Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| inner workings: a big role for a microbial parasite. | 0 | 27095842 | |
| a novel xenomonitoring technique using mosquito excreta/feces for the detection of filarial parasites and malaria. | given the continued successes of the world's lymphatic filariasis (lf) elimination programs and the growing successes of many malaria elimination efforts, the necessity of low cost tools and methodologies applicable to long-term disease surveillance is greater than ever before. as many countries reach the end of their lf mass drug administration programs and a growing number of countries realize unprecedented successes in their malaria intervention efforts, the need for practical molecular xenom ... | 2016 | 27096156 |
| in vitro template-change pcr to create single crossover libraries: a case study with b. thuringiensis cry2a toxins. | during evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequence variation and function. however, no well-developed in vitro method has been established to create single-crossover libraries. here we present an in vitro template-change polymerase change reaction ... | 2016 | 27097519 |
| association between nutritional status and dengue infection: a systematic review and meta-analysis. | dengue infection has various clinical manifestations, often with unpredictable clinical evolutions and outcomes. several factors including nutritional status have been studied to find the relationship with dengue severity. however, the nutritional status had conflicting effects on the complication of dengue in some previous studies. therefore, we conducted a systematic review and performed a meta-analysis to analyze the association between nutritional status and the outcome of dengue infection. | 2016 | 27097934 |
| the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania. | lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ... | 2016 | 27098567 |
| development and validation of a quantitative, one-step, multiplex, real-time reverse transcriptase pcr assay for detection of dengue and chikungunya viruses. | dengue virus (denv) and chikungunya virus (chikv) are important human pathogens with common transmission vectors and similar clinical presentations. patient care may be impacted by the misdiagnosis of denv and chikv in areas where both viruses cocirculate. in this study, we have developed and validated a one-step multiplex reverse transcriptase pcr (rt-pcr) to simultaneously detect, quantify, and differentiate between four denv serotypes (pan-denv) and chikungunya virus. the assay uses taqman te ... | 2016 | 27098955 |
| depletion of alveolar macrophages does not prevent hantavirus disease pathogenesis in golden syrian hamsters. | andes virus (andv) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (hps). the mechanism for the massive vascular leakage associated with hps is poorly understood; however, dysregulation of components of the immune response is often suggested as a possible cause. alveolar macrophages are found in the alveoli of the lung and represent the first line of defense to many airborne pathogens. to determine whether alveolar macrophages play a role in hps ... | 2016 | 27099308 |
| no evidence for local adaptation of dengue viruses to mosquito vector populations in thailand. | despite their epidemiological importance, the evolutionary forces that shape the spatial structure of dengue virus genetic diversity are not fully understood. fine-scale genetic structure of mosquito vector populations and evidence for genotype × genotype interactions between dengue viruses and their mosquito vectors are consistent with the hypothesis that the geographical distribution of dengue virus genetic diversity may reflect viral adaptation to local mosquito populations. to test this hypo ... | 2016 | 27099625 |
| the mitotic spindle: linking teratogenic effects of zika virus with human genetics? | recently, an association between zika virus infection and microcephaly/ocular findings was found to be reasonable e.g. because of the demonstration that the virus was found in the brain of a fetus after presumed maternal infection. although there is no proof yet for a causal relationship, for an appropriate risk calculation efforts are urgently needed to either establish or disprove this assumption. | 2016 | 27099632 |
| epidemiology of emergent madariaga encephalitis in a region with endemic venezuelan equine encephalitis: initial host studies and human cross-sectional study in darien, panama. | neurotropic arboviral infections are an important cause of encephalitis. a zoonotic, vector-borne alphavirus, madariaga virus (madv; formerly known as south american eastern equine encephalitis virus), caused its first documented human outbreak in 2010 in darien, panama, where the genetically similar venezuelan equine encephalitis virus (veev) is endemic. we report the results of a seroprevalence survey of animals and humans, illustrating contrasting features of madv and veev ecology and epidemi ... | 2016 | 27101567 |
| an updated checklist of mosquito species (diptera: culicidae) from madagascar. | an updated checklist of 235 mosquito species from madagascar is presented. the number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). this annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. the 235 species belong to 14 genera: aedeomyia (3 species), aedes (35 species), anophel ... | 2016 | 27101839 |
| enhancement of aedes albopictus collections by ovitrap and sticky adult trap. | in the last decades, aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which dengue, chikungunya and zika. enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control ... | 2016 | 27102015 |
| infection pattern and transmission potential of chikungunya virus in two new world laboratory-adapted aedes aegypti strains. | chikungunya virus (chikv) is an emerging mosquito-borne virus belonging to the togaviridae, which is transmitted to humans by aedes aegypti and ae. albopictus. we describe the infection pattern of chikv in two new world ae. aegypti strains, hwe and orl. both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious addition ... | 2016 | 27102548 |
| age-stage, two-sex life table characteristics of aedes albopictus and aedes aegypti in penang island, malaysia. | the life table developmental attributes of laboratory colonies of wild strains of aedes albopictus and aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (r0), and mean generation time (t). the total preadult development time was 9.47 days for ae. albopictus and 8.76 days fo ... | 2016 | 27105211 |
