dairy products. | five bacterial cocci isolates were selected from a wide pool of 503 isolates collected from traditional egyptian dairy products on the basis of their inhibitory activities against lactobacillus brevis f145, lactobacillus bulgaricus 340, enterococcus faecium hklhs, listeria ivanovii atcc, listeria innocua cip 80.11 and listeria monocytogenes egde 107776. these 5 isolates were identified as e. faecium tx1330 and e. faecium e980 by 16s rdna amplification and sequencing. the antibacterial activity o ... | 2011 | 21831786 |
| [unusual location of a brain abscess due to listeria monocytogenes.] | here we report a case of sustentorial brain abscess due to listeria monocytogenes. blood culture and procalcitonine blood measurement were negative. l. monocytogenes was isolated from csf after inoculation in castañeda medium. | 2011 | 21835558 |
| lmo0036, an ornithine and putrescine carbamoyltransferase in listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance. | listeria monocytogenes is a foodborne pathogen causing listeriosis. acid is one of the most frequently stresses that foodborne pathogens encounter. the ability to survive and proliferate in acidic environments is a prerequisite for its infection. however, there is limited knowledge about the molecular basis of adaption of l. monocytogenes to acid. arginine deiminase (adi) and agmatine deiminase (agdi) systems are implicated in bacterial tolerance to acidic environments. homologues of adi and agd ... | 2011 | 21835877 |
| a novel c-terminal mutation resulting in constitutive activation of the listeria monocytogenes central virulence regulatory factor prfa. | the environmental bacterium listeria monocytogenes survives and replicates in a variety of diverse ecological niches that range from the soil to the cytosol of infected mammalian cells. the ability of l. monocytogenes to replicate within an infected host requires the expression of a number of secreted bacterial gene products whose expression is regulated by the transcriptional activator prfa. prfa becomes activated following bacterial entry into host cells, however the mechanism by which this ac ... | 2011 | 21835879 |
| antimicrobial susceptibilities of listeria monocytogenes isolated in japan. | the antimicrobial susceptibility of 201 listeria monocytogenes isolates from foods, environments, animals and human patients in japan was determined. all isolates were susceptible to ampicillin, the first choice of drug for listeriosis treatment, chloramphenicol, dihydrostreptomycin, erythromycin, enrofloxacin, gentamicin, kanamycin, lincomycin, nosiheptide, salinomycin, vancomycin, and virginiamycin. a human strain was resistant to oxytetracycline. the minimum inhibitory concentration (mic) for ... | 2011 | 21836384 |
| the lmo0501 gene coding for a putative transcription activator protein in listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions. | in listeria monocytogenes egde, the lmo0501 gene locus encodes a protein similar to the mannitol transcription regulator (mltr) protein in bacillus subtilis and bacillus stearothermophilus. in this study we investigated its functional role in l.-ámonocytogenes egde cells in view of growth under different stress conditions. increased lmo0501 gene expression at mrna level was detected in response to cold, osmotic and organic acid stress exposure. an egde +ölmo0501 mutant strain was diminished in g ... | 2011 | 21839374 |
| prevalence and biofilm-forming ability of listeria monocytogenes in new zealand mussel (perna canaliculus) processing plants. | greenshellôäó mussels are new zealand's largest seafood export species. some export markets require compliance with 'zero' tolerance legislation for listeria monocytogenes in 25-ág of product. even though individually quick frozen (iqf) mussel products are labeled 'to be cooked', and are not classified as ready-to-eat, some markets still require them to comply with the strict policy. three mussel processing plants were assessed for the pattern of l.-ámonocytogenes contamination on raw material, ... | 2011 | 21839390 |
| efficacy of freezing, frozen storage and edible antimicrobial coatings used in combination for control of listeria monocytogenes on roasted turkey stored at chiller temperatures. | the presence and growth of listeria monocytogenes on ready-to-eat (rte) turkey is an important food safety issue. the antilisterial efficacy of four polysaccharide-based edible coatings (starch, chitosan, alginate and pectin) incorporating sodium lactate (sl) and sodium diacetate (sd) as well as commercial preparations opti.form pd4, novagardôäó cb1, protect-m and guardianôäó nr100 were compared against l.┬ámonocytogenes on roasted turkey. pectin coating treatments incorporating sl/sd, opti.form ... | 2011 | 21839391 |
