Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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evaluation of the presence of enterococcus faecalis in root cementum: a confocal laser scanning microscope analysis. | the aim of this study is to address the cause of persistent infection of root cementum by enterococcus faecalis. | 2014 | 24778505 |
health evaluation and survey of zoonotic pathogens in free-ranging capybaras (hydrochoerus hydrochaeris). | capybaras (hydrochoerus hydrochaeris) are the world's largest rodents and play an epidemiologic role in the transmission of zoonotic pathogens, including the causative agents of brazilian spotted fever, leptospirosis, and others. we surveyed the health of 31 free-ranging capybaras at the alberto löfgren state park, são paulo, brazil using a variety of diagnostic methods. hematology and serum chemistry were consistent with mild malnutrition and parasitism but did not indicate severe physiologic i ... | 2014 | 24779462 |
correlation between biofilm formation and gele, esp, and agg genes in enterococcus spp. clinical isolates. | 2014 | 24782231 | |
study comparing the effectiveness of chlorhexidine, calcium hydroxide and linezolid based medicaments against enterococcus faecalis. | this study evaluated the efficacy of 2% chlorhexidine (cx), calcium hydroxide (ch), vitapex® (vp), linezolid (lz), a combination of lz with ch (lc) against enterococcus faecalis (ef). | 2014 | 24783147 |
poultry farm hygiene: microbiological quality assessment of drinking water used in layer chickens managed under the battery cage and deep litter systems at three poultry farms in southwestern nigeria. | water troughs from deep litter and caged chicken water troughs (drinkers) fixed to each of the different 3-tier cages containing layer chickens in farms a, b and c were subjected to a 7-day study which involved the monitoring of poultry farm hygiene. drinkers were washed before filling with water on day 1. for days 3, 5 and 7 water was served without prior washing. the occurrence and characterization of the bacteria isolates were investigated and data obtained were analyzed and compared. for the ... | 2014 | 24783781 |
diverse outcomes of photodynamic antimicrobial chemotherapy on five enterococcus faecalis strains. | in the present study, the effectiveness of photodynamic antimicrobial chemotherapy (pact) was evaluated on planktonic cells and biofilms of five enterococcus faecalis clinical isolates. | 2014 | 24784432 |
essential oil composition and antimicrobial activity of angelica archangelica l. (apiaceae) roots. | in this paper, the chemical composition and the antimicrobial activity of the essential oil of angelica archangelica l. (apiaceae) roots from central italy were analyzed. the major constituents of the oil were α-pinene (21.3%), δ-3-carene (16.5%), limonene (16.4%) and α-phellandrene (8.7%). the oil shows a good antimicrobial activity against clostridium difficile, clostridium perfringens, enterococcus faecalis, eubacterium limosum, peptostreptococcus anaerobius, and candida albicans with minimum ... | 2014 | 24788027 |
bacterial leakage in root canals filled with ah plus and dentine bonding agents. | the aim of this study was to compare the efficacy of different dentine adhesives in delaying the coronal bacterial leakage of enterococcus faecalis in filled root canals. materials and methods. ninety-five lower incisors of patients >65 years of age were instrumented using the protaper system and were irrigated with 1 ml of 2.5% sodium hypochlorite (naocl) alternated with 1 ml 17% edta between each file change. final irrigation was performed with 5 ml of 17% edta and then flushed with 5 ml of di ... | 2014 | 24791609 |
antibacterial activity of kaolinite/nanotio2 composites in relation to irradiation time. | the paper addresses laboratory preparation and antibacterial activity testing of kaolinite/nanotio2 composite in respect of the daylight irradiation time. kaolinite/nanotio2 composites with 20 and 40 wt% of tio2 were laboratory prepared, dried at 105 °c and calcined at 600 °c. the calcination caused transformation of kaolinite to metakaolinite and origination of the metakaolinite/nanotio2 composite. x-ray powder diffraction, raman and ftir spectroscopic methods revealed titanium dioxide only in ... | 2014 | 24792569 |
enterococcus faecalis bacteremia and mitral valve endocarditis under dabigatran for stroke prevention. | 2014 | 24794060 | |
in vitro evaluation of antimicrobial features of vasopressors. | drugs administered as intravenous infusion may be contaminated during several stages of production or preparation. however studies focusing on antibacterial effects of vasopressor drugs are very rare. this study investigates the in vitro antimicrobial activity of the clinically used forms of vasopressors. | 2014 | 24794449 |
occurrence of urinary tract infection in adolescent and adult women of shanty town in dhaka city, bangladesh. | urinary tract infection (uti) is commonly experienced by women of various age groups especially elderly ones. we planned to find out the prevalent microbial strains causing uti in slum inhabitant adolescent and adult women in dhaka city, bangladesh. | 2014 | 24795516 |
impact of different antimicrobial therapies on clinical and fiscal outcomes of patients with bacteremia due to vancomycin-resistant enterococci. | vancomycin-resistant enterococci (vre) are a growing health problem, and uncertainties exist regarding the optimal therapy for bloodstream infection due to vre. we conducted systematic comparative evaluations of the impact of different antimicrobial therapies on the outcomes of patients with bloodstream infections due to vre. a retrospective study from january 2008 to october 2010 was conducted at detroit medical center. unique patients with blood cultures due to vre were included and reviewed. ... | 2014 | 24798267 |
surotomycin demonstrates low in vitro frequency of resistance and rapid bactericidal activity in clostridium difficile, enterococcus faecalis, and enterococcus faecium. | surotomycin (cb-183,315) is an orally administered, minimally absorbed, selective bactericidal cyclic lipopeptide in phase 3 development for the treatment of clostridium difficile-associated diarrhea. the aim of this study was to evaluate the emergence of resistance in c. difficile (atcc 700057 and three recent clinical isolates from the restriction endonuclease analysis groups bi, bk, and k), vancomycin-susceptible (vs) enterococcus faecalis (atcc 49452), vancomycin-resistant (vr) e. faecalis ( ... | 2014 | 24798273 |
