Publications

the response of claudin-like transmembrane septate junction proteins to altered environmental ion levels in the larval mosquito aedes aegypti.	septate junctions (sjs) occlude the paracellular pathway and function as paracellular diffusion barriers within invertebrate epithelia. however, integral components of sjs and their contribution to barrier properties have received considerably less attention than those of vertebrate occluding junctions. in arthropods, sj proteins have only been identified in drosophila and among these are three integral claudin-like proteins, megatrachea (mega), sinuous (sinu) and kune-kune (kune), as well as a ...	2016	27004691
larvicidal and pupicidal activities of alizarin isolated from roots of rubia cordifolia against culex quinquefasciatus say and aedes aegypti (l.) (diptera: culicidae).	the mosquitocidal activities of different fractions and a compound alizarin from the methanol extract of rubia cordifolia roots were evaluated on larvae and pupae of culex quinquefasciatus say and aedes aegypti (l.) (diptera: culicidae). larvae and pupae were exposed to concentrations of 2.5, 5.0, 7.5 and 10 ppm for fractions and 0.5, 1.0, 1.5 and 2.0 ppm for compound. after 24 h, the mortality was assessed and the lc50 and lc90 values were estimated for larvae and pupae. among the 23 fractions ...	2016	27004695
zika virus: yet another emerging threat to nepal.	zika virus (zikv) is a flavivirus with single stranded rna related to yellow fever, dengue, west nile, and japanese encephalitis viruses and is transmitted by aedes mosquitoes primarily by aedes aegipti which is widely distributed in nepal. zikv was first identified incidentally in rhesus monkey in uganda in 1947 and human infection in 1952; and by now outbreaks of zikv disease have been recorded in africa, the americas, asia and the pacific. the world health organization (who) has recently decl ...	2016	27005721
the chikungunya virus: an emerging us pathogen.	the chikungunya (chik) virus was recently reported by the cdc to have spread to the united states. we report an early documented case of chik from the state of pennsylvania after a patient recently returned from haiti in june of 2014.	0	27006742
identification of protease m1 zinc metalloprotease conferring resistance to deltamethrin by characterization of an aflp marker in culex pipiens pallens.	continuous and excessive application of deltamethrin (dm) has resulted in the rapid development of insecticide resistance in culex pipiens pallens. the quantitative trait loci (qtl) responsible for resistance to dm had previously been detected in cx. pipiens pallens. but locating the qtls on the chromosomes remained difficult. an available approach is to first characterize dna molecular markers linked with the phenotype, and then identify candidate genes.	2016	27007119
digestion of yeasts and beta-1,3-glucanases in mosquito larvae: physiological and biochemical considerations.	aedes aegypti larvae ingest several kinds of microorganisms. in spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. we investigated the effects of using yeasts as the sole nutrient source for a. aegypti larvae. we also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. larvae were fed with cat food (control ...	2016	27007411
maternal antibody-mediated disease enhancement in type i interferon-deficient mice leads to lethal disease associated with liver damage.	epidemiological studies have reported that most of the severe dengue cases occur upon a secondary heterologous infection. furthermore, babies born to dengue immune mothers are at greater risk of developing severe disease upon primary infection with a heterologous or homologous dengue virus (denv) serotype when maternal antibodies reach sub-neutralizing concentrations. these observations have been explained by the antibody mediated disease enhancement (ade) phenomenon whereby heterologous antibod ...	2016	27007501
introduction and transmission of zika virus in brazil: new challenges for the americas.		2016	27007567
correction: how diverse detrital environments influence nutrient stoichiometry between males and females of the co-occurring container mosquitoes aedes albopictus, ae. aegypti, and culex quinquefasciatus.		2016	27007775
primary dengue virus infections induce differential cytokine production in mexican patients.	severe dengue pathogenesis is not fully understood, but high levels of proinflammatory cytokines have been associated with dengue disease severity. in this study, the cytokine levels in 171 sera from mexican patients with primary dengue fever (df) and dengue haemorrhagic fever (dhf) from dengue virus (denv) 1 (n = 116) or 2 (n = 55) were compared. df and dhf were defined according to the patient's clinical condition, the primary infections as indicated by igg enzymatic immunoassay negative resul ...	0	27008374
the retinoid x receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.	in arthropods, retinoid x receptor (rxr) is a highly conserved nuclear hormone receptor. by forming a heterodimeric complex with the ecdysone receptor (ecr), rxr is known to be vital importance for various physiological processes. however, in comparison to ecr, the rxr signaling pathway and its roles in crustacean reproduction are poorly understood. in the present study, the rxr mrna was detected in the ovarian follicular cells of mud crab scylla paramamosain (sprxr) and during ovarian maturatio ...	2016	27009370
fitting the incidence data from the city of campinas, brazil, based on dengue transmission modellings considering time-dependent entomological parameters.	four time-dependent dengue transmission models are considered in order to fit the incidence data from the city of campinas, brazil, recorded from october 1st 1995 to september 30th 2012. the entomological parameters are allowed to depend on temperature and precipitation, while the carrying capacity and the hatching of eggs depend only on precipitation. the whole period of incidence of dengue is split into four periods, due to the fact that the model is formulated considering the circulation of o ...	2016	27010654
