Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| enterococcus faecium and enterococcus faecalis in blood of newborns with suspected nosocomial infection. | enterococci are gram-positive cocci saprophyte of the human gastrointestinal tract, diners who act as opportunistic pathogens. they can cause infections in patients hospitalized for a long time or who have received multiple antibiotic therapy. enterococcus faecalis and enterococcus faecium are the most common species in human infections. to evaluate the possibility of rapid detection of these species and their occurrence in the blood of newborns with suspected nosocomial infection, blood samples ... | 2017 | 24553613 |
| polymerase chain reaction of enterococcus faecalis and candida albicans in apical periodontitis from turkish patients. | the aim of this study was to determine the frequency of two important pathogenic microorganisms associated with endodontic infections, enterococcus faecalis and candida albicans, in root canal samples from patients with necrotic pulps or failed canal therapy by polymerase chain reaction method. | 2012 | 24558522 |
| chemomechanical preparation by hand instrumentation and by mtwo engine-driven rotary files, an ex vivo study. | to compare the disinfecting efficacy of the sodium hypochlorite irrigation by root canal preparation with stainless steel hand files, taper 0.02 and nickel-titanium mtwo files with taper 0.04-0.06. | 2012 | 24558545 |
| cell-recycle continuous fermentation of enterococcus faecalis rky1 for economical production of lactic acid by reduction of yeast extract supplementation. | both lactic acid productivity and cell growth were linearly correlated with yeast extract supplementation in batch fermentation. during conventional continuous operation, although fresh feed was introduced into the bioreactor with a significantly low dilution rate (0.04 h(-1)), the amount of yeast extract employed was not enough to maintain the growth of microorganism. however, when the fresh feed contained 100 g/l glucose and 2 g/l yeast extract during cell-recycle continuous operation at a dil ... | 2014 | 24561722 |
| is there a need for bacterial endocarditis prophylaxis in patients undergoing urological procedures? | heart valve repair or replacement is a serious problem.the focused update on infective endocarditis of american college of cardiology/american heart association 2008 (acc/aha guidelines) and guidelines on the prevention, diagnosis, and treatment of infective endocarditis (new version 2009) of the european society of cardiology (esc guidelines) describe prophylaxis against infective endocarditis as not recommended for urinary tract procedures in the absence of active infection. a statistical asso ... | 2014 | 24566724 |
| is there a need for bacterial endocarditis prophylaxis in patients undergoing gastrointestinal endoscopy? | heart valve repair or replacement is a serious problem. patients can benefit from an open dialogue between both cardiologists and gastroenterologists for the optimal effective patients care. the focused update on infective endocarditis of the american college of cardiology/american heart association 2008 (acc/aha guidelines) and guidelines on the prevention, diagnosis, and treatment of infective endocarditis (new version 2009) of the european society of cardiology (esc guidelines) describe proph ... | 2014 | 24566725 |
| genome sequencing reveals the environmental origin of enterococci and potential biomarkers for water quality monitoring. | enterococci are common members of the gut microbiome and their ease of culturing has facilitated worldwide use as indicators of fecal pollution of waters. however, enterococci were recently shown to persist in environmental habitats, often in the absence of fecal input, potentially confounding water quality assays. toward resolving this issue and providing a more complete picture of natural enterococci diversity, 11 isolates of enterococcus faecalis recovered from freshwater watersheds (environm ... | 2014 | 24571132 |
| spiromastixones a-o, antibacterial chlorodepsidones from a deep-sea-derived spiromastix sp. fungus. | fifteen new depsidone-based analogues named spiromastixones a-o (1-15) were isolated from the fermentation broth of a deep-sea spiromastix sp. fungus. their structures were elucidated on the basis of extensive nmr and mass spectroscopic analysis in association with chemical conversion. spiromastixones a-o are classified into two subtypes based on the orientation of ring c relative to ring a, while the n-propyl substituents on rings a and c are rarely seen in natural products. most analogues are ... | 2014 | 24571273 |
| daptomycin-loaded polymethylmethacrylate bone cement for joint arthroplasty surgery. | antibiotic-loaded acrylic bone cement has been frequently used as an infection prophylaxis or antibiotic-loaded spacer in infected arthroplasty. in addition, daptomycin has been used recently against broad spectrum gram-positive organisms. the goal of this in vitro study is to investigate the bacteriacidal and mechanical properties of daptomycin-incorporated polymethylmethacrylate (pmma) bone cement and evaluate its feasibility for clinical use. daptomycin (0.5, 1, or 2 g) was premixed with 40 g ... | 2014 | 24571555 |
| microwave-assisted preparation of azachalcones and their n-alkyl derivatives with antimicrobial activities. | two new azachalcones were prepared by both claisen-schmidt condensation and a simple environmentally trendy microwave-assisted method. ten new n-alkyl (c6,8,10,12,14)-substituted azachalconium bromides (3a-e, 4a-e) were prepared from compounds 1 and 2 with corresponding alkyl halides. the antimicrobial activities of all the compounds were tested against enterococcus faecalis, yersinia pseudotuberculosis, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus micro-organisms. | 2014 | 24571646 |
| recombinant human lipocalin 2 acts as an antibacterial agent to prevent platelet contamination. | bacterial contamination of platelet products is the major infectious risk in blood transfusion medicine, which can result in life-threatening sepsis in recipient. lipocalin 2 (lcn2) is an iron-sequestering protein in the antibacterial innate immune response, which inhibit bacterial growth. this study was aimed to evaluate the antibacterial property of lcn2 in preventing bacterial contamination of platelets. | 2014 | 24580532 |
| photodynamic therapy versus ultrasonic irrigation: interaction with endodontic microbial biofilm, an ex vivo study. | photodynamic therapy was introduced as an adjuvant to conventional chemo-mechanical debridement during endodontic treatment to overcome the persistence of biofilms. the aim of this study was to evaluate the ability of photodynamic therapy (pdt) to disrupt an experimental microbial biofilm inside the root canal in a clinically applicable working time. | 2014 | 24583092 |