'honey ointment': a natural remedy of skin wound infections. | honey is a gift of nature, principally identified and valued to possess antimicrobial and anti-inflammatory activity and has been used as a natural remedy of wounds since ancient times. the objectives of this study were to evaluate the antimicrobial activity of honey against micro-organisms, to formulate a honey ointment and to evaluate the efficacy of such ointment by conducting clinical trials on skin wound infection. | 2014 | 24800336 |
colloidal gold-based immunochromatographic strip test compromising optimised combinations of anti-s. suis capsular polysaccharide polyclonal antibodies for detection of streptococcus suis. | a rapid diagnosis kit that detects streptococcus suis (s. suis) antigens from urine with an immunochromatographic stripe (ics) test was developed in this study. the ics test was produced using colloidal gold coated with polyclonal antibodies (pabs) against s. suis. the pabs were developed from rabbits immunised with s. suis serotype 2 capsular polysaccharides (cps). development of the pabs was investigated to establish their binding to cps and to determine the maximum sensitivity of two combinat ... | 2014 | 24800681 |
complete genome sequencing and comparative analysis of the linezolid-resistant enterococcus faecalis strain deng1. | genome level analysis of bacterial strains provides information on genetic composition and resistance mechanisms to clinically relevant antibiotics. to date, whole genome characterization of linezolid-resistant enterococcus faecalis isolated in the clinic is lacking. in this study, we report the entire genome sequence, genomic characteristics and virulence factors of a pathogenic e. faecalis strain, deng1. our results showed considerable differences in genomic characteristics and virulence facto ... | 2014 | 24800692 |
fabrication and characterization of bioactive and antibacterial composites for dental applications. | there is an increasing clinical need to design novel dental materials that combine regenerative and antibacterial properties. in this work the characterization of a recently developed sol-gel-derived bioactive glass ceramic containing silver ions (ag-bg) is presented. the microstructural characteristics, ion release profile, zeta potential value and changes in weight loss and ph value as a function of the immersion time of ag-bg in tris buffer are evaluated. ag-bg is also incorporated into natur ... | 2014 | 24802300 |
comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white-coloured mineral trioxide aggregate. | to comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (rdsc) and proroot white-coloured mineral trioxide aggregate (wmta). | 2015 | 24802368 |
paenibacterin, a novel broad-spectrum lipopeptide antibiotic, neutralises endotoxins and promotes survival in a murine model of pseudomonas aeruginosa-induced sepsis. | paenibacterin, produced by paenibacillus thiaminolyticus osy-se, is active both against gram-negative and gram-positive pathogens, including antibiotic-resistant strains of escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, klebsiella pneumoniae, staphylococcus aureus and enterococcus faecalis. paenibacterin showed relatively low cytotoxicity against a human kidney cell line (atcc crl-2190), with a 50% inhibitory concentration (ic50)≥109μg/ml. the cationic paenibacterin molecule ... | 2014 | 24802906 |
role of glycolipids in the pathogenesis of enterococcus faecalis urinary tract infection. | after uropathogenic escherichia coli (upec), enterococcus faecalis is the second most common pathogen causing urinary tract infections. monoglucosyl-diacylglycerol (mglcdag) and diglucosyl-diacylglycerol (dglcdag) are the main glycolipids of the e. faecalis cell membrane. examination of two mutants in genes bgsb and bgsa (both glycosyltransferases) showed that these genes are involved in cell membrane glycolipid biosynthesis, and that their inactivation leads to loss of glycolipids dglcdag (bgsa ... | 2014 | 24806450 |
incidence, risk factors, and outcomes for enterococcus spp. blood stream infections: a population-based study. | enterococci are a clinically significant cause of bloodstream infections (bsi), particularly in the nosocomial setting. the purpose of this study was to characterize the incidence, risk factors for acquisition, microbiological characteristics and mortality of enterococcal bsi within the well-defined population of a large canadian health region. | 2014 | 24813873 |
microbial population dynamics in the hemolymph of manduca sexta infected with xenorhabdus nematophila and the entomopathogenic nematode steinernema carpocapsae. | xenorhabdus nematophila engages in a mutualistic association with the nematode steinernema carpocapsae. the nematode invades and traverses the gut of susceptible insects. x. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. x. nematophila produces diverse antimicrobials in laboratory cultures. the natural competitors that x. nematophila encounters in the hemolymph and the role of antimicrobials in interspecies competit ... | 2014 | 24814780 |
investigation of mechanism and molecular epidemiology of linezolid-resistant enterococcus faecalis in china. | enterococcus is a major cause of important nosocomial infections. linezolid, the first member of an entirely new class of antibiotics (oxazolidinones), is effective against serious infections caused by enterococcus. however, resistance to linezolid has been discovered throughout the world rapidly. from 2011 to 2013, nine linezolid-resistant e. faecalis isolates were collected and the possible mechanisms of linezolid resistance, including mutations in domain v of 23s rrna genes and in ribosomal p ... | 2014 | 24815727 |
infliximab therapy increases the frequency of circulating cd16(+) monocytes and modifies macrophage cytokine response to bacterial infection. | crohn's disease (cd) has been correlated with altered macrophage response to microorganisms. considering the efficacy of infliximab treatment on cd remission, we investigated infliximab effects on circulating monocyte subsets and on macrophage cytokine response to bacteria. human peripheral blood monocyte-derived macrophages were obtained from cd patients, treated or not with infliximab. macrophages were infected with escherichia coli, enterococcus faecalis, mycobacterium avium subsp. paratuberc ... | 2014 | 24816497 |
efficacy of three irrigation agitation techniques on bacterial elimination: a microbiologic and microscopic evaluation. | the aim of this study was to compare in vitro reduction of enterococcus faecalis by using three agitation methods. fifty-five mandibular premolar teeth were used. the root canals were prepared by using protaper instruments to size f4. fresh bacterial suspensions were inoculated into each canal and the teeth were incubated at 37°c under aerobic conditions for 3 weeks. the specimens in negative control group were not contaminated and the teeth in positive control receive no further instrumentation ... | 2014 | 24817336 |