dengue virus: a global human threat: review of literature.	dengue is an acute viral illness caused by rna virus of the family flaviviridae and spread by aedes mosquitoes. presenting features may range from asymptomatic fever to dreaded complications such as hemorrhagic fever and shock. a cute-onset high fever, muscle and joint pain, myalgia, cutaneous rash, hemorrhagic episodes, and circulatory shock are the commonly seen symptoms. oral manifestations are rare in dengue infection; however, some cases may have oral features as the only presenting manifes ...	0	27011925
virtual screening of plant volatile compounds reveals a high affinity of hylamorpha elegans (coleoptera: scarabaeidae) odorant-binding proteins for sesquiterpenes from its native host.	hylamorpha elegans(burmeister) is a native chilean scarab beetle considered to be a relevant agricultural pest to pasture and cereal and small fruit crops. because of their cryptic habits, control with conventional methods is difficult; therefore, alternative and environmentally friendly control strategies are highly desirable. the study of proteins that participate in the recognition of odorants, such as odorant-binding proteins (obps), offers interesting opportunities to identify new compounds ...	2016	27012867
identification and characterization of two "sensory neuron membrane proteins" (snmps) of the desert locust, schistocerca gregaria (orthoptera: acrididae).	pheromone-responsive neurons of insects not only require specific receptors but in addition several auxiliary components, including the "sensory neuron membrane protein," snmp. accordingly, snmp is considered as a marker for neurons responding to pheromones. for the desert locust schistocerca gregaria, it is known that the behavior, including aggregation behavior and courtship inhibition, is largely controlled by pheromones. however, little is known about pheromones, their receptors, and the phe ...	2016	27012870
the emergence of zika virus as a global health security threat: a review and a consensus statement of the indusem joint working group (jwg).	the zika virus (zikv), first discovered in 1947, has emerged as a global public health threat over the last decade, with the accelerated geographic spread of the virus noted during the last 5 years. the world health organization (who) predicts that millions of cases of zikv are likely to occur in the americas during the next 12 months. these projections, in conjunction with suspected zika-associated increase in newborn microcephaly cases, prompted who to declare public health emergency of intern ...	0	27013839
genetic variations in bionomics of culex quinquefasciatus (diptera: culicidae) mosquito population in minna, north central nigeria.	the need to have an improved knowledge on the bioecology of culex quinquefasciatus, a prerequisite in the development of cost-effective control strategies, has informed the present preliminary investigation to put in better perspective variations that exist in the egg rafts of the species. freshly laid egg rafts were collected and incubated at ambient temperature in well-labeled plastic trays. the results showed overall inconsistency in all indices monitored for the egg rafts. generally, survivo ...	2016	27013900
ambivalent outcomes of cell apoptosis: a barrier or blessing in malaria progression.	the life cycle of plasmodium in two evolutionary distant hosts, mosquito, and human, is a complex process. it is regulated at various stages of developments by a number of diverged mechanisms that ultimately determine the outcome of the disease. during the development processes, plasmodium invades a variety of cells in two hosts. the invaded cells tend to undergo apoptosis and are subsequently removed from the system. this process also eliminates numerous parasites along with these apoptotic cel ...	2016	27014225
viruses causing hemorrhagic fever. safety laboratory procedures.	viral hemorrhagic fevers are diseases caused by viruses which belong to different families, many of them causing severe diseases. these viruses may produce different symptomatology together with a severe multisystem syndrome, and the final result might be the production of hemorrhages in several sites of the body. the majority of them have no other treatment than supportive therapy, although some antiviral drugs can be used in some circumstances. transmission of vhf has been demonstrated through ...	2016	27014378
first report of leptolegnia chapmanii (peronosporomycetes: saprolegniales) affecting mosquitoes in central brazil.	numerous isolates of an oomycete 'fungus', leptolegnia chapmanii, are reported from brazil for the first time. this aquatic pathogen was baited with aedes aegypti sentinel larvae from stagnant, temporary bodies of water in selected locations under secondary tropical forest in and near the central brazilian city of goiânia and from more distant sites in the western and northern regions of the state of goiás. isolates were identified based on their morphological and developmental characters, compa ...	2016	27018147
socioeconomic and ecological factors influencing aedes aegypti prevalence, abundance, and distribution in dhaka, bangladesh.	this study examined household risk factors and prevalence, abundance, and distribution of immature aedes aegypti and aedes albopictus, and their association with socioeconomic and ecological factors at urban zonal and household levels in the city of dhaka, bangladesh. during the 2011 monsoon, 826 households in 12 randomly selected administrative wards were surveyed for vector mosquitoes. results revealed that the abundance and distribution of immature ae. aegypti and ae. albopictus, and pupae-pe ...	2016	27022149
long-term safety and immunogenicity of a tetravalent live-attenuated dengue vaccine and evaluation of a booster dose administered to healthy thai children.	we evaluated the safety and immunogenicity of two doses of a live-attenuated, tetravalent dengue virus vaccine (f17/pre formulation) and a booster dose in a dengue endemic setting in two studies. seven children (7- to 8-year-olds) were followed for 1 year after dose 2 and then given a booster dose (f17/pre formulation), and followed for four more years (child study). in the infant study, 49 2-year-olds, vaccinated as infants, were followed for approximately 3.5 years after dose 2 and then given ...	2016	27022153