| influence of synthetic peptide inducers on antibacterial activity of enterococci. | antibacterial activity of probiotic enterococci is often related to the production of bacteriocins. there is a variety of enterococcus faecium strains which synthesise one or several enterocins including a, b, p, 96, l50ab and others. in this work we have analysed the probiotic strain e. faecium l3 whose genome contained the genes encoding enterocins ðé, ðæ and a three-component regulatory system. all these genes were found to be expressed in enterococcal strain l3. antimicrobial activity of the ... | 2011 | 21840807 |
| oata, a peptidoglycan o-acetyltransferase involved in listeria monocytogenes immune escape, is critical for virulence. | microbial pathogens have evolved mechanisms to overcome immune responses and successfully infect their host. here, we studied how listeria monocytogenes evades immune detection by peptidoglycan (pgn) modification. by analyzing l. monocytogenes muropeptides, we detected o-acetylated muramic acid residues. we identified an o-acetyltransferase gene, oata, in the l. monocytogenes genome sequence. comparison of pgn from parental and isogenic oata mutant strains showed that the o-acetyltransferase oat ... | 2011 | 21844299 |
| Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes. | Listeria monocytogenes is a Gram-positive intracellular bacterial pathogen that colonizes the cytosol of eukaryotic cells. Recent transcriptomic studies have revealed that intracellular L. monocytogenes alter expression of genes encoding envelope components. However, no comparative global analysis of this cell wall remodeling process is yet known at the protein level. Here, we used high resolution mass spectrometry to define the cell wall proteome of L. monocytogenes growing inside epithelial ce ... | 2011 | 21846725 |
| Antibacterial activity of different degree of hydrolysis of palm kernel expeller peptides against spore-forming and non-spore-forming bacteria. | The goal of this study was to determine inhibitory effect of palm kernel expeller (PKE) peptides of different degree of hydrolysis (DH %) against spore-forming bacteria Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus stearothermophillus, Bacillus subtilis, Bacillus thuringiensis, Clostridium perfringens; and non-spore-forming bacteria Escherichia coli, Lisinibacillus sphaericus, Listeria monocytogenes, Pseudomonas a ... | 2011 | 21848644 |
| caveolin-1 orchestrates tcr synaptic polarity, signal specificity, and function in cd8 t cells. | tcr engagement triggers the polarized recruitment of membrane, actin, and transducer assemblies within the t cell-apc contact that amplify and specify signaling cascades and t effector activity. we report that caveolin-1, a scaffold that regulates polarity and signaling in nonlymphoid cells, is required for optimal tcr-induced actin polymerization, synaptic membrane raft polarity, and function in cd8, but not cd4, t cells. in cd8(+) t cells, caveolin-1 ablation selectively impaired tcr-induced n ... | 2011 | 21849673 |
| Contemporary unconventional clinical use of co-trimoxazole. | Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ... | 2012 | 21851485 |
| prevalence of listeria species in camel sausages from retail markets in aydin province in turkey and rapd analysis of listeria monocytogenes isolates. | samples were taken from 100 camel sausages from the different retail markets in aydin province in the south-west of turkey and they were tested for the presence of listeria spp by biochemical methods. samples were enriched using listeria enrichment broth and they were inoculated onto listeria selective agar. listeria monocytogenes was isolated from nine samples (9%), listeria innocua from 14 samples (14%) and listeria welshimeri from two samples(2%). a 701 bp fragment of listeriolysin o sequence ... | 2006 | 21851683 |
| rnai screen reveals host cell kinases specifically involved in listeria monocytogenes spread from cell to cell. | intracellular bacterial pathogens, such as listeria monocytogenes and rickettsia conorii display actin-based motility in the cytosol of infected cells and spread from cell to cell through the formation of membrane protrusions at the cell cortex. whereas the mechanisms supporting cytosolic actin-based motility are fairly well understood, it is unclear whether specific host factors may be required for supporting the formation and resolution of membrane protrusions. to address this gap in knowledge ... | 2011 | 21853127 |