genome analysis of enterococcus faecalis bacteriophage ime-ef3 harboring a putative metallo-beta-lactamase gene. | lytic enterococcus faecalis bacteriophage ime-ef3 was isolated from hospital sewage, and its genome was sequenced using high-throughput sequencing. genomic analysis and electron microscopy suggested that ime-ef3 was a member of the family siphoviridae. the phage has an isometric head and a long non-contractile tail with a 41 kb linear double-stranded dna genome. the genome encodes 69 putative proteins, with 32 annotated functionally, including proteins related to phage structure, packaging, tran ... | 2014 | 24818695 |
isolation and biological activity of compounds from garcinia preussii. | plants of the genus garcinia (clusiaceae) are traditionally used to relieve stomachaches, toothaches, and as a chew stick. | 2014 | 24824323 |
iliopsoas abscess in children: report on five patients with a literature review. | we aimed in this study to present the clinical findings in children with iliopsoas abscess (ipa) and to discuss the diagnosis and treatment. the files of five patients, hospitalized between august 2011 and june 2013 and monitored with a diagnosis of ipa, were reviewed retrospectively. demographic characteristics, symptoms and signs, laboratory examinations, and diagnostic and treatment methods of the cases were evaluated. two of the cases were females and three were males, and their ages ranged ... | 2014 | 24827950 |
structural and electronic snapshots during the transition from a cu(ii) to cu(i) metal center of a lytic polysaccharide monooxygenase by x-ray photoreduction. | lytic polysaccharide monooxygenases (lpmos) are a recently discovered class of enzymes that employ a copper-mediated, oxidative mechanism to cleave glycosidic bonds. the lpmo catalytic mechanism likely requires that molecular oxygen first binds to cu(i), but the oxidation state in many reported lpmo structures is ambiguous, and the changes in the lpmo active site required to accommodate both oxidation states of copper have not been fully elucidated. here, a diffraction data collection strategy m ... | 2014 | 24828494 |
prevalence and detection of mixed-population enterococcal bacteremia. | mixed-population (heterogeneous) enterococcal bacteremia (meb) is rarely reported. based on one occasion in which vitek2 missed a vancomycin-resistant subpopulation isolated from a patient, we developed a simple method to detect this subpopulation and determined meb frequency. the four patients presented here had either enterococcus faecium or enterococcus faecalis bacteremia caused by both vancomycin-resistant enterococci (vre) and vancomycin-susceptible enterococci (vse). no prior common antib ... | 2014 | 24829233 |
enterococcus faecalis from healthy infants modulates inflammation through mapk signaling pathways. | colonizing commensal bacteria after birth are required for the proper development of the gastrointestinal tract. it is believed that bacterial colonization pattern in neonatal gut affects gut barrier function and immune system maturation. studies on the development of faecal microbiota in infants showed that the neonatal gut was first colonized with enterococci followed by other microbiota such as bifidobacterium. other studies showed that babies who developed allergy were less often colonized w ... | 2014 | 24830946 |
urinary tract infection caused by enterococcus isolates: aetiology and antimicrobial resistance patterns. | the purpose of this study was to analyse the data on resistance in enterococcus faecalis and e. faecium isolated from urine samples of inpatients from january 2010 to december 2013. compared to e. faecalis, e. faecium isolates were significantly more resistant to ampicilin, nitrofurantoin, and ciprofloxacin in the antimicrobial agents evaluated. both species showed higher resistance to high-level gentamicin. the rate of vancomycin-resistant enterococci (vre) in e. faecium was higher as compared ... | 2015 | 24835052 |
synthesis, characterization, antimicrobial activity and carbonic anhydrase enzyme inhibitor effects of salicilaldehyde-n-methyl p-toluenesulfonylhydrazone and its palladium(ii), cobalt(ii) complexes. | we report the synthesis of the ligand, salicilaldehyde-n-methyl p-toluenesulfonylhydrazone (salptsmh) derived from p-toluenesulfonicacid-1-methylhydrazide (ptsmh) and its pd(ii) and co(ii) metal complexes were synthesized for the first time. the structure of the ligand and their complexes were investigated using elemental analysis, magnetic susceptibility, molar conductance and spectral (ir, nmr and lc-ms) measurements. salptsmh has also been characterized by single crystal x-ray diffraction. (1 ... | 2014 | 24835932 |
comparative genomic and morphological analyses of listeria phages isolated from farm environments. | the genus listeria is ubiquitous in the environment and includes the globally important food-borne pathogen listeria monocytogenes. while the genomic diversity of listeria has been well studied, considerably less is known about the genomic and morphological diversity of listeria bacteriophages. in this study, we sequenced and analyzed the genomes of 14 listeria phages isolated mostly from new york dairy farm environments as well as one related enterococcus faecalis phage to obtain information on ... | 2014 | 24837381 |
antibacterial activity of tham trisphenylguanide against methicillin-resistant staphylococcus aureus. | this study investigated the potential antibacterial activity of three series of compounds synthesized from 12 linear and branched polyamines with 2-8 amino groups, which were substituted to produce the corresponding guanides, biguanides, or phenylguanides, against acinetobacter baumannii, enterococcus faecalis, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus. antibacterial activity was measured for each compound by determining the minimum inhibitory concentration against the b ... | 2014 | 24840307 |
formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii. | bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt. | 2015 | 24842546 |