characterization of a novel murine model to study zika virus.	the mosquito-borne zika virus (zikv) is responsible for an explosive ongoing outbreak of febrile illness across the americas. zikv was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with guillain-barré syndrome and microcephaly in neonates has been detected. a previous study showed that zikv requires murine adaptation to generate reproducible murine disease. in our study, a low-passage cambodian isolate caused disease and mortality in m ...	2016	27022155
rnai trigger delivery into anopheles gambiae pupae.	rna interference (rnai), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. the ability to knockdown individual genes selectively via this reverse genetic technique has allowed many researchers to rapidly uncover the biological roles of numerous genes within many organisms, by evaluation of loss-of-function phenotypes. in the major human malaria vector anopheles gambiae, the predominant me ...	2016	27023367
dengue virus-1 infection did not alter the behavioral response of aedes aegypti (diptera: culicidae) to deet.	no licensed vaccine or antiviral drug against dengue virus (denv) is available; therefore, most of the effort to prevent this disease is focused on reducing vector-host interactions. one of the most widely accepted methods of blocking vector-human contact is to use insect repellents to interfere with mosquito host-seeking behavior. some arboviruses can replicate in the nervous system of the vector, raising the concern that arboviral infection may alter the insect behavioral response toward chemi ...	2016	27026163
insect transformation with piggybac: getting the number of injections just right.	the insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggybac is the transposable element most commonly utilized for insect germline transformation. the described efficiency of this process is variable in the published literature, and a comprehensive review of transformation efficiency in insects is lacking. this study compared and contrasted ...	2016	27027400
zika virus.	zika virus (zikv) is an arthropod-borne virus (arbovirus) in the genus flavivirus and the family flaviviridae. zikv was first isolated from a nonhuman primate in 1947 and from mosquitoes in 1948 in africa, and zikv infections in humans were sporadic for half a century before emerging in the pacific and the americas. zikv is usually transmitted by the bite of infected mosquitoes. the clinical presentation of zika fever is nonspecific and can be misdiagnosed as other infectious diseases, especiall ...	2016	27029595
a pan-dengue virus reverse transcription-insulated isothermal pcr assay intended for point-of-need diagnosis of dengue virus infection by use of the pockit nucleic acid analyzer.	dengue virus (denv) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal pcr (rt-iipcr) method for the detection of all four serotypes of denv in clinical samples. the diagnostic performance of the newly established pan-denv rt-iipc ...	2016	27030492
prediction of dengue outbreaks based on disease surveillance and meteorological data.	research is needed to create early warnings of dengue outbreaks to inform stakeholders and control the disease. this analysis composes of a comparative set of prediction models including only meteorological variables; only lag variables of disease surveillance; as well as combinations of meteorological and lag disease surveillance variables. generalized linear regression models were used to fit relationships between the predictor variables and the dengue surveillance data as outcome variable on ...	2016	27031524
recombinant dengue 2 virus ns3 helicase protein enhances antibody and t-cell response of purified inactivated vaccine.	dengue virus purified inactivated vaccines (piv) are highly immunogenic and protective over the short term, but may be poor at inducing cell-mediated immune responses and long-term protection. the dengue nonstructural protein 3 (ns3) is considered the main target for t-cell responses during viral infection. the amino (n)-terminal protease and the carboxy (c)-terminal helicase domains of denv-2 ns3 were expressed in e. coli and analyzed for their immune-potentiating capacity. mice were immunized ...	2016	27035715
knowledge, attitude, and practices regarding vector-borne diseases in western jamaica.	outbreaks of vector-borne diseases (vbds) such as dengue and malaria can overwhelm health systems in resource-poor countries. environmental management strategies that reduce or eliminate vector breeding sites combined with improved personal prevention strategies can help to significantly reduce transmission of these infections.	0	27036722
mosquito host choices on livestock amplifiers of rift valley fever virus in kenya.	animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. however, these parameters remain poorly understood for mosquito vectors of the rift valley fever (rvf), an arboviral disease, and for a community of mosquitoes.	2016	27036889
identification of abcc2 as a binding protein of cry1ac on brush border membrane vesicles from helicoverpa armigera by an improved pull-down assay.	cry1ac toxin-binding proteins from helicoverpa armigera brush border membrane vesicles were identified by an improved pull-down method that involves coupling cry1ac to cnbr agarose combined with liquid chromatography-tandem mass spectrometry (lc-ms/ms). according to the lc-ms/ms results, cry1ac toxin could bind to six classes of aminopeptidase-n, alkaline phosphatase, cadherin-like protein, atp-binding cassette transporter subfamily c protein (abcc2), actin, atpase, polycalin, and some other pro ...	2016	27037552
trypsin inhibitor from leucaena leucocephala seeds delays and disrupts the development of aedes aegypti, a multiple-disease vector.	nowadays, the aedes aegypti mosquito represents a serious public health issue in view of the large outbreaks of the arboviral diseases zika, dengue, chikungunya and yellow fever. this holometabolous insect has midgut digestive enzymes that are trypsin- and chymotrypsin-like proteins. protease inhibitors are able to bind to proteolytic enzymes and promote a blockage in digestion and nutrition, leading to death. thus, we investigated the effect of trypsin inhibitor of leucaena leucocephala (lti) s ...	2017	27040615