| recombination and positive selection contributed to the evolution of listeria monocytogenes lineages iii and iv, two distinct and well supported uncommon l. monocytogenes lineages. | listeriamonocytogenes lineages iii and iv represent two uncommon lineages of the human and animal pathogen l. monocytogenes, characterized by occurrence of unusual phenotypic and genetic characteristics that differentiate them from the common lineages i and ii. to gain further insights into the evolution of lineages iii and iv, we amplified and sequenced housekeeping genes (i.e., gap, prs, purm, ribc, and sigb), internalin genes (i.e., inla, inlb, inlc, inlg, inlc2, inld, inle, inlf, and inlh) a ... | 2011 | 21854875 |
| survival of listeria monocytogenes introduced as a post-aging contaminant during storage of low-salt cheddar cheese at 4, 10, and 21°c. | traditional aged cheddar cheese does not support listeria monocytogenes growth and, in fact, gradual inactivation of the organism occurs during storage due to intrinsic characteristics of cheddar cheese, such as presence of starter cultures, salt content, and acidity. however, consuming high-salt (sodium) levels is a health concern and the dairy industry is responding by creating reduced-salt cheeses. the microbiological stability of low-salt cheese has not been well documented. this study exami ... | 2011 | 21854905 |
| probing the role of protein surface charge in the activation of prfa, the central regulator of listeria monocytogenes pathogenesis. | listeria monocytogenes is a food-borne intracellular bacterial pathogen capable of causing serious human disease. l. monocytogenes survival within mammalian cells depends upon the synthesis of a number of secreted virulence factors whose expression is regulated by the transcriptional activator prfa. prfa becomes activated following bacterial entry into host cells where it induces the expression of gene products required for bacterial spread to adjacent cells. activation of prfa appears to occur ... | 2011 | 21858145 |
| measuring bacterial load and immune responses in mice infected with listeria monocytogenes. | listeria monocytogenes (listeria) is a gram-positive facultative intracellular pathogen. mouse studies typically employ intravenous injection of listeria, which results in systemic infection. after injection, listeria quickly disseminates to the spleen and liver due to uptake by cd8α+ dendritic cells and kupffer cells. once phagocytosed, various bacterial proteins enable listeria to escape the phagosome, survive within the cytosol, and infect neighboring cells. during the first three days of inf ... | 2011 | 21860372 |
| subtyping of listeria monocytogenes at the haplotype level by fourier transform infrared (ft-ir) spectroscopy and multivariate statistical analysis. | listeria monocytogenes is a widespread foodborne pathogen that represents a major concern with respect to food safety. rapid identification of this bacterium at a subspecies level is important to trace back an outbreak and improve risk-based inspection programs. a method for subtyping l. monocytogenes at the serotype and haplotype levels was developed using fourier transform infrared (ft-ir) reflectance microscopy. thirty strains of l. monocytogenes belonging to four different pcr serotypes (1/2 ... | 2011 | 21864929 |
| [evaluation of the polymerase chain reaction (pcr) for the detection and identification of listeria monocytogens in fresh cheese coming from the metropolitan area of san josé, costa rica]. | food borne diseases are very important worldwide and their frequency is still high despite the different efforts focused in diminishing their morbidity and mortality. listeria monocytogenes is one of the agents associated in this kind of diseases. in the lactic industry, this bacteria is important since raw milks as well as dairy products have been associated in outbreaks, being fresh cheese one of the most vulnerable products to the contamination with this bacteria. the traditional identificati ... | 2010 | 21866690 |
| cd8α(+) dendritic cells are an obligate cellular entry point for productive infection by listeria monocytogenes. | cd8α(+) dendritic cells (dcs) prime cytotoxic t lymphocytes during viral infections and produce interleukin-12 in response to pathogens. although the loss of cd8α(+) dcs in batf3(-/-) mice increases their susceptibility to several pathogens, we observed that batf3(-/-) mice exhibited enhanced resistance to the intracellular bacterium listeria monocytogenes. in wild-type mice, listeria organisms, initially located in the splenic marginal zone, migrated to the periarteriolar lymphoid sheath (pals) ... | 2011 | 21867927 |