low-dose cisplatin administration to septic mice improves bacterial clearance and programs peritoneal macrophage polarization to m1 phenotype. | sepsis is a systemic inflammatory response to infection, and early responses of macrophages are vital in controlling the infected microorganisms. we used a cecal ligation and puncture (clp) model of sepsis to determine the role of cisplatin (0.1, 0.5 and 1 mg kg(-1)) with respect to peritoneal macrophages, controlling peritoneal/blood bacterial infection, and systemic inflammation. we found that mice which received low-dose (0.1 and 0.5 mg kg(-1)) i.p. cisplatin had lower mortality rate and impr ... | 2014 | 24850793 |
comparative analysis of pathogenic organisms in cockroaches from different community settings in edo state, nigeria. | cockroaches are abundant in nigeria and are seen to harbour an array of pathogens. environmental and sanitary conditions associated with demographic/socio-economic settings of an area could contribute to the prevalence of disease pathogens in cockroaches. a total of 246 cockroaches (periplaneta americana) in urban (benin, n=91), semi-urban (ekpoma, n=75) and rural (emuhi, n=70) settings in edo state, nigeria were collected within and around households. the external body surfaces and alimentary c ... | 2014 | 24850961 |
protein content analysis and antimicrobial activity of the crude venom of montivipera bornmuelleri; a viper from lebanon. | viperidae snakes venoms represent a source of efficient bioactive components that have already led to the development of several new drugs. in this work, we analyzed the protein content of the montivipera bornmuelleri crude venom using lc-esi-ms, sephadex g-75 gel filtration and sds-page and demonstrated the presence of proteins with molecular masses corresponding to metalloprotease iii, serine-protease and pla2 in three fractions collected after gel filtration. equally, we examined the antimicr ... | 2014 | 24853875 |
detection of opsonic antibodies against enterococcus faecalis cell wall carbohydrates in immune globulin preparations. | three different commercially available polyvalent immune globulins (ig) were investigated for the existence of antibodies against cell wall carbohydrates of four different e. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). all three ig preparations contained antibodies against all four serotypes (cps-a to cps-d). however, only one of the three ig preparations showed opso ... | 2014 | 24854332 |
[clinical distribution and antimicrobial resistance analysis of 754 pathogenic bacteria in diabetic foot infection]. | to explore the microbiological profiles and antibiotic susceptibility patterns of organisms isolated from diabetic foot ulcers so as to provide selection rationales of antibiotics. | 2014 | 24854906 |
synthesis and characterization of antimicrobial crosslinked carboxymethyl chitosan nanoparticles loaded with silver. | carboxymethyl chitosan (cmch)-silver nanoparticle (ag) hydrogels with high antibacterial activity against three gram +ve bacteria (staphylococcus aureus, bacillus subtilis and streptococcus faecalis), three gram -ve bacteria (escherichia coli, pseudomonas aeruginosa and neisseria gonorrhoeae) and a candida albicans fungus were prepared. the in situ preparation reaction involved crosslinking of cmch with epichlorohydrin in alkaline medium containing silver nitrate to yield silver nanoparticles lo ... | 2014 | 24857868 |
development of an immunochromatographic test with anti-lipl32-coupled gold nanoparticles for leptospira detection. | detection of antibody specific to leptospira by various immunological techniques has been used for leptospirosis diagnosis. however, the sensitivity of antibody detection during the first few days after infection is low. molecular techniques are suggested to provide earlier diagnosis than antibody detection, but a rapid and easy to perform assay for leptospira antigen detection would provide an additional useful tool for disease diagnosis. in this study, we coupled gold nanoparticles with antibo ... | 2014 | 24858647 |
in vitro and ex vivo antimicrobial efficacy of nano-mgo in the elimination of endodontic pathogens. | the use of metal oxide nanoparticles has attracted lots of attention, mostly because of their promising antimicrobial activity along with their biocompatibility with mammalian cells. this study aims to investigate the in vitro and ex vivo antimicrobial efficiency of nano-magnesium oxide (mgo) aqueous solution against endodontic pathogens. | 2015 | 24859291 |
comparative antibacterial efficacy of photodynamic therapy and ultrasonic irrigation against enterococcus faecalis in vitro. | enterococcus faecalis poses a challenge to the efficacy of traditional root canal disinfection methods. this study was aimed to establish a synergistic root canal disinfection strategy combining ultrasonic irrigation with photodynamic therapy (pdt) together and to test its antibacterial efficacy against e. faecalis. twenty-seven bovine root canals infected with e. faecalis were randomly divided into three groups and treated with different disinfection methods as follows: ultrasonic irrigation wi ... | 2014 | 24861937 |
extension of bactericidal effect of sodium hypochlorite into dentinal tubules. | the aim of this study was to determine the extent of disinfection mediated by 2 concentrations of sodium hypochlorite for bacteria-laden dentin wall of a root canal. | 2014 | 24862710 |
eco-designed biohybrids based on liposomes, mint-nanosilver and carbon nanotubes for antioxidant and antimicrobial coating. | noncovalent entities (consisting of liposomes, phyto-nanosilver and carbon nanotubes) with interesting properties were constructed by a "green" bottom-up method. phytosynthesis of silver nanoparticles using the mentha piperita extract combines the benefits of this herb with the interesting properties of silver. the obtained silver-based biohybrids showed antioxidant and antimicrobial properties that have been considerable improved in the presence of carbon nanotubes. thus the eco-designed biocon ... | 2014 | 24863214 |
demography and burden of care associated with patients readmitted for urinary tract infection. | urinary tract infection (uti) is one of the most prevalent admission diagnoses in hospital-based clinical practice. despite its frequency, few data are available regarding its demographics and economic implications. | 2015 | 24863498 |
activity of daptomycin or linezolid in combination with rifampin or gentamicin against biofilm-forming enterococcus faecalis or e. faecium in an in vitro pharmacodynamic model using simulated endocardial vegetations and an in vivo survival assay using galleria mellonella larvae. | enterococci are the third most frequent cause of infective endocarditis. a high-inoculum stationary-phase in vitro pharmacodynamic model with simulated endocardial vegetations was used to simulate the human pharmacokinetics of daptomycin at 6 or 10 mg/kg of body weight/day or linezolid at 600 mg every 12 h (q12h), alone or in combination with gentamicin at 1.3 mg/kg q12h or rifampin at 300 mg q8h or 900 mg q24h. biofilm-forming, vancomycin-susceptible enterococcus faecalis and vancomycin-resista ... | 2014 | 24867993 |
assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex vivo. | to investigate the dentinal tubule invasion capacity of enterococcus faecalis under alkaline and energy starvation stress conditions. | 2015 | 24872016 |
purulent pericarditis after liver abscess: a case report. | we present the case of a 49-year-old woman, with previous clinical antecedents of recent hepatic metastasis, who was admitted to the icu due to respiratory failure and hemodynamic instability. she was found to have purulent pericarditis complicated by pericardial tamponade and pleural effusion, as well as surgical site infection, which was the origin of the disease. cultures of the surgical wound and the pericardial effusion were positive for enterococcus faecalis and escherichia coli. a pericar ... | 2014 | 24872819 |
evaluation of efficacy of combinations of five endodontic sealers with five antibiotics against enterococcus faecalis - an in-vitro study. | to evaluate and compare in vitro the antibacterial efficacy of five antibiotics when added individually to five endodontic sealers against enterococcus faecalis (ef). | 2014 | 24876708 |
genetic and biochemical evidence that recombinant enterococcus spp. strains expressing gelatinase (gele) produce bovine milk-derived hydrolysates with high angiotensin converting enzyme-inhibitory activity (ace-ia). | in this work, genes encoding gelatinase (gele) and serine proteinase (spre), two extracellular proteases produced by enterococcus faecalis dbh18, were cloned in the protein expression vector pmg36c, containing the constitutive p32 promoter, generating the recombinant plasmids pcg, pcsp, and pcgsp encoding gele, spre, and gele-spre, respectively. transformation of noncaseinolytic e. faecalis p36, e. faecalis jh2-2, e. faecium ar24, and e. hirae ar14 strains with these plasmids permitted detection ... | 2014 | 24877744 |
multispecies biofilm in an artificial wound bed--a novel model for in vitro assessment of solid antimicrobial dressings. | wound infections represent a major problem, particularly in patients with chronic wounds. bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. a simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. the method is based on the well-established lubbock chronic wound biofilm transferred onto an artificial agar woun ... | 2014 | 24880129 |
inhibition of enterococcus faecalis biofilm formation by highly active lactones and lactams analogues of rubrolides. | seven β-aryl substituted γ-alkylidene-γ-lactones analogues of rubrolides were synthesized from mucobromic acid and converted through a lactamization with isobutylamine into their corresponding γ-hydroxy-γ-lactams (76-85%). these lactams were converted into (z)- and (e)-γ-alkylidene-γ-lactams (23-45%). all compounds were fully characterized by ir, nmr ((1)h and (13)c), cosy and hetcor bidimensional experiments, and noe difference spectroscopy experiments when necessary. evaluation of these three ... | 2014 | 24880232 |
linezolid update: stable in vitro activity following more than a decade of clinical use and summary of associated resistance mechanisms. | linezolid, approved for clinical use since 2000, has become an important addition to the anti-gram-positive infection armamentarium. this oxazolidinone drug has in vitro and in vivo activity against essentially all gram-positive organisms, including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococci (vre). the in vitro activity of linezolid was well documented prior to its clinical application, and several ongoing surveillance studies demonstrated consistent ... | 2014 | 24880801 |
characterisation of the antibacterial effect of polyethyleneimine nanoparticles in relation to particle distribution in resin composite. | to characterise the antibacterial effect of resin composite incorporating cross-linked quaternised polyethyleneimine (qpei) nanoparticles in relation to their distribution in the bulk material. | 2015 | 24881908 |
recurring hyperammonemic encephalopathy induced by bacteria usually not producing urease. | hyperammonemic encephalopathy may occur when urease-positive bacteria in the urinary tract produce ammonium which directly enters systemic circulation. predisposing conditions such as a neurogenic bladder can increase both urinary tract infection and urine stagnation. | 2014 | 24884855 |
improving photodynamic inactivation of bacteria in dentistry: highly effective and fast killing of oral key pathogens with novel tooth-colored type-ii photosensitizers. | increasing antibiotic resistances in microorganisms create serious problems in public health. this demands alternative approaches for killing pathogens to supplement standard treatment methods. photodynamic inactivation of bacteria (pib) uses light activated photosensitizers (ps) to generate reactive oxygen species immediately upon illumination, inducing lethal phototoxicity. positively charged phenalen-1-one derivatives are a new generation of ps for light-mediated killing of pathogens with out ... | 2014 | 24884918 |
persistence of microbial communities including pseudomonas aeruginosa in a hospital environment: a potential health hazard. | the persistence of microbial communities and how they change in indoor environments is of immense interest to public health. moreover, hospital acquired infections are significant contributors to morbidity and mortality. evidence suggests that, in hospital environments agent transfer between surfaces causes healthcare associated infections in humans, and that surfaces are an important transmission route and may act as a reservoir for some of the pathogens.this study aimed to evaluate the diversi ... | 2014 | 24885173 |
in vitro synergy of ampicillin with gentamicin, ceftriaxone and ciprofloxacin against enterococcus faecalis. | 2014 | 24888868 | |
australian group on antimicrobial resistance enterococcus surveillance programme annual report, 2010. | in 2010, 15 institutions around australia conducted a period prevalence study of key resistances in isolates of enterococcus species associated with a range of clinical disease amongst in- and outpatients. each institution collected up to 100 consecutive isolates and tested these for susceptibility to commonly used antimicrobials using standardised methods. vancomycin-resistant enterococcus faecium and enterococcus faecalis were characterised by pulsed-field gel electrophoresis. multilocus seque ... | 2013 | 24890955 |