tiger mosquito - generated devastation in the biosphere!!!		2016	27041891
can radiology play a role in early diagnosis of dengue fever?	dengue fever (df) is a viral hemorrhagic fever causing severe morbidity and mortality in affected patients.	0	27042608
tor pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in nilaparvata lugens (stål).	the "target of rapamycin" (tor) nutritional signaling pathway and juvenile hormone (jh) regulation of vitellogenesis has been known for a long time. however, the interplay between these two pathways regulating vitellogenin (vg) expression remains obscure. here, we first demonstrated the key role of amino acids (aas) in activation of vg synthesis and egg development in nilaparvata lugens using chemically defined artificial diets. aas induced the expression of tor and s6k (s6 kinase), whereas rnai ...	2016	27043527
efficacy of four commercially available heartworm preventive products against the jyd-34 laboratory strain of dirofilaria immitis.	heartworm disease in dogs can be severe and life threatening. resistance to available heartworm preventives was considered among potential causes of increased reports of failed heartworm prevention in dogs. the objective of the present study was to compare the efficacy of four commercially available heartworm disease preventives against the jyd-34 strain of d. immitis.	2016	27044379
presence of three dengue serotypes in ouagadougou (burkina faso): research and public health implications.	the significant malaria burden in africa has often eclipsed other febrile illnesses. burkina faso's first dengue epidemic occurred in 1925 and the most recent in 2013. yet there is still very little known about dengue prevalence, its vector proliferation, and its poverty and equity impacts.	2016	27044528
vaccination of gerbils with bm-103 and bm-ral-2 concurrently or as a fusion protein confers consistent and improved protection against brugia malayi infection.	the brugia malayi bm-103 and bm-ral-2 proteins are orthologous to onchocerca volvulus ov-103 and ov-ral-2, and which were selected as the best candidates for the development of an o. volvulus vaccine. the b. malayi gerbil model was used to confirm the efficacy of these ov vaccine candidates on adult worms and to determine whether their combination is more efficacious.	2016	27045170
evaluating the effectiveness of localized control strategies to curtail chikungunya.	chikungunya, a re-emerging arbovirus transmitted to humans by aedes aegypti and ae. albopictus mosquitoes, causes debilitating disease characterized by an acute febrile phase and chronic joint pain. chikungunya has recently spread to the island of st. martin and subsequently throughout the americas. the disease is now affecting 42 countries and territories throughout the americas. while chikungunya is mainly a tropical disease, the recent introduction and subsequent spread of ae. albopictus into ...	2016	27045523
identification of aadnr1, a novel gene related to innate immunity and apoptosis in aedes albopictus.	innate immunity and apoptosis play critical roles in defending pathogens in insects. in drosophila, dnr1 was reported as a negative regulator of apoptosis and immune deficiency (imd) pathway which belongs to innate immunity. aedes albopictus is an important kind of arbovirus vector and becoming a significant threat to public health due to its rapid global expansion. here we identified an ortholog of dnr1 from a. albopictus, named as aadnr1. aadnr1 encoded a putative protein containing an n-termi ...	2016	27045774
fungal biological control agents for integrated management of culicoides spp. (diptera: ceratopogonidae) of livestock.	entomopathogenic fungi metarhizium anisopliae and beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. thus, research was aimed to explore the potency of these fungal spores against larval and adult culicoides midges, a pest of livestock.	2015	27047065
risk associated with the release of wolbachia-infected aedes aegypti mosquitoes into the environment in an effort to control dengue.	in an effort to eliminate dengue, a successful technology was developed with the stable introduction of the obligate intracellular bacteria wolbachia pipientis into the mosquito aedes aegypti to reduce its ability to transmit dengue fever due to life shortening and inhibition of viral replication effects. an analysis of risk was required before considering release of the modified mosquito into the environment.	2016	27047911
larvicidal, biological and genotoxic effects, and temperature-toxicity relationship of some leaf extracts of nerium oleander (apocynaceae) on culex pipiens (diptera: culicidae).	the present study was undertaken to study the larvicidal activity of different extracts of nerium oleander leaves, and post-treatment temperature- toxicity relationship of these extracts against culex pipiens. further, the most potent extract was used to evaluate its biological and genotoxic activities.	2015	27047967
comparative pharmacological characterization of d1-like dopamine receptors from anopheles gambiae, aedes aegypti and culex quinquefasciatus suggests pleiotropic signaling in mosquito vector lineages.	small molecule antagonists of mosquito dopamine receptors (dars) are under investigation as a new class of vector-selective insecticides. antagonists that inhibit the d1-like dars aadop2 and cqdop2 from the mosquitoes aedes aegypti l. and culex quinquefasciatus say, respectively, also cause larval mortality in bioassays. here, we report on the orthologous dar, agdop2, from the malaria mosquito anopheles gambiae giles that was cloned and pharmacologically characterized in hek293 cells. larval bio ...	2016	27048546
zika virus: implications for public health.	the world health organization has declared the current zika virus (zikv) epidemic a public health emergency of international concern. lack of vaccines and reliable diagnostic tests, broad geographical distribution of mosquito species that can transmit the virus, and absence of population immunity in newly affected countries are causes for concern. although most infected persons are asymptomatic, zikv has been associated with a rise in cases of neurological complications and fetal central nervous ...	2016	27048745