| characteristics and efficacy of three commercial handheld thermal foggers with pyrethroid insecticides against three species of mosquitoes. | the field study's objectives were to compare the physical characteristics as well as efficacy with multiple insecticides for bonide(®) fog rx insect fogger, black flag(®) propane insect fogger, and burgess(®) outdoor propane insect fogger. evaluations were conducted with 7 machine chemical combinations, 3 depths of spray, and 3 species of laboratory-reared mosquitoes, anopheles quadrimaculatus, culex quinquefasciatus, and aedes aegypti. combinations of these factors were analyzed in conjunction ... | 2016 | 27105215 |
| reproductive incompatibility involving senegalese aedes aegypti (l) is associated with chromosome rearrangements. | aedes aegypti, the primary vector of dengue, yellow fever and zika flaviviruses, consists of at least two subspecies. aedes aegypti (aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. aedes aegypti formosus (aaf), has a dark cuticle, is restricted to sub-saharan africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. scale patterns correlate with cuticle ... | 2016 | 27105225 |
| inferring the spatio-temporal patterns of dengue transmission from surveillance data in guangzhou, china. | dengue is a serious vector-borne disease, and incidence rates have significantly increased during the past few years, particularly in 2014 in guangzhou. the current situation is more complicated, due to various factors such as climate warming, urbanization, population increase, and human mobility. the purpose of this study is to detect dengue transmission patterns and identify the disease dispersion dynamics in guangzhou, china. | 2016 | 27105350 |
| development of a sybr green i-based quantitative rt-pcr for ross river virus: application in vector competence studies and antiviral drug evaluation. | ross river virus (rrv) is an emerging alphavirus and is presently endemic in many parts of oceania. keeping in mind its emergence, we developed a molecular detection system and utilized it to study vector competence and evaluate activity of antiviral compounds against rrv. a sybr green i-based quantitative rt-pcr for detection of rrv was developed targeting the e2 gene, with a detection limit of 100 rna copies/reaction. the specificity was confirmed with closely related alphaviruses and flavivir ... | 2016 | 27105737 |
| sequence capture by hybridization to explore modern and ancient genomic diversity in model and nonmodel organisms. | the recent expansion of next-generation sequencing has significantly improved biological research. nevertheless, deep exploration of genomes or metagenomic samples remains difficult because of the sequencing depth and the associated costs required. therefore, different partitioning strategies have been developed to sequence informative subsets of studied genomes. among these strategies, hybridization capture has proven to be an innovative and efficient tool for targeting and enriching specific b ... | 2016 | 27105841 |
| seasonality and the effectiveness of mass vaccination. | many infectious diseases have seasonal outbreaks, which may be driven by cyclical environmental conditions (e.g., an annual rainy season) or human behavior (e.g., school calendars or seasonal migration). if a pathogen is only transmissible for a limited period of time each year, then seasonal outbreaks could infect fewer individuals than expected given the pathogen's in-season transmissibility. influenza, with its short serial interval and long season, probably spreads throughout a population un ... | 0 | 27105983 |
| effects of larval nutrition on wolbachia-based dengue virus interference in aedes aegypti (diptera: culicidae). | in order to assess the broad-scale applicability of field releases of wolbachia for the biological control of insect-transmitted diseases, we determined the relationship between the larval diet of aedes aegypti l. mosquitoes infected with wolbachia strains and their susceptibility to dengue virus (denv) infection via intrathoracic injection and oral inoculation. larvae were reared on diets that varied in the quantity of food which had the effect of modifying development time and adult body size. ... | 2016 | 27106932 |
| vertical transmission of indian ocean lineage of chikungunya virus in aedes aegypti and aedes albopictus mosquitoes. | the re-emergence of chikungunya (chik) fever in thailand has been caused by a novel lineage of chikungunya virus (chikv) termed the indian ocean lineage (iol). the aedes albopictus mosquito is thought to be a primary vector of chik fever in thailand, whereas ae. aegypti acts as a secondary vector of the virus. the vertical transmission is believed to be a primary means to maintain chikv in nature and may be associated with an increased risk of outbreak. therefore, the goal of this study was to a ... | 2016 | 27108077 |
| analysis of protein composition and bioactivity of neoponera villosa venom (hymenoptera: formicidae). | ants cause a series of accidents involving humans. such accidents generate different reactions in the body, ranging from a mild irritation at the bite site to anaphylactic shock, and these reactions depend on the mechanism of action of the venom. the study of animal venom is a science known as venomics. through venomics, the composition of the venom of several ant species has already been characterized and their biological activities described. thus, the aim of this study was to evaluate the pro ... | 2016 | 27110765 |
| knowledge, attitudes, and practices of florida physicians regarding dengue before and after an educational intervention. | failure to recognize and appropriately manage dengue early in the clinical course may result in late initiation of supportive treatment for severe disease. in florida, travel-related and autochthonous dengue occur and are likely under-recognized. the objective of this study was to evaluate physician knowledge of dengue and its management before and after an educational intervention in florida. | 2016 | 27112138 |
| prevalence of dengue and chikungunya virus infections in north-eastern tanzania: a cross sectional study among participants presenting with malaria-like symptoms. | in spite of increasing reports of dengue and chikungunya activity in tanzania, limited research has been done to document the general epidemiology of dengue and chikungunya in the country. this study aimed at determining the sero-prevalence and prevalence of acute infections of dengue and chikungunya virus among participants presenting with malaria-like symptoms (fever, headache, rash, vomit, and joint pain) in three communities with distinct ecologies of north-eastern tanzania. | 2016 | 27112553 |
| recent changes in the local distribution of aedes aegypti (diptera: culicidae) in south florida, usa. | disease transmission is directly tied to the spatial distribution of disease vectors. the distribution of aedes aegypti (l.) in the united states has diminished since the introduction of aedes albopictus (skuse) in the 1980s. however, ae. aegypti persists in some urban areas, particularly in south florida. the pattern of habitat segregation of these two species is well documented, but the consistency of this phenomenon over time is unknown. to examine the dynamics of the local distributions of t ... | 2016 | 27113103 |