| effects of single- and multi-strain probiotics on biofilm formation and in vitro adhesion to bladder cells by urinary tract pathogens. | there is increasing evidence that probiotic bacteria can inhibit and/or prevent urinary tract infections. possible mechanisms include prevention of adhesion of pathogens to the bladder epithelium and inhibition of biofilm formation. currently there is interest in the comparative efficacy of single probiotics vs. strain mixtures. we have therefore tested the inhibitory activity of four single probiotics and four probiotic mixtures towards the urinary tract pathogens escherichia coli nctc 9001 and ... | 2014 | 24583095 |
| enterococcus infection biology: lessons from invertebrate host models. | the enterococci are commensals of the gastrointestinal tract of many metazoans, from insects to humans. while they normally do not cause disease in the intestine, they can become pathogenic when they infect sites outside of the gut. recently, the enterococci have become important nosocomial pathogens, with the majority of human enterococcal infections caused by two species, enterococcus faecalis and enterococcus faecium. studies using invertebrate infection models have revealed insights into the ... | 2014 | 24585051 |
| an in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against enterococcus faecalis. | enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic and acquired resistance to a variety of antibiotics. development of new antibiotics is limited, and pathogens continually generate new antibiotic resistance. many researchers aim to identify strategies to effectively kill this drug-resistant pathogen. here, we evaluated the effect of the antimicrobial peptide nisin on the antibacterial activities of 18 antibiotics against e. faecal ... | 2014 | 24586598 |
| effectiveness of ktp laser versus 980 nm diode laser to kill enterococcus faecalis in biofilms developed in experimentally infected root canals. | this study aimed to evaluate the antibacterial action of ktp (potassium-titanyl-phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on enterococcus faecalis biofilms. fifty-six dental roots with single canals were prepared with ni-ti rotary instruments, autoclaved, inoculated with an e. faecalis suspension and incubated for 72 h. they were randomly allocated to control and treatment groups. laser parameters were as follows: power ... | 2015 | 24588799 |
| the antibacterial activity, antioxidant activity and selectivity index of leaf extracts of thirteen south african tree species used in ethnoveterinary medicine to treat helminth infections. | diseases caused by bacteria remain a major challenge globally and particularly in sub-saharan africa. the plants used in this study have been used in south africa to treat helminth infections in livestock and humans. in a previous study we found a correlation between antifungal and anthelmintic activity in some cases. in this study we examined other potential uses of these thirteen plant species by determining the antibacterial and antioxidant activity of the leaf acetone extracts.the antibacter ... | 2014 | 24589020 |
| analysis of the promoters involved in enterocin as-48 expression. | the enterocin as-48 is the best characterized antibacterial circular protein in prokaryotes. it is a hydrophobic and cationic bacteriocin, which is ribosomally synthesized by enterococcal cells and post-translationally cyclized by a head-to-tail peptide bond. the production of and immunity towards as-48 depend upon the coordinated expression of ten genes organized in two operons, as-48abc (where genes encoding enzymes with processing, secretion, and immunity functions are adjacent to the structu ... | 2014 | 24594763 |
| etiological factors of infections in diabetic foot syndrome - attempt to define optimal empirical therapy. | diabetic foot syndrome (dfs) represents one of the most frequent reasons for lower limb amputation in developed countries. in most cases, it is associated with bacterial infection, requiring optimal antibiotic therapy. | 2014 | 24596002 |
| antibacterial substances from marine algae isolated from jeddah coast of red sea, saudi arabia. | marine algae are known to produce a wide variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. however algae of the red sea have not been adequately explored for their potential as a source of bioactive substances. in this context ulva reticulata, caulerpa occidentalis, cladophora socialis, dictyota ciliolata, and gracilaria dendroides isolated from red sea coastal waters of jeddah ... | 2014 | 24596500 |
| comparative evaluation of push-out bond strength of three endodontic sealers with and without amoxicillin-an invitro study. | the purpose of this study was to evaluate the sealing ability of three different root canal sealers with and without amoxicillin, using a push-out bond strength test. | 2014 | 24596782 |
| performance of the eucast disk diffusion method, the clsi agar screen method, and the vitek 2 automated antimicrobial susceptibility testing system for detection of clinical isolates of enterococci with low- and medium-level vanb-type vancomycin resistance: a multicenter study. | different antimicrobial susceptibility testing methods to detect low-level vancomycin resistance in enterococci were evaluated in a scandinavian multicenter study (n=28). a phenotypically and genotypically well-characterized diverse collection of enterococcus faecalis (n=12) and enterococcus faecium (n=18) strains with and without nonsusceptibility to vancomycin was examined blindly in danish (n=5), norwegian (n=13), and swedish (n=10) laboratories using the eucast disk diffusion method (n=28) a ... | 2014 | 24599985 |
| novel benzoxazoles: synthesis and antibacterial, antifungal, and antitubercular activity against antibiotic-resistant and -sensitive microbes. | a new series of 5-(p-substituted benzamido/phenylacetamido)-2-(p-tert-butylphenyl)benzoxazole derivatives were synthesized and evaluated for their antibacterial, antifungal, and antimycobacterial activities against antibiotic-resistant and -sensitive staphylococcus aureus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, and mycobacterium tuberculosis as well as against candida albicans and candida krusei. the compounds possessed broad-spectrum activity against all of the tested g ... | 2014 | 24601083 |
| antibacterial properties and mode of action of new triaryl butene citrate compounds. | the aim of this study was to evaluate the antibacterial activity of newly synthesized triaryl butene analogues of tamoxifen. several compounds were synthesized and converted to citrate salts to ensure greater solubility. four compounds showed significant antibacterial activity at micromolar concentrations against gram-positive and gram-negative foodborne pathogens including listeria monocytogenes, listeria ivanovii, enterococcus faecalis, staphylococcus aureus and escherichia coli. two compounds ... | 2014 | 24602786 |