| a fusion protein of flagellin and ovalbumin suppresses the th2 response and prevents murine intestinal allergy. | the toll-like receptor (tlr) 5 agonist flagellin is associated with immunomodulatory functions. | 2011 | 21872305 |
| statins inhibit inos-mediated microbicidal potential of activated monocyte-derived dendritic cells by an ifn-β-dependent mechanism. | statins are prescribed to 25 million people worldwide for treating hypercholesterolemia and reducing the risk of cardiovascular diseases. however, the side effects of statins on immunity, and particularly on dc immunobiology, have not been analyzed in-depth. here, we have investigated the impact of lovastatin treatment during monocyte differentiation into dcs on the responsiveness of the resulting monocyte-derived dcs (modcs) to tlr-mediated activation. lovastatin positively regulated tlr4 signa ... | 2011 | 21874649 |
| listeria meningitis and ventriculitis in an immunocompetent child: case report and literature review. | listeria monocytogenes meningitis is very rare in immunocompetent children. we present a case of a previously healthy 6-year-old girl who developed l. monocytogenes meningitis and ventriculitis. we also review the medical literature on non-neonatal l. monocytogenes meningitis in immunocompetent children. | 2011 | 21877182 |
| antibacterial activity of three medicinal thai plants against campylobacter jejuni and other foodborne pathogens. | leaves of adenanthera pavonina, moringa oleifera and annona squamosa are used in traditional thai medicine to treat dysentery and other diseases. this study investigated the antibacterial activity of these plants against six species of foodborne pathogen. methods and solvents employed to extract active constituents were optimised using the disc diffusion assay. phytochemical analysis of the optimised extracts was performed by thin layer chromatography (tlc). minimum inhibitory concentrations (mi ... | 2011 | 21878033 |
| Diverse metabolic profiles of a Streptomyces strain isolated from a hyper-arid environment. | The metabolic profile of Streptomyces sp. strain C34, isolated from the Chilean hyper-arid Atacama Desert soil, is dependent on the culture media used for its growth. The application of an OSMAC approach on this strain using a range of cultivation media resulted in the isolation and identification of three new compounds from the rare class of 22-membered macrolactone polyketides, named chaxalactins A-C (1-3). In addition, the known compounds deferroxamine E (4), hygromycin A (5), and 5?-dihydroh ... | 2011 | 21879726 |
| defining treatment conditions for pulsed electric field pasteurization of apple juice. | the influence of temperature and the presence of n(α)-lauroyl ethylester (ethyl lauroyl arginate, lae) on the inactivation caused by continuous pulsed electric field treatments (pef) in escherichia coli o157:h7 suspended in apple juice have been investigated to define treatment conditions applicable at industrial scale that promote an equivalent safety level when compared with thermal processing. in the range of experimental conditions investigated (outlet temperature: 20-40 °c, electric field s ... | 2011 | 21880388 |
| Intracellular bacteria and adverse pregnancy outcomes. | This review considers the role of intracellular bacteria in adverse pregnancy outcomes, such as miscarriage, stillbirths, and preterm labour. The cause of miscarriage, stillbirth and preterm labour often remains unexplained. Intracellular bacteria that grow either poorly or not at all on media used routinely to detect human pathogens could be the aetiological agents of these obstetric conditions. For example, Listeria monocytogenes and Coxiella burnetti are intracellular bacteria that have a pre ... | 2011 | 21884294 |
| associations among pathogenic bacteria, parasites, and environmental and land use factors in multiple mixed-use watersheds. | over a five year period (2004-08), 1171 surface water samples were collected from up to 24 sampling locations representing a wide range of stream orders, in a river basin in eastern ontario, canada. water was analyzed for cryptosporidium oocysts and giardia cyst densities, the presence of salmonella enterica subspecies enterica, campylobacter spp., listeria monocytogenes, and escherichia coli o157:h7. the study objective was to explore associations among pathogen densities/occurrence and objecti ... | 2011 | 21889781 |