| detection and distribution of v1016ikdr mutation in the voltage-gated sodium channel gene in aedes aegypti (diptera: culicidae) populations from sergipe state, northeast brazil. | aedes aegypti (l.) resistance to pyrethroids was recorded in brazil few years after its introduction as the adulticide in the national dengue control program campaigns. altered susceptibility to pyrethroids had been reported in the state of sergipe, northeast brazil, through biological assays, even before its use against ae. aegypti in the state. metabolic and target-site resistance mechanisms were also revealed in samples from aracaju, the capital of sergipe. herein, we investigated the presenc ... | 2016 | 27113110 |
| insights into the evolution of chemoreceptor genes superfamily in tyrophagus putrescentiae (acari: acaridae). | all living organisms, including animals, plants, fungi, and bacteria, use the olfactory system to recognize chemicals or pheromone from their environment. insects detect a volatile substance using odorant receptors (ors) or gustatory receptors (grs) and ionotropic receptors (irs). the gene families of the olfactory system in acari are still not clear. in this study, we identified seven ors, one gr, and five irs from the transcriptome of the storage mite, tyrophagus putrescentiae schrank. no olfa ... | 2016 | 27113112 |
| pfsmad4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster pinctada fucata. | mollusca is the second largest phylum in nature. the shell of molluscs is a remarkable example of a natural composite biomaterial. biomineralization and how it affects mollusks is a popular research topic. the bmp-2 signaling pathway plays a canonical role in biomineralization. smad4 is an intracellular transmitter in the bmp signaling pathway in mammals, and some genomic data show smad4's involvement in bmp signaling in invertebrates, but whether smad4 plays a conservative role in pearl oyster, ... | 2016 | 27113217 |
| characterization of drosophila cmp-sialic acid synthetase activity reveals unusual enzymatic properties. | cmp-sialic acid synthetase (csas) is a key enzyme of the sialylation pathway. csas produces the activated sugar donor, cmp-sialic acid, which serves as a substrate for sialyltransferases to modify glycan termini with sialic acid. unlike other animal csass that normally localize in the nucleus, drosophila melanogaster csas (dmcsas) localizes in the cell secretory compartment, predominantly in the golgi, which suggests that this enzyme has properties distinct from those of its vertebrate counterpa ... | 2016 | 27114558 |
| zika virus: a global threat to humanity: a comprehensive review and current developments. | at present, one of greatest concerns of medical personnel is zika virus (zikv). though it has been reported for quite a long time, its rapid emergence, new modes of transmission, and more importantly, the congenital anomalies associated with it have made the situation worse. it was first detected in 1947. after that, this infection was found in the countries of africa as well as asia. at present, interestingly it has been reported from brazil. microcephaly and intracranial calcification have bee ... | 0 | 27114968 |
| spread of the invasive mosquitoes aedes aegypti and aedes albopictus in the black sea region increases risk of chikungunya, dengue, and zika outbreaks in europe. | 2016 | 27115737 | |
| production of dengue virus envelope protein domain iii-based antigens in tobacco chloroplasts using inducible and constitutive expression systems. | dengue fever is a disease in many parts of the tropics and subtropics and about half the world's population is at risk of infection according to the world health organization. dengue is caused by any of the four related dengue virus serotypes den-1, -2, -3 and -4, which are transmitted to people by aedes aegypti mosquitoes. currently there is only one vaccine (dengvaxia(®)) available (limited to a few countries) on the market since 2015 after half a century's intensive efforts. affordable and ac ... | 2016 | 27116001 |
| droplet characterization and penetration of an ultra-low volume mosquito adulticide spray targeting the asian tiger mosquito, aedes albopictus, within urban and suburban environments of northeastern usa. | adult control of aedes albopictus via ultra-low volume is difficult because this species occurs primarily in peridomestic habitats where obstacles such as buildings and vegetation can disrupt spray plumes and droplet dispersion. we determined droplet penetration and characterization of a pyrethroid adulticide applied from the ground at mid (46.77 ml/ha) and maximum (93.53 ml/ha) label rates within cryptic habitats of urban and suburban environments. droplets were collected from all habitats, wit ... | 2016 | 27116103 |
| the larvicidal effects of black pepper (piper nigrum l.) and piperine against insecticide resistant and susceptible strains of anopheles malaria vector mosquitoes. | insecticide resistance carries the potential to undermine the efficacy of insecticide based malaria vector control strategies. therefore, there is an urgent need for new insecticidal compounds. black pepper (dried fruit from the vine, piper nigrum), used as a food additive and spice, and its principal alkaloid piperine, have previously been shown to have larvicidal properties. the aim of this study was to investigate the larvicidal effects of ground black pepper and piperine against third and fo ... | 2016 | 27117913 |
| excretion of dengue virus rna by aedes aegypti allows non-destructive monitoring of viral dissemination in individual mosquitoes. | successful transmission of a vector-borne pathogen relies on a complex life cycle in the arthropod vector that requires initial infection of the digestive tract followed by systemic viral dissemination. the time interval between acquisition and subsequent transmission of the pathogen, called the extrinsic incubation period, is one of the most influential parameters of vector-borne pathogen transmission. however, the dynamic nature of this process is often ignored because vector competence assays ... | 2016 | 27117953 |
| de novo adult transcriptomes of two european brittle stars: spotlight on opsin-based photoreception. | next generation sequencing (ngs) technology allows to obtain a deeper and more complete view of transcriptomes. for non-model or emerging model marine organisms, ngs technologies offer a great opportunity for rapid access to genetic information. in this study, paired-end illumina hiseqtm technology has been employed to analyse transcriptomes from the arm tissues of two european brittle star species, amphiura filiformis and ophiopsila aranea. about 48 million illumina reads were generated and 136 ... | 2016 | 27119739 |