phage neutralization by sera of patients receiving phage therapy. | the aim of our investigation was to verify whether phage therapy (pt) can induce antiphage antibodies. the antiphage activity was determined in sera from 122 patients from the phage therapy unit in wrocław with bacterial infections before and during pt, and in sera from 30 healthy volunteers using a neutralization test. furthermore, levels of antiphage antibodies were investigated in sera of 19 patients receiving staphylococcal phages and sera of 20 healthy volunteers using enzyme-linked immunos ... | 2014 | 24893003 |
production of lactic acid using a new homofermentative enterococcus faecalis isolate. | lactic acid is an intermediate-volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. in this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, fac ... | 2015 | 24894833 |
chemical composition of enterococcus faecalis in biofilm cells initiated from different physiologic states. | enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. however, characteristics of chemical composition of biofilm cells developed by starved e. faecalis cells remain poorly understood. in this study, e. faecalis cells in exponential, stationary, and starvation phases were prepared and separately cultured to fo ... | 2014 | 24895050 |
bactericidal effect of hydroxyl radicals generated from a low concentration hydrogen peroxide with ultrasound in endodontic treatment. | one approach to enhance the disinfection of root canals in endodontic treatment is ultrasonic irrigation with sodium hypochlorite. reactive oxygen species, such as hydroxyl radical, are generated by biological defense systems to kill invading bacteria. ultrasonic irrigation with hydrogen peroxide may be a promising option to increase hydroxyl radical generation. we examined the bactericidal effects of hydroxyl radical generated from low concentration hydrogen peroxide with ultrasound in vitro. a ... | 2014 | 24895478 |
modified lactic acid bacteria detect and inhibit multiresistant enterococci. | we designed lactococcus lactis to detect enterococcus faecalis. upon detection, l. lactis produce and secrete antienterococcal peptides. the peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of l. lactis. the enterococcal sex pheromone ccf10 serves as the signal for detection. expression vectors derived from pcf10, a ccf10-responsive e. faecalis sex-pheromone conjugative plasmid, were engineered in l. lactis for the detection system. recombinant host strain ... | 2015 | 24896372 |
[submucosal bacterial abscesses of the ascending colon and liver associated with portal and superior mesenteric vein thrombosis due to enterococcus faecalis infection: a case report]. | a 72-year-old woman with diabetes mellitus was admitted with fever and general fatigue. blood biochemistry showed elevated hepatic and biliary enzyme levels, abdominal computed tomography showed multiple liver abscesses with portal and superior mesenteric vein thrombosis, and total colonoscopy revealed a submucosal bacterial abscess in the ascending colon. the abscesses were determined to be associated with enterococcus faecalis infection. the patient was treated conservatively with antibiotics ... | 2014 | 24898493 |
potent oxazolidinone antibacterials with heteroaromatic c-ring substructure. | novel oxazolidinone analogues bearing a condensed heteroaromatic ring as the c-ring substructure were synthesized as candidate antibacterial agents. analogues 16 and 21 bearing imidazo[1,2-a]pyridine and 18 and 23 bearing [1,2,4]triazolo[1,5-a]pyridine as the c-ring had excellent in vitro antibacterial activities against methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococcus faecalis (vre), and penicillin-resistant streptococcus pneumoniae (prsp). they also showed ... | 2013 | 24900607 |
commensal bacteria drive endogenous transformation and tumour stem cell marker expression through a bystander effect. | commensal bacteria and innate immunity play a major role in the development of colorectal cancer (crc). we propose that selected commensals polarise colon macrophages to produce endogenous mutagens that initiate chromosomal instability (cin), lead to expression of progenitor and tumour stem cell markers, and drive crc through a bystander effect. | 2015 | 24906974 |
immunomodulatory properties of cell wall extract from enterococcus faecalis cect7121. | the aim of this study was to investigate the immunomodulatory properties of cell wall extract from enterococcus faecalis cect7121, measuring the induction of cytokines tnf-α, il-6, il-10 and il-12 from human peripheral blood mononuclear cells (pbmcs). cell wall extract was prepared from their growth in brain heart infusion broth (18h, 35°c). subsequently, toxicity of the obtained cell wall extract was tested in balb-c mice. pbmcs were isolated from buffy coats at the blood transfusion service of ... | 2014 | 24907474 |
determination of kinetics and the crystal structure of a novel type 2 isopentenyl diphosphate: dimethylallyl diphosphate isomerase from streptococcus pneumoniae. | isopentenyl diphosphate isomerase (idi) is a key enzyme in the isoprenoid biosynthetic pathway and is required for all organisms that synthesize isoprenoid metabolites from mevalonate. type 1 idi (idi-1) is a metalloprotein that is found in eukaryotes, whereas the type 2 isoform (idi-2) is a flavoenzyme found in bacteria that is completely absent from human. idi-2 from the pathogenic bacterium streptococcus pneumoniae was recombinantly expressed in escherichia coli. steady-state kinetic studies ... | 2014 | 24910111 |
biological neutralization and biosorption of dyes of alkaline textile industry wastewater. | the present work was aimed to secure biological neutralization and biosorption of dyes of an alkaline textile industrial effluent (atie) using an alkaliphilic bacterium, enterococcus faecalis strain r-16 isolated from gujarat coast. the isolate was capable and competent to bring down the ph of atie from 12.1 to 7.0 within 2 h in the presence of carbon and nitrogen sources. carboxylic group concentration (cgc), nmr and ft-ir analysis revealed production of carboxylic acid as a result of neutraliz ... | 2014 | 24910184 |
residual antimicrobial activity of mtad(®) in human dentin after obturation with gutta-percha/ah26 and resilon/realseal se at different time intervals; an ex vivo study. | to eliminate microorganisms that are responsible for pulpal and periapical infections and to prevent reinfection of the root canal system an effective chemomechanical preparation by irrigants with sustained antimicrobial activity is beneficial. hereby, we evaluated the residual antibacterial activity of mtad after canal obturation at different time intervals. | 2014 | 24910674 |