| effects of intracanal irrigant mtad combined with nisin at sub-minimum inhibitory concentration levels on enterococcus faecalis growth and the expression of pathogenic genes. | exposure to antibiotics is considered to be the major driver in the selection of antibiotic-resistant bacteria and may induce diverse biological responses in bacteria. mtad is a common intracanal irrigant, but its bactericidal activity remains to be improved. previous studies have indicated that the antimicrobial peptide nisin can significantly improve the bactericidal activity of mtad against enterococcus faecalis. however, the effects of mtad and its modification at sub-minimum inhibitory conc ... | 2014 | 24603760 |
| significance and survival of enterococci during the house fly development. | house flies are among the most important nonbiting insect pests of medical and veterinary importance. larvae develop in decaying organic substrates and their survival strictly depends on an active microbial community. house flies have been implicated in the ecology and transmission of enterococci, including multi-antibiotic-resistant and virulent strains of enterococcus faecalis. in this study, eight american type culture collection type strains of enterococci including enterococcus avium, enter ... | 2014 | 24605454 |
| [development of polycomponent metabolite probiotic]. | construct composition of polycomponent probiotic based on lactobacilli cultural fluid (cf) supernatant. | 2014 | 24605659 |
| endoe from enterococcus faecalis hydrolyzes the glycans of the biofilm inhibiting protein lactoferrin and mediates growth. | glycosidases are widespread among bacteria. the opportunistic human pathogen enterococcus faecalis encodes several putative glycosidases but little is known about their functions. the identified endo-β-n-acetylglucosaminidase endoe has activity on the n-linked glycans of the human immunoglobulin g (igg). in this report we identified the human glycoprotein lactoferrin (hlf) as a new substrate for endoe. hydrolysis of the n-glycans from hlf was investigated using lectin blot, uhplc and mass spectr ... | 2014 | 24608122 |
| antibacterial, anti-inflammatory and probiotic potential of enterococcus hirae isolated from the rumen of bos primigenius. | in the present study bacterial strains were isolated from the rumen fluids of bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens vibrio cholera, enterococcus faecalis, enterobacter aerogens, pseudomonas ... | 2014 | 24609495 |
| linezolid resistance in vancomycin-resistant enterococcus faecalis and enterococcus faecium isolates in a brazilian hospital. | 2014 | 24614366 | |
| spread of multidrug-resistant enterococcus faecalis within the household setting. | advances in veterinary medicine have resulted in the survival of many animals with severe illness or infectious diseases. in addition, increased usage of antimicrobial agents for veterinary purposes has contributed to the worldwide problem of increasing antimicrobial resistance. the objective of this study was to contribute to better understand the potential and implications for the spread of antimicrobial-resistant enterococci between pets receiving antimicrobial treatments and their owners. th ... | 2014 | 24617521 |
| inactivation of airborne enterococcus faecalis and infectious bursal disease virus using a pilot-scale ultraviolet photocatalytic oxidation scrubber. | high microbial concentrations and emissions associated with livestock houses raise health and environmental concerns. a pilot-scale ultraviolet photocatalytic oxidation (uv-pco) scrubber was tested for its efficacy to inactivate aerosolized enterococcus faecalis and infectious bursal disease virus (ibdv). microbial reduction was determined by the difference in microbial concentrations measured in the upstream and downstream isolators that were connected to the two ends of the uv-pco scrubber. tw ... | 2014 | 24620401 |
| the skpo-1 peroxidase functions in the hypodermis to protect caenorhabditis elegans from bacterial infection. | in recent years, the synergistic relationship between nadph oxidase (nox)/dual oxidase (duox) enzymes and peroxidases has received increased attention. peroxidases utilize nox/duox-generated h2o2 for a myriad of functions including, but not limited to, thyroid hormone biosynthesis, cross-linking extracellular matrices (ecm), and immune defense. we postulated that one or more peroxidases produced by caenorhabditis elegans would act in host defense, possibly in conjunction with bli-3, the only nox ... | 2014 | 24621828 |
| antibacterial effect of iranian green-tea-containing mouthrinse vs chlorhexidine 0.2%: an in vitro study. | considering the antioxidant, anti-inflammatory and antimicrobial properties of green tea, this study aimed to evaluate the antibacterial effect of mouthrinses containing green tea extract vs 0.2% chlorhexidine on selected microorganisms in vitro. | 2014 | 24624389 |
| evaluation of cytotoxicity, antimicrobial activity and physicochemical properties of a calcium aluminate-based endodontic material. | a calcium aluminate-based endodontic material, endobinder, has been developed in order to reduce mta negative characteristics, preserving its biological properties and clinical applications. | 2015 | 24626250 |
| bacteriocinogenic properties and in vitro probiotic potential of enterococci from tunisian dairy products. | the aim of this study was to isolate new bacteriocinogenic strains with putative probiotic potential from various tunisian fermented milks. a total of 44 gram-positive catalase-negative isolates were colony-purified and screened for antimicrobial activity. of inhibitory isolates, four were identified as enterococcus durans and one as enterococcus faecalis using 16s rrna gene sequence. the five strains were sensitive to penicillin g, all aminoglycosides tested, to the vancomycin, tetracycline, an ... | 2014 | 24627155 |
| antibacterial efficacy testing of a bioelectric wound dressing against clinical wound pathogens. | silver-based wound dressings have been developed for the control of bioburden in wounds. however, the popularity and extensive use of silver-based dressings has been associated with emerging microbial resistances to silver. in this study we examined in vitro antibacterial efficacy of a bioelectric dressing containing silver and zinc against various wound pathogens. antibiotic-sensitive clinical wound isolates showed a 100% reduction in bacterial growth, except that enterococcus faecalis isolate ... | 2014 | 24627730 |