| rhodnius prolixus and r. robustus (hemiptera: reduviidae) nymphs show different locomotor patterns on an automated recording system. | circadian rhythms of triatomines, vectors of the etiological agent trypanosoma cruzi responsible for chagas disease, have been extensively studied in adults of the two most epidemiologically relevant vector species, rhodnius prolixus and triatoma infestans. however, little attention has been dedicated to the activity patterns in earlier developmental stages, even though triatomine nymphs are equally capable of transmitting t. cruzi to humans. because circadian rhythms may differ even between clo ... | 2016 | 27121502 |
| international air travel to ohio, usa, and the impact on malaria, influenza, and hepatitis a. | the state of ohio led the united states in measles in 2014, ostensibly related to international air travel (iat), and ranked lower than 43 other states in infectious disease outbreak preparedness. we conducted a retrospective cohort study using surveillance data of the total ohio population of 11 million from 2010 through 2014 with a nested case control of air travelers to determine the risk of malaria, seasonal influenza hospitalizations (ih), and hepatitis a (ha) disease related to internation ... | 2016 | 27123365 |
| analogs of ldl receptor ligand motifs in dengue envelope and capsid proteins as potential codes for cell entry. | it is established that cell entry of low density lipoprotein particles (llps) containing apo b100 and apo e is mediated by receptors and gags. receptor ligand motifs, xbbbxxbx, xbbxbx, and ψbψxb, and mono- and bipartite nls sequences are abundant in apo e and apo b100 as well as in envelope and capsid proteins of dengue viruses 1-4 (denv1-4). synthetic, fluorescence-labeled peptides of sequences in denv2 envelope protein, and denv3 capsid that include these motifs were used to conduct a qualitat ... | 0 | 27123468 |
| zika virus: an update on epidemiology, pathology, molecular biology, and animal model. | zika virus (zikv) was first described in 1947, and became a health emergency problem in 2016 when its association with fetal microcephaly cases was confirmed by centers for disease control and prevention (cdc) in the united states. to date, zikv infection has been documented in 66 countries. zikv is recognized as a neurotropic virus and numerous diseases manifested in multiple neurological disorders have been described, mainly in countries that have been exposed to zikv after the 2007 outbreak i ... | 2016 | 27124623 |
| the wmel strain of wolbachia reduces transmission of chikungunya virus in aedes aegypti. | new approaches to preventing chikungunya virus (chikv) are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival. a promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium wolbachia into aedes aegypti mosquitoes. this primarily has been proposed as a tool to control dengue viru ... | 2016 | 27124663 |
| phytochemical composition, antifungal and antioxidant activity of duguetia furfuracea a. st.-hill. | background. duguetia furfuracea is popular plant used in popular medicine. hypothesis/purpose. this claim evaluated the phytochemical composition of the hydroethanolic extract (hedf), fractions of duguetia furfuracea, and antioxidant and antifungal activity. methods. the chemical profile was carried out by hplc-dad. the total phenolic contents and flavonoid components were determined by folin-ciocalteu and aluminium chloride reaction. the antioxidant activity was measured by scavenging of 2,2-di ... | 2016 | 27127550 |
| predicting dengue fever outbreaks in french guiana using climate indicators. | dengue fever epidemic dynamics are driven by complex interactions between hosts, vectors and viruses. associations between climate and dengue have been studied around the world, but the results have shown that the impact of the climate can vary widely from one study site to another. in french guiana, climate-based models are not available to assist in developing an early warning system. this study aims to evaluate the potential of using oceanic and atmospheric conditions to help predict dengue f ... | 2016 | 27128312 |
| how relevant is panallergen sensitization in the development of allergies? | panallergens comprise various protein families of plant as well as animal origin and are responsible for wide ige cross-reactivity between related and unrelated allergenic sources. such cross-reactivities include reactions between various pollen sources, pollen and plant-derived foods as well as invertebrate-derived inhalants and foodstuff. here, we provide an overview on the most clinically relevant panallergens from plants (profilins, polcalcins, non-specific lipid transfer proteins, pathogene ... | 0 | 27129102 |
| the risk of dengue for non-immune foreign visitors to the 2016 summer olympic games in rio de janeiro, brazil. | rio de janeiro in brazil will host the summer olympic games in 2016. about 400,000 non-immune foreign tourists are expected to attend the games. as brazil is the country with the highest number of dengue cases worldwide, concern about the risk of dengue for travelers is justified. | 2016 | 27129407 |
| erratum to: co-distribution and co-infection of chikungunya and dengue viruses. | 2016 | 27129475 | |
| multiple qtl determine dorsal abdominal scale patterns in the mosquito aedes aegypti. | the mosquito, aedes aegypti (l.) originated in sub-saharan africa as a dark form sylvan species (a. aegypti formosus). evolution of a. aegypti aegypti type form as a human commensal facilitated its colonization of most semitropical and tropical areas. we investigated the genetic basis for abdominal white scale presence that represents the diagnostic for sylvan a. aegypti formosus (scales absent), from type form (scales present) and a. aegypti queenslandensis form (dense scaling). we performed qu ... | 2016 | 27130203 |
| dengue virus immunopathogenesis: lessons applicable to the emergence of zika virus. | dengue is the leading mosquito-transmitted viral infection in the world. there are more than 390 million new infections annually; while the majority of infected individuals are asymptomatic or develop a self-limited dengue fever, up to 1 million clinical cases develop severe manifestations, including dengue hemorrhagic fever and shock syndrome, resulting in ~25,000 deaths annually, mainly in children. gaps in our understanding of the mechanisms that contribute to dengue infection and immunopatho ... | 2016 | 27130436 |