involvement of enterococcus faecalis small rnas in stress response and virulence. | candidate small rnas (srnas) have recently been identified in enterococcus faecalis, a gram-positive opportunistic pathogen, and six of these candidate srnas with unknown functions were selected for a functional study. deletion mutants and complemented strains were constructed, and their virulence was tested. we were unable to obtain the ef0869-0870 mutant, likely due to an essential role, and the ef0820-0821 srna seemed not to be involved in virulence. in contrast, the mutant lacking ef0408-040 ... | 2014 | 24914223 |
frequency and antibiotic susceptibility patterns of urinary pathogens in male outpatients in argentina. | knowledge of the etiology and antimicrobial susceptibility patterns of uropathogens is important for determining the best treatment option. this study aimed to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from adult male outpatients. | 2014 | 24916867 |
quality control mic ranges used for telavancin with application of a revised clsi reference broth microdilution method. | the telavancin broth microdilution susceptibility testing method was revised, which provides mic results lower than those obtained by the previous method. this study was performed to reestablish the quality control ranges for telavancin when tested against the strains (updated mic range) staphylococcus aureus atcc 29213 (0.03 to 0.12 μg/ml), enterococcus faecalis atcc 29212 (0.03 to 0.12 μg/ml), and streptococcus pneumoniae atcc 49619 (0.004 to 0.015 μg/ml). | 2014 | 24920777 |
what role do periodontal pathogens play in osteoarthritis and periprosthetic joint infections of the knee? | through the use of polymerase chain reaction (pcr)-electron spray ionization (esi)-time of flight (tof)-mass spectrometry (ms), we identified multiple periodontal pathogens within joint tissues of individuals undergoing replacement arthroplasties of the knee. the most prevalent of the periodontal pathogens were treponema denticola and enterococcus faecalis, the latter of which is commonly associated with apical periodontitis. these findings were unique to periprosthetic joint infections (pji) of ... | 2014 | 24921460 |
safety of topical vancomycin for pediatric spinal deformity: nontoxic serum levels with supratherapeutic drain levels. | retrospective cohort analysis. | 2014 | 24921849 |
influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities. | the results given in the literature are conflicting when considering the relationship between antimicrobial activity and chitosan characteristics. to be able to clarify, we prepared fifteen homogeneous chitosans with different acetylation degrees (da) and molecular weights (mw) by reacetylation of a fully deacetylated chitin under homogeneous conditions. they were tested at different ph values for their antimicrobial activities against four gram-negative bacteria (escherichia coli, pseudomonas a ... | 2014 | 24929684 |
impact of microbial cultures on proteolysis and release of bioactive peptides in fermented milk. | this study aimed at evaluating co-cultures of selected microorganisms for their proteolytic activity and capability to produce fermented milk enriched with ace-inhibitory (acei) peptides. selected yeasts (torulaspora delbruekii kl66a, galactomyces geotrichum kl20b, pichia kudriavzevii kl84a and kluyveromyces marxianus kl26a) and lactic acid bacteria strains (lactobacillus plantarum lat03, lb. plantarum klat01 and the not virulent enterococcus faecalis ke06) were screened as single cultures for t ... | 2014 | 24929726 |
the effect of different root canal medicaments on the elimination of enterococcus faecalis ex vivo. | the aim of this study was to evaluate the antimicrobial effect of chlorhexidine gel (chx-g) 2%, chlorhexidine powder (chx-p) 1%, povidone-iodine (pvp-i), polyhexanide and camphorated-and-mentholated chlorophenol (chkm) ex vivo. | 2013 | 24932119 |
antimicrobial efficiency of photodynamic therapy with different irradiation durations. | this study aimed to evaluate the antimicrobial efficiency of pdt and the effect of different irradiation durations on the antimicrobial efficiency of pdt. | 2013 | 24932123 |
effect of irrigation with tetraclean on bacterial leakage of obturated root canals. | the purpose of the study presented here was to evaluate the effect of tetraclean, hypoclean, chlor-xtra, 2% chlorhexidine and 6% sodium hypochlorite/17% edta as a final irrigant on bacterial leakage of the root canal. one hundred and fifty-five extracted human maxillary central incisors were randomly divided into five experimental groups of 25 teeth each and two control groups of 15 teeth each. the root canals in each group were irrigated with 2 ml of the relevant irrigant between each filing. t ... | 2014 | 24933773 |
microbial diversity in persistent root canal infections investigated by checkerboard dna-dna hybridization. | the aim of the present study was to investigate the composition of the root canal microbiota in endodontic failures in order to identify and quantify these microorganisms. | 2014 | 24935532 |
antibacterial and anti-biofilm activity of ah plus with chlorhexidine and cetrimide. | the use of root canal filling materials with antibacterial activity can be considered beneficial to reduce the remaining microorganisms in the root canal system, where enterococcus faecalis is often found, and prevent recurrent infection. the aim of this study was to evaluate the antimicrobial activity and capacity for inhibiting e. faecalis biofilm formation of ah plus, alone and mixed with chlorhexidine (chx), cetrimide (ctr), and combinations of the two. | 2014 | 24935547 |
oral administration of ec-12 increases the baseline gene expression of antiviral cytokine genes, ifn-γ and tnf-α, in splenocytes and mesenteric lymph node cells of weaning piglets. | weaning piglets are continuously exposed to various viruses. the antiviral effects of lactic acid bacteria (lab) have been confirmed mainly in humans and mice, while few studies have been conducted in livestock. in this study, we evaluated the effect of oral administration of enterococcus faecalis strain ec-12 (ec-12) on the gene expressions of antiviral cytokines in weaning piglets. piglets were allocated to the ec-12-administered group (e group) and the no-treatment control group (c group). th ... | 2013 | 24936371 |