| drug-resistant and hospital-associated enterococcus faecium from wastewater, riverine estuary and anthropogenically impacted marine catchment basin. | enterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (hais). it is believed that the recent evolution of two clinically relevant species, enterococcus faecalis and enterococcus faecium occurred in a big part in a hospital environment, leading to formation of high-risk enterococcal clonal complexes (hireccs), which combine multidrug resistance with increased pathogenicity and epidemicity. the aim of this study was ... | 2014 | 24629030 |
| green synthesis of silver nanoparticles using alternanthera dentata leaf extract at room temperature and their antimicrobial activity. | a green rapid biogenic synthesis of silver nanoparticles agnps using alternanthera dentata (a. dentata) aqueous extract was demonstrated in this present study. the formation of silver nanoparticles was confirmed by surface plasmon resonance (spr) at 430nm using uv-visible spectrophotometer. the reduction of silver ions to silver nanoparticles by a. dentata extract was completed within 10min. synthesized nanoparticles were characterized using uv-visible spectroscopy; fourier transformed infrared ... | 2014 | 24632169 |
| daptomycin activity tested against 164457 bacterial isolates from hospitalised patients: summary of 8 years of a worldwide surveillance programme (2005-2012). | we report the results of 8 years (2005-2012) of the daptomycin surveillance programme worldwide. consecutive non-duplicate bacterial isolates (prevalence design) were collected from patients with documented infections in 410 medical centres and were susceptibility tested by reference broth microdilution methods. a total of 164457 gram-positive isolates were evaluated, including 97542 staphylococcus aureus, 21413 coagulase-negative staphylococci (cons), 29619 enterococci and 15883 β-haemolytic st ... | 2014 | 24636430 |
| impact of staphylococcus aureus on pathogenesis in polymicrobial infections. | polymicrobial infections involving staphylococcus aureus exhibit enhanced disease severity and morbidity. we reviewed the nature of polymicrobial interactions between s. aureus and other bacterial, fungal, and viral cocolonizers. microbes that were frequently recovered from the infection site with s. aureus are haemophilus influenzae, enterococcus faecalis, pseudomonas aeruginosa, streptococcus pneumoniae, corynebacterium sp., lactobacillus sp., candida albicans, and influenza virus. detailed an ... | 2014 | 24643542 |
| characterization of aid1, a novel gene involved in fusobacterium nucleatum interspecies interactions. | the oral opportunistic pathogen fusobacterium nucleatum is known to interact with a large number of different bacterial species residing in the oral cavity. it adheres to a variety of gram-positive bacteria, including oral streptococci via the arginine-inhibitable adhesin radd. in this study, we describe a novel protein encoded by the predicted open reading frame fn1253 that appears to play a role in interspecies interactions of f. nucleatum, particularly with oral streptococci and related gram- ... | 2014 | 24643713 |
| systematic evaluation of commercial susceptibility testing methods for determining the in vitro activity of daptomycin versus staphylococcus aureus and enterococci. | we systematically evaluated 5 methods for testing daptomycin versus 48 enterococcus faecalis, 51 enterococcus faecium, and 50 staphylococcus aureus isolates using (i and ii) broth microdilution (bmd) with 50-mg/liter calcium medium supplementation (reference method) and 30-mg/liter calcium medium supplementation (bmd30 method), (iii) etest, and (iv and v) microscan panel 33 using 2 methods to prepare the bacterial inoculum (microscan turbidity and microscan prompt). isolates were categorized as ... | 2014 | 24648560 |
| [in vitro activity of sitafloxacin against clinical isolates in 2012]. | in vitro activity of sitafloxacin (stfx) and various oral antimicrobial agents against bacterial isolates recovered from clinical specimens between january and december 2012, at different healthcare facilities in japan was evaluated. a total of 1,620 isolates including aerobic and anaerobic organisms were available for the susceptibility testing using the microbroth dilution methods recommended by clinical and laboratory standards institute. the minimum inhibitory concentration of stfx at which ... | 2013 | 24649797 |
| bacterial isolates associated with pelvic inflammatory disease among female patients attending some hospitals in abuja, nigeria. | pelvic inflammatory disease refers to any infection in the female lower reproductive tract that spreads to the upper reproductive tract. the disease comprises a spectrum of inflammatory disorders of the upper female genital tract, including any combination of endometritis, salpingitis, tubo-ovarian abscess and pelvic peritonitis. pid is not a notifiable disease in most countries, so accurate statistics are not available. this situation is not in any way different here in nigeria and more so in t ... | 2014 | 24653811 |
| is the mazef toxin-antitoxin system responsible for vancomycin resistance in clinical isolates of enterococcus faecalis? | the current study was conducted to investigate the relationship between vancomycin-resistant enterococcus faecalis (vre) and the presence of mazef toxin-antitoxin (ta) system, which may be useful as target for novel antimicrobial therapy concepts. the susceptibility of e. faecalis was determined by mic, and the presence of the mazef ta system was evaluated by pcr. among 200 e. faecalis isolates 39.5% showed resistance to vancomycin (vre), while 60.5% were susceptible strains (vse). the mazef ta ... | 2014 | 24653969 |
| in vitro activity of photoactivated disinfection using a diode laser in infected root canals. | to investigate the lethal activity of photoactivated disinfection (pad) on enterococcus faecalis (atcc 29212) and mixed populations of aerobic or anaerobic bacteria in infected root canals using a diode laser after the application of a photosensitizer (ps). | 2014 | 24655313 |
| aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity. | liamocins are structurally unique, heavier-than-water “oils” produced by certain strains of aureobasidium pullulans. the aim of the current study is to identify new sources of liamocins and evaluate their potential as anticancer agents. nine strains of a. pullulans from phylogenetic clades 8, 9, and 11 were examined for the first time for production of liamocins. strains in these clades have only been isolated from tropical environments, and all strains tested here were from various locations in ... | 2014 | 24659335 |