genetic drift during systemic arbovirus infection of mosquito vectors leads to decreased relative fitness during host switching.	the emergence of mosquito-borne rna viruses, such as west nile virus (wnv), is facilitated by genetically complex virus populations within hosts. here, we determine whether wnv enzootic (culex tarsalis, cx. quinquefasciatus, and cx. pipiens) and bridge vectors (aedes aegypti) have differential impacts on viral mutational diversity and fitness. during systemic mosquito infection, wnv faced stochastic reductions in genetic diversity that rapidly was recovered during intra-tissue population expansi ...	2016	27049584
response of culex quinquefasciatus larvae to three insecticides.		0	27049713
biological and chemical characterization of a new larvicide ovitrap made of plastic with pyriproxyfen incorporated for aedes aegypti (diptera: culicidae) control.	aedes aegypti (l.) is a species of international concern because of its ability to transmit serious human arboviral diseases including yellow fever, dengue, and chikungunya, which have spread to all continents. ovitraps are containers constructed to imitate aedes' natural breeding sites and have been used for many decades as a sensitive and inexpensive surveillance tool for detecting the presence of container-inhabiting mosquitoes. in addition to their value for vector surveillance, various ovit ...	2016	27052297
why zika virus infection has become a public health concern?	prior to 2015, zika virus (zikv) outbreaks had occurred in areas of africa, southeast asia, and the pacific islands. although a causal relationship between zika infection during pregnancy and microcephaly is strongly suspected, such a connection has not yet been scientifically proven. in may 2015, the outbreak of zikv infection in brazil led to reports of syndrome and pregnant women giving birth to babies with birth defects and poor pregnancy outcomes; the pan american health organization (paho) ...	2016	27052792
mosquito vectors and the spread of cancer: an overlooked connection?	mosquitoes (diptera: culicidae) represent a key threat for millions of humans and animals worldwide, vectoring important pathogens and parasites, including malaria, dengue, filariasis, and zika virus. besides mosquito-borne diseases, cancers figure among the leading causes of mortality worldwide. it is expected that annual cancer cases will rise from 14 million in 2012 to 22 million within the next two decades. notably, there are few contrasting evidences of the relationship between cancer and m ...	2016	27053131
quantitative analysis of harmonic convergence in mosquito auditory interactions.	this article analyses the hearing and behaviour of mosquitoes in the context of inter-individual acoustic interactions. the acoustic interactions of tethered live pairs of aedes aegypti mosquitoes, from same and opposite sex mosquitoes of the species, are recorded on independent and unique audio channels, together with the response of tethered individual mosquitoes to playbacks of pre-recorded flight tones of lone or paired individuals. a time-dependent representation of each mosquito's non-stat ...	0	27053654
projection of climate change influences on u.s. west nile virus vectors.	while estimates of the impact of climate change on health are necessary for health care planners and climate change policy makers, models to produce quantitative estimates remain scarce. we describe a freely available dynamic simulation model parameterized for three west nile virus vectors, which provides an effective tool for studying vector-borne disease risk due to climate change. the dynamic mosquito simulation model is parameterized with species specific temperature-dependent development an ...	2015	27057131
in silico screening, alanine mutation, and dft approaches for identification of ns2b/ns3 protease inhibitors.	to identify the ligand that binds to a target protein with high affinity is a nontrivial task in computer-assisted approaches. antiviral drugs have been identified for ns2b/ns3 protease enzyme on the mechanism to cleave the viral protein using the computational tools. the consequence of the molecular docking, free energy calculations, and simulation protocols explores the better ligand. it provides in-depth structural insights with the catalytic triad of his51, asp75, ser135, and gly133. the md ...	2016	27057355
indole alkaloids of the stigonematales (cyanophyta): chemical diversity, biosynthesis and biological activity.	the cyanobacteria are well recognized as producers of a wide array of bioactive metabolites including toxins, and potential drug candidates. however, a limited number of taxa are generally considered with respect to both of these aspects. that said, the order stigonematales, although largely overlooked in this regard, has become increasingly recognized as a source of bioactive metabolites relevant to both human and environmental health. in particular, the hapalindoles and related indole alkaloid ...	2016	27058546
neglected tropical diseases in the anthropocene: the cases of zika, ebola, and other infections.		2016	27058728
harnessing the power of defensive microbes: evolutionary implications in nature and disease control.		2016	27058881
molecular cloning, sequence analysis and developmental stage expression of a putative septin gene fragment from aedes albopictus (diptera: culicidae).	septins belong to gtpases that are involved in vital cellular activities, including cytokinesis. although present in many organisms, they are yet to be isolated from aedes albopictus. this study reports for the first time on a serendipitous isolation of a partial septin sequence from ae. albopictus and its developmental expression profile. the ae. albopictus partial septin sequence contains 591 nucleotides encoding 197 amino acids. it shares homology with several insect septin genes and has a cl ...	2016	27059016