oxidative stress enhances the expression of sulfur assimilation genes: preliminary insights on the enterococcus faecalis iron-sulfur cluster machinery regulation. | the firmicutes bacteria participate extensively in virulence and pathological processes. enterococcus faecalis is a commensal microorganism; however, it is also a pathogenic bacterium mainly associated with nosocomial infections in immunocompromised patients. iron-sulfur [fe-s] clusters are inorganic prosthetic groups involved in diverse biological processes, whose in vivo formation requires several specific protein machineries. escherichia coli is one of the most frequently studied microorganis ... | 2014 | 24936909 |
new intracanal formulations containing doxycycline or chlorhexidine against enterococcus faecalis. | the present study aims to evaluate the antimicrobial effect of two new intracanal preparations against e. faecalis. thirty single-rooted human canine teeth were used. the crowns were removed and the roots were instrumented using a conventional technique. three groups of ten teeth each were infected with 108 cfu/ ml of e. faecalis for 21 days. the root canals were flled with new intracanal medications containing 3% doxycycline hydrochloride (dx) or 2% chlorhexidine digluconate (chx). ten teeth re ... | 2014 | 24939266 |
the effect of experimental streptococcus infection in myocarditis on some biochemical and inflammatory markers in albino rats. | myocarditis is an uncommon disease that presents with a wide range of symptoms in children and adults. it is histologically characterized by varying degrees of myocardialnecrosis, edema and cellular infiltration myocardial inflammation is a nonspecificresponse to many triggers such as bacterial infection, cardio toxic agents, ormechanical injury. | 2013 | 24940333 |
bacteriology and drug susceptibility analysis of pus from patients with severe intra-abdominal infection induced by abdominal trauma. | the aim of the present study was to retrospectively analyze the bacteriology and drug susceptibility of pus flora from abdominal trauma patients with severe intra-abdominal infection (siai). a total of 41 patients with siai induced by abdominal trauma were enrolled in the study, from which 123 abdominal pus samples were obtained. the results from laboratory microbiology and drug sensitivity were subjected to susceptibility analysis using whonet software. a total of 297 strains were isolated in w ... | 2014 | 24940451 |
autoimmune neutropenia of infancy with recurrent urinary tract infections: a case report. | autoimmune neutropenia of infancy is characterized by minor intercurrent infections despite severe neutropenia; severe bacterial infections are uncommon. an infant developed recurrent urinary tract infections at 9 and 11 months of age. the identified uropathogens were escherichia coli and enterococcus faecalis, respectively. empirical treatment with carbapenems, as broad-spectrum antibiotics, promptly resolved the infection without sequelae. febrile neutropenic children with cancer and autoimmun ... | 2015 | 24942028 |
use of a dissolved oxygen microsensor for assessing the viability and thickness of microbial biofilm on root surfaces. | to evaluate the use of a dissolved oxygen microsensor (doms) for assessing the viability and thickness of microbial biofilms on the apical external surface of contaminated human tooth roots. | 2015 | 24942082 |
characteristics of prosthetic joint infections due to enterococcus sp. and predictors of failure: a multi-national study. | the objective of this study was to review the characteristics and outcome of prosthetic joint infections (pji) due to enterococcus sp. collected in 18 hospitals from six european countries. patients with a pji due to enterococcus sp. diagnosed between january 1999 and july 2012 were retrospectively reviewed. relevant information about demographics, comorbidity, clinical characteristics, microbiological data, surgical treatment and outcome was registered. univariable and multivariable analyses we ... | 2014 | 24943469 |
effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine. | the purpose of this in vitro study was to determine whether the addition of 2% chlorhexidine gluconate or 10% doxycycline would enhance the antimicrobial activity of biodentine against staphylococcus aureus (atcc-25923), enterococcus faecalis (atcc-29212), candida albicans (atcc-90028), and streptococcus mutans (mtcc-497). | 2014 | 24944453 |
poloxamer-based curcumin solid dispersions for ex tempore preparation of supersaturated solutions intended for antimicrobial photodynamic therapy. | abstract phototoxic effect of curcumin supersaturated solutions toward pathogenic bacteria has already been demonstrated. however, to be useful in the clinical practice, a supersaturated solution needs to be physically and chemically stabile over the relevant time period. poloxamer-based solid dispersions (sd) intended for ex tempore preparation of a supersaturated solution were designed to simultaneously facilitate dissolution and inhibit precipitation of curcumin in vitro. due to the transform ... | 2014 | 24946137 |
[photoreactivation of escherichia coli and enterococcus faecalis in the secondary effluent disinfected by uv-tio2]. | effects of photoreactivating light intensity (0-41 microw x cm(-2)) on photoreactivation of escherichia coli (e. coli) and enterococcus faecalis (e. faecalis) in the secondary effluent after uv and uv-tio2 disinfection were investigated. the results indicated that the disinfection efficiency of uv-tio2 was much higher than that of uv disinfection. the photoreactivation rate of e. coli was much higher in uv disinfection than that in uv-tio2 disinfection. under high light intensity in uv-tio2 disi ... | 2014 | 24946584 |
draft genome sequence of enterococcus faecalis mb5259. | in this study, we present a draft genome sequence of enterococcus faecalis mb5259, a promising probiotic strain. the identified differences and common features between this strain and reference strains will assist in better understanding the mechanism of antibacterial action and in developing novel probiotics. | 2014 | 24948775 |
endophytic fungi from myrcia guianensis at the brazilian amazon: distribution and bioactivity. | beneficial interactions between plants and microorganisms have been investigated under different ecological, physiological, biochemical, and genetic aspects. however, the systematic exploration of biomolecules with potential for biotechnological products from this interaction still is relatively scarce. therefore, this study aimed the evaluation of the diversity and antimicrobial activity of the endophytic fungi obtained from roots, stems and leafs of myrcia guianensis (myrtaceae) from the brazi ... | 2014 | 24948926 |