| global metabolic response of enterococcus faecalis to oxygen. | oxygen and oxidative stress have become relevant components in clarifying the mechanism that weakens bacterial cells in parallel to the mode of action of bactericidal antibiotics. given the importance of oxidative stress in the overall defense mechanism of bacteria and their apparent role in the antimicrobial mode of action, it is important to understand how bacteria respond to this stress at a metabolic level. the aim of this study was to determine the impact of oxygen on the metabolism of the ... | 2014 | 24659768 |
| synergistic antibacterial effect of honey and herba ocimi basilici against some bacterial pathogens. | to evaluate the antibacterial activity of the combination of different honey brands and methanolic fraction of herba ocimi basilici using agar well diffusion assay. | 2013 | 24660616 |
| antibacterial properties of water-soluble gold(i) n-heterocyclic carbene complexes. | the antibacterial properties of water-soluble gold(i) complexes [1-methyl-3-(3-sulfonatopropyl)imidazol-2-ylidene]gold(i) chloride (c1), [1-mesityl-3-(3-sulfonatopropyl)imidazol-2-ylidene]gold(i) chloride (c2), [1-(2,6-diisopropylphenyl)-3-(3-sulfonatopropyl)imidazol-2-ylidene]gold(i) chloride (c3) and [1,3-bis(2,6-diisopropyl-4-sodiumsulfonatophenyl)imidazol-2-ylidene]gold(i) chloride (c4) and the respective ligands were assessed by agar diffusion and broth macrodilution methods against gram-po ... | 2014 | 24662463 |
| toxicity of magnetic chitosan micro and nanoparticles as carriers for biologically active substances. | nanoparticles of inorganic magnetic core surrounded by layers of functional coatings are potential representatives of nanostructures for immobilization of bio-substances. magnetic nanoparticles (mnps) are often bound in aggregates due to a strong magnetic dipole, which has a lot of advantages, such as large surface area for binding biologically active substances. chitosan is a polysaccharide polymer that is non-toxic, hydrophilic, biocompatible and has hydroxy and amino groups in its structure. ... | 2014 | 24664338 |
| quorum sensing in gram-positive bacteria: assay protocols for staphylococcal agr and enterococcal fsr systems. | a thiolactone/lactone peptide-mediated quorum sensing (qs) system is commonly employed in gram-positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. here, we describe assay protocols for the well-studied qs systems (agr and fsr) of two representative gram-positive pathogens, staphylococcus aureus and enterococcus faecalis. these convenient assay systems are useful for the screening of qs inhibitors as well as ... | 2014 | 24664824 |
| dentin extends the antibacterial effect of endodontic sealers against enterococcus faecalis biofilms. | the purpose of this study was to evaluate the antimicrobial effects of root canal sealers on enterococcus faecalis biofilms in dentinal tubules by using a novel dentin infection model. | 2014 | 24666900 |
| effect of long-term exposure to endodontic disinfecting solutions on young and old enterococcus faecalis biofilms in dentin canals. | the purpose of this study was to evaluate the antimicrobial activity on enterococcus faecalis biofilms in dentin canals of short-term and long-term exposure to different endodontic disinfecting solutions by using a dentin infection model and confocal laser scanning microscopy. | 2014 | 24666901 |
| antibacterial efficacy of photosensitizer functionalized biopolymeric nanoparticles in the presence of tissue inhibitors in root canal. | application of antibacterial nanoparticles to improve root canal disinfection has received strong interest recently. the current study aims to assess the antibacterial effect of a novel photosensitizer (rose bengal functionalized chitosan nanoparticles [csrbnp]) to eliminate bacteria in the presence of various root canal constituents that are known to inhibit the antibacterial efficacy of root canal disinfectants. | 2014 | 24666913 |
| application of nanometal oxides in situ in nonwoven polyester fabric for the removal of bacterial indicators of pollution from wastewater. | the objective of this study is to investigate and assess the use of in situ deposit nanosilver (nag2o) or nanocopper oxides (ncuo) into nonwoven polyester fabric (nwpf) as a safe and effective antibacterial filter of pollution from domestic wastewater. the bactericidal effect of both nag2o and ncuo was examined against gram-negative bacteria (escherichia coli, salmonella typhi) and gram-positive bacteria (enterococcus faecalis, staphylococcus aureus) using agar diffusion disk method. in addition ... | 2014 | 24672400 |
| insusceptibility to disinfectants in bacteria from animals, food and humans-is there a link to antimicrobial resistance? | enterococcus faecalis (n = 834) and enterococcus faecium (n = 135) from blood and feces of hospitalized humans, from feces of outpatients and livestock and from food were screened for their susceptibility to a quaternary ammonium compound (didecyldimethylammoniumchloride, ddac) and to 28 antibiotics by micro-/macrodilution. the maximum ddac-mic in our field study was 3.5 mg/l, but after adaptation in the laboratory, mic values of 21.9 mg/l were observed. strains for which ddac had mics > 1.4 mg/ ... | 2014 | 24672513 |
| draft genome sequence of a new homofermentative, lactic acid-producing enterococcus faecalis isolate, cbrd01. | we report here the draft genome sequence of the novel homofermentative enterococcus faecalis isolate cbrd01, which is capable of high lactic acid productivity and yields, with minimal nutritional requirements. the genome is 2.8 mbp, with 37% g+c, and contains genes for two lactate dehydrogenase (ldh) enzymes found in related organisms. | 2014 | 24675849 |
| bacillus subtilis as a platform for molecular characterisation of regulatory mechanisms of enterococcus faecalis resistance against cell wall antibiotics. | to combat antibiotic resistance of enterococcus faecalis, a better understanding of the molecular mechanisms, particularly of antibiotic detection, signal transduction and gene regulation is needed. because molecular studies in this bacterium can be challenging, we aimed at exploiting the genetically highly tractable gram-positive model organism bacillus subtilis as a heterologous host. two fundamentally different regulators of e. faecalis resistance against cell wall antibiotics, the bacitracin ... | 2014 | 24676422 |
| in vitro comparison of the effects of probiotic, commensal and pathogenic strains on macrophage polarization. | macrophages are important with respect to both innate and adaptive immune responses and are known to differentiate into pro-inflammatory m1- or anti-inflammatory m2-phenotypes following activation. in order to study how different bacteria affect macrophage polarization, we exposed murine raw 264.7 macrophages to sixteen different strains representing probiotic strains, pathogens, commensals and strains of food origin. increased inducible nitric oxide synthase (inos) or arginase-1 gene expression ... | 2014 | 24676762 |