| first identification of kdr allele f1534s in vgsc gene and its association with resistance to pyrethroid insecticides in aedes albopictus populations from haikou city, hainan island, china. | aedes albopictus is distributed widely in china, as a primary vector of dengue fever and chikungunya fever in south of china. chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases. long-term applications of pyrethroids have resulted in the development of resistance in ae. albopictus populations in china. however, the susceptibility of ae. albopictus to pyrethroids in hainan island was unclear. knockdown resistance (kdr), caused by point mutations in ... | 2016 | 27133234 |
| zika virus infections imported from brazil to portugal, 2015. | zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ... | 2016 | 27134823 |
| molecular characterisation of clinical isolates of chikungunya virus: a study from tertiary care hospitals in southern india. | indian ocean islands and india have experienced massive severe chikungunya outbreak from 2005 up till now and then chikungunya became epidemic in india. the mutations that occurred in e1 gene were responsible for increased infectivity, virulence and host adaptability. it is important to find out the genotype and its probable evolvement and novel mutations in the e1 gene reported during 2006-2009 from the current isolates, which may affect the local protein structure. | 2016 | 27134872 |
| the p450 cyp6z1 confers carbamate/pyrethroid cross-resistance in a major african malaria vector beside a novel carbamate-insensitive n485i acetylcholinesterase-1 mutation. | carbamates are increasingly used for vector control notably in areas with pyrethroid resistance. however, a cross-resistance between these insecticides in major malaria vectors such as anopheles funestus could severely limit available resistance management options. unfortunately, the molecular basis of such cross-resistance remains uncharacterized in an. funestus, preventing effective resistance management. here, using a genomewide transcription profiling, we revealed that metabolic resistance t ... | 2016 | 27135886 |
| sequence conservation and sexually dimorphic expression of the ftz-f1 gene in the crustacean daphnia magna. | identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. orthologs of drosophila orphan receptor fushi tarazu factor-1 (ftz-f1) are known to function in genetic sex determination. in contrast, their roles in environmental sex determination remain unknown. in this study, we have cloned and characterized the ftz-f1 ortholog in the branchiopod crustacean daphnia magna, which produces males in ... | 2016 | 27138373 |
| identification and characterization of two novel rna viruses from anopheles gambiae species complex mosquitoes. | mosquitoes of the anopheles gambiae complex display strong preference for human bloodmeals and are major malaria vectors in africa. however, their interaction with viruses or role in arbovirus transmission during epidemics has been little examined, with the exception of o'nyong-nyong virus, closely related to chikungunya virus. deep-sequencing has revealed different rna viruses in natural insect viromes, but none have been previously described in the anopheles gambiae species complex. here, we d ... | 2016 | 27138938 |
| molecular characterization of two major dengue outbreaks in costa rica. | dengue virus (denv) (flavivirus, flaviviridae) is a reemerging arthropod-borne virus with a worldwide circulation, transmitted mainly by aedes aegypti and aedes albopictus mosquitoes. since the first detection of its main transmitting vector in 1992 and the invasion of denv-1 in 1993, costa rica has faced dengue outbreaks yearly. in 2007 and 2013, costa rica experienced two of the largest outbreaks in terms of total and severe cases. to provide genetic information about the etiologic agents prod ... | 2016 | 27139442 |
| impact of inter- and intra-specific competition among larvae on larval, adult, and life-table traits of aedes aegypti and aedes albopictus females. | few studies have taken a comprehensive approach of measuring the impact of inter- and intra-specific larval competition on adult mosquito traits. in this study, the impact of competition aedes aegypti and a. albopictus was quantified over the entire life of a cohort.competitive treatments affected hatch-to-adult survivorship and development time to adulthood of females for both species, but affected median wing length of females only for a. albopictus. competitive treatments had no significant e ... | 2016 | 27141149 |
| differential gene expression analysis of the epacromius coerulipes (orthoptera: acrididae) transcriptome. | epacromius coerulipes (ivanov) is one of the most widely distributed locusts. to date, the main methods to kill locusts still rely on chemical controls, which can result in the selection of locusts with resistance to chemical pesticides. butene-fipronil is a new pesticide that was discovered by the structural modification of fipronil. this pesticide has been used to control various agricultural pests and has become an important pesticide product to control pests that exhibit resistance to other ... | 2016 | 27142308 |
| optimal repellent usage to combat dengue fever. | dengue fever is one of the most important vector-borne diseases. it is transmitted by aedes stegomyia aegypti, and one of the most effective strategies to combat the disease is the reduction of exposure to bites of these mosquitoes. in this paper, we present a game-theoretical model in which individuals choose their own level of protection against mosquito bites in order to maximize their own benefits, effectively balancing the cost of protection and the risk of contracting the dengue fever. we ... | 2016 | 27142427 |
| overview of zika virus (zikv) infection in regards to the brazilian epidemic. | zika virus (zikv), a mosquito-borne flavivirus, belongs to the flaviviridae family, genus flavivirus. zikv was initially isolated in 1947 from a sentinel monkey in the zika forest, uganda. little clinical importance was attributed to zikv, once only few symptomatic cases were reported in some african and southeast asiatic countries. this situation changed in 2007, when a large outbreak was registered on the yap island, micronesia, caused by the asian zikv lineage. between 2013 and 2014, zikv spr ... | 2016 | 27143174 |
| spatial and temporal country-wide survey of temephos resistance in brazilian populations of aedes aegypti. | the organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (aedes aegypti) in brazil since the mid-1980s. reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. as resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in ae. aegypti in br ... | 2016 | 27143489 |
| chikungunya fever cases identified in the veterans health administration system, 2014. | during december 2013, the first locally transmitted chikungunya virus (chikv) infections in the americas were reported in the caribbean. although chikv infection is rarely fatal, risk for severe disease increases with age and medical comorbidities. herein we describe characteristics of veterans health administration (vha) patients with chikv infection and, among those with infections diagnosed in puerto rico, investigated risk factors for hospitalization. | 2016 | 27144588 |