| rapid, transient, and proportional activation of σ(b) in response to osmotic stress in listeria monocytogenes. | the osmotic activation of sigma b (σ(b)) in listeria monocytogenes was studied by monitoring expression of four known σ(b)-dependent genes, opuca, lmo2230, lmo2085, and sigb. activation was found to be rapid, transient, and proportional to the magnitude of the osmotic stress applied, features that underpin the adaptability of this pathogen. | 2011 | 21890665 |
| successful management of listeria monocytogenes pericarditis: case report and review of the literature. | listeria monocytogenes, although an uncommon cause of illness in the general population, is feared principally because of the morbidity and mortality associated with cns infections. cardiovascular involvement with l. monocytogenes is very rare, and has been limited to endocarditis. we describe a case of listeria pericarditis, which occurred in a 60-year-old man with child-pugh b cirrhosis who presented to the emergency department with asthenia, anorexia, and respiratory distress. the echocardiog ... | 2011 | 21894816 |
| microbiological contamination of digested products from anaerobic co-digestion of bovine manure and agricultural by-products. | this study was performed to investigate the microbiological contamination of digestate product (dp) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. | 2011 | 21899580 |
| a novel poisson distribution-based approach for testing boundaries of real-time pcr assays for food pathogen quantification. | the validation of quantitative real-time pcr systems and above all, proof of the detection limit of this method, is a frequently and intensively discussed topic in food pathogen detection. among proper sample collection, assay design, careful experimental design, execution of real-time pcr, and data analysis, the validation of the method per se ensuring reliable quantification data is of prime importance. the purpose of this study was to evaluate a novel validation tool for real-time pcr assays, ... | 2011 | 21902908 |
| Prevalence and antimicrobial susceptibility of major foodborne pathogens in imported seafood. | Seafood is a leading commodity implicated in foodborne disease outbreaks in the United States. Seafood importation rose dramatically in the past 3 decades and now contributes to more than 80% of the total U.S. seafood supply. However, limited data are available on the microbiological safety of imported seafood. In this study, we obtained a total of 171 salmon, shrimp, and tilapia samples imported from 12 countries in three retail stores in Baton Rouge, LA. The total microbial population and the ... | 2011 | 21902913 |
| inhibition of growth of pathogenic bacteria in raw milk by legume protein esters. | protein isolates from soybean and chickpea, as well as their methylated esters, were tested for their inhibitory action against the propagation of pathogenic bacteria in raw milk during its storage either at room temperature or under refrigeration. raw milk was inoculated with a mixed culture of listeria monocytogenes scott a and salmonella enterica serovar enteritidis strain pt4 at ca. 2 log cfu ml⁻¹. aerobic plate count, coliform count, and presumptive e. coli in raw milk treated with esterifi ... | 2011 | 21902916 |
| comparison of two possible routes of pathogen contamination of spinach leaves in a hydroponic cultivation system. | the route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. three major bacterial pathogens, escherichia coli o157:h7, salmonella, and listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10⁶ and 10³ cfu/ml. in parallel, the pathogens were inoculated onto the growing leaf surface by pipetting, to give concentrations of 10⁶ and 10³ cfu per leaf ... | 2011 | 21902924 |
| a survey of iceberg lettuce for the presence of salmonella, escherichia coli o157:h7, and listeria monocytogenes in japan. | no information has been available on the prevalence of pathogens in fresh produce in japan. in the present study, information was collected on the occurrence of contamination by salmonella, escherichia coli o157:h7, and listeria monocytogenes in iceberg lettuce in a japanese retail store. a total of 419 samples of lettuce that had been harvested in different districts and/or by different producers from july 2008 to march 2009 were examined. a multiplex pcr method was used to simultaneously ident ... | 2011 | 21902925 |