functional validation of the carbon dioxide receptor in labial palps of helicoverpa armigera moths.	adult moths possess an organ in their labial palps, the labial-palp pit organ, which is specialized for sensing carbon dioxide (co2). they use co2 as a cue to detect healthy plants and find food or lay eggs on them. the molecular bases of the co2 receptor in drosophila melanogaster and aedes aegypti have been reported, but the molecular mechanisms of the co2 receptor in lepidoptera remains elusive. in this study, we first re-examined three putative helicoverpa armigera co2 gustatory receptor gen ...	2016	27060445
homology modeling and molecular docking studies of drosophila and aedes sex peptide receptors.	the drosophila melanogaster sex peptide receptor (drmspr), which is a g protein-coupled receptor (gpcr), is known as the specific receptor for sex peptide (sp). it is responsible for the reproductive behavior in the drosophila model system; in particular, it is involved in the post-mating responses such as the increase in egg-laying ability and decrease in receptivity in females. in a previous study, we discovered a small molecule agonist of drmspr for the first time, which could not, however, a ...	2016	27060892
how to predict molecular interactions between species?	organisms constantly interact with other species through physical contact which leads to changes on the molecular level, for example the transcriptome. these changes can be monitored for all genes, with the help of high-throughput experiments such as rna-seq or microarrays. the adaptation of the gene expression to environmental changes within cells is mediated through complex gene regulatory networks. often, our knowledge of these networks is incomplete. network inference predicts gene regulator ...	2016	27065992
on the seasonal occurrence and abundance of the zika virus vector mosquito aedes aegypti in the contiguous united states.	an ongoing zika virus pandemic in latin america and the caribbean has raised concerns that travel-related introduction of zika virus could initiate local transmission in the united states (u.s.) by its primary vector, the mosquito aedes aegypti.	2016	27066299
vector surveillance for dengue virus detection in the archipelago of fernando de noronha, brazil.	aedes aegypti (l.) has become an efficient vector of important arboviruses due to its anthropophilic and domiciliary behaviors. since the 1980s, dengue affects thousands of people every year in brazil; in fernando de noronha (fn), a touristic archipelago, dengue cases have occurred since 2001. once ae. aegypti populations are well established in the inhabited areas of fn, the threat of dengue or another arbovirus epidemic is continuously imminent. this study aimed to monitor the denv serotypes i ...	2016	27067800
spectrum of maternofetal outcomes during dengue infection in pregnancy: an insight.	dengue is a vector transmitted viral infection; tropical and subtropical countries see outbreaks of dengue each year. there is a paucity of literature on effects of dengue infection on pregnancy outcome and this prompted us to undertake a study for better understanding of pregnancy implications with dengue infection. pregnant women admitted during the seasonal outbreak of dengue between september 2015 and october 2015 were studied and maternal and fetal outcomes in sixteen ns1ag positive women w ...	2016	27069349
epidemiological dynamics of an urban dengue 4 outbreak in são paulo, brazil.	dengue studies at the urban scale are scarce and required for guiding control efforts. in brazil, the burden of dengue is high and challenges city public health administrations with limited resources. here we studied the dynamics of a dengue epidemic in a single city.	2016	27069820
estimating risks of importation and local transmission of zika virus infection.	background. an international spread of zika virus (zikv) infection has attracted global attention. zikv is conveyed by a mosquito vector, aedes species, which also acts as the vector species of dengue and chikungunya viruses. methods. arrival time of zikv importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. employing a survival analysis model in which the hazard is an inverse function of the effec ...	2016	27069825
zika virus in a traveler returning to china from caracas, venezuela, february 2016.		2016	27070735
zika virus outbreak in rio de janeiro, brazil: clinical characterization, epidemiological and virological aspects.	in 2015, brazil was faced with the cocirculation of three arboviruses of major public health importance. the emergence of zika virus (zikv) presents new challenges to both clinicians and public health authorities. overlapping clinical features between diseases caused by zikv, dengue (denv) and chikungunya (chikv) and the lack of validated serological assays for zikv make accurate diagnosis difficult.	2016	27070912
computational approach towards exploring potential anti-chikungunya activity of selected flavonoids.	chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya infection in humans. despite the widespread distribution of chikv, no antiviral medication or vaccine is available against this virus. therefore, it is crucial to find an effective compound to combat chikv. we aimed to predict the possible interactions between non-structural protein 3 (nsp) of chikv as one of the most important viral elements in chikv intracellular replication and 3 potential flavonoids using a comp ...	2016	27071308
assessing potential countermeasures against the dengue epidemic in non-tropical urban cities.	dengue is a common mosquito-borne viral disease epidemic especially in tropical and sub-tropical regions where water sanitation is not substantially controlled. however, dengue epidemics sometimes occur in non-tropical urban cities with substantial water sanitary control. using a mathematical model, we investigate what conditions can be important for a dengue epidemic to occur in an urban city such as tokyo, where vectors are active only in summer and there are little number of vectors around ho ...	2016	27072122
identification and genomic analysis of a novel group c orthobunyavirus isolated from a mosquito captured near iquitos, peru.	group c orthobunyaviruses are single-stranded rna viruses found in both south and north america. until very recently, and despite their status as important vector-borne human pathogens, no group c whole genome sequences containing all three segments were available in public databases. here we report a group c orthobunyavirus, named el huayo virus, isolated from a pool of culex portesi mosquitoes captured near iquitos, peru. although initial metagenomic analysis yielded only a handful of reads be ...	2016	27074162