| imbalanced hemolymph lipid levels affect feeding motivation in the two-spotted cricket, gryllus bimaculatus. | insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. adipokinetic hormone (akh) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. we recently demonstrated that rna interference (rnai)-mediated knockdown of the akh receptor (akhr) reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, gr ... | 2016 | 27144650 |
| vector competence of aedes aegypti and aedes polynesiensis populations from french polynesia for chikungunya virus. | from october 2014 to march 2015, french polynesia experienced for the first time a chikungunya outbreak. two aedes mosquitoes may have contributed to chikungunya virus (chikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito. | 2016 | 27144888 |
| ionotropic chemosensory receptors mediate the taste and smell of polyamines. | the ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. polyamine deficiency or excess has detrimental effects on health, cognitive function, reproduction, and lifespan. here, we show that a diet high in polyamine is beneficial and increases reproductive success of flies, and ... | 2016 | 27145030 |
| hawkmoths evaluate scenting flowers with the tip of their proboscis. | pollination by insects is essential to many ecosystems. previously, we have shown that floral scent is important to mediate pollen transfer between plants (kessler et al., 2015). yet, the mechanisms by which pollinators evaluate volatiles of single flowers remained unclear. here, nicotiana attenuata plants, in which floral volatiles have been genetically silenced and its hawkmoth pollinator, manduca sexta, were used in semi-natural tent and wind-tunnel assays to explore the function of floral sc ... | 2016 | 27146894 |
| a mosquito sperm's journey from male ejaculate to egg: mechanisms, molecules, and methods for exploration. | the fate of mosquito sperm in the female reproductive tract has been addressed sporadically and incompletely, resulting in significant gaps in our understanding of sperm-female interactions that ultimately lead to fertilization. as with other diptera, mosquito sperm have a complex journey to their ultimate destination, the egg. after copulation, sperm spend a short time at the site of insemination where they are hyperactivated and quickly congregate near the entrance of the spermathecal ducts. w ... | 0 | 27147424 |
| public health round-up. | 2016 | 27147758 | |
| zika virus: the latest newcomer. | since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: west nile, influenza a, avian flu, dengue, chikungunya, sars, mers, ebola, and now zika, the latest newcomer. zika virus (zikv), a flavivirus transmitted by aedes mosquitoes, was identified in 1947 in a sentinel monkey in uganda, and later on in humans in nigeria. the virus was mainly confined to the african continent until it was detected in south-east asia the 1980's, t ... | 2016 | 27148186 |
| zika virus: a new animal model for an arbovirus. | 2016 | 27149628 | |
| δ-cadinene, calarene and .δ-4-carene from kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes. | mosquitoes (diptera: culicidae) are major vectors of important pathogens and parasites. malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis cause millions of deaths every year. mosquito control is being challenging due to the development of pesticide resistance and negative environmental concerns. in this scenario, plants employed in traditional asian medicine may be alternative sources of newer and effective mosquitocides. in this research, we evaluated th ... | 2016 | 27151483 |
| management and treatment of dengue and chikungunya - natural products to the rescue. | neglected tropical diseases (ntds) flourish mostly in impoverished developing nations of the world. it is estimated that ntds plague up to 1 billion people every year thereby inducing a massive economic and health burden worldwide. following explosive outbreaks mostly in asia, latin america, europe and the indian ocean, two common ntds namely, chikungunya and dengue both transmitted by an infected mosquito vector principally aedes aegypti have emerged as a major public health threat. given the l ... | 2016 | 27151484 |
| inhibition and larvicidal activity of phenylpropanoids from piper sarmentosum on acetylcholinesterase against mosquito vectors and their binding mode of interaction. | aedes aegypti, aedes albopictus and culex quinquefasciatus are vectors of dengue fever and west nile virus diseases. this study was conducted to determine the toxicity, mechanism of action and the binding interaction of three active phenylpropanoids from piper sarmentosum (piperaceae) toward late 3rd or early 4th larvae of above vectors. a bioassay guided-fractionation on the hexane extract from the roots of piper sarmentosum led to the isolation and identification of three active phenylpropanoi ... | 2016 | 27152416 |
| midgut transcriptome of the cockroach periplaneta americana and its microbiota: digestion, detoxification and oxidative stress response. | the cockroach, periplaneta americana, is an obnoxious and notorious pest of the world, with a strong ability to adapt to a variety of complex environments. however, the molecular mechanism of this adaptability is mostly unknown. in this study, the genes and microbiota composition associated with the adaptation mechanism were studied by analyzing the transcriptome and 16s rdna pyrosequencing of the p. americana midgut, respectively. midgut transcriptome analysis identified 82,905 unigenes, among ... | 2016 | 27153200 |
| disease vectors in the era of next generation sequencing. | almost 20 % of all infectious human diseases are vector borne and, together, are responsible for over one million deaths per annum. over the past decade, the decreasing costs of massively parallel sequencing technologies have facilitated the agnostic interrogation of insect vector genomes, giving medical entomologists access to an ever-expanding volume of high-quality genomic and transcriptomic data. in this review, we highlight how genomics resources have provided new insights into the physiolo ... | 2016 | 27154554 |
| wolbachia blocks currently circulating zika virus isolates in brazilian aedes aegypti mosquitoes. | the recent association of zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the zika vector, aedes aegypti mosquitoes. wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. here we report that aedes aegypti harboring wolbachia are highly resistant to infection with two currently circulating zika virus isolates from the recent brazilian epidemic. wolbac ... | 2016 | 27156023 |