| a review of the current place of glycopeptides in turkish medical practice. | glycopeptide antibiotics are considered by many investigators to be the last resort in the treatment of gram-positive bacterial infections. | 2007 | 24678118 |
| identification of temporal clusters and risk factors of bacteremia by nosocomial vancomycin-resistant enterococci. | this study aimed to evaluate a different methodology for addressing the evolution of nosocomial bacteremia by vancomycin-resistant enterococci (vre) in a hospital setting. | 2014 | 24679566 |
| influence of in vitro gastrointestinal digestion of fruit juices enriched with pine bark extract on intestinal microflora. | the selective antimicrobial effect of fruit juices enriched with pine bark extract (pbe) (0.5 g/l) has been studied before and after in vitro gastrointestinal digestion. pbe (a concentrate of water-soluble bioflavonoids, mainly including phenolic compounds) has been proven to have high stability to the digestion process. pure phenolic compounds such as gallic acid had a high antimicrobial effect on staphylococcus aureus and escherichia coli, maintaining the lactic acid bacteria population (≈100% ... | 2014 | 24679746 |
| heterologous expression and characterization of tyrosine decarboxylase from enterococcus faecalis r612z1 and enterococcus faecium r615z1. | tyrosine decarboxylase (tdc) is responsible for tyramine production and can catalyze phenylalanine to produce β-phenylethylamine. enterococcus strains are a group of bacteria predominantly producing tyramine and β-phenylethylamine in water-boiled salted duck. in this study, the heterologous expression and characterization of two tdcs from enterococcus faecalis r612z1 (612tdc) and enterococcus faecium r615z1 (615tdc) were studied. the recombinant putative proteins of 612tdc and 615tdc were hetero ... | 2014 | 24680070 |
| protection of mice from oral candidiasis by heat-killed enterococcus faecalis, possibly through its direct binding to candida albicans. | to develop a new therapy against oral candidiasis, a commensal microorganism, enterococcus faecalis was tested for its ability to modulate candida growth in vitro and its therapeutic activities against a murine model in vivo. addition of heat-killed e. faecalis strain ef2001 (ef2001) isolated from healthy human feces to the culture of c. albicans strain timm1768 inhibited adherence of the latter to a microtiter plate in a dose dependent manner and candida cells surrounded by ef2001 were increase ... | 2014 | 24682096 |
| antimicrobial effect of lippia sidoides and thymol on enterococcus faecalis biofilm of the bacterium isolated from root canals. | the species lippia sidoides cham. (verbenaceae) is utilized in popular medicine as a local antiseptic on the skin and mucosal tissues. enterococcus faecalis is the bacterium isolated from root canals of teeth with persistent periapical lesions and has the ability to form biofilm, where it is responsible for the failure of endodontic treatments. essential oil of l. sidoides (eols) and its major component, thymol, were evaluated for reducing the cfu in biofilms of e. faecalis in vitro. the essenti ... | 2014 | 24683344 |
| an in vitro comparison of antimicrobial effcacy of three root canal irrigants-biopure mtad, 2% chlorhexidine gluconate and 5.25% sodium hypochlorite as a final rinse against e. faecalis. | this study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (naocl), 2% chlorhexidine (chx) and biopure mtad when used as a fnal rinse against enterococcus faecalis. | 2013 | 24685785 |
| characterization of sal(a), a novel gene responsible for lincosamide and streptogramin a resistance in staphylococcus sciuri. | natural resistance to lincosamides and streptogramins a (lsa), which is a species characteristic of bacillus subtilis and enterococcus faecalis, has never been documented in the staphylococcus genus. we investigate here the molecular basis of the lsa phenotype exhibited by seven reference strains of staphylococcus sciuri, including the type strains of the three described subspecies. by whole-genome sequencing of strain atcc 29059, we identified a candidate gene that encodes an atp-binding casset ... | 2014 | 24687494 |
| the ability of triple antibiotic paste and calcium hydroxide in disinfection of dentinal tubules. | the purpose of this in vitro study was to compare the ability of triple antibiotic paste (tap) to calcium hydroxide (ch) in disinfecting dentinal tubules. | 2014 | 24688581 |
| methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus faecalis and enterococcus faecium active dimeric isobutyrylphloroglucinol from ivesia gordonii. | bioassay-guided fractionation of the chloroform soluble fraction of stem, leaf, and flower extracts of the american plant ivesia gordonii led to the isolation of a new dimeric acylphloroglucinol, 3,3'-diisobutyryl-2,6'-dimethoxy-4,6,2',4'-tetrahydroxy-5,5'dimethyldiphenyl methane (1), to which we have assigned the trivial name ofivesinol (1), together with a known monomeric acylphloroglucinol, 1,5-dihydroxy-2-(2'-methylpropionyl)-3-methoxy-6-methylbenzene (2). the structures of the isolated comp ... | 2014 | 24689296 |
| antimicrobial prenylated benzoylphloroglucinol derivatives and xanthones from the leaves of garcinia goudotiana. | bioassay-guided fractionation using antimicrobial assay of the crude acetonic extract of garcinia goudotiana leaves and of its five partitions led to the isolation of two new prenylated benzoylphloroglucinol derivatives, goudotianone 1 (1) and goudotianone 2 (2), in addition to two known compounds including one xanthone, 1,3,7-trihydroxy-2-isoprenylxanthone (3), and one triterpenoid, friedelin (4). their structures were elucidated on the basis of different spectroscopic methods, including extens ... | 2014 | 24690454 |
| high inhibition of paenibacillus larvae and listeria monocytogenes by enterococcus isolated from different sources in tunisia and identification of their bacteriocin genes. | a total of 300 isolates of enterococcus, from different sources including faeces of poultry, cow and sheep, raw milk, ricotta cheese and water, in tunisia, were screened for their antibacterial activity. amongst them, 59 bacteriocin-producing strains were detected and identified by molecular methods. genes encoding for enta, entp, entb, entl50a/b, as-48 and bac31 bacteriocins were targeted by pcr. the bacteriocin-producing strains were assigned to the species enterococcus faecium, enterococcus f ... | 2014 | 24698417 |