detection of all four dengue serotypes in aedes aegypti female mosquitoes collected in a rural area in colombia.	the aedes aegypti vector for dengue virus (denv) has been reported in urban and periurban areas. the information about denv circulation in mosquitoes in colombian rural areas is limited, so we aimed to evaluate the presence of denv in ae. aegypti females caught in rural locations of two colombian municipalities, anapoima and la mesa. mosquitoes from 497 rural households in 44 different rural settlements were collected. pools of about 20 ae. aegypti females were processed for denv serotype detect ...	0	27074252
consolidating tactical planning and implementation frameworks for integrated vector management in uganda.	integrated vector management (ivm) is the recommended approach for controlling some vector-borne diseases (vbd). in the face of current challenges to disease vector control, ivm is vital to achieve national targets set for vbd control. though global efforts, especially for combating malaria, now focus on elimination and eradication, ivm remains useful for uganda which is principally still in the control phase of the malaria continuum. this paper outlines the processes undertaken to consolidate t ...	2016	27074809
a novel video-tracking system to quantify the behaviour of nocturnal mosquitoes attacking human hosts in the field.	many vectors of malaria and other infections spend most of their adult life within human homes, the environment where they bloodfeed and rest, and where control has been most successful. yet, knowledge of peri-domestic mosquito behaviour is limited, particularly how mosquitoes find and attack human hosts or how insecticides impact on behaviour. this is partly because technology for tracking mosquitoes in their natural habitats, traditional dwellings in disease-endemic countries, has never been a ...	2016	27075002
clinical features and neuroimaging (ct and mri) findings in presumed zika virus related congenital infection and microcephaly: retrospective case series study.	to report radiological findings observed in computed tomography (ct) and magnetic resonance imaging (mri) scans of the first cases of congenital infection and microcephaly presumably associated with the zika virus in the current brazilian epidemic.	2016	27075009
one-step synthesis of polydispersed silver nanocrystals using malva sylvestris: an eco-friendly mosquito larvicide with negligible impact on non-target aquatic organisms.	the synthesis of eco-friendly nanoparticles is evergreen branch of nanoscience with a growing number of biomedical implications. in this study, we investigated the synthesis of polydisperse and stable silver nanoparticles (agnp) using a cheap leaf extract of malva sylvestris (malvaceae). bio-reduced agnp were characterized by uv-visible spectrophotometry, fourier transform infrared spectroscopy (ftir), x-ray diffraction analysis (xrd), atomic force microscopy (afm), scanning electron microscopy ...	2016	27075309
malaria vector anopheles culicifacies sibling species differentiation using egg morphometry and morphology.	the malaria vector anopheles culicifacies (sensu lato) is an important malaria vector in southeast asia which comprises of five sibling species namely a, b, c, d and e. however, only a few forms have been identified as malaria vectors in various endemic countries. currently, for the first time egg morphometry and morphology has been used to differentiate the three known vector sibling species of anopheles culicifacies collected from malaria endemic madhya pradesh state of central india.	2016	27075571
search and one will find: zika virus everywhere.		2016	27076507
profile distribution of juvenile aedes species in an urban area of malaysia.	dengue fever (df) is an urban vector-borne disease transmitted by aedes aegypti and aedes albopictus. both species deposit their eggs in favorable breeding sites either in natural or artificial containers. an understanding of their habitat characteristics is crucial in curbing df outbreaks	2016	27076510
multi-stakeholder decision aid for improved prioritization of the public health impact of climate sensitive infectious diseases.	the effects of climate change on infectious diseases are an important global health concern and necessitate decisions for allocation of resources. economic tools have been used previously; however, how prioritization results might differ when done using broader considerations identified by local stakeholders has yet to be assessed. a multicriteria decision analysis (mcda) approach was used to assess multi-stakeholder expressed concerns around disease prioritization via focus groups held in quebe ...	2016	27077875
microcephaly and zika virus: neonatal neuroradiological aspects.	the aim of this study is to describe some radiological features in the newborns with microcephaly caused by zika virus infection during pregnancy.	2016	27080092
first chikungunya outbreak in suriname; clinical and epidemiological features.	in june 2014, suriname faced the first chikungunya outbreak. since international reports mostly focus on hospitalized patients, the least affected group, a study was conducted to describe clinical characteristics of mainly outpatients including children. in addition, the cumulative incidence of this first epidemic was investigated.	2016	27082985
membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction.	understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ...	2016	27083158
analysis of significant factors for dengue fever incidence prediction.	many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the k-h model, support vector machines (svm), and artificial neural networks (ann). the time series analysis methodology, particularly arima and sarima, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. the main drawback of these methods is that they do not consider other v ...	2016	27083696