| [zika virus infection or the future of infectious diseases]. | zika virus belongs to the flaviridae, an extended phylogenetic family containing dengue or yellow fever, viruses whose shared main vector are aedes aegypti mosquitoes. the virus originally came from central african simian reservoirs and, from there, expanded rapidly across the pacific to south america. the disease is an example of exantematic fever usually mild. mortality is very low and mainly limited to secondary guillain-barré or fetal microcephaly cases. diagnostic confirmation requires a rt ... | 2016 | 27156484 |
| yellow fever cases in asia: primed for an epidemic. | there is currently an emerging outbreak of yellow fever in angola. cases in infected travellers have been reported in a number of other african countries, as well as in china, representing the first ever documented cases of yellow fever in asia. there is a large chinese workforce in angola, many of whom may be unvaccinated, increasing the risk of ongoing importation of yellow fever into asia via busy commercial airline routes. large parts of the region are hyperendemic for the related flavivirus ... | 2016 | 27156836 |
| surveillance, insecticide resistance and control of an invasive aedes aegypti (diptera: culicidae) population in california. | the invasion and subsequent establishment in california of aedes aegypti in 2013 has created new challenges for local mosquito abatement and vector control districts. studies were undertaken to identify effective and economical strategies to monitor the abundance and spread of this mosquito species as well as for its control. overall, bg sentinel (bgs) traps were found to be the most sensitive trap type to measure abundance and spread into new locations. autocidal-gravid-ovitraps (ago-b), when p ... | 2016 | 27158450 |
| clinical and epidemiological characterization of laboratory-confirmed autochthonous cases of zika virus disease in mexico. | since 2014, autochthonous circulation of zika virus (zikv) in the americas was detected (easter island, chile). in may 2015, brazil confirmed autochthonous --transmission and in october of that year colombia reported their first cases. now more than 52 countries have reported cases, including mexico. to deal with this contingency in mexico, several surveillance systems, in addition to systems for vector-borne diseases were strengthened with the participation of all health institutions. also, th ... | 2016 | 27158557 |
| zika virus - emergence, evolution, pathology, diagnosis, and control: current global scenario and future perspectives - a comprehensive review. | this review converses the zika virus which has attained global concern due to its rapid pandemic potential and impact on humans. though zika virus was first isolated in 1947, till the recent large-scale outbreak which occurred in micronesia, in 2007, the virus was placed into the innocuous pathogen category. the world health organization on 1 february 2016 declared it as a 'public health emergency of international concern.' of the note, american as well as pacific island strains/isolates is rela ... | 2016 | 27158761 |
| diversity, evolution and medical applications of insect antimicrobial peptides. | antimicrobial peptides (amps) are short proteins with antimicrobial activity. a large portion of known amps originate from insects, and the number and diversity of these molecules in different species varies considerably. insect amps represent a potential source of alternative antibiotics to address the limitation of current antibiotics, which has been caused by the emergence and spread of multidrug-resistant pathogens. to get more insight into amps, we investigated the diversity and evolution o ... | 2016 | 27160593 |
| high-throughput cis-regulatory element discovery in the vector mosquito aedes aegypti. | despite substantial progress in mosquito genomic and genetic research, few cis-regulatory elements (cres), dna sequences that control gene expression, have been identified in mosquitoes or other non-model insects. formaldehyde-assisted isolation of regulatory elements paired with dna sequencing, faire-seq, is emerging as a powerful new high-throughput tool for global cre discovery. faire results in the preferential recovery of open chromatin dna fragments that are not bound by nucleosomes, an ev ... | 2016 | 27161480 |
| the viral polymerase inhibitor 7-deaza-2'-c-methyladenosine is a potent inhibitor of in vitro zika virus replication and delays disease progression in a robust mouse infection model. | zika virus (zikv) is an emerging flavivirus typically causing a dengue-like febrile illness, but neurological complications, such as microcephaly in newborns, have potentially been linked to this viral infection. we established a panel of in vitro assays to allow the identification of zikv inhibitors and demonstrate that the viral polymerase inhibitor 7-deaza-2'-c-methyladenosine (7dma) efficiently inhibits replication. infection of ag129 (ifn-α/β and ifn-γ receptor knock-out) mice with zikv res ... | 2016 | 27163257 |
| physicians, primary caregivers and topical repellent: all under-utilised resources in stopping dengue virus transmission in affected households. | primary health care facilities frequently manage dengue cases on an ambulatory basis for the duration of the patient's illness. there is a great opportunity for specific messaging, aimed to reduce dengue virus (denv) transmission in and around the home, to be directly targeted toward this high-risk ambulatory patient group, as part of an integrated approach to dengue management. the extent however, to which physicians understand, and can themselves effectively communicate strategies to stop foca ... | 2016 | 27164168 |
| the persistence and ecological impacts of a cyanobacterium genetically engineered to express mosquitocidal bacillus thuringiensis toxins. | the cyanobacterium anabaena pcc 7120#11 has been genetically engineered to act as a delivery vehicle for bacillus thuringiensis subspecies israelensis mosquitocidal toxins. to address ecological concerns about releasing this genetically engineered microorganism into the environment for mosquito larva control, the persistence and ecological impacts of pcc 7120#11 was evaluated using multi-species, standardized aquatic microcosms. | 2016 | 27165108 |
| an antibody against an anopheles albimanus midgut myosin reduces plasmodium berghei oocyst development. | malaria parasites are transmitted by anopheles mosquitoes. although several studies have identified mosquito midgut surface proteins that are putatively important for plasmodium ookinete invasion, only a few have characterized these protein targets and demonstrated transmission-blocking activity. molecular information about these proteins is essential for the development of transmission-blocking vaccines (tbv). the aim of the present study was to test three monoclonal antibodies (mabs), a-140, a ... | 2016 | 27165123 |