| transcriptome analysis of enterococcus faecalis toward its adaption to surviving in the mouse intestinal tract. | we have performed a transcriptomic in vivo study with enterococcus faecalis og1rf in the intestine of living mice to identify novel latent and adaptive fitness determinants within e. faecalis. from 2,658 genes that are present in e. faecalis strain og1rf, 124 genes were identified as significantly differentially expressed within the intestinal tract of living mice as compared to exponential growth in bhi broth. the groups of significantly up- or down-regulated genes consisted of 94 and 30 genes, ... | 2014 | 24700373 |
| hospital acquired infections among patients admitted in the medical and surgical wards of a non-teaching secondary care hospital in northern india. | to investigate the incidence of nosocomial infection (ni) and type of bacteriological isolates among the patients admitted in the medical and surgical wards of a non-teaching secondary care hospital in north india. | 2014 | 24701489 |
| cytotoxicity, interaction with dentine and efficacy on multispecies biofilms of a modified salt solution intended for endodontic disinfection in a new in vitro biofilm model. | to investigate the cytotoxicity of a modified salt solution (mss) and evaluate the antimicrobial properties of mss on in vitro biofilm models. | 2015 | 24702260 |
| colonization of enterococcus faecalis in a new sio/sio(2)-microtube in vitro model system as a function of tubule diameter. | endodontic pathogens can penetrate deep into dentinal tubules and therefore survive the chemo-mechanical disinfection procedures. bacterial penetration has been mainly studies using sliced infected human teeth which, besides creating artifacts, can hinder the observation of the inner tubules due to the dense and opaque dentin structure. the aim of the present study was to develop a standardized dentin model by using artificial sio/sio2-microtubes of different diameters and lengths to test the pe ... | 2014 | 24703548 |
| impact of bacterial infections on aging and cancer: impairment of dna repair and mitochondrial function of host cells. | the commensal floras that inhabit the gastrointestinal tract play critical roles in immune responses, energy metabolism, and even cancer prevention. pathogenic and out of place commensal bacteria, can however have detrimental effects on the host, by introducing genomic instability and mitochondrial dysfunction, which are hallmarks of both aging and cancer. helicobacter pylori and enterococcus faecalis are bacteria of the gastrointestinal tract that have been demonstrated to affect these two hall ... | 2014 | 24704713 |
| enhanced removal of enterococcus faecalis biofilms in the root canal using sodium hypochlorite plus photon-induced photoacoustic streaming: an in vitro study. | the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) using er:yag laser energy in decontaminating heavily colonized root canal systems in vitro. | 2014 | 24717113 |
| structural elucidation of srta enzyme in enterococcus faecalis: an emphasis on screening of potential inhibitors against the biofilm formation. | enterococcus faecalis is a pathogenic gram-positive bacterium, which mainly infects humans through urinary tract infections. srta is an essential enzyme for survival of e. faecalis, and inhibition of this particular enzyme will reduce the virulence of biofilm formation. it is proved to be associated with the microbial surface protein embedded signal transduction mechanism and promising as a suitable anti-microbial drug target for e. faecalis. the present work gives an inclusive description of sr ... | 2014 | 24718729 |
| effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity. | recent studies have investigated the anti-obesity effect of resveratrol, but the pathways through which resveratrol resists obesity are not clear. in the present study, we hypothesize that resveratrol exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes, and in turn, improving fat storage and metabolism. gut microbes, glucose and lipid metabolism in high-fat diet (hf) mice in vivo are investigated after resveratrol treatment. several biochemical markers a ... | 2014 | 24722352 |
| occurrence of enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach. | median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<0.0001) with increasing proximity to river influx. the difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. virulence genes cyl, esp, gele and asa were detected at varying frequencies (7.32 ... | 2014 | 24725825 |
| an in vitro comparison of furcal perforation repaired with pro-root mta and new endodontic cement in primary molar teeth- a microleakage study. | one of the most challenging procedural accidents during pulpotomy of primary molars is furcal perforation. to prevent bacterial invasion, the perforation site should be sealed as soon as possible. | 2014 | 24738087 |
| comparison of the antibacterial effect of sodium hypochlorite and aloe vera solutions as root canal irrigants in human extracted teeth contaminated with enterococcus faecalis. | the main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. sodium hypochlorite has excellent antibacterial properties, but also some negative features. | 2014 | 24738089 |
| passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against enterococcus faecalis. | chemomechanical procedures can be used to eliminate bacteria from root canals. however, detectable bacteria sometimes remain because of the complexity of the root canal system. endodontic passive ultrasonic irrigation (pui) with hydrogen peroxide (h2o2) may be a promising option for increasing bactericidal hydroxyl radical (ho•) generation. in this in vitro experiment, we examined the effects of ho• generated using pui and a low concentration of h2o2. an ultrasonic tip was submerged in 0.45 mol/ ... | 2014 | 24739706 |
| glycopeptide use is associated with increased mortality in enterococcus faecalis bacteraemia. | enterococci are an important cause of nosocomial and community-acquired infections, with bacteraemia being one of the most common presentations. although inappropriate antimicrobial therapy has been associated with poorer outcomes in enterococcus faecalis (ef) bacteraemia, the impact of antimicrobial choice, namely β-lactam versus glycopeptide therapy, has not been well described. we sought to determine whether choice of antibiotic affects patient outcomes in ef bacteraemia. | 2014 | 24744303 |
| in vitro efficacy of antibiotic beads in treating abdominal vascular graft infections. | abdominal aortic vascular graft infection often involves several different organisms. antibiotic polymethyl methacrylate (pmma) beads may be effective in controlling infection after débridement, but bacterial species identification and antibiotic susceptibility are often not available at the time of operation, generating a need for a broad-spectrum drug combination for empirical use. we sought to determine an effective antibiotic in pmma beads for use in abdominal vascular graft infection. | 2015 | 24745942 |