| Reduction of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes with electrolyzed oxidizing water on inoculated hass avocados (Persea americana var. Hass). | This study was intended to evaluate the bactericidal effect of electrolyzed oxidizing water (EOW) and chlorinated water on populations of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes inoculated on avocados (Persea americana var. Hass). In the first experiment, inoculated avocados were treated with a water wash applied by spraying tap water containing 1 mg/liter free chlorine for 15 s (WW); WW treatment and then spraying sodium hypochlorite in water containing 75 mg/l ... | 2011 | 21902927 |
| Food safety objectives for Listeria monocytogenes in Spanish food sampled in cafeterias and restaurants. | To gain more insight into the context of food safety management by public administrations, food safety objectives must be studied. The Valencian administration quantified the prevalence of Listeria monocytogenes in cafeterias and restaurants in this region of Spain between 2002 and 2010. The results obtained from this survey are presented here for 2,262 samples of fish, salad, egg, cold meat, and mayonnaise dishes. Microbiological criteria defined for L. monocytogenes were used to differentiate ... | 2011 | 21902930 |
| protection activity of a novel probiotic strain of bacillus subtilis against salmonella enteritidis infection. | the activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. in observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including salmonella enteritidis, salmonella typhimurium, escherichia co ... | 2011 | 21907374 |
| Posttranslocation chaperone PrsA2 regulates the maturation and secretion of Listeria monocytogenes proprotein virulence factors. | PrsA2 is a conserved posttranslocation chaperone and a peptidyl prolyl cis-trans isomerase (PPIase) that contributes to the virulence of the Gram-positive intracellular pathogen Listeria monocytogenes. One of the phenotypes associated with a prsA2 mutant is decreased activity of the broad-range phospholipase C (PC-PLC). PC-PLC is made as a proenzyme whose maturation is mediated by a metalloprotease (Mpl). The proforms of PC-PLC and Mpl accumulate at the membrane-cell wall interface until a decre ... | 2011 | 21908675 |
| a small molecule deubiquitinase inhibitor increases localization of inducible nitric oxide synthase to the macrophage phagosome and enhances bacterial killing. | macrophages are key mediators of antimicrobial defense and innate immunity. innate intracellular defense mechanisms can be rapidly regulated at the posttranslational level by the coordinated addition and removal of ubiquitin by ubiquitin ligases and deubiquitinases (dubs). while ubiquitin ligases have been extensively studied, the contribution of dubs to macrophage innate immune function is incompletely defined. we therefore employed a small molecule dub inhibitor, wp1130, to probe the role of d ... | 2011 | 21911458 |
| the effect of using citric or acetic acid on survival of listeria monocytogenes during fish protein recovery by isoelectric solubilization and precipitation process. | isoelectric solubilization and precipitation (isp) is a protein recovery process effective at reducing listeria innocua, a nonpathogenic bacterium typically used as a surrogate for l. monocytogenes in recovered trout protein. the response of l. monocytogenes to isp processing was determined and compared to the response of l. innocua. headed and gutted rainbow trout were inoculated with l. monocytogenes (10.16 log cfu/g), homogenized, and ph-adjusted with granular citric acid (ph 2.0 and 2.5) or ... | 2011 | 21913922 |
| [quantitative risk assessment of listeria monocytogenes in bulk cooked meat products]. | to conduct a quantitative risk assessment for listeria monocytogenes in bulk cooked meat products in china. | 2011 | 21914338 |
| diagnosis of listeria monocytogenes meningoencephalitis by real-time pcr for the hly gene. | listeria monocytogenes is a bacterial pathogen that can invade the central nervous system (cns), causing meningoencephalitis and brain abscesses. the diagnosis of cns listeriosis, based on the isolation of the bacteria in the cerebrospinal fluid (csf), can be difficult because of previous antibiotic treatment and a low number of bacteria in the csf. to improve the sensitivity of microbiological diagnosis, we have developed a real-time pcr assay for detecting and quantifying l. monocytogenes dna ... | 2011 | 21918022 |