| comprehensive evaluation of candidate reference genes for qrt-pcr studies of gene expression in mustard aphid, lipaphis erysimi (kalt). | mustard aphid, also known as turnip aphid (lipaphis erysimi) is a major insect pest of rapeseed-mustard group of crops. tremendous economic significance has led to substantial basic research involving gene-expression studies in this insect species. in qrt-pcr analysis of gene-expression, normalization of data against rna variation by using appropriate reference gene is fundamental. however, appropriate reference genes are not known in case of l. erysimi. we evaluated 11 candidate reference genes ... | 2016 | 27165720 |
| myosin myth4-ferm structures highlight important principles of convergent evolution. | myosins containing myth4-ferm (myosin tail homology 4-band 4.1, ezrin, radixin, moesin, or mf) domains in their tails are found in a wide range of phylogenetically divergent organisms, such as humans and the social amoeba dictyostelium (dd). interestingly, evolutionarily distant mf myosins have similar roles in the extension of actin-filled membrane protrusions such as filopodia and bind to microtubules (mt), suggesting that the core functions of these mf myosins have been highly conserved over ... | 2016 | 27166421 |
| de novo transcriptome analysis and detection of antimicrobial peptides of the american cockroach periplaneta americana (linnaeus). | cockroaches are surrogate hosts for microbes that cause many human diseases. in spite of their generally destructive nature, cockroaches have recently been found to harbor potentially beneficial and medically useful substances such as drugs and allergens. however, genomic information for the american cockroach (periplaneta americana) is currently unavailable; therefore, transcriptome and gene expression profiling is needed as an important resource to better understand the fundamental biological ... | 2016 | 27167617 |
| biotype characterization, developmental profiling, insecticide response and binding property of bemisia tabaci chemosensory proteins: role of csp in insect defense. | chemosensory proteins (csps) are believed to play a key role in the chemosensory process in insects. sequencing genomic dna and rna encoding csp1, csp2 and csp3 in the sweet potato whitefly bemisia tabaci showed strong variation between b and q biotypes. analyzing csp-rna levels showed not only biotype, but also age and developmental stage-specific expression. interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in b and q young adults s ... | 2016 | 27167733 |
| evaluation of mosquito repellent activity of isolated oleic acid, eicosyl ester from thalictrum javanicum. | to evaluate the traditional use, the mosquito repellent property of thalictrum javanicum and to confirm the predicted larvicidal activity of the isolated compound, oleic acid, eicosyl ester from its aerial parts by pass software, the present study was carried out using 4th instar stage larvae of the mosquitoes, aedes aegypti (dengue vector) and culex quinquefasciatus (filarial vector). insecticidal susceptibility tests were conducted and the mortality rate was observed after 24 h exposure. the c ... | 0 | 27168688 |
| zika virus: a roar after years of whispering. | 0 | 27168917 | |
| composition and genomic organization of arthropod hox clusters. | the ancestral arthropod is believed to have had a clustered arrangement of ten hox genes. within arthropods, hox gene mutations result in transformation of segment identities. despite the fact that variation in segment number/character was common in the diversification of arthropods, few examples of hox gene gains/losses have been correlated with morphological evolution. furthermore, a full appreciation of the variation in the genomic arrangement of hox genes in extant arthropods has not been re ... | 2016 | 27168931 |
| toxicological evaluation of essential oil from the leaves of croton tetradenius (euphorbiaceae) on aedes aegypti and mus musculus. | for control of aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of croton tetradenius on ae. aegypti larvae and adults. we also evaluated the acute toxicity in mus musculus. the essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. female mice were used for assessing tox ... | 2016 | 27169864 |
| argonaute 2 suppresses japanese encephalitis virus infection in aedes aegypti. | there are three main innate immune mechanisms against viruses in mosquitoes. infection with the flavivirus dengue virus is controlled by rna interference (rnai) and the jak-stat and toll signaling pathways. this study showed that another flavivirus, japanese encephalitis virus (jev), did not invade the salivary glands of aedes aegypti and that this may be a result of the innate immune resistance to the virus. argonaute 2 (ago2) plays a critical role in the rnai pathway. to understand the mechani ... | 2017 | 27169949 |
| mosquito c-type lectins maintain gut microbiome homeostasis. | the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ... | 2016 | 27170846 |
| experimental studies of susceptibility of italian aedes albopictus to zika virus. | we report a study on vector competence of an italian population of aedes albopictus for zika virus (zikv). ae. albopictus was susceptible to zikv infection (infection rate: 10%), and the virus could disseminate and was secreted in the mosquito's saliva (dissemination rate: 29%; transmission rate: 29%) after an extrinsic incubation period of 11 days. the observed vector competence was lower than that of an ae. aegypti colony tested in parallel. | 2016 | 27171034 |
| determinants of arbovirus vertical transmission in mosquitoes. | vertical transmission (vt) and horizontal transmission (ht) of pathogens refer to parental and non-parental chains of host-to-host transmission. combining ht with vt enlarges considerably the range of ecological conditions in which a pathogen can persist, but the factors governing the relative frequency of each transmission mode are poorly understood for pathogens with mixed-mode transmission. elucidating these factors is particularly important for understanding the epidemiology of arthropod-bor ... | 2016 | 27171170 |
| reduced incidence of chikungunya virus infection in communities with ongoing aedes aegypti mosquito trap intervention studies - salinas and guayama, puerto rico, november 2015-february 2016. | aedes species mosquitoes transmit chikungunya virus, as well as dengue and zika viruses, and bite most often during the day.* infectious mosquito bites frequently occur in and around homes (1,2). caribbean countries first reported local transmission of chikungunya virus in december 2013, and soon after, chikungunya virus spread throughout the americas (3). puerto rico reported its first laboratory-positive chikungunya case in may 2014 (4), and subsequently identified approximately 29,000 suspect ... | 2016 | 27171600 |