| in vitro assessment of antimicrobial activity of pothomorphe umbellata extracts against enterococcus faecalis. | due to the complex anatomy of the root canal system, biomechanical preparation is not able to completely eliminate all microorganisms present in the endodontic infections, making it necessary the use of an intracanal medication. | 2014 | 24748302 |
| clonal diversity in biofilm formation by enterococcus faecalis in response to environmental stress associated with endodontic irrigants and medicaments. | to determine whether clonal diversity within e. faecalis affects biofilm formation when exposed to antimicrobial compounds found in endodontic medicaments and irrigants. | 2015 | 24749689 |
| prevalence and antibiotic resistance of enterococcus spp. isolated from retail cheese, ready-to-eat salads, ham, and raw meat. | food specimens were analyzed in order to research enterococcus spp.: 636 samples of raw meat (227 beef, 238 poultry, and 171 pork), 278 samples of cheese (110 fresh soft cheese and 168 mozzarella cheese), 214 samples of ready-to-eat salads, and 187 samples of ham. 312 strains of enterococcus spp samples were isolated, then identified and submitted to susceptibility tests against 11 antimicrobial agents. the predominant species were enterococcus faecalis in raw meat and enterococcus faecium in re ... | 2014 | 24750807 |
| exploring 5-nitrofuran derivatives against nosocomial pathogens: synthesis, antimicrobial activity and chemometric analysis. | the burden of nosocomial or health care-associated infection (hcai) is increasing worldwide. according to the world health organization (who), it is several fold higher in low- and middle-income countries. considering the multidrug-resistant infections, the development of new and more effective drugs is crucial. herein, two series (i and ii) of 5-nitrofuran derivatives were designed, synthesized and assayed against microorganisms, including gram-positive and -negative bacteria, and fungi. the pa ... | 2014 | 24751553 |
| amikacin-fosfomycin at a five-to-two ratio: characterization of mutation rates in microbial strains causing ventilator-associated pneumonia and interactions with commonly used antibiotics. | the amikacin-fosfomycin inhalation system (afis), a combination of antibiotics administered with an in-line nebulizer delivery system, is being developed for adjunctive treatment of ventilator-associated pneumonia (vap). the in vitro characterization of amikacin-fosfomycin (at a 5:2 ratio) described here included determining resistance selection rates for pathogens that are representative of those commonly associated with vap (including multidrug-resistant strains) and evaluating interactions wi ... | 2014 | 24752276 |
| antimicrobial and immunomodulatory activities of pr-39 derived peptides. | the porcine cathelicidin pr-39 is a host defence peptide that plays a pivotal role in the innate immune defence of the pig against infections. besides direct antimicrobial activity, it is involved in immunomodulation, wound healing and several other biological processes. in this study, the antimicrobial- and immunomodulatory activity of pr-39, and n- and c-terminal derivatives of pr-39 were tested. pr-39 exhibited an unexpected broad antimicrobial spectrum including several gram positive strains ... | 2014 | 24755622 |
| transcriptomic and functional analysis of nacl-induced stress in enterococcus faecalis. | the robust physiology of enterococcus faecalis facilitates tolerance to various stresses. we here report the transcriptional response of e. faecalis v583 to growth in the presence of 6.5% nacl. among the early responses observed was an immediate down-regulation of mscl, accompanied by an up-regulation of genes predicted to be involved in uptake of extracellular potassium and glycine betaine. the high nacl concentration also induced expression of chaperons and cell envelope related traits, such a ... | 2014 | 24755907 |
| evaluation of antimicrobial activity of silver nanoparticles for carboxymethylcellulose film applications in food packaging. | in this study, silver nanoparticles were prepared and incorporated into carboxymethylcellulose films to evaluate the antimicrobial activity for food packaging applications. the techniques carried out for material characterization were: infrared spectroscopy and thermal analysis for the silver nanoparticles and films, as well as particle size distribution for the nanoparticles and water vapor permeability for the films. the antimicrobial activity of silver nanoparticles prepared by casting method ... | 2014 | 24758059 |
| new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. | the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings. | 2014 | 24767562 |
| pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the enterococcus faecalis strain w11. | although anaerobic glycerol metabolism in enterococcus faecalis requires exogenous fumarate for nadh oxidation, e. faecalis strain w11 can metabolize glycerol in the absence of oxygen without exogenous fumarate. in this study, metabolic end product analyses and reporter assays probing the expression of enzymes involved in pyruvate metabolism were performed to investigate this fumarate-independent anaerobic metabolism of glycerol in w11. under aerobic conditions, the metabolic end products of w11 ... | 2014 | 24769696 |
| a newly isolated probiotic enterococcus faecalis strain from vagina microbiota enhances apoptosis of human cancer cells. | this study aimed to describe probiotic properties and bio-therapeutic effects of newly isolated enterococcus faecalis from the human vaginal tract. | 2014 | 24775273 |
| ceftobiprole activity against over 60,000 clinical bacterial pathogens isolated in europe, turkey, and israel from 2005 to 2010. | ceftobiprole medocaril is a newly approved drug in europe for the treatment of hospital-acquired pneumonia (hap) (excluding patients with ventilator-associated pneumonia but including ventilated hap patients) and community-acquired pneumonia in adults. the aim of this study was to evaluate the in vitro antimicrobial activity of ceftobiprole against prevalent gram-positive and -negative pathogens isolated in europe, turkey, and israel during 2005 through 2010. a total of 60,084 consecutive, nondu ... | 2014 | 24777091 |
| evaluation of the novel combination of daptomycin plus ceftriaxone against vancomycin-resistant enterococci in an in vitro pharmacokinetic/pharmacodynamic simulated endocardial vegetation model. | daptomycin has demonstrated synergy with β-lactams against enterococcus faecium and this combination has been used successfully to treat infections refractory to daptomycin. we investigated daptomycin alone and combined with ceftriaxone against vancomycin-resistant enterococci (vre) in an in vitro pharmacokinetic/pharmacodynamic simulated endocardial vegetation (sev) model. | 2014 | 24777900 |