evolutionary biology and genetic techniques for insect control.	the requirement to develop new techniques for insect control that minimize negative environmental impacts has never been more pressing. here we discuss population suppression and population replacement technologies. these include sterile insect technique, genetic elimination methods such as the release of insects carrying a dominant lethal (ridl), and gene driving mechanisms offered by intracellular bacteria and homing endonucleases. we also review the potential of newer or underutilized methods ...	2015	27087849
tropical islands as new hubs for emerging arboviruses.		0	27088243
projecting month of birth for at-risk infants after zika virus disease outbreaks.	the marked increase in infants born with microcephaly in brazil after a 2015 outbreak of zika virus (zika virus) disease suggests an association between maternal zika virus infection and congenital microcephaly. to project the timing of delivery of infants born to mothers infected during early pregnancy in 1 city in bahia state, brazil, we incorporated data on reported zika virus disease cases and microcephaly cases into a graphical schematic of weekly birth cohorts. we projected that these birt ...	0	27088494
asian genotype zika virus detected in traveler returning to mexico from colombia, october 2015.		0	27088574
molecular characterization of chikungunya virus, philippines, 2011-2013.	during 2011-2013, a nationwide outbreak of chikungunya virus infection occurred in the philippines. the asian genotype was identified as the predominant genotype; sporadic cases of the east/central/south african genotype were detected in mindanao. further monitoring is needed to define the transmission pattern of this virus in the philippines.	0	27088593
clinical, virologic, and epidemiologic characteristics of dengue outbreak, dar es salaam, tanzania, 2014.	we investigated a dengue outbreak in dar es salaam, tanzania, in 2014, that was caused by dengue virus (denv) serotype 2. denv infection was present in 101 (20.9%) of 483 patients. patient age and location of residence were associated with infection. seven (4.0%) of 176 patients were co-infected with malaria and denv.	0	27088845
an operational framework for insecticide resistance management planning.	arthropod vectors transmit organisms that cause many emerging and reemerging diseases, and their control is reliant mainly on the use of chemical insecticides. only a few classes of insecticides are available for public health use, and the increased spread of insecticide resistance is a major threat to sustainable disease control. the primary strategy for mitigating the detrimental effects of insecticide resistance is the development of an insecticide resistance management plan. however, few exa ...	0	27089119
detection of autochthonous zika virus transmission in sincelejo, colombia.		0	27089253
daily rhythms in mosquitoes and their consequences for malaria transmission.	the 24-h day involves cycles in environmental factors that impact organismal fitness. this is thought to select for organisms to regulate their temporal biology accordingly, through circadian and diel rhythms. in addition to rhythms in abiotic factors (such as light and temperature), biotic factors, including ecological interactions, also follow daily cycles. how daily rhythms shape, and are shaped by, interactions between organisms is poorly understood. here, we review an emerging area, namely ...	2016	27089370
wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small rna pathways.	the intracellular endosymbiotic bacterium wolbachia can protect insects against viral infection, and is being introduced into mosquito populations in the wild to block the transmission of arboviruses that infect humans and are a major public health concern. to investigate the mechanisms underlying this antiviral protection, we have developed a new model system combining wolbachia-infected drosophila melanogaster cell culture with the model mosquito-borne semliki forest virus (sfv; togaviridae, a ...	2016	27089431
characterization of lethal zika virus infection in ag129 mice.	mosquito-borne zika virus (zikv) typically causes a mild and self-limiting illness known as zika fever, which often is accompanied by maculopapular rash, headache, and myalgia. during the current outbreak in south america, zikv infection during pregnancy has been hypothesized to cause microcephaly and other diseases. the detection of zikv in fetal brain tissue supports this hypothesis. because human infections with zikv historically have remained sporadic and, until recently, have been limited t ...	2016	27093158
lethal dengue virus infection: a forensic overview.	dengue virus is a single-stranded rna virus that is a member of the family flaviviridae, genus flavivirus. it is usually transmitted by the female aedes aegypti mosquito. dengue fever is a febrile illness caused by 1 of 4 serotypes of the virus, which may progress to dengue hemorrhagic fever or dengue shock syndrome. the mortality rate of untreated dengue shock syndrome is more than 20%. the reported incidence has increased 30-fold for the past 50 years with an estimated 50 to 100 million dengue ...	2016	27093563
the global spread of zika virus: is public and media concern justified in regions currently unaffected?	zika virus, an aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in brazil.	2016	27093860
comparative analysis of midgut bacterial communities in three aedine mosquito species from dengue-endemic and non-endemic areas of rajasthan, india.	dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. to investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely stegomyia aegypti (= aedes aegypti), fredwardsius vittatus (= aedes vittatus) and stegomyia albopicta (= aedes al ...	2016	27094337
testing the causality between cyp9m10 and pyrethroid resistance using the talen and crispr/cas9 technologies.	recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. for studies on insecticide resistance, genome editing technologies offer some advantages over the conventional reverse genetic technique, rna interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. there were relatively abundant evidences indicating that the overexpression of a cytochrome p450 gene cyp9m10 confers s ...	2016	27095599